Sheep embryo freezing device

Abstract

The utility model relates to a sheep embryo freezing device, which comprises a box body, a bracket, a box cover and freezing tubes, the bracket is arranged in the box body, sunken grooves are uniformly distributed on the bracket, and the freezing tubes are arranged in the grooves; a box cover is arranged at the top of the box body; a cavity between the bracket and the box body is filled with a freezing medium; and the bracket is also provided with a liquid nitrogen supplementing device and a temperature detection probe. On the basis of a medium cryopreservation box, a device for detecting the temperature of the cryopreservation box and a corresponding device for supplementing liquid nitrogen to a medium to accelerate cooling of the medium are additionally arranged, and by monitoring the temperature of the cryopreservation box, the medium in the cryopreservation box is accelerated to cool in a short time in a liquid nitrogen supplementing mode when the temperature is reduced to a set low temperature; therefore, the retention time of the ice crystals in an ice crystal generating zone is reduced, and the generation of the ice crystals is reduced, namely the mechanical damage of the ice crystals to embryonic cells is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of embryo cryopreservation, in particular to a sheep embryo freezing device. Background Art

[0002] Embryo cryopreservation technology refers to the biological freezing and storage of fertilized eggs or early embryos so that they can be thawed and transplanted at any time, realizing the storage and utilization of embryos. It has brought changes to the protection of livestock germplasm resources and has become an effective method to preserve and inherit livestock's excellent genetic traits.

[0003] The embryo freezing process of sheep generally requires cooling the embryos according to a gradient to a certain degree and then freezing them before placing them in liquid nitrogen for long-term storage. The cooling speed must be controlled during the cooling process of the embryos. If the cooling is too slow, the water in the cells will fully flow out, increasing the solute concentration, and further denaturing the lipoprotein complexes of the cell membrane and some other functional proteins in the cytoplasm, causing irreversible damage to the cells and producing a solute effect. If the cooling is too fast, the water inside the cells will not have time to overflow to the outside of the cells and will directly form ice crystals inside the cells, causing fatal mechanical damage to the cells and producing a mechanical damage effect.

[0004] Therefore, at present, cryopreservation boxes are generally used to achieve programmed cooling of embryos. The problem of slow cooling can be solved by filling the cryopreservation boxes with corresponding media according to actual needs. The formation of ice crystals generally varies according to the water content, and the maximum ice crystal formation zone is also different, generally around -1℃~-5℃. Therefore, in order to avoid the formation of ice crystals, it is generally necessary to keep the embryo in the maximum ice crystal formation zone as short as possible. However, the current cryopreservation boxes cannot achieve the effect of accurately controlling the residence time. Summary of the invention

[0005] The utility model discloses a sheep embryo freezing device, which is based on a medium freezing box, is additionally provided with a device for detecting the temperature of the freezing box and a corresponding device for supplementing the medium with liquid nitrogen to accelerate the cooling thereof. By monitoring the temperature of the freezing box, when the temperature is lowered to a set low temperature, the medium in the freezing box is supplemented with liquid nitrogen to accelerate the cooling thereof in a short time, thereby reducing the residence time of the medium in the ice crystal formation zone, reducing the formation of ice crystals, and thus reducing the mechanical damage of ice crystals to embryo cells.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A sheep embryo freezing device comprises a box body, a bracket, a box cover and a cryopreservation tube; the box body is provided with a bracket, the bracket is provided with evenly distributed sunken grooves, and the cryopreservation tube is arranged in the grooves; the top of the box body is provided with a box cover; the cavity between the bracket and the box body is filled with a freezing medium; the bracket is also provided with a liquid nitrogen supplement device and a temperature detection probe.

[0008] The temperature detection probe is arranged at the lower part of the bracket, and the liquid nitrogen replenishing device includes a liquid nitrogen storage tank and a solenoid valve; the bottom of the liquid nitrogen storage tank is connected to the lower part of the bracket, and the solenoid valve is arranged at the bottom of the liquid nitrogen storage tank; the liquid nitrogen replenishing device also includes a control board, and the control board controls the opening and closing of the solenoid valve according to the temperature information detected by the temperature detection probe.

