Mobile phase pretreatment device of high performance liquid chromatograph

Abstract

The utility model relates to the technical field of medicine inspection, in particular to a mobile phase pretreatment device of a high performance liquid chromatograph, which comprises a temperature-control light-shielding box, a plurality of positioning mechanisms, a plurality of flow guide plates and a plurality of flow guide plates, the liquid phase bottle is positioned and arranged on the positioning mechanism and soaked in the fluid medium, the liquid phase bottle is provided with a liquid level sensor and a liquid inlet pipe, and the liquid inlet pipe is led out of the liquid phase bottle, soaked in the fluid medium and penetrates out of the side wall of the temperature control light shielding box so as to introduce the mobile phase liquid into the liquid chromatograph; the height adjusting bracket is arranged at the bottom of the temperature control light shielding box so as to support and adjust the working height of the temperature control light shielding box; the constant temperature assembly comprises a heating rod and a temperature sensor which are arranged in the temperature control light shielding box; and the controller is connected to the liquid level sensor, the heating rod and the temperature sensor. According to the scheme, constant-temperature protection, light-proof storage and liquid level monitoring of the mobile phase are realized, so that the accuracy of an analysis result and the use convenience of equipment are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of drug inspection, in particular to a mobile phase pretreatment device for a high performance liquid chromatograph. Background Art

[0002] In pharmaceutical research and quality control, high performance liquid chromatography (HPLC) plays a central role and is essential for the precise analysis of complex compounds. However, the effectiveness of HPLC analysis depends significantly on the stability and consistency of the mobile phase, which is a mixture of solvents in a specific ratio and has a direct impact on separation efficiency, detection sensitivity and column life.

[0003] The main challenge currently faced is that the mobile phase is easily affected by external environmental factors, especially temperature fluctuations and light: multi-component mobile phases may precipitate at low temperatures and clog the system; temperature differences cause sample peak times to deviate from the standard, affecting analysis accuracy; light not only promotes the reproduction of microorganisms in the aqueous phase, but also induces polymerization or oxidation of the organic phase, and even changes the pH value of the buffer salt solution, seriously interfering with experimental results.

[0004] In current practice, mobile phases are usually stored in unprotected transparent containers and directly exposed to the laboratory environment, which undoubtedly increases the risks of temperature and light and limits the reliability and repeatability of the analysis. Utility Model Content

[0005] The utility model provides a mobile phase pretreatment device for a high performance liquid chromatograph, which is used to solve the problems of precipitation of the mobile phase due to temperature changes, unstable peak time, deterioration and contamination caused by light, and realizes constant temperature protection, light-proof storage and liquid level monitoring of the mobile phase, so as to improve the accuracy of the analysis results and the convenience of equipment use.

[0006] The utility model provides a mobile phase pretreatment device for a high performance liquid chromatograph, comprising: a temperature-controlled light-proof box, in which a fluid medium is contained and a plurality of positioning mechanisms are provided; a liquid phase bottle, which is positioned and arranged on the positioning mechanism and immersed in the fluid medium, the liquid phase bottle is provided with a liquid level sensor and a liquid inlet pipe, the liquid inlet pipe is led out from the liquid phase bottle and immersed in the fluid medium, and passes through the side wall of the temperature-controlled light-proof box to introduce the mobile phase liquid into the liquid chromatograph; a height adjustment bracket, which is arranged at the bottom of the temperature-controlled light-proof box to support and adjust the working height of the temperature-controlled light-proof box; a constant temperature component, which comprises a heating rod and a temperature sensor arranged in the temperature-controlled light-proof box; and a controller, which is connected to the liquid level sensor, the heating rod and the temperature sensor.

[0007] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, the liquid phase bottle comprises a light-shielding bottle body and a light-shielding bottle cap sealed on the opening of the light-shielding bottle body; the connecting wires of the liquid inlet pipe and the liquid level sensor respectively enter the light-shielding bottle body through reserved holes on the light-shielding bottle cap.

[0008] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, the temperature-controlled light-proof box comprises a light-shielding box body with an upper opening and a light-shielding box cover capable of closing the light-shielding box body; the outer surfaces of the light-shielding box body and the light-shielding box cover are both provided with a thermal insulation cotton layer.

[0009] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, a water inlet connected to a water supply pipeline and a drain outlet connected to a drain pipeline are arranged on the side wall of the light shielding box; the water inlet and the drain outlet are respectively provided with valves.

[0010] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, a plurality of through holes are arranged on the side wall on the same side of the light-shielding box body; the liquid inlet pipes respectively connected to the plurality of liquid phase bottles pass through the side wall of the light-shielding box body through the plurality of through holes respectively; a rubber plug is arranged between the through hole and the liquid inlet pipe, and the rubber plug is provided with a through hole allowing the liquid inlet pipe to pass through.

