Cell culture appliance with drainage function
By designing cell culture instruments with drainage function, the problem of difficult to control the amount of culture medium in traditional cell culture is solved, and a more efficient cell culture and a sterile environment is achieved.
Patent Information
- Application Number
- CN202421647053.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-11
- Publication Date
- 2025-05-02
- Estimated Expiration
- 2034-07-11
AI Technical Summary
During traditional cell culture, it is difficult to control the amount of culture medium, which affects the growth and health of cells.
A cell culture device with drainage function is designed, including a controllable culture box and multiple culture vessels. The drainage speed and amount of the culture medium are controlled through the drainage plate, the culture plate and the control assembly.
By precisely controlling the amount of culture medium, it provides more biologically similar culture conditions, improves the efficiency of cells, and ensures sterile environment and safety issues.
Smart Images
Figure CN222821577U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture apparatus, in particular to a cell culture apparatus with a drainage function. Background Art
[0002] Cell culture refers to the process of cell growth and proliferation by placing cells in a culture medium containing appropriate nutrients under constant temperature, constant humidity and sterile conditions. Cell culture is an important experimental method in biological and medical research, and can be used to study cell biological characteristics, drug screening, gene expression and treatment methods.
[0003] The significance of studying cell culture is that it can provide a controlled and repeatable experimental environment, so as to better understand the physiological functions and reaction mechanisms of cells, and study the occurrence and development of diseases. Through cell culture, scientists can simulate the biological processes in the human body, thereby improving the understanding of disease treatment and prevention, and promoting the development of medicine and biology.
[0004] In the traditional cell culture process, when draining the cell culture fluid, the amount of culture fluid used is often not well controlled. The amount of cell culture fluid will have a significant impact on the growth and health of the cultured cells. Too little cell culture fluid will cause the cells to be unable to obtain sufficient nutrients and water, thereby affecting their growth and proliferation; while too much cell culture fluid may cause excessive dilution of cells, affecting the interaction between cells and nutrient uptake. Therefore, the utility model proposes a cell culture device with drainage function to ensure the appropriate amount of cell culture fluid. Utility Model Content
[0005] The utility model aims to provide a cell culture device with a drainage function. When draining cell culture fluid, the drainage function is used to control the liquid inlet speed and liquid inlet amount, control the usage of the culture fluid, and improve the cell culture efficiency.
[0006] To achieve the above-mentioned purpose, the utility model adopts the following technical solution: a cell culture apparatus with drainage function, comprising a controllable culture box and a plurality of culture vessels arranged inside the controllable culture box;
[0007] The controllable culture box comprises a drainage plate, a culture plate connected to the lower surface of the drainage plate, and a control component connected to the drainage plate and the side of the culture plate;
[0008] The culture vessel comprises a culture bottle and a liquid outlet component arranged at the outer bottom of the culture bottle.
[0009] Optionally, the drainage plate is configured as a square structure, a plurality of drainage grooves of cylindrical structure are evenly arranged in the middle of the drainage plate, a flexible drainage tube is arranged on the inner side of the drainage groove, a drainage channel connected to the drainage groove is arranged inside the drainage plate, a plurality of switch knobs connected to the drainage channel are arranged on the upper surface of the drainage plate, and two clamping strips are arranged on the lower surface of the drainage plate.
[0010] Optionally, the drainage channel includes a first drainage channel and a plurality of second drainage channels arranged perpendicular to the first drainage channel, one end of the first drainage channel is connected to the control component, the middle and the other end of the first drainage channel are connected to the second drainage channel, both ends of the second drainage channel are connected to the flexible drainage tube, and the switch knob is arranged at both ends of the second drainage channel.
[0011] Optionally, the culture plate is configured as a square structure with a hollow interior, a plurality of placement areas for placing the culture vessels are evenly arranged on the inner bottom of the culture plate, the placement areas are arranged directly below the drainage groove, and a temperature controller and a temperature sensor are arranged in the placement areas;
[0012] The top of the culture plate is provided with a snap-in groove matched with the snap-in strip, the front of the culture plate is provided with a digital display screen for displaying the culture temperature and culture time, and both sides of the culture plate are provided with half-moon grooves.
[0013] Optionally, a controller and a flow control valve connected to one end of the first drainage channel are provided inside the control component, a control panel is provided on the front of the control component, the control panel includes a temperature control button for controlling the temperature controller and a flow control button for controlling the liquid inlet speed and liquid inlet amount, and an interface for liquid inlet is provided on the outer shell of the control component.
