Biological enzyme purification and separation device

Abstract

The utility model provides a biological enzyme purification and separation device, which relates to the technical field of biological enzyme purification and separation and comprises an outer cylinder and an inner cylinder, the inner cylinder is arranged inside the outer cylinder, a fixed circular truncated cone is arranged inside the inner cylinder, a separation cylinder is arranged above the fixed circular truncated cone, a cooling cylinder is arranged inside the separation cylinder, and a sealing cover component is arranged above the outer cylinder. The sealing cover assembly is used for blocking the tops of the outer cylinder and the separation cylinder, a supporting table is arranged at the top of the cooling cylinder, a cooling liquid circulating assembly is arranged at the top of the supporting table, and a stirring mechanism is arranged in the cooling cylinder; the cooling cylinder is arranged in the separation cylinder, so that biological enzyme in the separation cylinder can be efficiently cooled by cooling liquid in the cooling cylinder, and the cooling liquid can maintain a stable cooling effect under the action of the cooling liquid circulating assembly in the cooling process; a stirring mechanism is arranged in a matched manner, so that the biological enzyme in the separation barrel can be uniformly cooled by cooling liquid.

Description

Technical Field

[0001] The utility model relates to the technical field of biological enzyme purification and separation, in particular to a biological enzyme purification and separation device. Background Art

[0002] The purification and separation of biological enzymes refers to the process of treating the enzyme-containing raw materials after cell crushing with appropriate solvents under certain conditions to fully dissolve the enzymes into the extract. The biological enzyme solution often needs to be cooled for a period of time before purification and separation to obtain a better centrifugal effect, but most of the current cooling equipment is separated from the centrifugal equipment, which affects the separation effect.

[0003] For example, Chinese patent CN 211947013 U discloses a biological enzyme purification and separation device, including a cooling tank, a cooling jacket is installed in the lower part of the cooling tank, a first delivery pipe is installed at the bottom of the cooling tank, a first delivery pump is installed on the first delivery pipe, one end of the first delivery pipe is connected to the bottom of the cooling tank, and the other end is connected to the upper part of the centrifugal tank, a second delivery pipe is installed at the bottom of the centrifugal tank, a second delivery pump is installed on the second delivery pipe, one end of the second delivery pipe is connected to the bottom of the centrifugal tank, and the other end is connected to the upper part of the filter tank, and a filter screen is installed inside the centrifugal tank. The utility model improves the effect of purification and separation, reduces the pollution during purification and separation, can meet the needs of the existing market, and can produce better economic benefits. In the above patent, the separation tank and the cooling tank are separately arranged, the biological enzyme is first cooled in the cooling tank and then transported to the separation tank, and no cooling treatment is performed during the separation process, resulting in the biological enzyme cooling effect starting to decline after being transported out of the cooling tank. Therefore, the utility model proposes a biological enzyme purification and separation device to solve the shortcomings in the prior art. Utility Model Content

[0004] In view of the above problems, the purpose of the utility model is to provide a biological enzyme purification and separation device. By arranging a cooling cylinder inside the separation cylinder, the biological enzyme inside the separation cylinder can be efficiently cooled by utilizing the coolant in the cooling cylinder. During the cooling process, the coolant can maintain a stable cooling effect under the action of the coolant circulation component. The stirring mechanism arranged in conjunction can enable the coolant to evenly cool the biological enzyme inside the separation cylinder, thereby ensuring that the separation efficiency and quality of the biological enzyme can be improved when performing separation operations in the separation cylinder.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A biological enzyme purification and separation device comprises an outer cylinder and an inner cylinder, wherein the inner cylinder is arranged inside the outer cylinder, a separation motor is provided at the lower part of the outer cylinder, an output end of the separation motor is connected to the bottom of the inner cylinder, a fixed truncated cone is provided inside the inner cylinder, a plurality of fixed truncated cones are provided, a separation cylinder is provided above the fixed truncated cone, a cooling cylinder is provided inside the separation cylinder, a sealing cover assembly is provided above the outer cylinder, the sealing cover assembly is used to seal the outer cylinder and the top of the separation cylinder, a support platform is provided at the top of the cooling cylinder, a coolant circulation assembly is provided at the top of the support platform, the coolant circulation assembly is connected to the interior of the cooling cylinder, a stirring mechanism is provided inside the cooling cylinder, the stirring mechanism is driven by a stirring motor, and the top of the stirring motor is connected to the bottom of the support platform.