[0011] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, the rubber stopper is conical; an annular groove adapted to the through hole is arranged in the circumferential direction of the rubber stopper, and the inside and outside of the rubber stopper are respectively interference fit with the liquid inlet pipe and the through hole.

[0012] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, the positioning mechanism includes: a first elastic support fixedly arranged on the inner wall of the temperature-controlled light-proof box; a second elastic support arranged opposite to the first elastic support; an adjustment mechanism driving the second elastic support to approach or move away from the first elastic support; and opposite sides of the first elastic support and the second elastic support having an arc-shaped surface adapted to the shape of the liquid phase bottle.

[0013] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, the adjustment mechanism includes: a fixing nut fixedly arranged on the side wall of the temperature-controlled light-proof box opposite to the first elastic support; a threaded column threadedly matched with the fixing nut, the second elastic support being installed on the head of the threaded column, the head of the threaded column having an annular groove embedded in a locking mechanism on the end face of the second elastic support to allow the threaded column to rotate around its axis relative to the second elastic support; a tightening handle arranged at the tail of the threaded column.

[0014] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, the height adjustment bracket includes: an upper bracket, which is arranged at the bottom of the temperature-controlled light-proof box; a lower bracket, which is arranged parallel to the upper bracket, and a base plate is arranged at the bottom of the lower bracket; two groups of cross arms, which are arranged between the upper bracket and the lower bracket, and a plurality of arm pins are arranged between the two groups of cross arms; a lifting control mechanism is arranged between the lower bracket and the cross arms to adjust the support height of the cross arms.

[0015] According to a mobile phase pretreatment device of a high performance liquid chromatograph provided by the utility model, the lifting control mechanism includes: a fixed ring block, fixedly arranged on the base plate; a lifting screw, rotatably installed on the fixed ring block around an axis, and threadedly engaged with the support arm pin rod at one end of the cross support arm, so as to be able to convert the rotational movement of the lifting screw into a horizontal movement movement of the end of the cross support arm; on the outer side of the fixed ring block, a lifting handle for rotating the lifting screw is arranged at the end of the lifting screw.

[0016] The mobile phase pretreatment device for a high-performance liquid chromatograph provided by the utility model is used to maintain a constant temperature of the mobile phase in the liquid phase bottle and the liquid inlet tube through a temperature-controlled light-proof box with a built-in fluid medium. The box is equipped with a multi-point positioning mechanism to stably store the liquid phase bottle. The liquid phase bottle is equipped with a built-in liquid level sensor to monitor the liquid inventory in real time to prevent the pump from sucking it empty. A height adjustment bracket is configured at the bottom of the device to flexibly adapt to the height of different types of chromatographs, ensure smooth docking, shorten the length of externally exposed pipelines, and reduce the influence of external temperature differences. The constant temperature component contains a heating rod and a temperature sensor to accurately control the temperature in the box. Through unified management by the controller, the liquid level and temperature are monitored synchronously, and the heating is automatically adjusted, thereby providing a stable and controllable mobile phase processing environment, effectively solving the problems caused by temperature fluctuations and light in traditional methods, and improving the stability and reliability of drug detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 It is a structural schematic diagram of a mobile phase pretreatment device of a high performance liquid chromatograph provided by the utility model, in which a temperature-controlled light-proof box is partially perspectively displayed to show a liquid phase bottle inside the box.

[0019] Figure 2It is a schematic diagram of the structure of a temperature-controlled light-proof box and a height-adjustable bracket of a mobile phase pretreatment device.

[0020] Figure 3 It is a schematic diagram of the structure of the temperature-controlled light-proof box and controller of the mobile phase pretreatment device from a top-down perspective.

[0021] Figure 4 It is a schematic diagram of the side cross-sectional structure of a temperature-controlled light-proof box and a height-adjustable bracket of a mobile phase pretreatment device.

[0022] Figure 5 It is a schematic diagram of the structure of the height adjustment bracket of the mobile phase pretreatment device.

[0023] Figure 6 It is a partial structural schematic diagram of the positioning mechanism of the mobile phase pretreatment device.

[0024] Reference numerals:

[0025] 10. Temperature-controlled light-proof box; 11. Water inlet; 12. Drain outlet; 13. Through hole; 14. Rubber plug; 20. Liquid phase bottle; 21. Liquid level sensor; 22. Liquid inlet pipe; 30. Height-adjusting bracket; 31. Upper bracket; 32. Lower bracket; 33. Base plate; 34. Cross support arm; 35. Support arm pin rod; 36. Fixed ring block; 37. Lifting screw; 38. Lifting handle; 41. Heating rod; 42. Temperature sensor; 50. Controller; 61. First elastic support; 62. Second elastic support; 63. Fixing nut; 64. Threaded column; 65. Tightening handle; 66. Locking mechanism. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] In the description of the embodiments of the present utility model, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate the orientation or position relationship based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the embodiments of the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present utility model. In addition, the terms "first", "second", and "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. It should also be noted that in the description of the present utility model, unless otherwise clearly specified and limited, the terms "set", "install", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection. For those skilled in the art, the specific meanings of the above terms in the present utility model can be understood according to the specific circumstances.

