Liquid correcting device

Abstract

The utility model provides a liquid correcting device. The liquid correcting device comprises a carrier platform, a measuring module and a movement mechanism electrically connected with a central control module. The carrier platform fixedly bears a 96-well plate, and the 96-well plate is used for receiving a to-be-tested reagent obtained by liquid beating of a steel needle of an Oligo synthesizer located below; the measuring module is mounted below the movement mechanism; the central control module is used for driving the movement mechanism to move, and the measurement module traverses the measurement of all the to-be-tested reagents in the 96-well plate under the movement of the movement mechanism. The utility model discloses an Oligo synthesizer liquid calibration device, and aims to accurately measure the actual reagent volume of a trace reagent and further determine the steel needle distribution performance of an Oligo synthesizer in combination with the distribution amount of a steel needle of the synthesizer so as to realize liquid calibration of the Oligo synthesizer.

Description

Technical Field

[0001] The utility model relates to the technical field of molecular biology, in particular to a liquid calibration device. Background Art

[0002] Oligo synthesizer is an instrument for automatically synthesizing short-fragment oligonucleotides. It is mainly used in the field of DNA and RNA synthesis. It is one of the indispensable and most widely used equipment in the synthesis field.

[0003] The synthesis quality of Oligo synthesizers on the market varies greatly. The synthesis quality of different equipment varies. The reason is that Oligo synthesizers need to be calibrated by professionals before use, that is, to calibrate the accuracy of the amount of reagents dispensed each time. The amount of liquid used for normal synthesis is between 5 microliters and 10 milliliters, and the synthesis quality can be guaranteed within a deviation of 5%. However, there is currently no unified method and equipment for standardized operation in terms of liquid volume calibration. Manual calibration is used entirely, and the calibration results of different personnel depend entirely on experience accumulation. Utility Model Content

[0004] In view of the above-mentioned deficiencies or defects in the prior art, the utility model provides a liquid calibration device, which aims to accurately measure the actual reagent volume of trace reagents, and then determine the steel needle distribution performance of the Oligo synthesizer in combination with the distribution amount of the synthesizer steel needle, thereby realizing the liquid calibration of the Oligo synthesizer.

[0005] To achieve the above-mentioned purpose, the utility model provides a liquid calibration device, comprising: a carrier platform, a measuring module, and a motion mechanism electrically connected to a central control module; the carrier platform fixedly supports a 96-well plate, and the 96-well plate is used to receive the test reagent obtained by the steel needle of an Oligo synthesizer located below; the measuring module is installed under the motion mechanism; the central control module is used to drive the movement of the motion mechanism, and the measuring module traverses the measurement of all the test reagents in the 96-well plate under the movement of the motion mechanism.

[0006] Optionally, the motion mechanism includes: an X-axis frame, a Y-axis frame, and a Z-axis frame; the Z-axis frame moves along the X-axis on the X-axis frame through the X-axis motor drive; the Y-axis frame moves along the Z-axis on the Z-axis frame through the Z-axis motor drive, and moves perpendicular to the X-axis through the Y-axis motor drive.

[0007] Optionally, the measurement module is composed of 6 micro-volume measuring instruments, and the distance between adjacent micro-volume measuring instruments is two wells of the 96-well plate.

[0008] Optionally, the micro-volume measuring instrument includes: a motor, a plunger and a capillary installed on a screw from top to bottom; the top end of the screw is fixedly installed on the motion mechanism, and the motor drives the plunger to move up and down through the screw; during the measurement process, the capillary is inserted into the test agent, and the plunger is driven up by the motor to extract the test agent into the capillary.

[0009] Optionally, a photoelectric sensor is installed on the capillary.

[0010] Optionally, the central control module includes: an electrical drive component and a motor pulse control component connected to a programmable controller.

[0011] Optionally, the electrical drive assembly includes: an X-axis drive unit connected to the X-axis motor, a Y-axis drive unit connected to the Y-axis motor, and a Z-axis drive unit connected to the Z-axis motor.

[0012] Optionally, the motor includes an encoder and a stepper motor; the motor pulse control component includes: a feedback unit connected to the encoder, and a motor drive component connected to the stepper motor.

[0013] Optionally, the central control module further comprises: a switch quantity input component connected to the programmable controller, and the switch quantity input component is connected to the photoelectric sensor.

[0014] Optionally, the programmable controller is equipped with an Ethernet port, and the Ethernet port can be communicatively connected to an Oligo synthesizer device.

[0015] It can be seen from the above technical solutions that the utility model has the following advantages:

[0016] The utility model discloses a liquid calibration device, comprising: a carrier platform, a measuring module, and a motion mechanism electrically connected to a central control module; the carrier platform fixedly carries a 96-well plate, the 96-well plate is used to receive a test reagent obtained by injecting liquid with a steel needle of an Oligo synthesizer located below; the measuring module is installed under the motion mechanism; the central control module is used to drive the movement of the motion mechanism, and the measuring module traverses the measurement of all the test reagents in the 96-well plate under the movement of the motion mechanism.

