Protein detection and purification equipment

Abstract

The utility model discloses protein detection and purification equipment, which belongs to the technical field of protein purification and is characterized in that an adding tank is arranged to be matched with a sealing disc, the sealing disc rotates to drive a conveying groove to adjust the position, and when the conveying groove moves to the connecting position of a connecting channel and a raw material groove, the raw material groove is connected with the feeding tank. Compared with the prior art, the eluent purification device has the advantages that the eluents with different components can be automatically added, the purification efficiency is improved, the situation of misoperation is reduced, the synchronous adjusting mechanism is arranged, a driven ring rotates to drive a gear ring to rotate, the gear ring enables a fluted disc connected in a meshed mode to rotate, and the eluent in the raw material tank is conveyed. The rotation of the fluted disc realizes the movement of the support frame and the glass tube, so that the glass tube contains different molecular liquids flowing out of the separation tube, and compared with the prior art, when the eluent is replaced, the collection vessel is automatically replaced, and the operation convenience of the purification equipment is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of protein purification, and more specifically to a protein detection and purification device. Background Art

[0002] When performing protein detection operations, in order to ensure the accuracy of the test results and avoid adverse effects of other impurities on the test results, purification equipment is required to purify the protein. Gel filtration chromatography is one of the methods for fine protein separation. It uses porous gel beads as a matrix to separate proteins according to molecular size. Generally, large molecules flow out first and small molecules flow out later.

[0003] Based on the above, the inventors found that when the existing purification equipment is used, it is necessary to gradually elute the specifically bound protein by changing the composition of the elution buffer, so it is necessary to add a variety of different eluents in sequence. Since the addition of the eluent is mainly done manually by workers, it is easy to have the problem of disordered addition order, which affects the protein purification effect. Therefore, in view of this, the existing structure is studied and improved to provide a protein detection and purification equipment, in order to achieve a more practical purpose. Utility Model Content

[0004] 1. Technical issues to be solved

[0005] In view of the problems existing in the prior art, the purpose of the utility model is to provide a protein detection and purification device. In this scheme, an addition tank is provided to cooperate with a sealing disk. The rotation of the sealing disk drives the conveying trough to adjust the position. When the conveying trough moves to the connection between the connecting channel and the raw material tank, the eluent inside the raw material tank is conveyed. Compared with the prior art, the addition of eluents with different components can be automatically performed, which improves the purification efficiency and reduces the possibility of operational errors. By providing a synchronous adjustment mechanism, the rotation of the driven ring drives the rotation of the gear ring, and the gear ring rotates the meshing gear disk. The rotation of the gear disk realizes the movement of the support frame and the glass tube, so that the glass tube can hold different molecular liquids flowing out of the separation tube. Compared with the prior art, when the eluent is replaced, the collection vessel is automatically replaced, which improves the operational convenience of the purification equipment.

[0006] 2. Technical solution

[0007] To solve the above problems, the utility model adopts the following technical solutions.

[0008] A protein detection and purification device comprises a base, wherein a synchronous adjustment mechanism is centrally arranged on the top surface of the base, and a separation tube and an addition tank are respectively fixedly connected to the upper side of the base on both sides, a porous plate is fixedly connected to the lower end of the separation tube, a driving motor is fixedly connected to the upper side of the output end of the driving motor, a driving ring is fixedly connected to the upper side, a driven ring is symmetrically arranged on one side of the driving ring, and the outer sides of the driving ring and the driven ring are sleeved with the same transmission belt, a group of raw material troughs are opened at the upper side of the addition tank, and a connecting channel is opened at the lower side of the addition tank, a liquid infusion tube is fixedly connected to the bottom end of the connecting channel, a sealing disk is fixedly connected to the lower end of the output end of the driving motor, and a conveying groove is opened on one side of the sealing disk.

[0009] Furthermore, the synchronous adjustment mechanism includes a toothed disc, one side of which is meshedly connected with a toothed ring, and the bottom surfaces of the toothed disc and the toothed ring are fixedly connected with movable rings, and the top surface of the toothed disc is fixedly connected with a group of support frames, and a glass tube is clamped inside the support frame.

[0010] Furthermore, the separation tube is filled with gel particles, different raw material tanks are filled with different eluents, and one end of the infusion tube is fixedly connected to the upper end of the separation tube.

[0011] Furthermore, the sealing disk is located inside the adding tank, and the sealing disk is movably connected to the adding tank.

[0012] Furthermore, the upper end of the connecting channel is connected to a plurality of raw material troughs, and the conveying trough is adapted to the connection between the connecting channel and the raw material trough.

[0013] Furthermore, the gear ring is fixedly connected to the driven ring, the movable ring is located inside the base, and the movable ring is movably connected to the base.

[0014] Furthermore, the glass tube is adapted to the raw material tank, and the inner glass tube among the plurality of glass tubes is located directly below the separation tube.

