Liquid transfer mechanism and molecule direct diffusion detection device

Abstract

The utility model belongs to the field of molecule detection, and particularly relates to a liquid transfer mechanism and a molecule direct diffusion detection device.According to the liquid transfer mechanism, a driving piece is used for driving a puncture needle in a puncture assembly to puncture a puncture part on a storage tube, and the storage tube is communicated with a reaction tube through an infusion tube; when the liquid transfer mechanism is used for transferring the liquid between the storage tube and the reaction tube, operations such as cover opening, pipetting needle / dropper liquid suction, pipetting and cover closing are not needed, only the driving part needs to be pressed to move the liquid transfer mechanism, the operation is simple, and the risks of errors and biological exposure pollution are reduced. The molecular direct diffusion detection device with the liquid transfer mechanism is also simple to operate and convenient to use, and the risks of errors and biological exposure pollution are reduced.

Description

Technical Field

[0001] The present application relates to the field of molecular detection technology, and in particular to a liquid transfer mechanism and a molecular direct diffusion detection device. Background Art

[0002] The characteristic of molecular POCT (point-of-care testing) of "sample in, result out" not only directly lowers the threshold of nucleic acid testing, breaks the shackles of "four suites" of nucleic acid testing, but also benefits the miniaturization and portability of qPCR testing. The key to realizing molecular POCT is to simplify sample processing. Direct PCR is a technology that can achieve in vitro nucleic acid amplification without the need for additional nucleic acid extraction, eliminating the tedious procedure of extracting genomic DNA, and can be directly applied to rapid PCR testing.

[0003] The existing molecular detection experimental process using the direct amplification method is: after collecting a pharyngeal or nasal swab from the patient, put it into a cell preservation fluid tube for storage, then open the cover manually or by machine to absorb the sample liquid and add it to the PCR tube that has been filled with PCR reagents, and then perform amplification detection on the machine. Among them, when transferring the sample liquid to the PCR tube, at least the following process needs to be experienced: open the tube cover of the cell preservation fluid tube, absorb a certain amount of sample liquid through a pipette needle / dropper, add the absorbed sample liquid to the PCR tube, and screw on the tube cover of the cell preservation fluid tube. The above steps are numerous, and the operation is prone to errors, and there is also the risk of biological exposure contamination. Utility Model Content

[0004] The embodiments of the present application provide a liquid transfer mechanism and a molecular direct amplification detection device, which are intended to simplify the operational process of adding sample liquid to a reaction tube and reduce the risk of errors and biological exposure contamination.

[0005] To this end, according to one aspect of the present application, a liquid transfer mechanism is provided for transferring the liquid in a storage tube to a reaction tube, wherein the lower end of the storage tube has a puncture portion that can be pierced by a needle, and the liquid transfer mechanism comprises:

[0006] A carrier having a sample position for fixing the storage tube and a detection position for fixing the reaction tube;

[0007] The puncture assembly comprises a sliding member slidably disposed on the carrier and a puncture needle mounted on the sliding member, wherein the puncture portion on the storage tube is located on the movement path of the sliding member;

[0008] an infusion tube, one end of which is connected to the puncture needle and the other end of which is capable of communicating with the reaction tube; and

[0009] The driving member is slidably arranged on the carrier, and the movement path of the driving member intersects with the movement path of the sliding member. The driving member can push the sliding member during the movement on the carrier to make the puncture needle pierce the puncture part.

[0010] Optionally, the sliding member is provided with a push inclined surface, and during the movement of the driving member on the carrier, the driving member pushes the push inclined surface so that the sliding member drives the puncture needle to puncture the puncture portion; or

[0011] The driving member is provided with an abutting inclined surface, and during the movement of the driving member on the carrier, the abutting inclined surface pushes the sliding member, so that the sliding member drives the puncture needle to puncture the puncture part; or

[0012] The sliding member is provided with a push inclined surface, and the driving member is provided with a top inclined surface. During the movement of the driving member on the carrier, the top inclined surface pushes the push inclined surface, so that the sliding member drives the puncture needle to puncture the puncture part.

