Fluorescence detection module, cyclic amplification detection module and small molecular diagnostic equipment

Abstract

The utility model discloses a fluorescence detection module, a cyclic amplification detection module and a small molecular diagnostic device. The fluorescence detection module includes a composite driving mechanism, a mobile bracket, a detection module, a hot melt joint and a transceiver detection module. The composite driving mechanism can drive the mobile bracket to drive the detection module and the hot melt joint to alternately contact or stay away from the integrated detection consumables. When the hot melt joint contacts the integrated detection consumables, the hot melt joint seals the reaction chamber of the integrated detection consumables. The detection module is provided with a detection optical fiber. When the detection module conflicts with the integrated detection consumables, the reaction chamber is closely fitted between the external heat source and one end of the detection optical fiber. The other end of the detection optical fiber is connected to the transceiver detection module. The multi-channel transceiver unit in the transceiver detection module scans the second end of the detection optical fiber to obtain the real-time fluorescence result in the reaction chamber. The fluorescence detection module provided by the utility model realizes the reliable fixed constraint of the detection optical fiber, so that the detection result is accurate and reliable.

Description

Technical Field

[0001] The utility model belongs to the technical field of medical devices, and specifically relates to a fluorescence detection module, a cyclic amplification detection module and a small molecular diagnostic device. Background Art

[0002] Pathogens mainly include viruses, bacteria, mycoplasma, etc., which are mainly composed of nucleic acid molecules and protein molecules. These two types of molecules are also usually used as the main identification objects for identifying and distinguishing pathogens. The diagnostic method that uses nucleic acid molecules or nucleic acid fragments as the detection object is called molecular diagnosis. This method uses genetic material as the identification object and can accurately and quickly identify pathogens in the early stages of biological infection. Therefore, it is very important for the prevention and control of infectious diseases. In recent years, with the report of highly infectious diseases such as respiratory diseases and intestinal diseases, more and more pathogens have caused similar early symptoms. Failure to identify the correct source of infection in time will pose a severe challenge to doctors' symptomatic treatment. In some countries and regions, it is impossible to introduce corresponding detection equipment. In order to achieve complex scene detection with large changes in sample size and many detection targets, it is very necessary to develop miniaturized diagnostic equipment.

[0003] Small molecular diagnostic equipment needs to complete sample liquid processing, nucleic acid extraction and release, and amplification detection in a limited space. Under this limited condition, it is very difficult to obtain quantitative results. Chinese invention CN117025379B authorizes a RAPID constant temperature amplification nucleic acid detection device and detection method. In this scheme, the sample in the sampling tube is added and processed in the processing chamber, and then the amplified system liquid that has been processed is heated at a constant temperature to perform amplification. Finally, the amplified liquid is diverted into the chromatography type colloidal gold detection part. This design eliminates the fluorescence quantitative module and cannot achieve quantitative detection. The detection accuracy and sensitivity are relatively low. At the same time, the constant temperature amplification primer probe design is more complicated and has lower stability. Chinese invention CN117286017A discloses a multi-channel nucleic acid amplification analysis device. This scheme arranges the transmitting end and the receiving end at the bottom and side of the consumable receiving hole in a 90° cross-type. This device is more practical for pure amplification small devices, but extraction and amplification integrated devices are difficult to use. US invention US20230416811A1 discloses a device for applying heat energy to a container and detecting an emission signal from the container. In this solution, an optical fiber is inserted from the bottom of the consumable receiving part, and the inserted optical fiber is exposed to the bottom area of ​​the receiving part. A thermal cycle heating part is arranged on the side of the receiving part. Changing the position of the heating part can make the optical fiber closer to the consumables inside the consumable receiving part. This solution is also difficult to transfer to miniaturized amplification equipment. US invention US6369893B1 authorizes an optical detection solution corresponding to a flat amplification consumable, which configures the flat surface as a thermal cycle contact surface, so that the liquid in the consumable has a higher heat transfer surface, and the thickness side of the flat amplification consumable is configured with two separated transmitting ends and receiving ends, and can be configured as a multi-channel configuration to achieve multiple detection effects. This solution has specific requirements for the form of consumables. PCT application WO2023248185A1 authorizes a compact detection system, in which the transmitting light end and the receiving light end are independently configured on the same side of the detected chamber, and the transmitted light is transmitted through an optical fiber, and the two transmitting light ends can share a receiving end, and the common receiving end optical fiber is connected to a specific part to form a compact detection system. Chinese invention CN110967324A discloses an optical detection device for a multi-channel real-time fluorescence detector, in which each transmitting optical fiber and the receiving optical fiber are defined to be arranged at a predetermined angle, and a microlens array is arranged at the receiving end to improve the detection efficiency. This solution mainly optimizes the layout of the transmitting end and the receiving end to achieve the goal of the most efficient detection.

[0004] It can be seen from the existing disclosed patents that there are relatively few solutions for miniaturized diagnostic equipment to obtain amplification curves by fluorescence quantification. With the in-depth study of optical systems, the combination of the transmitting end and the receiving end and the configuration of a multi-channel multi-wavelength transceiver detection solution can maximize the execution of multiple detections in a confined space. The configuration of transmission optical fiber can make the detection more focused and the configuration of the optical system more flexible. At present, there is an urgent need to design a small integrated diagnostic equipment fluorescence detection system that matches the confined space. Utility Model Content

[0005] In order to solve the above problems existing in the prior art, the utility model is implemented through the following technical solutions:

[0006] In the first aspect of the utility model, a fluorescence detection module is provided, including a composite driving mechanism, a movable bracket, a detection module, a hot melt bonding part and a transceiver detection module, wherein the composite driving mechanism can drive the movable bracket to move in a first direction or a second direction, so that the movable bracket drives the detection module and the hot melt bonding part to alternately contact or move away from an external integrated detection consumable, and when the hot melt bonding part contacts the integrated detection consumable, the hot melt seals the reaction cavity of the integrated detection consumable, and the detection module is provided with a detection optical fiber, and when the detection module conflicts with the integrated detection consumable, the reaction cavity of the integrated detection consumable is tightly fitted between the first end of the detection optical fiber and the external heat source, and the second end of the detection optical fiber is connected to the transceiver detection module, and the transceiver detection module includes a multi-channel transceiver unit that can be driven to move, and the multi-channel transceiver unit scans the second end of the detection optical fiber to obtain real-time fluorescence results in the reaction cavity.

