A device for isolating intestinal crypt stem cells

Abstract

The utility model relates to the technical field of stem cell extraction and separation, and discloses an intestinal crypt stem cell separation device. The utility model provides such an intestinal crypt stem cell separation device, including an installation box, a hinge, a torsion spring, a flap, a servo motor, a rotating frame and a guide block. Two hinges are installed on the installation box, and the two hinges are rotatably provided with a flap, and two torsion springs are sleeved on the hinge. A servo motor is installed inside the installation box, and a rotating frame is connected to the output shaft of the servo motor. The rotating frame is provided with multiple guide grooves, and guide blocks are slidably provided inside the guide grooves. A screw is also included, and multiple screws are rotatably provided on the rotating frame, and the screws are threadedly connected to the guide blocks. The guide blocks are driven to move by rotating the screws, and the distance between the charging box and the center point of the rotating frame is adjusted, and then the centrifugal radius of the device is adjusted, so as to facilitate the adjustment of the magnitude of the centrifugal force, so as to meet different research needs.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell extraction and separation, and discloses an intestinal crypt stem cell separation device. Background Art

[0002] Intestinal stem cells are located at the base of the intestinal mucosal crypts, that is, the basal crypts are the cell bank of intestinal stem cells. Under normal circumstances, intestinal stem cells located at the base of the crypts continue to migrate toward the top of the crypts (toward the intestinal cavity). The entire migration process takes about 3 to 5 days. During the migration process, intestinal stem cells differentiate into different intestinal mucosal cells. Cell centrifugation is an indispensable method in cell research and cell culture.

[0003] The patent authorization publication number is CN216445346U, which discloses a stem cell separation device, including a base and a support tube arranged on the base, a connecting assembly, a plurality of rotating rods are rotatably arranged on the support tube, and the end of the rotating rod away from the support tube is connected to a rotating plate through a moving assembly, and a placement tube is arranged on the rotating rod. Although the above patent can drive the clamping block to move in the placement tube through the moving block so that the clamping block can clamp the test tube in the placement tube, because the placement tube is arranged on the rotating rod, it is impossible to change the distance between the center point of the placement tube and the support tube by adjusting the position of the placement tube, thereby failing to adjust the magnitude of the centrifugal force, and failing to meet different research needs.

[0004] Therefore, there is a need for an intestinal crypt stem cell separation device that is easy to adjust the centrifugal force. Utility Model Content

[0005] In order to overcome the shortcomings of the existing patent that the placement tube is set on the rotating rod, and it is impossible to change the distance between the placement tube and the center point of the support tube by adjusting the position of the placement tube, thereby failing to adjust the size of the centrifugal force and thus failing to meet different research needs, the utility model provides an intestinal crypt stem cell separation device that is easy to adjust the centrifugal force.

[0006] The utility model provides the following technical scheme: an intestinal crypt stem cell separation device, comprising an installation box, a hinge, a torsion spring, a flap, a servo motor, a rotating frame, a loading box, a guide block and a clamping mechanism, wherein two hinges are installed on the installation box, the two hinges are rotatably provided with a flap, two torsion springs are sleeved on the hinges, a servo motor is installed inside the installation box, a rotating frame is connected to the output shaft of the servo motor, a plurality of guide grooves are opened on the rotating frame, guide blocks are slidably provided inside the guide grooves, a loading box is fixedly connected to the guide blocks, a clamping mechanism is provided inside the loading box, and also comprises a screw, a plurality of screws are rotatably provided on the rotating frame, and the screws are threadedly connected to the guide blocks.

[0007] As a preferred technical solution of the utility model, the clamping mechanism includes a first clamping plate, a second clamping plate, a guide rod and a first elastic member. The first clamping plate is fixedly connected to the inside of the loading box, a guide rod is slidably arranged on the loading box, the second clamping plate is fixedly connected to the guide rod, and the first elastic member is sleeved on the guide rod.

[0008] As a preferred technical solution of the utility model, it also includes a mounting block, a second elastic member, a clamping block and a guide clamping ring. Two mounting blocks are fixedly connected to the mounting box. A clamping groove is opened on the mounting block. A clamping block is slidably arranged inside the clamping groove. A second elastic member is connected between the clamping block and the mounting block. A guide clamping ring is fixedly connected inside the flip cover.

[0009] As a preferred technical solution of the utility model, it also includes a sealing ring, and the sealing ring is arranged on the guide clamping ring.

[0010] As a preferred technical solution of the utility model, it also includes a water-blocking plate, and the bottom of the installation box is fixedly connected with the water-blocking plate.