[0013] Optionally, the sliding member is slidably disposed on the carrier along a first direction, and the driving member is slidably disposed on the carrier along a second direction, and the second direction is perpendicular to the first direction.

[0014] Optionally, a guide portion extending along the first direction is provided on the carrier, the sliding member is slidably connected to the guide portion, one end of the guide portion is located on the movement path of the driving member, and the other end of the guide portion extends to the puncture portion.

[0015] Optionally, a guide portion extending along the second direction is provided on the carrier, and the driving member is slidably connected to the guide portion.

[0016] Optionally, in the vertical direction, the height of the detection position is lower than the height of the sample position, so that a height difference is formed between the storage tube and the reaction tube.

[0017] Optionally, the liquid transfer mechanism further includes a push rod, which is hinged to the driving member, and a receiving groove for receiving the push rod is provided on the carrier. Before the liquid transfer mechanism is used, the push rod can be flipped relative to the driving member and received in the receiving groove.

[0018] Optionally, the liquid transfer mechanism further comprises a piston cylinder, a piston disposed in the piston cylinder, and an air extraction pipe connected to the piston cylinder, and the other end of the air extraction pipe can be connected to the top of the reaction tube;

[0019] The piston is connected to the driving member, and a limiting portion is provided on the carrier. The movement path of the driving member has at least a starting position, an intermediate position and an end position. During the movement of the driving member from the starting position to the intermediate position, the piston cylinder is stationary relative to the driving member; when the driving member is in the intermediate position, the puncture needle pierces the puncture portion, and the limiting portion abuts against the piston cylinder; during the movement of the driving member from the intermediate position to the end position, the sliding member and the piston cylinder are both stationary relative to the carrier.

[0020] Optionally, the liquid transfer mechanism also includes an adapter, which is movably arranged on the driving member, and two needles are arranged on the adapter, and one end of the infusion tube away from the puncture needle and one end of the exhaust tube away from the piston cylinder are respectively connected to the two needles; on the movement path of the driving member, there is also a front position between the starting position and the middle position, and in the process of the driving member moving from the starting position to the front position, the driving member drives the adapter to drive the two needles to pierce the rubber plug of the reaction tube; in the process of the driving member moving from the front position to the middle position, the driving member pushes the sliding member to make the puncture needle pierce the puncture part; in the process of the driving member moving from the front position to the end position, the adapter is stationary relative to the carrier.

[0021] According to another aspect of the present application, a molecular direct diffusion detection device is provided, comprising:

[0022] A liquid transfer mechanism as described above;

[0023] A storage tube fixed to the sample position, wherein a storage liquid is stored in the storage tube, and a puncture portion that can be pierced by a needle is provided at the lower end of the storage tube; and

[0024] A reaction tube is fixed at the detection position, and detection reagent is stored in the reaction tube.

[0025] The liquid transfer mechanism and molecular direct expansion detection device provided by the present application have the following beneficial effects: compared with the prior art, the liquid transfer mechanism of the present application uses a driving member to drive the puncture needle in the puncture assembly to puncture the puncture portion on the storage tube, and connects the storage tube and the reaction tube through the infusion tube, so that the liquid in the storage tube can flow into the reaction tube through the infusion tube. The liquid transfer mechanism is used to transfer liquid between the storage tube and the reaction tube, and there is no need to open the cover, pipette needle / dropper aspirate liquid, pipette and close the cover. It only needs to press the driving member to move it, which is simple to operate and reduces the risk of errors and biological exposure contamination. The molecular direct expansion detection device with the liquid transfer mechanism is also simple to operate and easy to use, reducing the risk of errors and biological exposure contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0027] in:

[0028] Figure 1 is a schematic diagram of the external structure of a molecular direct diffusion detection device shown in one embodiment of the present application;

[0029] Figure 2 is a structural schematic diagram of a liquid transfer mechanism shown in an embodiment of the present application after the carrier is removed and the driving member is located in the starting position;

[0030] Figure 3 is a schematic cross-sectional structural diagram of a carrier of a liquid transfer mechanism shown in one embodiment of the present application;

[0031] Figure 4 It is a structural schematic diagram of a liquid transfer mechanism shown in an embodiment of the present application after the carrier is removed and the driving member is in the front position;

