Liquid cover film cover sheet

Abstract

The utility model discloses a liquid cover film cover sheet, which can take into account the operability of adding sample reagent liquid, and at the same time improve the sealing effect of the microenvironment formed under the liquid cover film cover sheet during the experiment on the sample adding side. It includes a homogeneous cover part and a clamping part, the bottom surface of the cover part is respectively provided with a first current limiting strip extending along the longitudinal direction of the cover part, and a reaction chamber is between the two current limiting strips, one end of the cover part is connected to the clamping part, the bottom surface of the cover part is provided with a second current limiting strip at one end away from the clamping part, and the top surface of the cover part is provided with a horizontally extending chute at one end away from the clamping part, the bottom of the chute is provided with a first liquid adding slit of the connected reaction chamber, a baffle is slidably connected in the chute, and the baffle can slide in the chute along the longitudinal direction of the cover part, an elastic strip is provided between a side wall of the chute close to the clamping part and the baffle, and the baffle is provided with a second liquid adding slit and a liquid adding port.

Description

Technical Field

[0001] The utility model relates to the technical field of immunohistochemical experiments, in particular to a cover sheet structure in a liquid cover film method immunohistochemical experiment. Background Art

[0002] Immunohistochemistry is based on the principle of antigen-antibody reaction, that is, the specific binding of antigen and antibody. It uses chemical reactions to make the color developing agents such as fluorescein, enzymes, metal ions, and isotopes labeled with antibodies develop color to determine the antigenic peptides and proteins in tissue cells, and conduct localization, qualitative and quantitative research on them.

[0003] In recent years, researchers in this field have developed fully automatic individual temperature-controlled liquid-covered immunohistochemistry detection instruments, realizing fully automatic individual temperature-controlled liquid-covered immunohistochemistry equipment with independent temperature control and heating for each slide. It can replace manual automation to complete operations such as staining, sealing and washing in immunohistochemistry, improve experimental operation efficiency and accuracy, and realize batch immunohistochemistry experiments.

[0004] The bottom surface of the liquid cover film cover has a concave reaction chamber, the depth of the reaction chamber is very small, usually less than 1 mm, and the liquid cover film cover has a mechanical structure to push the cover to be set on the glass slide, and the reaction chamber and the upper surface of the glass slide form a microenvironment for reaction. The plane where the cover and the glass slide fit together is at a certain inclination angle. The reagent liquid is added at one end, and the gravity of the fluid and the siphon phenomenon of the narrow space for the liquid are used to make the reagent liquid enter the reaction chamber, and the gas therein is driven out, and the reaction chamber is filled with the reagent liquid. However, the cover design scheme of the prior art needs to have an opening gap for adding liquid at one end of the liquid adding end, which affects the closedness of the microenvironment, thereby affecting the final use effect, and has high process requirements for the liquid cover film cover. Utility Model Content

[0005] The utility model aims to overcome the above-mentioned shortcomings of the liquid cover membrane cover sheet in the prior art and provides a liquid cover membrane cover sheet, which can take into account the operability of adding sample reagent liquid and at the same time improve the sealing effect of the microenvironment formed under the liquid cover membrane cover sheet on the sample adding side during the experiment.

[0006] The lid of the present invention relates to a liquid cover membrane cover sheet, comprising a homogeneous covering portion and a clamping portion, wherein two sides of the bottom surface of the covering portion are respectively provided with a first flow limiting strip extending along the longitudinal direction of the covering portion, and a reaction chamber is formed between the two flow limiting strips, one end of the covering portion is connected to the clamping portion, and a second flow limiting strip is provided on the bottom surface of the covering portion away from the clamping portion, the second flow limiting strip is vertically arranged at the end of the first flow limiting strip and connected to the first flow limiting strip, and a transversely extending slide groove is provided at the end of the top surface of the covering portion away from the clamping portion, and a first liquid adding slit of the communicating reaction chamber is provided at the bottom of the slide groove, a baffle strip is slidably connected in the slide groove, and the baffle strip can slide in the slide groove along the longitudinal direction of the covering portion, an elastic strip is provided between a side wall of the slide groove close to the clamping portion and the baffle strip, the baffle strip is provided with a second liquid adding slit and a liquid adding port, the liquid adding port is located in the middle of the top surface of the baffle strip, the liquid adding port is connected with the second liquid adding slit, and connecting columns are provided at both ends of the baffle strip.

[0007] Preferably, the transverse width of the first liquid adding gap is consistent with the transverse width of the second liquid adding gap.

[0008] Preferably, the elastic strip is a hollow silicone strip, which is used to apply elastic reset force to the baffle when the elastic strip is compressed by the baffle, and when the elastic strip is not compressed, the second liquid adding slot in the baffle is opposite to the first liquid adding slot at the bottom of the slide groove.

