Turnover device for multi-layer cell culture device
By designing a multi-layer cell culture flip device and rotating the multi-layer cell culture using an angle adjustment mechanism, the problem of long-term filling of the culture medium is solved, and the convenience of rapid filling of the culture medium and discharge of cell fluid is achieved.
Patent Information
- Application Number
- CN202420654813.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-01
- Publication Date
- 2025-04-22
- Estimated Expiration
- 2034-04-01
AI Technical Summary
The existing multi-layer cell culturers have slow flow rate due to flat laying during the medium filling process, and it takes a long time to fill each layer.
A multi-layer cell culture device flipping device is designed. By providing a placement frame, a fixing plate, a support frame, a first angle adjustment mechanism and a second angle adjustment mechanism, the multi-layer cell culture device can be rotated from two directions, so that the culture medium can quickly move to the other side of the multi-layer cell culture device and spread each layer.
By rotating the multi-layer cell culturer, the time for the culture medium to fill each layer is significantly accelerated, the efficiency of cell culture is improved, and the discharge of cell fluid is facilitated after the cell culture is completed.
Smart Images
Figure CN222781626U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a turning device for a multi-layer cell culture device. Background Art
[0002] The multilayer cell culture apparatus is a consumable used for large-scale cell culture. The raw material of the cell factory is polystyrene that meets the requirements of USP Class VI. The culture surface is modified to increase the surface hydrophilicity to make the cell culture more uniform. The culture area of a 10-layer multilayer cell culture apparatus is equivalent to 85 T75 cell culture flasks or 36 T175 cell culture flasks. It has a low risk of contamination and can utilize the maximum culture area within a limited space, saving factory space.
[0003] The area of each layer of the multilayer cell culture apparatus is large. After the culture medium is injected from the filling port of the multilayer cell culture apparatus, it is necessary to wait for the culture medium to fill each layer of the multilayer cell culture apparatus. The multilayer cell culture apparatus is usually placed flat, and the speed at which the culture medium flows toward the side away from the filling port is slow. Therefore, it takes a long time for the culture medium to fill each layer of the multilayer cell culture apparatus. Utility Model Content
[0004] In view of the deficiencies of the prior art, the utility model provides a multi-layer cell culture apparatus flipping device, which solves the problems mentioned in the above background.
[0005] The utility model provides the following technical solution: a multi-layer cell culture device flipping device, comprising: a base, a containing frame, a fixing plate, a support frame, a first angle adjustment mechanism and a second angle adjustment mechanism;
[0006] The fixed plate is mounted on the base, the support frame is disposed above the base and is rotatably connected to the fixed plate via the first angle adjustment mechanism, the first angle adjustment mechanism is used to adjust the angle of the support frame from a first direction, the holding frame is rotatably connected to the support frame via the second angle adjustment mechanism, the second angle adjustment mechanism is used to adjust the angle of the holding frame from a second direction, and the holding frame is used to place a multi-layer cell culture apparatus.
[0007] Preferably, the first bolt comprises a first limiting screw handle, a first fixing screw handle, a first bearing, a first connecting shaft and a first connecting flange;
[0008] The first bearing is arranged on a side of the fixing plate facing away from the support frame, one end of the first connecting shaft is fixed to a side of the support frame facing the fixing plate, the other end of the first connecting shaft passes through the fixing plate and is inserted into the first bearing, the first connecting flange is fixedly connected to the first connecting shaft by the first bolt, and the first connecting flange is used to compress and fix the first bearing;
[0009] The fixing plate is provided with a first arc notch, the shaft of the first limiting screw handle passes through the fixing plate through the first arc notch and is fixedly connected to the support frame, and the first arc notch is used to limit the rotation angle of the support frame;
[0010] The fixing plate is provided with a plurality of first threaded holes, the supporting frame is provided with a plurality of first circular holes, the shaft of the first fixing screw handle is provided with threads, the shaft of the first fixing screw handle passes through the first threaded holes of the fixing plate and is inserted into the first circular holes of the supporting frame to fix the position of the supporting frame.
