Separating, screening and sucking device for cells

Abstract

The utility model relates to a separating, screening and sucking device for cells. The separating, screening and sucking device comprises a rotary sucking module, a separating and screening module, a visual detection module and a discharging and carrying module, the rotary suction module comprises a rotary manipulator module and a suction module arranged at the tail end of a mechanical arm of the rotary manipulator module; the separating and screening module comprises an objective table and an inverted microscope module arranged below the objective table; the visual detection module is used for guiding and positioning the rotary suction module and realizing displacement compensation; after the separating and screening module completes screening through vision, the rotary suction module sucks the microorganisms to the discharging and carrying module. Compared with manual cell selection, the efficiency of manual cell extraction can be improved; operations such as boring cell selection and easy errors are omitted, and the yield of manual cell selection is improved; and the device has strong universality, universality and use value in different fields and different products.

Description

Technical Field

[0001] The utility model relates to the technical field of visual detection, in particular to a cell separation, screening and absorption device. Background Art

[0002] Most traditional cell picking methods involve observing cells under a microscope and then manually aspirating the cells with a handheld aspiration device. However, manual picking methods usually have the following disadvantages:

[0003] 1. Operation accuracy and stability issues: Since the operation process needs to be performed manually, it involves the skills and experience of the operator. There may be differences in operation accuracy and stability between different personnel, which may lead to inaccurate or unstable cell aspiration. In addition, long-term manual operation may cause fatigue, further affecting the accuracy of the operation.

[0004] 2. Efficiency issue: Manual handheld aspiration of cells is usually a relatively slow process, especially when the amount or position of aspiration needs to be precisely controlled. This inefficiency may not be suitable for experiments or application scenarios that require rapid processing of a large number of samples.

[0005] 3. Risk of cross contamination: In the process of aspirating cells, if the operation is improper or the equipment is not cleaned thoroughly, cross contamination between samples may occur. This contamination may not only affect the accuracy of the experimental results, but also cause irreversible effects on the research.

[0006] 4. Potential damage to cells: Due to the limitation of manual operation, it may not be possible to accurately control the suction force and speed, which may cause damage or destruction to cells. For experiments with high requirements on cell status, this damage may be fatal.

[0007] 5. Poor repeatability and consistency: Due to the variability and uncertainty of manual operation, the results of cell aspiration using manual handheld aspiration devices may perform poorly in terms of repeatability and consistency. This is an important issue for studies that require accurate comparison of different samples or experimental conditions. Utility Model Content

[0008] The technical problem to be solved by the utility model is to provide a cell separation, screening and absorption device, which has the characteristics of good continuity, stability and high precision.

[0009] The technical solution adopted by the utility model to solve its technical problems is: a cell separation, screening and suction device, including a rotary suction module, a separation and screening module, a visual detection module and a material unloading and handling module; the rotary suction module includes a rotary manipulator module and a suction module arranged at the end of the mechanical arm of the rotary manipulator module; the separation and screening module includes a stage and an inverted microscope module arranged below the stage; the visual detection module guides and positions the rotary suction module and realizes displacement compensation; after the separation and screening module completes the screening by vision, the rotary suction module absorbs the microorganisms into the material unloading and handling module.

[0010] Furthermore, the rotary manipulator module described in the utility model includes a DD motor as the R-axis; the DD motor is provided with a grinding screw assembly as the Y-axis, and the grinding screw assembly as the Y-axis is provided with a grinding screw assembly as the Z-axis; the suction module is arranged at the end of the grinding screw assembly as the Z-axis.

[0011] Furthermore, the suction module described in the present invention includes an extraction needle, and the extraction needle is connected to the plunger pump and the peristaltic pump respectively through a Luer connector and a pump adapter.

[0012] Furthermore, the loading platform of the present invention comprises an XY electric module, a culture dish is placed on the loading platform, and the culture dish is moved by the XY electric module.

[0013] Furthermore, a constant temperature device is also provided on the loading platform of the utility model.

[0014] Furthermore, the visual inspection module described in the utility model includes a frame, a mounting plate is arranged on the top of the frame, a Z-axis imaging inspection module is arranged on the mounting plate, and an XY-axis imaging inspection module is arranged below the mounting plate.

[0015] Furthermore, the material unloading and handling module described in the utility model includes a screw module, a carrier plate is provided on the screw module, and a secondary positioning assembly is provided on the carrier plate; the secondary positioning assembly includes a Y-shaped push block, the Y-shaped push block is provided at a top corner position of the carrier plate, and a sliding module is provided at the bottom of the Y-shaped push block.

[0016] Furthermore, the rotary suction module, separation and screening module, visual inspection module and material unloading and handling module described in the utility model are arranged on a marble bottom plate.

