An apparatus for capturing and analyzing the spore density in air

Abstract

The present invention relates to the technical field of agricultural disease microorganism detection equipment, and particularly relates to an air spore density capture and analysis device, which includes an outer chassis and an inner chassis arranged inside the outer chassis. A plurality of air intake grilles for absorbing air are provided on both side walls of the outer chassis; a working platform is arranged inside the inner chassis, and a working station turntable is rotatably connected to the working platform. Spore capture units, spore sampling units, spore culture units, and spore microscopic photography units are circumferentially arranged on the edge of the working station turntable. A film covering unit and a culture solution titration unit are arranged between the spore sampling unit and the spore culture unit; the present invention sucks the outside air into the outer casing, so that the spores in the air freely adhere to the glass slide, and monitors the adhesion situation by collecting and culturing the spores on the glass slide. At the same time, the image is sent to an external controller for information analysis, and the analysis result is sent to the monitoring center in the background, so as to realize remote operation control.

Description

Technical Field

[0001] The present invention relates to the technical field of agricultural disease microorganism detection equipment, and particularly relates to an air spore density capture and analysis device. Background Art

[0002] Smart agriculture utilizes modern information technology to achieve the intelligentization, precision, and high efficiency of agricultural production; among them, pest control is an important part of smart agriculture. Relevant research shows that the occurrence of plant diseases is closely related to pathogen spores. The difference in spore concentration in the air directly affects agricultural production; and the spore detection technology in agriculture realizes the prediction and prevention of agricultural pests and diseases through the collection and detection of spores, and provides effective data analysis.

[0003] Traditional pest control methods mainly rely on manual observation and empirical judgment, which have problems such as poor timeliness and low accuracy. For this reason, devices that can automatically capture and analyze spores have been manufactured in the prior art. For example, the utility model patent with the publication number CN2191017439U discloses a pathogen spore capture and analyzer, which mainly includes a housing. A cover body is provided on the top of the housing. An air inlet is provided on the cover body. A diversion part is provided at the bottom of the air inlet. A microporous plate is installed at the bottom end of the diversion part. A vertically arranged air duct is installed in the inner cavity of the housing. A fan is installed at the bottom end of the air duct. A placement bin is provided in the middle of the inner cavity of the air duct. There is a glass slide inside the placement bin. The placement bin is fixedly connected above a heat conduction block. The heat conduction block is fixedly connected to the upper surface of a conveyor belt. The conveyor belt runs through the side wall of the air duct horizontally. A microscopic photographing module is provided in the space between the air duct and the side wall of the housing. The microscopic photographing module is located above one end of the conveyor belt. The full automation of capturing, culturing, and microscopic imaging observation of spores in the air is realized by using this device, improving work efficiency; however, when this device is actually applied in production, there are still the following defects:

[0004] (1) During the process of capturing spores, since the inhaled air contains not only spores but also other pathogen categories, and the capture device cannot effectively capture spores, and the constant temperature environment during cultivation is not suitable for all spore types, which will not only affect the growth of spores, but seriously may also lead to the death of spores;

[0005] (2) After the microscopic photographing and observation of this device, it cannot communicate and connect with an external client, so remote control cannot be achieved, that is, real-time data cannot be fed back, and manual sampling and regulation are required;

[0006] (3) When facing bad weather or power outages, this device cannot work, resulting in low work efficiency. Summary of the Invention

[0007] To solve the technical defects proposed in the above-mentioned background art, the object of the present invention is to provide an air spore density capture and analysis device, which can monitor and analyze the spore types and concentrations in the air of farmland in real time, and provide accurate pest and disease early warning and management decision-making support for smart agriculture.

