Two-photon fluorescent multicolor imaging device

Abstract

The utility model discloses a two-photon fluorescent multicolor imaging device, which belongs to the technical field of imaging and is characterized by comprising a placing plate and a switching mechanism, the right side of the placing plate is bolted with a computer, and the placing plate and the switching mechanism are arranged, so that biological samples can be supported by the placing plate, and the switching mechanism is arranged on the placing plate. The computer can be matched with the two-photon fluorescent multicolor imager body to carry out imaging processing on a biological sample, the fixing plate can limit the two-photon fluorescent multicolor imager body and the servo motor, the servo motor can be matched with the switching rotating rod to drive the fixing shell to carry out switching, and the connecting clamping ring can be matched with the imaging lens to carry out imaging processing on the biological sample. According to the two-photon fluorescent multicolor imager, the imaging lenses of different models are changed along with switching of the fixed shell, the imaging lenses can be matched with the two-photon fluorescent multicolor imager body, and the models of the imaging lenses are changed according to different biological samples, so that the imaging flexibility and efficiency of the different biological samples are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of imaging, in particular to a two-photon fluorescence multi-color imaging device. Background Art

[0002] The fluorescence multicolor imaging device is an advanced microscopy device used for multi-channel fluorescence imaging of biological samples. It combines two-photon excitation microscopy and multi-channel imaging technology to achieve high-resolution three-dimensional imaging of biological samples over a wide range of depths.

[0003] At present, a Chinese utility model with publication number: CN202568208U discloses a multi-spectral fluorescence three-dimensional tomography device, which belongs to the field of molecular imaging technology. The device includes: a fluorescence imaging system for obtaining a multi-spectral fluorescence image from a target living body and a data analysis and processing device; wherein the data of the multi-spectral fluorescence image and the original fluorescence spectra of multiple fluorescent reagents used simultaneously in the target living body are input into the data analysis and processing device, and the data analysis and processing device is used to perform three-dimensional optical tomography computational analysis and processing of multi-spectral fluorescence on the data of the multi-spectral fluorescence image based on the original fluorescence spectrum to reconstruct a three-dimensional fluorescence image. The 3D fluorescence image of the 3D tomography device has good accuracy and authenticity, and the user's operation in the 3D tomography process is simple.

[0004] When performing high-resolution three-dimensional imaging of biological samples, fluorescent multi-color imaging equipment is used, and two-photon fluorescent multi-color imaging devices are one of them. However, existing imaging devices lack a fast switching structure and are therefore unable to quickly image different biological samples, reducing the flexibility of the imaging devices when in use. Utility Model Content

[0005] The utility model provides a two-photon fluorescence multicolor imaging device, aiming to solve the problem that the existing imaging equipment lacks a fast switching structure, and thus cannot quickly image different biological samples, reducing the flexibility of the imaging equipment when in use.

[0006] The utility model is implemented as follows: a two-photon fluorescence multi-color imaging device comprises a placement plate and a switching mechanism, wherein a computer is bolted to the right side of the placement plate, a fixing plate is bolted to the rear side of the placement plate, a two-photon fluorescence multi-color imager body is bolted to the top of the fixing plate, a servo motor is bolted to the front side of the top of the fixing plate, the switching mechanism is bolted to the bottom of the servo motor, and an imaging lens is clamped to the inner side of the switching mechanism;

[0007] The switching mechanism includes a switching lever, a fixed shell and a connecting ring. The switching lever is bolted to the output end at the bottom of the servo motor, the fixed shell is bolted to the surface of the switching lever, the connecting ring is bolted to the top of the fixed shell, and the imaging lens is clamped on the inner side of the connecting ring.

[0008] In order to achieve the effect of limiting the position of the biological sample, as a preferred two-photon fluorescence multi-color imaging device of the utility model, the top of the placement plate is bolted with an auxiliary tray, and the top of the auxiliary tray is engraved with anti-slip patterns.

[0009] In order to increase the structural stability of the two-photon fluorescence multi-color imager body, as a preferred two-photon fluorescence multi-color imaging device of the utility model, the bottom of the two-photon fluorescence multi-color imager body is bolted with a reinforcement shell, and the bottom of the reinforcement shell is bolted to the top of the fixed plate.

[0010] In order to increase the stability of the switching lever, as a preferred two-photon fluorescence multi-color imaging device of the utility model, a stabilizing pad is bolted to the surface of the switching lever, and the bottom of the stabilizing pad is bolted to the top of the fixed shell.

[0011] In order to protect the connecting clamp, as a preferred two-photon fluorescence multi-color imaging device of the utility model, a protective pad is bolted to the surface of the fixed shell, and the surface of the protective pad is engraved with anti-slip patterns.

[0012] In order to increase the connection stability of the imaging lens, as a preferred two-photon fluorescence multi-color imaging device of the utility model, a protective sleeve is bolted to the inner side of the connecting clamp ring, and the inner side of the protective sleeve is clamped to the surface of the imaging lens.