[0017] The beneficial effect of the utility model is that it solves the defects existing in the background technology. Compared with manual operation of selecting cells, the utility model can screen and mark all cells in the well plate at one time, confirm the cells to be selected by an inverted microscope, and extract the cells into the culture dish in batches, thereby improving the efficiency of manual cell extraction; compared with manual operation of selecting cells, some areas are repeatedly selected, and cells in some areas are missed, the utility model can save the boring and error-prone operations of selecting cells, and improve the yield rate of manual cell selection; the utility model has strong versatility, extensiveness and use value in different fields and different products. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a top view of the structure of the utility model;

[0019] Figure 2 It is a structural schematic diagram of the separation and screening module of the utility model;

[0020] Figure 3 It is a structural schematic diagram of the visual inspection module and the material handling module of the utility model;

[0021] In the figure: 1. Rotating suction module; 2. Separation and screening module; 3. Visual inspection module; 4. Unloading and handling module; 5. Marble bottom plate;

[0022] 21. Stage; 22. Inverted microscope module; 23. XY motor module; 24. Culture dish; 25. Constant temperature device;

[0023] 31. Rack; 32. Mounting plate; 33. Z-axis imaging detection module; 34. XY-axis imaging detection module;

[0024] 41. Screw module; 42. Carrier plate; 43. Y-type push block. DETAILED DESCRIPTION

[0025] Now the utility model is further described in detail in conjunction with the accompanying drawings and preferred embodiments. These drawings are simplified schematic diagrams, which only illustrate the basic structure of the utility model in a schematic manner, and therefore only show the components related to the utility model.

[0026] like Figure 1-Figure 3 The cell separation, screening and suction device shown is applicable to cells or bacteria and other similar microbial monomers; it includes a rotary suction module 1, a separation and screening module 2, a visual inspection module 3 and a material handling module 4 arranged on a marble bottom plate 5; the device has very high precision, and the overall repeatability can reach ±0.005MM, which can more accurately absorb cells. The bottom plate of the device is made of marble, which has high processing precision, small deformation, and can prevent the machine from shaking, thereby ensuring the detection accuracy.

[0027] The rotating suction module 1 includes a rotating robot module and a suction module arranged at the end of the robot arm of the rotating robot module; the rotating robot module includes a DD motor as the R axis; the DD motor is provided with a grinding screw assembly as the Y axis, and the grinding screw assembly as the Y axis is provided with a grinding screw assembly as the Z axis; the suction module is arranged at the end of the grinding screw assembly as the Z axis.

[0028] Using high-precision ground screw modules and DD motors, the single-axis repeatability is ±0.0025mm.

[0029] The suction module includes an extraction needle, which is connected to a plunger pump and a peristaltic pump through a Luer connector and a pump adapter. The suction device used combines an inexpensive plunger pump with a peristaltic pump to reduce costs while ensuring micro-aspiration in a stable manner. The suction device controls the cell extraction needle through a plunger pump. In order to remove air from the cell needle and the pipeline, another peristaltic pump is used to fill the liquid into the plunger pump and the pipeline. After the pipeline is filled with liquid, the valve is closed to isolate the peristaltic pump from the plunger pump liquid and prevent the liquid in the plunger pump from flowing back.

[0030] The separation and screening module 2 includes a stage 21 and an inverted microscope module 22 disposed below the stage; the stage includes an XY electric module 23, a culture dish 24 is placed on the stage, and the culture dish is moved by the XY electric module. A constant temperature device 25 is also disposed on the stage.

[0031] The inverted microscope used has an XY stage, which can center the cells to be absorbed directly above the camera. There is a switch between bright field and fluorescence mode. In the bright field state, the light source is turned on; in the fluorescence state, the light source is turned off. It can be applied to a variety of scenarios. It can switch between 4x, 10x, 20x, and 40x microscopes to observe cells under multiple microscopes. The rotatable light source can avoid absorbing cell structures and save equipment space. The constant temperature table that can maintain the temperature of the cells keeps the temperature at room temperature and the cells remain active.

[0032] The visual inspection module 3 guides and positions the rotating suction module and realizes displacement compensation; the visual inspection module includes a frame 31, a mounting plate 32 is arranged on the top of the frame, a Z-axis imaging detection module 33 is arranged on the mounting plate, and an XY-axis imaging detection module 34 is arranged below the mounting plate. After the cell extraction needle is manually installed, the visual guidance station needs to be used to guide and position the needle height and position, and realize displacement compensation. The XY-axis imaging detection module detects the X and Y-axis coordinates of the needle; the Z-axis imaging detection module detects the Z-axis height of the needle.

