Cell fixing, density estimating and shaping integrated device
By designing a cell processing device that integrates centrifugal diversion and density estimation functions, the problem of cumbersome and inefficient processing of shed cell samples in the prior art is solved, and efficient and simplified cell fixation, density estimation and shaping processes are achieved.
Patent Information
- Application Number
- CN202421917007.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-08
- Publication Date
- 2025-04-15
- Estimated Expiration
- 2034-08-08
AI Technical Summary
In the prior art, processing of shed cell samples requires multiple different devices, which are complicated to operate, low efficiency, and the prepared cell clumps are different in diameter, which is prone to breaking and losing cells.
Design an integrated cell fixation, density estimation and shaping device, including a centrifugal diversion assembly and density estimation assembly, centrifugal separation processing and supernatant diversion pouring, roughly estimate cell density through density estimation assembly, simplify operational steps and improve processing efficiency.
It realizes efficient processing of shed cell samples, simplifies operation steps, improves processing efficiency, and produces cell clumps of the same diameter, suitable for subsequent preparation of standard specification stained sheets.
Smart Images
Figure CN222758217U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to a cell processing device, in particular to a cell fixing, density estimation and shaping integrated device. Background Art
[0002] Cells shed by the natural renewal of the epithelium of human organs are called exfoliated cells. Common clinical samples containing exfoliated cells mainly include: natural exudates such as urine, sputum, milk, and semen, body cavity extracts such as pleural effusion, ascites, pericardial effusion, cerebrospinal fluid, ovarian cyst fluid, and body cavity scraping cells such as cervix and oral cavity. Exfoliated cell samples can provide an important reference for the diagnosis of various diseases, and have the advantages of simple sampling, repeatability, little damage to patients, and high compliance. With the development of technology, the role of exfoliated cell samples in the non-invasive screening, diagnosis and follow-up of various diseases, especially malignant tumors, is becoming increasingly prominent.
[0003] In inspection and diagnosis, the processing of exfoliated cell samples usually requires fixation, density estimation, centrifugation and shaping to form cell clumps. The cell clumps are dehydrated and paraffin-embedded to make cell wax blocks. Traditionally, multiple devices are used for cell fixation, density estimation, centrifugation and shaping. In the process of preparing cell clumps, different devices often need to be replaced multiple times, which is cumbersome and greatly reduces the preparation efficiency. In addition, the diameters of the prepared cell clumps vary and are easy to break and lose cells. Utility Model Content
[0004] In view of this, the utility model provides a cell fixation, density estimation and shaping integrated device. The purpose is to provide a cell fixation, density estimation and shaping integrated device with simple operation, higher efficiency and better application effect.
[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:
[0006] A cell fixation, density estimation and shaping integrated device, comprising a centrifugal flow guide component and a density estimation component arranged on the outer side of the lower part of the centrifugal flow guide component; the centrifugal tube body component comprises a centrifugal tube, the top periphery of the centrifugal tube is correspondingly threadedly connected with an upper cover, the bottom periphery of the centrifugal tube is correspondingly threadedly connected with a lower cover, the centrifugal tube is connected with a plurality of flow guide tubes, the top periphery of the flow guide tube is correspondingly screwed with a screw cap, one side of the screw cap is correspondingly integrally formed with two elastic blocks, a limiting groove is provided on the upper cover corresponding to the screw cap, and two limiting blocks are arranged in the limiting groove corresponding to the two elastic blocks; the density estimation component comprises a placement groove arranged on the rear side of the centrifugal tube corresponding to the bottom periphery of the centrifugal tube, a density indication label is detachably placed in the placement groove, and two density indication lines are symmetrically arranged in the middle part of the density indication label.
[0007] Preferably, the centrifuge tube is provided with a stirring assembly, and the stirring assembly includes a stirring rod, the top of the stirring rod is clamped in the groove at the center of the bottom of the upper cover, and a plurality of stirring blades are evenly arranged on the periphery of the stirring rod, and a fixing ring is fixedly arranged on the bottom of the stirring rod by a plurality of connecting rods, and the bottom periphery of the fixing ring is threadedly connected to the fixing groove at the bottom of the centrifuge tube accordingly.
