CTC is loaded on the chip

Abstract

The utility model relates to the technical field of CTC detection, and discloses a CTC sample loading chip from above, including a cell filter and a reagent strip, and also including a sample loading gun head and a gun head seat, wherein the two sides of the top of the reagent strip are respectively provided with a first placement hole and a second placement hole, the gun head seat is arranged in the first placement hole, and the sample loading gun head is adapted to the gun head seat, the cell filter is adapted to the second placement hole, and a plurality of reagent placement cavities opened on the reagent strip are arranged between the first placement hole and the second placement hole. The utility model changes the liquid adding method in the prior art from aspiration to sample loading from above, which can not only ensure the stability and accuracy of the amount of liquid added, but also has a faster speed than sucking liquid from below, thereby reducing the detection time.

Description

Technical Field

[0001] The utility model relates to the technical field of CTC detection, in particular to a CTC top loading sample chip. Background Art

[0002] The CTC chip is a chip used for fully automatic circulating tumor cell detection, which includes a cell filter and a reagent strip. The reagent strip is loaded with various liquid reagents, and the cell filter is equipped with a screen. During the detection, the sample is pre-loaded in the cell filter, and the reagent strip and cell filter are placed in the instrument. The cell filter is controlled by the instrument to absorb and exhale the liquid reagent in the reagent strip, so that the target cells can be captured, thereby achieving the purpose of detecting the cells.

[0003] In the existing CTC detection, the transmission liquid is sucked or spit out through the cell filter. When sucking the liquid, the liquid needs to pass through the screen. Because the screen is dense and the liquid is viscous, there will be a relatively large flow resistance when sucking the liquid. The large resistance will lead to inaccurate suction, thus affecting the sensitivity of the detection. Utility Model Content

[0004] The utility model aims to solve the problems existing in the prior art and proposes a CTC sample loading chip from above.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] The CTC sample loading chip from above includes a cell filter and a reagent strip, and also includes a sample loading gun head and a gun head seat. The two sides of the top of the reagent strip are respectively provided with a first placement hole and a second placement hole. The gun head seat is arranged in the first placement hole, and the sample loading gun head is adapted to the gun head seat, and the cell filter is adapted to the second placement hole. A plurality of reagent placement cavities opened on the reagent strip are arranged between the first placement hole and the second placement hole.

[0007] Preferably, the reagent placement chamber includes a fixing liquid placement chamber, a sealing liquid placement chamber, a cleaning liquid placement chamber and a waste liquid placement chamber.

[0008] Preferably, the second placement hole is circular and the first placement hole is elliptical.

[0009] Preferably, a pipette head plug interface is provided on the top of the sample adding gun head, and the pipette head plug interface is circular.

[0010] Preferably, it also includes a control system, the control system comprising:

[0011] A syringe position control module, used to control the position of the syringe;

[0012] Syringe suction and discharge control module, used to control the syringe to suck or discharge liquid;

[0013] A syringe connected to a driving end of the syringe position control module;

[0014] The liquid-pipette head is arranged at the input and output ends of the syringe and is adapted to the liquid-pipette head plug interface.

[0015] Preferably, the syringe position control module includes an up and down moving motor, a left and right moving motor and a front and back moving motor, the driving end of the up and down moving motor is connected to the left and right moving motor, the driving end of the left and right moving motor is connected to the front and back moving motor, and the front and back moving motor is connected to the syringe.

[0016] Preferably, the syringe suction and discharge control module is a push rod motor, and a driving end of the motor is connected to the push rod of the syringe.

[0017] Compared with the prior art, the beneficial effects of the utility model are:

[0018] The utility model changes the liquid adding method in the prior art from liquid suction to adding samples from above, which can not only ensure the stability and accuracy of the amount of liquid added, but also has a faster speed than sucking liquid from below, thereby reducing the detection time. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a three-dimensional schematic diagram of the CTC sample loading chip proposed by the utility model;

[0020] Figure 2 This is an exploded schematic diagram of the CTC sample loading chip proposed by the utility model;

[0021] Figure 3 This is a top view of the CTC sample loading chip proposed by the present invention;

[0022] Figure 4 This is a schematic diagram of a control system for the CTC sample loading chip proposed by the utility model.