[0011] As a preferred technical solution of the utility model, it also includes a rubber pad, and the rubber pad is embedded on the inner wall of the second clamping plate.

[0012] Compared with the prior art, the utility model provides an intestinal crypt stem cell separation device with the following beneficial effects: 1. By rotating the screw to drive the guide block to move, the distance between the loading box and the center point of the rotating frame is adjusted, and then the centrifugal radius of the device is adjusted, so as to facilitate the adjustment of the centrifugal force to meet different research needs.

[0013] 2. When the flip cover is no longer squeezing the card block, the second elastic member returns to its original state to move the card block forward and reset it to engage with the flip cover, thereby limiting the flip cover and preventing the flip cover from rotating upward. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a three-dimensional structural schematic diagram of the utility model.

[0015] Figure 2 It is a three-dimensional cross-sectional structural schematic diagram of the servo motor and the rotating frame of the utility model.

[0016] Figure 3 It is a three-dimensional cross-sectional structural schematic diagram of the screw rod, the charging box and the first clamping plate of the utility model.

[0017] Figure 4 It is a three-dimensional cross-sectional structural schematic diagram of the flip cover and the mounting block of the utility model.

[0018] Figure 5 For this utility model Figure 4 Enlarged view of point A in the middle.

[0019] Figure 6 It is a three-dimensional cross-sectional structural schematic diagram of the clamping block of the utility model.

[0020] Figure 7 It is a three-dimensional cross-sectional structural schematic diagram of the guide clamp ring and the sealing ring of the utility model.

[0021] Figure 8 It is a three-dimensional structural schematic diagram of the utility model water-blocking board.

[0022] Among them: 1-installation box, 2-hinge, 201-torsion spring, 3-flip cover, 4-servo motor, 5-rotating frame, 501-guide groove, 6-screw, 7-loading box, 701-guide block, 8-first clamping plate, 9-second clamping plate, 10-guide rod, 11-first elastic member, 12-installation block, 13-slot, 14-second elastic member, 15-block, 16-guide clamping ring, 1601-sealing ring, 17-waterproof plate, 18-rubber pad. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Example 1: A device for isolating intestinal crypt stem cells, see Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 7 and Figure 8As shown, it includes an installation box 1, a hinge 2, a torsion spring 201, a flap 3, a servo motor 4, a rotating frame 5, a charging box 7, a guide block 701 and a clamping mechanism. Two hinges 2 are provided on the installation box 1 by means of bolt connection. The two hinges 2 are provided with a flap 3 for rotation together. Two torsion springs 201 are sleeved on the hinge 2. Both ends of the torsion spring 201 are respectively connected to the hinge 2. A servo motor 4 is provided inside the installation box 1 by means of bolt connection. The output shaft of the servo motor 4 is connected to the rotating frame 5. Three guide grooves 501 are opened on the rotating frame 5. The guide grooves 501 are slidably provided with guide blocks 701. The charging box 7 is fixedly connected to the guide block 701. The charging box 7 is inside A clamping mechanism is provided in the part, a first clamping plate 8 is fixedly connected to the inside of the loading box 7, a guide rod 10 is slidably provided on the loading box 7, a second clamping plate 9 is fixedly connected to the guide rod 10, a first elastic member 11 is sleeved on the guide rod 10, two ends of the first elastic member 11 are respectively connected to the loading box 7 and the guide rod 10, three screws 6 are rotatably provided on the rotating frame 5, the screws 6 are threadedly connected to the guide block 701, a waterproof plate 17 is fixedly connected to the bottom of the installation box 1, the waterproof plate 17 protects the installation box 1 to prevent the installation box 1 from getting damp, a rubber pad 18 is embedded on the inner wall of the second clamping plate 9, the rubber pad 18 reduces the pressure between the second clamping plate 9 and the test tube to prevent the test tube from being damaged by excessive pressure.