[0017] By driving the movement of the motion mechanism through the central control module, the measurement module can automatically traverse and measure all the test reagents in the 96-well plate, realizing a highly automated and efficient test process. At the same time, the motion mechanism can accurately move to each well position to ensure that the measurement module can accurately measure each test reagent, thereby improving the accuracy of the test results. The actual reagent volume obtained by accurate measurement is combined with the distribution volume of the synthesizer steel needle to determine the steel needle distribution performance of the Oligo synthesizer, thereby realizing the calibration of the Oligo synthesizer.

[0018] In addition, the carrier platform can flexibly replace different sample plates for testing, which is suitable for a variety of experimental needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0020] Figure 1 It is a schematic diagram of the composition of a liquid calibration device of the utility model;

[0021] Figure 2 This is a schematic diagram of a 96-well plate of a liquid calibration device of the utility model;

[0022] Figure 3 It is a schematic diagram of initial measurement of a micro-volume measuring instrument of a liquid calibration device of the utility model;

[0023] Figure 4 This is a schematic diagram of information interaction between a micro-volume measuring instrument and a central control module of a liquid calibration device of the utility model;

[0024] Figure 5 This is a schematic diagram of the central module composition of a liquid calibration device of the utility model;

[0025] Figure 6 The utility model is a schematic diagram of the arrangement of 96 well plates of a liquid calibration device. DETAILED DESCRIPTION

[0026] The present disclosure is further described below in conjunction with the embodiments shown in the accompanying drawings.

[0027] The embodiment of the utility model provides a material flow detection device, which solves the technical problem that it is difficult to strike a balance between the detection accuracy and the installation accuracy of the detection device in the existing material flow detection devices.

[0028] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0029] In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0030] Unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0031] For easier understanding, see Figure 1 , Figure 1 This is a schematic diagram of the composition of a liquid calibration device of the present invention. The liquid calibration device in the embodiment of the present invention includes: a carrier platform 4, a measuring module, and a motion mechanism electrically connected to the central control module 1; the carrier platform fixedly supports a 96-well plate, and the 96-well plate is used to receive the test reagent obtained by the Oligo synthesizer steel needle located below; the measuring module is installed under the motion mechanism; the central control module 1 is used to drive the movement of the motion mechanism, and the measuring module traverses the measurement of all the test reagents in the 96-well plate under the movement of the motion mechanism.

[0032] The liquid calibration device in the embodiment of the utility model comprises: a fixed support such as Figure 2The 96-well plate shown has a carrier platform 4 for receiving the test agent; it is installed under the motion mechanism and is used to measure the test agent in the 96-well plate, and during the measurement process, a measurement module can be inserted into each well for measurement; it is controlled by the central control module 1 and can move up and down, left and right, helping the measurement module to traverse the motion mechanism of all the test agents in the 96-well plate. In this way, a large number of test agents from the Oligo synthesizer steel needles can be efficiently measured, and the Oligo synthesizer can be calibrated.

[0033] Specifically, the motion mechanism includes: an X-axis frame 21, a Y-axis frame 23, and a Z-axis frame 22; the Z-axis frame 22 is driven by the X-axis motor 11 on the X-axis frame 21 to move along the X-axis; the Y-axis frame 23 is driven by the Z-axis motor 12 on the Z-axis frame 22 to move along the Z-axis, and is driven by the Y-axis motor 13 to move vertically along the X-axis.

[0034] In an embodiment of the utility model, the motion mechanism is a universal X / Y / Z cantilever module, wherein a Z-axis frame 22 is installed on an X-axis frame 21, and is driven by an X-axis motor 11 to move along the X-axis direction, thereby controlling the movement of the motion mechanism in the horizontal direction. The Y-axis frame 23 is installed on the Z-axis frame 22, and is driven by the Y-axis motor 13 to move perpendicular to the X-axis direction, so that the motion mechanism can move in a direction perpendicular to the X-axis. The Z-axis frame 22 is installed on the X-axis frame 21, and is driven by the Z-axis motor 12 to move along the Z-axis direction, controlling the movement of the motion mechanism in a direction perpendicular to the carrier platform 4. Through the movement of the X-axis, Y-axis and Z-axis, precise control and movement of the motion mechanism in three directions are achieved, thereby achieving comprehensive measurement of the test agent in the 96-well plate through the measurement module.

[0035] Specifically, the measurement module is composed of 6 micro-volume measuring instruments, and the distance between adjacent micro-volume measuring instruments is two wells of the 96-well plate.

