A sample heating incubation baking sheet and immunohistochemistry instrument

Abstract

The present application relates to a sample heating incubation baking sheet and an immunohistochemistry instrument, and relates to the technical field of experimental instruments. A sample heating incubation baking sheet comprises a heat-conducting shell, a heating plate and a temperature sensor. A mounting groove is provided on one side of the heat-conducting shell, and the heating plate is attached to the bottom surface of the mounting groove. A mounting hole is provided on the heating plate, and the temperature sensor is placed in the mounting hole and abuts against the bottom surface of the mounting groove. In the present application, the temperature sensing surface of the temperature sensor is in direct contact with the heat-conducting shell, which has the effect of being able to more accurately detect the temperature of the heat-conducting shell.

Description

Technical Field

[0001] The present application relates to the technical field of experimental instruments, and in particular to a sample heating and incubation baking sheet and an immunohistochemical instrument. Background Art

[0002] Immunohistochemistry is an instrument used to perform immunohistochemistry experiments. Immunohistochemistry is a technique used to detect the expression of specific proteins in tissues or cells by using specific antibodies to label the target protein and then using chemical methods to make it visible.

[0003] The immunohistochemical instrument includes a sample heating and incubation baking sheet, which is mainly used to provide a constant temperature to promote the binding reaction between antibodies and target proteins. This device is usually used in the staining step of immunohistochemical experiments to help accelerate the staining reaction and enhance the stability of staining.

[0004] The sample heating incubation baking sheet mainly includes a heating sheet, a heat-conducting shell and a temperature sensor, wherein the heating sheet is attached to the heat-conducting shell to heat the heat-conducting shell, and the temperature sensor is arranged on the heating sheet to monitor the real-time temperature of the heat-conducting shell. When the experimental vessel needs to be heated, the experimental vessel can be placed on the heat-conducting shell.

[0005] However, in the process of heating the experimental vessel, the heating plate has heat loss when transferring heat to the heat-conducting shell, resulting in a temperature difference between the heating plate and the heat-conducting shell. The temperature sensor is arranged on the side of the heating plate away from the heat-conducting shell, so that the temperature sensor cannot accurately detect the temperature in real time. Utility Model Content

[0006] The purpose of the present application is to provide a sample heating incubation baking sheet and immunohistochemistry instrument with more accurate detection accuracy.

[0007] In the first aspect, the present application provides a sample heating and incubation baking sheet using the following technical solution:

[0008] A sample heating incubation baking sheet comprises a heat-conducting shell, a heating sheet and a temperature sensor. A mounting groove is provided on one side of the heat-conducting shell. The heating sheet is attached to the bottom surface of the mounting groove. A mounting hole is provided on the heating sheet. The temperature sensor is placed in the mounting hole and abuts against the bottom surface of the mounting groove.

[0009] By adopting the above technical solution, the heating plate first heats the heat-conducting shell, and the heat-conducting shell is used as a device for directly heating the experimental vessel, and the temperature sensing surface on the temperature sensor directly acts on the groove wall of the installation groove. When the temperature of the heat-conducting shell changes, the temperature sensor can respond more quickly, so that the resistance value of the temperature sensor changes, so as to adjust the power of the heating plate, and compared with the monitoring method in which the temperature sensing surface acts on the heating plate, the adjustment accuracy is higher.

[0010] Optionally, the heating plate includes a heating wire and a diaphragm covering the heating wire, and the heating wire is S-shaped and spreads inside the diaphragm.

[0011] By adopting the above technical solution, the heating wires are evenly distributed in the diaphragm to improve the heating effect.

[0012] Optionally, a portion of the heating wire close to the edge of the diaphragm is thinner than a portion away from the edge of the diaphragm.

[0013] By adopting the above technical solution, the heating wire heats the heat-conducting shell, and the heating wire located at the edge of the diaphragm is thinner than the heating wire on the inside of the diaphragm. Under the same material, the thinner heating wire has a larger resistance value, so that the heating power of the heating wire located on the outside is higher than that of the heating wire on the inside. When the heat is dissipated outward, the uniform distribution of heat can be guaranteed.