[0011] Preferably, the opening angle of the first circular arc notch is less than 90°, and the center line of the first circular arc notch and the axis of the first bearing are located on the same horizontal plane.
[0012] Preferably, the second bolt, the second limiting screw handle, the second fixing screw handle, the second bearing, the second connecting shaft and the second connecting flange;
[0013] The second bearing is arranged on a side of the support frame facing away from the containing frame, one end of the second connecting shaft is fixed to a side of the containing frame facing the support frame, the other end of the second connecting shaft passes through the support frame and is inserted into the second bearing, the second connecting flange is fixedly connected to the second connecting shaft by the second bolt, and the second connecting flange is used to compress and fix the second bearing;
[0014] A second arc notch is provided on the containing frame, and the shaft of the second limiting screw handle passes through the supporting frame and is inserted into the second arc notch, and the second arc notch is used to limit the rotation angle of the containing frame;
[0015] The support frame is provided with a plurality of second threaded holes, the containing frame is provided with a plurality of second circular holes, the shaft of the second fixed screw handle is provided with threads, the shaft of the second fixed screw handle passes through the threaded holes of the support frame and is inserted into the second circular holes of the containing frame to fix the position of the containing frame.
[0016] Preferably, the opening angle of the second circular arc notch is between 90° and 180°, and the center line of the second circular arc notch is lower than the axis of the second bearing.
[0017] Preferably, the support frame is a U-shaped structure, and mounting grooves are provided on opposite sides of the support frame. Two second angle adjustment mechanisms are provided, and the two second angle adjustment mechanisms are respectively installed in the two mounting grooves of the support frame.
[0018] Preferably, the method further comprises: a fixing component, wherein the fixing component is hinged to the open end of the containing frame and is used for fixing the multi-layer cell culture device in the containing frame.
[0019] Preferably, the fixing assembly comprises: a fixing arm, a snap lock and a hinge;
[0020] The fixed arm is an L-shaped structure, the hinge is installed at one end of the fixed arm, the buckle lock is installed at the other end of the fixed arm, the fixed arm is rotatably connected to the containing frame through the hinge, and the fixed arm is fixed to the bottom of the containing frame through the buckle lock.
[0021] Preferably, a first handle is installed on the fixed arm, and second handles are installed on the surface of the containing frame and both sides of the support frame, and the first handle is arranged opposite to the second handle on the surface of the containing frame.
[0022] Preferably, universal wheels are installed at the four corners of the bottom of the base.
[0023] Compared with the prior art, the utility model has the following beneficial effects:
[0024] 1. The multi-layer cell culture apparatus flipping device is provided with a holding frame, a fixing plate, a supporting frame, a first angle adjustment mechanism and a second angle adjustment mechanism. The multi-layer cell culture apparatus is placed in the holding frame, and the first angle adjustment mechanism and the second angle adjustment mechanism can be used to rotate the multi-layer cell culture apparatus in two directions. After the culture medium is injected from the filling port of the multi-layer cell culture apparatus, the multi-layer cell culture apparatus is rotated and shaken, so that the culture medium can be quickly moved to the other side of the multi-layer cell culture apparatus and spread all over each layer of the multi-layer cell culture apparatus. In addition, after the cell culture is completed, the multi-layer cell culture apparatus is rotated so that the filling port and the discharge port can be changed from a horizontal direction to a downward tilt, so as to facilitate the discharge of the cell fluid.
