Cell culture fluid circulating device

Abstract

The utility model discloses a cell culture fluid circulating device which comprises a base, the upper end of the base is fixedly connected with a U-shaped plate, a bottle body is placed at the upper end of the base, one side of the bottle body is fixedly connected with a fluid inlet pipe, and the other side of the bottle body is fixedly connected with a fluid outlet pipe. The upper end of the liquid inlet pipe and the upper end of the liquid outlet pipe are both in threaded connection with pipe caps, a bottle cap is arranged at the upper end of the bottle body, the upper end of the base is rotationally connected with a threaded rod, the upper end of the base is fixedly connected with a guide rod, the threaded rod and the guide rod are both provided with moving blocks, one moving block is in threaded connection with the threaded rod, and the other moving block is in threaded connection with the guide rod. And the other moving block is connected with the guide rod in a sliding manner. Through cooperation of a threaded rod, a guide rod, a moving block, a clamping plate and other structures, the bottle body can move up and down, a plurality of stirring rods are matched to stir a culture solution, so that the culture solution circularly flows in the bottle body, and cell adherence growth is effectively avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture devices, in particular to a cell culture fluid circulation device. Background Art

[0002] Cell culture medium is a general term for the medium used for tissue or cell culture. In cell culture technology, there is a suspension culture method. Suspension culture refers to a tissue culture system that cultivates single cells and small cell clusters in a liquid culture medium that is constantly stirred or shaken. It is a culture method for non-anchorage-dependent cells.

[0003] However, when using cell culture flasks for suspension culture, the cell culture fluid is stirred by a stirring shaft so that it can circulate. However, with this stirring method, the stirring shaft only stirs in the horizontal direction, and the cell culture fluid is unevenly mixed and has poor fluidity, resulting in some cells growing on the wall. Therefore, we need to consider how to solve this problem. Utility Model Content

[0004] The purpose of the utility model is to solve the shortcomings of the prior art and to propose a cell culture fluid circulation device, which enables the bottle body to move up and down through the coordination of structures such as threaded rods, guide rods, moving blocks and clamps, and cooperates with multiple stirring rods to stir the culture fluid so that the culture fluid circulates in the bottle body, effectively preventing cells from growing on the wall.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A cell culture fluid circulation device comprises a base, the upper end of the base is fixedly connected to a U-shaped plate, a bottle body is placed on the upper end of the base, a liquid inlet pipe is fixedly connected to one side of the bottle body, a liquid outlet pipe is fixedly connected to the other side of the bottle body, the upper ends of the liquid inlet pipe and the liquid outlet pipe are both threadedly connected to tube covers, the upper end of the bottle body is provided with a bottle cover, the upper end of the base is rotatably connected to a threaded rod, the upper end of the base is fixedly connected to a guide rod, and moving blocks are provided on the threaded rod and the guide rod, one of the moving blocks is threadedly connected to the threaded rod, and the other of the moving blocks is slidably connected to the guide rod. Then, electric push rods are installed on opposite sides of the two moving blocks, and the telescopic ends of the two electric push rods are fixedly connected with clamps, the upper end of the U-shaped plate is rotatably connected with a rotating shaft, the lower end of the rotating shaft penetrates the U-shaped plate and is fixedly connected with a stirring shaft, the lower end of the stirring shaft extends into the inside of the bottle body, and the part of the stirring shaft located in the bottle body is fixedly connected with a plurality of stirring rods, a motor is installed on the upper end of the U-shaped plate, the output shaft end of the motor penetrates the U-shaped plate and is fixedly connected to the upper end of the threaded rod, and the output shaft of the motor is connected to the part of the rotating shaft located at the upper end of the U-shaped plate through a transmission structure.

[0007] Preferably, the transmission structure includes two transmission wheels, which are respectively mounted on the output shaft of the motor and the part of the rotating shaft located at the upper end of the U-shaped plate, and the two transmission wheels are connected by belt transmission.

[0008] Preferably, an air pump is installed at the upper end of the U-shaped plate, and the air outlet end of the air pump is connected to the interior of the bottle body through an air outlet pipe.

[0009] Preferably, one end of the air outlet pipe is arranged in a ring shape inside the bottle body, and a plurality of air outlets are opened at the lower end of the ring-shaped part of the air outlet pipe.

[0010] Preferably, protective layers are provided on opposite sides of the two clamping plates, and the two protective layers are made of rubber material.

[0011] Preferably, a plurality of anti-slip grooves are provided at the lower end of the base, and the plurality of anti-slip grooves are all triangular in shape.