[0014] Optionally, the sample heating incubation baking sheet further comprises a flexible heat-conductive layer, one side of the flexible heat-conductive layer is adhered to the bottom wall of the mounting groove; the other side of the flexible heat-conductive layer is provided with a receiving groove for accommodating the heating wire, and is adhered to the heating sheet.

[0015] Optionally, a flexible heat-conducting layer is provided between the heating wires and the heat-conducting shell, and two side surfaces of the flexible heat-conducting layer are respectively bonded to the heating wires and the heat-conducting shell.

[0016] By adopting the above technical solution, a flexible heat-conductive layer is set to fill the gap between the heating wire and the mounting groove, so as to ensure that the heating wire fits the groove wall of the mounting groove more closely and reduce the interference of the gap on temperature transfer. Therefore, under the same heating conditions, a tighter fit will help improve the heating efficiency and make the temperature distribution more uniform.

[0017] Optionally, a positioning hole is provided on the heating plate, and a positioning column which can be inserted into the positioning hole is provided on the bottom wall of the mounting groove for positioning the heating plate.

[0018] By adopting the above technical solution, the positioning column cooperates with the positioning hole to position the heating plate, and the position of the resistance heating plate is offset during operation, resulting in uneven heating of the experimental vessel.

[0019] Optionally, a pressing block is further included, wherein the pressing block is connected to the positioning column and is used to fix the heating plate in the installation groove.

[0020] By adopting the above technical solution, the pressing block is arranged to cooperate with the positioning column, so that the heating plate can be better fixed.

[0021] Optionally, the pressing block is made of high-temperature material.

[0022] By adopting the above technical solution, the high temperature resistance of the pressing block is improved, and damage and deformation due to long-term exposure to high temperature conditions are prevented.

[0023] Optionally, the temperature sensor includes a body, pins and solder joints, the solder joints are connected to the body via the pins, and the pressing block is correspondingly provided with a second avoidance hole for avoiding the pins and the solder joints.

[0024] By adopting the above technical solution, the second avoidance hole is opened to accommodate the pins and the solder joints, so as to facilitate the installation of the pressing block, so that the pressing block can better press the heating plate.

[0025] Optionally, a sealing block is filled in the second avoidance hole, and one side of the sealing block surrounds the back side of the temperature sensor to seal the second avoidance hole.

[0026] By adopting the above technical solution, the sealing block presses the temperature sensor to ensure that the temperature sensor can fit completely and tightly on the groove wall of the installation groove, directly collect the temperature of the heat-conducting shell, and at the same time avoid the temperature sensor from contacting with water vapor in the air, which may cause problems such as easy damage.

[0027] Optionally, a sealing ring is provided in the groove wall of the installation groove, and the sealing ring is pressed on the outer ring of the heating plate to seal the periphery of the heating plate.

[0028] By adopting the above technical solution and providing a sealing ring, water vapor in the air can be further prevented from entering through a possible gap between the heating plate and the heat-conducting shell, thereby preventing the heating plate and the temperature sensor from being damaged by moisture.

[0029] In the second aspect, an immunohistochemical instrument is provided that adopts the following technical solution:

[0030] An immunohistochemical instrument comprises the above-mentioned sample heating and incubation baking sheet.

[0031] By adopting the above technical solution, the sample incubation baking device provides a constant temperature and humidity environment for the immunohistochemistry instrument to promote the binding reaction between the antibody and the target protein, help accelerate the staining reaction and enhance the stability of the staining.

[0032] In summary, the present application includes at least one of the following beneficial technical effects:

[0033] 1. The temperature sensor in the present application directly acts on the wall of the mounting groove. When the temperature of the heat-conducting shell changes, the temperature sensor can respond more quickly, causing the resistance of the temperature sensor to change, so as to adjust the power of the heating plate. Compared with the monitoring method in which the temperature sensor acts on the heating plate, the adjustment accuracy is higher.