[0033] After the separation and screening module completes the screening through vision, the rotary suction module absorbs the microorganisms into the material unloading and transporting module. The material unloading and transporting module 4 includes a screw module 41. After the cell absorption is completed, the screw module drives the culture dish to move to the bottom of the cell needle to receive the cells. A carrier plate 42 is provided on the screw module, and a secondary positioning component is provided on the carrier plate; the secondary positioning component includes a Y-shaped push block 43, and the Y-shaped push block 43 is provided at a top corner position of the carrier plate, and a sliding module is provided at the bottom of the Y-shaped push block. After the culture dish for unloading is placed on the carrier plate, the Y-shaped push block can be pushed forward from the top corner position by the sliding module provided at the bottom to realize the secondary positioning of the culture dish.

[0034] The action process is as follows: Step 1: manually place the culture dish A to be screened on the stage; Step 2: manually place the culture dish B to the secondary positioning; Step 3: manually install the cell extraction needle; Step 4: the electric stage moves through the XY axis, and the required cells are selected by vision; Step 5: the cell extraction needle moves to the visual detection calibration coordinates through the RZY three-axis manipulator; Step 6: the cell extraction needle passes through the RZY three-axis manipulator to the culture dish A to absorb the screened cells; Step 7: after absorbing the cells, put the cells into the culture dish B; Step 8: after completing the cell selection of all target cells, it is prompted to manually take away the culture dish B.

[0035] The utility model takes pictures through an inverted microscope camera, the electric stage moves through the XY axis, and uses vision to classify and mark cells. The XYZ coordinates of the cells are obtained through an algorithm. The RZY three-axis manipulator module moves to the position according to the known coordinates, and then the cells are micro-absorbed into the needle tip through a plunger pump. After the target cells are absorbed, they are placed in a culture dish. Through the three-axis linkage technology, continuous, stable, high-precision and rapid identification, positioning and numbering are achieved according to morphological indicators such as microbial cell length, width, aspect ratio, regularity, etc., and the single microbial cells of the target morphology are automatically extracted. The cells in the sample can be separated into single cells, while other traditional biological separation technologies usually separate them in cell groups. This enables single cell sorting technology to study and analyze the characteristics and functions of single cells, and reveal the differences between cell heterogeneity and individual cells. Single cell sorting technology has the characteristics of high precision and high throughput. It can quickly and accurately separate a large number of cells, so that scientific researchers can have a more comprehensive understanding of the diversity and complexity of cells. Compared with traditional cell separation technology, single cell sorting technology can better capture rare cell subpopulations and improve the resolution of samples.

[0036] The above description only describes the specific implementation methods of the utility model. Various examples do not limit the essential content of the utility model. Ordinary technicians in the relevant technical field can modify or deform the specific implementation methods described above after reading the description without departing from the essence and scope of the utility model.

Claims

1. A cell separation, screening and aspiration device, characterized in that: It includes a rotary suction module, a separation and screening module, a visual inspection module and a material unloading and handling module; the rotary suction module includes a rotary manipulator module and a suction module arranged at the end of the mechanical arm of the rotary manipulator module; the separation and screening module includes a stage and an inverted microscope module arranged below the stage; the visual inspection module guides and positions the rotary suction module and realizes displacement compensation; after the separation and screening module completes the screening through vision, the rotary suction module absorbs the microorganisms to the material unloading and handling module.

2. A cell separation, screening and aspiration device as claimed in claim 1, characterized in that: The rotary manipulator module includes a DD motor as an R-axis; a grinding screw assembly as a Y-axis is arranged on the DD motor, and a grinding screw assembly as a Z-axis is arranged on the grinding screw assembly as the Y-axis; The suction module is arranged at the end of the grinding screw assembly serving as the Z axis.

3. The cell separation, screening and aspiration device according to claim 1, characterized in that: The suction module comprises an extraction needle, and the extraction needle is connected to a plunger pump and a peristaltic pump respectively through a Luer connector and a pump adapter.

4. The cell separation, screening and aspiration device according to claim 1, characterized in that: The stage comprises an XY electric module, a culture dish is placed on the stage, and the culture dish is moved by the XY electric module.

5. A cell separation, screening and aspiration device as claimed in claim 4, characterized in that: The loading platform is also provided with a constant temperature device.

6. The cell separation, screening and aspiration device according to claim 1, characterized in that: The visual inspection module comprises a frame, a mounting plate is arranged on the top of the frame, a Z-axis imaging inspection module is arranged on the mounting plate, and an XY-axis imaging inspection module is arranged below the mounting plate.

7. The cell separation, screening and aspiration device according to claim 1, characterized in that: The material unloading and handling module includes a screw module, a carrier plate is arranged on the screw module, and a secondary positioning component is arranged on the carrier plate; the secondary positioning component includes a Y-shaped push block, the Y-shaped push block is arranged at a top corner position of the carrier plate, and a sliding module is arranged at the bottom of the Y-shaped push block.

8. The cell separation, screening and aspiration device according to claim 1, characterized in that: The rotating suction module, the separation and screening module, the visual inspection module and the material unloading and handling module are arranged on the marble bottom plate.

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