[0012] Compared with the prior art, the utility model has the following beneficial effects:

[0013] 1. A rotating shaft, a stirring shaft, a stirring rod and other structures are provided. The rotating shaft drives the stirring shaft to rotate, and then drives the multiple stirring rods to rotate, so as to stir the cell culture medium in the bottle body to make it circulate and prevent the cells from growing on the wall;

[0014] 2. The threaded rod, moving block and clamping plate are provided so that the bottle can move up and down to disturb the cell culture solution, and multiple stirring rods can move vertically, further increasing the stirring range and fluidity to achieve better circulation of the culture solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a structural schematic diagram of a cell culture fluid circulation device proposed by the utility model;

[0016] Figure 2 for Figure 1 Schematic cross-sectional view of ;

[0017] Figure 3 for Figure 1 Schematic top view of .

[0018] In the figure: 1 base, 2 U-shaped plate, 3 bottle body, 4 liquid inlet pipe, 5 liquid outlet pipe, 6 tube cover, 7 bottle cover, 8 threaded rod, 9 guide rod, 10 moving block, 11 electric push rod, 12 clamping plate, 13 rotating shaft, 14 stirring shaft, 15 stirring rod, 16 motor, 17 transmission wheel, 18 belt, 19 air pump, 20 air outlet pipe, 21 air outlet. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0020] Reference Figure 1-Figure 3 A cell culture fluid circulation device comprises a base 1, a plurality of anti-skid patterns are arranged at the lower end of the base 1, and the plurality of anti-skid patterns are triangular in shape, a U-shaped plate 2 is fixedly connected to the upper end of the base 1, a bottle body 3 is placed at the upper end of the base 1, a liquid inlet pipe 4 is fixedly connected to one side of the bottle body 3, a liquid outlet pipe 5 is fixedly connected to the other side of the bottle body 3, a tube cover 6 is threadedly connected to the upper ends of the liquid inlet pipe 4 and the liquid outlet pipe 5, a bottle cover 7 is arranged at the upper end of the bottle body 3, the bottle cover 7 is composed of two cover plates, and the two cover plates are mutually matched and sleeved on the upper end of the bottle body 3, a threaded rod 8 is rotatably connected to the upper end of the base 1, a guide rod 9 is fixedly connected to the upper end of the base 1, and moving blocks 10 are arranged on the threaded rod 8 and the guide rod 9, one of the moving blocks 10 is threadedly connected to the threaded rod 8, and the other moving block 10 is slidably connected to the guide rod 9, and electric push rods 11 are installed on the opposite sides of the two moving blocks 10;

[0021] The two clamping plates 12 are fixedly connected to the telescopic ends of the two electric push rods 11, and the opposite sides of the two clamping plates 12 are provided with protective layers, and the two protective layers are made of rubber material. The two clamping plates 12 are arc-shaped plates, and the two clamping plates 12 cooperate with each other to clamp and fix the bottle body 3. The upper end of the U-shaped plate 2 is rotatably connected with a rotating shaft 13, and the lower end of the rotating shaft 13 passes through the U-shaped plate 2 and is fixedly connected with a stirring shaft 14. The stirring shaft 14 can be removed. The two clamping plates 12 are separated by starting the two electric push rods 11 to release the limit of the bottle body 3, and then the bottle cap 7 is opened. After taking out the stirring shaft 14, the bottle body 3 can be taken out for replacement or cleaning. The lower end of the stirring shaft 14 extends into the inside of the bottle body 3, and the part of the stirring shaft 14 located in the bottle body 3 is fixedly connected with a plurality of stirring rods 15. A motor 16 is installed on the upper end of the U-shaped plate 2, and the motor 16 is a servo motor. The end of the output shaft of the motor 16 passes through the U-shaped plate 2 and is fixedly connected to the upper end of the threaded rod 8.

[0022] The output shaft of the motor 16 is connected to the part of the rotating shaft 13 located at the upper end of the U-shaped plate 2 through a transmission structure. The transmission structure includes two transmission wheels 17. The two transmission wheels 17 are respectively installed on the output shaft of the motor 16 and the part of the rotating shaft 13 located at the upper end of the U-shaped plate 2. The two transmission wheels 17 are connected through a belt 18. By rotating the rotating shaft 13, the stirring shaft 14 brings a plurality of stirring rods 15 to stir the cell culture liquid inside the bottle body 3, so that it circulates and prevents the cells from growing on the wall. In this process, the threaded rod 8 will also rotate, thereby being clamped and fixed by the two clamps 12. The bottle body 3 moves up and down, so that the multiple stirring rods 15 move in the vertical direction, which further increases the stirring range, makes the cell culture fluid circulate better, and avoids the cell wall growth. An air pump 19 is installed on the upper end of the U-shaped plate 2. The air outlet end of the air pump 19 is connected with the inside of the bottle body 3 through an air outlet pipe 20. One end of the air outlet pipe 20 is annularly arranged inside the bottle body 3. A plurality of air outlets 21 are opened at the lower end of the annular part of the air outlet pipe 20. The gas required for cell culture is introduced into the bottle body 3 through the air pump 19, and the gas can fully contact with the cell culture fluid by setting the annular tube.

