Device for inducing blood microcirculation disturbance of zebra fish

Abstract

The utility model discloses a device for inducing blood microcirculation disturbance of zebra fish. The device comprises a water tank for storing the zebra fish; the water circulation filtering assembly is arranged on the outer side of the water tank; water in the water tank flows through the circulating pipelines and the filtering frame, scrap iron in the filtering frame reacts with oxygen, the purpose of consuming the oxygen in the water is achieved, then the vacuum pump vacuumizes gas on the upper layer of an inner cavity of the water tank, and the water circulating filtering assembly is arranged in the water tank and comprises circulating pipelines installed on the two sides of the water tank. The oxygen in the water in the water tank is promoted to be separated out of the water, the content of the oxygen in the water is reduced, then the water tank is heated through the temperature control equipment, the oxygen separation speed in the water can be increased, the reaction speed of iron blood and the oxygen is increased, and after the oxygen content in the water is reduced, zebra fish cannot breathe oxygen in the water, so that the oxygen content in the blood is reduced; according to the method, chemical agents do not need to be put, and the method is safe and easy to operate.

Description

Technical Field

[0001] The utility model relates to the technical field of blood circulation, in particular to a device for inducing blood microcirculation disorder in zebrafish. Background Art

[0002] Microcirculation refers to the blood circulation between arterioles and venules. The most fundamental function of blood circulation is to exchange substances between blood and tissues, and this function is achieved in the microcirculation part. Microcirculation is crucial to maintaining human health, promoting wound healing, regulating body temperature, and skin color and appearance.

[0003] Using zebrafish to study microcirculation takes advantage of the high homology between zebrafish embryos and human genomes and the transparent nature of fish embryos, which makes it easy to observe blood vessels. To study and improve microcirculation, it is necessary to first establish a model of microcirculatory disorders. Current experiments generally use direct comparison with a blank group or by adding the chemical arachidonic acid to induce thrombosis in zebrafish.

[0004] The first method does not establish a model of microcirculatory disorders, but uses a blank comparison method to verify the effect of improving microcirculation. However, the microcirculation of zebrafish in the blank group is easily changed by external stimuli, such as rising ambient temperature or stress, which will affect the results of improving microcirculation. Although the effect of establishing a thrombosis model by using arachidonic acid is better, it is necessary to inject this substance into the circulatory system of zebrafish, which has the problem of difficulty in operation. In addition, arachidonic acid has certain irritation and toxicity, which will have adverse effects on both the experimenter and the zebrafish. Utility Model Content

[0005] The utility model aims to provide a device for inducing blood microcirculation disorder in zebrafish, which can reduce the oxygen content in water, thereby inducing blood circulation disorder in zebrafish, so as to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solution: a device for inducing blood microcirculation disorder in zebrafish, comprising:

[0007] tanks for holding zebrafish;

[0008] A water circulation filter assembly disposed outside the water tank;

[0009] The water circulation filter assembly comprises a circulation pipe installed on both sides of the water tank, a filter frame is fixedly connected to the middle section of the circulation pipe, and the inner cavity of the filter frame is filled with iron filings;

[0010] A vacuum assembly disposed outside the water tank;

[0011] The vacuum assembly comprises a tank cover installed on the top of the water tank, an air guide tube is installed on the top of the tank cover, and one end of the air guide tube away from the tank cover is connected to a vacuum pump;

[0012] Also included is a temperature control device located at the bottom of the sink.

[0013] Preferably, a sealing cover is installed on the top of the filter frame, a filter mesh frame is installed on the bottom of the sealing cover, and the iron filings are located in the filter mesh frame.

[0014] Preferably, two groups of guide plates are installed in the filter frame, the guide plates are arranged in a staggered manner, and the outer side of the filter mesh frame is attached to the inner wall of the filter frame and the outer wall of the guide plates.

[0015] Preferably, both ends of the water tank are connected with right-angle elbows, the end of the circulation pipe away from the filter frame is inserted into the inside of the right-angle elbow, and the outer side of the circulation pipe is provided with a first tightening threaded sleeve, which is threadedly connected to the end of the right-angle elbow away from the water tank.

[0016] Preferably, the top of the slot cover is connected to a linear joint, the end of the air guide tube away from the vacuum pump is inserted into the interior of the linear joint, the outer side of the air guide tube is provided with a second tightening threaded sleeve, and the second tightening threaded sleeve is threadedly connected to the end of the linear joint away from the slot cover.

[0017] Preferably, the water circulation filter assembly further comprises a water pump, the water pump is installed on the circulation pipeline, and a control valve is installed in the right-angle elbow.

[0018] Preferably, a plurality of mounting holes are provided on the outer side of the sealing cover, and mounting screws are movably arranged in the mounting holes, and one end of the mounting screw passes through the sealing cover and is threadedly connected to the filter frame.