[0034] 2. The present application sets a flexible heat-conductive layer to fill the gap between the heating wire and the mounting groove, so as to ensure that the heating wire fits more closely to the groove wall of the mounting groove and reduce the interference of the gap on temperature transfer. Therefore, under the same heating conditions, a tighter fit will help improve the heating efficiency and make the temperature distribution more uniform.

[0035] 3. In the present application, a sealing block and a sealing ring are provided so that the sealing block seals the heating plate, thereby preventing possible moisture from entering between the heating plate and the heat-conducting shell, and preventing the heating plate and the temperature sensor from being damaged by moisture. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a schematic diagram of the three-dimensional structure of the sample heating and incubation baking sheet in Example 1 of the present application;

[0037] Figure 2 is a schematic cross-sectional structural diagram of the sample heating and incubation baking sheet in Example 1 of the present application;

[0038] Figure 3 It is a schematic diagram of the three-dimensional structure of the heating plate and the heating wire in Example 1 of the present application;

[0039] Figure 4 It is a schematic diagram of the explosion structure of the sample heating and incubation baking sheet in Example 1 of the present application;

[0040] Figure 5 This application Figure 4 A partial enlarged schematic diagram of part A;

[0041] Figure 6 It is a schematic diagram of the cross-sectional structure of the sample heating and incubation baking sheet in Example 2 of the present application.

[0042] In the figure, 1, heat-conducting shell; 11, mounting groove; 2, heating plate; 21, mounting hole; 22, heating wire; 23, diaphragm; 24, positioning hole; 3, temperature sensor; 31, body; 32, pin; 33, solder joint; 4, sealing ring; 5, flexible heat-conducting layer; 51, receiving groove; 6, positioning column; 7, pressure block; 71, second avoidance hole; 8, protective layer; 81, first avoidance hole; 9, sealing block; 20, conductive part; 30, plug; 40, fastening screw; 50, fixing sleeve. DETAILED DESCRIPTION

[0043] The following is combined with Figure 1 -Attached Figure 6 , further details of this application are given.

[0044] Embodiment 1:

[0045] An immunohistochemical instrument, including a sample heating and incubation baking sheet, referring to Figure 1 and Figure 2 The sample heating incubation baking sheet includes a heat-conducting shell 1, a heating sheet 2 and a temperature sensor 3 for controlling the temperature of the heating sheet 2. A mounting groove 11 is provided on one side of the heat-conducting shell 1. The heating sheet 2 is attached to the groove wall of the mounting groove 11. Figure 3 A mounting hole 21 is formed through the center of the heating plate 2. The temperature sensor 3 is electrically connected to the heating plate 2 and is placed in the mounting hole 21. One side of the temperature sensor 3 has a temperature sensing surface (a side of the temperature sensor close to the heat-conducting shell 1), and the temperature sensing surface abuts against the groove wall of the mounting groove 11.

[0046] Combination Figure 3 and Figure 4 The heating plate 2 includes a heating wire 22 and a diaphragm 23 covering the heating wire. The heating wire 22 is S-shaped and spreads throughout the interior of the diaphragm 23 to heat the heat-conducting shell 1. Furthermore, since the heating wire 22 dissipates heat outward during the heating process, the heat dissipation of the heating wire 22 close to the edge of the diaphragm 23 is most serious. In this embodiment, the portion of the heating wire 22 close to the edge of the diaphragm 23 is thinner than the portion far from the edge of the diaphragm 23.

[0047] Under the same material, the thinner heating wire 22 has a greater resistance value, so that the heating power of the heating wire 22 located on the outside is higher than that of the heating wire 22 on the inside, and when the heat is dissipated outward, the uniform distribution of the heat can be ensured.

