Temperature control unit and system applied to sample to be detected of SPR (Surface Plasmon Resonance) detector

Abstract

The utility model provides a temperature control unit and system applied to a to-be-detected sample of an SPR detector, the temperature control unit is used for being placed in an SPR detection bin, the temperature control unit comprises a heat preservation box shell, a heat preservation box lining, a to-be-detected sample support and a heat preservation box cover, the heat preservation box shell is provided with a sealing strip groove, a sealing strip is arranged in the sealing strip groove, and the to-be-detected sample is placed in the heat preservation box lining. A motor driving device is arranged at the rear end of the heat preservation box shell; the heat preservation box lining is nested in the heat preservation box shell; the to-be-tested sample bracket is placed in the heat preservation box lining; a sealing strip pressing edge is arranged on the heat preservation box cover and used for pressing the sealing strip, the heat preservation box cover is connected with the heat preservation box shell through a motor driving device, and the motor driving device drives the heat preservation box cover to turn over so that opening and closing of the heat preservation box cover can be achieved. The temperature control device improves the temperature control precision of the to-be-detected sample of the SPR detector, has the characteristics of low power consumption, high efficiency, low sealing requirement and the like, and enables the temperature control of the to-be-detected sample to be simple and easy to realize.

Description

Technical Field

[0001] The utility model relates to the technical field of surface plasmon resonance detection, in particular to a temperature control unit and a system applied to a sample to be detected by an SPR detector. Background Art

[0002] Surface Plasmon Resonance (SPR) is a new biosensing analysis technology based on the SPR principle. This technology monitors the changes in each step of the molecular binding process in real time through the binding and dissociation curves of molecular complexes occurring on the sensor surface.

[0003] The general use scenario of surface plasmon resonance detectors is to test the interaction of biomolecules, such as affinity testing, activity testing, activity concentration testing, biomolecule screening and molecular fishing, etc. Based on the use scenario of the instrument and the characteristics of SPR technology itself, temperature control plays a very important role in surface plasmon resonance detection.

[0004] The activity of biological molecules is related to temperature. In a low temperature environment, their activity will decrease, but the activity retention time will be greatly increased. In a high temperature environment, the molecules will be inactivated. Therefore, if the temperature of the sample storage part of the instrument is lowered to below 10°C, the storage time of the molecules will be increased, and the instrument can test more samples.

[0005] At present, the SPR detector does not control the temperature of the sample to be tested. The sample to be tested is directly tested after being taken out of the incubator. However, as time goes by, the sample to be tested has no insulation and temperature control device, and the temperature will gradually approach room temperature. The sample to be tested will gradually lose its activity, resulting in detection failure or detection error, which will have serious consequences for subsequent experiments. Utility Model Content

[0006] The utility model aims to provide a temperature control unit and system for samples to be tested in an SPR detector, which improves the thermal insulation effect and temperature control accuracy of the samples to be tested in the SPR detector, has the characteristics of low power consumption, high efficiency, and low sealing requirements, so that the temperature control of the samples to be tested becomes simple and easy to achieve.

[0007] To achieve the above purpose, the utility model provides the following solutions:

[0008] The utility model provides a temperature control unit for a sample to be tested in an SPR detector, the temperature control unit is used to be placed in an SPR detection chamber, and the temperature control unit comprises: an insulation box shell, an insulation box lining, a sample holder to be tested, and an insulation box cover.

[0009] The heat preservation box shell is provided with a sealing strip groove, a sealing strip is arranged in the sealing strip groove, and a motor driving device is arranged at the rear end of the heat preservation box shell;

[0010] The heat preservation box liner is nested in the heat preservation box shell;

[0011] The sample holder to be tested is placed in the lining of the thermal insulation box;

[0012] The insulation box cover is provided with a sealing strip pressure edge, and the sealing strip pressure edge is used to press the sealing strip. The insulation box cover is connected to the insulation box shell through the motor drive device, and the motor drive device drives the insulation box cover to flip and realize the opening and closing of the insulation box cover.