[0007] In one embodiment of the present invention, the detection module includes a plurality of protruding detection units, and the detection unit also includes a detection hole that passes through the detection unit body, and the detection optical fiber is configured and connected in the detection hole.

[0008] In one embodiment of the utility model, the fluorescence detection module also includes an optical fiber constraint portion, which includes a limit plate fixedly connected to the detection module, and a clamping plate cooperating with the limit plate, a flexible interlayer is arranged between the limit plate and the clamping plate, and the detection optical fiber is arranged in the flexible interlayer.

[0009] In one embodiment of the utility model, the limiting plate is a bending configuration, which includes a connecting portion fixed to the detection module and a limiting portion connected to the clamping plate, and the bending angle range between the mid-section of the limiting portion and the mid-section of the connecting portion is 55° to 85°.

[0010] In one embodiment of the present invention, the material of the flexible interlayer is sponge, and the density of the sponge is 20-28 kg / m 3 .

[0011] In one embodiment of the utility model, the transceiver detection module includes a transceiver substrate, a transceiver drive mechanism and a multi-channel transceiver unit, the transceiver substrate is provided with an optical fiber fixing hole, the second end of the detection optical fiber is connected to the optical fiber fixing hole, the transceiver drive mechanism includes a transceiver drive motor, the output of the transceiver drive motor is connected to a transceiver drive wheel, a transceiver transmission wheel is arranged at a preset interval from the transceiver drive wheel, a transceiver transmission belt is wound between the transceiver drive wheel and the transceiver transmission wheel, a transceiver transmission block is fixedly connected to one side of the transceiver transmission belt, the transceiver transmission block is also embeddedly connected to a transceiver slide rail fixedly arranged on the transceiver substrate, the transceiver transmission block is also connected to the multi-channel transceiver unit, the transceiver drive motor can drive the multi-channel transceiver unit to slide, so that the multi-channel transceiver unit can move to traverse and scan the optical fiber fixing hole.

[0012] In one embodiment of the present invention, adjacent optical fiber fixing holes are arranged at a preset interval, and the preset interval is between 1.2 and 5 times the diameter of the optical fiber fixing hole.

[0013] In the second aspect of the utility model, a cyclic amplification detection module is provided, which includes a thermal cycle module and the fluorescence detection module, wherein the fluorescence detection module is provided with a consumable receiving position, and the consumable receiving position is set as an opposing receiving groove, which can clamp the integrated detection consumable, and one side of the reaction chamber thickness direction of the integrated detection consumable is heat-conductingly connected to the thermal cycle module, and the other side of the reaction chamber thickness direction of the integrated detection consumable can alternately contact the detection module and the hot melt joint, and the thermal cycle module includes a cooling fan, a fin heat sink, a heat pipe and a heating part, the heating part is attached to the reaction chamber thickness direction of the integrated detection consumable, the heat pipe part is penetrated in the heating part, and at least part of the heat pipe is penetrated in the fin heat sink, the cooling fan, the fin heat sink and the heating part are located in the same straight line, and the air outlet direction of the cooling fan is aligned with the gap between the cooling fins of the fin heat sink.

[0014] In the third aspect of the utility model, a small molecule diagnostic device is provided, comprising a plurality of the cyclic amplification detection modules, and also comprising a second optical fiber constraint portion, wherein the second optical fiber constraint portion comprises a limit plate fixedly connected to the device and a clamping plate cooperating with the limit plate, a flexible interlayer is arranged between the limit plate and the clamping plate, and the detection optical fiber passing through one or more optical fiber fixing holes is arranged in the flexible interlayer, and the second optical fiber constraint portion can constrain the detection optical fiber of different cyclic amplification detection modules.

[0015] In one embodiment of the present invention, the small molecule diagnostic device is also configured with a control module, an air inlet, an operating cover and a closed shell, wherein the control module includes a GUI interactive system to implement operational interaction with the user, and the multi-channel transceiver unit is configured in the closed shell.

[0016] Compared with the prior art, the beneficial effects of the utility model are:

[0017] 1. The fluorescence detection module provided by the utility model receives the integrated detection consumables through the consumable receiving position, so that one side of the integrated detection consumables in the thickness direction is heat-conductingly connected to the thermal cycle module, and the other side can abut against the detection module and the hot melt joint. When the composite driving mechanism drives the movable bracket to move in the first direction or the second direction, the hot melt joint and the detection module are driven to move horizontally and alternately toward the position of the reaction chamber, so that the hot melt joint can perform hot melting and sealing on the fluid channel connected to the reaction chamber in the integrated detection consumables to form independent and sealed reaction chambers. As the composite driving mechanism drives the movable bracket to move in the opposite direction, the detection module moves toward the reaction chamber of the integrated detection consumables and applies horizontal pressure, so that the reaction chamber of the integrated detection consumables is tightly fitted with the heat source, thereby avoiding the uneven heating of the reaction chamber caused by the difference in the flatness of the reaction chamber surface and the insufficient extrusion force, and improving the sealing of the PCR reaction chamber and the close contact between the PCR reaction chamber and the heating surface, thereby improving the efficiency of the PCR reaction.