[0048] Furthermore, since the heating wire 22 has a volume, the portion where the diaphragm contacts the heating wire is convex, and when the heating plate 2 is fixed on the heat-conductive shell 1, a gap will be formed between the bottom surface of the mounting groove 11. In the present embodiment, the sample heating incubation baking sheet also includes a flexible heat-conductive layer 5, one side of the flexible heat-conductive layer 5 is attached to the bottom wall of the mounting groove 11; the other side of the flexible heat-conductive layer 5 is attached to the heating plate 2 to form a receiving groove 51 for accommodating the heating wire 22.

[0049] In this embodiment, the flexible heat-conducting layer 5 is made of 3M double-sided tape to further fix the heating plate 2 on the heat-conducting shell 1 while ensuring the heat-conducting effect. In other embodiments, the flexible heat-conducting layer can also be made of cloth or silicone material.

[0050] By providing a flexible heat-conducting layer 5, the gap between the heating plate 2 and the bottom surface of the mounting groove 11 is filled, the proportion of the cavity is reduced, and the heating wire 22 is more closely attached to the heat-conducting shell 1, which reduces the interference of the cavity on the temperature transfer. In addition, the flexible heat-conducting layer 5 itself also has thermal conductivity, which ensures that the heating wire 22 can heat and transfer heat to the heat-conducting shell 1 more evenly.

[0051] It should be noted that, in this embodiment, the side of the heating plate 2 facing away from the heat-conducting shell 1 also has a heating wire 22 covered by a diaphragm 23 to enhance the heating effect and increase the heating rate of the heat-conducting shell 1 .

[0052] Reference Figure 2 and Figure 4 A positioning hole 24 is provided on the heating plate 2, and a positioning column 6 which can be inserted into the positioning hole 24 is provided on the bottom wall of the mounting groove 11 for positioning the heating plate 2. When the heating plate 2 is attached to the heat-conducting shell 1, the positioning column 6 is aligned with the positioning hole 24 to complete the positioning of the heat-conducting shell 1 on the heating plate 2 and prevent the heating plate 2 from being offset.

[0053] Further, refer to Figure 1 and Figure 2 , and also includes a pressing block 7, which is connected to the positioning column 6 and is used to fix the heating plate 2 in the installation groove 11. In this embodiment, the pressing block 7 and the positioning column 6 are threadedly connected by a fastening screw 40. The pressing block 7 has a fixing sleeve 50 that can be sleeved on the outer end of the positioning column 6 to facilitate the cooperation between the positioning column 6 and the pressing block 7. In other embodiments, the pressing block 7 can also be clamped with the positioning column 6 to ensure that the pressing block 7 can fix the heating plate 2 in the installation groove 11.

[0054] It should be noted that the pressing block 7 is made of high-temperature resistant material to prevent damage and deformation due to long-term high-temperature conditions. In this embodiment, the pressing block 7 is made of high-temperature resistant plastic material. In other embodiments, the pressing block 7 can also be made of high-temperature resistant metal material or ceramic material.

[0055] When the heating plate 2 is initially fixed on the heat-conducting shell 1, the fixing sleeve 50 is aligned with the positioning column 6, and the positioning column 6 is inserted into the fixing sleeve 50 from one side of the fixing sleeve 50. Then, the fastening screw 40 is inserted into the fixing sleeve 50 from the other side of the fixing sleeve 50 and threadedly engaged with the positioning hole 24. When the fastening is completed, the side walls of the pressing block 7 are respectively pressed against the heating plate 2 and the temperature sensor 3. Since the flexible heat-conducting layer 5 is elastic, the flexible heat-conducting layer 5 is squeezed in the process of fixing the heating plate 2 by the pressing block 7, thereby further reducing the gap formed between the heating plate 2 and the heating wire 22.

[0056] Furthermore, a plurality of positioning posts 6 are provided. In the present embodiment, there are two positioning posts 6, which are fixed on the side wall of the mounting groove 11 along the length direction of the heat-conducting shell 1. The positioning posts 6 and the heat-conducting shell 1 are integrally formed by a mold. Correspondingly, two groups of fixing sleeves 50 and fastening screws 40 are also provided, and are arranged one-to-one with the two positioning posts 6.