[0025] 2. The multi-layer cell culture apparatus flipping device is provided with a fixed arm, a buckle lock, a hinge and a limit part. After the multi-layer cell culture apparatus is placed in the interior of the containing frame, the fixed arm is rotated to buckle the multi-layer cell culture apparatus, and the position of the fixed arm is locked with each buckle lock and the limit part. The multi-layer cell culture apparatus is fixed by the fixed arm to prevent the multi-layer cell culture apparatus from being separated from the containing frame when being shaken. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;
[0027] Figure 2 This is a schematic diagram of the front view structure of the utility model;
[0028] Figure 3 A schematic diagram of the structure of the utility model from top view;
[0029] Figure 4 For this utility model Figure 2 Schematic diagram of the side section structure at the position of the first angle adjustment mechanism.
[0030] Figure 5 This is a side view of the structure of the utility model;
[0031] In the figure: 1. multilayer cell culture apparatus; 2. base; 3. holding frame; 4. fixing plate; 5. support frame; 6. first angle adjustment mechanism; 61. first bolt; 62. first limiting screw handle; 63. first fixing screw handle; 64. bearing; 65. first connecting shaft; 66. first connecting flange; 7. second angle adjustment mechanism; 71. second bolt; 72. second limiting screw handle; 73. second fixing screw handle; 8. fixing assembly; 81. fixing arm; 82. buckle lock; 83. hinge; 84. first handle; 9. limiting part; 10. second handle; 11. universal wheel; 12. first arc notch; 13. second arc notch; 14. mounting groove. DETAILED DESCRIPTION
[0032] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0033] See also Figure 1 The multi-layer cell culture device flipping device includes: a base 2, a holding frame 3, a fixing plate 4, a support frame 5, a first angle adjustment mechanism 6 and a second angle adjustment mechanism 7; the fixing plate 4 is installed on the base 2, and universal wheels 11 are installed at the four corners of the bottom of the base 2, and brake pads are provided on the universal wheels 11. The support frame 5 is arranged above the base 2 and is rotatably connected to the fixing plate 4 through the first angle adjustment mechanism 6. The first angle adjustment mechanism 6 is used to adjust the angle of the support frame 5 from a first direction. The holding frame 3 is rotatably connected to the support frame 5 through the second angle adjustment mechanism 7. The second angle adjustment mechanism 7 is used to adjust the angle of the holding frame 3 from a second direction. The holding frame 3 is used to place the multi-layer cell culture device 1.
[0034] See also Figure 2-4The first angle adjustment mechanism 6 includes: a first bolt 61, a first limiting screw handle 62, a first fixing screw handle 63, a first bearing 64, a first connecting shaft 65 and a first connecting flange 66. The first bearing 64 is arranged on the side of the fixing plate 4 facing away from the support frame 5. One end of the first connecting shaft 65 is fixed to the side of the support frame 5 facing the fixing plate 4. The other end of the first connecting shaft 65 passes through the fixing plate 4 and is inserted into the first bearing 64. The first connecting flange 66 is fixedly connected to the first connecting shaft 65 by the first bolt 61. The first connecting flange 66 is used to press and fix the first bearing 64.
[0035] Preferably, the first limiting screw handle 62 and the first fixing screw handle 63 are symmetrically arranged with the first bearing 64 as the center.
[0036] A first arc groove 12 is provided on the fixing plate 4, and the shaft of the first limiting screw handle 62 passes through the fixing plate 4 via the first arc groove 12 and is fixedly connected to the support frame 5. The first arc groove 12 is used to limit the rotation angle of the support frame 5. A plurality of first threaded holes are provided on the fixing plate 4, and a plurality of first circular holes are provided on the support frame 5. The shaft of the first fixed screw handle 63 is provided with threads, and the shaft of the first fixed screw handle 63 passes through the first threaded hole of the fixing plate 4 and is inserted into the first circular hole of the support frame 5 to fix the position of the support frame 5.
[0037] The opening angle of the first arc slot 12 is less than 90°, and the center line of the first arc slot 12 and the axis of the first bearing 64 are located on the same horizontal plane.