[0008] Preferably, an arc-shaped groove is provided at the left end of the outer groove body of the placement groove.
[0009] Preferably, a limiting ring is integrally formed on the periphery of the top of the fixing ring corresponding to the inner wall of the centrifuge tube.
[0010] Preferably, a plurality of the connecting rods are arranged in a ring array between the stirring rod and the fixing ring.
[0011] Preferably, the plurality of flow guide tubes are evenly distributed on the centrifuge tube, and the plurality of flow guide tubes are located in different horizontal planes, and the flow guide tubes are made of high-performance polyolefin thermoplastic elastomer (TPE) material.
[0012] Preferably, the centrifuge tube, the upper cover and the lower cover are all made of ABS material.
[0013] The beneficial effects of the utility model are:
[0014] 1. The utility model includes a centrifugal flow guide component and a density estimation component arranged on the outer side of the lower part of the centrifugal flow guide component. This arrangement uses the centrifugal flow guide component to centrifuge the exfoliated cell sample, drain the supernatant, and assist in the production of cell agglomerates. The density of the exfoliated cell sample is roughly estimated by the density estimation component. In general, compared with the previous integrated device for cell fixation, density estimation and shaping, this device can sequentially centrifuge the exfoliated cell sample, drain the supernatant, and assist in the production of cell agglomerates, and avoids the need for more frequent processing during the processing. The invention simplifies the operation steps, improves the processing efficiency of the exfoliated cell sample to a certain extent, and can roughly estimate the density of the exfoliated cell sample, which is helpful to judge whether the exfoliated cell sample is suitable for making cell agglomerates; the centrifuge tube body assembly comprises a centrifuge tube, an upper cover is threadedly connected to the outer periphery of the top of the centrifuge tube, a lower cover is threadedly connected to the outer periphery of the bottom of the centrifuge tube, a plurality of guide tubes are connected to the centrifuge tube, a screw cap is screwed to the outer periphery of the top of the guide tube, two elastic blocks are integrally formed on one side of the screw cap, and a limiting groove is provided on the upper cover corresponding to the screw cap Two limit blocks are arranged in the limit groove corresponding to the two elastic blocks. The two elastic blocks are pinched to gradually approach each other and gradually taken out from between the two limit blocks. This setting places the exfoliated cell sample into the centrifuge tube by opening the upper cover, closes the upper cover, places the centrifuge tube into the centrifuge for step-by-step centrifugal separation, and the exfoliated cell sample after sufficient centrifugal separation forms a supernatant and a precipitate. The screw cap of the guide tube of a suitable height is opened, and the supernatant is poured out of the centrifuge tube body through the guide tube, and then the upper cover and the lower cover are opened to push the cell mass from the lower tube mouth from top to bottom through the glass stirring rod. In this process, it is unnecessary to replace other devices, which simplifies the operation steps, improves the processing efficiency of the exfoliated cell sample to a certain extent, and can produce cell clumps with the same diameter, which is conducive to the subsequent preparation of standard staining slides; the density estimation component includes a placement groove corresponding to the bottom periphery of the centrifuge tube and arranged at the rear side of the centrifuge tube, and a density indicator label is detachably placed in the placement groove, and two density indicator lines are symmetrically arranged in the middle part of the density indicator label, and the density indicator label is composed of two rectangles with different gray values and two parallel horizontal lines, and the three primary colors (Red green blue, RGB) of the left rectangle are 75, 75, 75; the three primary colors (Red green blue, RGB) of the right rectangle are 200, 200, 200; the two rectangles are equal in size, and the RGB values of the two density indicator lines are 0, 0, 0. This setting places the density indicator label in the placement groove, and the label is observed through the exfoliated cell sample, so that the density can be effectively distinguished by visual inspection whether it is higher or lower than 10 5 / mL of liquid sample, calculated based on the volume of body fluid sample as 40mL, when the cell density is 5×10 5When the cell density is above 5×10 / mL, the density indicator line of the right rectangle is clear, and the device can be used to obtain cell clumps with the same diameter and a length of more than 1 mm for paraffin embedding in one step. However, when the cell density is less than 5×10 5 When the density indicator line of the right rectangle is blurred, pre-concentration is required before the device can be used to make cell agglomerates. The whole judgment process is simple and fast, which is conducive to quickly judging whether the exfoliated cell sample is suitable for making cell agglomerates.
