Microorganism culture device capable of simulating and controlling multi-dimensional environmental factors

Abstract

The utility model relates to the technical field of microorganism culture devices, and provides a microorganism culture device capable of simulating and controlling multi-dimensional environmental factors. The device comprises a box body, a box cover, a water quality online monitoring device, a nutrient adding device, a temperature control device and a detachable light barrier. A plurality of environmental factors capable of influencing the growth of microorganisms, such as water temperature, wind speed, nutrients and illumination intensity, can be simulated in the box body. The water quality on-line monitoring device can monitor the concentration of different substances in the box body in real time. The nutrient adding device can automatically add a nutrient solution into the box body, so that the simulation condition is stable. The device is suitable for simulating various surface water environments, and has the advantages of being sensitive in reaction, good in stability, convenient to sample, safe to use and convenient to adjust.

Description

Technical Field

[0001] The present application belongs to the technical field of surface water simulation experiments, and specifically relates to a microbial cultivation device capable of simulating and controlling multi-dimensional environmental factors. Background Art

[0002] Microorganisms in water environments can cause many problems, such as causing disease, secreting toxic substances, and producing odors. How to control the growth of microorganisms is an important issue to ensure the safety of drinking water microorganisms. Researchers at home and abroad have designed a variety of environmental water sample microbial culture devices, such as AR reactors, CDC reactors, etc. Some scholars directly use conical flasks and constant temperature incubators as equipment for cultivating microorganisms. These culture devices mainly focus on individual factors that affect the growth of microorganisms, such as water flow rate and temperature, and do not consider other factors, which brings certain limitations to the study of environmental problems caused by microorganisms. In addition, the nutrients required by microorganisms are mostly added manually on a regular basis or in excess at one time, which is not convenient for long-term cultivation. Utility Model Content

[0003] The purpose of the embodiments of the present application is to provide a microbial cultivation device that can simulate and control multi-dimensional environmental factors, so as to solve the technical problems existing in the related art that the device can simulate few factors and is not convenient for long-term cultivation.

[0004] According to an embodiment of the present application, there is provided: a microbial cultivation device capable of simulating and controlling multi-dimensional environmental factors, comprising:

[0005] A light-proof box body, with no cover on the top, an agitator installed inside, a fan installed on the side wall, and a temperature probe jack, a heating rod jack and a water inlet pipe jack on the side wall;

[0006] A box cover, covering the top of the box body, the box cover is provided with an observation hole, and a fluorescent tube is installed inside;

[0007] The water quality online monitoring device comprises a water inlet pipe, a water quality monitor, a water outlet pipe and a waste liquid bucket, wherein one end of the water inlet pipe is connected to the water inlet pipe jack, and the other end is connected to the inlet of the water quality monitor, one end of the water outlet pipe is connected to the outlet of the water quality monitor, and the other end is connected to the waste liquid bucket;

[0008] A nutrient adding device, comprising a plurality of nutrient storage tanks, a metering pump, and a controller, each nutrient storage tank storing different nutrients, each nutrient storage tank being equipped with a metering pump, an input port of the metering pump being connected to the nutrient storage tank, an output port being connected to the box body, and all metering pumps being electrically connected to the controller;

[0009] The temperature control device comprises a digital temperature controller, a temperature probe, and a heating rod, wherein the temperature probe is mounted on the temperature probe jack, the heating rod is mounted on the heating rod jack, and the digital temperature controller is electrically connected to the temperature probe and the heating rod respectively;

[0010] The detachable light shielding plate comprises a frame and a light shielding sheet. The frame is fixed on the box body, and the light shielding sheet is detachably mounted on the frame.

[0011] Optionally, the agitator includes a motor and a stirring blade mounted on a rotating shaft of the motor, and the stirring blade is located on a base of the box body.

[0012] Optionally, the fan can adjust the wind speed.

[0013] Optionally, a faucet is also installed on the side wall of the box body.

[0014] Optionally, the faucet is located at half the height of the box body to facilitate water sample collection.

[0015] Optionally, the fluorescent tubes are multiple and installed in parallel on the inner side of the box cover.