[0013] Furthermore, the sample holder to be tested includes a holder body, a boss, and a positioning hole. A plurality of the bosses are arranged in multiple rows on the upper surface of the holder body. The positioning hole is opened between two adjacent bosses arranged in the same row. The test tube containing the sample to be tested is placed in the positioning hole, and the top edge of the test tube is clamped on the boss.

[0014] Furthermore, the front and rear sides of the bracket body are correspondingly provided with embedded grooves.

[0015] Furthermore, an outward protrusion is provided at a position of the sealing strip groove corresponding to the embedded groove.

[0016] Furthermore, the position of the sealing strip pressed edge corresponds to the position of the sealing strip groove, and the sealing strip pressed edge and the sealing strip groove have the same shape.

[0017] Furthermore, the motor drive device includes a drive motor and a transmission mechanism, the drive motor is transmission-connected to the transmission mechanism, and the transmission mechanism is transmission-connected to the heat-insulating box cover.

[0018] Furthermore, the lining of the heat preservation box and the holder for the sample to be tested are made of a material with high thermal conductivity, and the material with high thermal conductivity is gold, copper, aluminum or graphene.

[0019] The utility model also provides a temperature control system applied to a sample to be tested by an SPR detector, comprising the temperature control unit applied to the sample to be tested by the SPR detector, and also comprising an SPR detection chamber, a primary temperature control device, a secondary temperature control device, and a central controller;

[0020] The primary temperature control device is arranged in the SPR detection chamber, and includes a heat conduction platform, a first temperature regulator and a first temperature sensor. The temperature control unit is fixedly placed on the heat conduction platform. The first temperature regulator is arranged below the heat conduction platform and is used to regulate the temperature of the heat conduction platform. The heat conduction platform sequentially transmits the temperature to the sample to be tested through the lining of the heat preservation box and the sample holder to be tested. The first temperature sensor is arranged at the bottom of the temperature control unit and is used to collect the temperature of the temperature control unit.

[0021] The secondary temperature control device includes a second temperature controller and a second temperature sensor, wherein the second temperature controller is arranged behind the chamber body of the SPR detection chamber and is used to control the ambient temperature in the SPR detection chamber, and the second temperature sensor is arranged in the SPR detection chamber and is used to collect the ambient temperature in the SPR detection chamber;

[0022] The first temperature controller, the first temperature sensor, the second temperature controller and the second temperature sensor are electrically connected to the central controller respectively.

[0023] Furthermore, the first temperature controller and the second temperature controller are refrigerators.

[0024] According to the specific embodiment provided by the utility model, the utility model discloses the following technical effects: the temperature control unit and system applied to the sample to be tested of the SPR detector provided by the utility model, the temperature control unit is used to be placed in the SPR detection chamber, and the double-layer insulation space of the temperature control unit and the SPR detection chamber is realized; the temperature control unit has a simple structure and is easy to use, and is suitable for the SPR detector. The SPR detector does not need to be structurally improved. As long as the temperature control unit is added, the insulation effect in a smaller space can be achieved for the sample to be tested, which is conducive to precise temperature control, and combined with the original temperature control device to achieve a secondary temperature control effect. The utility model is composed of a two-level temperature control system, and each level of temperature control is independently controlled and affects each other, effectively controlling the temperature of the sample to be tested within the required range, and the temperature can be maintained for a long time. This utility model uses a smaller power to achieve a greater effect through this superposition method. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0026] Figure 1The structural intention of the temperature control unit provided by the utility model for the sample to be tested in the SPR detector;

[0027] Figure 2 A schematic diagram of the structure of the heat preservation box shell provided by the utility model;

[0028] Figure 3 A schematic diagram of the structure of the sample support to be tested provided by the utility model;

[0029] Figure 4 A schematic diagram of the structure of the heat preservation box cover provided by the utility model;

[0030] Figure 5 A schematic diagram of the structure of the motor drive device provided by the utility model;

[0031] Figure 6 A schematic diagram of the structure of a temperature control system for a sample to be tested in an SPR detector provided by the utility model;

[0032] Figure 7 A schematic diagram of the structure of the primary temperature control device provided by the utility model;