[0009] Preferably, the bottom end of the first liquid adding slot extends to an end of the reaction chamber close to the second current limiting strip.

[0010] Preferably, a groove is provided on one side of the baffle bar close to the elastic bar, a baffle is inserted between the groove and the elastic bar, and a gap between the baffle and the groove constitutes a second liquid adding gap.

[0011] Preferably, an "inverted L"-shaped limiting member is provided at both transverse ends of the slide groove, and the blocking bar passes through the space between the lower part of the "inverted L"-shaped structure of the limiting member and the slide groove.

[0012] Preferably, a bayonet is provided at one end of the clamping portion away from the covering portion.

[0013] Preferably, a rectangular weight-reducing groove is provided in the middle of the top surface of the covering portion, and the side walls surrounding the weight-reducing groove form a reinforcing rib structure.

[0014] This solution optimizes the liquid adding path into two sections, the first liquid adding slit and the second liquid adding slit. The second liquid adding slit is set on the baffle and can move with the baffle. When the first liquid adding slit and the second liquid adding slit are opposite, the reagent is added at the liquid adding port, and flows down through the second liquid adding slit and the first liquid adding slit to the end of the reaction chamber close to the second flow limiting strip in turn, so that the reaction chamber can be smoothly filled and the air can be discharged. The liquid cover film cover of this solution, which is the same as the prior art, is connected to the control mechanism of the immunohistochemistry equipment by the clamping part to perform the covering operation. At the same time, the connecting columns at both ends of the baffle are also overlapped on the slide rack to play the role of supporting and guiding the limit. Since the baffle in this solution can slide, the control mechanism can slightly push the liquid cover film cover again, and the connecting column is limited, so that the baffle slides relative to the slide groove, causing the first liquid adding slit and the second liquid adding slit to be misaligned, closing the liquid adding path, thereby further ensuring the closedness of the microenvironment formed under the liquid cover film cover in the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a structural schematic diagram of the utility model.

[0016] Figure 2 It is a sectional view of the utility model from a side viewing angle.

[0017] Figure 3 yes Figure 2 A partial enlarged view of .

[0018] Note in the figure:

[0019] 101, cover part; 102, clamping part; 103, bayonet; 104, weight reduction groove

[0020] 201, first current limiting strip; 202, second current limiting strip; 203, reaction chamber

[0021] 301, slide groove; 302, first liquid adding gap; 303, limiter

[0022] 401, baffle; 402, connecting column; 403, elastic strip; 404, second liquid adding gap; 405, liquid adding port; 406, baffle. DETAILED DESCRIPTION

[0023] The utility model is further described below in conjunction with the accompanying drawings and specific implementation methods.

[0024] like Figure 1 , Figure 2 , Figure 3As shown, a liquid cover film cover sheet of the present application comprises a homogeneous cover part 101 and a clamping part 102, and first current limiting strips extending longitudinally along the cover part are respectively provided on both sides of the bottom surface of the cover part, and a reaction chamber 203 is provided between the two current limiting strips, one end of the cover part is connected to the clamping part, and a second current limiting strip 202 is provided at one end of the bottom surface of the cover part away from the clamping part, and the second current limiting strip is vertically arranged at the end of the first current limiting strip and connected to the first current limiting strip 201. Preferably, the vertical height difference between the lower end surfaces of the first current limiting strip and the second current limiting strip and the bottom surface of the cover part is 0.05-0.7 mm.

[0025] A transversely extending slide groove 301 is arranged at one end of the top surface of the covering part away from the clamping part, and a first liquid adding slot 302 connected to the reaction chamber is arranged at the bottom of the slide groove, and the bottom end of the first liquid adding slot extends to one end of the reaction chamber near the second current limiting strip.

[0026] A stop bar 401 is slidably connected in the slide groove, and the stop bar can slide in the slide groove along the longitudinal direction of the covering part. Specifically, the two ends of the slide groove are connected, and an "inverted L"-shaped limiter 303 is arranged at both ends of the slide groove in the horizontal direction, and the stop bar passes through the space between the lower part of the "inverted L"-shaped structure of the limiter and the slide groove.

[0027] An elastic strip 403 is provided between a side wall of the slide close to the clamping part and the baffle, and the baffle is provided with a second liquid-adding slit 404 and a liquid-adding port 405. A groove is provided on one side of the baffle close to the elastic strip, and a baffle 406 is inserted between the groove and the elastic strip, and the gap between the baffle and the groove constitutes the second liquid-adding slit. The liquid-adding port is located in the middle of the top surface of the baffle, and the liquid-adding port is connected to the second liquid-adding slit, and connecting columns 402 are provided at both ends of the baffle.

[0028] The transverse width of the first liquid adding gap is consistent with the transverse width of the second liquid adding gap.

