A natural protein purification device

Abstract

The utility model discloses a natural protein purification device, including a support frame, a heating tube is rotatably connected between the support frames, a stirring assembly is provided inside the purification box, a filtering assembly is provided at the bottom of the stirring assembly, a swinging assembly is provided at the bottom of the filtering assembly, the stirring assembly includes a hollow rod rotatably connected inside the purification box, a top fixed sleeve of the hollow rod is provided with a feeding hopper, and an external fixed sleeve of the hollow rod is provided with a driven gear. The utility model drives the driving gear to rotate through the output shaft of the driving motor, thereby rotating the driven gear meshing with the driving gear, and then driving the hollow rod to rotate synchronously during the rotation process, and then under the action of centrifugation, the organic solvent is led out through the drip tube outside the hollow rod, and the organic solvent is contacted with the protein liquid inside the purification box, and the protein liquid inside the purification box and the organic solvent are stirred by the stirring rod outside the hollow rod.

Description

Technical Field

[0001] The utility model belongs to the technical field of purification devices, and in particular relates to a natural protein purification device. Background Art

[0002] Protein purification is widely used in biochemical research applications and is an important operating technique. In order to study a certain protein, the protein must first be purified from other proteins and non-protein molecules.

[0003] Organic solvent precipitation is one of the main methods for purifying plant protein liquid. The protein can be precipitated by using organic solvents such as water-soluble non-ionic polymers. The existing precipitation method for purifying protein liquid is slow, and no filter plate assembly is provided. The filter plate cannot be replaced as needed, resulting in poor purification purity. Therefore, improvements are now made to a natural protein purification device. Utility Model Content

[0004] In order to overcome the above-mentioned defects of the prior art, an embodiment of the utility model provides a natural protein purification device, which has a higher protein extraction efficiency and convenient filter plate replacement through the mutual cooperation of a stirring component and a filtering component, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the utility model proposes a natural protein purification device, comprising a support frame, a purification box is rotatably connected between the support frames, a stirring component is arranged inside the purification box, a filtering component is arranged at the bottom of the stirring component, and a swing component is arranged at the bottom of the filtering component;

[0006] The stirring assembly includes a hollow rod rotatably connected to the inside of a purification box, a top fixed sleeve of the hollow rod is provided with a feeding hopper, an outer fixed sleeve of the hollow rod is provided with a driven gear, a fixed frame is fixedly installed on the top of the purification box, a driving motor is fixedly installed on the top of the fixed frame, an output shaft of the driving motor passes through one side of the fixed frame and is connected to a driving gear, the driving gear is meshed with the driven gear for transmission, a plurality of drip tubes are provided on the outer fixed sleeve of the hollow rod, and a plurality of stirring rods are provided on the outside of the hollow rod.

[0007] Preferably, the filter assembly includes a plug-in slot opened inside the purification box, two slide rails are provided on the side of the purification box close to the plug-in slot, a filter plate is slidably connected between the two slide rails, a limit block is installed on the side of the purification box close to the filter plate, a through slot is opened inside the limit block, a spring is installed inside the through slot, one end of the spring is connected to a slide rod, a drag plate is fixedly connected to the outside of the slide rod, a fixing plate is installed on the side of the filter plate close to the slide rod, a fixing hole is opened inside the fixing plate, and the slide rod passes through one side of the limit block and is movably connected to the inside of the fixing hole.

[0008] Preferably, the swing assembly includes a sector gear fixedly mounted at the bottom center of the purification box, a servo motor is mounted on the support frame, an output shaft of the servo motor is fixedly connected to a circular gear, and the circular gear is meshed with the sector gear for transmission.

[0009] Preferably, an observation panel is provided on the outside of the purification box, a discharge pipe is provided on the bottom fixed sleeve of the purification box, and a liquid inlet pipe is provided on the top fixed sleeve of the purification box.

[0010] Preferably, the internal fixed sleeve of the purification box is provided with a mounting plate, the internal fixed sleeve of the mounting plate is provided with a flow guide tube, the interior of the flow guide tube is fixedly installed with a solenoid valve, the outside of the purification box is installed with a controller, and the solenoid valve is electrically connected to the controller.

[0011] Preferably, a purity detector is installed on the top of the mounting plate, and the purity detector is electrically connected to the controller.

[0012] Preferably, rotating rods are fixedly installed on both sides of the purification box, a bearing is fixedly installed on one side of the support frame close to the rotating rod, and the rotating rod is fixedly sleeved in the bearing.