[0028] The utility model provides a mobile phase pretreatment device for a high performance liquid chromatograph, which cleverly integrates temperature control and light-proof properties. The mobile phase bottle is embedded in an intelligent temperature-controlled light-proof box, and with the help of a circulating constant temperature water system, it ensures that the mobile phase maintains the target temperature from beginning to end, effectively preventing temperature difference precipitation and eliminating peak deviation. The fully enclosed light-proof design eliminates photolysis reactions and improves analytical stability. The device has a built-in liquid level monitoring system to track the mobile phase capacity in real time, prevent the pump from idling, and prompt the user to intervene in time. In addition, the unique height adjustment mechanism is suitable for various types of chromatograph models, optimizes the mobile phase delivery path, shortens the length of the exposed pipeline, further isolates the interference of environmental temperature changes, and ensures constant temperature transmission of the mobile phase, providing an accurate and efficient solution for drug analysis.

[0029] Combine the following Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 The specific implementation of the mobile phase pretreatment device of the high performance liquid chromatograph of the utility model is described.

[0030] The utility model provides a mobile phase pretreatment device for a high performance liquid chromatograph, comprising: a temperature-controlled light-proof box 10, in which a fluid medium is contained and a plurality of positioning mechanisms are provided; a liquid phase bottle 20, which is positioned and arranged on the positioning mechanism and immersed in the fluid medium, the liquid phase bottle 20 is provided with a liquid level sensor 21 and a liquid inlet pipe 22, the liquid inlet pipe 22 is led out from the liquid phase bottle 20 and immersed in the fluid medium, and passes through the side wall of the temperature-controlled light-proof box 10 to introduce the mobile phase liquid into the liquid chromatograph; a height adjustment bracket 30, which is arranged at the bottom of the temperature-controlled light-proof box 10 to support and adjust the working height of the temperature-controlled light-proof box 10; a constant temperature component, comprising a heating rod 41 and a temperature sensor 42 arranged in the temperature-controlled light-proof box 10; and a controller 50, which is connected to the liquid level sensor 21, the heating rod 41 and the temperature sensor 42.

[0031] Specifically, the temperature-controlled light-proof box 10 is filled with a fluid medium, which not only provides a constant temperature storage environment for the liquid phase bottle 20 and the liquid inlet pipe 22, but also effectively isolates external light and avoids the decomposition of light-sensitive substances. The multiple positioning mechanisms carefully arranged in the box ensure the precise placement of the liquid phase bottle 20, and at least a portion of the liquid phase bottle 20 and the liquid inlet pipe 22 are immersed in the fluid medium. Each liquid phase bottle 20 is built with a liquid level sensor 21 to monitor the liquid level of the mobile phase in the bottle in real time. Once it approaches the minimum safety line, a refill reminder is immediately issued through the controller 50 to prevent the experiment from being interrupted. In order to meet the matching requirements of high-performance liquid chromatographs of different models, a height adjustment bracket 30 is installed at the bottom of the device, which can flexibly adjust the working height of the temperature-controlled light-proof box 10 to ensure that it is flush with the chromatograph pump interface, which not only simplifies the connection steps, but also shortens the length of the external exposed pipeline of the temperature-controlled light-proof box 10 as much as possible, and reduces the influence of external temperature fluctuations and light on the mobile phase. The heating rod 41 of the thermostatic assembly is precisely adjusted by the controller 50 according to the feedback information of the temperature sensor 42 to maintain the uniform and stable temperature in the box. Preferably, the heating rod 41 and the temperature sensor 42 are separately arranged to prevent affecting the detection result of the temperature sensor 42. For example, the arrangement positions of the heating rod 41 and the temperature sensor 42 can be respectively close to the side walls of two temperature-controlled light-proof boxes 10 that are far apart.

[0032] The controller 50 preferably includes a control panel, a PLC, a liquid level alarm light, an alarm horn, etc. The PLC is located inside the controller 50, and the control panel is connected to the PLC, which is used to set the target temperature of the fluid medium in the box and the target alarm liquid level of the liquid in the liquid phase bottle 20, and send the set value to the PLC. The PLC is connected to the liquid level sensor 21 and the temperature sensor 42 respectively to monitor the temperature of the fluid medium and the liquid level height of the liquid in the liquid phase bottle 20. The PLC is connected to the heating rod 41 to control heating and shutting down. When the temperature of the fluid medium is lower than the target temperature, the PLC turns on the heating rod 41 for heating, and when the water bath temperature reaches the target temperature, the PLC turns off the heating rod 41 to stop heating. The PLC is connected to the liquid level alarm light and the alarm horn to monitor the liquid level height of the liquid phase bottle 20 and alarm for low liquid level. When the liquid level in a certain numbered liquid phase bottle 20 is lower than the target alarm liquid level height, the PLC controls the corresponding numbered liquid level alarm light to light up, and controls the alarm horn to sound an alarm. Until the liquid is added to the liquid phase bottle 20 and maintained above the target liquid level, the PLC controls the liquid level alarm light of the corresponding number to go out and stops the alarm horn.