[0018] 2. The fluorescence detection module provided by the present invention changes the way the detection optical fiber is fixedly installed, and the first end of the detection optical fiber is arranged on a detection module that can be close to or away from the consumable reaction chamber. The detection optical fiber follows the detection module to perform amplified fluorescence detection, and can enable the detection module to press the reaction chamber to perform efficient detection. The second end of the detection optical fiber is connected to the transceiver detection module and can be scanned by the moving multi-channel transceiver unit to perform multi-target detection. By setting the optical fiber constraint part, the limit plate is configured as a bending structure, and bent at a specific angle, so that the detection module is fixedly connected to the limit plate, and a clamping plate that cooperates with the limit plate is configured, and a flexible interlayer is configured between the limit plate and the clamping plate, and the detection optical fiber is configured in the flexible interlayer. Even if the detection module is movable Movement will not affect the optical path and transmission efficiency of the detection optical fiber. The limit plate with a set bending angle can maximize the change of the direction of the detection optical fiber without damaging the optical fiber to ensure reliability of use. The detection optical fiber is fixed in the flexible interlayer, especially in the multi-layer sponge interlayer, so that the detection optical fiber can be reliably fixed with basically no risk of damage due to extrusion and deformation, thereby realizing reliable fixed constraint of the detection optical fiber. By plugging the second end of the detection optical fiber into the optical fiber fixing holes arranged in a line on the transceiver substrate, and driving the multi-channel transceiver unit to scan and traverse each optical fiber fixing hole through the transceiver drive mechanism, efficient and rapid detection can be achieved. The closed configuration of the multi-channel transceiver unit can shield the interference of external stray light, so that the detection result is accurate and reliable.

[0019] 3. The cyclic amplification detection module provided by the utility model, when the reaction chamber completes the preparation for the amplification reaction, the composite driving mechanism drives the movable bracket to move in the first direction, drives the hot melt joint to approach the reaction chamber, and the detection module is away from the reaction chamber, so that the hot melt joint can perform hot melting and sealing on the fluid channel connecting the integrated detection consumable and the reaction chamber, so as to form mutually independent and sealed reaction chambers, and then the composite driving mechanism drives the movable bracket to move in the second direction, drives the hot melt joint away from the reaction chamber and approaches, and multiple detection units can apply a pressing force to the reaction chamber of the consumable, and then the heating and heat dissipation control of the reaction chamber in the integrated detection consumable is realized through the thermal cycle module, so as to realize accurate PCR reaction temperature control, so that the reaction chamber in the integrated detection consumable can effectively perform PCR amplification reaction, because multiple detection units can effectively apply a pressing force to the reaction chamber of the consumable, so that the heat The heating part of the circulation module can fit the reaction chamber more closely, so that the liquid in the reaction chamber can be heated more efficiently to complete the thermal cycle. During the thermal cycle of the PCR reaction, the detection unit is driven in real time by the composite driving mechanism to contact the reaction chamber, so that the centrally arranged optical fiber can be better focused on the specific detection area in the reaction chamber, avoiding the uneven heating of the reaction chamber caused by the difference in the flatness of the reaction chamber surface and insufficient extrusion force. The multi-channel transceiver unit of the transceiver detection module traverses and scans the multiple through optical fiber fixing holes arranged in a straight line to complete the operation of transmitting and receiving light waves to obtain real-time amplification results. By improving the sealing of the PCR reaction chamber and the close contact between the PCR reaction chamber and the heating surface, the efficiency of the PCR reaction is improved, the detection result is guaranteed to be more accurate, and an integrated cyclic amplification detection module for PCR amplification reaction and detection is realized.

[0020] 4. The small molecular diagnostic device provided by the utility model includes one or more cycle amplification detection modules, which can adapt to the differentiated design of application scenarios. When there are two or more thermal cycle amplification modules, the detection optical fibers of different modules are also constrained by the second optical fiber constraint part to ensure that the system configuration is more compact and reasonable.

[0021] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the three-dimensional structure of the detection module and the hot melt bonding part in the fluorescence detection module provided in the first embodiment;

[0023] Figure 2 This is a schematic diagram of a state in which the detection module in the fluorescence detection module provided in the first embodiment is driven close to the integrated detection consumable;

[0024] Figure 3It is a schematic diagram of the connection structure between the optical fiber constraint part and the detection module in the fluorescence detection module provided in the first embodiment;

[0025] Figure 4 is an exploded schematic diagram of the optical fiber constraint portion in the fluorescence detection module provided in the first embodiment;

[0026] Figure 5 is a structural diagram of a transceiver detection module in a fluorescence detection module provided in Example 1;

[0027] Figure 6 yes Figure 5 A schematic diagram of a multi-channel transceiver unit in a transceiver detection module being driven to traverse and scan a fixed hole end of a detection optical fiber;

[0028] Figure 7 is a schematic diagram of the structure of the cyclic amplification detection module provided in Example 2;

[0029] Figure 8 yes Figure 7 A cross-sectional schematic diagram of the cyclic amplification detection module when the central detection module is pressing the integrated detection consumable reaction chamber;

[0030] Fig. 9 is a schematic structural diagram of a small molecular diagnostic device provided in Example 3;

[0031] Fig.10 It is a schematic structural diagram of another small molecular diagnostic device provided with two consumable receiving position amplification devices provided in Example 3.

[0032] Description of reference numerals:

[0033] 11-detection module; 110-detection unit; 1101-detection hole; 1102-detection optical fiber;

[0034] 12- hot melt bonding part;

[0035] 13-mobile bracket; 131-compound drive motor; 132-compound transmission block; 133-position sensing unit; 134-transmission mechanism; 134a-first connecting rod transmission mechanism; 134b-second connecting rod transmission mechanism; 135-consumable receiving position;

[0036] 14-fiber constraint part; 141-limiting plate; 142-flexible interlayer; 143-clamping plate;

[0037] 150-fiber fixing hole; 151-transceiver drive motor; 152-transceiver drive wheel; 153-transceiver drive belt; 154-transceiver drive wheel; 155-transceiver slide rail; 156-transceiver drive block; 157-transceiver drive board; 158-multi-channel transceiver unit; 159-enclosed housing;

[0038] 16- second optical fiber constraint portion;

[0039] 17-heat circulation module; 171-cooling fan; 172-fin heat sink; 173-heat pipe; 174-heating unit;

[0040] 2- small molecular diagnostic equipment; 21- operation cover; 22- control module; 23- air inlet; 24- consumable receiving position;

[0041] 3-Integrated testing consumables. DETAILED DESCRIPTION

[0042] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined purpose of the present invention, the scheme according to the present invention is described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0043] The above and other technical contents, features and effects of the utility model are clearly presented in the following detailed description of the specific implementation mode in conjunction with the accompanying drawings. Through the description of the specific implementation mode, the technical means and effects adopted by the utility model to achieve the predetermined purpose can be more deeply and specifically understood. However, the attached drawings are only for reference and explanation, and are not used to limit the technical solution of the utility model.