[0008] To achieve the above object, the present invention adopts the following technical solutions:

[0009] An air spore density capture and analysis device, including an outer chassis and an inner chassis arranged inside the outer chassis. A plurality of air intake grilles for absorbing air are provided on both side walls of the outer chassis; a working platform is arranged inside the inner chassis, and a working station turntable is rotatably connected to the working platform. A spore capture unit, a spore sampling unit, a spore culture unit, and a spore microscopic photography unit are circumferentially arranged on the edge of the working station turntable. A film covering unit and a culture solution titration unit are arranged between the spore sampling unit and the spore culture unit; the film covering unit includes a film covering bracket, a film covering guide rod, and a coating ball. One end of the film covering guide rod is fixedly connected to the film covering bracket; the other end is movably connected to the coating ball. The coating ball is in a spherical structure and is used to coat the spore capture film onto the glass slide; a power supply mechanism is arranged on the top of the outer chassis, and the power supply mechanism is electrically connected to each unit;

[0010] It also includes a touch screen controller arranged on the surface of the inner chassis. The touch screen controller is signal-connected to an external client, and intelligently analyzes and counts the spore types and quantities in the spore images captured by the microscopic photography unit and transmits the results to the external client.

[0011] Preferably, an observation hole convenient for microscopic observation is provided on the working station turntable. A bearing block for placing a fixed glass slide is fitted in the observation hole. The bottom end of the bearing block is fixedly connected with a rotating wheel, and there is a certain height difference between the top end of the bearing block and the surface of the working station turntable, so that the glass slide is fixed in the observation hole.

[0012] Preferably, the spore capture unit includes an air inlet chamber, an air duct, and an air suction fan. One end of the air duct is connected to the air inlet chamber, and the other end extends to the working station turntable. The air suction fan is installed in the air inlet chamber. The air inlet chamber is in a funnel shape and is communicated with the air intake grille; under the action of the air suction fan, the air inlet chamber sucks air through the air intake grille and introduces it into the air duct, and the air duct introduces the air into the spore sampling unit.

[0013] Preferably, the spore sampling unit includes a sampling bracket, a storage device, and a cleaning scraper. One end of the storage device is fixed to the sampling bracket, and a storage groove for storing a glass slide is provided on the storage device. Limiting plates for preventing the glass slide from slipping out are arranged on both sides of the storage groove. One end of the cleaning scraper is fixedly connected to the sampling bracket, and a scraping strip is inserted at the other end. The scraping strip is arranged in contact with the surface of the station turntable.

[0014] Preferably, the culture solution titration unit includes a titration bracket, a titration pump, and a controller. A plurality of titration pumps are provided, and the plurality of titration pumps are respectively fixed on the titration bracket. One end of the titration pump is connected to a titration tube. The controller is electrically connected to the titration pump to control the titration pump to drip the culture solution according to the position of the glass slide.

[0015] Preferably, the spore culture unit includes a culture bracket, an incubator, and a heater. The bottom end of the incubator is of an open structure, and the incubator is fixed on the culture bracket. A culture lamp is suspended in the incubator. A dual-control temperature controller for controlling the heater is also arranged on one side of the incubator. The heater extends into the incubator.

[0016] Preferably, the spore microscopic photographing unit includes a microscopic bracket, a lifting drive assembly, a microscopic camera, and a supplementary light table. One end of the lifting drive assembly is fixed to the microscopic bracket, and the other end is slidably connected to the microscopic camera. The microscopic camera is arranged above the station turntable. The supplementary light table is arranged below the station turntable and is arranged coaxially with the microscopic camera.

[0017] Preferably, the lifting drive assembly includes a lifting slide table, a fixing plate, a slider, a connecting plate, a lead screw, and a driving motor. The lifting slide table is fixedly connected to the microscopic bracket through the fixing plate. The slider is slidably connected to the lifting slide table, and a threaded hole is provided at the axis of the slider. The lead screw passes through the threaded hole and is threadedly connected to the slider. One end of the connecting plate is fixedly connected to the slider, and the other end is fixedly connected to the microscopic camera. The driving motor is arranged at the top end of the lifting slide table, and the output end of the driving motor is in transmission connection with the lead screw.