[0033] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, the liquid phase bottle 20 includes a light-shielding bottle body and a light-shielding bottle cap sealed at the opening of the light-shielding bottle body; the liquid inlet pipe 22 and the connecting wire of the liquid level sensor 21 enter the light-shielding bottle body through the reserved holes on the light-shielding bottle cap respectively. By using opaque materials to manufacture the bottle body and the bottle cap, external light is effectively blocked, thereby protecting the mobile phase stored in the bottle, avoiding chemical property changes or decomposition caused by light, and ensuring the accuracy and repeatability of the analysis results. In actual operation, in order not to affect the integrity of the light shielding while achieving functional access, a reserved hole is specially designed on the cover of the liquid phase bottle 20. The two reserved holes are used for the insertion of the liquid inlet pipe 22 and the connection wire of the liquid level sensor 21 respectively. The liquid inlet tube 22 is led out from the liquid phase bottle 20, immersed in the fluid medium, and passes through the side wall of the temperature-controlled light-proof box 10 to introduce the mobile phase liquid into the liquid chromatograph; and the liquid level sensor 21 monitors the liquid level in the bottle in real time through the connecting line to ensure sufficient supply of mobile phase during the experiment.

[0034] The light-shielding bottle body is preferably a light-proof glass bottle, and together with the light-shielding bottle cap, it forms a liquid-shielding bottle 20, which is used to store the mobile phase liquid required by the liquid chromatograph to achieve the effect of light-shielding storage and preventing volatilization. In addition, the liquid inlet pipe 22 can also be light-shielded, and is preferably a Teflon hose, which not only has good light-shielding performance, can continue the light-shielding protection of the mobile phase from the bottle body to the chromatograph, and avoid any possible influence of external light on the mobile phase, but also has strong chemical stability, corrosion resistance, does not adsorb samples, and will not cause pollution to the mobile phase.

[0035] According to a mobile phase pretreatment device for a high-performance liquid chromatograph provided by the utility model, a temperature-controlled light-proof box 10 includes a light-shielding box body with an opening at the top and a light-shielding box cover capable of closing the light-shielding box body; the outer surfaces of the light-shielding box body and the light-shielding box cover are both provided with a thermal insulation cotton layer. The open top design of the light-shielding box body makes it convenient for users to put in and take out the liquid phase bottle 20, while the light-shielding box cover can tightly close the box body to form a sealed space, effectively blocking the entry of external light, ensuring that the mobile phase in the box is protected from the influence of light, and avoiding possible chemical reactions or decomposition. In order to further enhance the performance of the device, the outer surfaces of the light-shielding box body and the light-shielding box cover are both covered with a layer of thermal insulation cotton. This layer of thermal insulation cotton can not only effectively isolate the influence of external temperature fluctuations on the environment inside the box, but also maintain the stability of the temperature inside the box.

[0036] The light shielding box is preferably a rectangular box with an upper opening, and the light shielding box cover is buckled on the upper part of the light shielding box to form a complete cavity box. The light shielding box and the light shielding box cover are preferably made of stainless steel, and other strong, non-deformable, rust-proof and leak-proof materials can also be used.

[0037] According to a mobile phase pretreatment device for a high-performance liquid chromatograph provided by the utility model, a water inlet 11 connected to a water supply pipeline and a drain outlet 12 connected to a drain pipeline are arranged on the side wall of the light-shielding box; the water inlet 11 and the drain outlet 12 are respectively provided with valves. A water inlet 11 connected to a water supply pipeline and a drain outlet 12 connected to a drain pipeline are installed on the side wall of the light-shielding box, so that the user can easily inject or discharge a water bath medium for maintaining a constant temperature, such as deionized water or other suitable temperature-controlled liquid, into the light-shielding box. The water inlet 11 and the drain outlet 12 are each equipped with a valve, and the setting of the valve allows the user to accurately control the opening and closing of the water flow, whether it is to inject fresh constant temperature medium into the box, or to discharge old liquid for replacement or cleaning when necessary, it can be easily operated, which is convenient for daily maintenance and long-term maintenance of the system cleanliness, thereby improving the reliability of the experiment and the accuracy of the analysis results.

[0038] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, a plurality of through holes 13 are arranged on the same side wall of the light shielding box; liquid inlet pipes 22 respectively connected to a plurality of liquid phase bottles 20 pass through the side wall of the light shielding box through the plurality of through holes 13; a rubber plug 14 is arranged between the through holes 13 and the liquid inlet pipe 22, and the rubber plug 14 is provided with a through hole allowing the liquid inlet pipe 22 to pass through. The structure of the through holes 13 and the rubber plug 14 not only realizes the orderly arrangement and sealing of the liquid inlet pipe 22, but also further consolidates the light shielding and temperature control capabilities of the entire pretreatment device.