[0032] Figure 5 It is a structural schematic diagram of a liquid transfer mechanism shown in an embodiment of the present application after the carrier is removed and the driving member is located in the middle position;

[0033] Figure 6 is a schematic cross-sectional structural diagram of a driving member in a liquid transfer mechanism shown in an embodiment of the present application when the driving member is located at a stop position;

[0034] Figure 7 is a structural schematic diagram of a liquid transfer mechanism shown in an embodiment of the present application when the driving member and the adapter member are not separated;

[0035] Figure 8 It is a schematic diagram of the structure after the driving member and the adapter member in the liquid transfer mechanism are separated according to one embodiment of the present application.

[0036] Description of main component symbols:

[0037] 10. Storage tube; 11. Puncture portion; 12. Tube cap;

[0038] 20. Reaction tube; 21. Rubber stopper;

[0039] 100, carrier; 1001, first insertion port; 1002, second insertion port; 1003, storage slot; 101, guide portion; 102, guide portion; 103, first slide slot; 104, second slide slot; 110, separation structure;

[0040] 200, puncture assembly; 210, sliding member; 211, push ramp; 212, guide protrusion; 220, puncture needle;

[0041] 300, infusion tube;

[0042] 400, driving member; 401, abutting inclined surface; 410, guiding protrusion; 420, side plate; 421, slot;

[0043] 500, putter;

[0044] 600, piston cylinder; 610, first guide rib;

[0045] 700, piston;

[0046] 800, exhaust pipe;

[0047] 900, adapter; 910, side strip; 911, clamping protrusion; 920, connecting block; 930, third guide rib; 1000, needle. DETAILED DESCRIPTION

[0048] In order to facilitate the understanding of the present application, the present application will be described more comprehensively with reference to the relevant drawings below. The preferred embodiments of the present application are provided in the drawings. However, the present application can be implemented in many other different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present application more thorough and comprehensive.

[0049] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0050] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0051] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features.

[0052] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0053] It should also be noted that, in the embodiments of the present application, the same figure mark is used to represent the same component or the same part. For the same parts in the embodiments of the present application, the figure may only mark one of the parts or components as an example. It should be understood that the figure mark also applies to other identical parts or components.

[0054] As described in the background art, in existing molecular tests using direct amplification methods, when transferring sample liquid to a PCR tube, at least the following process is required: opening the cap of the cell preservation liquid tube, aspirating a certain amount of sample liquid through a pipette needle / dropper, adding the aspirated sample liquid to the PCR tube, and screwing on the cap of the cell preservation liquid tube. The above steps are numerous, prone to errors, and there is also the risk of biological exposure and contamination.

[0055] In order to solve the above problems, according to one aspect of the present application, an embodiment of the present application provides a liquid transfer mechanism for transferring the liquid in the storage tube to the reaction tube, and the lower end of the storage tube has a puncture portion that can be pierced by a needle, such as Figure 1-Figure 2 and Figure 4-Figure 5 As shown, the liquid transfer mechanism includes a carrier 100, a puncture assembly 200, an infusion tube 300 and a driving member 400. The carrier 100 has a sample position for fixing the storage tube 10 and a detection position for fixing the reaction tube 20. The puncture assembly 200 includes a sliding member 210 slidably arranged on the carrier 100 and a puncture needle 220 installed on the sliding member 210, and the puncture portion 11 on the storage tube 10 is located on the movement path of the sliding member 210. One end of the infusion tube 300 is connected to the puncture needle 220, and the other end can be connected to the reaction tube 20. The driving member 400 is slidably arranged on the carrier 100, and the movement path of the driving member 400 intersects with the movement path of the sliding member 210. The driving member 400 can push the sliding member 210 during the movement on the carrier 100, so that the puncture needle 220 punctures the puncture portion 11.