[0018] Preferably, an electrical control box is arranged on one side of the inner chassis. The electrical control box is electrically connected to the spore capture unit, the spore sampling unit, the spore culture unit, and the spore imaging detection unit respectively to control the wind force of the spore capture unit, adjust the temperature and humidity of the spore culture unit, and control the shooting parameters of the spore imaging detection unit.

[0019] Preferably, a power supply mechanism is arranged on the top of the outer chassis. The power supply mechanism includes a solar panel and a wind power generator. A solar tracking component is connected between the solar panel and the chassis; a wind vane is arranged at the tail of the wind power generator, and a rotating shaft is arranged at the bottom of the wind power generator and is rotatably connected between the solar panels.

[0020] In summary, the beneficial effects of the present invention are as follows:

[0021] 1. The spore density capture and analysis device in the air of the present invention can monitor the spore concentration in the farmland air in real time, and timely detect the occurrence and development of pests and diseases; by sucking the external air into the inner chassis through the spore capture unit, the spore capture is not affected by the wind direction, and the pathogenic spores in the air can be captured to the greatest extent, improving the spore capture effect; at the same time, the film covering unit and the culture solution titration unit are used to effectively capture the spore types in the air onto the glass slide, and after culturing by automatically and precisely controlling the temperature required for the growth of spores through the spore culture unit, it is easier to take clear spore images through the spore microscopic photographing unit; furthermore, it is convenient to count and classify the captured spore density images.

[0022] 2. After calculation and analysis by setting the touch screen controller in the present invention, accurate spore density data can be obtained and sent to the externally connected client; thus, the occurrence of pests and diseases can be warned in advance, and the remote control of the spore capture density analysis device can be realized through the control center.

[0023] 3. By setting the power supply mechanism in the present invention, solar energy and wind energy are used together to provide power support for each unit, so that it can still work in the face of bad weather or power failure, improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a schematic structural diagram of the spore density capture and analysis device in the air of the present invention;

[0025] Figure 2 is a schematic structural diagram of each unit inside the inner chassis of the present invention;

[0026] Figure 3 is a schematic structural diagram of the work station turntable of the present invention;

[0027] Figure 4 is a schematic structural diagram of the spore sampling unit of the present invention;

[0028] Figure 5 is a schematic structural diagram of the film covering unit of the present invention;

[0029] Figure 6 is a schematic structural diagram of the culture solution titration unit of the present invention;

[0030] Figure 7 is a schematic structural diagram of the spore culture unit of the present invention;

[0031] Figure 8 is a schematic structural diagram of the spore microscopic photographing unit of the present invention.

[0032] Explanation of the reference numerals in the figures:

[0033] 1. Outer chassis; 11. Air intake grille; 12. Power supply mechanism; 121. Solar panel; 122. Wind power generator fan; 2. Inner chassis; 21. Working platform; 22. Electrical control box; 3. Workstation turntable; 31. Observation hole; 32. Carrier block; 33. Rotating wheel; 34. Collection box; 4. Spore capture unit; 41. Air inlet chamber; 42. Air duct; 43. Exhaust fan; 5. Spore sampling unit; 51. Sampling bracket; 52. Storage; 521. Storage tank; 522. Limiting plate; 53. Cleaning scraper; 531. Scraping strip; 6. Film coating unit; 61. Film coating bracket; 62. Film coating guide rod; 63. Coating ball; 7. Culture solution titration unit; 71. Titration bracket; 72. Titration pump; 73. Controller; 74. Burette; 8. Spore culture unit; 81. Culture bracket; 82. Incubator; 83. Heater; 84. Culture lamp; 85. Dual-control temperature controller; 9. Spore microscopic photography unit; 91. Microscopic bracket; 92. Lifting drive assembly; 921. Lifting slide; 922. Fixed plate; 923. Slide block; 924. Connecting plate; 925. Lead screw; 926. Driving motor; 93. Microscopic camera; 94. Fill light table; 10. Touch screen controller. Detailed implementation manners

[0034] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art belong to the scope of protection of the present invention.

[0035] Those skilled in the art should understand that in the disclosure of the present invention, the orientation or positional relationship indicated by the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the above terms should not be construed as limiting the present invention.