[0033] Figure 8 A schematic diagram of the installation structure of the secondary temperature control device provided by the utility model;

[0034] Fig. 9 A control principle diagram of a temperature control system for a sample to be tested in an SPR detector provided in an embodiment of the utility model;

[0035] Fig.10 A schematic diagram of a control embodiment of a temperature control system for a sample to be tested in an SPR detector provided by an embodiment of the utility model;

[0036] Explanation of the reference numerals in the accompanying drawings: 1. insulation box shell; 11. sealing strip groove; 12. protrusion; 2. insulation box lining; 3. sample holder to be tested; 31. holder body; 32. boss; 33. positioning hole; 34. embedded groove; 4. insulation box cover; 41. sealing strip edge pressing; 5. motor drive device; 51. drive motor; 52. transmission mechanism; 6. sealing strip; 7. temperature control unit; 8. primary temperature control device; 81. first temperature regulator; 82. heat conduction platform; 83. first temperature sensor; 9. secondary temperature control device; 91. second temperature regulator; 92. second temperature sensor; 10. SPR detection chamber; 101. chamber door. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0038] The utility model aims to provide a temperature control unit and system for samples to be tested in an SPR detector, which improves the thermal insulation effect and temperature control accuracy of the samples to be tested in the SPR detector, has the characteristics of low power consumption, high efficiency, and low sealing requirements, so that the temperature control of the samples to be tested becomes simple and easy to achieve.

[0039] In order to make the above-mentioned purposes, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0040] like Figure 1-Figure 5 As shown, the temperature control unit for the sample to be tested in the SPR detector provided by the utility model is used to be placed in the SPR detection chamber, and the temperature control unit includes: an insulation box shell 1, an insulation box lining 2, a sample holder 3 and an insulation box cover 4;

[0041] like Figure 2 As shown, the heat preservation box shell 1 is provided with a sealing strip groove 11, a sealing strip 6 is arranged in the sealing strip groove 11, and a motor driving device 5 is arranged at the rear end of the heat preservation box shell 1;

[0042] The heat preservation box lining 2 is nested in the heat preservation box shell 1; the heat preservation box lining 2 is made of a material with high thermal conductivity such as gold, copper, aluminum, graphene, etc.;

[0043] The sample holder 3 to be tested is placed in the inner lining 2 of the heat preservation box; the sample holder 3 to be tested is made of a material with high thermal conductivity such as gold, copper, aluminum, or graphene;

[0044] like Figure 4 As shown, the insulation box cover 4 is provided with a sealing strip edge 41, and the sealing strip edge 41 is used to press the sealing strip 6. The insulation box cover 4 is connected to the insulation box shell 1 through the motor drive device 5, and the motor drive device 5 drives the insulation box cover 4 to flip and realize the opening and closing of the insulation box cover 4.

[0045] The opening and closing of the insulation box cover 4 can be achieved through external computer control. The computer is connected to the temperature control unit through a communication line to control the motor drive device 5 to work and complete the opening and closing actions. The temperature control unit is equipped with a zeroing sensor for the insulation box cover 4, and the zeroing position is the closed position of the insulation box cover 4. When the device is turned on and the temperature control unit is powered on, the insulation box cover 4 performs an initialization action, and the initialization is the zeroing action. The zeroing sensor reaches the zero position, the insulation box cover 4 is zeroed, and the insulation box cover 4 is closed. When the computer sends an opening command, the motor drive device 5 performs an opening action. When the computer sends a closing command, the motor drive device 5 performs a closing action.

[0046] In addition, the opening and closing of the heat preservation box cover 4 can also adopt the common automatic sensing switch control method, etc., to facilitate the use of the staff, and the utility model does not make any limitation.

[0047] For example, Figure 3 As shown, the sample holder 3 includes a holder body 31, a boss 32, and a positioning hole 33. A plurality of bosses 32 are arranged in multiple rows on the upper surface of the holder body 3. The positioning hole 33 is provided between two adjacent bosses 32 arranged in the same row. The test tube containing the sample to be tested is placed in the positioning hole 33, and the top edge of the test tube is clamped on the boss 32. The arrangement of the boss 32 is conducive to taking the test tube.