[0044] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants are intended to cover non-exclusive inclusion, so that an article or device including a series of elements includes not only those elements, but also other elements that are not explicitly listed. In the absence of more restrictions, the elements defined by the statement "including one..." do not exclude the existence of other identical elements in the article or device including the elements.

[0045] Embodiment 1

[0046] Please refer to Figure 1 and Figure 2 , Figure 1 is a schematic diagram of the three-dimensional structure of the detection module and the hot melt bonding part in the fluorescence detection module provided in the first embodiment, Figure 2 It is a schematic diagram of the state in which the detection module in the fluorescence detection module provided in the first embodiment is driven close to the integrated detection consumables. The fluorescence detection module includes a composite driving mechanism, a movable bracket 13, a detection module 11, a hot melt bonding part 12 and a transceiver detection module.

[0047] The composite driving mechanism can drive the movable bracket 13 to move in the first direction or the second direction, so that the movable bracket 13 drives the detection module 11 and the hot melt bonding part 12 to alternately contact or move away from the external integrated detection consumables. When the hot melt bonding part 12 contacts the integrated detection consumables, the reaction chamber of the integrated detection consumables is thermally melted and sealed. The detection module 11 is provided with a detection optical fiber 1102. When the detection module 11 conflicts with the integrated detection consumables, the reaction chamber of the integrated detection consumables is tightly fitted between the first end of the detection optical fiber 1102 and the external heat source. The second end of the detection optical fiber 1102 is connected to the transceiver detection module. The transceiver detection module includes a multi-channel transceiver unit 158 ​​that can be driven to move. The multi-channel transceiver unit 158 ​​scans the second end of the detection optical fiber to obtain real-time fluorescence results in the reaction chamber.

[0048] Further, the composite drive mechanism comprises a composite drive motor 131, a composite transmission screw and a composite transmission block 132. The composite drive motor 131 outputs a rotational motion to drive and transmit the composite transmission screw, the composite transmission screw is threadedly connected to the composite transmission block 132, and the composite transmission block 132 is connected to the mobile bracket 13, so that the composite drive motor 131 can output a rotational motion to drive the mobile bracket 13 to move up and down. Specifically, when the composite drive mechanism drives the mobile bracket 13 to move the mobile bracket 13 in the first direction, the detection module 11 is away from the integrated detection consumable, the hot melt bonding part 12 moves to the reaction chamber position of the integrated detection consumable, and the hot melt bonding part 12 heat-seals the reaction chamber of the integrated detection consumable; when the composite drive mechanism drives the mobile bracket 13 to move in the second direction, the hot melt bonding part 12 is away from the integrated detection consumable, the detection module 11 moves to the reaction chamber position of the integrated detection consumable, and the detection module 11 contacts the reaction chamber of the integrated detection consumable, so that the reaction chamber of the integrated detection consumable is closely attached to the heat source.

[0049] Further, the movable bracket 13 is respectively hingedly connected to the detection module 11 and the hot melt bonding part 12. Specifically, the movable bracket includes a transmission mechanism 134, and the transmission mechanism 134 is provided with a first connecting rod transmission mechanism 134a and a second connecting rod transmission mechanism 134b. Specifically, the first connecting rod transmission mechanism 134a includes a first fixed end rotating rod provided at the fixed connection end, a first movable bracket rotating rod provided at the movable bracket, and a first detection rotating rod provided at the detection module 11. The first fixed end rotating rod is hingedly connected to the first movable bracket rotating rod to form a first connection part, and the first movable bracket rotating rod is hingedly connected to the first detection rotating rod to form a second connection part. The angle formed by the first connection part and the second connection part in the counterclockwise direction is in the range of [85 。 -180 。), this angle range enables the detection module 11 to apply a large pressing force to the integrated detection consumables, so that when the detection module 11 moves to the reaction chamber of the integrated detection consumables, it can fully press the reaction chamber, avoid the situation where the reaction chamber is heated unevenly, and at the same time, it can be more convenient to observe the real-time fluorescence detection results in the reaction chamber of the integrated detection consumables more clearly through the detection module 11. The second connecting rod transmission mechanism 134b includes a second fixed end rotating rod arranged at the fixed connection end, a second movable bracket rotating rod arranged on the movable bracket, and a second hot melt rotating rod arranged on the hot melt bonding part 12. The second fixed end rotating rod and the second movable bracket rotating rod are hingedly connected to form a third connecting part, and the second movable bracket rotating rod and the second hot melt rotating rod are hingedly connected to form a fourth connecting part. The angle formed by the third connecting part and the fourth connecting part in the clockwise direction is in the range of [85 。 -180 。 ), this angle range enables the hot melt bonding portion 12 to apply a large pressing force to the integrated detection consumable, so that when the hot melt bonding portion 12 moves toward the integrated detection consumable, the hot melt sealing function of the reaction cavity of the integrated detection consumable is realized.

[0050] Preferably, the mobile bracket 13 also includes a consumable receiving position 135 capable of clamping the integrated detection consumable, and the consumable receiving position 135 is arranged on the same side of the detection module 11 and the hot melt bonding part 12, and a thermal circulation module with a heat transfer connection is arranged on the other side of the consumable receiving position 135 in the thickness direction. This design allows the detection module 11 and the hot melt bonding part 12 to fit more closely with the reaction chamber of the integrated detection consumable to promote the hot melt sealing and sufficient compression of the integrated detection consumable, so that the reaction chamber is heated more evenly, and the detection module can not only assume the function of compression but also enable the detection unit to be as close to the reaction chamber as possible to form a focus to make the detection result more reliable.