[0036] In addition, the terms "installed", "set up", "equipped with", "connected", and "linked" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, or there can be internal communication between two devices, components, or parts. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0037] In the description of the present invention, if there are descriptions with words such as "several", its meaning is one or more, and the meaning of multiple is two or more. Understandings such as greater than, less than, exceeding, etc. do not include the corresponding number, and understandings such as above, below, within, etc. include the corresponding number. If there are descriptions of first, second, third, etc., they are only for the purpose of distinguishing technical features and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features or implicitly indicating the sequence relationship of the indicated technical features.

[0038] The following will further elaborate on the embodiments of an apparatus for analyzing the spore density in the air according to the present invention in conjunction with the attached Figure 1-8 drawings.

[0039] An apparatus for capturing and analyzing the spore density in the air, as Figure 1 、 2 shown, includes an outer chassis 1 and an inner chassis 2 disposed inside the outer chassis 1. A plurality of intake grilles 11 for absorbing air are provided on both side walls of the outer chassis 1; a working platform 21 is disposed inside the inner chassis 2, and a station turntable 3 is rotatably connected to the working platform 21. Spore capture units 4, spore sampling units 5, spore culture units 8, and spore microscopic photography units 9 are circumferentially disposed on the edge of the station turntable 3. A film covering unit 6 and a culture solution titration unit 7 are disposed between the spore sampling unit 5 and the spore culture unit 8; after the spore capture unit 4 automatically captures the spores in the air and transfers them into the inner chassis 2, at the same time, the spore sampling unit automatically picks up and places a glass slide onto the spore sampling unit 5 to complete the spore collection process. After being cultured and processed by the spore culture unit 8, it is transferred to the spore microscopic photography unit 9 to complete the acquisition and shooting; it further includes a touch screen controller 10 disposed on the surface of the inner chassis 2. The touch screen controller 10 is signal-connected to an external client, and intelligently analyzes and counts the types and quantities of spores in the images captured by the spore microscopic photography unit 9 and transmits the results to the external client.

[0040] Specifically, the external air is drawn into the inner chassis 2 by the spore capture unit 4, so that the spore capture is not affected by the wind direction, and the pathogenic spore in the air can be captured to the greatest extent, improving the spore capture effect. At the same time, the spore sampling unit 5, the film covering unit 6 and the culture solution titration unit 7 capture the spores in the air on the glass slide, and after being cultured by the spore culture unit 8, the spores are more likely to be photographed clearly by the spore microscopic photographing unit 9. Furthermore, it is convenient to count and classify the photographed spore density images to obtain accurate spore density data, and the data is sent to the externally connected client through the touch screen controller 10, so as to be able to give an early warning of the occurrence of plant diseases and insect pests. In addition, the remote control of the spore capture density analysis device can be realized through the control center, and thus the spore concentration in the farmland air can be monitored in real time, and the development of plant diseases and insect pests can be intervened in time. At the same time, the air spore density analysis device of the present invention can be appropriately adjusted according to the actual situation of the farmland. For example, the layout and quantity of the spore sampling unit 5 can be adjusted according to the size and shape of the farmland; the appropriate spore capture liquid and the spore culture unit 8 can be selected according to the characteristics of plant diseases and insect pests of different crops. In addition, the device can also be combined with other agricultural informatization systems to form a comprehensive intelligent agricultural management platform.

[0041] In this embodiment, an electrical control box 22 is provided on one side of the inner chassis 2. The electrical control box 22 is electrically connected to the spore capture unit 4, the spore sampling unit 5, the spore culture unit 8 and the spore microscopic photographing unit 9 respectively to control the wind force of the spore capture unit 4, adjust the temperature and humidity of the spore culture unit 8, and control the shooting parameters of the spore microscopic photographing unit 9. A power supply mechanism 12 is provided on the top of the outer chassis 1. The power supply mechanism 12 includes a solar panel 121 and a wind power generating fan 122. A sun tracking component is connected between the solar panel 121 and the outer chassis 1. The sun tracking component uses a plurality of gear transmissions and worm and worm gear transmissions to realize the adjustment of the direction, and can track the irradiation direction of the sun, so as to drive the solar panel 121 to be adjusted in real time according to the irradiation angle of the sun, so that the solar panel always faces the sun to collect energy and convert it into electric energy. A wind vane is provided at the tail of the wind power generating fan 122, and a rotating shaft at the bottom of the wind power generating fan 122 is rotatably connected to the solar panel 121.