[0013] The natural protein purification device proposed by the utility model can bring the following beneficial effects:

[0014] 1. The output shaft of the driving motor drives the driving gear to rotate, and then the driven gear meshing with the driving gear rotates, and then the hollow rod is driven to rotate synchronously during the rotation process. Under the action of centrifugation, the organic solvent is discharged through the drip tube outside the hollow rod, and the organic solvent is brought into contact with the protein liquid inside the purification box, and the protein liquid and the organic solvent inside the purification box are stirred by the stirring rod outside the hollow rod.

[0015] 2. The output shaft of the servo motor drives the circular gear to rotate repeatedly clockwise and counterclockwise, so that the circular gear drives the fan gear to engage in transmission, and then the purification box swings in the bearing through the rotating rod, so that the organic solvent and protein liquid inside the purification box are fully mixed and reacted, thereby accelerating the precipitation speed.

[0016] 3. The staff can observe the situation of the protein liquid and the organic solvent inside the purification box through the controller, so as to facilitate the timely purification of the protein liquid. After precipitation, the solenoid valve inside the guide tube is opened through the controller to discharge the protein liquid. After the reaction, the protein liquid passes through the filter plate, and the precipitate produced by the reaction of the protein liquid and the organic solvent is filtered, and finally the purified protein liquid is discharged through the drip tube.

[0017] 4. Insert the filter plate into the plug-in slot and push the filter plate inward through the plug-in slot, so that both ends of the filter plate slide along the slide rails. When the fixed plate contacts the end of the slide rod, the spring contracts due to extrusion. When the end of the slide rod contacts the fixed hole inside the fixed plate, the reaction force of the spring drives the end of the slide rod to fit tightly against the inside of the fixed hole and fixes the slide rod inside the filter plate, thereby completing the replacement. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention.

[0019] In the attached picture:

[0020] Figure 1 The utility model is a schematic diagram of the overall structure of a natural protein purification device.

[0021] Figure 2 The utility model is a schematic diagram of the overall internal structure of a natural protein purification device.

[0022] Figure 3 A natural protein purification device of the utility model Figure 1 Schematic diagram of the structure at point A in the middle.

[0023] Figure 4 A natural protein purification device of the utility model Figure 2 Schematic diagram of the structure at point B in the middle.

[0024] Figure 5 A natural protein purification device of the utility model Figure 2 Schematic diagram of the structure at point C in the middle.

[0025] Figure 6 A natural protein purification device of the utility model Figure 1 Schematic diagram of the structure at point D in the middle.

[0026] In the figure: 1. Plug-in slot; 2. Support frame; 3. Fan gear; 4. Servo motor; 5. Circular gear; 6. Discharge pipe; 7. Controller; 8. Rotating rod; 9. Observation plate; 10. Purification box; 11. Driving motor; 12. Fixed frame; 13. Active gear; 14. Hollow rod; 15. Feeding hopper; 16. Stirring rod; 17. Drip tube; 18. Guide tube; 19. Slide rail; 20. Filter plate; 21. Solenoid valve; 22. Slide rod; 23. Limit block; 24. Spring; 25. Drag plate; 26. Fixing hole; 27. Fixing plate; 28. Purity detector; 29. ​​Driven gear; 30. Mounting plate; 31. Liquid inlet pipe. DETAILED DESCRIPTION

[0027] like Figure 3 and Figure 4 As shown, the embodiment of the utility model proposes a natural protein purification device, including a support frame 2, a purification box 10 is rotatably connected between the support frames 2, a stirring component is provided inside the purification box 10, a filtering component is provided at the bottom of the stirring component, and a swing component is provided at the bottom of the filtering component;

[0028] The stirring assembly includes a hollow rod 14 rotatably connected to the inside of the purification box 10, the top fixed sleeve of the hollow rod 14 is provided with a feeding hopper 15, the outer fixed sleeve of the hollow rod 14 is provided with a driven gear 29, the top of the purification box 10 is fixedly installed with a fixing frame 12, the top of the fixing frame 12 is fixedly installed with a driving motor 11, the output shaft of the driving motor 11 passes through one side of the fixing frame 12 and is connected with a driving gear 13, the driving gear 13 is meshed with the driven gear 29 for transmission, the outer fixed sleeve of the hollow rod 14 is provided with a plurality of drip tubes 17, and the outside of the hollow rod 14 is provided with a plurality of stirring rods 16, and the internal protein liquid and organic solvent of the purification box 10 are stirred by the stirring rods 16 outside the hollow rod 14.