[0019] Preferably, the inner cavity of the tank cover is provided with a groove, and the groove is filled with a sealing ring, and the sealing ring passes through the groove and is pressed and fitted with the outer wall of the tank.

[0020] Preferably, filter plates are installed on both sides of the inner cavity of the water tank, and the filter plates are located on the outer side of the end of the circulation pipeline.

[0021] Preferably, a support seat is provided at the bottom of the temperature control device, a slot for engaging with the vacuum pump and the temperature control device is provided at the top of the support seat, and a placement rack that fits the water pump and the bottom of the filter frame is fixed to the top of the support seat.

[0022] Compared with the prior art, the beneficial effects of the utility model are:

[0023] 1. The utility model consumes oxygen in the water by passing the water in the water tank through a circulation pipe and a filter frame, and utilizing the iron filings in the filter frame to react with oxygen, and then a vacuum pump evacuates the gas in the upper layer of the inner cavity of the water tank, so as to promote the precipitation of oxygen in the water in the water tank and reduce the oxygen content in the water, and then a temperature control device is used to heat the water tank, which can not only accelerate the precipitation speed of oxygen in the water, but also improve the reaction speed of iron blood and oxygen. When the oxygen content in the water is reduced, the zebrafish cannot breathe oxygen in the water, resulting in a decrease in the oxygen content in the blood, thereby inducing blood circulation disorders in the zebrafish. This method does not require the use of chemical agents and is safe and easy to operate.

[0024] 2. The utility model prevents zebrafish from entering the circulation pipe and causing blockage of the circulation pipe by installing two sets of filter plates in the water tank. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0026] Figure 2 It is a schematic diagram of the side cross-sectional three-dimensional structure of the slot cover of the utility model;

[0027] Figure 3 It is a partial three-dimensional structural schematic diagram of the utility model;

[0028] Figure 4 It is a schematic diagram of the split three-dimensional structure of the circulation pipeline and the right-angle elbow of the utility model;

[0029] Figure 5 It is a schematic diagram of the exploded three-dimensional structure of the sealing cover and the filter frame of the utility model;

[0030] Figure 6 It is a three-dimensional structural schematic diagram of the support seat of the utility model.

[0031] Numbers in the figure: 1. water tank; 2. water circulation filter assembly; 21. circulation pipe; 22. filter frame; 23. iron filings; 24. water pump; 3. vacuum assembly; 31. tank cover; 32. air guide pipe; 33. vacuum pump; 4. temperature control equipment; 5. sealing cover; 6. filter frame; 7. guide plate; 8. right-angle elbow; 9. first tightening threaded sleeve; 10. straight joint; 11. second tightening threaded sleeve; 12. control valve; 13. mounting screws; 14. sealing ring; 15. filter plate; 16. support seat; 17. slot; 18. placement rack. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0033] The utility model provides Figures 1 to 6 The device shown in the figure for inducing blood microcirculation disorder in zebrafish comprises: a water tank 1 for storing zebrafish; a water circulation filter assembly 2 arranged outside the water tank 1;

[0034] The water circulation filter assembly 2 comprises a circulation pipe 21 installed on both sides of the water tank 1, a filter frame 22 is fixedly connected to the middle section of the circulation pipe 21, and the inner cavity of the filter frame 22 is filled with iron filings 23;

[0035] A vacuum pumping assembly 3 is arranged outside the water tank 1; the vacuum pumping assembly 3 includes a tank cover 31 installed on the top of the water tank 1, an air guide pipe 32 is installed on the top of the tank cover 31, and one end of the air guide pipe 32 away from the tank cover 31 is connected to a vacuum pump 33;

[0036] It also includes a temperature control device 4 located at the bottom of the water tank 1; by passing the water in the water tank 1 through the circulation pipe 21 and the filter frame 22, the iron filings 23 in the filter frame 22 react with oxygen to achieve the purpose of consuming oxygen in the water, and then the vacuum pump 33 evacuates the gas in the upper layer of the inner cavity of the water tank 1, so as to promote the oxygen in the water in the water tank 1 to precipitate from the water, thereby reducing the oxygen content in the water, and then the temperature control device 4 is used to heat the water tank 1, which can not only speed up the oxygen precipitation speed in the water, but also improve the reaction speed of iron blood and oxygen. When the oxygen content in the water is reduced, the zebrafish cannot breathe oxygen in the water, resulting in a decrease in the oxygen content in the blood, thereby inducing blood circulation disorders in the zebrafish. This method does not require the use of chemical agents and is safe and easy to operate.