[0038] In a specific embodiment, the opening angle of the first arc notch 12 is 60°, and the number of threaded holes of the fixing plate 4 is 3, so that the position of the supporting frame 5 can be fixed every 30°.
[0039] exist Figure 2 In the embodiment, the support frame 5 is at the 0° position. The support frame 5 can swing 30° to the left and right, and the fixing plate 4 has three threaded holes, which correspond to the center position (ie, the 0° position) and the left and right extreme positions (ie, the 30° and -30° positions).
[0040] See also Figure 5 The second angle adjustment mechanism 7 includes: a second bolt 71, a second limiting screw handle 72, a second fixing screw handle 73, a second bearing, a second connecting shaft and a second connecting flange. The second bearing is arranged on the side of the support frame 5 facing away from the containing frame 3. One end of the second connecting shaft is fixed to the side of the containing frame 3 facing the support frame 5. The other end of the second connecting shaft passes through the support frame 5 and is inserted into the second bearing. The second connecting flange is fixedly connected to the second connecting shaft by the second bolt 71. The second connecting flange is used to press and fix the second bearing.
[0041] A second arc notch 13 is provided on the containing frame 3, and the shaft of the second limiting screw handle 72 passes through the support frame 5 and is inserted into the second arc notch 13. The second arc notch 13 is used to limit the rotation angle of the containing frame 3. A plurality of second threaded holes are provided on the supporting frame 5, and a plurality of second circular holes are provided on the containing frame 3. A thread is provided on the shaft of the second fixing screw handle 73, and the shaft of the second fixing screw handle 73 passes through the threaded hole of the supporting frame 5 and is inserted into the second circular hole of the containing frame 3 to fix the position of the containing frame 3.
[0042] Preferably, the second limiting screw handle 72 and the second fixing screw handle 73 are symmetrically arranged with the second bearing as the center.
[0043] The opening angle of the second arc slot 13 is between 90° and 180°, and the center line of the second arc slot 13 is lower than the axis of the second bearing.
[0044] In a specific embodiment, the opening angle of the second arc notch 13 is 150°, and the number of second threaded holes of the support frame 5 is 6, which can fix the position of the containing frame 3 at intervals of 30°.
[0045] exist Figure 5 In the embodiment, the holding frame 3 is at the 0° position, the filling inlet and the discharge outlet of the multilayer cell culture apparatus are both vertically upward, and the filling inlet and the discharge outlet are used to inject culture medium or discharge cultured cell fluid, and the holding frame 3 can swing 30° to the left and 120° to the right. When the rightward swing exceeds 90°, the filling inlet and the discharge outlet of the multilayer cell culture apparatus are changed from the horizontal direction to the downward tilt, so as to facilitate the discharge of the cell fluid;
[0046] The support frame 5 is a U-shaped structure, and mounting grooves 14 are provided on opposite sides of the support frame 5. Two second angle adjustment mechanisms 7 are provided, and the two second angle adjustment mechanisms 7 are respectively installed in the two mounting grooves 14 of the support frame 5. The base 2 and the support frame 5 are both U-shaped structures and have the same opening direction.
[0047] It also includes: a fixing assembly 8, which is hinged to the open end of the holding frame 3 and is used to fix the multi-layer cell culture device 1 in the holding frame 3. The fixing assembly 8 includes: a fixing arm 81, a buckle lock 82 and a hinge 83. The fixing arm 81 is an L-shaped structure. The hinge 83 is installed at one end of the fixing arm 81, and the buckle lock 82 is installed at the other end of the fixing arm 81. The fixing arm 81 is rotatably connected to the holding frame 3 through the hinge 83, and the fixing arm 81 is fixed to the bottom of the holding frame 3 through the buckle lock 82.
[0048] Specifically, a limiting portion 9 is provided at the bottom of the containing frame 3 , and the limiting portion 9 is cooperatively connected with the buckle lock 82 .
