Filtering device for stem cell separation and extraction

Abstract

The utility model discloses a filter device for stem cell separation and extraction, which belongs to the technical field of stem cell culture and comprises a bottom plate, a plurality of supports fixedly connected to the top of the bottom plate, a filter cartridge fixedly connected among the supports, a collecting mechanism arranged at the bottom of the filter cartridge, a flow guide component arranged in the filter cartridge, and a flushing mechanism arranged above a filter plate. The washing mechanism comprises a mounting block, and a plurality of fixing rods arranged in the circumferential direction are fixedly connected to the outer portion of the mounting block. The device has the beneficial effects that the flushing mechanism is arranged, the flushing mechanism comprises an annular pipe, a plurality of nozzles are fixedly connected to the outer wall of the annular pipe, the inner wall of the filter cartridge is flushed through a culture solution sprayed by the nozzles, stem cells on the inner wall of the filter cartridge are flushed away, and damage to the stem cells caused by direct utilization of a scraper blade is avoided; the power assembly is arranged, the annular pipe is driven by the power assembly to drive the spray head to move, and the washing integrity is improved; the filter plate is fixed at the bottom of the cylinder cover through the connecting rod, so that the filter plate can be conveniently taken out of the filter cylinder for cleaning.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture, in particular to a filtering device for separation and extraction of stem cells. Background Art

[0002] Stem cells are a type of cell with the potential for self-renewal and differentiation. Different separation methods are required for stem cell extraction depending on the cell type and tissue material. For example, neural stem cells are usually isolated by natural screening using serum-free culture medium, flow cytometry sorting, or immunomagnetic bead sorting. Filtration operations are often required when isolating and extracting stem cells.

[0003] After searching, the Chinese patent announcement number is CN220520493U, which discloses a filtering device for separation and extraction of stem cells. In the filtering device for separation and extraction of stem cells, the material enters the filter box through the feed port, and is stirred by rotating the scraper. While stirring, the scraper can scrape the inner wall of the filter box and the upper end of the filter plate to avoid the material sticking to the inner wall of the filter box and the upper end of the filter plate, reducing residue and clogging. The whole structure is detachable, which is convenient for thorough cleaning after later disassembly. The box port plug at the upper end of the collection box forms a collection structure with a sealing structure. The feed structure on the box port plug adopts a plug-in structure. The box port plug is automatically sealed after the guide needle is drawn out to avoid material contamination in the collection box. The support frame and the first motor are connected by bolts, the first motor and the first rotating shaft are connected by a coupling, and the first rotating shaft is connected to the bearing seat outside the filter box to form an adjustable support structure. The filter box can be driven to rotate by the first motor, so that the filter box can be tilted to a certain angle, thereby facilitating cleaning of the inside of the filter box, saving time and effort when cleaning.

[0004] However, a certain number of stem cells may adhere to the inner wall of the filter box, and directly scraping them with a scraper may damage the stem cells. Utility Model Content

[0005] The purpose of the utility model is to provide a filtering device for separation and extraction of stem cells in order to solve the above problems.

[0006] The utility model achieves the above-mentioned purpose through the following technical solutions:

[0007] A filtering device for separating and extracting stem cells comprises a bottom plate, a plurality of brackets are fixedly connected to the top of the bottom plate, a filter cartridge is fixedly connected between the brackets, a cartridge cover is detachably connected to the top of the filter cartridge, a connecting rod is fixedly connected to the bottom of the cartridge cover, a filter plate is fixedly connected to the bottom of the connecting rod, a collecting mechanism is arranged at the bottom of the filter cartridge, a flow guide assembly is arranged in the filter cartridge, a flushing mechanism is arranged above the filter plate, the flushing mechanism comprises a mounting block, a plurality of circumferentially arranged fixing rods are fixedly connected to the outside of the mounting block, an annular tube is fixedly connected to the other end of the fixing rod, a plurality of nozzles are fixedly connected to the outer wall of the annular tube, the nozzles face the inner wall of the filter cartridge, the flushing mechanism further comprises a power assembly and a liquid supply assembly, the power assembly is used to drive the mounting block to move up and down, and the liquid supply assembly is used to provide the annular tube with liquid required for flushing.