[0014] Optionally, the liquid outlet component includes a liquid outlet pipe arranged at the outer bottom of the culture bottle and a rotary switch arranged on the liquid outlet pipe, a blocking piece connected to the rotary switch is arranged inside the liquid outlet pipe, and a sealing ring is arranged on the edge of the blocking piece.
[0015] Therefore, the utility model adopts a cell culture device with drainage function of the above structure, and the beneficial effects achieved are:
[0016] 1. The utility model provides a drainage plate and a culture bottle with a drainage function, and can control the drainage speed and drainage amount of the culture solution through the control component, control the usage of the culture solution, and provide cells with more biologically similar culture conditions. In addition, through the liquid outlet tube at the bottom of the culture bottle, the situation of liquid hanging on the bottle mouth and the bottle body is avoided, thereby ensuring the sterile environment and safety issues during the cell culture process.
[0017] 2. The utility model can realize the function of automatic temperature control by setting a temperature sensor and a temperature controller, observe and adjust the culture temperature in real time on the digital display screen, realize the regulation of the microenvironment, ensure that the cells are in the most suitable growth environment, and greatly improve the cell culture efficiency.
[0018] The technical solution of the utility model is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.
[0020] Figure 1 A schematic diagram of the structure of the device provided by the utility model;
[0021] Figure 2 A schematic diagram of the structure of the guide plate provided by the utility model;
[0022] Figure 3 A schematic diagram of the device structure of the culture plate provided by the utility model;
[0023] Figure 4 A schematic diagram of the device structure of the culture vessel provided by the utility model;
[0024] Figure numerals: 1. Drainage plate; 11. Drainage groove; 12. Flexible drainage tube; 13. First drainage channel; 14. Second drainage channel; 15. Snap-on strip; 16. Switch knob; 2. Culture plate; 21. Placement area; 22. Snap-on groove; 23. Digital display screen; 24. Half-moon groove; 3. Control assembly; 31. Control panel; 32. Flow control valve; 33. Interface; 4. Culture bottle; 41. Liquid outlet tube; 42. Blocking plate; 43. Rotary switch. DETAILED DESCRIPTION
[0025] The technical solution of the utility model is further described below through the accompanying drawings and embodiments.
[0026] Example
[0027] like Figure 1-4 As shown, the utility model provides a cell culture apparatus with drainage function, comprising a controllable culture box and a plurality of culture vessels arranged inside the controllable culture box.
[0028] The controllable culture box includes a drainage plate 1, a culture plate 2 connected to the lower surface of the drainage plate 1, and a control component 3 connected to the sides of the drainage plate 1 and the culture plate 2.
[0029] like Figure 2 As shown, the drainage plate 1 is set to a square structure, and a plurality of drainage grooves 11 of cylindrical structure are evenly arranged in the middle of the drainage plate 1. A flexible drainage tube 12 is arranged inside the drainage groove 11. A drainage channel connected to the drainage groove 11 is arranged inside the drainage plate 1. A plurality of switch knobs 16 connected to the drainage channel are arranged on the upper surface of the drainage plate 1, that is, each drainage groove 11 is equipped with a switch knob 16, and the switch knob 16 is selectively opened or closed according to the number of culture bottles 4 used. Two clamping strips 15 are arranged on the lower surface of the drainage plate 1.
[0030] like Figure 2 As shown, the drainage channel includes a first drainage channel 13 and a plurality of second drainage channels 14 arranged perpendicular to the first drainage channel 13, one end of the first drainage channel 13 is connected to the control component 3, the middle and the other end of the first drainage channel 13 are connected to the second drainage channel 14, and both ends of the second drainage channel 14 are connected to the flexible drainage tube 12. After the control component 3 adjusts the liquid inlet speed and liquid inlet flow rate of the culture solution, the culture solution is transported to the flexible drainage tube 12 through the drainage channel, thereby realizing the liquid addition operation of the culture vessel.
[0031] like Figure 1 As shown, the control component 3 is provided with a controller and a flow control valve 32 connected to one end of the first drainage channel 13, and the front of the control component 3 is provided with a control panel 31, and the control panel 31 includes a temperature control button for controlling the temperature controller and a flow control button for controlling the liquid inlet speed and liquid inlet amount (controlled by the flow control valve 32), and the outer shell of the control component 3 is provided with an interface 33 for liquid inlet.
[0032] like Figure 3 As shown, the culture plate 2 is configured as a square structure with a hollow interior, and a plurality of placement areas 21 for placing the culture vessels are evenly arranged on the inner bottom of the culture plate 2, and the placement area 21 is arranged directly below the drainage groove 11, and a temperature controller and a temperature sensor are arranged in the placement area 21. The digital display screen 23 can display the real-time temperature of the cell culture in real time according to the temperature sensor, and then adjust according to the real-time temperature to ensure that the cells are in the most suitable growth environment, which greatly improves the cell culture efficiency.