[0016] Optionally, the water quality detector includes a total organic carbon detector, a total phosphorus detector and a total nitrogen detector.

[0017] Optionally, the light shielding sheet comprises a plurality of light shielding sheets, and the plurality of light shielding sheets are spliced ​​and installed on the frame.

[0018] The technical solution provided by the embodiments of the present application may have the following beneficial effects:

[0019] 1) It can simulate and effectively control the water temperature, water flow rate, wind environment and light environment in the device, and observe the effects of different factors on the growth of microorganisms; 2) It uses automated control to add nutrients, and activates or shuts down the nutrient addition device at the concentration level detected at regular intervals to ensure the stability of the nutrient concentration in the culture device; 3) It is suitable for simulating a variety of surface water environments, and has the advantages of sensitive response, good stability, convenient sampling, safe use and easy adjustment.

[0020] It should be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0022] Figure 1It is a schematic structural diagram of a microorganism cultivation device capable of simulating and controlling multi-dimensional environmental factors according to an exemplary embodiment.

[0023] Figure 2 is a perspective view of a box body according to an exemplary embodiment;

[0024] Figure 3 is a schematic diagram of a detachable light shielding plate according to an exemplary embodiment;

[0025] Figure 4 is a schematic diagram of the inner side of a box cover according to an exemplary embodiment;

[0026] Figure 5 is a schematic diagram of a temperature control device according to an exemplary embodiment.

[0027] In the figure:

[0028] 1. Box body; 11. Agitator; 12. Fan; 13. Temperature probe jack; 14. Heating rod jack; 15. Water inlet pipe jack; 16. Faucet;

[0029] 2. Box cover; 21. Observation hole; 22. Fluorescent tube;

[0030] 3. Water quality online monitoring device; 31. Water inlet pipe; 32. Water quality monitoring instrument; 33. Water outlet pipe; 34. Waste liquid barrel;

[0031] 4. Nutrient adding device; 41. Nutrient storage tank; 42. Metering pump; 43. Controller;

[0032] 5. Temperature control device; 51. Digital temperature controller; 52. Temperature probe; 53. Heating rod;

[0033] 6. Removable light shielding plate; 61. Frame; 62. Light shielding sheet. DETAILED DESCRIPTION

[0034] Exemplary embodiments will be described in detail herein, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The implementations described in the following exemplary embodiments do not represent all implementations consistent with the present application. Instead, they are merely examples of devices and methods consistent with some aspects of the present application as detailed in the appended claims.

[0035] The terms used in this application are for the purpose of describing specific embodiments only and are not intended to limit this application. The singular forms of "a", "said" and "the" used in this application and the appended claims are also intended to include plural forms unless the context clearly indicates other meanings. It should also be understood that the term "and / or" used herein refers to and includes any or all possible combinations of one or more associated listed items.

[0036] refer to Figure 1-Figure 5 The present utility model embodiment provides a microorganism cultivation device that can simulate and control multi-dimensional environmental factors, including: an opaque box body 1, a box cover 2, an online water quality monitoring device 3, a nutrient adding device 4, a temperature control device 5, and a detachable light shielding plate 6. The box body 1 has no cover on the top, and an agitator 11 is installed therein, a fan 12 is installed on the side wall, and a temperature probe jack 13, a heating rod jack 14 and a water inlet pipe jack 15 are also opened on the side wall; the box cover 2 is covered on the top of the box body 1, and an observation hole 21 is arranged on the box cover 2, through which the operation of the device can be observed, and a fluorescent tube 22 is installed inside; the water quality online monitoring device 3 includes a water inlet pipe 31, a water quality monitor 21, a water outlet pipe 33 and a waste liquid bucket 34, one end of the water inlet pipe 31 is connected to the water inlet pipe jack 15, and the other end is connected to the water The inlet of the water quality monitor 21 is connected, one end of the water outlet pipe 33 is connected to the outlet of the water quality monitor 21, and the other end is connected to the waste liquid barrel 34; the nutrient adding device 4 includes a plurality of nutrient storage tanks 41, a metering pump 42, and a controller 43, each nutrient storage tank 41 stores different nutrients, and each nutrient storage tank 41 is equipped with a metering pump 42, the input port of the metering pump 42 is connected to the nutrient storage tank 41, and the output port is connected to the box body 1, and all metering pumps 42 are electrically connected to the controller 43; the temperature control device 5 includes a digital display temperature controller 51, a temperature probe 52, and a heating rod 53, the temperature probe 52 is installed on the temperature probe jack 13, the heating rod 53 is installed on the heating rod jack 14, and the digital display temperature controller 51 is electrically connected to the temperature probe 52 and the heating rod 53 respectively. The detachable light shielding plate 6 comprises a frame 61 and a light shielding sheet 62 . The frame 61 is fixed on the box body, and the light shielding sheet 62 is detachably mounted on the frame 61 .