[0008] Preferably, the power assembly includes a rotating motor fixedly connected to the top of the cylinder cover, the output end of the rotating motor is connected to a screw through a coupling, the filter plate is rotatably connected to the bottom end of the screw, the mounting block is threadedly connected to the screw, and the mounting block is slidably connected to the connecting rod.

[0009] Preferably, the liquid supply assembly includes a storage box fixedly connected to the top of the base plate, a pump fixedly connected to the top of the storage box, a water pipe connected to the liquid outlet of the pump, a connecting pipe threadedly connected to the other end of the water pipe, a fixed pipe also threadedly connected inside the connecting pipe, the other end of the fixed pipe fixedly connected to the top of the cylinder cover, a bellows connected between the cylinder cover and the annular tube, and a cavity connecting the bellows and the fixed pipe is provided in the cylinder cover.

[0010] Preferably, the collecting mechanism comprises a collecting box fixedly connected to the top of the bottom plate, a through hole is provided on the top of the collecting box, and a box opening plug is provided in the through hole.

[0011] Preferably, the guide assembly includes a funnel slidably connected between the inner walls of the filter cartridge, the bottom of the funnel is fixedly connected to a guide tube, the bottom of the guide tube extends into the box port plug, the guide tube is slidably connected to the bottom wall of the filter cartridge, the outer wall of the guide tube is fixedly connected to a push handle, and the push handle is located outside the filter cartridge.

[0012] Preferably, the box port plug is slidably connected to the flow guide pipe.

[0013] The beneficial effects are as follows: a flushing mechanism is provided, the flushing mechanism includes an annular tube, the outer wall of the annular tube is fixedly connected to a plurality of nozzles, the culture fluid sprayed by the nozzles is used to flush the inner wall of the filter cartridge, the stem cells on the inner wall of the filter cartridge are flushed away, and direct use of the scraper to damage the stem cells is avoided; a power assembly is provided, the annular tube is driven by the power assembly to drive the nozzle to move, and the integrity of the flushing is improved; the filter plate is fixed to the bottom of the cartridge cover by a connecting rod, and the filter plate is conveniently taken out of the filter cartridge for cleaning.

[0014] The additional technical features and advantages of the present invention will be more clearly explained in the following description, or can be understood through the specific practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the present invention, but do not constitute a limitation to the present invention. In the accompanying drawings:

[0016] Figure 1 This is a schematic diagram of the overall structure of a filtration device for stem cell separation and extraction according to the utility model;

[0017] Figure 2 This is a front view of a filtration device for separation and extraction of stem cells according to the utility model;

[0018] Figure 3 This is a front view of the internal structure of a filtration device for stem cell separation and extraction according to the utility model;

[0019] Figure 4 This is a top view of a filtration device for stem cell separation and extraction according to the utility model;

[0020] Figure 5 It is a schematic diagram of an annular tube of a filtering device for stem cell separation and extraction described in the utility model.

[0021] The description of the accompanying drawings is as follows: 1. bottom plate; 2. bracket; 3. filter cartridge; 301. cartridge cover; 302. funnel; 303. guide tube; 304. push handle; 305. connecting rod; 306. filter plate; 4. collecting box; 401. box mouth plug; 501. mounting block; 502. fixing rod; 503. annular tube; 504. nozzle; 505. rotating motor; 506. screw; 601. storage box; 602. pump; 603. water pipe; 604. fixing pipe; 605. connecting pipe; 606. bellows. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0023] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0024] The utility model is further described below in conjunction with the accompanying drawings:

[0025] like Figure 1-Figure 5 As shown, a filtering device for separation and extraction of stem cells includes a bottom plate 1, a plurality of brackets 2 are fixedly connected to the top of the bottom plate 1, filter cartridges 3 are fixedly connected between the brackets 2, a cartridge cover 301 is connected to the top of the filter cartridge 3 by bolts, a feeding port is provided on the top of the cartridge cover 301, a sealing plug is provided in the feeding port, a connecting rod 305 is fixedly connected to the bottom of the cartridge cover 301, a filter plate 306 is fixedly connected to the bottom of the connecting rod 305, an edge of the filter plate 306 is in close contact with the inner wall of the filter cartridge 3, but does not affect the up and down sliding of the filter plate 306.

