Multifunctional single-directional immunodiffusion experiment operation device

By designing a multi-functional single-direction immune diffusion experiment operating device, automatic leveling, constant temperature and moisturizing control are achieved, which solves the problem of poor experimental environment stability in single-direction immune diffusion experiments, and improves the accuracy and reliability of experimental results.

CN222739219UActive Publication Date: 2025-04-11DALIAN ALEPH BIOMEDICAL
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Patent Information

Application Number
CN202421349538.9
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-06-13
Publication Date
2025-04-11
Estimated Expiration
2034-06-13

AI Technical Summary

Technical Problem

During the single-to-immuno-diffusion experiment, the leveling operation of the agarose gel reaction plate relies on manual adjustment, which takes a long time and is not ideal, and the antigen-antibody reaction environment is difficult to maintain in an ideal state for a long time.

Method used

A multi-functional single-directional immune diffusion experiment operation device is designed, including a constant temperature horizontal static table, a heating insulation rack and an electric lifting device, equipped with a tiltmeter, a moisturizing system and a PLC control system to realize automatic leveling, constant temperature, moisturizing and regular water change functions.

Benefits of technology

Through automatic leveling and constant temperature control, the stability and reliability of the experimental environment are improved, the operation complexity and manual error are reduced, and the accuracy of the experimental results are ensured.

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Abstract

The utility model relates to a multifunctional single-direction immunodiffusion experiment operation device, including a constant temperature horizontal station, a heating and heat preservation rack and an electric lifting device, the constant temperature horizontal station and the heating and heat preservation rack are the same frame, a certain distance is maintained between the two, and the two are blocked by a heat insulation board, the four corners of the frame of the constant temperature horizontal station and the heating and heat preservation rack are respectively fixed on the corresponding electric lifting device, the position of the lower center of the frame of the constant temperature horizontal station and the heating and heat preservation rack is installed with an inclinometer, and the inclinometer is used to monitor the inclination angle of the frame; the interior of the constant temperature horizontal station is a water cavity, the bottom of the constant temperature horizontal station is provided with a water changing system, an electric heater is installed in the water cavity inside the constant temperature horizontal station, the upper surface of the constant temperature horizontal station is a horizontal operating table with raised edges around, and the table is a static area of ​​an agarose gel plate. The utility model saves the operation and waiting time of the staff, reduces the labor intensity of the staff, and improves work efficiency.
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Description

Technical Field

[0001] The utility model relates to the technical field of quality inspection or scientific research experimental equipment, and more specifically, to a multifunctional single-direction immunodiffusion experiment operating device that provides functions such as automatic leveling, automatic temperature adjustment and moisturizing, timed water replacement, and automatic alarm during the inspection or experimental process. The operation process of the single-direction immunodiffusion experiment is extremely important. Background Art

[0002] With the rapid development of automation and intelligence, some quality inspection or scientific research equipment has been in manual mode. There may be a lot of products and achievements, but these basic experimental facilities are still stagnant in the previous state, especially in key and easily overlooked places such as production quality inspection or scientific research laboratories. In the past, the leveling operation of the agarose gel reaction plate during the test of hemagglutinin content in influenza virus split vaccine (single-directional immune diffusion experiment) could only rely on manual adjustment. The process was complicated and time-consuming, and the leveling effect was often not ideal. In addition, the antigen-antibody reaction is placed at room temperature, and the room space is large, so it is difficult to maintain it in an ideal state for a long time. Therefore, it is difficult to guarantee the leveling and experimental environment during the experiment. Utility Model Content

[0003] According to the technical problems raised above, a multifunctional single-directional immunodiffusion experiment operation device is provided.

[0004] The technical means adopted by the utility model are as follows:

[0005] A multifunctional single-directional immunodiffusion experiment operation device, comprising a constant temperature horizontal station, a heating and heat preservation rack and an electric lifting device, wherein the constant temperature horizontal station and the heating and heat preservation rack are of the same frame, a certain distance is maintained between the two, and a heat insulation board is used to block the two, so that the temperatures of the constant temperature horizontal station and the heating and heat preservation rack are different and do not affect each other, the four corners of the frames of the constant temperature horizontal station and the heating and heat preservation rack are respectively fixed on the corresponding electric lifting devices, and an inclinometer is installed at the center of the lower part of the frames of the constant temperature horizontal station and the heating and heat preservation rack, and the inclinometer is used to monitor the inclination angle of the frame;

