A device for assisting the mixing of cell suspension and trypan blue
By designing a device composed of a stage, mixing hole and storage chamber, the problems of uneven mixing of cell suspension and trypan blue solution and inconvenient operation are solved, and efficient and accurate cell counting is achieved.
Patent Information
- Application Number
- CN202421119661.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-22
- Publication Date
- 2025-04-04
- Estimated Expiration
- 2034-05-22
AI Technical Summary
In the prior art, the mixing of the cell suspension and trypan blue solution is uneven, which easily produces bubbles, affects the counting accuracy, and is inconvenient to mix, resulting in low counting efficiency.
A device including a stage, a mixing hole and a storage compartment is designed to store the trypan blue solution by opening a plurality of hemispherical mixing holes on the top of the stage and storing the trypan blue solution, thereby achieving full mixing of the cell suspension and trypan blue solution.
Through this device, it is possible to effectively avoid uneven mixing and bubble generation, improve the efficiency and accuracy of cell counting, and facilitate handheld and portable.
Smart Images
Figure CN222719280U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell counting, in particular to a device for assisting the mixing of cell suspension and trypan blue. Background Art
[0002] Using a cell counter to count cells is currently a commonly used cell counting method. Compared with the traditional manual counting method under a microscope, a cell counter has the advantages of being fast, efficient, accurate, and having complete data. The current mainstream method of using a cell counter is: after fully mixing 10uL of cell suspension and 10uL of trypan blue solution, aspirate the mixed solution of 10uL of cell suspension and trypan blue solution into the entrance of the cell counting plate. After the mixed solution is fully spread, insert the cell counting plate into the cell counter for corresponding operations to obtain the desired data. Since the cell counter manufacturer does not configure the relevant mixing device for cell suspension and trypan blue solution, most users mix them locally, and the mainstream method is to spread them on a glass plate or in a small centrifuge tube for mixing. Both methods have corresponding disadvantages. For the method of mixing two solutions on a glass plate, because the amount of solution is small and on the same plane, the two solutions are prone to uneven mixing. It is also easy to generate bubbles in the pipette tip during the stage of absorbing the mixed solution, which affects the results. In addition, the cost of glass plates is relatively high and is not suitable for large-scale cell counting. For the method of mixing in a small centrifuge tube, its limitation is that the mixing operation in the centrifuge tube is inconvenient. Since the amount of the mixed liquid is too small, the centrifuge tube needs to be raised to observe the mixing. The operation of the pipette and the tip in the test tube is also inconvenient. Moreover, since the mixed solution is too little, a part of the cell suspension or trypan blue solution is easy to hang on the inner wall of the centrifuge tube, affecting the counting accuracy. The trypan blue solution is generally dispensed in a centrifuge tube. Since the 10uL pipette tip is relatively short, when the trypan blue solution is inserted into the centrifuge tube to take the trypan blue solution, the trypan blue solution hanging on the inner wall of the centrifuge tube will rub against the pipette, thereby contaminating the pipette.
[0003] Therefore, we propose a device for assisting the mixing of cell suspension and trypan blue. Utility Model Content
[0004] The utility model aims to provide a device for assisting the mixing of cell suspension and trypan blue, which solves the problems raised in the background technology.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a device for assisting the mixing of a cell suspension and trypan blue, comprising a carrier, a top of the carrier is provided with a plurality of evenly distributed mixing holes, the mixing holes are hemispherical recessed holes, and a storage bin is provided at a corner on one side of the top of the carrier.
[0006] By adopting the above technical solution, the trypan blue solution is stored in the storage bin, and then in actual use, 10uL of cell suspension and 10uL of trypan blue solution are respectively drawn into the mixing well for full mixing, and then a pipette is used to draw a mixed solution of 10uL of cell suspension and trypan blue solution into the inlet of the cell counting plate, and after the mixed solution is fully spread, the cell counting plate is inserted into the cell counter for corresponding operations to obtain various desired data, thereby improving the efficiency of cell counting.
[0007] As a preferred embodiment of the present invention, grooves are provided at both ends of the carrier, and anti-slip grooves are provided on the inner walls of the grooves.
[0008] By adopting the above technical solution, the arrangement of the groove can facilitate the moving of the device by holding the groove, making it convenient to carry and transfer.
[0009] As a preferred embodiment of the utility model, a protective cover is threadedly engaged on the surface of the storage bin.
[0010] By adopting the above technical solution and setting the protective cover, the trypan blue solution in the storage bin can be protected.
[0011] As a preferred embodiment of the present invention, a protective sticker is attached to the top opening of the mixing hole.
[0012] By adopting the above technical solution, the protective sticker is set to protect the small holes from contamination and is peeled off when in use. The sticker also has a marking function, which can avoid confusion when facing a large number of counting operations and improve the efficiency of the experiment.
[0013] As a preferred embodiment of the present invention, rubber pads are fixedly glued at the four corners of the bottom of the carrier.
[0014] By adopting the above technical solution and providing the rubber pad, the device can avoid sliding when placed in a placement location for use.