[0037] A sealing cover 5 is installed on the top of the filter frame 22, and a filter mesh frame 6 is installed on the bottom of the sealing cover 5. The iron filings 23 are located in the filter mesh frame 6. Through the setting of the sealing cover 5, it is convenient to seal the top of the filter frame 22 to ensure the sealing of the filter frame 22, and prevent the outside air from entering the circulation pipe 21 through the filter frame 22, resulting in the oxygen content in the water cannot be reduced, and then unable to induce zebrafish microcirculation disorders.

[0038] Two groups of guide plates 7 are installed in the filter frame 22. The guide plates 7 are arranged in a staggered manner. The outer side of the filter mesh frame 6 is attached to the inner wall of the filter frame 22 and the outer wall of the guide plates 7. The setting of the guide plates 7 facilitates the diversion of the aquaculture water flowing through the filter frame 22, prolongs the time for the aquaculture water to flow through the filter frame 22, and leaves enough time for the iron filings 23 to react with the oxygen in the water and consume the oxygen.

[0039] Both ends of the water tank 1 are connected with a right-angle elbow 8, and the end of the circulation pipe 21 away from the filter frame 22 is inserted into the inside of the right-angle elbow 8. The outer side of the circulation pipe 21 is provided with a first tightening threaded sleeve 9, and the first tightening threaded sleeve 9 is threadedly connected to the end of the right-angle elbow 8 away from the water tank 1. The circulation pipe 21 is inserted into the right-angle elbow 8, and then the circulation pipe 21 is limited and tightened by the first tightening threaded sleeve 9 to ensure that the circulation pipe 21 is connected to the water tank 1 through the right-angle elbow 8, forming a connected water circulation system, so that the aquaculture water in the water tank 1 continuously flows through the filter frame 22 to reduce the oxygen content in the water.

[0040] The top of the tank cover 31 is connected to a linear joint 10, and the end of the air guide tube 32 away from the vacuum pump 33 is inserted into the interior of the linear joint 10. The outer side of the air guide tube 32 is sleeved with a second tightening threaded sleeve 11, and the second tightening threaded sleeve 11 is threadedly connected to the end of the linear joint 10 away from the tank cover 31. Through the cooperation of the linear joint 10 and the second tightening threaded sleeve 11, the air guide tube 32 is conveniently connected to the tank cover 31 to ensure that the vacuum pump 33 is started, so that the air in the water tank 1 can be extracted to form a quasi-vacuum state.

[0041] The water circulation filtration component 2 also includes a water pump 24, which is installed on the circulation pipe 21. A control valve 12 is installed in the right-angle elbow 8. By installing the water pump 24 on the circulation pipe 21, the aquaculture water is driven to speed up the flow of the aquaculture water in the circulation pipe 21, so that the aquaculture water in the water tank 1 is continuously replaced. At the same time, the control valve 12 on the right-angle elbow 8 can control the on and off of the circulation system in the circulation pipe 21.

[0042] A plurality of mounting holes are provided on the outer side of the sealing cover 5, and mounting screws 13 are movably provided in the mounting holes. One end of the mounting screw 13 passes through the sealing cover 5 and is threadedly connected to the filter frame 22. The sealing cover 5 is installed on the top of the filter frame 22 by using the mounting screw 13 to ensure the sealing of the filter frame 22, and at the same time facilitate the replacement of the iron filings 23 in the filter mesh frame 6.

[0043] The inner cavity of the tank cover 31 is provided with a groove, and the groove is filled with a sealing ring 14. The sealing ring 14 passes through the groove and is squeezed and fitted with the outer wall of the water tank 1. By filling the sealing ring 14 in the groove and then clamping the tank cover 31 on the top of the water tank 1, the gap between the tank cover 31 and the water tank 1 is filled with the sealing ring 14 to ensure the sealing.

[0044] Filter plates 15 are installed on both sides of the inner cavity of the water tank 1. The filter plates 15 are located on the outer side of the end of the circulation pipe 21. By installing two groups of filter plates 15 in the water tank 1, zebrafish are prevented from entering the circulation pipe 21 and causing blockage of the circulation pipe 21.

[0045] A support base 16 is provided at the bottom of the temperature control device 4, and a slot 17 for engaging with the vacuum pump 33 and the temperature control device 4 is provided at the top of the support base 16. A placement rack 18 is fixed to the top of the support base 16 and is in contact with the bottom of the water pump 24 and the filter frame 22. The support base 16 is used to facilitate the limited support of the vacuum pump 33, the water tank 1, the water pump 24 and the filter frame 22 to ensure the stability of the device structure.

