Parasite nucleic acid extraction device

Abstract

The utility model discloses a parasite nucleic acid extraction device and relates to the technical field of grinding extraction. Comprising an extracting tank, a grinding and extracting assembly used for crushing parasitic cells is arranged in the extracting tank, a grinding disc is fixedly installed on the inner wall of the extracting tank, a partition assembly is fixedly installed at the bottom of the inner side of the extracting tank, and a rotating part of the grinding and extracting assembly is attached to the surface of the grinding disc and the surface of the partition assembly. According to the parasite cell extraction device, parasite cells and an extracting solution are added into the extraction tank, the grinding extraction assembly is attached to the surface of the grinding disc and the surface of the partition assembly, then cell membranes of the parasite cells are broken in a mechanical mode, DNA or RNA is released, the parasite nucleic acid extracting solution is filtered through the filtering assembly, and the extraction efficiency is improved. Through the air pump, a pressure difference is generated on the upper side and the lower side of the filter material in the filter assembly through the C-shaped pipeline, and the filter efficiency of the parasite nucleic acid extracting solution is improved under the action of pressure.

Description

Technical Field

[0001] The utility model relates to the technical field of grinding and extraction, in particular to a parasite nucleic acid extraction device. Background Art

[0002] Parasite nucleic acid extraction is the process of extracting DNA or RNA from parasite samples for subsequent molecular biological analysis, PCR testing, sequencing, etc. Parasite cells are usually enclosed in cell membranes or walls, and the cell membranes need to be broken by mechanical or chemical methods to release DNA or RNA.

[0003] When the existing nucleic acid extraction and grinding device is used, it usually utilizes a grinding roller to perform unidirectional rotation grinding on the nucleic acid, which results in poor nucleic acid grinding efficiency and thus relatively low nucleic acid extraction efficiency. In addition, when performing nucleic acid extraction, the extract needs to be filtered, and the filtering efficiency is relatively low.

[0004] For this purpose, a parasite nucleic acid extraction device is proposed. Utility Model Content

[0005] The purpose of the utility model is to solve the problems raised in the above background technology, and provide a parasite nucleic acid extraction device.

[0006] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0007] A parasite nucleic acid extraction device comprises an extraction tank, wherein a grinding and extraction assembly for crushing parasite cells is arranged inside the extraction tank, a grinding disc is fixedly mounted on the inner wall of the extraction tank, a baffle assembly is fixedly mounted on the inner bottom of the extraction tank, a rotating portion of the grinding and extraction assembly is arranged in contact with the surfaces of the grinding disc and the baffle assembly, a filtering assembly is arranged on the bottom surface of the extraction tank, and a C-shaped pipe is arranged on one side wall of the filtering assembly, and an air pump is arranged on the surface of the C-shaped pipe.

[0008] Furthermore, the grinding and extraction component includes a motor, a motor is fixedly mounted on the top surface of the extraction tank, a rotating frame is fixedly mounted on the output end of the motor, a gear ring is fixedly mounted on the inner top of the extraction tank, a rotating shaft and a mixing cleaning scraper are rotatably plugged into the surface of the rotating frame, a grinding roller is sleeved on the surface of the rotating shaft, gears are fixedly mounted on the top of the rotating shaft and the mixing cleaning scraper, and the gears and the gear ring are meshingly mounted.

[0009] Furthermore, the filter assembly includes a discharge pipe, the bottom surface of the extraction tank is provided with a discharge pipe, and an electromagnetic control valve is provided in the discharge pipe, a filter box is fixedly installed at the bottom end of the discharge pipe, a plug-in rail is fixedly installed on the inner wall of the filter box, and a filter plate is plugged into the inside of the plug-in rail, and a C-shaped pipe is provided on the side wall of the filter box.

[0010] Furthermore, a plurality of through holes are provided on the surface of the baffle assembly, and the discharge pipe and the baffle assembly are arranged correspondingly.

[0011] Furthermore, the grinding roller is located between the grinding disc and the barrier assembly, and the surfaces of the grinding roller and the mixed cleaning scraper are respectively arranged to fit the surfaces of the grinding disc and the barrier assembly.

[0012] Furthermore, two ends of the C-shaped pipe are respectively located on the upper and lower sides of the filter plate.