[0009] Furthermore, a plurality of groups of annular liquid nitrogen pipelines are provided at the lower part of the bracket, and the liquid nitrogen pipelines are evenly distributed under the bracket; and the centers of the liquid nitrogen pipelines are connected to the lower part of the liquid nitrogen storage tank.

[0010] Furthermore, a shell is provided at the center above the bracket, the liquid nitrogen storage tank is arranged in the shell, the top of the liquid nitrogen storage tank and the shell are an integrated structure, and a filling port for filling liquid nitrogen is provided at a position on the top of the shell corresponding to the liquid nitrogen storage tank, and a movably pluggable sealing plug is provided at the filling port.

[0011] Furthermore, a battery pack for supplying power to the control panel, the solenoid valve and the temperature detection probe is also provided in the housing.

[0012] Furthermore, the shell is a double-layer structure, and evenly distributed electric heating wires are provided in the gap in the middle of the double-layer shell, and the electric heating wires are powered by a battery pack.

[0013] Furthermore, the box cover is an annular structure, and when the box cover is buckled on top of the box body, the shell passes through the center of the box cover.

[0014] The utility model adopts the above technical solution to achieve the following beneficial effects:

[0015] This device adds a liquid nitrogen supplementary cooling system on the basis of the traditional cryopreservation box. By monitoring the temperature of the cryopreservation medium in the cryopreservation box, when the corresponding set temperature is reached, the cryopreservation medium is supplemented with liquid nitrogen to cool down, thereby reducing its residence time in the ice crystal formation zone, thereby reducing the formation of ice crystals and reducing the mechanical damage of ice crystals to cells.

[0016] In addition, the shell structure protects the battery pack and the liquid nitrogen replenishing device to prevent them from stopping working under low temperature conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the utility model.

[0018] Figure 2 This is a disassembly diagram of the utility model.

[0019] Figure 3 A schematic diagram of the bottom of the bracket. DETAILED DESCRIPTION

[0020] The present invention is further described below in conjunction with the accompanying drawings and embodiments. It should be clear that the following embodiments are only one or more manifestations of the present invention and are not intended to be a complete limitation on the invention contents of this application. Any improvements known to those skilled in the art made on the basis of the invention contents or technical solutions recorded in this application should fall within the scope of protection claimed in this application.

[0021] like Figure 1-Figure 3 The sheep embryo freezing device shown in the figure comprises a box body 1, a bracket 2, a box cover 3 and a cryopreservation tube 4; the box body 1 is provided with a bracket 2, the bracket 2 is provided with evenly distributed sunken grooves 201, and the cryopreservation tube 4 is arranged in the grooves 201; the top of the box body 1 is provided with a box cover 3; the cavity between the bracket 2 and the box body 1 is filled with a freezing medium 5; the bracket 2 is also provided with a liquid nitrogen replenishing device 202 and a temperature detection probe 203.

[0022] The temperature detection probe 203 is arranged at the lower part of the bracket 2, and the liquid nitrogen replenishing device 202 includes a liquid nitrogen storage tank 2021 and a solenoid valve 2022; the bottom of the liquid nitrogen storage tank 2021 is connected to the lower part of the bracket 2, and the solenoid valve 2022 is arranged at the bottom of the liquid nitrogen storage tank 2021; the liquid nitrogen replenishing device 202 also includes a control board 2023, and the control board 2023 controls the opening and closing of the solenoid valve 2022 according to the temperature information detected by the temperature detection probe 203.

[0023] Furthermore, a plurality of groups of annular liquid nitrogen pipes 204 are provided at the lower part of the bracket 2 , and the liquid nitrogen pipes 204 are evenly distributed under the bracket 2 ; and the center of the liquid nitrogen pipes 204 is connected to the lower part of the liquid nitrogen storage tank 2021 .