[0057] In addition, the inner circle of the fixing sleeve 50 on the side facing away from the heating plate 2 is provided with a rounded corner to facilitate the insertion of the fastening screws 40 .

[0058] Reference Figure 4 and Figure 5 The temperature sensor 3 includes a body 31, a pin 32 and a soldering point 33. The body 31 and the soldering point 33 are connected through the pin 32. There are two soldering points 33 and two pins 32. The two soldering points 33 are arranged on the pad in the center of the heating plate 2, and are arranged one by one with the two pins 32. Further, the temperature sensor 3 adopts a four-wire PT1000. The PT1000 has four leads, two of which are used to collect ADC, and the remaining two are used to form a current loop. In this embodiment, a lead for collecting ADC and a lead for forming a current loop are grouped together, and the soldering point 33 is electrically connected to a group of leads and a pin 32 respectively.

[0059] Through current excitation and voltage measurement, the wire resistance is compensated to eliminate the line-to-line error, thereby more accurately collecting the temperature of the heat-conducting shell 1 .

[0060] It should be noted that a conductive part 20 extends from one side of the heating plate 2, one side of the conductive part 20 is electrically connected to the heating wire 22 and the temperature sensor 3 respectively, and the other side of the guide part has a plug 30 for connecting to a power source.

[0061] Reference Figure 2 and Figure 4 A second avoidance hole 71 for avoiding pins and solder joints is provided in the center of the pressing block 7 to facilitate the installation of the temperature sensor 3. In addition, the second avoidance hole 71 is filled with a sealing block 9, one side of the sealing block 9 surrounds the back side of the temperature sensor 3 (the side of the temperature sensor 3 facing away from the heat-conducting shell 1) to seal the second avoidance hole 71, prevent water vapor in the air from contacting the temperature sensor 3 and press the temperature sensor 3, so as to ensure that the temperature sensor 3 and the heat-conducting shell 1 are tightly fitted.

[0062] Furthermore, a sealing ring 4 is arranged in the groove wall of the installation groove 11, and the sealing ring 4 is pressed on the outer ring of the heating plate 2 to seal the periphery of the heating plate 2 to prevent a gap from being formed between the heating plate 2 and the heat-conducting shell 1, thereby causing water vapor to enter.

[0063] In this embodiment, the sealing block 9 and the sealing ring 4 are made of the same material and are both formed by resin solidification. The resin is coated around the edge of the heating plate 2 to form a sealing ring 4 for sealing the edge area of ​​the heating plate 2. The resin is injected into the second avoidance hole 71 for potting to form a sealing block 9 for sealing the second avoidance hole 71 to prevent moisture from entering the gap formed between the heating plate 2 and the heat-conducting shell 1, and to prevent the temperature sensor 3 and the heating wire 22 from getting damp. It should be noted that when forming the sealing block 9 and the sealing ring 4, a gradient temperature is used to heat and cure the resin.

[0064] The implementation principle of Example 1 of the present application is:

[0065] When monitoring the temperature, the temperature sensing surface of the temperature sensor 3 is directly in contact with the heat-conducting shell 1, the temperature of the heat-conducting shell 1 is monitored and timely feedback is given, and the temperature sensor 3 adopts a four-wire PT1000, and the temperature detection is more accurate and direct. A flexible heat-conducting layer 5 is provided on one side of the heating plate 2 to fill the gap formed by the heating wire 22 and transfer the heat generated by the heating wire 22 to the heat-conducting shell 1, and a pressing block 7 is provided on the other side of the heating plate 2 to press the heating plate 2, further making the heating wire 22 close to the heat-conducting shell 1, so that the heat-conducting shell 1 is heated more quickly and more evenly.

[0066] Embodiment 2:

[0067] Reference Figure 6 The difference between the present embodiment 2 and the embodiment 1 is that a protective layer 8 is provided between the pressing block 7 and the heating plate 2, and a first avoidance hole 81 for avoiding the temperature sensor 3 and the positioning column 6 is opened on the protective layer 8, and the two sides of the protective layer 8 are respectively in contact with the pressing block 7 and the heating plate 2.