[0015] 2. The centrifuge tube of the utility model is provided with a stirring assembly, which includes a stirring rod. The top of the stirring rod is clamped in the groove at the center of the bottom of the upper cover. A plurality of stirring blades are evenly arranged on the periphery of the stirring rod. A fixing ring is fixedly arranged at the bottom of the stirring rod through a plurality of connecting rods. The bottom periphery of the fixing ring is threadedly connected to the fixing groove at the bottom of the centrifuge tube. This arrangement is conducive to speeding up the centrifugal treatment of the exfoliated cell sample. At the same time, the overall detachable arrangement of the stirring assembly is conducive to its disassembly and cleaning.
[0016] 3. The left end of the outer side of the placement slot of the utility model is provided with an arc-shaped slot, which facilitates the placement and replacement of the density indicator label.
[0017] 4. The outer periphery of the top of the fixing ring of the utility model corresponds to the inner wall of the centrifugal tube and is integrally formed with a limiting ring. This setting can limit the installation of the stirring component to prevent it from slipping during the centrifugal separation process.
[0018] 5. In the present invention, a plurality of connecting rods are arranged in a circular array between the stirring rod and the fixing ring. This arrangement makes the connection between the stirring rod and the fixing ring more stable and the force is more uniform.
[0019] 6. In the utility model, a plurality of guide tubes are evenly distributed on the centrifuge tube, and the plurality of guide tubes are located in different horizontal planes, which facilitates the diversion and pouring of different liquid layers after separation. The guide tubes are made of high-performance polyolefin thermoplastic elastomer (TPE) material. Thermoplastic elastomer TPE (Thermoplastic Elastomer) is a material that has the characteristics of high elasticity, high strength, and high resilience of rubber and can be injection molded. It is environmentally friendly, non-toxic and safe, has a wide range of hardness, excellent colorability, soft touch, weather resistance, fatigue resistance and temperature resistance, excellent processing performance, no need for vulcanization, can be recycled to reduce costs, can be secondary injection molded and coated and bonded with base materials such as PP, PE, PC, PS, ABS, etc., or can be molded alone.
[0020] 7. The centrifuge tube, upper cover and lower cover of the utility model are all made of ABS material, which is acrylonitrile butadiene styrene (ABS), a commonly used thermoplastic resin material. It has the advantages of high strength, good toughness, and easy processing and molding. The processed product has a smooth surface and good mechanical properties. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 It is a front view cross-sectional structural schematic diagram of the utility model;
[0022] Figure 2 It is a rear view structural schematic diagram of the utility model;
[0023] Figure 3 It is a schematic diagram of the top view structure of the utility model;
[0024] Figure 4 It is a structural schematic diagram of the screw cap of the utility model;
[0025] Figure 5 for Figure 1 A schematic diagram of the enlarged structure of part A;
[0026] Figure 6 for Figure 1 A schematic diagram of the enlarged structure of part B;
[0027] Figure 7 for Figure 1 A schematic diagram of the enlarged structure of the middle C part;
[0028] Figure 8 for Figure 3 Schematic diagram of the enlarged structure of part D.
[0029] Figure markings: 1: centrifugal guide assembly, 2: density estimation assembly, 3: centrifuge tube, 4: upper cover, 5: lower cover, 6: guide tube, 7: screw cap, 8: elastic block, 9: limiting groove, 10: limiting block, 11: placement groove, 12: density indication label, 13: density indication line, 14: stirring assembly, 15: stirring rod, 16: slot, 17: stirring blade, 18: connecting rod, 19: fixing ring, 20: fixing groove, 21: arc groove, 22: limiting ring. DETAILED DESCRIPTION
[0030] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0031] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.