[0024] Furthermore, a shell 205 is provided at the center above the bracket 2, and the liquid nitrogen storage tank 2021 is provided in the shell 205. The top of the liquid nitrogen storage tank 2021 and the shell 205 are an integrated structure, and a filling port 2051 for filling liquid nitrogen is provided at a position corresponding to the liquid nitrogen storage tank 2021 on the top of the shell 205, and a movable plug-in sealing plug 2052 is provided at the filling port 2051.

[0025] Furthermore, a battery pack 206 for supplying power to the control board 2023 , the solenoid valve 2022 and the temperature detection probe 203 is also disposed in the housing 205 .

[0026] Furthermore, the shell 205 is a double-layer structure, and evenly distributed electric heating wires 207 are provided in the gap in the middle of the double-layer shell 205 . The electric heating wires 207 are powered by the battery pack 206 .

[0027] Furthermore, the box cover 3 is an annular structure. When the box cover 3 is buckled on the box body 1 , the shell 205 passes through the center of the box cover 3 .

[0028] When the device is used, a freezing medium such as isopropyl ketone is filled into the box body, and then a bracket is installed in the box body, and the cryopreservation tube containing the embryo is placed in the groove on the bracket, and then the box cover is closed and placed in a freezing environment.

[0029] By setting the control panel, when the temperature drops to the ice crystal formation zone, the solenoid valve is controlled to open and liquid nitrogen is injected into the liquid nitrogen pipeline, thereby achieving the effect of short-term rapid cooling, reducing its residence time in the ice crystal formation zone, and further achieving the effect of reducing the generation of ice crystals.

[0030] Since the entire device is used at low temperatures, in order to avoid the situation where the battery pack cannot operate at low temperatures, a double-layer shell structure is set up to effectively protect the internal battery pack, control panel, and liquid nitrogen tank to prevent low temperatures from affecting their operation.

Claims

1. A sheep embryo freezing device, comprising a box body, a bracket, a box cover and a cryopreservation tube, characterized in that: A bracket is arranged in the box body, and evenly distributed sunken grooves are arranged on the bracket, and the cryopreservation tubes are arranged in the grooves; a box cover is arranged on the top of the box body; a freezing medium is filled in the cavity between the bracket and the box body; and a liquid nitrogen replenishing device and a temperature detection probe are also arranged on the bracket.

2. A sheep embryo freezing device according to claim 1, characterized in that: The temperature detection probe is arranged at the lower part of the bracket, and the liquid nitrogen replenishing device includes a liquid nitrogen storage tank and a solenoid valve; the bottom of the liquid nitrogen storage tank is connected to the lower part of the bracket, and the solenoid valve is arranged at the bottom of the liquid nitrogen storage tank; the liquid nitrogen replenishing device also includes a control board, and the control board controls the opening and closing of the solenoid valve according to the temperature information detected by the temperature detection probe.

3. A sheep embryo freezing device according to claim 2, characterized in that: A plurality of groups of annular liquid nitrogen pipelines are arranged at the lower part of the bracket, and the liquid nitrogen pipelines are evenly distributed under the bracket; and the centers of the liquid nitrogen pipelines are connected to the lower part of the liquid nitrogen storage tank.

4. A sheep embryo freezing device according to claim 3, characterized in that: A shell is provided at the center above the bracket, the liquid nitrogen storage tank is arranged in the shell, the top of the liquid nitrogen storage tank and the shell are in an integrated structure, and a filling port for filling liquid nitrogen is provided at a position on the top of the shell corresponding to the liquid nitrogen storage tank, and a movably plugged sealing plug is provided at the filling port.

5. A sheep embryo freezing device according to claim 4, characterized in that: The shell is also provided with a battery pack for supplying power to the control panel, the electromagnetic valve and the temperature detection probe.

6. A sheep embryo freezing device according to claim 5, characterized in that: The shell is a double-layer structure, and evenly distributed electric heating wires are arranged in the gap in the middle of the double-layer shell. The electric heating wires are powered by a battery pack.

7. A sheep embryo freezing device according to claim 6, characterized in that: The box cover is an annular structure. When the box cover is buckled on the top of the box body, the shell body passes through the center of the box cover.

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