[0042] Specifically, the power supply of the electrical control box 22 comes from the power supply mechanism 12. The operation of the entire device is completed through the functions of the solar panel 121 and the wind power generation fan 122. The solar panel 121 uses the solar tracking component to complete the tracking of the sun, and can automatically adjust the tilt direction according to the irradiation direction of the sunlight, so that the solar panel 121 can collect sunlight to the greatest extent and convert it into electrical energy. The purpose of setting the wind power generation fan 122 is to generate electrical energy for the normal operation of the device when there is no sunlight or in rainy and cloudy weather. The wind vane set at the tail of the wind power generation fan 122 can automatically monitor the wind direction and automatically control the rotation direction of the wind power generation fan 122, so that it can automatically adjust according to the wind direction. The wind power generation fan 122 is rotatably installed on the solar panel 121, so that it can rotate according to the wind direction to adjust the windward direction and keep the wind power generation fan 122 in a power generation state at all times.

[0043] In this embodiment, as Figure 3 shown, an observation hole 31 convenient for microscopic observation is formed in the station turntable 3. A bearing block 32 for placing a fixed glass slide is fitted in the observation hole 31. A rotating wheel 33 is fixedly connected to the bottom end of the bearing block 32, and there is a certain height difference between the top end of the bearing block 32 and the surface of the station turntable 3, so that the glass slide is fixed in the observation hole 31. The purpose of this setting is to enable the glass slides in the spore sampling unit 5 to fall into the observation hole 31 one by one, and the entire blanking process is automatically controlled, effectively avoiding excessive accumulation of glass slides in the observation hole 31 and causing waste.

[0044] Specifically, the bottom end of the station turntable 3 is connected to a driving mechanism. The driving mechanism includes a gear turntable, a driving motor and a driving gear. The driving gear is fixed to the output shaft end of the driving motor, and the driving gear is located in the gear turntable and meshed with the gear turntable to drive the station turntable 3 to rotate. A plurality of through holes are circumferentially distributed on the working platform 21 at a position opposite to the station turntable 3. A collection box 34 is arranged below one of the through holes. The collection box 34 is used to collect the glass slides after shooting and detection.

[0045] In this embodiment, the spore capture unit 4 includes an air inlet cavity 41, an air duct 42 and a suction fan 43. One end of the air duct 42 is connected to the air inlet cavity 41, and the other end extends to the station turntable 3. The suction fan 43 is installed in the air inlet cavity 41. The air inlet cavity 41 is in a funnel shape and is communicated with the air intake grille 11. Under the action of the suction fan 43, the air inlet cavity 41 sucks air through the air intake grille 11 and introduces it into the air duct 42, and the air duct 42 introduces the air into the spore sampling unit 5.

[0046] Specifically, when the spore capture unit 4 extracts external air, the intake grilles 11 provided on both sides of the outer chassis 1 can further increase the air extraction area, and the structure of the grilles can also effectively prevent rainwater from entering the outer chassis 1. The suction fan 43 is installed in the air inlet chamber 41, and the air inlet chamber 41 is located at one end of the outer chassis 1 close to the intake grille 11. The suction fan 43 sucks the air containing spores into the air inlet chamber 41 and conveys it to the inner chassis 2 through the air duct 42. The purpose of setting the suction fan 43 is to capture the spores in the air even when there is no wind. At the same time, the suction fan 43 can be connected to an external client to achieve remote control.