[0032] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "install", "connect", "connect", and "set" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0033] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8As shown, it includes a centrifugal flow guide component 1 and a density estimation component 2 arranged on the outer side of the lower part of the centrifugal flow guide component 1. This setting uses the centrifugal flow guide component 1 to centrifuge the exfoliated cell sample, drain the supernatant, and assist in the production of cell agglomerates, and uses the density estimation component 2 to roughly estimate the density of the exfoliated cell sample. In general, compared with the previous integrated device for cell fixation, density estimation and shaping, this device can sequentially perform centrifugal separation, drain the supernatant, and assist in the production of cell agglomerates on the exfoliated cell sample, and it is unnecessary to replace other devices during the processing, thereby simplifying the operation. The method comprises the steps of: improving the processing efficiency of the exfoliated cell sample to a certain extent, and roughly estimating the density of the exfoliated cell sample, which is helpful to judge whether the exfoliated cell sample is suitable for making cell agglomerates; the centrifuge tube 3 body assembly comprises a centrifuge tube 3, an upper cover 4 is threadedly connected to the outer periphery of the top of the centrifuge tube 3, a lower cover 5 is threadedly connected to the outer periphery of the bottom of the centrifuge tube 3, a plurality of flow guide tubes 6 are connected and arranged on the centrifuge tube 3, a screw cap 7 is screwed to the outer periphery of the top of the flow guide tube 6, two elastic blocks 8 are integrally formed on one side of the screw cap 7, a limiting groove 9 is provided on the upper cover 4 corresponding to the screw cap 7, and a limiting groove 9 is provided in the limiting groove 9 corresponding to the screw cap 7. The two elastic blocks 8 are provided with two limit blocks 10. The two elastic blocks 8 are pinched to gradually approach each other and gradually taken out from between the two limit blocks 10. In this setting, the exfoliated cell sample is placed in the centrifuge tube 3 by opening the upper cover 4, and the upper cover 4 is closed. The centrifuge tube 3 is placed in the centrifuge for step-by-step centrifugal separation. After sufficient centrifugal separation, the exfoliated cell sample forms a supernatant and a precipitate. The screw cap 7 of the guide tube 6 of a suitable height is opened, and the supernatant is poured out of the centrifuge tube 3 through the guide tube 6. Then, the upper cover 4 and the lower cover 5 are opened to push the cell agglomerates from the lower tube mouth from top to bottom through the glass stirring rod. This process The process is free from the need to replace other devices, which simplifies the operation steps, improves the processing efficiency of the exfoliated cell sample to a certain extent, and can produce cell clumps with the same diameter, which is conducive to the subsequent preparation of standard staining slides; the density estimation component 2 includes a placement groove 11 arranged at the rear side of the centrifuge tube 3 corresponding to the bottom periphery of the centrifuge tube 3, and a density indicator label 12 is detachably placed in the placement groove 11, and two density indicator lines 13 are symmetrically arranged in the middle part of the density indicator label 12, and the density indicator label 12 is composed of two rectangles with different gray values and two parallel horizontal lines. The three primary colors (Red green blue, RGB) of the left rectangle are 75, 75, 75; the three primary colors (Red green blue, RGB) of the right rectangle are 200, 200, 200; the two rectangles are equal in size, and the RGB values of the two density indicator lines 13 are 0, 0, 0. This setting places the density indicator label 12 in the placement groove 11, and the label is observed through the exfoliated cell sample, so that the density can be effectively distinguished by visual inspection. 5 / mL of liquid sample, calculated based on the volume of body fluid sample as 40mL, when the cell density is 5×105 When the cell density is above 5×10 / mL, the density indicator line 13 of the right rectangle is clear, and the device can be used to obtain cell clumps with the same diameter and a length of more than 1 mm for paraffin embedding in one step. However, when the cell density is less than 5×10 5 When the density indicator line 13 of the right rectangle is blurred, pre-concentration is required before the device can be used to make cell agglomerates. The whole judgment process is simple and fast, which is conducive to quickly judging whether the exfoliated cell sample is suitable for making cell agglomerates.