[0056] In the embodiment of the present application, the liquid transfer mechanism uses the driving member 400 to drive the puncture needle 220 in the puncture assembly 200 to puncture the puncture portion 11 on the storage tube 10, and connects the storage tube 10 and the reaction tube 20 through the infusion tube 300, so that the liquid in the storage tube 10 can flow into the reaction tube 20 through the infusion tube 300. The liquid transfer mechanism is used to transfer liquid between the storage tube 10 and the reaction tube 20, without opening the cover, pipetting needle / dropper aspirating liquid, pipetting and closing the cover, etc., and only needs to press the driving member 400 to move it, which is simple to operate and reduces the risk of errors and biological exposure contamination. The molecular direct amplification detection device with the liquid transfer mechanism is also simple to operate and easy to use, reducing the risk of errors and biological exposure contamination.

[0057] In one embodiment, the driving member 400 and the sliding member 210 can achieve push-pushing during the movement through a wedge-shaped push-pushing structure. Specifically, there are the following three ways:

[0058] In one implementation, the sliding member 210 is provided with a push inclined surface 211 , and when the driving member 400 moves on the carrier 100 , the sliding member 210 pushes the push inclined surface 211 so that the puncture needle 220 can puncture the puncture portion 11 .

[0059] In another implementation, the driving member 400 is provided with a resisting inclined surface 401 . When the driving member 400 moves on the carrier 100 , the resisting inclined surface 401 pushes the sliding member 210 , so that the sliding member 210 drives the puncture needle 220 to puncture the puncture portion 11 .

[0060] In yet another implementation, Figure 2 As shown, the sliding member 210 is provided with a push inclined surface 211, and the driving member 400 is provided with a top push inclined surface 401. During the movement of the driving member 400 on the carrier 100, the top push inclined surface 401 pushes the push inclined surface 211, so that the sliding member 210 drives the puncture needle 220 to puncture the puncture part 11. Preferably, in this embodiment, the push inclined surface 211 and the top push inclined surface 401 have the same inclination, so that the contact area is increased and the stability of the sliding member 210 during the movement is improved.

[0061] In one embodiment, if Figure 2 , Figure 4 and Figure 5 As shown, the sliding member 210 is slidably disposed on the carrier 100 along a first direction, and the driving member 400 is slidably disposed on the carrier 100 along a second direction, and the second direction is perpendicular to the first direction.

[0062] The sliding directions of the sliding member 210 and the driving member 400 are perpendicular to each other, so as to facilitate the determination of the movement tracks and strokes of the sliding member 210 and the driving member 400 respectively.

[0063] Exemplarily, the first direction is the horizontal direction in the figure, and the second direction is the vertical direction in the figure. At the same time, the second direction is also the height direction of the storage tube 10 and the reaction tube 20. This arrangement facilitates the application of force to the driving member 400, and when in use, the driving member 400 only needs to be pressed downward.

[0064] In a specific embodiment, in combination Figure 2 and Figure 3 As shown, a guide portion 101 extending along a first direction is provided on the carrier 100 , the sliding member 210 is slidably connected to the guide portion 101 , one end of the guide portion 101 is located on the movement path of the driving member 400 , and the other end of the guide portion 101 extends to the puncture portion 11 .

[0065] Understandably, combined Figure 3 As shown, when the liquid transfer mechanism is not in use, the sliding member 210 is located at one end of the guide portion 101 on the movement path of the driving member 400. At this time, the puncture needle 220 on the sliding member 210 does not pierce the puncture portion 11 at the lower end of the storage tube 10. When the driving member 400 is pressed to slide relative to the carrier 100, as shown in FIG. Figure 4-Figure 5 As shown, the driving member 400 can push the sliding member 210 , driving the sliding member 210 to move along the guiding portion 101 toward the puncture portion 11 and finally puncture the puncture portion 11 .

[0066] Specifically, the guide portion 101 on the carrier 100 serves to connect the sliding member 210 and guide the movement of the sliding member 210. The guide portion 101 can adopt a guide groove. Accordingly, the sliding member 210 (such as a slider) is provided with a guide protrusion 212 that slidably cooperates with the guide groove.

[0067] In a specific embodiment, in combination Figure 2 and Figure 3 As shown, a guide portion 102 extending along the second direction is disposed on the carrier 100 , and the driving member 400 is slidably connected to the guide portion 102 .

[0068] The guide portion 102 on the carrier 100 serves to connect the driving member 400 and guide the movement of the driving member 400. The guide portion 102 can be a guide groove. Accordingly, the driving member 400 (such as a driving frame or a driving block) is provided with a guide protrusion 410 that slidably cooperates with the guide groove.