[0006] The interior of the constant temperature horizontal station is a water cavity, a water changing system is arranged at the bottom of the constant temperature horizontal station, an electric heater is installed in the water cavity inside the constant temperature horizontal station, the upper surface of the constant temperature horizontal station is a horizontal operating table with raised edges around, and the table is a static area for the agarose gel plate;

[0007] The interior of the heating and insulation rack is a water cavity, a water inlet system is arranged at the bottom of the heating and insulation rack, an electric heater is installed in the water cavity inside the heating and insulation rack, the upper surface of the heating and insulation rack is a groove for accommodating a plurality of conical flasks, and a heat preservation cover is arranged on the upper part of the groove.

[0008] Furthermore, the electric lifting device includes a micro transmission motor, a base bracket, an externally threaded rotating pillar, a movable sleeve and a column with a central internal thread. The outer shell of the micro transmission motor is fixed to the top of the movable sleeve through the base bracket. The rotating shaft of the micro transmission motor is connected to the externally threaded rotating pillar, so that the externally threaded rotating pillar rotates with the rotating shaft of the micro transmission motor. The lower end of the externally threaded rotating pillar passes through the movable sleeve and is threadedly connected to the column with the central internal thread. The lower end of the movable sleeve is sleeved on the upper end of the column with the central internal thread, which is in natural sliding contact; the movable sleeve is respectively connected to the frame corners of the constant temperature horizontal stationary platform and the heating and insulation rack through axial holes.

[0009] Furthermore, the upper surface of the constant temperature horizontal station is surrounded by raised edges and has a moisturizing open water ditch underneath, and a moisturizing system is provided at its upper left corner. The moisturizing system includes an exposed float, a soft steel wire is connected under the float, and a valve with a reset spring is connected to the lower end of the soft steel wire. When there is no water or little water in the moisturizing open water ditch, the float of the moisturizing system moves downward, the valve with the reset spring opens, water enters the moisturizing open water ditch, the float of the moisturizing system moves upward, and the valve with the reset spring closes.

[0010] Furthermore, the lower part of the groove for heating the conical flask of the heating and insulation rack is connected to the water cavity, the water level is 20 to 50 mm higher than the bottom of the groove, and an overflow pipe is provided on the corresponding rear wall in the upper middle part of the groove.

[0011] Furthermore, it also includes a PLC system, the inclinometer is connected to the PLC system, and each electric lifting device is connected to the PLC system. The inclinometer outputs an electrical signal to the PLC system in the control box, and the angles in the three directions of X / Y / Z are displayed on the human-machine interface after being processed by the PLC; the micro-transmission motor is connected to the electrical signal of the PLC system in the control box, and can be displayed and controlled on the human-machine interface after being processed by the PLC; the control box controls the rotation of the micro-transmission motor, and the horizontal adjustment of the multifunctional single-directional immune diffusion experiment operating device is achieved through the forward and reverse rotation of each micro-transmission motor. Based on the real-time angle detection of the three directions of X / Y / Z by the inclinometer, the PLC system controls the corresponding micro-transmission motor to complete the leveling.

[0012] Furthermore, a water inlet solenoid valve and a water discharge solenoid valve are respectively provided on the water inlet pipe and the water outlet pipe of the constant temperature horizontal still platform, and the water inlet solenoid valve and the water discharge solenoid valve are connected to the PLC system electrical signal in the control box; a thermal resistor is installed on the side wall of the water cavity inside the constant temperature horizontal still platform for real-time detection of the water temperature inside the constant temperature horizontal still platform, and the thermal resistor and the electric heater are respectively connected to the PLC system electrical signal in the control box;

[0013] A water inlet solenoid valve is provided on the water inlet pipe of the heating and insulation rack, and the water inlet solenoid valve is connected to the electrical signal of the PLC system in the control box; a thermal resistor is installed on the side wall of the water cavity inside the heating and insulation rack for real-time detection of the water temperature inside it, and the thermal resistor and the electric heater are respectively connected to the electrical signal of the PLC system in the control box.