[0037] In order to ensure the stability of the light intensity during the simulation process and not be affected by external light sources, the box body 1 and the box cover 2 are both made of opaque materials. A plurality of daylight jack tubes are installed in parallel on the inner side of the box cover 2 to serve as light sources.

[0038] In order to simulate different light intensities, the removable light-blocking plate 6 is made of a black, opaque material, and the frame 61 is used to support the light-blocking sheet 62; according to the shape and area of ​​the box cover 2, it is evenly divided into several parts, each of which is the size of one light-blocking sheet 62. The number of light-blocking sheets 62 can be installed according to the situation. The light-transmitting area of ​​installing one sheet and installing three sheets is different, so as to control the lighting conditions. When the fluorescent tube 22 is turned on, it can provide a light source for the device. The light-blocking sheet 62 is used to adjust the lighting. After placing the frame, the light-transmitting area can be controlled by increasing or reducing the installation of light-blocking sheets, thereby controlling the amount of light.

[0039] In order to simulate the shear force generated by the flow of water, an agitator 11 is installed on the base, and the agitator 11 may include a motor and a stirring blade installed on the motor shaft, and the stirring blade is located on the base of the box body 1. The stirring blade can adjust the rotation speed, and the motor that drives the stirring blade to rotate is located in the base.

[0040] In order to simulate the natural wind environment, a fan 12 is provided on the box body 1, and the fan 12 can adjust the wind speed.

[0041] In one embodiment, a faucet 16 is also installed on the side wall of the box body 1. The faucet 16 is located at half the height of the box body 1 to facilitate the collection of water samples.

[0042] In one embodiment, a plurality of fluorescent tubes 22 are installed in parallel on the inner side of the box cover 2 .

[0043] In one embodiment, the water quality detector includes a total organic carbon detector, a total phosphorus detector and a total nitrogen detector, because carbon, nitrogen and phosphorus are important nutrients for the growth and reproduction of microorganisms.

[0044] In one embodiment, the light shielding sheet 62 has a plurality of light shielding sheets 62 which are spliced ​​and installed on the frame 61. The light transmission area can be changed by increasing or decreasing the number of light shielding sheets installed, thereby changing the amount of light received by the microorganisms in the culture device.

[0045] In one embodiment, the controller 43 may be a PLC or a single chip microcomputer.

[0046] When in use, a surface water sample is loaded into the box body 1, with the water surface about two centimeters below the fan 12. The fan 12 is used to simulate the natural wind environment, and a variety of wind speeds can be selected. The stirring plate is used to simulate the shear force generated by the flow of water, and multiple speeds can be selected. In order to ensure the stability of the water temperature during the simulation process, the simulated water temperature is set using the digital temperature controller 51. When the water temperature detected by the temperature probe 52 is lower than the set value, the digital temperature controller 51 will control and operate the heating rod 53; when the water temperature detected by the temperature probe 52 is equal to the set temperature, the digital temperature controller 51 will enter a constant temperature control state to achieve a constant water temperature effect; when the water temperature detected by the temperature probe 52 is higher than the set temperature, the digital temperature controller 51 will sound an alarm and stop running.