[0069] In one embodiment, if Figure 2 As shown, in the vertical direction, the height of the detection position is lower than that of the sample position, so that a height difference is formed between the storage tube 10 and the reaction tube 20. With this design, after the storage tube 10 and the reaction tube 20 are connected through the infusion tube 300, the liquid in the storage tube 10 can enter the reaction tube 20. At the same time, the space above the detection position is used to install and arrange structures such as the driving member 400, so as to reasonably utilize the space.

[0070] In one embodiment, if Figure 1 and Figure 2 As shown, the liquid transfer mechanism also includes a push rod 500, which is hinged to the driving member 400. A storage groove 1003 for accommodating the push rod 500 is provided on the carrier 100. Before the liquid transfer mechanism is used, the push rod 500 can be flipped relative to the driving member 400 and stored in the storage groove 1003. When in use, the push rod 500 is flipped out of the storage groove 1003 and the push rod 500 is pressed.

[0071] The push rod 500 is provided to facilitate application of external force to the driving member 400 to drive its movement. At the same time, before the liquid transfer mechanism is used, the push rod 500 is flipped relative to the driving member 400 and stored in the storage groove 1003 to prevent the push rod 500 from being pressed by mistake.

[0072] For example, Figure 1 and Figure 6 As shown, the carrier 100 is a shell-like structure, and the sample position, detection position, puncture assembly 200, infusion tube 300 and driving member 400 are all arranged in the shell-like carrier 100, and the carrier 100 is used to protect the puncture assembly 200, infusion tube 300 and driving member 400. The top of the shell-like carrier 100 is provided with a first insertion port 1001 for inserting the storage tube 10 into the sample position, and the bottom of the shell-like carrier 100 is provided with a second insertion port 1002 for inserting the reaction tube 20 into the detection position. The storage groove 1003 for storing the push rod 500 is located at the top of the shell-like carrier 100.

[0073] In one embodiment, if Figure 2-Figure 6 As shown, the liquid transfer mechanism further includes a piston cylinder 600 , a piston 700 disposed in the piston cylinder 600 , and an exhaust pipe 800 connected to the piston cylinder 600 , and the other end of the exhaust pipe 800 can be connected to the top of the reaction tube 20 .

[0074] The piston 700 is connected to the driving member 400, and a limiting portion is provided on the carrier 100, which has at least a starting position ( Figure 2 The position shown in ), the middle position ( Figure 5 Position shown) and end position ( Figure 6In the process of the driving member 400 moving from the starting position to the intermediate position, the piston cylinder 600 is stationary relative to the driving member 400, that is, in this process, the piston cylinder 600 and the piston 700 do not move relative to each other; when the driving member 400 is in the intermediate position, the puncture needle 220 punctures the puncture portion 11, that is, the storage tube 10 and the reaction tube 20 are connected through the infusion tube 300, and the limiting portion abuts against the piston cylinder 600, limiting the piston cylinder 600 from continuing to move with the driving member 400; when the driving member 400 is in the intermediate position, the piston cylinder 600 is stationary relative to the driving member 400, that is, during this process, the piston cylinder 600 and the piston 700 do not move relative to each other; when the driving member 400 is in the intermediate position, the puncture needle 220 punctures the puncture portion 11, that is, the storage tube 10 and the reaction tube 20 are connected through the infusion tube 300, and the limiting portion abuts against the piston cylinder 600, limiting the piston cylinder 600 from continuing to move with the driving member 400; During the movement from the intermediate position to the end position, the sliding member 210 and the piston cylinder 600 are both stationary relative to the carrier 100. During this process, the piston 700 in the piston cylinder 600 moves relative to the piston cylinder 600 driven by the driving member 400, and the gas in the reaction tube 20 is pumped into the piston cylinder 600 through the exhaust pipe 800, forming a negative pressure in the reaction tube 20, and the liquid in the storage tube 10 is pumped into the reaction tube 20 through the infusion tube 300, thereby accelerating the quantitative transfer of the liquid.