[0015] 3. Beneficial effects

[0016] Compared with the prior art, the advantages of the present invention are:

[0017] (1) This solution provides an addition tank in conjunction with a sealing disk. The rotation of the sealing disk drives the conveying trough to adjust its position. When the conveying trough moves to the connection between the connecting channel and the raw material trough, the eluent in the raw material trough is conveyed. Compared with the prior art, the addition of eluents of different components can be performed automatically, thereby improving the purification efficiency and reducing the possibility of operational errors.

[0018] (2) By setting up a synchronous adjustment mechanism, the rotation of the driven ring drives the rotation of the gear ring, and the gear ring causes the meshing gear disk to rotate. The rotation of the gear disk realizes the movement of the support frame and the glass tube, so that the glass tube can hold different molecular liquids flowing out of the separation tube. Compared with the existing technology, when the eluent is replaced, the collection container is automatically replaced, thereby improving the operating convenience of the purification equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0020] Figure 2 It is a cross-sectional view of the overall structure of the utility model;

[0021] Figure 3 This is a disassembled diagram of the adding tank and the sealing disk of the utility model;

[0022] Figure 4 It is a structural schematic diagram of the synchronous adjustment mechanism of the utility model.

[0023] Explanation of the numbers in the figure: 1. Base; 2. Synchronous adjustment mechanism; 3. Separation tube; 4. Adding tank; 5. Perforated plate; 6. Driving motor; 7. Active ring; 8. Driven ring; 9. Transmission belt; 10. Raw material tank; 11. Connecting channel; 12. Infusion tube; 13. Sealing disk; 14. Conveying trough; 15. Toothed disk; 16. Toothed ring; 17. Movable ring; 18. Support frame; 19. Glass tube. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model; it is obvious that the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments of the utility model without making creative work are within the scope of protection of the utility model.

[0025] Example:

[0026] See also Figure 1-4A protein detection and purification device comprises a base 1, a synchronous adjustment mechanism 2 is arranged in the center of the top surface of the base 1, and a separation tube 3 and an addition tank 4 are fixedly connected to the upper and lower sides of the base 1, a porous plate 5 is fixedly connected to the lower end of the separation tube 3, a driving motor 6 is fixedly connected to the upper and lower sides of the addition tank 4, an active ring 7 is fixedly connected to the output end of the driving motor 6, a driven ring 8 is symmetrically arranged on one side of the active ring 7, and the outer sides of the active ring 7 and the driven ring 8 are sleeved with the same transmission belt 9, a group of raw material troughs 10 are opened in the upper part of the addition tank 4, and a connecting channel 11 is opened in the lower part of the addition tank 4, a liquid infusion tube 12 is fixedly connected to the bottom end of the connecting channel 11, a sealing disk 13 is fixedly connected to the output end of the driving motor 6 near the bottom, a conveying groove 14 is opened on one side of the sealing disk 13, the driving motor 6 is started, the active ring 7 and the sealing disk 13 are driven to rotate synchronously, and the active ring 7 cooperates with the transmission belt 9 to rotate the driven ring 8.

[0027] See also Figure 4 The synchronous adjustment mechanism 2 includes a toothed disc 15, one side of which is meshedly connected with a toothed ring 16, and the bottom surfaces of the toothed disc 15 and the toothed ring 16 are fixedly connected with a movable ring 17, and the top surface of the toothed disc 15 is fixedly connected with a group of support frames 18, and the interior of the support frames 18 is clamped with a glass tube 19. In order to automatically replace the collecting vessel when the eluent is replaced and improve the operating convenience of the purification equipment, a synchronous adjustment mechanism 2 is provided.

[0028] See also Figure 2 The separation tube 3 is filled with gel particles, and different raw material tanks 10 are filled with different eluents. One end of the infusion tube 12 is fixedly connected to the upper end of the separation tube 3, and the eluent is transported to the separation tube 3 by the infusion tube 12. The eluent drives the protein to pass through the gel particles inside the separation tube 3.

[0029] See also Figure 3 The sealing disk 13 is located inside the adding tank 4, and the sealing disk 13 is movably connected to the adding tank 4. The rotation of the sealing disk 13 drives the conveying groove 14 to adjust the position. When the conveying groove 14 moves to the connection between the connecting channel 11 and the raw material tank 10, the eluent inside the corresponding raw material tank 10 passes through the conveying groove 14 and enters the inside of the connecting channel 11.

[0030] See also Figure 3 The upper end of the connecting channel 11 is connected to a plurality of raw material tanks 10 , the conveying tank 14 is adapted to the connection between the connecting channel 11 and the raw material tank 10 , and the connecting channel 11 conveys the eluent to the infusion tube 12 .

