Multi-channel online spectrum detection device

Abstract

The utility model discloses a multichannel on-line spectrum detection device which comprises a base and a detection block arranged on the base, a hollow negative pressure bin is arranged at the lower end of the detection block, a plurality of longitudinally extending flow cells are formed at the upper part of the detection block, and each flow cell is communicated with the negative pressure bin through an electromagnetic valve; the spectrum detection device further comprises a moving frame, a translation driving mechanism, a spectrograph and a collimating mirror; the collimating mirror and the spectrograph are mounted on the moving frame; the translation driving mechanism drives the moving frame to move along the length direction of the detection block; the spectrum detection device further comprises a first vacuum pump and a second vacuum pump, an upper through hole, a middle through hole and a lower through hole are sequentially formed in the wall of the negative pressure bin from top to bottom, the upper through hole is communicated with the first vacuum pump, a liquid level sensor is installed in the middle through hole, and the lower through hole is communicated with the second vacuum pump. The device can be used in cooperation with equipment such as a synthesizer, online real-time detection of a reagent to be detected is achieved, and qualitative detection and quantitative detection with relative precision can be achieved.

Description

Technical Field

[0001] The utility model belongs to the technical field of gene synthesis supporting equipment, and particularly relates to a multi-channel online spectrum detection device. Background Art

[0002] The current primer synthesis equipment cannot know the status of the synthetic product (or reagent to be tested) in real time, so the problems in the synthesis process can only be discovered in subsequent tests, resulting in a waste of reagents to be tested and time. In addition, the existing spectrometer is large in size and high in cost, and is not suitable for online detection. Other testing technologies are also not suitable for testing this micro-synthesis process.

[0003] Therefore, according to the above requirements, we aim to study a multi-channel online spectral detection device that can realize online detection of the reagents to be tested. Utility Model Content

[0004] The main technical problem solved by the utility model is to provide a multi-channel online spectrum detection device, which can be used in conjunction with equipment such as a synthesizer to achieve online real-time detection of reagents to be detected, and can achieve qualitative detection and quantitative detection with relative accuracy.

[0005] In order to solve the above technical problems, a technical solution adopted by the utility model is as follows: the utility model provides a multi-channel online spectrum detection device, comprising a base and a detection block arranged on the base, the lower end of the detection block is a hollow negative pressure chamber, and the upper part forms a plurality of longitudinally extending flow cells, all of the flow cells are independent of each other and are not connected, and each flow cell is connected to the negative pressure chamber through an electromagnetic valve;

[0006] The two sides of the circulation pool are transparent windows; the spectrum detection device also includes a moving frame, a translation driving mechanism, a spectrometer and a collimating mirror, the collimating mirror and the spectrometer are installed on the moving frame, and are respectively located on the two sides of the circulation pool and aligned with the transparent window; the moving frame is driven by the translation driving mechanism to move along the length direction of the detection block;

[0007] The spectral detection device also includes two vacuum pumps, which are defined as a first vacuum pump and a second vacuum pump respectively. An upper through hole, a middle through hole and a lower through hole are provided on the wall of the negative pressure chamber from top to bottom. The upper through hole is connected to the first vacuum pump, the middle through hole is installed with a liquid level sensor, and the lower through hole is connected to the second vacuum pump.

[0008] Furthermore, the translation driving mechanism includes a driving motor, a screw rod and a nut, the moving frame is fixedly mounted on the nut, the nut is sleeved on the screw rod, and the driving motor drives the screw rod to rotate to drive the nut to translate.

[0009] Furthermore, the movable frame and the detection block are slidably connected via a linear guide rail.

[0010] Furthermore, the light source of the spectrometer is a deuterium lamp.

[0011] Furthermore, the vacuum pump is a negative pressure diaphragm pump.

[0012] Furthermore, the upper end of the circulation pool is an inlet, and the circulation pool is a channel that runs through from top to bottom.