[0048] The front and rear sides of the bracket body 31 are provided with corresponding embedded grooves 34, which are convenient for the staff to take. The sealing strip groove 11 is provided with an outward protrusion 12 at the position corresponding to the embedded groove 34. The protrusion 12 cooperates with the embedded groove 34 to realize a space, which is convenient for the staff to take out or put in the bracket body 31.

[0049] The position of the sealing strip edge 41 corresponds to the position of the sealing strip groove 11, and the sealing strip edge 41 has the same shape as the sealing strip groove 11. The sealing strip edge 41 cooperates with the design of pressing the sealing strip 6, which is conducive to improving the sealing effect.

[0050] For example, Figure 5 As shown, the motor drive device 5 includes a drive motor 51 and a transmission mechanism 52 , the drive motor 51 is transmission-connected to the transmission mechanism 52 , and the transmission mechanism 52 is transmission-connected to the heat-insulating box cover 4 .

[0051] In the above-mentioned temperature control unit, the insulation box lining 2 is placed in the insulation box outer shell 1, the sealing strip 6 is placed in the sealing strip groove 11 of the insulation box outer shell 1, the sample holder 3 to be tested is placed in the insulation box lining 2, the motor compartment outer shell is connected to the insulation box outer shell 1, the insulation box cover 4 is installed on the transmission mechanism 52, and can be buckled on the insulation box outer shell 1, so that the sealing strip pressing edge 41 on the insulation box cover 4 is just pressed on the sealing strip 6.

[0052] Under the movement of the driving motor 51, the heat preservation box cover 4 is turned over through the transmission mechanism 52 to open and close the heat preservation box cover 4. After closing, the sealing strip pressing edge 41 presses on the sealing strip 6 to form a seal, thereby improving the heat preservation effect.

[0053] like Figure 6-Figure 8 As shown, the utility model also provides a temperature control system applied to a sample to be tested by an SPR detector, including the temperature control unit 7 applied to the sample to be tested by the SPR detector, and also includes an SPR detection chamber and a primary temperature control device 8, a secondary temperature control device 9, and a central controller;

[0054] like Figure 7 As shown, the primary temperature control device 8 is arranged in the SPR detection chamber 10, including a heat conduction platform 82, a first temperature regulator 81 and a first temperature sensor 83. The temperature control unit 7 is fixedly placed on the heat conduction platform 82, and the fixing method can be screw connection. The first temperature regulator 81 is arranged below the heat conduction platform 82 to control the temperature of the heat conduction platform 82. The heat conduction platform 82 transmits the temperature to the sample to be tested through the insulation box lining 2 and the sample holder 3 in turn. The first temperature sensor 83 is arranged at the bottom of the temperature control unit 7 to collect the temperature of the temperature control unit 7;

[0055] like Figure 8 As shown, the secondary temperature control device 9 includes a second temperature regulator 91 and a second temperature sensor 92. The second temperature regulator 91 is arranged behind the chamber body of the SPR detection chamber 10, and is used to adjust the ambient temperature in the SPR detection chamber 10. The second temperature sensor 92 is arranged in the SPR detection chamber 10, and is used to collect the ambient temperature in the SPR detection chamber 10. The SPR detection chamber 10 is provided with a chamber door 101.

[0056] The first temperature controller 81 , the first temperature sensor 83 , the second temperature controller 91 , and the second temperature sensor 92 are electrically connected to the central controller, respectively.

[0057] The first temperature controller 81 and the second temperature controller 91 are refrigerators. There are many cooling methods, such as cooling plates, water cooling, air cooling, compressor cooling, etc.