[0034] like Figure 1 , Figure 2 , Figure 6 and Figure 7 As shown, the centrifuge tube 3 is provided with a stirring assembly 14, and the stirring assembly 14 includes a stirring rod 15. The top of the stirring rod 15 is clamped in a clamping groove 16 at the center of the bottom of the upper cover 4. A plurality of stirring blades 17 are evenly arranged on the periphery of the stirring rod 15. A fixing ring 19 is fixedly arranged at the bottom of the stirring rod 15 through a plurality of connecting rods 18. The bottom periphery of the fixing ring 19 is threadedly connected to the fixing groove 20 at the bottom of the centrifuge tube 3. This arrangement is conducive to speeding up the centrifugal treatment of the exfoliated cell sample. At the same time, the overall detachable arrangement of the stirring assembly 14 is conducive to its disassembly and cleaning.
[0035] like Figure 1 and Figure 2 As shown, an arc-shaped groove 21 is provided at the left end of the outer groove body of the placement groove 11. This arrangement facilitates the placement and replacement of the density indication label 12.
[0036] like Figure 1 , Figure 2 and Figure 7 As shown, a limiting ring 22 is integrally formed on the periphery of the top of the fixing ring 19 corresponding to the inner wall of the centrifuge tube 3. This arrangement can limit the installation of the stirring assembly 14 to prevent it from slipping during the centrifugal separation process.
[0037] like Figure 1 and Figure 7 As shown, a plurality of connecting rods 18 are arranged in a ring array between the stirring rod 15 and the fixing ring 19. This arrangement makes the connection between the stirring rod 15 and the fixing ring 19 more stable and the force is more uniform.
[0038] like Figure 1 , Figure 2 , Figure 3 and Figure 5As shown, a plurality of flow guide tubes 6 are evenly distributed on the centrifuge tube 3, and the plurality of flow guide tubes 6 are located in different horizontal planes, which facilitates the diversion and pouring of different liquid layers after separation. The flow guide tubes 6 are made of high-performance polyolefin thermoplastic elastomer (TPE) material. Thermoplastic elastomer TPE (Thermoplastic Elastomer) is a material with high elasticity, high strength, high resilience of rubber, and the characteristics of being injection moldable. It is environmentally friendly, non-toxic and safe, has a wide range of hardness, excellent colorability, soft touch, weather resistance, fatigue resistance and temperature resistance, excellent processing performance, no need for vulcanization, can be recycled to reduce costs, can be secondary injection molded and coated and bonded with base materials such as PP, PE, PC, PS, ABS, etc., or can be molded alone.
[0039] like Figure 1 , Figure 2 , Figure 3 , Figure 5 , Figure 6 , Figure 7 and Figure 8 As shown, the centrifuge tube 3, the upper cover 4 and the lower cover 5 are all made of ABS material, which is acrylonitrile butadiene styrene (ABS), a commonly used thermoplastic resin material, a thermoplastic polymer material with high strength, good toughness, and easy processing and molding. The processed product has a smooth surface and good mechanical properties.
[0040] The specific operating principle is:
[0041] When using this device, you first need to put the density indicator label into the placement slot, open the upper cover and place the exfoliated cell sample into the centrifuge tube. By observing the label through the exfoliated cell sample, you can effectively distinguish between cells with a density higher or lower than 10 by visual inspection. 5 / mL of liquid sample, calculated based on the volume of body fluid sample as 40mL, when the cell density is 5×10 5 When the cell density is above 5×10 / mL, the density indicator line of the right rectangle is clear, and the device can be used to obtain cell clumps with the same diameter and a length of more than 1 mm for paraffin embedding in one step. However, when the cell density is less than 5×10 5 When the density of the exfoliated cell sample meets the formation standard of cell agglomerates, the upper cover is closed, and the centrifuge tube is placed in a centrifuge for step-by-step centrifugation. After sufficient centrifugation, the exfoliated cell sample forms a supernatant and a precipitate. The screw cap of the guide tube at a suitable height is opened, and the supernatant is poured out of the centrifuge tube body through the guide tube. Then, the upper and lower covers are opened, and the cell agglomerates are pushed out from the lower tube mouth from top to bottom by a glass stirring rod.