[0013] The beneficial effects of the utility model are as follows:

[0014] By adding parasite cells and extract into the interior of the extraction tank, the grinding extraction component is fitted with the surface of the grinding disk and the baffle component, so that the cell membrane of the parasite cells is mechanically broken to release DNA or RNA, the parasite nucleic acid extract is filtered through the filtering component, and the C-shaped pipe is used by the air pump to generate a pressure difference on the upper and lower sides of the filter material inside the filtering component, thereby improving the filtration efficiency of the parasite nucleic acid extract under the action of pressure. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0016] Figure 2 It is a front view of the utility model;

[0017] Figure 3 It is a front cross-sectional view of the utility model;

[0018] Figure 4 This utility model Figure 3 A magnified view of part A;

[0019] Figure 5 This is a schematic diagram of the utility model baffle assembly

[0020] Figure numerals: 1. extraction tank; 2. grinding and extraction assembly; 201. motor; 202. rotating frame; 203. gear ring; 204. rotating shaft; 205. gear; 206. grinding roller; 207. mixing cleaning scraper; 3. grinding disc; 4. barrier assembly; 41. through hole; 5. filter assembly; 501. discharge pipe; 502. filter box; 503. plug-in rail; 504. filter plate; 6. C-shaped pipe; 7. air pump. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0022] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. In addition, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0024] In the description of the embodiments of the present invention, it should be noted that the terms "inside", "outside", "upper", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the utility model product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0025] like Figures 1 to 5As shown, a parasite nucleic acid extraction device comprises an extraction tank 1, wherein a grinding extraction component 2 for crushing parasite cells is arranged inside the extraction tank 1, a grinding disc 3 is fixedly installed on the inner wall of the extraction tank 1, a baffle component 4 is fixedly installed on the inner bottom of the extraction tank 1, a rotating part of the grinding extraction component 2 is arranged in contact with the surface of the grinding disc 3 and the baffle component 4, a filtering component 5 is arranged on the bottom surface of the extraction tank 1, and a C-shaped pipe 6 is arranged on one side wall of the filtering component 5, and an air pump 7 is arranged on the surface of the C-shaped pipe 6. More specifically, by adding parasite cells and extracting liquid into the inside of the extraction tank 1, the grinding extraction component 2 is attached to the surface of the grinding disc 3 and the baffle component 4, so that the cell membrane of the parasite cells is mechanically broken to release DNA or RNA, the parasite nucleic acid extract is filtered by the filtering component 5, and the C-shaped pipe 6 is used by the air pump 7 to generate a pressure difference on the upper and lower sides of the filter material inside the filtering component 5, thereby improving the filtering efficiency of the parasite nucleic acid extract under the action of pressure.

[0026] The grinding and extraction component 2 includes a motor 201, the top surface of the extraction tank 1 is fixedly mounted with the motor 201, the output end of the motor 201 is fixedly mounted with a rotating frame 202, the inner top of the extraction tank 1 is fixedly mounted with a gear ring 203, the surface of the rotating frame 202 is rotatably plugged with a rotating shaft 204 and a mixing cleaning scraper 207, the surface of the rotating shaft 204 is sleeved with a grinding roller 206, the top of the rotating shaft 204 and the mixing cleaning scraper 207 are fixedly mounted with a gear 205, and the gear 205 and the gear ring 203 are meshingly installed. More specifically, the motor 201 drives the rotating frame 202 to rotate, and the rotating frame 202 drives the rotating shaft 204 and the mixing cleaning scraper 207 to rotate, and the gear 205 and the gear ring 203 are engaged, so that the rotating shaft 204 and the mixing cleaning scraper 207 are rotated, and the rotating shaft 204 and the gear 205 rotate and rotate along the surface of the grinding disk 3 and the barrier component 4 to break the parasite cells, and the mixing cleaning scraper 207 rotates to clean the surface of the grinding disk 3 and the barrier component 4.

[0027] The filter assembly 5 includes a discharge pipe 501. The bottom surface of the extraction tank 1 is provided with the discharge pipe 501, and an electromagnetic control valve is provided in the discharge pipe 501. A filter box 502 is fixedly installed at the bottom end of the discharge pipe 501. A plug-in rail 503 is fixedly installed on the inner wall of the filter box 502, and a filter plate 504 is plugged into the plug-in rail 503. A C-shaped pipe 6 is provided on the side wall of the filter box 502. More specifically, by opening the solenoid valve in the discharge pipe 501, the discharge pipe 501 in the extraction tank 1 guides the parasite nucleic acid extract in the extraction tank 1 into the interior of the filter box 502, and the parasite nucleic acid extract is filtered through the filter plate 504. The filter plate 504 is plugged into the plug-in track 503 to facilitate the replacement of the filter plate 504. The air pump 7 allows the gas in the space above the filter plate 504 in the filter box 502 to be added to the space below the filter plate 504 in the filter box 502 through the C-shaped pipe 6, thereby generating a pressure difference and improving the filtration efficiency.