[0029] like Figure 1 and Figure 5 as well as Figure 6 As shown, the filtering component includes a plug-in slot 1 opened inside the purification box 10, two slide rails 19 are provided on the side of the purification box 10 near the plug-in slot 1, and a filter plate 20 is slidably connected between the two slide rails 19. A limiting block 23 is installed on the side of the purification box 10 near the filter plate 20, and a through slot is opened inside the limiting block 23, and a spring 24 is installed inside the through slot, and a slide rod 22 is connected to one end of the spring 24, and a drag plate 25 is fixedly connected to the outside of the slide rod 22. A fixing plate 27 is installed on the outside of the filter plate 20 near the slide rod 22, and a fixing hole 26 is opened inside the fixing plate 27. The slide rod 22 passes through one side of the limiting block 23 and is movably connected to the inside of the fixing hole 26. The precipitate produced by the reaction of the protein liquid and the organic solvent is filtered through the filter plate 20 to improve the purity of the protein purification.

[0030] like Figure 1 As shown, the swing assembly includes a sector gear 3 fixedly mounted at the bottom center of the purification box 10, a servo motor 4 is mounted on the support frame 2, the output shaft of the servo motor 4 is fixedly connected to a circular gear 5, and the circular gear 5 is meshed with the sector gear 3 for transmission.

[0031] like Figure 1 and Figure 2 As shown, an observation plate 9 is provided on the outside of the purification box 10, a discharge pipe 6 is fixedly sleeved on the bottom of the purification box 10, a liquid inlet pipe 31 is fixedly sleeved on the top of the purification box 10, rotating rods 8 are fixedly installed on both sides of the purification box 10, a bearing is fixedly installed on the side of the support frame 2 close to the rotating rod 8, and the rotating rod 8 is fixedly sleeved in the bearing. The condition of the filter plate 20 can be observed through the observation plate 9. At the same time, the purification box 10 is swung in the bearing through the rotating rod 8, so that the organic solvent and the protein liquid inside the purification box 10 are fully mixed and reacted, thereby improving the purification efficiency.

[0032] like Figure 2 and Figure 5 As shown, the internal fixed sleeve of the purification box 10 is provided with a mounting plate 30, the internal fixed sleeve of the mounting plate 30 is provided with a guide tube 18, the interior of the guide tube 18 is fixedly installed with a solenoid valve 21, the outside of the purification box 10 is installed with a controller 7, the solenoid valve 21 is electrically connected to the controller 7, and the top of the mounting plate 30 is installed with a purity detector 28, which is electrically connected to the controller 7. Through the controller 7, the staff can observe the reaction between the protein liquid and the organic solvent inside the purification box 10, so as to facilitate the timely purification of the protein liquid.

[0033] Working principle:

[0034] When in use, the device is placed on a stable ground, and the plant protein liquid is purified by an organic solvent precipitation method. First, the purified protein liquid is injected into the interior of the purification box 10 through the liquid inlet pipe 31, and then the organic solvent is poured into the interior of the hollow rod 14 through the feeding hopper 15. At this time, the external power supply is turned on, so that the output shaft of the driving motor 11 drives the driving gear 13 to rotate, and then the driven gear 29 meshing with the driving gear 13 rotates, and then the hollow rod 14 is driven to rotate synchronously during the rotation process, and then under the action of centrifugation, the organic solvent is led out through the drip tube 17 outside the hollow rod 14, and the organic solvent is mixed with the extract. The protein solution inside the purification box 10 contacts, at this moment, the internal protein solution of the purification box 10 is stirred with the organic solvent by the stirring rod 16 outside the hollow rod 14, in this process, the output shaft of the servomotor 4 drives the circular gear 5 to rotate repeatedly clockwise and counterclockwise, and then the circular gear 5 drives the sector gear 3 meshing transmission, and then the purification box 10 is swung in the bearing by the rotating rod 8, and then the organic solvent inside the purification box 10 is fully mixed with the protein solution, thereby accelerating the sedimentation speed, because the purity detector 28 installed on the top of the mounting plate 30 contacts with the purified protein solution, so that the staff can observe through the controller 7 The protein solution and the organic solvent in the purification box 10 are measured to facilitate timely processing of the purification of the protein solution. After precipitation, the electromagnetic valve 21 in the guide tube 18 is opened by the controller 7, so as to perform a discharging operation on the protein solution. After the reaction, the protein solution passes through the filter plate 20, and the precipitate produced by the reaction of the protein solution and the organic solvent is filtered, and finally the purified protein solution is discharged through the drip tube 17. The situation of the filter plate 20 can be observed through the observation plate 9. When the filter plate 20 needs to be replaced, the drag plate 25 is manually moved to the left, so that the drag plate 25 drives the slide bar 22 to move to the left synchronously, so that the end of the slide bar 22 When the filter plate 20 is replaced, the filter plate 20 is inserted into the plug-in slot 1, and the plug-in slot 1 pushes the filter plate 20 inward, so that the two ends of the filter plate 20 slide along the slide rail 19 when pushed. When the fixing plate 27 contacts the end of the slide rod 22, the spring 24 contracts due to the extrusion. When the end of the slide rod 22 contacts the fixing hole 26 inside the fixing plate 27, the reaction force of the spring 24 drives the end of the slide rod 22 to fit tightly against the inside of the fixing hole 26, and fixes the slide rod 22 to the inside of the filter plate 20.