[0042] The electrical components appearing in this article can all be connected to the external main controller and 220V AC power through a transformer, and the main controller can be a conventional known device such as a computer that plays a control role. The conventional known device such as a computer that plays a control role can receive various data signals monitored by this device in real time. The electrical components provided by the utility model are only used for the technical solution according to the structural characteristics of the product. The product will be adjusted and modified after purchase to make it more matching and consistent with the technical solution of the utility model. It is a technical solution for the best application of the technical solution. The model of the product can be replaced and modified according to the required technical parameters. It is well known to technical personnel in this field. Therefore, technical personnel in this field can clearly obtain the corresponding use effect through the technical solution provided by the utility model.
[0043] The control method of the present invention is automatic control through a controller. The control circuit of the controller can be realized by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will not explain the control method and circuit connection in detail.
[0044] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the utility model.
Claims
1. A cell fixation, density estimation and shaping integrated device, characterized in that: It includes a centrifugal flow guide component and a density estimation component arranged on the outer side of the lower part of the centrifugal flow guide component; The centrifugal flow guide assembly comprises a centrifugal tube, an upper cover is threadedly connected to the outer periphery of the top of the centrifugal tube, a lower cover is threadedly connected to the outer periphery of the bottom of the centrifugal tube, a plurality of flow guide tubes are connected to the centrifugal tube, a screw cap is screwed to the outer periphery of the top of the flow guide tube, two elastic blocks are integrally formed on one side of the screw cap, a limiting groove is provided on the upper cover corresponding to the screw cap, and two limiting blocks are provided in the limiting groove corresponding to the two elastic blocks; The density estimation component comprises a placement groove arranged at the rear side of the centrifuge tube corresponding to the bottom periphery of the centrifuge tube, a density indication label is detachably placed in the placement groove, and two density indication lines are symmetrically arranged in the middle part of the density indication label.
2. A cell fixation, density estimation and shaping integrated device according to claim 1, characterized in that: The centrifuge tube is provided with a stirring assembly, which includes a stirring rod. The top of the stirring rod is clamped in a groove at the center of the bottom of the upper cover. A plurality of stirring blades are evenly arranged on the periphery of the stirring rod. A fixing ring is fixedly arranged on the bottom of the stirring rod through a plurality of connecting rods. The bottom periphery of the fixing ring is threadedly connected to the fixing groove at the bottom of the centrifuge tube accordingly.
3. The integrated device for cell fixation, density estimation and shaping according to claim 1, characterized in that: An arc groove is formed at the left end of the outer groove body of the placement groove.
4. The integrated device for cell fixation, density estimation and shaping according to claim 2, characterized in that: A limiting ring is integrally formed on the periphery of the top of the fixing ring corresponding to the inner wall of the centrifugal tube.
5. The integrated device for cell fixation, density estimation and shaping according to claim 2, characterized in that: A plurality of connecting rods are arranged in a ring array between the stirring rod and the fixing ring.
6. The integrated device for cell fixation, density estimation and shaping according to claim 1, characterized in that: The plurality of flow guide tubes are evenly distributed on the centrifugal tube, and the plurality of flow guide tubes are located in different horizontal planes. The flow guide tubes are made of high-performance polyolefin thermoplastic elastomer (TPE) material.
7. The integrated device for cell fixation, density estimation and shaping according to claim 1, characterized in that: The centrifuge tube, the upper cover and the lower cover are all made of ABS material.