[0046] When used specifically, the zebrafish is placed in the aquaculture water in the water tank 1, and then the tank cover 31 is covered, and the water pump 24 is started. The aquaculture water is circulated by the water pump 24. When the aquaculture water passes through the filter frame 22 in the middle section of the circulation pipe 21, the oxygen in the water contacts and reacts with the iron filings 23 in the filter frame 6, consuming the oxygen in the water. After continuous water circulation, the oxygen in the aquaculture water in the water tank 1 is gradually reduced. Then the vacuum pump 33 is started, and the vacuum pump 33 extracts the air in the upper layer of the inner cavity of the water tank 1 through the air guide pipe 32, so that a vacuum state is formed above the aquaculture water, which promotes the precipitation of the gas dissolved in the water, and then further reduces the oxygen content in the water. At the same time, the temperature control device 4 can heat the aquaculture water in the water tank 1, which not only accelerates the consumption of oxygen, but also accelerates the precipitation speed of oxygen. Since the zebrafish is in the aquaculture water, the reduction of the oxygen content in the aquaculture water directly leads to the reduction of the oxygen content in the blood circulation of the zebrafish, inducing microcirculation disorders.

[0047] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for inducing blood microcirculation disorder in zebrafish, characterized in that: include: A tank for holding zebrafish (1); A water circulation filter assembly (2) arranged outside the water tank (1); The water circulation filter assembly (2) comprises a circulation pipe (21) installed on both sides of the water tank (1), a filter frame (22) is fixedly connected to the middle section of the circulation pipe (21), and the inner cavity of the filter frame (22) is filled with iron filings (23); A vacuum extraction component (3) arranged outside the water tank (1); The vacuum extraction assembly (3) comprises a tank cover (31) mounted on the top of the water tank (1); an air guide pipe (32) is mounted on the top of the tank cover (31); and an end of the air guide pipe (32) away from the tank cover (31) is connected to a vacuum pump (33); It also includes a temperature control device (4) located at the bottom of the water tank (1).

2. The device for inducing blood microcirculation disorder in zebrafish according to claim 1, characterized in that: A sealing cover (5) is installed on the top of the filter frame (22), a filter screen frame (6) is installed on the bottom of the sealing cover (5), and the iron filings (23) are located inside the filter screen frame (6).

3. The device for inducing blood microcirculation disorder in zebrafish according to claim 2, characterized in that: Two groups of guide plates (7) are installed in the filter frame (22), and the guide plates (7) are arranged in a staggered manner. The outer side of the filter screen frame (6) is attached to the inner wall of the filter frame (22) and the outer wall of the guide plates (7).

4. The device for inducing blood microcirculation disorder in zebrafish according to claim 1, characterized in that: Both ends of the water tank (1) are connected to right-angle elbows (8); one end of the circulation pipe (21) away from the filter frame (22) is inserted into the inside of the right-angle elbow (8); the outer side of the circulation pipe (21) is sleeved with a first tightening threaded sleeve (9); the first tightening threaded sleeve (9) is threadedly connected to the end of the right-angle elbow (8) away from the water tank (1).

5. The device for inducing blood microcirculation disorder in zebrafish according to claim 1, characterized in that: The top of the slot cover (31) is connected to a linear joint (10); one end of the air guide tube (32) away from the vacuum pump (33) is inserted into the interior of the linear joint (10); a second tightening threaded sleeve (11) is sleeved on the outer side of the air guide tube (32); the second tightening threaded sleeve (11) is threadedly connected to one end of the linear joint (10) away from the slot cover (31).

6. The device for inducing blood microcirculation disorder in zebrafish according to claim 4, characterized in that: The water circulation filter assembly (2) further comprises a water pump (24), wherein the water pump (24) is installed on the circulation pipeline (21), and a control valve (12) is installed in the right-angle elbow (8).

7. The device for inducing blood microcirculation disorder in zebrafish according to claim 2, characterized in that: A plurality of mounting holes are provided on the outer side of the sealing cover (5), and mounting screws (13) are movably arranged in the mounting holes. One end of the mounting screw (13) passes through the sealing cover (5) and is threadedly connected to the filter frame (22).

8. The device for inducing blood microcirculation disorder in zebrafish according to claim 1, characterized in that: The inner cavity of the tank cover (31) is provided with a groove, and the groove is filled with a sealing ring (14); the sealing ring (14) passes through the groove and is pressed and fitted with the outer wall of the water tank (1).

9. The device for inducing blood microcirculation disorder in zebrafish according to claim 1, characterized in that: Filter plates (15) are installed on both sides of the inner cavity of the water tank (1), and the filter plates (15) are located outside the end of the circulation pipeline (21).

10. The device for inducing blood microcirculation disorder in zebrafish according to claim 1, characterized in that: A support seat (16) is provided at the bottom of the temperature control device (4); a slot (17) for engaging with the vacuum pump (33) and the temperature control device (4) is provided at the top of the support seat (16); and a placement rack (18) is fixed to the top of the support seat (16) and is fitted to the bottom of the water pump (24) and the filter frame (22).

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