[0047] The controller 43 is used to set the nutrient concentration level. When the concentration measured by the water quality monitor 21 is lower than the set value, the controller 43 controls the metering pump 42 to start and inject the reagent in the nutrient storage tank 41 into the tank body 1 through the dosing pipe; when the concentration measured by the water quality monitor 21 is equal to or higher than the set value, the controller 43 controls the metering pump 42 to stop running. The waste water detected by the water quality monitor 21 enters the waste liquid barrel 34 through the outlet pipe 33.

[0048] The natural light environment is simulated by using the fluorescent tube 22, and the light intensity is reduced or increased by increasing or decreasing the number of light shielding plates on the frame 61.

[0049] Those skilled in the art will readily appreciate other embodiments of the present application after considering the description and practicing the contents disclosed herein. The present application is intended to cover any modification, use or adaptation of the present application, which follows the general principles of the present application and includes common knowledge or customary techniques in the art that are not disclosed in the present application. The description and examples are intended to be exemplary only, and the true scope and spirit of the present application are indicated by the claims.

[0050] It should be understood that the present application is not limited to the precise structures that have been described above and shown in the drawings, and that various modifications and changes may be made without departing from the scope thereof. The scope of the present application is limited only by the appended claims.

Claims

1. A microbial cultivation device capable of simulating and controlling multi-dimensional environmental factors, characterized in that: include: A light-proof box body, with no cover on the top, an agitator installed inside, a fan installed on the side wall, and a temperature probe jack, a heating rod jack and a water inlet pipe jack on the side wall; A box cover, covering the top of the box body, the box cover is provided with an observation hole, and a fluorescent tube is installed inside; The water quality online monitoring device comprises a water inlet pipe, a water quality monitor, a water outlet pipe and a waste liquid bucket, wherein one end of the water inlet pipe is connected to the water inlet pipe jack, and the other end is connected to the inlet of the water quality monitor, one end of the water outlet pipe is connected to the outlet of the water quality monitor, and the other end is connected to the waste liquid bucket; A nutrient adding device, comprising a plurality of nutrient storage tanks, a metering pump, and a controller, each nutrient storage tank storing different nutrients, each nutrient storage tank being equipped with a metering pump, an input port of the metering pump being connected to the nutrient storage tank, an output port being connected to the box body, and all metering pumps being electrically connected to the controller; The temperature control device comprises a digital temperature controller, a temperature probe, and a heating rod, wherein the temperature probe is mounted on the temperature probe jack, the heating rod is mounted on the heating rod jack, and the digital temperature controller is electrically connected to the temperature probe and the heating rod respectively; The detachable light shielding plate comprises a frame and a light shielding sheet. The frame is fixed on the box body, and the light shielding sheet is detachably mounted on the frame.

2. A microbial cultivation device capable of simulating and controlling multi-dimensional environmental factors according to claim 1, characterized in that: The stirrer comprises a motor and a stirring blade installed on the motor shaft, and the stirring blade is located on the base of the box body.

3. A microorganism cultivation device capable of simulating and controlling multi-dimensional environmental factors according to claim 1, characterized in that: The fan can adjust the wind speed level.

4. A microorganism cultivation device capable of simulating and controlling multi-dimensional environmental factors according to claim 1, characterized in that: A faucet is also installed on the side wall of the box body.

5. A microorganism cultivation device capable of simulating and controlling multi-dimensional environmental factors according to claim 4, characterized in that: The faucet is located at one-half of the height of the box body to facilitate water sample collection.

6. The microorganism cultivation device capable of simulating and controlling multi-dimensional environmental factors according to claim 1, characterized in that: The fluorescent tubes are multiple and are installed in parallel on the inner side of the box cover.

7. The microorganism cultivation device capable of simulating and controlling multi-dimensional environmental factors according to claim 1, characterized in that: The water quality monitor includes a total organic carbon detector, a total phosphorus detector and a total nitrogen detector.

8. The microorganism cultivation device capable of simulating and controlling multi-dimensional environmental factors according to claim 1, characterized in that: The light blocking sheets include a plurality of sheets, and the plurality of light blocking sheets are spliced ​​and installed on the frame.

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