[0015] Compared with the prior art, the beneficial effects of the utility model are as follows:
[0016] The technical solution of the present application is a device for assisting in mixing a cell suspension and trypan blue. The cell suspension and the trypan blue solution can be fully mixed through a hemispherical hole with a diameter of 5 mm and a depth of 0.25 mm, and it is convenient for pipette operation and will not contaminate the pipette. A protective sticker is provided above the hole to protect the hole from contamination, which is removed during use. The sticker also has a marking function, which can avoid confusion when facing a large number of counting operations and improve the efficiency of the experiment. The trypan blue storage bin is designed to have a larger opening and a shallower depth, which is suitable for use with a 10uL range pipette, and can prevent the pipette from rubbing against the trypan blue solution and contaminating the pipette. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Other features, objects and advantages of the present invention will become more apparent by reading the detailed description of non-limiting embodiments with reference to the following drawings:
[0018] Figure 1 This is a schematic diagram of the overall structure of the device for assisting the mixing of cell suspension and trypan blue according to the utility model;
[0019] Figure 2 The utility model is a schematic diagram of the cross-sectional structure of a device for assisting the mixing of a cell suspension and trypan blue.
[0020] In the figure:
[0021] 1. Carrier; 11. Mixing hole; 12. Storage compartment; 13. Protective cover; 14. Protective sticker; 15. Rubber pad; 16. Groove. DETAILED DESCRIPTION
[0022] See also Figure 1-2 The utility model provides a technical solution: a device for assisting the mixing of a cell suspension and trypan blue, comprising a carrier 1, a plurality of evenly distributed mixing holes 11 are provided on the top of the carrier 1, the mixing holes 11 are hemispherical concave holes, with a diameter of 5 mm and a depth of 0.25 mm, and a storage bin 12 is provided at a corner on one side of the top of the carrier 1;
[0023] In actual use, the trypan blue solution is stored in the storage bin 12, and then in actual use, 10uL of cell suspension and 10uL of trypan blue solution are respectively drawn into the mixing hole 11 for full mixing, and then the mixed solution of 10uL of cell suspension and trypan blue solution is drawn into the inlet of the cell counting plate by a pipette, and after the mixed solution is fully spread, the cell counting plate is inserted into the cell counter for corresponding operations to obtain various desired data, thereby improving the efficiency of cell counting;
[0024] Furthermore, a protective cover 13 is threadedly engaged on the surface of the storage bin 12, and the setting of the protective cover 13 can protect the trypan blue solution in the storage bin 12;
[0025] Furthermore, a protective sticker 14 is attached to the top opening of the mixing hole 11. The protective sticker 14 protects the small hole from contamination and is peeled off when in use. The sticker also has a marking function, which can avoid confusion when facing a large number of counting operations and improve the efficiency of the experiment.
[0026] It is worth mentioning that rubber pads 15 are fixedly glued at the four corners of the bottom of the carrier 1. The provision of the rubber pads 15 prevents the device from sliding when it is placed in a placement location for use.
[0027] like Figure 1As shown; both ends of the carrier 1 are provided with grooves 16, and the inner wall of the groove 16 is provided with anti-slip grooves. The setting of the groove 16 can facilitate the hand-held groove 16 to move the device, making it easy to carry and transfer.
[0028] The implementation principle of the device for assisting the mixing of cell suspension and trypan blue in the present application is as follows: in actual use, the trypan blue solution is stored in the storage bin 12, and then in actual use, 10uL of cell suspension and 10uL of trypan blue solution are respectively sucked and introduced into the mixing hole 11 for sufficient mixing, and then the mixed solution of 10uL of cell suspension and trypan blue solution is sucked into the entrance of the cell counting plate by using a pipette, and after the mixed solution is fully spread, the cell counting plate is inserted into the cell counter for corresponding operations to obtain various desired data, thereby improving the efficiency of cell counting, and when transfer is required, the device is moved by holding the groove 16, so that it is convenient to carry and transfer.
[0029] In addition, the components included in the device for assisting the mixing of cell suspension and trypan blue in the utility model are all universal standard parts or components known to technical personnel in this field, and their structures and principles can be known to technical personnel in this field through technical manuals or through conventional experimental methods. In the idle part of this device, all the above-mentioned electrical components, which refer to power elements, electrical components, and adapted monitoring computers and power supplies, are connected through wires. The specific connection means should refer to the following working principle, and the electrical connection is completed in the working order of each electrical component. The detailed connection means are well-known technologies in this field. The following mainly introduces the working principle and process, and does not explain the electrical control.
Claims
1. A device for assisting the mixing of a cell suspension and trypan blue, comprising a carrier (1), characterized in that: The top of the carrier (1) is provided with a plurality of evenly distributed mixing holes (11), wherein the mixing holes (11) are hemispherical recessed holes, and a storage bin (12) is provided at a corner on one side of the top of the carrier (1).
2. A device for assisting the mixing of a cell suspension and trypan blue according to claim 1, characterized in that: Grooves (16) are provided at both ends of the carrier (1), and anti-slip grooves are provided on the inner walls of the grooves (16).
3. A device for assisting the mixing of a cell suspension and trypan blue according to claim 1, characterized in that: A protective cover (13) is threadedly engaged on the surface of the storage bin (12).
4. The device for assisting the mixing of a cell suspension and trypan blue according to claim 1, characterized in that: A protective sticker (14) is attached to the top opening of the mixing hole (11).
5. The device for assisting the mixing of a cell suspension and trypan blue according to claim 1, characterized in that: Rubber pads (15) are fixedly glued at the four corners of the bottom of the carrier (1).