[0033] like Figure 3As shown, the top of the culture plate 2 is provided with a snap-in groove 22 adapted to the snap-in strip 15, the front of the culture plate 2 is provided with a digital display screen 23 for displaying the culture temperature and culture time, and both sides of the culture plate 2 are provided with a half-moon groove 24. The drainage plate 1 and the culture plate 2 are movably connected through the snap-in strip 15 and the snap-in groove 22, and when installing or removing, the half-moon groove 24 can be buckled and directly pulled out.
[0034] like Figure 4 As shown, the culture vessel includes a culture bottle 4 and a liquid outlet assembly arranged at the outer bottom of the culture bottle 4. The liquid outlet assembly includes a liquid outlet pipe 41 arranged at the outer bottom of the culture bottle 4 and a screw switch 43 arranged on the liquid outlet pipe 41. A blocking piece 42 connected to the screw switch 43 is arranged inside the liquid outlet pipe 41, and a sealing ring is arranged at the edge of the blocking piece 42. The screw switch 43 opens the channel of the liquid outlet pipe 41 when it is rotated clockwise, and closes the channel of the liquid outlet pipe 41 when it is rotated counterclockwise.
[0035] Therefore, the utility model provides a cell culture device with a drainage function. When draining cell culture fluid, the drainage function is used to control the liquid inlet speed and liquid inlet amount, control the usage of the culture fluid, and improve the cell culture efficiency.
[0036] Finally, it should be noted that: This article uses specific examples to illustrate the principles and implementation methods of the utility model. The above examples are only used to help understand the method and core ideas of the utility model. At the same time, for those skilled in the art, according to the ideas of the utility model, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the utility model.
Claims
1. A cell culture apparatus with drainage function, characterized in that: It comprises a controllable culture box and a plurality of culture vessels arranged inside the controllable culture box; The controllable culture box comprises a drainage plate, a culture plate connected to the lower surface of the drainage plate, and a control component connected to the drainage plate and the side of the culture plate; The culture vessel comprises a culture bottle and a liquid outlet component arranged at the outer bottom of the culture bottle.
2. A cell culture apparatus with drainage function according to claim 1, characterized in that: The drainage plate is arranged in a square structure, a plurality of drainage grooves of cylindrical structure are evenly arranged in the middle of the drainage plate, a flexible drainage tube is arranged on the inner side of the drainage groove, a drainage channel connected with the drainage groove is arranged inside the drainage plate, a plurality of switch knobs connected with the drainage channel are arranged on the upper surface of the drainage plate, and two clamping strips are arranged on the lower surface of the drainage plate.
3. The cell culture apparatus with drainage function according to claim 2, characterized in that: The drainage channel includes a first drainage channel and a plurality of second drainage channels arranged perpendicularly to the first drainage channel, one end of the first drainage channel is connected to the control component, the middle part and the other end of the first drainage channel are connected to the second drainage channel, both ends of the second drainage channel are connected to the flexible drainage tube, and the switch knob is arranged at both ends of the second drainage channel.
4. The cell culture apparatus with drainage function according to claim 3, characterized in that: The culture plate is configured as a square structure with a hollow interior, and a plurality of placement areas for placing the culture vessels are evenly arranged on the inner bottom of the culture plate, the placement areas are arranged directly below the drainage groove, and a temperature controller and a temperature sensor are arranged in the placement areas; The top of the culture plate is provided with a snap-in groove matched with the snap-in strip, the front of the culture plate is provided with a digital display screen for displaying the culture temperature and culture time, and both sides of the culture plate are provided with half-moon grooves.
5. The cell culture apparatus with drainage function according to claim 4, characterized in that: A controller and a flow control valve connected to one end of the first drainage channel are arranged inside the control component, a control panel is arranged on the front of the control component, the control panel includes a temperature control button for controlling the temperature controller and a flow control button for controlling the liquid inlet speed and liquid inlet amount, and an interface for liquid inlet is arranged on the outer shell of the control component.
6. The cell culture apparatus with drainage function according to claim 5, characterized in that: The liquid outlet assembly includes a liquid outlet pipe arranged at the outer bottom of the culture bottle and a screw switch arranged on the liquid outlet pipe. A blocking piece connected to the screw switch is arranged inside the liquid outlet pipe, and a sealing ring is arranged on the edge of the blocking piece.