[0013] In order to protect the imaging lens, as a preferred two-photon fluorescence multi-color imaging device of the utility model, a protective plate is clamped on the top of the imaging lens, and the protective plate is designed to be circular.

[0014] In order to increase the connection stability of the imaging lens, as a preferred two-photon fluorescence multi-color imaging device of the utility model, an auxiliary ring is bolted to the top of the imaging lens surface, and the surface of the auxiliary ring is engraved with anti-slip patterns.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] The two-photon fluorescence multi-color imaging device is provided with a placement plate and a switching mechanism. The placement plate can support biological samples. The computer can cooperate with the two-photon fluorescence multi-color imager body to perform imaging processing on the biological samples. The fixing plate can limit the two-photon fluorescence multi-color imager body and the servo motor. The servo motor can cooperate with the switching rotating rod to drive the fixed housing to switch. The connecting clamp can cooperate with the imaging lens. With the switching of the fixed housing, different types of imaging lenses are changed. The imaging lens can cooperate with the two-photon fluorescence multi-color imager body. According to different biological samples, the type of the imaging lens is changed, thereby improving the flexibility and efficiency of imaging different biological samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is the overall structural diagram of the two-photon fluorescence multi-color imaging device of the utility model;

[0018] Figure 2 It is a structural schematic diagram of the auxiliary tray in the utility model;

[0019] Figure 3 It is a structural schematic diagram of the reinforced shell in the utility model;

[0020] Figure 4 It is a structural schematic diagram of the switching mechanism in the utility model;

[0021] Figure 5 It is a structural schematic diagram of the auxiliary ring in the utility model.

[0022] In the figure, 1. placement plate; 2. computer; 3. fixing plate; 4. two-photon fluorescence multi-color imager body; 5. servo motor; 6. switching mechanism; 601. switching lever; 602. fixed shell; 603. connecting clamp; 7. imaging lens; 8. auxiliary tray; 9. reinforcement shell; 10. stabilizing pad; 11. protective pad; 12. protective cover; 13. protective plate; 14. auxiliary ring. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.

[0024] In the description of the present invention, it should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, in the description of the present invention, "multiple" means two or more, unless otherwise clearly and specifically defined.

[0025] See also Figure 1-5 The utility model provides a technical solution: a two-photon fluorescence multi-color imaging device, comprising a placement plate 1 and a switching mechanism 6, a computer 2 is bolted to the right side of the placement plate 1, a fixing plate 3 is bolted to the rear side of the placement plate 1, a two-photon fluorescence multi-color imager body 4 is bolted to the top of the fixing plate 3, a servo motor 5 is bolted to the front side of the top of the fixing plate 3, the switching mechanism 6 is bolted to the bottom of the servo motor 5, and an imaging lens 7 is clamped on the inner side of the switching mechanism 6;

[0026] The switching mechanism 6 includes a switching lever 601, a fixed housing 602 and a connecting ring 603. The switching lever 601 is bolted to the output end at the bottom of the servo motor 5, the fixed housing 602 is bolted to the surface of the switching lever 601, the connecting ring 603 is bolted to the top of the fixed housing 602, and the imaging lens 7 is clamped on the inner side of the connecting ring 603.

[0027] In this embodiment: by setting a placement plate 1 and a switching mechanism 6, the placement plate 1 can support the biological sample, the computer 2 can cooperate with the two-photon fluorescence multi-color imager body 4 to perform imaging processing on the biological sample, the fixed plate 3 can limit the two-photon fluorescence multi-color imager body 4 and the servo motor 5, the servo motor 5 can cooperate with the switching lever 601 to drive the fixed shell 602 to switch, the connecting clamp ring 603 can cooperate with the imaging lens 7, and with the switching of the fixed shell 602, different models of imaging lenses 7 are changed, and the imaging lens 7 can cooperate with the two-photon fluorescence multi-color imager body 4. According to different biological samples, the model of the imaging lens 7 is changed, thereby improving the flexibility and efficiency of imaging different biological samples.

[0028] As a technical optimization solution of the present invention, an auxiliary tray 8 is bolted to the top of the placement plate 1, and the top of the auxiliary tray 8 is engraved with anti-slip patterns.

[0029] In this embodiment, the auxiliary tray 8 is provided to cooperate with the placement plate 1 to further limit the position of the biological sample and increase the stability of the biological sample during imaging.

[0030] As a technical optimization solution of the utility model, a reinforcement shell 9 is bolted to the bottom of the two-photon fluorescence multi-color imager body 4 , and the bottom of the reinforcement shell 9 is bolted to the top of the fixing plate 3 .

[0031] In this embodiment: by setting a reinforcement shell 9, it can cooperate with the two-photon fluorescence multi-color imager body 4 to further reinforce the connection between the two-photon fluorescence multi-color imager body 4 and the fixed plate 3, thereby further increasing the structural stability when the two-photon fluorescence multi-color imager body 4 and the fixed plate 3 are connected.

[0032] As a technical optimization solution of the present invention, a stabilizing pad 10 is bolted to the surface of the switching lever 601 , and the bottom of the stabilizing pad 10 is bolted to the top of the fixed housing 602 .