[0049] A first handle 84 is installed on the fixed arm 81, and second handles 10 are installed on the surface of the containing frame 3 and both sides of the supporting frame 5. The first handle 84 is arranged opposite to the second handle 10 on the surface of the containing frame 3, providing a gripping position when installing the containing frame 3 and the supporting frame 5, facilitating installation.
[0050] The multilayer cell culture apparatus 1 is placed into the containing frame 3, with the filling port and the discharge port of the multilayer cell culture apparatus 1 facing upwards. After the multilayer cell culture apparatus 1 is placed inside the containing frame 3, the fixing arm 81 is rotated to buckle the multilayer cell culture apparatus 1, and the position of the fixing arm 81 is locked by the buckle lock 82 and the limiting part 9. The multilayer cell culture apparatus 1 is fixed by the fixing arm 81 to prevent the multilayer cell culture apparatus 1 from escaping from the containing frame 3 when being shaken.
[0051] After the multi-layer cell culture device 1 is fixed, the spiral handle of the second angle adjustment mechanism 7 is rotated to drive the containing frame 3 to rotate. The containing frame 3 drives the multi-layer cell culture device 1 to rotate, so that the filling inlet and the discharge outlet of the multi-layer cell culture device 1 can be turned to the side away from the fixed plate 4. In this process, the second arc groove 13 cooperates with the second limiting spiral handle 72 to limit and guide the rotation trajectory of the containing frame 3. After the containing frame 3 is rotated into place, the second fixing spiral handle 73 is rotated and inserted into the circular hole of the containing frame 3 to fix the relative position of the containing frame 3 and the supporting frame 5. Rotating the spiral handle of the first angle adjustment mechanism 6 can drive the support frame 5 to move, and the support frame 5 drives the multi-layer cell culture device 1 to be tilted and shaken 30 degrees up and down in the horizontal direction, so that the cell culture solution can quickly move to the other side of the multi-layer cell culture device 1 and cover each layer of the multi-layer cell culture device 1. Similarly, in this process, the first arc groove 12 cooperates with the first limiting spiral handle 62 to limit and guide the rotation trajectory of the support frame 5. After the support frame 5 is rotated into place, the first fixing spiral handle 63 is rotated and inserted into the circular hole of the support frame 5, so as to fix the relative position of the support frame 5 and the fixing plate 4.
[0052] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A multi-layer cell culture apparatus flipping device, characterized in that: include: A base (2), a containing frame (3), a fixing plate (4), a supporting frame (5), a first angle adjustment mechanism (6) and a second angle adjustment mechanism (7); The fixing plate (4) is mounted on the base (2); the supporting frame (5) is arranged above the base (2) and is rotatably connected to the fixing plate (4) via the first angle adjustment mechanism (6); the first angle adjustment mechanism (6) is used to adjust the angle of the supporting frame (5) from a first direction; the containing frame (3) is rotatably connected to the supporting frame (5) via the second angle adjustment mechanism (7); the second angle adjustment mechanism (7) is used to adjust the angle of the containing frame (3) from a second direction; the containing frame (3) is used to place the multi-layer cell culture device (1).
2. The multi-layer cell culture apparatus turning device according to claim 1, characterized in that: The first angle adjustment mechanism (6) comprises: a first bolt (61), a first limiting screw handle (62), a first fixing screw handle (63), a first bearing (64), a first connecting shaft (65) and a first connecting flange (66); The first bearing (64) is arranged on a side of the fixing plate (4) facing away from the support frame (5); one end of the first connecting shaft (65) is fixed to a side of the support frame (5) facing the fixing plate (4); the other end of the first connecting shaft (65) passes through the fixing plate (4) and is inserted into the first bearing (64); the first connecting flange (66) is fixedly connected to the first connecting shaft (65) by the first bolt (61); and the first connecting flange (66) is used to press and fix the first bearing (64); The fixing plate (4) is provided with a first arc notch (12), and the shaft of the first limiting screw handle (62) passes through the fixing plate (4) via the first arc notch (12) and is fixedly connected to the support frame (5), and the first arc notch (12) is used to limit the rotation angle of the support frame (5); The fixing plate (4) is provided with a plurality of first threaded holes, the supporting frame (5) is provided with a plurality of first circular holes, the shaft of the first fixing screw handle (63) is provided with threads, the shaft of the first fixing screw handle (63) passes through the first threaded holes of the fixing plate (4) and is inserted into the first circular holes of the supporting frame (5) to fix the position of the supporting frame (5).