[0023] In the figure: 1. Cell filter; 2. Reagent strip; 3. Sample pipette tip; 4. Pipette tip seat; 5. First placement hole; 6. Second placement hole; 7. Reagent placement cavity; 8. Pipette tip plug interface; 9. Syringe; 10. Pipette tip; 11. Syringe position control module; 12. Syringe aspiration and exhalation control module. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0025] Reference Figure 1-Figure 3 The CTC sample loading chip from the top includes a cell filter 1 and a reagent strip 2, and also includes a sample loading gun head 3 and a gun head seat 4. The two sides of the top of the reagent strip 2 are respectively provided with a first placement hole 5 and a second placement hole 6. The gun head seat 4 is arranged in the first placement hole 5, and the sample loading gun head 3 is adapted to the gun head seat 4, and the cell filter 1 is adapted to the second placement hole 6. A plurality of reagent placement cavities 7 opened on the reagent strip 2 are arranged between the first placement hole 5 and the second placement hole 6.

[0026] When using this chip:

[0027] (1) First, various reagents are pre-placed in the reagent placement cavity 7 of the reagent strip 2 according to the reagent requirements, the gun tip holder 4 and the sample loading gun tip 3 are sequentially placed in the first placement hole 5 of the reagent strip 2, the cell filter 1 is placed in the second placement hole 6 of the reagent strip 2, and the sample to be tested is pre-placed in the cell filter 1, and then the reagent strip 2 is placed on the CTC detection instrument.

[0028] (2) After the instrument is turned on, the pipette tip 10 is inserted into the cell filter 1 and moved to the waste liquid chamber, the sample is slowly spit out, and then the cell filter 1 is withdrawn from the original position.

[0029] (3) Insert the pipette head 10 into the sample head, then move it to the fixative position to absorb the fixative, then move the sample head to the cell filter 1 position, add the fixative into the cell filter 1 from above, then withdraw the sample head from the gun head seat 4 position, then insert the pipette head 10 into the cell filter 1, and slowly spit out the fixative.

[0030] (4) The pipette head 10 is inserted into the pipette tip 3, and then moved to the cleaning liquid position to absorb the cleaning liquid. The pipette head is then moved to the cell filter 1 position, and the cleaning liquid is added from above into the cell filter 1. The pipette head is then withdrawn from the position of the pipette tip seat 4. Then, the pipette head 10 is inserted into the cell filter 1, and the cleaning liquid is slowly spit out.

[0031] (5) Use the same method to operate other liquid reagents.

[0032] (6) After the operation, disassemble the cell filter 1 and place it on a microscope to observe the target cells on the screen.

[0033] Compared with the existing technology, the liquid adding method is changed from aspiration to adding samples from above, which can not only ensure the stability and accuracy of the amount of liquid added, but also is faster than aspiration from below, thereby reducing the detection time.

[0034] In this embodiment, the reagent placement chamber 7 includes a fixing liquid placement chamber, a sealing liquid placement chamber, a cleaning liquid placement chamber and a waste liquid placement chamber, which is convenient for storing liquid reagents such as sealing liquid, fixing liquid, cleaning liquid, etc. respectively, and is also convenient for the placement of waste liquid.

[0035] In this embodiment, the second placement hole 6 is circular, and the first placement hole 5 is elliptical.

[0036] In this embodiment, a pipette head plug port 8 is disposed on the top of the sample pipette head 3 , and the pipette head plug port 8 is circular, so that the pipette head 10 can be easily inserted into the sample pipette head 3 .

[0037] Example 2

[0038] See also Figure 1-Figure 4 , a CTC sample loading chip from above includes a cell filter 1 and a reagent strip 2, and also includes a sample loading gun head 3 and a gun head seat 4, the two sides of the top of the reagent strip 2 are respectively provided with a first placement hole 5 and a second placement hole 6, the gun head seat 4 is arranged in the first placement hole 5, and the sample loading gun head 3 is adapted to the gun head seat 4, the cell filter 1 is adapted to the second placement hole 6, and a plurality of reagent placement cavities 7 opened on the reagent strip 2 are arranged between the first placement hole 5 and the second placement hole 6;

[0039] The reagent placement chamber 7 includes a fixing liquid placement chamber, a sealing liquid placement chamber, a cleaning liquid placement chamber, and a waste liquid placement chamber, which are convenient for storing liquid reagents such as the sealing liquid, the fixing liquid, and the cleaning liquid, respectively, and are also convenient for the placement of waste liquid;

[0040] The second placement hole 6 is circular, and the first placement hole 5 is elliptical;

[0041] The top of the sample adding gun head 3 is provided with a pipette head plug port 8, and the pipette head plug port 8 is circular;

[0042] Also included is a control system, the control system comprising:

[0043] The syringe position control module 11 is used to control the position of the syringe 9;

[0044] The syringe aspiration and discharge control module 12 is used to control the syringe 9 to aspirate or discharge liquid;

[0045] The syringe 9 is connected to the driving end of the syringe position control module 11;

[0046] The pipette head 10 is arranged at the input and output ends of the syringe 9 and is adapted to the pipette head plug interface 8 .