[0013] The beneficial effects of the utility model are:

[0014] The utility model includes a base and a detection block arranged on the base, the lower end of the detection block is a hollow negative pressure chamber, and the upper part forms a plurality of longitudinally extending flow cells, each of which is connected to the negative pressure chamber through an electromagnetic valve; the spectral detection device also includes a mobile frame, a translation drive mechanism, a spectrometer and a collimator, and the collimator and the spectrometer are installed on the mobile frame; the external pipeline containing the reagent to be tested is connected to the upper end of the flow cell, and the vacuum negative pressure of the vacuum pump causes the reagent to be tested to flow into the corresponding flow cell, and the mobile frame is driven by the translation drive mechanism to move along the length direction of the detection block to align with the corresponding flow cell, and then the reagent to be tested is tested; therefore, the utility model can be used as an independent individual to cooperate with various existing synthesizers to monitor the state of the synthesized product online, and at the same time, this device can also be used as a sampling unit to expand to similar needs, with strong versatility; the test is accurate, UL-level online detection is achieved, and in-depth development and customization can be achieved to meet more needs.

[0015] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the utility model in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is one of the structural schematic diagrams of the utility model;

[0017] Figure 2 This is the second structural diagram of the utility model;

[0018] Figure 3 This is the third structural diagram of the utility model;

[0019] Figure 4 It is a cross-sectional view of the utility model (cut along a direction perpendicular to the width of the detection block);

[0020] The reference numerals are as follows:

[0021] Base 1, detection block 2, negative pressure chamber 21, upper through hole 211, middle through hole 212, lower through hole 213, circulation pool 22, inlet 221, transparent window 23, solenoid valve 3, moving frame 4, translation drive mechanism 5, drive motor 51, screw rod 52, nut 53, linear guide rail 54, spectrometer 6, collimating mirror 7, first vacuum pump 8, second vacuum pump 9. DETAILED DESCRIPTION

[0022] The following is a specific embodiment of the present invention, and those skilled in the art can easily understand the advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented in other different ways, that is, different modifications and changes can be made without departing from the scope disclosed by the present invention.

[0023] Embodiment: A multi-channel online spectrum detection device, such as Figures 1 to 4 As shown, it comprises a base 1 and a detection block 2 arranged on the base, the lower end of the detection block is a hollow negative pressure chamber 21, and a plurality of longitudinally extending flow cells 22 are formed on the upper part, all of the flow cells are independent of each other and are not connected, and each flow cell is connected to the negative pressure chamber through an electromagnetic valve 3;

[0024] The two sides of the circulation pool are transparent windows 23; the spectrum detection device also includes a moving frame 4, a translation driving mechanism 5, a spectrometer 6 and a collimator 7, the collimator and the spectrometer are installed on the moving frame, and are respectively located on the two sides of the circulation pool and aligned with the transparent window; the moving frame is driven by the translation driving mechanism to move along the length direction of the detection block;

[0025] The spectral detection device also includes two vacuum pumps, which are defined as a first vacuum pump 8 and a second vacuum pump 9 respectively. An upper through hole 211, a middle through hole 212 and a lower through hole 213 are provided on the wall of the negative pressure chamber from top to bottom. The upper through hole is connected to the first vacuum pump, the middle through hole is installed with a liquid level sensor, and the lower through hole is connected to the second vacuum pump.

[0026] In this embodiment, the translation driving mechanism 5 includes a driving motor 51, a screw rod 52 and a nut 53. The moving frame is fixedly mounted on the nut. The nut is sleeved on the screw rod. The driving motor drives the screw rod to rotate and drives the nut to translate.

[0027] In this embodiment, the moving frame and the detection block are slidably connected via a linear guide rail 54. In this embodiment, two linear guide rails are provided, which are respectively located on the side surface and the bottom surface of the detection block.

[0028] In this embodiment, the light source of the spectrometer is a deuterium lamp, but it is not limited thereto, and any light source that meets the needs of the spectrometer can be used.

[0029] The vacuum pump is a negative pressure diaphragm pump, but is not limited thereto, other types of vacuum pumps are also acceptable.

[0030] In this embodiment, the upper end of the circulation pool is the inlet 221, and the circulation pool is a channel that passes through from top to bottom.