[0026] A collecting mechanism is provided at the bottom of the filter cartridge 3, and the collecting mechanism includes a collecting box 4 bolted to the top of the base plate 1, a through hole is provided on the top of the collecting box 4, a box port plug 401 is provided in the through hole, and the box port plug 401 is a prior art, and when the items in the box port plug 401 are pulled out, the box port plug 401 can be automatically sealed.

[0027] A flow guide component is provided in the filter cartridge 3, and the flow guide component includes a funnel 302 slidably connected between the inner walls of the filter cartridge 3, a flow guide tube 303 is fixedly connected to the bottom of the funnel 302, a control valve is provided in the flow guide tube 303, the bottom of the flow guide tube 303 extends into the box port plug 401, the flow guide tube 303 is slidably connected to the bottom wall of the filter cartridge 3, a push handle 304 is fixedly connected to the outer wall of the flow guide tube 303, and the push handle 304 is located outside the filter cartridge 3. When the collection box 4 needs to be replaced, the flow guide tube 303 and the funnel 302 are pushed upward by the push handle 304 to pull the flow guide tube 303 out of the box port plug 401, and the box port plug 401 and the flow guide tube 303 are slidably connected.

[0028] A flushing mechanism is arranged above the filter plate 306, and the flushing mechanism includes a mounting block 501, and the outside of the mounting block 501 is fixedly connected with a plurality of circumferentially arranged fixing rods 502, and the other end of the fixing rod 502 is fixedly connected with an annular tube 503, and the outer wall of the annular tube 503 is fixedly connected with a plurality of nozzles 504, and the nozzles 504 face the inner wall of the filter cylinder 3. The flushing mechanism also includes a power component and a liquid supply component, and the power component is used to drive the mounting block 501 to move up and down, and the liquid supply component is used to provide the annular tube 503 with the liquid required for flushing.

[0029] The power assembly includes a rotating motor 505 bolted to the top of the cylinder cover 301, the output end of the rotating motor 505 is connected to a screw rod 506 through a coupling, the filter plate 306 is rotatably connected to the bottom end of the screw rod 506, the mounting block 501 is threadedly connected to the screw rod 506, and the mounting block 501 is slidably connected to the connecting rod 305.

[0030] The liquid supply assembly includes a storage box 601 bolted to the top of the base plate 1, a pump 602 is bolted to the top of the storage box 601, a liquid outlet of the pump 602 is connected to a water pipe 603, the other end of the water pipe 603 is threadedly connected to a connecting pipe 605, and the other end of the connecting pipe 605 is also threadedly connected to a fixed pipe 604. By screwing the connecting pipe 605, the connecting pipe 605 and the fixed pipe 604 or the water pipe 603 can be separated, so as to facilitate the disassembly of the cylinder cover 301. The other end of the fixed pipe 604 is fixedly connected to the top of the cylinder cover 301, and a bellows 606 is connected between the cylinder cover 301 and the annular tube 503. A cavity is opened in the cylinder cover 301 to connect the bellows 606 with the fixed pipe 604.