[0068] The protective layer 8 enables indirect contact between the pressing block 7 and the heating plate 2 to protect the heating plate 2, and the heating plate 2 is pressed by the combination of the protective layer 8 and the pressing block 7 to ensure that the temperature sensing surface of the temperature sensor 3 can be completely and tightly attached to the groove wall of the mounting groove 11. In addition, in this embodiment, the protective layer 8 is made of flexible material, and can be made of silicone material or cloth. The thickness of the protective layer 8 is 0.8 mm to avoid the combination of the pressing block 7 and the thermal grease sheet being too thick and causing a burden.

[0069] The implementation principle of Example 2 of the present application is:

[0070] When the pressing block 7 presses the heating plate 2 , the protective layer 8 can protect the heating plate 2 to prevent the heating plate 2 from being damaged by the pressing block 7 .

[0071] The embodiments of this specific implementation are all preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. The same components are represented by the same figure marks. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A sample heating and incubation baking sheet, comprising a heat-conducting shell (1), a heating sheet (2) and a temperature sensor (3), characterized in that: A mounting groove (11) is provided on one side of the heat-conducting shell (1); the heating plate (2) is attached to the bottom surface of the mounting groove (11); a mounting hole (21) is provided on the heating plate (2); the temperature sensor (3) is placed in the mounting hole (21) and abuts against the bottom surface of the mounting groove (11).

2. The sample heating and incubation baking sheet according to claim 1, characterized in that: The heating plate (2) comprises a heating wire (22) and a diaphragm (23) covering the heating wire (22); the heating wire (22) is arranged in an S-shaped manner and is spread throughout the interior of the diaphragm (23).

3. The sample heating and incubation baking sheet according to claim 2, characterized in that: The portion of the heating wire (22) close to the edge of the diaphragm (23) is thinner than the portion away from the edge of the diaphragm (23); and / or The sample heating incubation baking sheet further comprises a flexible heat-conducting layer (5), one side of the flexible heat-conducting layer (5) being in contact with the bottom wall of the mounting groove (11); the other side of the flexible heat-conducting layer (5) is provided with a receiving groove (51) for receiving the heating wire (22) and in contact with the heating sheet (2).

4. The sample heating and incubation baking sheet according to claim 1, characterized in that: The heating plate (2) is provided with a positioning hole (24), and the bottom wall of the mounting groove (11) is provided with a positioning column (6) which can be inserted into the positioning hole (24) and is used to position the heating plate (2).

5. The sample heating and incubation baking sheet according to claim 4, characterized in that: It also comprises a pressing block (7), wherein the pressing block (7) is connected to the positioning column (6) and is used to fix the heating plate (2) in the installation groove (11).

6. The sample heating and incubation baking sheet according to claim 5, characterized in that: The pressing block (7) is made of high temperature resistant material.

7. The sample heating and incubation baking sheet according to claim 5, characterized in that: The temperature sensor (3) comprises a body (31), a pin (32) and a soldering point (33); the soldering point (33) is connected to the body (31) via the pin (32); and the pressing block (7) is provided with a corresponding second avoidance hole (71) for avoiding the pin (32) and the soldering point (33).

8. The sample heating and incubation baking sheet according to claim 7, characterized in that: A protective layer (8) is provided between the pressing block (7) and the body of the temperature sensor (3).

9. The sample heating and incubation baking sheet according to claim 7, characterized in that: The second avoidance hole (71) is filled with a sealing block (9), one side of the sealing block (9) surrounds the back side of the temperature sensor (3) to seal the second avoidance hole (71); and / or A sealing ring (4) is arranged in the groove wall of the installation groove (11), and the sealing ring (4) is pressed on the outer ring of the heating plate (2) to seal the periphery of the heating plate (2).

10. An immunohistochemical instrument, characterized in that: It comprises the sample heating and incubation baking sheet as described in any one of claims 1 to 9.

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