[0047] In this embodiment, as Figure 4 shown, the spore sampling unit 5 includes a sampling bracket 51, a storage 52, and a cleaning scraper 53. One end of the storage 52 is fixed on the sampling bracket 51, and a storage groove 521 for storing glass slides is provided on the storage 52. Limiting plates 522 for preventing the glass slides from sliding out are provided on both sides of the storage groove 521. One end of the cleaning scraper 53 is fixedly connected to the sampling bracket 51, and a scraping strip 531 is inserted at the other end. The scraping strip 531 is arranged in contact with the surface of the working station turntable 3.

[0048] Specifically, while the suction fan 43 extracts external air, the spore sampling bracket 51 starts to drop the glass slides stored on the storage 52 into the observation holes 31, and as the working station turntable 3 rotates, the glass slides are transferred to the air outlet of the air duct 42. Among them, the spores are pressed onto the glass slides under the action of the film coating unit 6, which facilitates the subsequent observation of the spore species. During the rotation of the working station turntable 3, the glass slides on the spore sampling bracket 51 naturally fall into the observation holes 31 of the working station turntable 3 under the action of gravity. When the observation holes 31 on the working station turntable 3 leave the position of the storage 52, since there are no observation holes 31 on the other parts of the working station turntable 3, the glass slides can only fall into the observation holes 31 one by one. Then, after the glass slides complete the shooting and detection, they are scraped off from the observation holes 31 by the scraping strip 531 under the action of the cleaning scraper 53 and collected in the collection box 34.

[0049] In this embodiment, as Figure 5 shown, the film coating unit 6 includes a film coating bracket 61, a film coating guide rod 62, and a coating ball 63. One end of the film coating guide rod 62 is fixedly connected to the film coating bracket 61, and the other end is movably connected to the coating ball 63. The coating ball 63 has a spherical structure and is used to coat the spore capture film onto the glass slide. After the spores are extracted into the inner chassis 2, in order to capture as many spores as possible on the glass slide, the coating ball 63 is used to coat the spore capture film on the glass slide so that a large number of spores can be quickly fixed on the glass slide. Among them, the coating ball 63 accurately fits the glass slides on the working station turntable 3 under the drive of the film coating guide rod 62.

[0050] In this embodiment, as Figure 6 , 7 shown, the culture medium titration unit 7 includes a titration bracket 71, a titration pump 72 and a controller 73. A plurality of titration pumps 72 are provided. The plurality of titration pumps 72 are respectively fixed on the titration bracket 71, and one end of the titration pump 72 is connected with a titration tube 74. The controller 73 is electrically connected with the titration pump 72 to control the titration pump 72 to drip the culture medium according to the position of the slide; after the slide is dripped with the culture medium, it will enter the spore culture unit 8 for growth culture for a certain period of time. Among them, the spore culture unit 8 includes a culture bracket 81, a culture box 82 and a heater 83. The bottom end of the culture box 82 is of an open structure, and the culture box 82 is fixed on the culture bracket 81. A culture lamp 84 is suspended in the culture box 82. A dual-control temperature controller 85 for controlling the heater 83 is also provided on one side of the culture box 82. The heater 83 extends into the culture box 82.

[0051] In order to make the spore photograph image clearer, so as to facilitate more accurate judgment of the spore species. After the spores are captured on the slide, the culture medium is dripped onto the carrier slide by the titration pump 72 and moved into the culture box 82 through the station turntable 3. The purpose of adding the culture medium to culture the spores is to provide a suitable growth environment for the spores so that they can grow and distinguish species quickly. Among them, to ensure a suitable growth environment, the culture lamp 84 provided in the culture box 82 can accelerate its growth rate, and at the same time, the heater 83 and the dual-control temperature controller 85 are used to accurately control the temperature in the culture box 82 to provide a suitable temperature for the growth of the spores.