[0029] The elastic strip is a hollow silicone strip, which is used to apply elastic reset force to the baffle when it is compressed by the baffle. When the elastic strip is not compressed, the second liquid adding slot in the baffle is opposite to the first liquid adding slot at the bottom of the chute.

[0030] The end of the clamping part away from the covering part is provided with a clamping hole 103. A rectangular weight-reducing groove 104 is provided in the middle of the top surface of the covering part, and the side wall surrounding the weight-reducing groove forms a reinforcing rib structure.

[0031] The technical solution of the present application adopts a different reagent adding method from the prior art. First, the second current limiting strip replaces the original opening gap for adding liquid, so that the second current limiting strip and the original first current limiting strip form a closed structure surrounding the reaction chamber and one end, thereby ensuring the microenvironment formed under the liquid cover film cover sheet in the experiment, and the closed effect on the sample adding side.

[0032] This solution optimizes the liquid adding path into two sections, the first liquid adding slot and the second liquid adding slot. The second liquid adding slot is set on the baffle bar and can move with the baffle bar. When the first liquid adding slot and the second liquid adding slot are opposite to each other, the reagent is added at the liquid adding port, and flows down through the second liquid adding slot and the first liquid adding slot in turn to the end of the reaction chamber near the second flow limiting strip, so that the reaction chamber can be smoothly filled and the air can be discharged.

[0033] The liquid cover film cover sheet of this scheme, which is the same as the prior art, is connected to the control mechanism of the immunohistochemical equipment by the clamping part to perform the covering operation, and the connecting columns at both ends of the baffle are also overlapped on the glass slide rack to play the role of support and guide limit. Since the baffle in this scheme can slide, the control mechanism can slightly push the liquid cover film cover sheet again, and the connecting column is limited, so that the baffle slides relative to the slide groove, so that the first liquid addition gap and the second liquid addition gap are misaligned, and the liquid addition path is closed, thereby further ensuring the closedness of the microenvironment formed under the liquid cover film cover sheet in the experiment.

Claims

1. A liquid cover membrane cover sheet, comprising a homogeneous cover portion and a clamping portion, wherein both sides of the bottom surface of the cover portion are respectively provided with first current limiting strips extending longitudinally along the cover portion, a reaction chamber is provided between the two current limiting strips, one end of the cover portion is connected to the clamping portion, and is characterized in that: A second current limiting strip is provided at one end of the bottom surface of the covering portion away from the clamping portion, the second current limiting strip is vertically arranged at the end of the first current limiting strip and connected to the first current limiting strip, a transversely extending slide groove is provided at one end of the top surface of the covering portion away from the clamping portion, a first liquid adding slit of a connected reaction chamber is provided at the bottom of the slide groove, a baffle bar is slidably connected in the slide groove, the baffle bar can slide in the slide groove along the longitudinal direction of the covering portion, an elastic strip is provided between a side wall of the slide groove close to the clamping portion and the baffle bar, the baffle bar is provided with a second liquid adding slit and a liquid adding port, the liquid adding port is located in the middle of the top surface of the baffle bar, the liquid adding port is connected with the second liquid adding slit, and connecting columns are provided at both ends of the baffle bar.

2. A liquid cover film cover sheet according to claim 1, characterized in that: The transverse width of the first liquid adding gap is consistent with the transverse width of the second liquid adding gap.

3. A liquid cover film cover sheet according to claim 2, characterized in that: The elastic strip is a hollow silicone strip, which is used to apply elastic reset force to the baffle when it is compressed by the baffle. When the elastic strip is not compressed, the second liquid adding slot in the baffle is opposite to the first liquid adding slot at the bottom of the chute.

4. A liquid cover film cover sheet according to claim 1, characterized in that: The bottom end of the first liquid adding slot extends to an end in the reaction chamber close to the second current limiting strip.

5. A liquid cover film cover sheet according to claim 1, 2 or 3, characterized in that: A groove is provided on one side of the baffle bar close to the elastic bar, a baffle is inserted between the groove and the elastic bar, and a gap between the baffle and the groove constitutes a second liquid adding gap.

6. A liquid cover film cover sheet according to claim 1, characterized in that: An "inverted L"-shaped limiting piece is arranged at both transverse ends of the slide slot, and the blocking bar passes through the space between the lower part of the "inverted L"-shaped structure of the limiting piece and the slide slot.

7. A liquid cover film cover sheet according to claim 1, characterized in that: A bayonet is provided at one end of the clamping portion away from the covering portion.

8. The liquid cover film cover sheet according to claim 1, characterized in that: A rectangular weight-reducing groove is provided in the middle of the top surface of the covering portion, and a reinforcing rib structure is formed around the side walls of the weight-reducing groove.

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