[0014] Furthermore, the control panel of the control box includes a monitoring interface, control buttons, a timing module and an alarm module. The control buttons are connected to the execution unit of the PLC system. The monitoring interface displays the operating status of the constant temperature horizontal still table and the heating and insulation rack. The control buttons include control modules of each electric lifting device, a constant temperature horizontal still table heating and water control module, and a heating and water control module of the heating and insulation rack. Each control button includes an automatic control module and a manual control module. The alarm module is provided with an alarm query interface and an operation alarm interface; the alarm query interface is used to view alarm information and delete alarm records; the operation alarm interface is automatically popped up and displays alarm information; the timing module is used to obtain time and interact with the PLC system to complete the opening and closing of each functional module within a preset time period under automatic control.

[0015] Compared with the prior art, the utility model has the following advantages:

[0016] The constant temperature horizontal station and the heating and insulation rack are observed and adjusted in the same frame, eliminating the need to operate different experiments in different devices and reducing the complexity of the operator's operation. The two are separated by a heat insulation board to ensure that the temperature between the constant temperature horizontal station and the heating and insulation rack is different and does not affect each other. The inclinometer installed at the center of the lower part of the frame of the constant temperature horizontal station and the heating and insulation rack is convenient for the operator to observe the tilt of the entire frame. The operator can perform precise leveling through the electric lifting device to ensure that the horizontal situation of the multifunctional single-directional immune diffusion experiment operation device always meets the experimental requirements. The moisturizing system can ensure that there is always water in the moisturizing open water ditch under the raised edge around the constant temperature horizontal station, which can moisturize the agarose gel plate on the constant temperature horizontal station through evaporation. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0018] Figure 1 It is a side view of the multifunctional unidirectional immunodiffusion experiment operation device.

[0019] Figure 2 This is a top view of the multifunctional single-directional immunodiffusion experiment operating device.

[0020] Figure 3 for Figure 1 AA view of the .

[0021] Figure 4 for Figure 2 BB view.

[0022] Figure 5 This is an enlarged view of the control panel of the utility model.

[0023] Figure 6 This is a control logic diagram of Example 2 of the utility model.

[0024] In the figure: 1. Electric lifting device; 2. Constant temperature horizontal station; 3. Water inlet solenoid valve; 4. Drain solenoid valve; 5. Inclinometer; 6. Agarose gel plate; 7. Thermal resistor; 8. Electric heater; 9. Water; 10. Insulation board; 11. Erlenmeyer flask; 12. Insulation cover; 13. Human-machine interface; 14. Control box; 15. Heating and insulation rack; 16. Overflow pipe; 17. Moisturizing system; 1-1. Micro transmission motor; 1-2. Base bracket; 1-3. External thread rotating pillar; 1-4. Moving sleeve; 1-5. Column with central internal thread. DETAILED DESCRIPTION

[0025] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments of the present invention can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0026] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means a limitation on the utility model and its application or use. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0027] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the utility model. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0028] Unless otherwise specifically stated, the relative arrangement of the parts and steps described in these embodiments, the numerical expressions and numerical values ​​do not limit the scope of the utility model. At the same time, it should be clear that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to ordinary technicians in the relevant field may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be regarded as part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further discussed in subsequent drawings.

[0029] In the description of the present utility model, it needs to be understood that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "lateral, vertical, perpendicular, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the drawings. They are only for the convenience of describing the present utility model and simplifying the description. Unless otherwise stated, these directional words do not indicate or imply that the device or element referred to must have a specific direction or be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the scope of protection of the present utility model: the directional words "inside and outside" refer to the inside and outside relative to the contours of each component itself.

[0030] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below their position devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.

[0031] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. If not otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of the utility model.

[0032] Example 1

[0033] like Figures 1 to 4 As shown, an embodiment of the utility model discloses a multifunctional single-directional immune diffusion experiment operation device, including a constant temperature horizontal stationary platform 2, a heating and heat preservation rack 15 and an electric lifting device 1. The constant temperature horizontal stationary platform 2 and the heating and heat preservation rack 15 are the same frame, and a certain distance is maintained between the two. The two are blocked by a heat insulation board 10. In this embodiment, the two are kept at a distance of about 100 mm, so that the temperatures between the constant temperature horizontal stationary platform 2 and the heating and heat preservation rack 15 are different and do not affect each other. The four corners of the frames of the constant temperature horizontal stationary platform 2 and the heating and heat preservation rack 15 are respectively fixed on the corresponding electric lifting device 1, and an inclinometer 5 is installed at the center of the lower part of the frames of the constant temperature horizontal stationary platform 2 and the heating and heat preservation rack 15. The inclinometer 5 is used to monitor the inclination angle of the frame; the inclinometer used in this embodiment can be a prior art.