[0025] When it is necessary to separate the intestinal crypt stem cells, the flap 3 is turned upward to open the installation box 1, and the torsion spring 201 is deformed accordingly. The screw rod 6 is turned to drive the guide block 701 to move, and the distance between the loading box 7 and the center point of the rotating frame 5 is adjusted, thereby adjusting the centrifugal radius of the device, so as to facilitate the adjustment of the centrifugal force to meet different research needs. After the guide block 701 is moved to a suitable position, the screw rod 6 is stopped, and the test tube containing the intestinal crypt stem cells is placed between the first clamp plate 8 and the second clamp plate 9. The test tube squeezes the second clamp plate 9 and moves outward. The first elastic member 11 is compressed accordingly, and after the test tube is placed, the test tube no longer squeezes the second clamping plate 9, and the first elastic member 11 returns to its original state so that the guide rod 10 drives the second clamping plate 9 to move inward, thereby clamping the test tube, so that the loading box 7 can hold test tubes of different types, and then the flip cover 3 is released, and the torsion spring 201 returns to its original state so that the flip cover 3 rotates downward to close the installation box 1, and then the servo motor 4 is turned on, and the output shaft of the servo motor 4 rotates to drive the rotating frame 5 to rotate, thereby causing the intestinal crypt stem cells inside the test tube to rotate, thereby achieving the purpose of separation.

[0026] Example 2: Based on Example 1, please refer to Figure 1 , Figure 4 , Figure 5 , Figure 6 and Figure 7Two mounting blocks 12 are fixedly connected to the mounting box 1, and a card slot 13 is opened on the mounting block 12. A card block 15 is slidably arranged inside the card slot 13. The contact surface between the card block 15 and the flip cover 3 is an inclined surface, so that the flip cover 3 can squeeze the card block 15 to move backward. A second elastic member 14 is connected between the card block 15 and the mounting block 12. A guide snap ring 16 is fixedly connected inside the flip cover 3, and a sealing ring 1601 is arranged on the guide snap ring 16. The sealing ring 1601 reduces the gap between the mounting box 1 and the guide snap ring 16 to seal the mounting box 1.

[0027] When the installation box 1 needs to be closed, the flip cover 3 is rotated downward to squeeze the block 15 and move it backward. The second elastic member 14 is pressed accordingly, so that the guide clamping ring 16 contacts the installation box 1 to seal the installation box 1. When the flip cover 3 is no longer squeezing the block 15, the second elastic member 14 returns to its original state to move the block 15 forward and reset it to engage with the flip cover 3, thereby limiting the flip cover 3 and preventing the flip cover 3 from rotating upward.

[0028] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An intestinal crypt stem cell separation device, comprising an installation box (1), a hinge (2), a torsion spring (201), a flap (3), a servo motor (4), a rotating frame (5), a loading box (7), a guide block (701) and a clamping mechanism, wherein two hinges (2) are installed on the installation box (1), and the two hinges (2) are provided with a flap (3) for rotation together, and two torsion springs (201) are sleeved on the hinge (2), a servo motor (4) is installed inside the installation box (1), and the output shaft of the servo motor (4) is connected to the rotating frame (5), and a plurality of guide grooves (501) are opened on the rotating frame (5), and the guide grooves (501) are slidably provided with guide blocks (701), and the guide blocks (701) are fixedly connected to the loading box (7), and the loading box (7) is provided with a clamping mechanism inside, characterized in that: It also includes a screw rod (6). A plurality of screw rods (6) are rotatably arranged on the rotating frame (5), and the screw rods (6) are threadedly connected to the guide block (701).

2. The intestinal crypt stem cell isolation device according to claim 1, characterized in that: The clamping mechanism comprises a first clamping plate (8), a second clamping plate (9), a guide rod (10) and a first elastic member (11); the first clamping plate (8) is fixedly connected inside the loading box (7); the guide rod (10) is slidably arranged on the loading box (7); the second clamping plate (9) is fixedly connected to the guide rod (10); and the first elastic member (11) is sleeved on the guide rod (10).

3. The intestinal crypt stem cell isolation device according to claim 1, characterized in that: The invention also comprises a mounting block (12), a second elastic member (14), a clamping block (15) and a guide clamping ring (16); the two mounting blocks (12) are fixedly connected to the mounting box (1); a clamping groove (13) is provided on the mounting block (12); a clamping block (15) is slidably arranged inside the clamping groove (13); a second elastic member (14) is connected between the clamping block (15) and the mounting block (12); and a guide clamping ring (16) is fixedly connected inside the flip cover (3).

4. The intestinal crypt stem cell separation device according to claim 3, characterized in that: It also includes a sealing ring (1601), and the sealing ring (1601) is arranged on the guide clamp ring (16).

5. The intestinal crypt stem cell isolation device according to claim 1, characterized in that: It also includes a water-blocking plate (17), and the bottom of the installation box (1) is fixedly connected with the water-blocking plate (17).

6. The intestinal crypt stem cell isolation device according to claim 2, characterized in that: It also includes a rubber protective pad (18), which is embedded on the inner wall of the second clamping plate (9).

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