Rapid early diagnosis detection card for porcine epidemic diarrhea virus

Abstract

The utility model relates to the technical field of detection cards, in particular to a porcine epidemic diarrhea virus rapid early diagnosis detection card which comprises two outer side plates and further comprises a bottom plate, the bottom plate is arranged on the lower surface of each outer side plate, a top plate is arranged on the upper surface of each outer side plate, a square hole is formed in the upper surface of the top plate, and the square hole is communicated with the bottom plate. The outer side plates are arranged on the upper surface of the top plate, the protection assembly is arranged on the upper surface of the top plate, the feeding assembly is arranged on the upper surface of the top plate, and the detection assemblies are arranged on the inner surfaces of the outer side plates. A virus sample easily flows out or splashes in the detection process, the protective layer can well absorb the virus sample, the safety is greatly improved, an operator needs to operate the detection card by hand, the metal round block has a strong antibacterial effect, and the harm of the virus to a human body is further prevented.

Description

Technical Field

[0001] The utility model relates to the technical field of detection cards, in particular to a rapid early diagnosis detection card for porcine epidemic diarrhea virus. Background Art

[0002] With the development of animal husbandry, the prevention and control of animal diseases has become an important part of ensuring the healthy development of animal husbandry. As a fast and convenient detection tool, veterinary virus detection cards play a key role in animal disease prevention and control. However, the veterinary virus detection cards currently on the market still have deficiencies in terms of safety and may pose a potential threat to the environment and operators.

[0003] After searching, the utility model patent with the Chinese publication number CN206725585U discloses a rapid early diagnosis test card for porcine epidemic diarrhea virus, which includes a support layer, a protective film, and a sample pad, a conjugation pad, a nitrocellulose layer and a water-absorbing pad are arranged in sequence above the support layer; colloidal gold labeled with recombinant N protein purified by Ni-NTA affinity chromatography and gel filtration chromatography is used as a detection probe on the conjugation pad; nitrocellulose membranes coated with Staphylococcus aureus protein A and His monoclonal antibody are used as detection lines and quality control lines respectively, and the assembly of the test card is completed together. The utility model is simple and fast to operate; it has a wide range of applications and is easy to promote and apply universally.

[0004] Although the above technical solution solves the problem of easy operation, in the above technical solution, the operator is required to put the test supernatant into the test port, which is easy to spill during the placement process and cause harm to the human body. In order to avoid harm to the human body caused by operational errors, a rapid early diagnosis test card for porcine epidemic diarrhea virus is needed. Utility Model Content

[0005] 1. Technical issues to be resolved

[0006] In view of the deficiencies of the prior art, the utility model provides a rapid early diagnosis test card for porcine epidemic diarrhea virus to solve the problem that the prior art, as mentioned in the background technology, poses a potential threat to the environment and operators.

[0007] (II) Technical solution

[0008] To achieve the above purpose, the utility model provides the following technical solution: comprising two outer side plates, and also comprising:

[0009] A bottom plate, the bottom plate being arranged on the lower surface of each of the outer plates;

[0010] A top plate, the top plate being arranged on the upper surface of each of the outer plates;

[0011] A square hole, wherein the square hole is provided on the upper surface of the top plate;

[0012] A protection component, wherein the protection component is arranged on the upper surface of the top plate;

[0013] A loading assembly, the loading assembly being arranged on the upper surface of the top plate;

[0014] A detection component is arranged on the inner surface of each of the outer plates.

[0015] Furthermore, the protection component includes:

[0016] A protective edge, the protective edge being arranged on the upper surface of the top plate;

[0017] A protective layer, the protective layer being arranged on the top plate;

[0018] A protection groove, wherein the protection groove is formed on the protection layer;

[0019] A side protection plate, the side protection plate being arranged on the outer side plate;

[0020] A metal round block is arranged on the side protection plate.

[0021] Furthermore, the feeding assembly includes:

[0022] A straight groove plate, wherein the straight groove plate is arranged on the top plate,

[0023] A straight groove convex ring is arranged on the inner surface of the straight groove plate.

[0024] As a further solution of the present application, the detection component includes:

[0025] A PVC board, wherein the PVC board is arranged on the upper surface of the bottom plate;

[0026] A sample pad, the sample pad being arranged on the upper surface of the base plate;

[0027] An antigen pad, the antigen pad being arranged on the lower surface of the sample pad;

[0028] NC film, the NC film is arranged on the upper surface of the PVC board;

[0029] A water-absorbing pad is disposed on the upper surface of the NC membrane.