3. The multi-layer cell culture apparatus turning device according to claim 2, characterized in that: The opening angle of the first arc notch (12) is less than 90°, and the center line of the first arc notch (12) and the axis of the first bearing (64) are located on the same horizontal plane.
4. The multi-layer cell culture apparatus turning device according to claim 1, characterized in that: The second angle adjustment mechanism (7) comprises: a second bolt (71), a second limiting screw handle (72), a second fixing screw handle (73), a second bearing, a second connecting shaft and a second connecting flange; The second bearing is arranged on a side of the support frame (5) facing away from the containing frame (3), one end of the second connecting shaft is fixed to a side of the containing frame (3) facing the support frame (5), the other end of the second connecting shaft passes through the support frame (5) and is inserted into the second bearing, the second connecting flange is fixedly connected to the second connecting shaft by the second bolt (71), and the second connecting flange is used to press and fix the second bearing; The containing frame (3) is provided with a second circular arc notch (13), the shaft of the second limiting screw handle (72) passes through the support frame (5) and is inserted into the second circular arc notch (13), and the second circular arc notch (13) is used to limit the rotation angle of the containing frame (3); The support frame (5) is provided with a plurality of second threaded holes, the containing frame (3) is provided with a plurality of second circular holes, the shaft of the second fixed screw handle (73) is provided with threads, the shaft of the second fixed screw handle (73) passes through the threaded holes of the support frame (5) and is inserted into the second circular holes of the containing frame (3) to fix the position of the containing frame (3).
5. The multi-layer cell culture apparatus turning device according to claim 4, characterized in that: The opening angle of the second circular arc slot (13) is between 90° and 180°, and the center line of the second circular arc slot (13) is lower than the axis of the second bearing.
6. The multi-layer cell culture apparatus turning device according to claim 1, characterized in that: The support frame (5) is a U-shaped structure, and mounting grooves (14) are provided on opposite sides of the support frame (5). Two second angle adjustment mechanisms (7) are provided, and the two second angle adjustment mechanisms (7) are respectively installed in the two mounting grooves (14) of the support frame (5).
7. The multi-layer cell culture apparatus turning device according to claim 1, characterized in that: Also includes: A fixing component (8), the fixing component (8) is hinged to the open end of the containing frame (3) and is used to fix the multi-layer cell culture device (1) in the containing frame (3).
8. The multi-layer cell culture apparatus turning device according to claim 7, characterized in that: The fixing assembly (8) comprises: a fixing arm (81), a buckle lock (82) and a hinge (83); The fixed arm (81) is an L-shaped structure, the hinge (83) is installed at one end of the fixed arm (81), the buckle lock (82) is installed at the other end of the fixed arm (81), the fixed arm (81) is rotatably connected to the containing frame (3) through the hinge (83), and the fixed arm (81) is fixed to the bottom of the containing frame (3) through the buckle lock (82).
9. The multi-layer cell culture apparatus turning device according to claim 8, characterized in that: A first handle (84) is installed on the fixed arm (81), and second handles (10) are installed on the surface of the containing frame (3) and on both sides of the support frame (5), and the first handle (84) is arranged opposite to the second handle (10) on the surface of the containing frame (3).
10. The multi-layer cell culture vessel turning device according to claim 1, characterized in that: Universal wheels (11) are installed at the four corners of the bottom of the base (2).