[0028] The surface of the barrier component 4 is provided with a plurality of through holes 41, and the discharge pipe 501 is arranged corresponding to the barrier component 4. More specifically, the plurality of through holes 41 on the surface of the barrier component 4 facilitates the discharge of the liquid in the extraction tank 1 through the discharge pipe 501.

[0029] The grinding roller 206 is located between the grinding disc 3 and the barrier assembly 4, and the surfaces of the grinding roller 206 and the mixed cleaning scraper 207 are respectively arranged to fit the surfaces of the grinding disc 3 and the barrier assembly 4. More specifically, the grinding roller 206 is located between the grinding disc 3 and the barrier assembly 4, and the grinding roller 206 and the mixed cleaning scraper 207 fit the surfaces of the grinding disc 3 and the barrier assembly 4, thereby facilitating the crushing of cells by the grinding roller 206, and cleaning the residues on the surfaces of the grinding disc 3 and the barrier assembly 4 by the mixed cleaning scraper 207.

[0030] The two ends of the C-shaped pipe 6 are respectively located at the upper and lower sides of the filter plate 504. More specifically, the two ends of the C-shaped pipe 6 are respectively located at the upper and lower sides of the filter plate 504 so that the air above the filter plate 504 can be introduced to the bottom of the filter plate 504.

[0031] In summary: by adding parasite cells and extract into the interior of the extraction tank 1, the grinding extraction component 2 is fitted with the surface of the grinding disk 3 and the baffle component 4, so that the cell membrane of the parasite cells is mechanically broken to release DNA or RNA, the parasite nucleic acid extract is filtered through the filtering component 5, and the C-shaped pipe 6 is used by the air pump 7 to generate a pressure difference on the upper and lower sides of the filter material inside the filtering component 5, thereby improving the filtration efficiency of the parasite nucleic acid extract under the action of pressure.

[0032] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and the specification only describe the principles of the utility model. The utility model may be subject to various changes and improvements without departing from the spirit and scope of the utility model. These changes and improvements fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A parasite nucleic acid extraction device, characterized in that: The invention comprises an extraction tank (1), wherein a grinding extraction component (2) for crushing parasite cells is arranged inside the extraction tank (1), a grinding disc (3) is fixedly mounted on the inner wall of the extraction tank (1), a baffle component (4) is fixedly mounted on the inner bottom of the extraction tank (1), a rotating part of the grinding extraction component (2) is arranged in contact with the surfaces of the grinding disc (3) and the baffle component (4), a filtering component (5) is arranged on the bottom surface of the extraction tank (1), and a C-shaped pipe (6) is arranged on one side wall of the filtering component (5), and an air pump (7) is arranged on the surface of the C-shaped pipe (6).

2. A parasite nucleic acid extraction device according to claim 1, characterized in that: The grinding and extraction component (2) comprises a motor (201), the motor (201) is fixedly mounted on the top surface of the extraction tank (1), a rotating frame (202) is fixedly mounted on the output end of the motor (201), a gear ring (203) is fixedly mounted on the inner top of the extraction tank (1), a rotating shaft (204) and a mixing cleaning scraper (207) are rotatably plugged into the surface of the rotating frame (202), a grinding roller (206) is sleeved on the surface of the rotating shaft (204), gears (205) are fixedly mounted on the top ends of the rotating shaft (204) and the mixing cleaning scraper (207), and the gears (205) and the gear ring (203) are meshingly mounted.

3. A parasite nucleic acid extraction device according to claim 1, characterized in that: The filter assembly (5) comprises a discharge pipe (501), the bottom surface of the extraction tank (1) is provided with a discharge pipe (501), and an electromagnetic control valve is provided inside the discharge pipe (501), a filter box (502) is fixedly installed at the bottom end of the discharge pipe (501), a plug-in rail (503) is fixedly installed on the inner wall of the filter box (502), and a filter plate (504) is plugged into the plug-in rail (503), and a C-shaped pipe (6) is provided on the side wall of the filter box (502).

4. A parasite nucleic acid extraction device according to claim 3, characterized in that: A plurality of through holes (41) are provided on the surface of the baffle assembly (4), and the discharge pipe (501) and the baffle assembly (4) are arranged correspondingly.

5. A parasite nucleic acid extraction device according to claim 2, characterized in that: The grinding roller (206) is located between the grinding disc (3) and the barrier assembly (4), and the surfaces of the grinding roller (206) and the mixing cleaning scraper (207) are respectively arranged to fit the surfaces of the grinding disc (3) and the barrier assembly (4).

6. A parasite nucleic acid extraction device according to claim 3, characterized in that: The two ends of the C-shaped pipe (6) are respectively located on the upper and lower sides of the filter plate (504).

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