[0030] As a further solution of the present application, the NC membrane is made of nitrocellulose membrane.

[0031] Based on the above scheme, the antigen pad is used to detect antigens.

[0032] (III) Beneficial effects

[0033] Compared with the prior art, the utility model provides a rapid early diagnosis test card for porcine epidemic diarrhea virus, which has the following beneficial effects:

[0034] 1. In the prior art, it is easy to splash when adding virus samples for testing. In the utility model, when the operator uses the diagnostic test card to detect the virus, the virus sample needs to be placed on the sample pad, during which the virus sample is easy to flow out or splash. The protective component can absorb the virus sample very well, greatly increasing safety.

[0035] 2. In the prior art, the outer side of the overall detection card is made of plastic, which cannot effectively isolate the virus. In the utility model, the operator needs to operate the detection card by hand, and the metal round block inside the protective component has a strong antibacterial effect, further preventing the virus from harming the human body. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a schematic diagram of the structure in the side view of this application;

[0037] Figure 2 It is a schematic diagram of the structure of the detection component in this application;

[0038] Figure 3 This is a schematic diagram of the structure of the feeding assembly in this application.

[0039] In the figure: 1. outer plate; 2. bottom plate; 3. top plate; 4. square hole; 5. protection component; 501. protection edge; 502. protection layer; 503. protection groove; 504. side protection plate; 505. metal round block; 6. loading component; 601. straight groove plate; 602. straight groove convex ring; 7. detection component; 701. PVC plate; 702. sample pad; 703. antigen pad; 704. NC membrane; 705. absorbent pad. DETAILED DESCRIPTION

[0040] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0041] For examples, see Figures 1 to 3, including two outer plates 1, and also including: a bottom plate 2, a top plate 3, a square hole 4, a protection component 5, a feeding component 6, and a detection component 7. The outer plate 1 plays a role in protecting both sides and also plays a role in supporting the protection component 5. The bottom plate 2 is fixedly connected to the lower surface of each outer plate 1. The function of the bottom plate 2 is to support the entire detection card, protect the safety of the detection card, and improve the accuracy of the detection. The top plate 3 is detachably connected to the upper surface of each outer plate 1. The function of the top plate 3 is to support the entire feeding component 6 and ensure the accuracy of the detection. The square hole 4 is opened on the upper surface of the top plate 3. The square hole 4 is used to facilitate observation of the test results, the protection component 5 is arranged on the upper surface of the top plate 3, the function of the protection component 5 is that when loading, the virus sample may be spilled outward, and the protection component 5 plays a role in inactivating the virus sample, the loading component 6 is arranged on the upper surface of the top plate 3, the function of the loading component 6 is to facilitate adding samples to the detection component 7, the detection component 7 is arranged on the inner surface of each outer plate 1, and the function of the detection component 7 is that the detection card adopts the immune reaction principle of specific antibodies and PEDV antigens, and realizes rapid detection of PEDV through the color development reaction on the detection card strip.

[0042] The protection component 5 includes: a protection edge 501, a protection layer 502, a protection groove 503, a side protection plate 504, and a metal round block 505. The protection edge 501 is fixedly connected to the upper surface of the top plate 3. The function of the protection edge 501 is to prevent the virus sample from flowing out and affecting people's health. The protection layer 502 is fixedly connected to the top plate 3. The protection layer 502 is composed of titanium dioxide, calcium phosphate, etc., which inactivate viruses and bacteria through a specific mechanism. The protection groove 503 is opened on the protection layer 502. The function of the protection groove 503 is to absorb the virus sample as the first step when it flows out to prevent the virus sample from spreading. The side protection plate 504 is fixedly connected to the outer plate 1. The function of the side protection plate 504 is to fix and support the metal round block 505. The metal round block 505 is fixedly connected to the side protection plate 504. The metal round block 505 is heavy metal ions such as copper. They have a strong antibacterial effect and can prevent virus transmission by adding other materials or applying to surface coatings.

[0043] The feeding assembly 6 includes: a straight groove plate 601 and a straight groove convex ring 602. The straight groove plate 601 is fixedly connected to the top plate 3. The function of the straight groove plate 601 is to facilitate the addition of virus samples and prevent the spread of virus samples. The straight groove convex ring 602 is fixedly connected to the inner surface of the straight groove plate 601. The function of the straight groove convex ring 602 is to prevent leakage when too much virus sample is added.