[0058] The primary temperature control device 8 provides the required cooling capacity for the entire temperature control system, and the cooling capacity is transferred to the sample to be tested through the insulation box lining 2 and the sample holder 3; after the insulation box cover 4 is closed, the temperature control unit 7 forms a sealed state, and the temperature inside the temperature control unit 7 can be kept constant for a long time. The secondary temperature control device 9 provides a secondary refrigeration system for the entire system, which can control the ambient temperature outside the temperature control unit 7 and inside the SPR detection chamber 10 at the required temperature. When the insulation box cover 4 is opened, the temperature inside the temperature control unit 7 will change, and as the insulation box cover 4 is opened for an increasing amount of time, its temperature will gradually approach the ambient temperature. This system can effectively control the temperature of the sample to be tested within the required range and can maintain the temperature for a long time.

[0059] The central controller may be an external computer for centralized control of the entire SPR detector and the primary temperature control device 8 and the secondary temperature control device 9 .

[0060] For example, Figure 9-10 As shown, from the analysis of the control principle, the system may include: a monitoring module 9-1, a control module 9-2, an execution module 9-3, and an environment module 9-4.

[0061] The monitoring module 9-1 is connected to the temperature measurement object 1 to measure the temperature, and includes the first temperature sensor 83 and the voltage converter 12. Specifically, the first temperature sensor 83 is connected to the temperature control unit 7, and outputs a voltage corresponding to the current passing through the temperature control unit 7 through the voltage converter 12, and the output voltage has a value corresponding to the temperature inside the temperature control unit 7.

[0062] The control module 9-2, i.e., the central controller, is used to compare the temperature measurement value measured by the input module with the target temperature setting value to issue a heating or cooling instruction; it includes a CPU 21 and a voltage A / D converter 22. The control module 9-2 receives the measurement value through the monitoring module 1, converts the data through the voltage A / D converter 22, compares the converted value with the target value in the CPU 21, and then when the temperature measurement value and the target value are different from each other, the control module 9-2 calculates the adjustment value to match the temperature measurement value with the target value.

[0063] The execution module 9-3 performs heating or cooling operations according to the instructions of the control module 9-2, including the refrigerator 81 and the refrigerator 91. The execution module 3 receives the instructions of the control module 2 and starts the first temperature controller 81 and the second temperature controller 91. According to the control amount, the power of the temperature control is adjusted.

[0064] The environmental module 9-4 is connected to the temperature measurement object 2 to measure the temperature. It includes a second temperature sensor 92 and a voltage converter 12. Specifically, the second temperature sensor 92 is connected to the SPR detection chamber 10, and the voltage converter 12 outputs a voltage corresponding to the current passing through the SPR detection chamber 10, and the output voltage has a value corresponding to the temperature in the SPR detection chamber 10.

[0065] In addition, the utility model also provides a temperature control method for a sample to be tested in an SPR detector, which is applicable to the temperature control system for the sample to be tested in the SPR detector, and comprises the following steps:

[0066] Place the temperature control unit in the SPR detection chamber;

[0067] A first temperature sensor collects the temperature of the temperature control unit and transmits it to the central controller;

[0068] A second temperature sensor collects the ambient temperature in the SPR detection chamber and transmits it to the central controller;

[0069] The central controller controls the first temperature controller and / or the second temperature controller to cool or heat according to the set temperature target value. For example, the temperature in the SPR detection chamber is set to 18°C, and the temperature in the control unit is set to 4°C.

[0070] In summary, the utility model is composed of a multi-stage temperature control system, and each stage of temperature control is independently controlled and affects each other. The temperature of the sample to be tested can be controlled at 4-8°C to ensure that the sample to be tested maintains sufficient activity during the test, so that its test state is closer to the actual use requirements; the temperature of the sample to be tested is effectively controlled within the required range and can be maintained for a long time. This utility model uses a smaller power to achieve a greater effect through this superposition method.

[0071] This article uses specific examples to illustrate the principles and implementation methods of the utility model. The above examples are only used to help understand the method and core ideas of the utility model. At the same time, for those skilled in the art, according to the ideas of the utility model, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the utility model.