[0051] Furthermore, in order to ensure high-precision and high-reliability driving of the composite drive, the mobile bracket 13 is also configured with a position sensing unit 133 and a sensing sheet, and the sensing unit 133 and the sensing sheet cooperate with each other to monitor the moving height of the mobile bracket 13 .

[0052] Furthermore, the detection module 11 includes a plurality of protruding detection units 110, and the detection unit 110 as a whole can be arranged as a columnar protrusion structure. Optimally, the end of the columnar protrusion structure can be a spherical structure or a quasi-spherical structure. The detection unit 110 also includes a detection hole 1101 that passes through its body, that is, the detection hole 1101 passes through the columnar protrusion structure. The detection hole 1101 is optimally configured at the center of the detection unit 110. The first end of the detection optical fiber 1102 is configured in the detection hole 1101, and the first end of the detection optical fiber 1102 can be fixedly connected to the detection hole 1101 by heat welding, gluing, etc. The number of detection units 110 can be arranged as one or more arbitrary numbers, and each detection unit 110 corresponds to one reaction chamber. Multiple reaction chambers can divide the sample to be detected into multiple parts and enter multiple independent reaction chambers to perform amplification detection of different targets, so that the device can perform more multiple amplification detection in the same space.

[0053] In this embodiment, the detection module 11 and the hot melt bonding part 12 are integrated to form a composite functional module, the integrated detection consumables are clamped in the consumable receiving position 135, the first connecting rod transmission mechanism 134a is located above the second connecting rod transmission mechanism 134b, the detection module 11 is connected to the movable bracket 13 through the first connecting rod transmission mechanism 134a, and the hot melt bonding part 12 is connected to the movable bracket 13 through the second connecting rod transmission mechanism 134b. The movable bracket 13 moves in a first direction (in one case, the first direction here can be to make the movable bracket 13 move upward), so that the movable bracket 13 drives The hot melt bonding part 12 approaches and contacts the integrated detection consumable, and at the same time, the movable bracket 13 drives the detection module 11 away from the integrated detection consumable; the movable bracket 13 moves in the second direction, so that the movable bracket 13 drives the detection module 11 to approach or even contact the reaction chamber of the integrated detection consumable, and at the same time, the movable bracket 13 drives the hot melt bonding part 12 away from the integrated detection consumable, and the composite driving motor 131 drives the hot melt bonding part 12 and the detection module 11 alternately, so that after the hot melt bonding part 12 completes the sealing of the reaction chamber of the integrated detection consumable, the detection module 11 completes the fluorescence detection in the reaction chamber.

[0054] For further information, please refer to Figure 3 and Figure 4 , Figure 3 is a schematic diagram of the connection structure between the optical fiber constraint part and the detection module in the fluorescence detection module provided in the first embodiment, Figure 4 It is an exploded schematic diagram of the optical fiber constraint part in the fluorescence detection module provided in Example 1. The fluorescence detection module also includes an optical fiber constraint part 14, which includes a limit plate 141 fixedly connected to the detection module 11, and a clamping plate 143 cooperating with the limit plate 141, a flexible interlayer 142 is arranged between the limit plate 141 and the clamping plate 143, and the detection optical fiber 1102 is arranged in the flexible interlayer 142.

[0055] Preferably, the limiting plate 141 is a bent configuration, which includes a connection portion fixed to the detection module 11 and a limiting portion connected to the clamping plate 143. Figure 4 The horizontal connecting portion and the folded limiting portion connected to the detection module 11 are shown in the figure, and the bending angle range of the middle section of the limiting portion and the middle section of the connecting portion is 55°~85°. In other words, the angle range between the middle section of the limiting portion and the horizontal plane is 55°~85°. Such a design ensures that the detection optical fiber 1102 is configured in the optical fiber constraint portion 14 without excessive sudden bends, and the reliability of optical fiber fixation is higher, and reliable fixed constraint of the detection optical fiber can be achieved. Even if the detection module 11 moves, it will not affect the optical path direction and transmission efficiency of the detection optical fiber. The limiting plate 141 with a set bending angle can maximize the change in the direction of the detection optical fiber without damaging the optical fiber to ensure reliability of use.

[0056] Preferably, the flexible interlayer 142 is two layers and made of sponge, and the density of the sponge is 20-28 kg / m 3 The detection optical fiber is arranged in a flexible interlayer made of sponge, and is optimally arranged between two layers of flexible interlayers 142, so that the detection optical fiber can be reliably fixed without the risk of damage due to squeezing and deformation.

[0057] The fluorescence detection module provided in this embodiment, the detection module 11 includes 6 detection units 110, the center of each detection unit is plugged and fixed with the first end of the detection optical fiber 1102, the detection module 11 is fixedly connected with a fiber constraint part 14, the 6 detection optical fibers pass through the fiber constraint part 14, the detection optical fiber 1102 is at least partially fixed in the fiber constraint part 14, so that the detection optical fiber 1102 can move with the detection module 11, and the fiber constraint part 14 can reliably engage the detection optical fiber, so that the moving detection module will not cause the detection optical fiber to bend or deform, and will not cause the problem of extrusion deformation.