[0036] In this new embodiment, the measuring machine is composed of 6 micro-volume measuring instruments, and the spacing between each micro-volume measuring instrument is exactly the spacing between 2 wells of the 96-well plate. Under this spacing, the 6 micro-volume measuring instruments can complete the measurement of all 96 wells by moving horizontally once and vertically 8*2=16 times. The initial positions of the 6 micro-volume measuring instruments and the 96-well plate are as follows: Figure 3 The initial measurement schematic of the microvolume measuring instrument is shown in FIG.

[0037] See also Figure 3 and Figure 4 , Figure 4As shown in the schematic diagram of information interaction between the micro-volume measuring instrument and the central control module, specifically, the micro-volume measuring instrument includes: a motor, a plunger 34 and a capillary 35 installed on a screw 31 from top to bottom; the top end of the screw 31 is fixedly installed on the motion mechanism, and the motor drives the plunger 34 to move up and down through the screw 31; during the measurement process, the capillary 35 is inserted into the test agent, and the plunger 34 is driven up by the motor to extract the test agent into the capillary 35.

[0038] In the embodiment of the utility model, during the measurement process, the capillary 35 is inserted into the test agent, and the motor drives the screw 31 to move the plunger 34, and when the motor drives the plunger 34 to rise, the test agent can be drawn into the capillary 35 to complete the extraction of the test agent.

[0039] See also Figure 4 Specifically, a photoelectric sensor 36 is installed on the capillary 35 .

[0040] In the embodiment of the utility model, a high-precision photoelectric sensor is installed on the capillary 35 to sense whether the liquid to be tested still exists in the capillary 35 .

[0041] See also Figure 4 and Figure 5 , Figure 5 This is a schematic diagram of the central module composition. The central control module 1 includes: an electrical drive component and a motor pulse control component connected to a programmable controller 17.

[0042] In the embodiment of the utility model, the motor pulse control component is connected to the programmable controller 17 to control the pulse signal of the motor in the micro-measurement instrument to achieve accurate motor movement and position control. The electrical drive component is connected to the programmable controller 17 to control the motor movement and position control of the X-axis motor 11, the Y-axis motor 13 and the Z-axis motor 12 of the motion mechanism.

[0043] Please continue reading Figure 4 and Figure 5 The central control module 1 also includes: a switch input component 16 connected to the programmable controller 16 , and the switch input component 16 is connected to the photoelectric sensor 36 .

[0044] In the measurement process of the embodiment of the utility model, when the photoelectric sensor 36 detects that there is no more liquid to be measured in the capillary 35, the switch input component 16 transmits a switch signal to the central control module 1. The switch signal is a Boolean signal.

[0045] It should be noted that Boolean signals usually refer to logical signals, which have only two possible states: True or False. In control systems, Boolean signals are usually used to represent switch states or logical conditions, such as whether a switch is on or off, or the trigger state of a sensor.

[0046] Please continue reading Figure 4 and Figure 5 The motor includes an encoder 32 and a stepper motor 33 ; the motor pulse control component includes: a feedback unit 14 connected to the encoder 32 , and a motor drive component 15 connected to the stepper motor 33 .

[0047] In the embodiment of the utility model, further, the motor includes an encoder 32 and a stepper motor 33, and the click pulse control component includes a feedback unit 14 and an electrical drive component 15. The feedback unit 14 can be used to receive the feedback signal of the encoder 32 to determine the position or speed of the stepper motor 33, and then control the operation of the stepper motor 33 through the motor drive component 15.

[0048] See also Figure 5 The electrical drive assembly includes: an X-axis drive unit 18 connected to the X-axis motor 11, a Y-axis drive unit 19 connected to the Y-axis motor 13, and a Z-axis drive unit 20 connected to the Z-axis motor 12.

[0049] In the embodiment of the present utility model, the electrical drive assembly includes an X-axis drive unit 18, a Y-axis drive unit 19 and a Z-axis drive unit 20. These drive units may be used to control the operation of each axial motor to achieve positioning or movement of the motion mechanism.

[0050] Please continue reading Figure 5 The programmable controller 17 is equipped with an Ethernet port, and the Ethernet port can be connected to the Oligo synthesizer device 5 for communication.

[0051] In the embodiment of the utility model, the programmable controller 17 is provided with an Ethernet port, and the measured data can be directly transmitted to the Oligo synthesizer device 5 for calibration through communication.

[0052] Oligo synthesizer equipment is usually equipped with 6-20 sets of liquid injection steel needles, which are used to inject 6-20 reagents respectively. Each set of steel needles contains 8 needles and can inject 8 holes at the same time. Taking 12 sets of liquid injection steel needles as an example, the arrangement diagram is as follows Figure 6 During the manual calibration of the synthesizer, a 96-well plate is usually placed under the steel needle, the needle injection time is set to receive the reagent, and then the reagent volume is manually measured using a pipette, and the data is entered into the software for curve calculation.