Claims

1. A temperature control unit for a sample to be tested in an SPR detector, the temperature control unit being placed in an SPR detection chamber, characterized in that: The temperature control unit comprises: an insulation box shell (1), an insulation box lining (2), a sample holder to be tested (3) and an insulation box cover (4); The heat preservation box shell (1) is provided with a sealing strip groove (11), a sealing strip (6) is arranged in the sealing strip groove (11), and a motor drive device (5) is arranged at the rear end of the heat preservation box shell (1); The heat preservation box lining (2) is nested in the heat preservation box outer shell (1); The sample holder (3) to be tested is placed in the inner lining (2) of the heat preservation box; The heat-insulating box cover (4) is provided with a sealing strip pressing edge (41), the sealing strip pressing edge (41) being used to press the sealing strip (6), the heat-insulating box cover (4) being connected to the heat-insulating box housing (1) via the motor drive device (5), the motor drive device (5) driving the heat-insulating box cover (4) to flip, thereby realizing the opening and closing of the heat-insulating box cover (4).

2. The temperature control unit for the sample to be tested in the SPR detector according to claim 1, characterized in that: The sample holder (3) comprises a holder body (31), a boss (32), and a positioning hole (33); a plurality of the bosses (32) are arranged in a plurality of rows on the upper surface of the holder body (31); the positioning hole (33) is provided between two adjacent bosses (32) arranged in the same row; a test tube containing the sample to be tested is placed in the positioning hole (33), and the top edge of the test tube is clamped on the boss (32).

3. The temperature control unit for the sample to be tested in the SPR detector according to claim 2, characterized in that: The front and rear side surfaces of the bracket body (31) are correspondingly provided with embedded grooves (34).

4. The temperature control unit for the sample to be tested in the SPR detector according to claim 3, characterized in that: An outward protrusion (12) is provided at a position of the sealing strip groove (11) corresponding to the embedded groove (34).

5. The temperature control unit for the sample to be tested in the SPR detector according to claim 1, characterized in that: The position of the sealing strip edge (41) corresponds to the position of the sealing strip groove (11), and the sealing strip edge (41) and the sealing strip groove (11) have the same shape.

6. The temperature control unit for the sample to be tested in the SPR detector according to claim 1, characterized in that: The motor drive device (5) comprises a drive motor (51) and a transmission mechanism (52); the drive motor (51) is in transmission connection with the transmission mechanism (52); and the transmission mechanism (52) is in transmission connection with the heat preservation box cover (4).

7. The temperature control unit for the sample to be tested in the SPR detector according to claim 1, characterized in that: The heat preservation box lining (2) and the sample support (3) to be tested are made of a material with high thermal conductivity, and the material with high thermal conductivity is gold, copper, aluminum or graphene.

8. A temperature control system for a sample to be tested in an SPR detector, characterized in that: A temperature control unit for a sample to be tested in an SPR detector according to any one of claims 1 to 7, further comprising an SPR detection chamber, a primary temperature control device (8), a secondary temperature control device (9), and a central controller; The primary temperature control device (8) is arranged in the SPR detection chamber (10), and comprises a heat conduction platform (82), a first temperature controller (81) and a first temperature sensor (83); the temperature control unit is fixedly placed on the heat conduction platform (82); the first temperature controller (81) is arranged below the heat conduction platform (82) and is used to control the temperature of the heat conduction platform (82); the heat conduction platform (82) transmits the temperature to the sample to be tested through the lining (2) of the heat preservation box and the sample holder (3) in sequence; the first temperature sensor (83) is arranged at the bottom of the temperature control unit (7) and is used to collect the temperature of the temperature control unit (7); The secondary temperature control device (9) comprises a second temperature controller (91) and a second temperature sensor (92), wherein the second temperature controller (91) is arranged behind the chamber body of the SPR detection chamber (10) and is used to control the ambient temperature in the SPR detection chamber (10), and the second temperature sensor (92) is arranged in the SPR detection chamber (10) and is used to collect the ambient temperature in the SPR detection chamber (10); The first temperature controller (81), the first temperature sensor (83), the second temperature controller (91) and the second temperature sensor (92) are respectively electrically connected to the central controller.

9. The temperature control system for a sample to be tested in an SPR detector according to claim 8, characterized in that: The first temperature controller (81) and the second temperature controller (91) are refrigerators.

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