[0058] Please refer to Figure 5 and Figure 6 , Figure 5 is a structural diagram of a transceiver detection module in a fluorescence detection module provided in Example 1, Figure 6 yes Figure 5Schematic diagram of a multi-channel transceiver unit in a transceiver detection module being driven to traverse and scan the fixed hole end of the detection optical fiber. The transceiver detection module includes a transceiver substrate, a transceiver drive mechanism and a multi-channel transceiver unit 158. Specifically, an optical fiber fixing hole 150 is provided on the transceiver substrate, and the second end of the detection optical fiber 1102 is connected to the optical fiber fixing hole 150 on the transceiver substrate. In this embodiment, a plurality of through optical fiber fixing holes 150 arranged in a straight line are provided on the transceiver substrate, and the second end of the detection optical fiber 1102 is plugged into the optical fiber fixing hole 150. Adjacent optical fiber fixing holes 150 are arranged at a preset spacing, and the spacing distance can be between 1.2 and 5 times the diameter of the optical fiber fixing hole 150. This is because too small a hole spacing will cause the risk of detection light crosstalk and weak hole strength, and too large a hole spacing will lead to reduced detection efficiency and control accuracy. A transceiver drive mechanism is arranged on one side of the thickness direction of the transceiver substrate, and the transceiver drive mechanism includes a transceiver drive motor 151, and the output of the transceiver drive motor 151 is connected to a transceiver drive wheel 152, and a transceiver transmission wheel 154 is arranged at a preset interval from the transceiver drive wheel 152, and a transceiver transmission belt 153 is wound therebetween, and a transceiver transmission block 156 is fixedly connected to one side of the transceiver transmission belt 153, and the transceiver transmission block 156 is also connected in an engaging manner to a transceiver slide rail 155 fixedly arranged on the transceiver substrate. Optionally, the transceiver detection module also includes a transceiver drive plate 157. The transceiver drive block 156 is also connected to a transceiver drive plate 157 and a multi-channel transceiver unit 158, so that the transceiver drive motor 151 can drive the multi-channel transceiver unit 158 ​​to slide, and then the multi-channel transceiver unit 158 ​​can move through and scan the plurality of through optical fiber fixing holes 150 arranged in a straight line, complete the operation of transmitting light waves and receiving light waves to obtain the amplification result, and the multi-channel transceiver unit scans the second end of the detection optical fiber to obtain the fluorescence result in the reaction chamber. The multi-channel transceiver unit 158 ​​here can include one or more basic units of any number of transmitting units and receiving units spliced ​​and combined to achieve a combination of FAM, VIC, ROX and other detection channels, and achieve an efficient multi-channel combination. Of course, in some special cases, the transceiver drive motor can also drive the multi-channel transceiver unit 158 ​​to move through other transceiver transmission mechanisms such as a sprocket transmission mechanism or a screw slider transmission mechanism (rather than a transceiver pulley transmission mechanism). In the fluorescence detection module provided in this embodiment, the detection fiber is optically connected to the detection module 11 and the transceiver detection module, so that the functional modules of the entire fluorescence detection module can be separated and configured, and the detection functional modules can be configured without interference in a limited space.

[0059] The fluorescence detection module provided in the present embodiment receives the integrated detection consumables through the consumable receiving position, so that one side of the integrated detection consumables in the thickness direction is heat-transfer-connected to the thermal circulation module, and the other side can abut against the detection module and the hot melt bonding part. When the composite driving mechanism drives the movable bracket to move in the first direction or the second direction, the hot melt bonding part and the detection module are driven to move horizontally and alternately toward the position of the reaction chamber, so that the hot melt bonding part can perform hot melting and sealing on the fluid channel connected to the reaction chamber in the integrated detection consumables to form independent and sealed reaction chambers. As the composite driving mechanism drives the movable bracket to move in the opposite direction, the detection module moves toward the reaction chamber of the integrated detection consumables and applies horizontal pressure, so that the reaction chamber of the integrated detection consumables is tightly fitted with the heat source, thereby avoiding the uneven heating of the reaction chamber caused by the difference in the flatness of the reaction chamber surface and the insufficient extrusion force, and improving the sealing of the PCR reaction chamber and the close contact between the PCR reaction chamber and the heating surface, thereby improving the efficiency of the PCR reaction. In addition, the fluorescence detection module provided in the present embodiment changes the way the detection optical fiber is fixedly installed, and the first end of the detection optical fiber is configured on a detection module that can be close to or away from the consumable reaction chamber. The detection optical fiber follows the detection module to perform amplification detection, and can enable the detection module to press the reaction chamber to perform efficient detection. The second end of the detection optical fiber is connected to the transceiver detection module and can be scanned by the moving multi-channel transceiver unit to perform multi-target detection. By setting the optical fiber constraint part, the limit plate is configured as a bending structure, and bent at a specific angle, so that the detection module is fixedly connected to the limit plate, and a clamping plate that cooperates with the limit plate is configured, and a flexible interlayer is configured between the limit plate and the clamping plate, and the detection optical fiber is configured in the flexible interlayer. Even if the detection module moves, It will not affect the optical path and transmission efficiency of the detection optical fiber. The limit plate with a set bending angle can maximize the change of the direction of the detection optical fiber, and will not damage the optical fiber to ensure the reliability of use. By fixing the detection optical fiber in the flexible interlayer, especially between the multiple layers of sponge interlayers, the detection optical fiber can be reliably fixed with basically no risk of damage due to extrusion and deformation, thereby realizing reliable fixed constraint of the detection optical fiber. By plugging the second end of the detection optical fiber into the optical fiber fixing holes arranged in a line on the transceiver substrate, and driving the multi-channel transceiver unit to scan and traverse each optical fiber fixing hole through the transceiver drive mechanism, efficient and rapid detection can be achieved. The closed configuration of the multi-channel transceiver unit can shield the interference of external stray light, so that the detection result is accurate and reliable.

[0060] Embodiment 2

[0061] Please refer to Figure 7 and Figure 8 , Figure 7 is a schematic diagram of the structure of the cyclic amplification detection module provided in Example 2, Figure 8 yes Figure 7The cross-sectional schematic diagram of the cyclic amplification detection module when the detection module is pressed against the integrated detection consumable reaction chamber, this embodiment provides a cyclic amplification detection module, which includes a thermal cycle module 17 and the fluorescence detection module in the first embodiment.