[0023] In the utility model, when culturing cells, a culture solution is injected into the bottle body 3, and then the motor 16 and the air pump 19 are started. After the motor 16 is started, the drive wheel 17 and the belt 18 drive the rotating shaft 13 to rotate, and then the stirring shaft 14 drives the plurality of stirring rods 15 to rotate, stirring the cell culture solution inside the bottle body 3, so that the cell culture solution circulates in the bottle body 3, thereby preventing the cells from growing on the wall;

[0024] In the above process, after the motor 16 is started, it also drives the threaded rod 8 to rotate. With the cooperation of the guide rod 9, the two moving blocks 10 move. The two moving blocks 10 move, so that the two electric push rods 11 drive the corresponding clamping plates 12 to move, and then the bottle body 3 clamped by the two clamping plates 12 moves. By moving the bottle body 3 up and down, the cell culture liquid inside the bottle body 3 can be disturbed, and the multiple stirring rods 15 can move in the vertical direction, which further increases the stirring range and makes the cell culture liquid circulate better.

[0025] After the air pump 19 is started, gas is introduced into the bottle body 3 through the air outlet pipe 20. Since one end of the air outlet pipe 20 is arranged in a ring shape inside the bottle body 3, the gas enters the culture solution through multiple air outlets 21 at the lower end of the ring part, so that the gas can fully contact the culture solution.

[0026] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell culture fluid circulation device, comprising a base (1), characterized in that: The upper end of the base (1) is fixedly connected to a U-shaped plate (2), a bottle body (3) is placed on the upper end of the base (1), a liquid inlet pipe (4) is fixedly connected to one side of the bottle body (3), a liquid outlet pipe (5) is fixedly connected to the other side of the bottle body (3), the upper ends of the liquid inlet pipe (4) and the liquid outlet pipe (5) are both threadedly connected to pipe covers (6), and a bottle cover (7) is provided at the upper end of the bottle body (3); The upper end of the base (1) is rotatably connected to a threaded rod (8), the upper end of the base (1) is fixedly connected to a guide rod (9), the threaded rod (8) and the guide rod (9) are both provided with moving blocks (10), one of the moving blocks (10) is threadedly connected to the threaded rod (8), the other of the moving blocks (10) is slidably connected to the guide rod (9), electric push rods (11) are installed on opposite sides of the two moving blocks (10), and the telescopic ends of the two electric push rods (11) are fixedly connected to clamping plates (12); The upper end of the U-shaped plate (2) is rotatably connected to a rotating shaft (13); the lower end of the rotating shaft (13) passes through the U-shaped plate (2) and is fixedly connected to a stirring shaft (14); the lower end of the stirring shaft (14) extends into the interior of the bottle body (3); the portion of the stirring shaft (14) located inside the bottle body (3) is fixedly connected to a plurality of stirring rods (15); a motor (16) is mounted on the upper end of the U-shaped plate (2); the output shaft end of the motor (16) passes through the U-shaped plate (2) and is fixedly connected to the upper end of the threaded rod (8); The output shaft of the motor (16) is connected to the portion of the rotating shaft (13) located at the upper end of the U-shaped plate (2) through a transmission structure.

2. A cell culture fluid circulation device according to claim 1, characterized in that: The transmission structure comprises two transmission wheels (17), the two transmission wheels (17) being respectively mounted on the output shaft of the motor (16) and the portion of the rotating shaft (13) located at the upper end of the U-shaped plate (2), and the two transmission wheels (17) being connected by a belt (18).

3. A cell culture fluid circulation device according to claim 1, characterized in that: An air pump (19) is installed at the upper end of the U-shaped plate (2), and an air outlet end of the air pump (19) is connected to the interior of the bottle body (3) through an air outlet pipe (20).

4. A cell culture fluid circulation device according to claim 3, characterized in that: One end of the air outlet pipe (20) is arranged in a ring shape inside the bottle body (3), and a plurality of air outlets (21) are provided at the lower end of the ring-shaped portion of the air outlet pipe (20).

5. The cell culture fluid circulation device according to claim 1, characterized in that: Opposite sides of the two clamping plates (12) are provided with protective layers, and the two protective layers are made of rubber material.

6. A cell culture fluid circulation device according to claim 1, characterized in that: The lower end of the base (1) is provided with a plurality of anti-slip grooves, and the plurality of anti-slip grooves are all triangular in shape.

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