[0047] When using this chip:

[0048] (1) First, various reagents are pre-placed in the reagent placement cavity 7 of the reagent strip 2 according to the reagent requirements, the gun tip holder 4 and the sample loading gun tip 3 are sequentially placed in the first placement hole 5 of the reagent strip 2, the cell filter 1 is placed in the second placement hole 6 of the reagent strip 2, and the sample to be tested is pre-placed in the cell filter 1, and then the reagent strip 2 is placed on the CTC detection instrument.

[0049] (2) After the instrument is turned on, the syringe position control module 11 is used to control the pipette head 10 on the syringe 9 to be inserted into the cell filter 1 and moved to the waste liquid chamber. The syringe suction and discharge control module 12 is used to slowly discharge the sample, and then the sample is withdrawn from the cell filter 1 in situ.

[0050] (3) Insert the pipette head 10 into the sample head, then move it to the fixative position to absorb the fixative, then move the sample head to the cell filter 1 position, add the fixative into the cell filter 1 from above, then withdraw the sample head from the gun head seat 4 position, then insert the pipette head 10 into the cell filter 1, and slowly spit out the fixative.

[0051] (4) The pipette head 10 is inserted into the pipette tip 3, and then moved to the cleaning liquid position to absorb the cleaning liquid. The pipette head is then moved to the cell filter 1 position, and the cleaning liquid is added from above into the cell filter 1. The pipette head is then withdrawn from the position of the pipette tip seat 4. Then, the pipette head 10 is inserted into the cell filter 1, and the cleaning liquid is slowly spit out.

[0052] (5) Use the same method to operate other liquid reagents.

[0053] (6) After the operation, disassemble the cell filter 1 and place it on a microscope to observe the target cells on the screen.

[0054] Compared with the existing technology, the liquid adding method is changed from aspiration to adding samples from above, which can not only ensure the stability and accuracy of the amount of liquid added, but also is faster than aspiration from below, thereby reducing the detection time.

[0055] In this embodiment, the syringe position control module 11 includes an up and down moving motor, a left and right moving motor, and a front and back moving motor. The driving end of the up and down moving motor is connected to the left and right moving motor, the driving end of the left and right moving motor is connected to the front and back moving motor, and the front and back moving motor is connected to the syringe 9. The position of the syringe 9 can be changed by controlling the three motors.

[0056] In this embodiment, the syringe suction and discharge control module 12 is a push rod motor, and its driving end is connected to the push rod of the syringe 9. The push rod motor pushes the push rod of the syringe 9 to realize the movement of the piston inside the syringe 9, thereby realizing the suction and discharge of liquid.

[0057] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. CTC sample loading chip from above, including cell filter and reagent strip, characterized by: It also includes a sample loading gun head and a gun head seat, wherein the two sides of the top of the reagent strip are respectively provided with a first placement hole and a second placement hole, the gun head seat is arranged in the first placement hole, and the sample loading gun head is adapted to the gun head seat, the cell filter is adapted to the second placement hole, and a plurality of reagent placement cavities opened on the reagent strip are arranged between the first placement hole and the second placement hole; Also included is a control system, the control system comprising: A syringe position control module, used to control the position of the syringe; Syringe suction and discharge control module, used to control the syringe to suck or discharge liquid; A syringe connected to a driving end of the syringe position control module; The liquid-pipette head is arranged at the input and output ends of the syringe and is adapted to the liquid-pipette head plug interface.

2. The CTC top loading chip according to claim 1, characterized in that: The reagent placement chamber comprises a fixing liquid placement chamber, a sealing liquid placement chamber, a cleaning liquid placement chamber and a waste liquid placement chamber.

3. The CTC top loading chip according to claim 1, characterized in that: The second placement hole is circular, and the first placement hole is elliptical.

4. The CTC top loading chip according to claim 1, characterized in that: The top of the sample adding gun head is provided with a liquid pipette head plug-in interface, and the liquid pipette head plug-in interface is circular.

5. The CTC top loading chip according to claim 4, characterized in that: The syringe position control module includes an up and down moving motor, a left and right moving motor and a front and back moving motor. The driving end of the up and down moving motor is connected to the left and right moving motor, the driving end of the left and right moving motor is connected to the front and back moving motor, and the front and back moving motor is connected to the syringe.

6. The CTC top loading chip according to claim 5, characterized in that: The syringe suction and discharge control module is a push rod motor, and a driving end of the motor is connected to the push rod of the syringe.

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