[0062] Specifically, the fluorescence detection module is also provided with a consumable receiving position 135, wherein the consumable receiving position 135 is configured to include two vertically arranged opposing receiving grooves, which can snap into the integrated detection consumable 3, and one side of the reaction chamber thickness direction of the integrated detection consumable 3 is heat-transferably connected to the thermal circulation module 17, and the other side of the reaction chamber thickness direction of the integrated detection consumable 3 can alternately contact the detection module 11 and the hot melt bonding portion 12. The alternate contact between the reaction chamber and the detection module 11 and the hot melt bonding portion 12 has been described in detail in Example 1 and will not be repeated here. The thermal circulation module 17 includes a cooling fan 171, a fin heat sink 172, a heat pipe 173 for heat transfer and a heating part 174 for providing a heat source. The heating part 174 is attached to the thickness direction of the reaction chamber of the integrated detection consumable 3. The heat pipe 173 is partially penetrated in the heating part, and the heat pipe 173 is at least partially penetrated in the fin heat sink 172. Specifically, the heat pipe 173 includes a group of pipe groups or multiple groups of pipe groups arranged at intervals. Each group of pipe groups includes a heat conduction part, a first heat dissipation part and a second heat dissipation part. The heat conduction part is embedded in the heating part 174. The first heat dissipation part penetrates the fin heat sink 172 along the length direction of the fin heat sink 172 and is connected to the first end of the heat conduction part. The second heat dissipation part penetrates the fin heat sink 172 along the length direction of the fin heat sink 172 and is connected to the second end of the heat conduction part. The penetration direction of the first heat dissipation part in the fin heat sink 172 is different from the penetration direction of the second heat dissipation part in the fin heat sink 172. The cooling fan 171, the fin heat sink 172 and the heating part 174 are located in the same straight line, and the air outlet direction of the cooling fan 171 is aligned with the gap between the cooling fins of the fin heat sink 172, so that the heat of the heating part can be transferred to the fin heat sink 172 to achieve efficient heat dissipation.

[0063] The cyclic amplification detection module provided in this embodiment receives the integrated detection consumable 3 through the consumable receiving position 135, and is connected to the thermal cycle module 17 along one side of the thickness direction of the integrated detection consumable by heat transfer, and the other side can abut against the detection module 11 and the hot melt joint 12. When the reaction chamber completes the preparation for the amplification reaction, the composite driving mechanism drives the mobile bracket to move in the first direction, drives the hot melt joint to approach the reaction chamber, and the detection module moves away from the reaction chamber, so that the hot melt joint can perform hot fusion sealing on the fluid channel connected to the reaction chamber in the integrated detection consumable to form a mutually independent and sealed reaction chamber. Then, the composite driving mechanism drives the mobile bracket to move in the second direction, drives the hot melt joint away from the reaction chamber, and multiple detection units 110 can apply a pressing force to the reaction chamber of the consumable. Then, the heating and heat dissipation control of the reaction chamber in the integrated detection consumable is realized through the thermal cycle module, and accurate PCR reaction temperature control is realized, so that the reaction chamber in the integrated detection consumable can effectively perform PCR amplification reaction. Since the multiple detection units 110 can effectively apply a pressing force to the reaction chamber of the consumable, the heating part of the thermal cycle module can The multi-channel transceiver unit 158 ​​of the transceiver detection module traverses and scans the plurality of through optical fiber fixing holes 150 arranged in a straight line to complete the operation of transmitting and receiving light waves to obtain real-time amplification results. By improving the sealing of the PCR reaction chamber and the close contact between the PCR reaction chamber and the heating surface, the efficiency of the PCR reaction is improved, the detection result is guaranteed to be more accurate, and the integrated cyclic amplification detection module of PCR amplification reaction and detection is realized.

[0064] Embodiment 3

[0065] Please refer to Fig. 9 and Fig.10 , Fig. 9 is a schematic diagram of the structure of a small molecular diagnostic device provided in Example 3, Fig.10It is a structural schematic diagram of another small molecule diagnostic device provided with two consumable receiving amplification devices provided in Example 3. The small molecule diagnostic device includes one or more cyclic amplification detection modules in Example 2. In order to ensure that the small molecule diagnostic device provided with multiple cyclic amplification detection modules is more compact and reasonable, the small molecule diagnostic device is also provided with a second optical fiber constraint portion 16. The second optical fiber constraint portion 16 can constrain the detection optical fibers of different cyclic amplification detection modules. The components of the second optical fiber constraint portion 16 are the same as the optical fiber constraint portion 14 on the detection module 11. The second optical fiber constraint portion 16 includes a limit plate fixedly connected to the device and a clamping plate matched with the limit plate. A flexible interlayer is arranged between the limit plate and the clamping plate. The detection optical fiber 1102 passing through one or more optical fiber fixing holes 150 is arranged in the flexible interlayer. The second optical fiber constraint portion can constrain the detection optical fibers of different cyclic amplification detection modules. The front of the small molecule diagnostic device 2 is also configured with an operating cover 21, a control module 22, an air inlet 23 and a closed shell 159. The control module 22 includes a GUI interactive system that can realize operational interaction with the user. The bottom of the front is also configured with an air inlet 23 to transport external air into the device to achieve the effects of heat dissipation and air exchange. The small molecule diagnostic device 2 includes two consumable receiving positions 135, and the snap-connected operating cover 21 can snap and press the integrated detection consumable 3 into one of the consumable receiving positions 135 to carry out detection operations. In order to minimize the interference of external stray light on the multi-channel transceiver unit 158, the multi-channel transceiver unit 158 ​​is configured in the closed shell 159, and the inner wall surface of the closed shell 159 can be covered with shading material to ensure the accuracy of the detection.

[0066] In this embodiment, the small molecular diagnostic device includes two cyclic amplification detection modules. Of course, in some cases, in order to make the small molecular diagnostic device smaller, only one cyclic amplification detection module can be configured. In another case, a larger number of cyclic amplification detection modules can be configured in the device, which is not limited here. The small molecular diagnostic device provided in this embodiment includes one or more cyclic amplification detection modules, which can adapt to the differentiated design of application scenarios. When there are two or more thermal cycle amplification modules, the detection optical fibers of different modules are also constrained by the second optical fiber constraint part to ensure that the system configuration is more compact and reasonable.