[0031] See also Figure 4The gear ring 16 is fixedly connected to the driven ring 8, the movable ring 17 is located inside the base 1, and the movable ring 17 is movably connected to the base 1. The rotation of the driven ring 8 drives the gear ring 16 to rotate, and the gear ring 16 causes the meshing toothed disc 15 to rotate, and the movable ring 17 ensures its rotation stability.

[0032] See also Figure 4 The glass tube 19 is matched with the raw material tank 10, and the inner glass tube 19 among the plurality of glass tubes 19 is located directly below the separation tube 3. The rotation of the toothed disk 15 realizes the movement of the support frame 18 and the glass tube 19, so that the glass tube 19 can hold different molecular liquids flowing out of the separation tube 3.

[0033] When in use: the base 1, the synchronous adjustment mechanism 2, the separation tube 3 and the addition tank 4 constitute the main part of the entire purification equipment. First, put the protein raw material into the separation tube 3, start the driving motor 6, drive the active ring 7 and the sealing disk 13 to rotate synchronously, the active ring 7 cooperates with the transmission belt 9 to rotate the driven ring 8, the rotation of the driven ring 8 drives the gear ring 16 to rotate, the gear ring 16 rotates the meshing toothed disk 15, and the movable ring 17 ensures its rotation stability. The rotation of the toothed disk 15 realizes the movement of the support frame 18 and the glass tube 19, so that the corresponding glass tube 19 moves to the bottom of the separation tube 3, and at the same time the rotation of the sealing disk 13 The conveying groove 14 is driven to adjust its position. When the conveying groove 14 moves to the connection point between the connecting channel 11 and the raw material groove 10, the eluent in the corresponding raw material groove 10 passes through the conveying groove 14 and enters the connecting channel 11. Finally, the eluent is transported to the separation tube 3 through the connecting channel 11 in cooperation with the infusion tube 12. The eluent drives the macromolecular part of the protein to pass through the gel particles in the separation tube 3 and is collected by the corresponding glass tube 19. Then, the driving motor 6 rotates again to drive the release of new eluent and adjust the position of the new glass tube 19 to complete the protein purification and separation work of the gel filtration chromatography method.

[0034] Finally, it should be noted that in the description of the present invention, it should be noted that the terms "vertical", "up", "down", "horizontal", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limitations on the present invention.

[0035] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0036] The above is only a preferred specific implementation of the utility model; however, the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solution and improved ideas of the utility model within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model.

Claims

1. A protein detection and purification device, comprising a base (1), a synchronous adjustment mechanism (2) being arranged in the center of the top surface of the base (1), and a separation tube (3) and an addition tank (4) being fixedly connected to the upper and lower sides of the base (1), respectively, a porous plate (5) being fixedly connected to the lower end of the separation tube (3), a driving motor (6) being fixedly connected to the upper side of the output end of the driving motor (6), a driving ring (7) being fixedly connected to the upper side, a driven ring (8) being symmetrically arranged on one side of the driving ring (7), and a same transmission belt (9) being sleeved on the outer sides of the driving ring (7) and the driven ring (8), a group of raw material troughs (10) being arranged in the upper side of the addition tank (4), and a connecting channel (11) being arranged in the lower side of the addition tank (4), a liquid infusion tube (12) being fixedly connected to the lower end of the connecting channel (11), a sealing disk (13) being fixedly connected to the lower end of the output end of the driving motor (6), and a conveying groove (14) being arranged on one side of the sealing disk (13).

2. A protein detection and purification device according to claim 1, characterized in that: The synchronous adjustment mechanism (2) comprises a toothed disc (15), one side of the toothed disc (15) is meshingly connected with a toothed ring (16), and the bottom surfaces of the toothed disc (15) and the toothed ring (16) are fixedly connected with a movable ring (17), and the top surface of the toothed disc (15) is fixedly connected with a group of support frames (18), and the interior of the support frames (18) is clamped with a glass tube (19).

3. A protein detection and purification device according to claim 1, characterized in that: The separation tube (3) is filled with gel particles, the different raw material tanks (10) are filled with different eluents, and one end of the infusion tube (12) is fixedly connected to the upper end of the separation tube (3).

4. A protein detection and purification device according to claim 1, characterized in that: The sealing disk (13) is located inside the adding tank (4), and the sealing disk (13) is movably connected to the adding tank (4).

5. A protein detection and purification device according to claim 1, characterized in that: The upper end of the connecting channel (11) is connected to a plurality of raw material troughs (10), and the conveying trough (14) is adapted to the connection between the connecting channel (11) and the raw material trough (10).

6. A protein detection and purification device according to claim 2, characterized in that: The gear ring (16) is fixedly connected to the driven ring (8), the movable ring (17) is located inside the base (1), and the movable ring (17) is movably connected to the base (1).

7. A protein detection and purification device according to claim 2, characterized in that: The glass tube (19) is matched with the raw material tank (10), and the inner glass tube (19) among the plurality of glass tubes (19) is located directly below the separation tube (3).

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