[0031] The working principle and working process of the utility model are as follows:

[0032] The synthesized liquid will enter one of the twelve channels (the twelve channels are taken as an example in the embodiment) from the inlet according to the time sequence through the external capillary. The host computer (referring to the control computer connected to the device) will group the sequence of the above reagents to be tested and coordinate the time sequence;

[0033] When the first vacuum pump is started, a stable negative pressure will be formed in the negative pressure chamber. By controlling the solenoid valve, we can accurately control the time point and state of the reagent to be tested in the capillary entering the circulation pool; the drive motor will cooperate with the mobile frame to drive the collimator to stop at the required position at the appropriate time. The spectrometer scans a set of data at high speed to measure the absorbance of a specific wavelength in practice. The host computer calculates the synthesis effect at that time. And synchronizes it to the synthesis unit (referring to the synthesizer) in real time;

[0034] Since the spectrometer can observe a wide absorbance range with high accuracy, in addition to measuring the absorbance of the product, we can also evaluate the by-products and other situations, making the synthesis process closed-loop and controllable.

[0035] After the detection, the first vacuum pump and the solenoid valve cooperate to allow the reagent to enter the negative pressure chamber. When the reagent in the negative pressure chamber reaches the height detected by the liquid level sensor, the sensor determines that the reagent is pumped out of the negative pressure chamber through the second vacuum pump to discharge the waste liquid.

[0036] During the reagent testing process, the outside of the device needs a light shielding cover to shield interference.

[0037] The spectrometer is time-multiplexed. In order to solve the problem of concentricity and stability of the motion-synchronized spectrometer optical path alignment, a translation drive mechanism is developed. The precise position movement of the spectrometer is achieved through the cooperation of the drive motor and the lead screw.

[0038] In order to obtain relatively accurate control of the liquid in the capillary, the double-pump negative pressure method is used, and the time redundancy is used to separate the gas phase adsorption (the first vacuum pump is used for the reagent to be tested to enter the circulation pool) and the liquid phase adsorption (the second vacuum pump is used for waste discharge) states to achieve pressure stability. The liquid flow process is precisely controlled.

[0039] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure made using the contents of the specification and drawings of the present invention, or directly or indirectly used in other related technical fields, is also included in the patent protection scope of the present invention.

Claims

1. A multi-channel online spectrum detection device, characterized in that: It comprises a base (1) and a detection block (2) arranged on the base, the lower end of the detection block is a hollow negative pressure chamber (21), and the upper part forms a plurality of longitudinally extending flow cells (22), all of the flow cells are independent of each other and are not connected, and each flow cell is connected to the negative pressure chamber via an electromagnetic valve (3); The two sides of the circulation pool are transparent windows (23); the spectrum detection device also includes a moving frame (4), a translation drive mechanism (5), a spectrometer (6) and a collimator (7); the collimator and the spectrometer are mounted on the moving frame and are respectively located on the two sides of the circulation pool and aligned with the transparent windows; the moving frame is driven by the translation drive mechanism to move along the length direction of the detection block; The spectral detection device also includes two vacuum pumps, which are defined as a first vacuum pump (8) and a second vacuum pump (9). An upper through hole (211), a middle through hole (212) and a lower through hole (213) are provided on the wall of the negative pressure chamber from top to bottom. The upper through hole is connected to the first vacuum pump, the middle through hole is installed with a liquid level sensor, and the lower through hole is connected to the second vacuum pump.

2. The multi-channel online spectrum detection device according to claim 1, characterized in that: The translation drive mechanism (5) comprises a drive motor (51), a screw rod (52) and a nut (53); the moving frame is fixedly mounted on the nut; the nut is sleeved on the screw rod; the drive motor drives the screw rod to rotate and drives the nut to translate.

3. The multi-channel online spectrum detection device according to claim 1, characterized in that: The moving frame and the detection block are slidably connected via a linear guide rail (54).

4. The multi-channel online spectrum detection device according to claim 1, characterized in that: The light source of the spectrometer is a deuterium lamp.

5. The multi-channel online spectrum detection device according to claim 1, characterized in that: The vacuum pump is a negative pressure diaphragm pump.

6. The multi-channel online spectrum detection device according to claim 1, characterized in that: The upper end of the circulation pool is an inlet (221), and the circulation pool is a channel that runs through from top to bottom.

Images