[0075] Specifically, if Figure 5 and Figure 6 As shown, during the process of the driving member 400 moving from the middle position to the end position, the side surface of the driving member 400 slides and abuts against the side surface of the sliding member 210. That is, after the driving member 400 pushes the sliding member 210 to make the puncture needle 220 pierce the puncture portion 11 on the storage tube 10, the sliding member 210 is located on one side of the driving member 400, and the subsequent movement of the driving member 400 does not push the sliding member 210 and exert thrust on it, so that during the process of the driving member 400 moving from the middle position to the end position, the sliding member 210 is stationary relative to the carrier 100.

[0076] The carrier 100 is provided with a first slide groove 103 extending along the movement direction of the driving member 400. The length of the first slide groove 103 is the same as the movement distance of the driving member 400 from the starting position to the middle position. The outer wall of the piston cylinder 600 is provided with a first guide rib 610, and the first guide rib 610 is slidably arranged in the first slide groove 103. In the process of the driving member 400 moving from the starting position to the middle position, the first guide rib 610 slides in the first slide groove 103, and the two cooperate to guide the movement of the piston cylinder 600; in the middle position, the first guide rib 610 abuts against the lower end groove wall of the first slide groove 103, and the groove wall of the first slide groove 103 here forms a limiting portion, which limits the piston cylinder 600 from continuing to move downward with the driving member 400, so that the piston cylinder 600 is stationary relative to the carrier 100 in the process of the driving member 400 moving from the middle position to the end position.

[0077] In a specific embodiment, see Figure 2 and Figure 4-Figure 6The liquid transfer mechanism also includes an adapter 900, which is movably arranged on the driving member 400. Two needles 1000 are arranged on the adapter 900. The end of the infusion tube 300 away from the puncture needle 220 and the end of the suction tube 800 away from the piston cylinder 600 are respectively connected to the two needles 1000; on the movement path of the driving member 400, there is also a front position ( Figure 4 ), when the driving member 400 moves from the starting position to the front position, the driving member 400 drives the adapter 900 to drive the two needles 1000 to pierce the rubber plug 21 of the reaction tube 20, so that the end of the infusion tube 300 away from the puncture needle 220 and the end of the exhaust tube 800 away from the piston cylinder 600 are connected with the inside of the reaction tube 20; in the process of the driving member 400 moving from the initial position to the front position, the top inclined surface 401 gradually approaches and abuts against the push inclined surface 211, and in the process of the driving member 400 moving from the front position to the middle position, the top inclined surface 401 pushes the push inclined surface 211, so that the sliding member 210 drives the puncture portion 11 of the puncture needle 220, so that the storage tube 10 and the reaction tube 20 are connected through the infusion tube 300; in the process of the driving member 400 moving from the front position to the end position, the adapter 900 is stationary relative to the carrier 100.

[0078] Preferably, in order to improve the stability of the adapter 900 during movement, Figure 2 and Figure 3 As shown, a second slide groove 104 extending in the vertical direction is provided on the carrier 100 , and a third guide rib 930 slidably matched with the second slide groove 104 is provided on the adapter 900 .

[0079] Specifically, if Figure 7 and Figure 8As shown, the driving member 400 has two spaced-apart side panels 420, and the inner sides of the two side panels 420 are provided with card slots 421. The adapter 900 includes two spaced-apart side strips 910 and a connecting block 920 connected between one ends of the two side strips 910. Two needles 1000 are arranged on the connecting block 920, and the outer sides of the other ends of the two side strips 910 are provided with card protrusions 911. The adapter 900 is clamped in the card slots 421 on the inner sides of the two side panels 420 through the card protrusions 911 at the ends of the two side strips 910. A separation structure 110 is provided on the carrier 100 on the movement path of the two side plates 420. The separation structure 110 is two separation sheets. When the driving member 400 moves from the starting position to the front position, the driving member 400 drives the adapter 900 to move together to drive the two needles 1000 to pierce the rubber plug 21 of the reaction tube 20; in the process of the driving member 400 moving from the front position to the middle position, the two separation sheets are inserted into the relative inner sides of the two side plates 420. As the driving member 400 continues to move from the front position to the end position, the two separation sheets open the two side plates 420 to separate the protrusion 911 at the end of the side strip 910 from the slot 421 in the side plate 420, so that the adapter 900 is stationary relative to the carrier 100.