[0007] A further improvement is that: a hole groove is provided on the side wall of the separation cylinder, and a plurality of the hole grooves are provided, and the plurality of the hole grooves are all inclinedly arranged.

[0008] A further improvement is that the coolant circulation component includes a shell, a radiator, a circulation pump, a liquid inlet pipe and a liquid outlet pipe. The support platform is provided with a shell on the top, and a circulation pump and a radiator are provided inside the shell. The inlet end of the circulation pump is connected to the liquid inlet pipe, the outlet end of the circulation pump is connected to the liquid inlet of the radiator, and the liquid outlet of the radiator is connected to the liquid outlet pipe.

[0009] A further improvement is that a pipe groove is provided inside the side wall of the cooling cylinder, the lower end of the liquid inlet pipe extends to the bottom of the pipe groove, and the lower end of the liquid inlet pipe is connected to the bottom of the cooling cylinder, and the lower end of the liquid outlet pipe passes through the support platform and leads to the top of the cooling cylinder.

[0010] A further improvement is that the stirring mechanism includes a stirring rod and stirring blades, a protective cover is provided above the interior of the cooling cylinder, the upper end of the stirring rod passes through the protective cover and is connected to the output end of the stirring motor, the stirring blades are arranged on the stirring rod, and the stirring blades are symmetrically arranged in multiple groups on the stirring rod.

[0011] A further improvement is that: an installation cavity is provided inside the inner cylinder, the top of the installation cavity is a hollow structure, a guide hopper is provided in the installation cavity, a discharge pipe is provided at the bottom of the guide hopper, and a valve is provided on the discharge pipe.

[0012] A further improvement is that: the inner cylinder is provided with an opening, the opening is adapted to the setting position of the discharge pipe, and the outer cylinder is provided with a door body, the door body is adapted to the setting position of the opening.

[0013] Further improvement is that the sealing cover assembly includes an outer cylinder cover and a separation cylinder block, the outer cylinder cover is provided above the outer cylinder, and a separation cylinder block is provided inside the outer cylinder cover, the setting position and number of the separation cylinder block are adapted to the position and number of the cooling cylinder, the top of the separation cylinder block is rotatably connected to the top of the inner part of the outer cylinder cover, and the top of the outer cylinder cover is provided with a lifting ear.

[0014] The beneficial effects of the utility model are as follows: the utility model can realize efficient cooling of the biological enzyme inside the separation cylinder by using the coolant in the cooling cylinder by arranging the cooling cylinder inside the separation cylinder; during the cooling process, the coolant can maintain a stable cooling effect under the action of the coolant circulation component; the stirring mechanism arranged in cooperation can make the coolant evenly cool the biological enzyme inside the separation cylinder, thereby ensuring that the separation efficiency and quality of the biological enzyme can be improved when performing separation operations in the separation cylinder;

[0015] The utility model has multiple separation cylinders arranged inside the inner cylinder, and the multiple separation cylinders can divide a quantitative biological enzyme into multiple single portions for synchronous separation. Since the amount of the single biological enzyme is small, not only the separation efficiency can be improved, but also the cooling effect can be improved when the cooling cylinder cools it. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the main structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the installation structure of the coolant circulation component of the utility model;

[0018] Figure 3 This is a schematic diagram of the main structure of the coolant circulation component of the utility model;

[0019] Figure 4 It is a schematic diagram of the disassembly of the outer cylinder and inner cylinder installation structure of the utility model.

[0020] Among them: 1. outer cylinder; 2. inner cylinder; 3. separation motor; 4. fixed truncated table; 5. separation cylinder; 6. cooling cylinder; 7. sealing cover assembly; 701. outer cylinder cover; 702. separation cylinder block; 8. support platform; 9. coolant circulation assembly; 901. outer shell; 902. radiator; 903. circulation pump; 904. liquid inlet pipe; 905. liquid outlet pipe; 10. stirring mechanism; 1001. stirring rod; 1002. stirring blade; 1003. protective cover; 11. stirring motor; 12. hole groove; 13. installation cavity; 14. guide bucket; 15. discharge pipe; 16. valve; 17. opening; 18. door body. DETAILED DESCRIPTION