[0033] In this embodiment, by providing a stabilizing pad 10 , it can cooperate with the switching lever 601 to further increase the stability when the switching lever 601 is connected to the fixed housing 602 .

[0034] As a technical optimization solution of the present invention, a protective pad 11 is bolted to the surface of the fixed shell 602, and the surface of the protective pad 11 is engraved with anti-slip patterns.

[0035] In this embodiment, the protective pad 11 is provided to cooperate with the fixed shell 602 , thereby further increasing the stability of the contact between the fixed shells 602 and the fixed shells 602 .

[0036] As a technical optimization solution of the present invention, a protective sleeve 12 is bolted to the inner side of the connecting clamp ring 603 , and the inner side of the protective sleeve 12 is clamped to the surface of the imaging lens 7 .

[0037] In this embodiment, the protective cover 12 is provided to cooperate with the imaging lens 7 , thereby further increasing the stability of the imaging lens 7 when connected to the connecting clamp 603 .

[0038] As a technical optimization solution of the present invention, a protective plate 13 is clamped on the top of the imaging lens 7, and the protective plate 13 is designed to be circular.

[0039] In this embodiment: by setting a protective plate 13, the protective plate 13 is a protective structure made of glass material, which can cooperate with the imaging lens 7 to protect the top of the imaging lens 7. The circular design can better fit the shape of the imaging lens 7, so that it can better protect the top of the imaging lens 7.

[0040] As a technical optimization solution of the present invention, an auxiliary ring 14 is bolted to the top of the surface of the imaging lens 7, and the surface of the auxiliary ring 14 is engraved with anti-slip patterns.

[0041] In this embodiment, the auxiliary ring 14 is provided to cooperate with the imaging lens 7 , thereby further increasing the structural stability of the imaging lens 7 when it is clamped in the connecting clamp ring 603 .

[0042] Working principle: First, place the biological sample to be imaged on the placement plate 1, then install the required type of imaging lens 7 in the connecting ring 603, then energize and start the servo motor 5, the servo motor 5 will drive the switching lever 601 to rotate the fixed shell 602, and the fixed shell 602 will drive the connecting ring 603 to move to the two-photon fluorescence multi-color imager body 4, then energize and start the two-photon fluorescence multi-color imager body 4, the two-photon fluorescence multi-color imager body 4 will image the biological sample through the imaging lens 7, and transmit the imaged information to the computer 2, and the computer 2 will display the imaged information in the form of an image.

[0043] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A two-photon fluorescence multicolor imaging device, comprising a placement plate (1) and a switching mechanism (6), characterized in that: The right side of the placement plate (1) is bolted to a computer (2), the rear side of the placement plate (1) is bolted to a fixing plate (3), the top of the fixing plate (3) is bolted to a two-photon fluorescence multicolor imager body (4), the front side of the top of the fixing plate (3) is bolted to a servo motor (5), the switching mechanism (6) is bolted to the bottom of the servo motor (5), and the inner side of the switching mechanism (6) is clamped with an imaging lens (7); The switching mechanism (6) comprises a switching lever (601), a fixed housing (602) and a connecting ring (603); the switching lever (601) is bolted to the output end at the bottom of the servo motor (5); the fixed housing (602) is bolted to the surface of the switching lever (601); the connecting ring (603) is bolted to the top of the fixed housing (602); and the imaging lens (7) is clamped to the inner side of the connecting ring (603).

2. A two-photon fluorescence multicolor imaging device according to claim 1, characterized in that: An auxiliary tray (8) is bolted to the top of the placement plate (1), and anti-slip patterns are engraved on the top of the auxiliary tray (8).

3. A two-photon fluorescence multicolor imaging device according to claim 1, characterized in that: A reinforcement shell (9) is bolted to the bottom of the two-photon fluorescence multi-color imager body (4), and the bottom of the reinforcement shell (9) is bolted to the top of the fixing plate (3).

4. A two-photon fluorescence multicolor imaging device according to claim 1, characterized in that: A stabilizing pad (10) is bolted to the surface of the switching lever (601), and the bottom of the stabilizing pad (10) is bolted to the top of the fixed housing (602).

5. A two-photon fluorescence multicolor imaging device according to claim 1, characterized in that: A protective pad (11) is bolted to the surface of the fixed shell (602), and the surface of the protective pad (11) is engraved with anti-slip patterns.

6. A two-photon fluorescence multicolor imaging device according to claim 1, characterized in that: A protective sleeve (12) is bolted to the inner side of the connecting clamp ring (603), and the inner side of the protective sleeve (12) is clamped to the surface of the imaging lens (7).

7. A two-photon fluorescence multicolor imaging device according to claim 1, characterized in that: A protective plate (13) is clamped on the top of the imaging lens (7), and the protective plate (13) is circular in shape.

8. A two-photon fluorescence multicolor imaging device according to claim 1, characterized in that: An auxiliary ring (14) is bolted to the top of the surface of the imaging lens (7), and the surface of the auxiliary ring (14) is engraved with anti-slip patterns.

Images