[0039] Among them, the mobile phase in each liquid phase bottle 20 is connected to the liquid chromatograph outside the temperature-controlled light-proof box 10 through its own liquid inlet pipe 22. These liquid inlet pipes 22 enter the box through the corresponding through holes 13 on the side wall of the light-shielding box body and are connected to the liquid phase bottle 20, ensuring that the mobile phase can be safely, effectively, stably and thermostatically extracted. In order to ensure the sealing of the box body and prevent light leakage, a rubber plug 14 is also provided between each through hole 13 and the liquid inlet pipe 22. The rubber plug 14 not only plays a sealing role to prevent mutual interference between the environment inside and outside the box, but also the rubber plug 14 itself is designed with a through hole, so that the liquid inlet pipe 22 can pass through without destroying the sealing effect. This design not only ensures the light-shielding properties of the light-shielding box body, but also ensures the stable connection of the liquid inlet pipe 22, avoiding the influence of external temperature and light on the mobile phase.

[0040] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, the rubber stopper 14 is conical; an annular groove adapted to the through hole 13 is provided in the circumferential direction of the rubber stopper 14, and the inside and outside of the rubber stopper 14 are respectively interference fit with the liquid inlet pipe 22 and the through hole 13. The conical shape is conducive to preventing the rubber stopper 14 from leaking, and is also easy to install and disassemble. An annular groove is provided in the circumferential direction of the rubber stopper 14, and the annular groove is adapted to the size and shape of the through hole 13, ensuring that the liquid inlet pipe 22 can remain stable and not loose when passing through the rubber stopper 14, avoiding displacement caused by vibration or pressure changes during use, thereby improving the stability and durability of the entire system. The inner and outer parts of the rubber stopper 14 are respectively connected to the liquid inlet pipe 22 and the through hole 13 by means of interference fit, that is, there is a slight interference fit between the liquid inlet pipe 22 and the through hole 13 during assembly, so that close compressive contact can be achieved by relying on the elastic deformation force of the material, which not only enhances the sealing effect and prevents the entry of external pollutants or the leakage of internal liquid, but also increases the friction force to ensure that the liquid inlet pipe 22 is not easily displaced due to temperature changes or pressure fluctuations in the working state.

[0041] According to a mobile phase pretreatment device for a high performance liquid chromatograph provided by the utility model, the positioning mechanism includes: a first elastic support 61, fixedly arranged on the inner wall of a temperature-controlled light-proof box 10; a second elastic support 62, arranged opposite to the first elastic support 61; an adjustment mechanism, driving the second elastic support 62 to approach or move away from the first elastic support 61; and the opposite sides of the first elastic support 61 and the second elastic support 62 have an arc-shaped surface adapted to the shape of the liquid phase bottle 20. The positioning mechanism can not only ensure that the liquid phase bottle 20 is firmly placed in the temperature-controlled light-proof box 10 to avoid liquid level fluctuations caused by vibration or movement, but also can realize quick and easy adaptation to liquid phase bottles 20 of different sizes through the adjustment mechanism, thereby improving the versatility and convenience of operation of the device.

[0042] Specifically, the first elastic support 61 is fixedly mounted on the side wall inside the temperature-controlled light-proof box 10 as the basis for supporting the liquid phase bottle 20. It is designed to have an elastic structure that can adapt to and slightly wrap the shape of the liquid phase bottle 20 to provide stable support while avoiding scratching the bottle body. The second elastic support 62 is arranged opposite to the first elastic support 61 to form a clamping space, and the two work together to fix the liquid phase bottle 20. The second elastic support 62 is also designed with an arc-shaped surface that adapts to the shape of the liquid phase bottle 20, which is conducive to the balanced support of the bottle body. The adjustment mechanism can drive the second elastic support 62 to move closer to or away from the first elastic support 61. The existence of this mechanism allows the user to adjust the degree of clamping according to actual needs to adapt to liquid phase bottles 20 of different specifications, or to fine-tune the degree of fixation and tightness of the liquid phase bottle 20 according to experimental conditions to ensure the stability of the experiment.

[0043] According to a mobile phase pretreatment device of a high performance liquid chromatograph provided by the utility model, the adjustment mechanism includes: a fixing nut 63, fixedly arranged on the side wall of the temperature-controlled light-proof box 10 opposite to the first elastic support 61; a threaded column 64, threadedly matched with the fixing nut 63, the second elastic support 62 is installed on the head of the threaded column 64, the head of the threaded column 64 has an annular groove, embedded in the locking mechanism 66 of the end face of the second elastic support 62, so as to allow the threaded column 64 to rotate relative to the second elastic support 62 around its axis; a tightening handle 65, arranged at the tail of the threaded column 64. The adjustment mechanism realizes the rapid positioning and firm fixation of the liquid phase bottle 20 in the pretreatment device through a simple and effective thread adjustment method, while ensuring the versatility of the device and the convenience of operation.