[0067] The above contents are further detailed descriptions of the present invention in combination with specific preferred implementations, and the specific implementation of the present invention cannot be considered to be limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. A fluorescence detection module, characterized in that: It comprises a composite driving mechanism, a movable bracket (13), a detection module (11), a hot melt bonding part (12) and a transceiver detection module, wherein: The composite driving mechanism can drive the movable bracket (13) to move in a first direction or a second direction, so that the movable bracket (13) drives the detection module (11) and the hot melt bonding part (12) to alternately contact or move away from the external integrated detection consumables. When the hot melt bonding part (12) contacts the integrated detection consumables, the integrated detection consumables reaction chamber is sealed by hot fusion. The detection module (11) is provided with a detection optical fiber (1102). When the detection module (11) and the integrated detection consumables are in conflict, the reaction chamber of the integrated detection consumables is tightly fitted between the first end of the detection optical fiber (1102) and the external heat source. The second end of the detection optical fiber (1102) is connected to the transceiver detection module. The transceiver detection module includes a multi-channel transceiver unit (158) that can be driven to move. The multi-channel transceiver unit (158) scans the second end of the detection optical fiber to obtain real-time fluorescence results in the reaction chamber.

2. The fluorescence detection module according to claim 1, characterized in that: The detection module (11) comprises a plurality of protruding detection units (110), the detection units (110) further comprising a detection hole (1101) penetrating the body of the detection unit (110), the detection optical fiber (1102) being arranged in the detection hole (1101) for connection.

3. The fluorescence detection module according to claim 1, characterized in that: The optical fiber restraint part (14) further comprises an optical fiber restraint part (14), wherein the optical fiber restraint part (14) comprises a limit plate (141) fixedly connected to the detection module (11), and a clamping plate (143) matched with the limit plate (141), a flexible interlayer (142) is arranged between the limit plate (141) and the clamping plate (143), and the detection optical fiber (1102) is arranged in the flexible interlayer (142).

4. The fluorescence detection module according to claim 3, characterized in that: The limiting plate (141) is a bending configuration, which includes a connection portion fixed to the detection module (11) and a limiting portion connected to the clamping plate (143), and the bending angle range between the middle section of the limiting portion and the middle section of the connecting portion is 55° to 85°.

5. The fluorescence detection module according to claim 3, characterized in that: The material of the flexible interlayer (142) is sponge, and the density of the sponge is 20-28 kg / m 3 .

6. The fluorescence detection module according to claim 1, characterized in that: The transceiver detection module comprises a transceiver substrate, a transceiver drive mechanism and a multi-channel transceiver unit (158); the transceiver substrate is provided with an optical fiber fixing hole (150); the second end of the detection optical fiber (1102) is connected to the optical fiber fixing hole (150); the transceiver drive mechanism comprises a transceiver drive motor (151); the output of the transceiver drive motor (151) is connected to a transceiver drive wheel (152); a transceiver transmission wheel (154) is arranged at a preset interval from the transceiver drive wheel (152); the transceiver drive wheel (152) and the transceiver transmission wheel (154) are connected to the transceiver drive wheel (152); A transceiver transmission belt (153) is wound around the optical fiber (154), a transceiver transmission block (156) is fixedly connected to one side of the transceiver transmission belt (153), the transceiver transmission block (156) is also connected in an engaging manner to a transceiver slide rail (155) fixedly arranged on the transceiver substrate, the transceiver transmission block (156) is also connected to a multi-channel transceiver unit (158), and the transceiver drive motor (151) can drive the multi-channel transceiver unit (158) to slide, so that the multi-channel transceiver unit (158) can move to traverse and scan the optical fiber fixing hole (150).

7. The fluorescence detection module according to claim 6, characterized in that: Adjacent optical fiber fixing holes (150) are arranged at a preset interval, and the preset interval is between 1.2 and 5 times the diameter of the optical fiber fixing hole (150).

8. A cyclic amplification detection module, characterized in that: It comprises a thermal cycle module (17) and a fluorescence detection module according to claim 1, wherein: The fluorescence detection module is provided with a consumable receiving position (135), and the consumable receiving position (135) is set as an opposite receiving groove, and the opposite receiving groove can be clamped with the integrated detection consumable (3), and one side of the reaction chamber thickness direction of the integrated detection consumable (3) is heat-conductingly connected to the thermal circulation module (17), and the other side of the reaction chamber thickness direction of the integrated detection consumable (3) can alternately contact the detection module (11) and the hot melt bonding part (12), and the thermal circulation module (17) includes a cooling fan (171), a fin heat sink (17 2), a heat pipe (173) and a heating part (174), the heating part (174) is attached to the thickness direction of the reaction chamber of the integrated detection consumable (3), the heat pipe (173) is partially penetrated in the heating part (174), and the heat pipe (173) is at least partially penetrated in the fin heat sink (172), the heat dissipation fan (171) is located in the same straight line as the fin heat sink (172) and the heating part (174), and the air outlet direction of the heat dissipation fan (171) is aligned with the gap between the heat dissipation fins of the fin heat sink (172).

9. A small molecular diagnostic device, characterized in that: The method comprises a plurality of cyclic amplification detection modules according to claim 8, and further comprises a second optical fiber constraint (16), wherein: The second optical fiber restraint part (16) includes a limit plate fixedly connected to the device and a clamping plate cooperating with the limit plate, a flexible interlayer is arranged between the limit plate and the clamping plate, and the detection optical fiber (1102) passing through one or more optical fiber fixing holes (150) is arranged in the flexible interlayer, and the second optical fiber restraint part can restrain the detection optical fiber of different cycle amplification detection modules.

10. The small molecular diagnostic device according to claim 9, characterized in that: It is also provided with a control module (22), an air inlet (23), an operating cover (21) and a closed housing (159), wherein: The control module (22) includes a GUI interactive system to implement operational interaction with the user, and the multi-channel transceiver unit (158) is configured in a closed housing (159).

Images