[0034] The interior of the constant temperature horizontal station 2 is a water cavity, a water changing system is provided at the bottom of the constant temperature horizontal station 2, an electric heater 8 is installed in the water cavity inside the constant temperature horizontal station 2, and the upper surface of the constant temperature horizontal station is a horizontal operating table with raised edges around, and the table is a static area for the agarose gel plate 6;

[0035] Different from the constant temperature horizontal still table 2, at the height corresponding to the operating table of the constant temperature horizontal still table 2, the inside of the heating and insulation rack is still a water cavity, and above it is a groove for heating the conical flask 11. A water inlet system is provided at the bottom of the heating and insulation rack, and an electric heater 8 is installed in the water cavity inside the heating and insulation rack. The upper surface of the heating and insulation rack is a groove for accommodating a number of conical flasks 11, and a heat preservation cover 12 is provided on the upper part of the groove. The heat preservation cover is reversible. When the conical flask 11 is placed in the groove of the heating and insulation rack 15, the heat preservation cover 12 is covered, so that the conical flask 11 can be quickly heated, and the temperature of the space where the temperature is located is closer to the temperature of the heating medium water 9.

[0036] As a specific implementation, in this embodiment, the electric lifting device 1 includes a micro transmission motor 1-1, a base bracket 1-2, an externally threaded rotating pillar 1-3, a movable sleeve 1-4 and a column 1-5 with a central internal thread. The shell of the micro transmission motor 1-1 is fixed to the top of the movable sleeve 1-4 through the base bracket 1-2, and the rotating shaft of the micro transmission motor 1-1 is connected to the externally threaded rotating pillar 1-3, so that the externally threaded rotating pillar 1-3 rotates with the rotating shaft of the micro transmission motor 1-1. The lower end of the externally threaded rotating pillar 1-3 passes through the movable sleeve 1-4 and is threadedly connected to the column 1-5 with a central internal thread. The lower end of the movable sleeve 1-4 is sleeved on the upper end of the column 1-5 with a central internal thread, and there is a natural sliding contact between the two; the movable sleeve 1-4 is respectively connected to the frame corners of the constant temperature horizontal stationary platform 2 and the heating and insulation rack 15 through axial holes.

[0037] The upper surface of the constant temperature horizontal station is surrounded by raised edges with a moisturizing open water ditch at the bottom, and a moisturizing system 17 is provided at its upper left corner. The moisturizing system 17 includes an exposed float, a soft steel wire is connected under the float, and a valve with a reset spring is connected to the lower end of the soft steel wire. When there is no water or little water in the moisturizing open water ditch, the float of the moisturizing system 17 moves downward, the valve with the reset spring opens, water enters the moisturizing open water ditch, the float of the moisturizing system 17 moves upward, and the valve with the reset spring closes.

[0038] The lower part of the groove heated by the conical flask of the heating and insulation rack is connected to the water cavity, and the water level is 20-50 mm higher than the bottom of the groove. An overflow pipe 16 is provided on the corresponding rear wall of the upper middle part of the groove, and the outer end of the overflow pipe 16 is connected to the lower water.

[0039] Through the above implementation, technicians in this field can effectively observe the experimental environment of the constant temperature horizontal station and the heating and insulation rack and manually adjust the relevant mechanisms of the experimental environment based on the observation results, thereby ensuring the reliability of the experiment.

[0040] Example 2

[0041] like Figure 5As shown, as a preferred embodiment, a PLC system arranged in a control box 14 can also be included to complete the automatic leveling, temperature control and other operations of the device. In this embodiment, the inclinometer 5 is connected to the PLC system, and each electric lifting device 1 is connected to the PLC system. The inclinometer 5 outputs an electrical signal to the PLC system in the control box 14, and the angles in the three directions of X / Y / Z are displayed on the human-machine interface 13 after being processed by the PLC; the micro-transmission motor 1-1 is connected to the PLC system electrical signal in the control box, and can be displayed and controlled on the human-machine interface after being processed by the PLC; the control box controls the rotation of the micro-transmission motor 1-1, and the horizontal adjustment of the multifunctional single-direction immune diffusion experiment operating device is realized through the forward and reverse rotation of each micro-transmission motor 1-1. Based on the real-time angle detection of the three directions of X / Y / Z by the inclinometer 5, the four corners of the frame can rise or fall respectively with the rise and fall of the corresponding electric lifting device 1, and the PLC system controls the corresponding micro-transmission motor 1-1 to complete the leveling.