[0044] Specifically, the fixing nut 63 is installed on the inner wall of the temperature-controlled light-shielding box 10 opposite to the first elastic support 61, and is fixed as a part of the adjustment mechanism to provide a support point for the threaded column 64. The threaded column 64 and the fixing nut 63 are mechanically matched through threads, and when the threaded column 64 rotates along its threads, it can move between the fixing nut 63. Figure 6 As shown, the annular groove of the head of the threaded column 64 is embedded in the locking mechanism 66 of the end face of the second elastic support 62, so as to realize the installation of the head of the threaded column 64 and the second elastic support 62. This installation method allows the threaded column 64 to rotate relative to the second elastic support 62 around its axis, so that the second elastic support 62 can adjust the distance relative to the first elastic support 61 with the movement of the threaded column 64 without affecting the angle of cooperation with the bottle body, thereby adapting to the needs of different positions. The second elastic support 62 is installed through the head of the threaded column 64 and can move axially with the threaded column 64. Its movement is controlled by the operation of the tightening handle 65. The tightening handle 65 is set at the tail of the threaded column 64. The user can rotate the threaded column 64 by hand to rotate the handle, thereby making the second elastic support 62 close to or away from the first elastic support 61, so as to achieve the purpose of adjusting the clamping or loosening of the liquid phase bottle 20.

[0045] According to a mobile phase pretreatment device for a high-performance liquid chromatograph provided by the utility model, a height-adjustable bracket 30 includes: an upper bracket 31, arranged at the bottom of a temperature-controlled light-proof box 10; a lower bracket 32, arranged in parallel with the upper bracket 31, and a base plate 33 is arranged at the bottom of the lower bracket 32; two groups of cross arms 34, arranged between the upper bracket 31 and the lower bracket 32, and a plurality of arm pins 35 are arranged between the two groups of cross arms 34; a lifting control mechanism is arranged between the lower bracket 32 ​​and the cross arms 34 to adjust the support height of the cross arms 34. The design of the height-adjustable bracket 30 provides high flexibility and adaptability for achieving seamless docking between the device and liquid chromatographs of different models.

[0046] Specifically, the upper bracket 31 of the height adjustment bracket 30 is directly fixed to the bottom of the temperature-controlled light-proof box 10. The lower bracket 32 ​​is arranged in parallel with the upper bracket 31, maintaining a certain horizontal distance, and a base plate 33 is arranged at the bottom thereof, which ensures the stability and load-bearing capacity of the entire bracket system. Two groups of cross arms 34 are installed between the upper bracket 31 and the lower bracket 32 ​​to form a stable X-shaped structure, which enhances the stability and support strength of the entire device. The arm pin 35 is arranged between the two groups of cross arms 34, and is connected by these pins, which further enhances the connection rigidity and stability between the arms, ensuring that no unnecessary shaking or deformation occurs when adjusting the height. The lifting control mechanism is arranged between the lower bracket 32 ​​and the cross arms 34, which is the core of adjusting the height. Through the operation of this mechanism, the user can accurately adjust the support height of the cross arms 34, thereby changing the overall height of the temperature-controlled light-proof box 10, so that it is perfectly matched with the height of the liquid inlet of the liquid chromatograph. The lifting control mechanism can be in various forms such as manual knob, air pressure or electric, and is specifically designed according to actual application requirements.

[0047] Further, according to a mobile phase pretreatment device of a high performance liquid chromatograph provided by the utility model, the lifting control mechanism includes: a fixed ring block 36, fixedly arranged on a base plate 33; a lifting screw 37, rotatably mounted on the fixed ring block 36 around an axis, and threadedly matched with a support arm pin 35 at one end of a cross arm 34, so as to be able to convert the rotational action of the lifting screw 37 into a horizontal movement action of the end of the cross arm 34; on the outer side of the fixed ring block 36, a lifting handle 38 for rotating the lifting screw 37 is provided at the end of the lifting screw 37. Specifically, the fixed ring block 36 is firmly mounted on the base plate 33, serving as a support point for the rotation of the lifting screw 37, thereby ensuring the stability of the entire lifting mechanism. The lifting screw 37 is rotatably mounted on the fixed ring block 36, and can freely rotate around its own axis. One end of the lifting screw 37 and the support arm pin 35 at one end of the cross arm 34 are threadedly matched with each other. This means that when the lifting screw 37 rotates, its linear motion is converted into a push-pull force in the horizontal direction through the thread, thereby driving the cross arm 34 to rise or fall, thereby achieving precise height adjustment. The lifting handle 38 is located outside the fixed ring block 36 and is directly installed at the end of the lifting screw 37. The user only needs to turn the handle to easily control the up and down movement of the lifting screw 37, thereby adjusting the height of the pretreatment device, which is convenient for the user's operation. No tools are required, and the height can be precisely adjusted with one hand.