[0044] The detection component 7 includes: a PVC plate 701, a sample pad 702, an antigen pad 703, an NC membrane 704, and a water-absorbing pad 705. The PVC plate 701 is fixedly connected to the upper surface of the bottom plate 2. The PVC plate 701 has excellent chemical stability. The PVC plate 701 plays a role in supporting the detection component 7. The sample pad 702 is fixedly connected to the upper surface of the bottom plate 2. The function of the sample pad 702 is to facilitate the chromatography of the virus sample and prevent the virus sample from spreading. The antigen pad 703 is fixedly connected to the lower surface of the sample pad 702. The antigen pad 703 is used for tracking The reaction between antibodies and antigens, when the sample contains specific antigens, these antigens will combine with the colloidal gold labeled antibodies on the antigen pad 703 to form a complex, and then produce a specific color reaction on the test card, thereby realizing the detection of the antigen. The NC membrane 704 is fixedly connected to the upper surface of the PVC plate 701. The material of the NC membrane 704 is nitrocellulose membrane. The absorbent pad 705 is fixedly connected to the upper surface of the NC membrane 704. The function of the absorbent pad 705 is to absorb the virus sample after the virus sample is detected to prevent the virus sample from spreading and causing harm to the environment or human body.

[0045] In summary, when using this porcine epidemic diarrhea virus rapid early diagnosis test card, the operator first pinches the outer plate 1 with his hand, the metal round block 505 on the outer plate 1 can effectively isolate the virus, and the virus sample is placed in the straight groove plate 601. The straight groove convex ring 602 in the straight groove plate 601 can effectively prevent the virus sample from splashing. When the virus sample accidentally splashes out, the protective groove 503 first absorbs the splashed virus sample, and the protective layer 502 absorbs the excess virus sample. The detection effect is observed in the square hole 4. If the quality control line and the test line appear purple-red at the same time, it is positive, and if only one purple-red line appears in the control line area, it is negative. If neither of them appears red or only the test area appears color, it is invalid.

[0046] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A porcine epidemic diarrhea virus rapid early diagnosis test card, comprising two outer plates (1), characterized in that: Also includes: A bottom plate (2), the bottom plate (2) being arranged on the lower surface of each of the outer plates (1); A top plate (3), the top plate (3) being arranged on the upper surface of each of the outer plates (1); A square hole (4), wherein the square hole (4) is provided on the upper surface of the top plate (3); A protection component (5), wherein the protection component (5) is arranged on the upper surface of the top plate (3); A loading assembly (6), wherein the loading assembly (6) is arranged on the upper surface of the top plate (3); A detection component (7), wherein the detection component (7) is arranged on the inner surface of each of the outer plates (1).

2. A porcine epidemic diarrhea virus rapid early diagnosis test card according to claim 1, characterized in that: The protection component (5) comprises: A protective edge (501), the protective edge (501) being arranged on the upper surface of the top plate (3); A protective layer (502), the protective layer (502) being arranged on the top plate (3); A protection groove (503), wherein the protection groove (503) is formed on the protection layer (502); A side protection plate (504), wherein the side protection plate (504) is arranged on the outer side plate (1); A metal round block (505), wherein the metal round block (505) is arranged on the side protection plate (504).

3. A porcine epidemic diarrhea virus rapid early diagnosis test card according to claim 2, characterized in that: The feeding assembly (6) comprises: a straight groove plate (601), wherein the straight groove plate (601) is arranged on the top plate (3), A straight groove convex ring (602), wherein the straight groove convex ring (602) is arranged on the inner surface of the straight groove plate (601).

4. A porcine epidemic diarrhea virus rapid early diagnosis test card according to claim 3, characterized in that: The detection component (7) comprises: A PVC plate (701), the PVC plate (701) being arranged on the upper surface of the bottom plate (2); A sample pad (702), the sample pad (702) being arranged on the upper surface of the base plate (2); An antigen pad (703), wherein the antigen pad (703) is disposed on the lower surface of the sample pad (702); An NC film (704), wherein the NC film (704) is disposed on the upper surface of the PVC plate (701); A water-absorbing pad (705), wherein the water-absorbing pad (705) is arranged on the upper surface of the NC membrane (704).

5. A porcine epidemic diarrhea virus rapid early diagnosis test card according to claim 4, characterized in that: The NC membrane (704) is made of nitrocellulose membrane.

6. A porcine epidemic diarrhea virus rapid early diagnosis test card according to claim 4, characterized in that: The antigen pad (703) is used to detect antigens.

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