[0021] In order to deepen the understanding of the present invention, the present invention will be further described in detail below in conjunction with an embodiment. The embodiment is only used to explain the present invention and does not constitute a limitation on the protection scope of the present invention. Example

[0022] according to Figure 1-3As shown, this embodiment proposes a bio-enzyme purification and separation device, including an outer cylinder 1 and an inner cylinder 2, wherein the inner cylinder 2 is arranged inside the outer cylinder 1, and a separation motor 3 is provided at the lower part of the outer cylinder 1, and the output end of the separation motor 3 is connected to the bottom of the inner cylinder 2, and a fixed truncated cone 4 is provided inside the inner cylinder 2, and the fixed truncated cone 4 is provided in multiple groups, and a separation cylinder 5 is provided above the fixed truncated cone 4, and a cooling cylinder 6 is provided inside the separation cylinder 5, and a sealing cover assembly 7 is provided above the outer cylinder 1, and the sealing cover assembly 7 is used to seal the outer cylinder 1 and the top of the separation cylinder 5, and a support platform 8 is provided on the top of the cooling cylinder 6, and a coolant circulation assembly 9 is provided on the top of the support platform 8, and the coolant circulation assembly 9 is connected to the inside of the cooling cylinder 6, and a stirring mechanism 10 is provided in the cooling cylinder 6, and the stirring mechanism 10 is driven by a stirring motor 11, and the top of the stirring motor 11 is connected to the bottom of the support platform 8.

[0023] When the bio-enzyme purification and separation device of the utility model is separated, when the bio-enzyme is transported from the external cooling tank to the separation cylinder, the coolant in the cooling cylinder continues to cool the bio-enzyme in the separation cylinder, and then the separation motor is started to drive the inner cylinder 2 to rotate. The inner cylinder 2 rotates so that multiple separation cylinders 5 can perform separation operations. During the process, the coolant in the cooling cylinder 6 continuously cools the separation cylinder. In order to ensure the cooling effect of the coolant, the coolant circulation component 99 and the stirring mechanism 10 continuously process the coolant to ensure the best cooling state.

[0024] The side wall of the separation cylinder 5 is provided with a hole groove 12, and the hole groove 12 is provided with a plurality of holes, and the plurality of hole grooves 12 are all inclined. The inclined hole groove 12 can facilitate the biological enzyme inside the separation cylinder 5 to separate out of the separation cylinder 5 and enter the inner cylinder 2.

[0025] The cooling liquid circulation assembly 9 includes a shell 901, a cold row 902, a circulation pump 903, a liquid inlet pipe 904 and a liquid outlet pipe 905. The top of the support platform 8 is provided with a shell 901, and the circulation pump 903 and the cold row 902 are provided inside the shell 901. The inlet end of the circulation pump 903 is connected to the liquid inlet pipe 904, and the outlet end of the circulation pump 903 is connected to the liquid inlet of the cold row 902. The liquid outlet of the cold row 902 is connected to the liquid outlet pipe 905. A pipe groove is provided inside the side wall of the cooling cylinder 6, and the lower end of the liquid inlet pipe 904 extends to the lower part of the inside of the pipe groove, and the lower end of the liquid inlet pipe 904 is connected to the lower part of the inside of the cooling cylinder 6, and the lower end of the liquid outlet pipe 905 passes through the support platform 8 and leads to the upper part of the inside of the cooling cylinder 6. When the coolant circulation assembly 9 is running, the circulation pump 903 and the liquid inlet pipe 904 cooperate to suck the coolant from the bottom of the cooling cylinder 6 into the cooling row 902 for cooling, and then circulate it again to the inside of the cooling cylinder 6 through the cooling row 902 and the liquid outlet pipe 905.

[0026] The stirring mechanism 10 includes a stirring rod 1001 and stirring blades 1002. A protective cover 1003 is provided above the interior of the cooling cylinder 6. The upper end of the stirring rod 1001 passes through the protective cover 1003 and is connected to the output end of the stirring motor 11. The stirring blades 1002 are provided on the stirring rod 1001. Multiple groups of stirring blades 1002 are symmetrically provided on the stirring rod 1001. When the coolant cools the biological enzyme inside the separation cylinder 5, the stirring motor 11 is started to drive the stirring rod 1001 and the stirring blades 1002 to stir the coolant, so that the coolant fully cools the separation cylinder 5.