[0080] In summary, in one example, the storage tube 10 is a sampling tube, in which a cell preservation solution is stored, and the reaction tube 20 is a PCR tube, in which paraffin balls and PCR reagent freeze are stored. The entire use process of the liquid transfer mechanism is as follows:

[0081] 1. Install sampling tubes and PCR tubes at the sample position and detection position respectively;

[0082] 2. Open the tube cover 12 of the sampling tube, break the sampling test piece into the tube, and tighten the tube cover 12;

[0083] 3. Figure 1 As shown, flip up the push rod 500;

[0084] 4. Figure 2 and Figure 4 As shown, by pressing the push rod 500, the driving member 400 drives the two needles 1000 on the adapter 900 above the PCR tube to pierce the rubber plug 21 of the PCR tube;

[0085] 5. If Figure 5 As shown, the push rod 500 is continuously pressed, the adapter 900 above the PCR tube is separated from the driving member 400, and the driving member 400 drives the puncture needle 220 on the sliding member 210 on the side of the sample liquid tube to puncture the puncture portion 11 at the lower end of the sampling tube;

[0086] 6. If Figure 6As shown, continue to press the push rod 500. After the piston cylinder 600 reaches the limited position, the piston 700 moves downward under the drive of the driving member 400 to start extracting the gas in the PCR tube, and quantitatively extracts the liquid containing the sample in the sampling tube into the reconstituted reagent freeze-dried ball in the PCR tube.

[0087] According to another aspect of the present application, an embodiment of the present application further provides a molecular direct diffusion detection device, comprising the liquid transfer mechanism, the storage tube 10 and the reaction tube 20 in any of the above embodiments.

[0088] The storage tube 10 is fixed at the sample position, and the storage tube 10 stores a storage solution. The lower end of the storage tube 10 has a puncture portion 11 that can be pierced by a needle. The reaction tube 20 is fixed at the detection position, and the reaction tube 20 stores a detection reagent.

[0089] Specifically, a through hole is provided at the lower end of the storage tube 10 , and the through hole is sealed by a rubber cover, which forms the puncture portion 11 .

[0090] Since the molecular direct amplification detection device adopts the liquid transfer mechanism of the above-mentioned embodiment, during the process of transferring liquid between the storage tube 10 and the reaction tube 20, there is no need to open the cover, aspirate the liquid with a pipette needle / dropper, pipette and close the cover, and only needs to press the driving member 400 to move it. The operation is simple and reduces the risk of errors and biological exposure contamination.

[0091] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0092] The above embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the application. It should be pointed out that, for a person of ordinary skill in the art, several modifications and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the attached claims.

Claims

1. A liquid transfer mechanism, used for transferring liquid in a storage tube (10) to a reaction tube (20), wherein the lower end of the storage tube (10) has a puncture portion (11) that can be punctured by a needle, characterized in that: The liquid transfer mechanism comprises: A carrier (100) having a sample position for fixing the storage tube (10) and a detection position for fixing the reaction tube (20); The puncture assembly (200) comprises a sliding member (210) slidably disposed on the carrier (100) and a puncture needle (220) mounted on the sliding member (210), wherein the puncture portion (11) on the storage tube (10) is located on the movement path of the sliding member (210); an infusion tube (300), one end of which is connected to the puncture needle (220) and the other end of which is capable of communicating with the reaction tube (20); and A driving member (400) is slidably disposed on the carrier (100), and a movement path of the driving member (400) intersects with a movement path of the sliding member (210). The driving member (400) can push the sliding member (210) during movement on the carrier (100) so that the puncture needle (220) punctures the puncture portion (11).