[0035] Each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the system embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiment.

[0036] The above description is only an embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included in the scope of the claims of the present invention.

Claims

1. A natural protein purification device, comprising a support frame (2), wherein a purification box (10) is rotatably connected between the support frames (2), characterized in that: The purification box (10) is provided with a stirring assembly inside, a filtering assembly is provided at the bottom of the stirring assembly, and a swinging assembly is provided at the bottom of the filtering assembly; The stirring assembly comprises a hollow rod (14) rotatably connected to the inside of a purification box (10); a feeding hopper (15) is provided on the top fixed sleeve of the hollow rod (14); a driven gear (29) is provided on the outside fixed sleeve of the hollow rod (14); a fixed frame (12) is fixedly installed on the top of the purification box (10); a driving motor (11) is fixedly installed on the top of the fixed frame (12); an output shaft of the driving motor (11) passes through one side of the fixed frame (12) and is connected to a driving gear (13); the driving gear (13) is meshed with the driven gear (29) for transmission; a plurality of dripping tubes (17) are provided on the outside of the hollow rod (14); and a plurality of stirring rods (16) are provided on the outside of the hollow rod (14).

2. A natural protein purification device according to claim 1, characterized in that: The filter assembly includes a plug-in slot (1) opened inside the purification box (10), two slide rails (19) are provided on the side of the purification box (10) close to the plug-in slot (1), and a filter plate (20) is slidably connected between the two slide rails (19). A limit block (23) is installed on the side of the purification box (10) close to the filter plate (20), a through slot is opened inside the limit block (23), a spring (24) is installed inside the through slot, one end of the spring (24) is connected to a slide rod (22), the outside of the slide rod (22) is fixedly connected to a drag plate (25), a fixing plate (27) is installed on the outside of the filter plate (20) close to the slide rod (22), a fixing hole (26) is opened inside the fixing plate (27), and the slide rod (22) passes through one side of the limit block (23) and is movably connected to the inside of the fixing hole (26).

3. A natural protein purification device according to claim 1, characterized in that: The swing assembly comprises a sector gear (3) fixedly mounted at the bottom center of the purification box (10); a servo motor (4) is mounted on the support frame (2); an output shaft of the servo motor (4) is fixedly connected to a circular gear (5); and the circular gear (5) is meshed with the sector gear (3) for transmission.

4. A natural protein purification device according to claim 1, characterized in that: An observation panel (9) is provided on the outside of the purification box (10), a discharge pipe (6) is provided on the bottom fixed sleeve of the purification box (10), and a liquid inlet pipe (31) is provided on the top fixed sleeve of the purification box (10).

5. A natural protein purification device according to claim 1, characterized in that: The purification box (10) is provided with a mounting plate (30) in a fixed sleeve inside, the mounting plate (30) is provided with a flow guide tube (18) in a fixed sleeve inside, a solenoid valve (21) is fixedly installed inside the flow guide tube (18), a controller (7) is installed outside the purification box (10), and the solenoid valve (21) is electrically connected to the controller (7).

6. A natural protein purification device according to claim 5, characterized in that: A purity detector (28) is installed on the top of the mounting plate (30), and the purity detector (28) is electrically connected to the controller (7).

7. A natural protein purification device according to claim 1, characterized in that: Rotating rods (8) are fixedly mounted on both sides of the purification box (10), and a bearing is fixedly mounted on one side of the support frame (2) close to the rotating rod (8), and the rotating rod (8) is fixedly sleeved in the bearing.

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