[0052] In this embodiment, as Figure 8 shown, the spore microphotographing unit 9 includes a microscope bracket 91, a lifting drive assembly 92, a microscope camera 93 and a supplementary light table 94. One end of the lifting drive assembly 92 is fixed on the microscope bracket 91, and the other end is slidably connected with the microscope camera 93. The microscope camera 93 is arranged above the station turntable 3, and the supplementary light table 94 is arranged below the station turntable 3 and is arranged coaxially with the microscope camera 93; among them, the lifting drive assembly 92 includes a lifting slide 921, a fixing plate 922, a slider 923, a connecting plate 924, a lead screw 925 and a driving motor 926. The lifting slide 921 is fixedly connected with the microscope bracket 91 through the fixing plate 922. The slider 923 is slidably connected to the lifting slide 921, and a threaded hole is opened at the axis of the slider 923. The lead screw 925 passes through the threaded hole and is threadedly connected with the slider 923. One end of the connecting plate 924 is fixedly connected with the slider 923, and the other end is fixedly connected with the microscope camera 93. The driving motor 926 is arranged at the top end of the lifting slide 921, and the output end of the driving motor 926 is in transmission connection with the lead screw 925, so that the height of the microscope camera 93 can be adjusted according to the detection requirements.

[0053] It is worth mentioning that the microscopic camera 93 uses a digital microscope with an automatic focusing function, and at the same time, the supplementary light table 94 uses a ring-shaped supplementary light source that automatically adjusts the light according to the environment. During the shooting process, the digital microscope can automatically change the magnification of the objective lens according to the shooting requirements to more intuitively and clearly observe the density distribution of the spore species.

[0054] The working principle of the present invention:

[0055] When starting to work, when the station turntable 3 rotates counterclockwise to the lower part of the spore sampling unit 5, the glass slide in the spore sampling unit 5 is automatically fed into the observation hole 31. At the same time, the spore capture unit 4 sucks the outside air into the inner chassis 2 through the suction fan 43 and partially falls onto the glass slide. When the station turntable 3 rotates to the lower part of the film coating unit 6, the spore capture film is coated onto the glass slide by the coating ball 63, so that a large number of spores are captured on the glass slide. The station turntable 3 continues to rotate, and the nutrient solution titration unit 7 drips the nutrient solution for culturing spores onto the glass slide so that it can obtain the required nutrients to promote its growth. Immediately afterwards, the station turntable 3 continues to rotate, so that the glass slide is transferred to the spore culture unit 8 for constant temperature culture, providing a suitable growth environment for the spores, making them clearer during the imaging process of the spore microscopic shooting unit 9. Finally, the glass slide after shooting falls into the collection box 34 under the action of the cleaning scraper 53. Among them, the touch screen controller 10 will automatically analyze the shooting results, automatically judge according to the species density of the spores, and at the same time synchronously transmit the processing results to the external client to achieve remote control; the entire spore capture density analysis device is regularly detected according to the preset time period to ensure the accuracy of the test data.

[0056] The embodiments of the present specific implementation manners are all preferred embodiments of the present application, and do not limit the protection scope of the present application accordingly. The same components are denoted by the same reference numerals. Therefore, all equivalent changes made according to the structure, shape, and principle of the present application should be covered within the protection scope of the present application.