[0042] Specifically, the control box 14 controls the rotation of the micro transmission motor 1-1, that is, controls the rotation of the external thread rotating support 1-3. With the rotation of the external thread rotating support 1-3, the movable sleeve 1-4 can be driven to move up and down along the axis of the column 1-5 with the central internal thread. The horizontal adjustment of the multifunctional single-directional immune diffusion experiment operating device is achieved through the forward and reverse rotation of the four micro transmission motors 1-1. In addition, the inclinometer 5 detects the angles in the three directions of X / Y / Z in real time, so that fully automatic closed-loop control can be achieved.

[0043] Specifically, the water inlet and outlet pipes of the constant temperature horizontal station are respectively provided with a water inlet solenoid valve 3 and a water discharge solenoid valve 4, and the water inlet solenoid valve 3 and the water discharge solenoid valve 4 are connected to the PLC system electrical signal in the control box 14; a thermal resistor 7 is installed on the side wall of the water cavity inside the constant temperature horizontal station 2 for real-time detection of the water temperature inside the constant temperature horizontal station 2, and the thermal resistor 7 and the electric heater 8 are respectively connected to the PLC system electrical signal in the control box 14;

[0044] A water inlet solenoid valve 3 is provided on the water inlet pipe of the heating and insulation rack 15, and the water inlet solenoid valve 3 is connected to the electrical signal of the PLC system in the control box; a thermal resistor 7 is installed on the side wall of the water chamber inside the heating and insulation rack 15, which is used to perform real-time detection of the temperature of the water 9 inside it, and the thermal resistor 7 and the electric heater 8 are respectively connected to the electrical signal of the PLC system in the control box 14.

[0045] The water inlet solenoid valve 3 and the water discharge solenoid valve 4 are opened and closed by PLC to realize water storage 9 and water change 9 inside the constant temperature horizontal station 2; after PLC processing, the temperature can be displayed on the human-machine interface 13 and the heater can be controlled to start and shut down.

[0046] Based on the above equipment, the utility model includes four functions. First, the angle of the device in the three directions of X / Y / Z is constantly monitored by the inclinometer 5. If tilt occurs, it can be automatically leveled and the occurrence time, duration and tilt angle of the tilt can be recorded; secondly, the temperature of the constant temperature horizontal station 2 is constant by controlling the heating system; thirdly, the temperature in the heating and insulation rack 15 is maintained within the temperature range required by the working of the conical flask 11 by controlling the heater. Finally, the moisturizing system 17 can humidify the antigen-antibody reaction environment to ensure a certain humidity and prevent the gel from drying up. The utility model can monitor and control the horizontality of the single immunodiffusion test operating device and the temperature and humidity required by each functional area in real time and accurately. The agarose gel on the agarose gel plate 6 is not evenly spread or tilted during the reaction process due to the uneven adjustment of the manual operation table or external disturbance during the static process, which affects the experimental effect; the constant temperature horizontal static table 2 and the heating and insulation rack 15 are equipped with an automatic control system to ensure more stable temperature, and have an alarm function to actively remind the staff of the time, reduce the staff's intensity and error rate, greatly improve the stability of various parameters in the experimental process, and provide a strong guarantee for the accuracy of the experimental results.

[0047] like Figure 6 As shown, △X is the deviation value between the X-axis and the horizontal state, and △Y is the deviation value between the Y-axis and the horizontal state, wherein the PLC system presets the inclination angle, which is 0.2° in this embodiment, and 0.2° is the assumed allowable deviation value, which can be set according to demand. When the inclinometer detects that the inclination angle of one of the frames exceeds the preset value, the PLC system controls the corresponding electric lifting device to move and adjust it to the preset inclination angle. In fact, the forward and reverse drives are synchronized during the adjustment process, and each time the electric lifting device is adjusted, the running time of the corresponding electric lifting device is determined according to the size of the deviation value. The angle adjusted by the electric lifting device each time is very small. The data is updated in real time through the inclinometer, and the PLC system continuously judges the new data and controls the corresponding electric lifting device until the data detected by the inclination detector meets the angle required by the multifunctional single-directional immune diffusion experiment operating device, and the leveling system enters a stable state.