[0053] Each set of needles represents a reagent, but it is also possible to use a single needle for injection. Therefore, each needle needs to be calibrated individually and each set of needles needs to be calibrated. Each needle needs to be calibrated individually 96 times, and the combination needs to be calibrated 12 times, for a total of 108 times. At normal operating speed, each calibration takes about 3 minutes. Therefore, the total calibration time is 108*3 / 60=5.4 hours.

[0054] In addition, the calibration process may encounter inaccuracies or abnormalities. At this time, it is necessary to check the pipeline, solenoid valve and other problems, and re-calibrate the liquid after eliminating the abnormality. Usually, the liquid calibration process takes a whole day, and the stability of the liquid calibration of the equipment between different batches cannot be guaranteed.

[0055] In order to solve the above-mentioned problems, the utility model discloses a liquid calibration device, comprising: a carrier platform, a measuring module, and a motion mechanism electrically connected to a central control module; the carrier platform fixedly carries a 96-well plate, and the 96-well plate is used to receive the test reagents obtained by the steel needle of an Oligo synthesizer located below; the measuring module is installed under the motion mechanism; the central control module is used to drive the movement of the motion mechanism, and the measuring module traverses the measurement of all the test reagents in the 96-well plate under the movement of the motion mechanism.

[0056] By driving the movement of the motion mechanism through the central control module, the measurement module can automatically traverse and measure all the test reagents in the 96-well plate, realizing a highly automated and efficient test process. At the same time, the motion mechanism can accurately move to each well position to ensure that the measurement module can accurately measure each test reagent, thereby improving the accuracy of the test results. The actual reagent volume obtained by accurate measurement is combined with the distribution volume of the synthesizer steel needle to determine the steel needle distribution performance of the Oligo synthesizer, thereby realizing the calibration of the Oligo synthesizer.

[0057] In addition, the carrier platform can flexibly replace different sample plates for testing, which is suitable for a variety of experimental needs.

[0058] As described above, the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A liquid calibration device, characterized in that: include: A carrier platform, a measuring module, and a motion mechanism electrically connected to a central control module; the carrier platform fixedly carries a 96-well plate, the 96-well plate is used to receive the test reagents obtained by the steel needle of an Oligo synthesizer located below; the measuring module is installed under the motion mechanism; the central control module is used to drive the movement of the motion mechanism, and the measuring module traverses the measurement of all the test reagents in the 96-well plate under the movement of the motion mechanism.

2. The liquid calibration device according to claim 1, characterized in that: The motion mechanism includes: an X-axis frame, a Y-axis frame, and a Z-axis frame; the Z-axis frame is driven by an X-axis motor to move along the X-axis on the X-axis frame; the Y-axis frame is driven by a Z-axis motor to move along the Z-axis on the Z-axis frame, and is driven by a Y-axis motor to move perpendicular to the X-axis.

3. The liquid calibration device according to claim 2, characterized in that: The measuring module is composed of 6 micro-volume measuring instruments, and the distance between adjacent micro-volume measuring instruments is equal to two wells of the 96-well plate.

4. The liquid calibration device according to claim 3, characterized in that: The micro-volume measuring instrument comprises: a motor, a plunger and a capillary which are installed on a screw from top to bottom; the top end of the screw is fixedly installed on the motion mechanism, and the motor drives the plunger to move up and down through the screw; during the measurement process, the capillary is inserted into the test agent, and the motor drives the plunger to rise, so that the test agent is extracted into the capillary.

5. The liquid calibration device according to claim 4, characterized in that: A photoelectric sensor is installed on the capillary.

6. The liquid calibration device according to claim 5, characterized in that: The central control module includes: an electrical drive component and a motor pulse control component connected to a programmable controller.

7. The liquid calibration device according to claim 6, characterized in that: The electrical drive assembly includes: an X-axis drive unit connected to the X-axis motor, a Y-axis drive unit connected to the Y-axis motor, and a Z-axis drive unit connected to the Z-axis motor.

8. The liquid calibration device according to claim 6, characterized in that: The motor includes an encoder and a stepper motor; the motor pulse control component includes: a feedback unit connected to the encoder, and a motor drive component connected to the stepper motor.

9. The liquid calibration device according to claim 6, characterized in that: The central control module further comprises: a switch quantity input component connected to the programmable controller, and the switch quantity input component is connected to the photoelectric sensor.

10. The liquid calibration device according to claim 6, characterized in that: The programmable controller is equipped with an Ethernet port, and the Ethernet port can be connected to the Oligo synthesizer device for communication.

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