2. The liquid transfer mechanism according to claim 1, characterized in that: The sliding member (210) is provided with a resisting inclined surface (211), and the driving member (400) pushes the resisting inclined surface (211) during the movement on the carrier (100), so that the sliding member (210) drives the puncture needle (220) to puncture the puncture portion (11); or The driving member (400) is provided with a resisting inclined surface (401), and when the driving member (400) moves on the carrier (100), the resisting inclined surface (401) pushes the sliding member (210), so that the sliding member (210) drives the puncture needle (220) to puncture the puncture portion (11); or The sliding member (210) is provided with a resisting inclined surface (211), and the driving member (400) is provided with a resisting inclined surface (401). When the driving member (400) moves on the carrier (100), the resisting inclined surface (211) is pushed by the resisting inclined surface (401), so that the sliding member (210) drives the puncture needle (220) to puncture the puncture part (11).

3. The liquid transfer mechanism according to claim 1, characterized in that: The sliding member (210) is slidably disposed on the carrier (100) along a first direction, and the driving member (400) is slidably disposed on the carrier (100) along a second direction, wherein the second direction is perpendicular to the first direction.

4. The liquid transfer mechanism according to claim 3, characterized in that: The carrier (100) is provided with a guide portion (101) extending along the first direction, the sliding member (210) is slidably connected to the guide portion (101), one end of the guide portion (101) is located on the movement path of the driving member (400), and the other end of the guide portion (101) extends to the puncture portion (11).

5. The liquid transfer mechanism according to claim 3, characterized in that: The carrier (100) is provided with a guide portion (102) extending along the second direction, and the driving member (400) is slidably connected to the guide portion (102).

6. The liquid transfer mechanism according to claim 1, characterized in that: In the vertical direction, the height of the detection position is lower than the height of the sample position, so that a height difference is formed between the storage tube (10) and the reaction tube (20).

7. The liquid transfer mechanism according to claim 1, characterized in that: The liquid transfer mechanism also includes a push rod (500), which is hinged to the driving member (400). The carrier (100) is provided with a receiving groove (1003) for receiving the push rod (500). Before the liquid transfer mechanism is used, the push rod (500) can be flipped relative to the driving member (400) and received in the receiving groove (1003).

8. The liquid transfer mechanism according to any one of claims 1 to 7, characterized in that: The liquid transfer mechanism further comprises a piston cylinder (600), a piston (700) disposed in the piston cylinder (600), and an exhaust pipe (800) connected to the piston cylinder (600), wherein the other end of the exhaust pipe (800) can be connected to the top of the reaction tube (20); The piston (700) is connected to the driving member (400), and a limiting portion is provided on the carrier (100). The movement path of the driving member (400) has at least a starting position, an intermediate position and a terminal position. During the movement of the driving member (400) from the starting position to the intermediate position, the piston cylinder (600) is stationary relative to the driving member (400); when the driving member (400) is in the intermediate position, the puncture needle (220) punctures the puncture portion (11), and the limiting portion abuts against the piston cylinder (600); during the movement of the driving member (400) from the intermediate position to the terminal position, the sliding member (210) and the piston cylinder (600) are both stationary relative to the carrier (100).

9. The liquid transfer mechanism according to claim 8, characterized in that: The liquid transfer mechanism further comprises a transfer member (900), wherein the transfer member (900) is movably arranged on the driving member (400), and two needles (1000) are arranged on the transfer member (900), and one end of the infusion tube (300) away from the puncture needle (220) and one end of the exhaust tube (800) away from the piston cylinder (600) are respectively connected to the two needles (1000); on the movement path of the driving member (400), there is also a front position between the starting position and the intermediate position, and the driving member (400) is moved from the starting position to the intermediate position. When the driving member (400) moves to the front position, the driving member (400) drives the adapter (900) to drive the two needles (1000) to pierce the rubber plug (21) of the reaction tube (20); when the driving member (400) moves from the front position to the middle position, the driving member (400) pushes the sliding member (210) to make the puncture needle (220) pierce the puncture portion (11); when the driving member (400) moves from the front position to the end position, the adapter (900) is stationary relative to the carrier (100).

10. A molecular direct diffusion detection device, characterized in that: include: The liquid transfer mechanism according to any one of claims 1 to 9; A storage tube (10) is fixed to the sample position, wherein a storage liquid is stored in the storage tube (10), and the lower end of the storage tube (10) has a puncture portion (11) that can be punctured by a needle; and A reaction tube (20) is fixed at the detection position, and a detection reagent is stored in the reaction tube (20).

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