[0048] The specific implementation method of Example 2 is as follows: the multifunctional single-directional immune diffusion experiment operation device is set for experimental requirements through the human-machine interface 13. For example, the experiment starts at 9 o'clock every day, the temperature of the constant temperature horizontal station 2 is controlled at 21.0±2.0℃, the temperature of the heating and insulation rack 15 is controlled at 50.0±1.0℃, the horizontal accuracy is maintained at 0.1±0.01°, the insulation time of the conical flask 11 is 60 minutes, and the agarose gel plate 6 is left to stand for 22 hours. After the requirements are met, press the automatic operation button, and the multifunctional single-directional immune diffusion experiment operation device starts to work. First, at 8:30 (the device will automatically adjust the time in advance based on the previous three working times), the multifunctional single-directional immune diffusion experiment operation device is self-checked. After the inspection is completed, it is leveled. After the leveling is completed, it enters the background and keeps running. The inclinometer 5 is monitored online in real time. When the deviation angle in a certain direction is greater than the setting requirement, one or two micro-drive motors 1-1 are forwarded or reversed according to the deviation angles in the three directions, so that the horizontal degree of the multifunctional single-directional immune diffusion experiment operation device is within the requirement. Then the water in the constant temperature horizontal station table 2 and the heating and insulation rack 15 is changed, and the PLC automatically controls the water inlet solenoid valve 3 and the drain solenoid valve 4 to complete the automatic water change (the water inlet pipe and the drain pipe are respectively connected to the water supply pipe and the drain pipe). The heating and insulation rack 15 does not have a drain solenoid valve 4, but has an overflow pipe, which can also be achieved; after the water is changed, the constant temperature horizontal station table 2 and the heating and insulation rack 15 are heated respectively, and the heating is automatically stopped when the temperature reaches the setting requirement, and both enter the constant temperature insulation stage; when the time reaches nine o'clock, there will be a buzzer to remind that the experiment time is up, and the heating is controlled according to the temperature detected in real time by the corresponding thermal resistor 7. The staff puts the boiling conical flask 11 with agarose gel into the heating and insulation rack 15, keeps the conical flask 11 warm, and takes out the agarose gel according to the experimental needs and introduces it into the agarose gel plate 6. When the agarose gel plate is placed on the constant temperature horizontal station 2 after adding the sample and the standard, it will also realize automatic temperature control according to the temperature. When the static time is up, a buzzer will be automatically emitted to remind the staff that the static time has come. At the same time, during the static process, the moisturizing system 17 in the constant temperature horizontal station 2 can always ensure that there is water in the moisturizing open water ditch, so as to achieve the purpose of moisturizing. When the multifunctional single-direction immune diffusion experiment operation device completes all the work, it will enter the automatic standby state until the next experiment before entering the cycle. This device can also enter the manual state, and the staff can operate while observing data and phenomena.

[0049] It should be understood that in Example 2 of the present application, no improvement is made to the program, and the control programs involved in the present application are all prior arts.

[0050] The advantages of the utility model mainly include:

[0051] First, the inclinometer constantly monitors the angle of the device in the X / Y / Z directions. If tilt occurs, it can be automatically leveled and can record the time, duration, and angle of tilt. Secondly, the constant temperature of the constant temperature horizontal table can be achieved by controlling the heating system. Furthermore, the heater is controlled to ensure that the temperature in the heating and insulation rack is maintained within the temperature range required for the working of the conical flask. Finally, the moisturizing system can humidify the antigen-antibody reaction environment to ensure a certain humidity and prevent the gel from drying up.

[0052] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the utility model.