[0048] Preferably, Figure 5 As shown, the two upper brackets 31 of the height adjustment bracket 30 are fixed on both sides of the bottom plate of the temperature-controlled light-proof box 10, with a pin hole on the left side and a long hole on the right side. The two lower brackets 32 are fixed on a solid base plate 33, with a pin hole on the left side and a long hole on the right side. Two groups of cross arms 34 are respectively installed between the upper bracket 31 and the lower bracket 32, and the left end of the cross arm 34 is fixed to the pin hole of the upper and lower brackets 32 through two arm pins 35, and the pin can rotate in the pin hole; then the right end of the cross arm 34 is installed in the long hole of the upper and lower brackets 32 through two arm pins 35, and the pin can slide left and right in the long hole. The fixed ring block 36 is welded to the base plate 33, and the lifting screw 37 passes through the fixed ring block 36 and is stuck in its hole, which can rotate but not fall off. The lifting screw 37 is screwed into the threaded hole in the middle of the support arm pin 35 in the long hole on the lower right side, and the lifting handle 38 at the end of the lifting screw 37 is rotated. The lifting screw 37 is screwed in and out to push the support arm pin 35 in the long hole to move left and right, thereby realizing the raising and lowering of the height adjustment bracket 30, and then realizing the height adjustment of the pretreatment device.

[0049] According to the mobile phase pretreatment device of the high performance liquid chromatograph of the preferred embodiment of the utility model, it is placed adjacent to the liquid chromatograph, and the height of the pretreatment device is adjusted by rotating the lifting handle 38 through the height adjustment bracket 30 to be flush with the height of the liquid inlet of the liquid chromatograph pump or higher than the position of the instrument pump, so that the mobile phase liquid can flow more smoothly, and the length of the liquid inlet pipe 22 between the pretreatment device and the liquid chromatograph is the shortest, which minimizes the temperature influence of the external environment on the mobile phase liquid and ensures constant temperature stability. The liquid phase bottle 20 is placed in the temperature-controlled light-proof box 10, and the tightening handle 65 of the adjustment mechanism is rotated, and the threaded column 64 is screwed in to push the second clamping support, and the first clamping support is used to clamp and fix the liquid phase bottle 20. Then water is added to more than half the height of the liquid phase bottle 20 through the water inlet 11, but there is still a certain distance from the bottle mouth, so that the water does not contact the bottle mouth to affect the composition of the mobile phase liquid.

[0050] The controller 50 is powered on, and the controller 50 supplies power to the liquid level sensor 21, the heating rod 41 and the temperature sensor 42 through the cable. The experimenter sets the mobile phase liquid temperature required for the experiment as the target temperature through the control panel of the controller 50, sets a lower liquid level height that can continue to be used for a period of time as the target alarm liquid level, and the control panel sends the set value to the PLC. The PLC stores a pre-programmed control logic. When the water bath temperature is lower than the target temperature, the PLC turns on the heating rod 41 for heating, and when the water bath temperature reaches the target temperature, the PLC turns off the heating rod 41 to stop heating, so that the temperature of the water bath can be constant at the target temperature, generally 35-45°C. Since the liquid phase bottle 20 and the liquid inlet pipe 22 are immersed in the water bath, the mobile phase liquid therein will also reach and be constant at the target temperature after a certain length of time. The heat insulation cotton on the outer surface of the light-shielding box body and the light-shielding box cover can play a good role in heat insulation, can reduce the heat loss of the water bath, save energy and stabilize the temperature. It solves the problem of component separation caused by low temperature of the mobile phase liquid or temperature change causing deviation of the test results. When the liquid level in a certain numbered liquid phase bottle 20 is lower than the target alarm level, the PLC controls the corresponding numbered liquid level alarm light to light up and the alarm speaker to sound an alarm. When the mobile phase in this liquid phase bottle 20 is added to above the target liquid level, the PLC controls the corresponding numbered liquid level alarm light to go out and stops the alarm speaker from sounding. In the case where the experimenter cannot directly observe the liquid level of the liquid phase bottle 20, automatic monitoring and alarm of the liquid level can be realized, and the experimenter can be relieved from having to monitor the equipment throughout the process.

[0051] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "mode", "specific mode", or "some modes" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or mode are included in at least one embodiment or mode of the utility model embodiment. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or mode. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or modes in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or modes described in this specification and the features of the different embodiments or modes, without contradiction.