Claims

1. A device for capturing and analyzing spore density in air, comprising an outer case and an inner case arranged inside the outer case, characterized in that: A plurality of air intake grilles for absorbing air are provided on both side walls of the outer chassis; a working platform is provided inside the inner chassis, on which a work station turntable is rotatably connected, a spore capturing unit, a spore sampling unit, a spore culture unit and a spore microscopic shooting unit are circumferentially arranged on the edge of the work station turntable, and a film coating unit and a culture liquid titration unit are arranged between the spore sampling unit and the spore culture unit; the film coating unit comprises a film coating bracket, a film coating guide rod and a film coating ball, one end of the film coating guide rod is fixedly connected to the film coating bracket; the other end is movably connected to the film coating ball, and the film coating ball is a spherical structure, which is used to coat the spore capturing film on the glass slide; a power supply mechanism is provided on the top of the outer chassis, and the power supply mechanism is electrically connected to each unit respectively; the power supply mechanism comprises a solar panel and a wind power fan, and a sun tracking assembly is connected between the solar panel and the chassis; a wind vane is provided at the tail of the wind power fan, and a rotating shaft is provided at the bottom of the wind power fan to be rotatably connected between the solar panels; The culture solution titration unit comprises a titration support, a titration pump and a controller, wherein a plurality of titration pumps are provided, the plurality of titration pumps are respectively fixed on the titration support, and a burette is connected to one end of the titration pump, and the controller is electrically connected to the titration pump to control the titration pump to drip the culture solution according to the position of the slide glass; The spore culture unit comprises a culture support, a culture box and a heater. The bottom end of the culture box is an open structure, and the culture box is fixed on the culture support. A culture lamp is suspended in the culture box. A double-control temperature control meter for controlling the heater is also arranged on one side of the culture box. The heater extends into the culture box. It also includes a touch screen controller arranged on the surface of the inner chassis, which is connected to the external client signal, and intelligently analyzes and counts the types and quantities of spores in the spore image taken by the microscopic shooting unit and transmits the results to the external client.

2. The device for capturing and analyzing spore density in air according to claim 1, characterized in that: An observation hole convenient for microscopic observation is provided on the work station turntable, a carrying block for placing and fixing a glass slide is embedded in the observation hole, a rotating wheel is fixedly connected to the bottom end of the carrying block, and there is a certain height difference between the top end of the carrying block and the surface of the work station turntable so that the glass slide can be fixed in the observation hole.

3. The device for capturing and analyzing spore density in air according to claim 2, characterized in that: The spore capturing unit comprises an air inlet chamber, an air duct and a suction fan, one end of the air duct is connected to the air inlet chamber, and the other end extends to the workstation turntable, the suction fan is installed in the air inlet chamber, the air inlet chamber is funnel-shaped, and the air inlet chamber is connected to the air inlet grille; under the action of the suction fan, the air inlet chamber draws air through the air inlet grille and introduces it into the air duct, and the air is introduced into the spore sampling unit by the air duct.

4. The device for capturing and analyzing spore density in air according to claim 3, characterized in that: The spore sampling unit comprises a sampling bracket, a storage container and a cleaning scraper. One end of the storage container is fixed on the sampling bracket, and a storage slot for storing glass slides is provided on the storage container. Limiting plates are provided on both sides of the storage slot to prevent the glass slides from slipping out. One end of the cleaning scraper is fixedly connected to the sampling bracket, and a scraping strip is inserted at the other end. The scraping strip is arranged to fit the surface of the workstation turntable.

5. The device for capturing and analyzing spore density in air according to claim 4, characterized in that: The spore microscopic shooting unit includes a microscopic support, a lifting drive component, a microscopic camera and a fill light stage. One end of the lifting drive component is fixed on the microscopic support, and the other end is slidably connected to the microscopic camera. The microscopic camera is arranged above the workstation turntable, and the fill light stage is arranged below the workstation turntable, and the fill light stage and the microscopic camera are arranged on the same axis.

6. The device for capturing and analyzing spore density in air according to claim 5, characterized in that: The lifting drive assembly includes a lifting slide, a fixed plate, a slider, a connecting plate, a screw and a driving motor. The lifting slide is fixedly connected to the microscope bracket through the fixed plate. The slider is slidably connected to the lifting slide, and a threaded hole is opened on the axis of the slider. The screw passes through the threaded hole and is threadedly connected to the slider. One end of the connecting plate is fixedly connected to the slider, and the other end is fixedly connected to the microscope camera. The driving motor is arranged at the top of the lifting slide, and the output end of the driving motor is transmission-connected to the screw.

7. The device for capturing and analyzing spore density in air according to claim 1, characterized in that: An electrical control box is provided on one side of the inner chassis, and the electrical control box is electrically connected to the spore capturing unit, the spore sampling unit, the spore cultivating unit and the spore video detecting unit, respectively, so as to control the wind force of the spore capturing unit, adjust the temperature and humidity of the spore cultivating unit, and control the shooting parameters of the spore video detecting unit.

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