Claims

1. A multifunctional single-directional immunodiffusion experiment operating device, characterized in that: It includes a constant temperature horizontal station, a heating and heat preservation rack and an electric lifting device. The constant temperature horizontal station and the heating and heat preservation rack are in the same frame. A certain distance is maintained between the two. The two are separated by a heat insulation board so that the temperatures of the constant temperature horizontal station and the heating and heat preservation rack are different and do not affect each other. The four corners of the frames of the constant temperature horizontal station and the heating and heat preservation rack are respectively fixed on the corresponding electric lifting devices. An inclinometer is installed at the center of the lower part of the frames of the constant temperature horizontal station and the heating and heat preservation rack. The inclinometer is used to monitor the inclination angle of the frame; The interior of the constant temperature horizontal station is a water cavity, a water changing system is arranged at the bottom of the constant temperature horizontal station, an electric heater is installed in the water cavity inside the constant temperature horizontal station, the upper surface of the constant temperature horizontal station is a horizontal operating table with raised edges around, and the table is a static area for the agarose gel plate; The interior of the heating and insulation rack is a water cavity, a water inlet system is arranged at the bottom of the heating and insulation rack, an electric heater is installed in the water cavity inside the heating and insulation rack, the upper surface of the heating and insulation rack is a groove for accommodating a plurality of conical flasks, and a heat preservation cover is arranged on the upper part of the groove.

2. The multifunctional single-directional immunodiffusion experiment operation device according to claim 1 is characterized in that: The electric lifting device includes a micro transmission motor, a base bracket, an external thread rotating pillar, a movable sleeve and a column with a central internal thread. The shell of the micro transmission motor is fixed to the top of the movable sleeve through the base bracket. The rotating shaft of the micro transmission motor is connected to the external thread rotating pillar, so that the external thread rotating pillar rotates with the rotating shaft of the micro transmission motor. The lower end of the external thread rotating pillar passes through the movable sleeve and is threadedly connected to the column with the central internal thread. The lower end of the movable sleeve is sleeved on the upper end of the column with the central internal thread, which is in natural sliding contact. The movable sleeve is respectively connected to the frame corners of the constant temperature horizontal stationary platform and the heating and insulation rack through shaft holes.

3. The multifunctional single-directional immunodiffusion experiment operation device according to claim 1 is characterized in that: The upper surface of the constant temperature horizontal station is surrounded by raised edges and has a moisturizing open water ditch underneath. A moisturizing system is provided at its upper left corner. The moisturizing system includes an exposed float, a soft steel wire is connected under the float, and a valve with a reset spring is connected to the lower end of the soft steel wire. When there is no water or little water in the moisturizing open water ditch, the float of the moisturizing system moves downward, the valve with the reset spring opens, water enters the moisturizing open water ditch, the float of the moisturizing system moves upward, and the valve with the reset spring closes.

4. The multifunctional single-directional immunodiffusion experiment operation device according to claim 1, characterized in that: The lower part of the groove heated by the conical flask of the heating and insulation rack is connected to the water cavity, the water level is 20-50mm higher than the bottom of the groove, and an overflow pipe is arranged on the corresponding rear wall of the upper middle part of the groove.

5. The multifunctional unidirectional immunodiffusion experiment operation device according to any one of claims 1 to 4, characterized in that: The invention also includes a PLC system, an inclinometer is connected to the PLC system, and each electric lifting device is connected to the PLC system. The inclinometer outputs an electrical signal to the PLC system in the control box, and the angles in the three directions of X / Y / Z are displayed on the human-machine interface after being processed by the PLC; the micro transmission motor is connected to the electrical signal of the PLC system in the control box, and the angles in the three directions of X / Y / Z are displayed and controlled on the human-machine interface after being processed by the PLC; the control box controls the rotation of the micro transmission motor, and the horizontal adjustment of the multifunctional single-directional immune diffusion experiment operating device is realized through the forward and reverse rotation of each micro transmission motor. Based on the real-time angle detection of the three directions of X / Y / Z by the inclinometer, the PLC system controls the corresponding micro transmission motor to complete the leveling.

6. The multifunctional unidirectional immunodiffusion experiment operation device according to claim 5, characterized in that: The water inlet and outlet pipes of the constant temperature horizontal still platform are respectively provided with a water inlet solenoid valve and a water discharge solenoid valve, which are connected to the PLC system electrical signal in the control box; a thermal resistor is installed on the side wall of the water cavity inside the constant temperature horizontal still platform for real-time detection of the water temperature inside the constant temperature horizontal still platform, and the thermal resistor and the electric heater are respectively connected to the PLC system electrical signal in the control box; A water inlet solenoid valve is provided on the water inlet pipe of the heating and insulation rack, and the water inlet solenoid valve is connected to the electrical signal of the PLC system in the control box; a thermal resistor is installed on the side wall of the water cavity inside the heating and insulation rack for real-time detection of the water temperature inside it, and the thermal resistor and the electric heater are respectively connected to the electrical signal of the PLC system in the control box.