[0052] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A mobile phase pretreatment device for a high performance liquid chromatograph, characterized in that: include: A temperature-controlled light-proof box (10) containing a fluid medium and provided with a plurality of positioning mechanisms; A liquid phase bottle (20) is positioned on the positioning mechanism and immersed in the fluid medium. The liquid phase bottle (20) is provided with a liquid level sensor (21) and a liquid inlet pipe (22). The liquid inlet pipe (22) is led out from the liquid phase bottle (20) and immersed in the fluid medium, and passes through the side wall of the temperature-controlled light-proof box (10) to introduce the mobile phase liquid into the liquid chromatograph; a height adjustment bracket (30) is arranged at the bottom of the temperature-controlled light-proof box (10) to support and adjust the working height of the temperature-controlled light-proof box (10); A constant temperature component, comprising a heating rod (41) and a temperature sensor (42) arranged in the temperature-controlled light-proof box (10); A controller (50) is connected to the liquid level sensor (21), the heating rod (41) and the temperature sensor (42).

2. The mobile phase pretreatment device for high performance liquid chromatograph according to claim 1, characterized in that: The liquid phase bottle (20) comprises a light-shielding bottle body and a light-shielding bottle cap sealed on the opening of the light-shielding bottle body; The connecting wires of the liquid inlet pipe (22) and the liquid level sensor (21) respectively enter the light-shielding bottle body through the reserved holes on the light-shielding bottle cover.

3. The mobile phase pretreatment device for high performance liquid chromatograph according to claim 1, characterized in that: The temperature-controlled light-proof box (10) comprises a light-proof box body with an upper opening and a light-proof box cover capable of closing the light-proof box body; The outer surfaces of the light-shielding box body and the light-shielding box cover are both provided with a heat-insulating cotton layer.

4. The mobile phase pretreatment device for high performance liquid chromatograph according to claim 3, characterized in that: A water inlet (11) connected to a water supply pipeline and a drainage outlet (12) connected to a drainage pipeline are provided on the side wall of the light shielding box; The water inlet (11) and the water outlet (12) are respectively provided with valves.

5. The mobile phase pretreatment device for high performance liquid chromatograph according to claim 3, characterized in that: A plurality of through holes (13) are arranged on the same side wall of the light shielding box; The liquid inlet pipes (22) respectively connected to the plurality of liquid phase bottles (20) respectively pass through the side wall of the light shielding box through the plurality of through holes (13); A rubber stopper (14) is provided between the through hole (13) and the liquid inlet pipe (22), and the rubber stopper (14) is provided with a through hole that allows the liquid inlet pipe (22) to pass through.

6. The mobile phase pretreatment device for high performance liquid chromatograph according to claim 5, characterized in that: The rubber stopper (14) is conical; The rubber stopper (14) is provided with an annular groove adapted to the through hole (13) in the circumferential direction, and the inside and outside of the rubber stopper (14) are respectively interference-fitted with the liquid inlet pipe (22) and the through hole (13).

7. The mobile phase pretreatment device for high performance liquid chromatography according to any one of claims 1 to 6, characterized in that: The positioning mechanism comprises: A first elastic support (61) is fixedly arranged on the inner wall of the temperature-controlled light-proof box (10); A second elastic support (62), arranged opposite to the first elastic support (61); An adjustment mechanism drives the second elastic support (62) to move closer to or farther away from the first elastic support (61); The opposite sides of the first elastic support (61) and the second elastic support (62) have arc-shaped surfaces adapted to the shape of the liquid phase bottle (20).

8. The mobile phase pretreatment device for high performance liquid chromatograph according to claim 7, characterized in that: The regulating mechanism comprises: A fixing nut (63) fixedly arranged on a side wall of the temperature-controlled light-shielding box (10) opposite to the first elastic support (61); A threaded column (64) is threadably matched with the fixing nut (63); the second elastic support is mounted on the head of the threaded column (64); the head of the threaded column (64) has an annular groove which is embedded in a locking mechanism (66) on the end surface of the second elastic support (62) to allow the threaded column (64) to rotate around its axis relative to the second elastic support (62); A tightening handle (65) is arranged at the tail of the threaded column (64).

9. The mobile phase pretreatment device for high performance liquid chromatography according to any one of claims 1 to 6, characterized in that: The height adjustment bracket (30) comprises: An upper bracket (31) is arranged at the bottom of the temperature-controlled light-proof box (10); A lower bracket (32) is arranged parallel to the upper bracket (31), and a base plate (33) is arranged at the bottom of the lower bracket (32); Two groups of cross arms (34) are arranged between the upper support (31) and the lower support (32), and a plurality of arm pins (35) are arranged between the two groups of cross arms (34); A lifting control mechanism is provided between the lower support (32) and the cross support arm (34) to adjust the support height of the cross support arm (34).

10. The mobile phase pretreatment device for high performance liquid chromatograph according to claim 9, characterized in that: The lifting control mechanism comprises: A fixed ring block (36) fixedly arranged on the base plate (33); A lifting screw (37) is rotatably mounted on the fixed ring block (36) around an axis and is threadedly engaged with the arm pin (35) at one end of the cross arm (34) so ​​as to convert the rotational movement of the lifting screw (37) into a horizontal movement of the end of the cross arm (34); On the outer side of the fixed ring block (36), a lifting handle (38) for rotating the lifting screw (37) is arranged at the end of the lifting screw (37).

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