[0027] The sealing cover assembly 7 includes an outer cylinder cover 701 and a separation cylinder block 702. The outer cylinder cover 701 is provided above the outer cylinder 1, and the separation cylinder block 702 is provided inside the outer cylinder cover 701. The setting position and number of the separation cylinder block 702 are adapted to the position and number of the cooling cylinder 6. The top of the separation cylinder block 702 is rotatably connected to the upper inside of the outer cylinder cover 701, and the top of the outer cylinder cover 701 is provided with a lifting ear. In the utility model, in order to prevent the biological enzyme from splashing from the top of the separation cylinder 5 during the separation process, the outer cylinder 1 and the top of the separation cylinder 5 are sealed by controlling the sealing cover assembly 7 to descend. During the process, the outer cylinder cover 701 seals the outer cylinder 1, and the separation cylinder block 702 blocks the top of the separation cylinder 5. Since the separation cylinder 5 needs to rotate, the separation cylinder block 702 that is rotatably set at this time ensures that the rotation process of the separation cylinder 5 is not interfered. Example

[0028] according to Figure 1-4 As shown, this embodiment proposes a bio-enzyme purification and separation device, including an outer cylinder 1 and an inner cylinder 2, wherein the inner cylinder 2 is arranged inside the outer cylinder 1, and a separation motor 3 is provided at the lower part of the outer cylinder 1, and the output end of the separation motor 3 is connected to the bottom of the inner cylinder 2, and a fixed truncated cone 4 is provided inside the inner cylinder 2, and the fixed truncated cone 4 is provided in multiple groups, and a separation cylinder 5 is provided above the fixed truncated cone 4, and a cooling cylinder 6 is provided inside the separation cylinder 5, and a sealing cover assembly 7 is provided above the outer cylinder 1, and the sealing cover assembly 7 is used to seal the outer cylinder 1 and the top of the separation cylinder 5, and a support platform 8 is provided on the top of the cooling cylinder 6, and a coolant circulation assembly 9 is provided on the top of the support platform 8, and the coolant circulation assembly 9 is connected to the inside of the cooling cylinder 6, and a stirring mechanism 10 is provided in the cooling cylinder 6, and the stirring mechanism 10 is driven by a stirring motor 11, and the top of the stirring motor 11 is connected to the bottom of the support platform 8.

[0029] The inner cylinder 2 is provided with an installation cavity 13, the top of the installation cavity 13 is a hollow structure, a guide hopper 14 is provided in the installation cavity 13, a discharge pipe 15 is provided at the bottom of the guide hopper 14, and a valve 16 is provided on the discharge pipe 15. The inner cylinder 2 is provided with an opening 17, the opening 17 is adapted to the setting position of the discharge pipe 15, and the outer cylinder 1 is provided with a door body 18, the door body 18 is adapted to the setting position of the opening 17. When the separated biological enzyme enters the inner cylinder 2, it will enter the inside of the guide hopper 14 through the hollow structure at the top of the installation cavity 13. When the biological enzyme needs to be collected, the door body 18 is opened and the valve on the discharge pipe 15 is opened from the opening 17 to discharge the material.

[0030] The utility model can realize efficient cooling of the biological enzyme inside the separation cylinder 5 by using the coolant in the cooling cylinder 6 by arranging the cooling cylinder 6 inside the separation cylinder 5. During the cooling process, the coolant can maintain a stable cooling effect under the action of the coolant circulation component 9. The stirring mechanism 10 arranged in cooperation can make the coolant evenly cool the biological enzyme inside the separation cylinder 5, thereby ensuring that the separation efficiency and quality of the biological enzyme can be improved when the separation operation is carried out in the separation cylinder. The utility model has a plurality of separation cylinders 5 arranged inside the inner cylinder, and the plurality of separation cylinders 5 can divide a certain amount of biological enzyme into a plurality of single portions for synchronous separation. Since the amount of a single portion of biological enzyme is small, not only the separation efficiency can be improved, but also the cooling effect can be improved when the cooling cylinder 6 cools it.

[0031] The above shows and describes the basic principles, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A biological enzyme purification and separation device, characterized in that: The invention comprises an outer cylinder (1) and an inner cylinder (2), wherein the inner cylinder (2) is arranged inside the outer cylinder (1), a separation motor (3) is arranged at the lower part inside the outer cylinder (1), and the output end of the separation motor (3) is connected to the bottom of the inner cylinder (2), a fixed truncated table (4) is arranged inside the inner cylinder (2), and the fixed truncated table (4) is provided with a plurality of groups, a separation cylinder (5) is arranged above the fixed truncated table (4), and a cooling cylinder (6) is arranged inside the separation cylinder (5), a sealing cover assembly (7) is arranged above the outer cylinder (1), and the sealing cover assembly (7) is used to seal the top of the outer cylinder (1) and the separation cylinder (5), a support platform (8) is arranged at the top of the cooling cylinder (6), and a coolant circulation assembly (9) is arranged at the top of the support platform (8), and the coolant circulation assembly (9) is connected to the inside of the cooling cylinder (6), and a stirring mechanism (10) is arranged inside the cooling cylinder (6), and the stirring mechanism (10) is driven by a stirring motor (11), and the top of the stirring motor (11) is connected to the bottom of the support platform (8).

2. A biological enzyme purification and separation device according to claim 1, characterized in that: A hole groove (12) is provided on the side wall of the separation cylinder (5), a plurality of the hole grooves (12) are provided, and the plurality of the hole grooves (12) are all arranged obliquely.

3. A biological enzyme purification and separation device according to claim 1, characterized in that: The coolant circulation component (9) comprises a shell (901), a cold row (902), a circulation pump (903), a liquid inlet pipe (904) and a liquid outlet pipe (905); the shell (901) is provided on the top of the support platform (8); the circulation pump (903) and the cold row (902) are provided inside the shell (901); the inlet end of the circulation pump (903) is connected to the liquid inlet pipe (904); the outlet end of the circulation pump (903) is connected to the liquid inlet of the cold row (902); and the liquid outlet of the cold row (902) is connected to the liquid outlet pipe (905).

4. A biological enzyme purification and separation device according to claim 3, characterized in that: A pipe groove is provided inside the side wall of the cooling cylinder (6), the lower end of the liquid inlet pipe (904) extends to the lower part of the pipe groove, and the lower end of the liquid inlet pipe (904) is connected to the lower part of the cooling cylinder (6), and the lower end of the liquid outlet pipe (905) passes through the support platform (8) and leads to the upper part of the cooling cylinder (6).

5. A biological enzyme purification and separation device according to claim 1, characterized in that: The stirring mechanism (10) comprises a stirring rod (1001) and stirring blades (1002); a protective cover (1003) is provided above the interior of the cooling cylinder (6); the upper end of the stirring rod (1001) passes through the protective cover (1003) and is connected to the output end of the stirring motor (11); the stirring blades (1002) are arranged on the stirring rod (1001); and a plurality of stirring blades (1002) are symmetrically provided on the stirring rod (1001).

6. A biological enzyme purification and separation device according to claim 1, characterized in that: An installation cavity (13) is provided inside the inner cylinder (2); the top of the installation cavity (13) is a hollow structure; a flow guide hopper (14) is provided inside the installation cavity (13); a discharge pipe (15) is provided at the bottom of the flow guide hopper (14); and a valve (16) is provided on the discharge pipe (15).

7. A biological enzyme purification and separation device according to claim 1, characterized in that: The inner cylinder (2) is provided with an opening (17), and the opening (17) is adapted to the setting position of the discharge pipe (15); the outer cylinder (1) is provided with a door body (18), and the door body (18) is adapted to the setting position of the opening (17).

8. The bio-enzyme purification and separation device according to claim 1, characterized in that: The sealing cover assembly (7) comprises an outer cylinder cover (701) and a separation cylinder block (702); the outer cylinder cover (701) is provided on the top of the outer cylinder (1); the separation cylinder block (702) is provided inside the outer cylinder cover (701); the setting position and quantity of the separation cylinder block (702) are adapted to the position and quantity of the cooling cylinder (6); the top of the separation cylinder block (702) is rotatably connected to the top of the inner part of the outer cylinder cover (701); and the top of the outer cylinder cover (701) is provided with a lifting ear.

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