[0031] Working principle: When in use, the culture fluid containing stem cells is poured into the filter cartridge 3, and the stem cells are screened through the filter plate 306. The excess culture fluid enters the collection box 4 through the funnel 302 and the guide tube 303 for collection. After the screening is completed, the pump 602 is started to transport the culture fluid without stem cells in the storage box 601 to the fixed pipe 604 through the water pipe 603, and then transported from the fixed pipe 604 to the corrugated pipe 606, and finally enters the annular pipe 503, and is sprayed toward the inner wall of the filter cartridge 3 through the nozzle 504, so as to flush the inner wall of the filter cartridge 3, and start The rotating motor 505 drives the screw rod 506 to rotate, and the screw rod 506 drives the mounting block 501 to drive the fixing rod 502 and the annular tube 503 to move downward, so as to wash away the stem cells adhered to the inner wall of the filter cartridge 3, and collect the stem cells on the filter plate 306. When the filter plate 306 needs to be cleaned, the connecting tube 605 is rotated to separate the fixing tube 604 from the connecting tube 605 and the water pipe 603, and then the bolts between the filter cartridge 3 and the cartridge cover 301 are removed, and the cartridge cover 301, the filter plate 306, the annular tube 503, etc. are all taken out for easy cleaning.

[0032] The above shows and describes the basic principles, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A filtration device for stem cell separation and extraction, comprising a bottom plate (1), a plurality of brackets (2) are fixedly connected to the top of the bottom plate (1), a filter cartridge (3) is fixedly connected between the brackets (2), a cartridge cover (301) is detachably connected to the top of the filter cartridge (3), a connecting rod (305) is fixedly connected to the bottom of the cartridge cover (301), a filter plate (306) is fixedly connected to the bottom of the connecting rod (305), a collecting mechanism is arranged at the bottom of the filter cartridge (3), and a flow guide component is arranged inside the filter cartridge (3), characterized in that: A flushing mechanism is arranged above the filter plate (306), the flushing mechanism comprising a mounting block (501), the outside of the mounting block (501) being fixedly connected to a plurality of circumferentially arranged fixing rods (502), the other end of the fixing rod (502) being fixedly connected to an annular tube (503), the outer wall of the annular tube (503) being fixedly connected to a plurality of nozzles (504), the nozzles (504) facing the inner wall of the filter cylinder (3), the flushing mechanism further comprising a power assembly and a liquid supply assembly, the power assembly being used to drive the mounting block (501) to move up and down, the liquid supply assembly being used to provide the annular tube (503) with liquid required for flushing.

2. A filtration device for separation and extraction of stem cells according to claim 1, characterized in that: The power assembly comprises a rotating motor (505) fixedly connected to the top of the cylinder cover (301); the output end of the rotating motor (505) is connected to a screw rod (506) via a coupling; the filter plate (306) is rotatably connected to the bottom end of the screw rod (506); the mounting block (501) is threadedly connected to the screw rod (506); and the mounting block (501) is slidably connected to the connecting rod (305).

3. A filtration device for separation and extraction of stem cells according to claim 1, characterized in that: The liquid supply assembly comprises a storage box (601) fixedly connected to the top of the base plate (1); a pump (602) is fixedly connected to the top of the storage box (601); a liquid outlet of the pump (602) is connected to a water pipe (603); a connecting pipe (605) is threadedly connected to the other end of the water pipe (603); a fixed pipe (604) is also threadedly connected inside the connecting pipe (605); the other end of the fixed pipe (604) is fixedly connected to the top of the cylinder cover (301); a bellows (606) is connected between the cylinder cover (301) and the annular pipe (503); a cavity is provided inside the cylinder cover (301) for communicating the bellows (606) with the fixed pipe (604).

4. A filtration device for separation and extraction of stem cells according to claim 1, characterized in that: The collecting mechanism comprises a collecting box (4) fixedly connected to the top of the bottom plate (1); a through hole is provided on the top of the collecting box (4), and a box opening plug (401) is provided in the through hole.

5. A filtration device for separation and extraction of stem cells according to claim 4, characterized in that: The flow guide assembly comprises a funnel (302) slidably connected between the inner walls of the filter cartridge (3); a flow guide tube (303) is fixedly connected to the bottom of the funnel (302); the bottom of the flow guide tube (303) extends into the box port plug (401); the flow guide tube (303) is slidably connected to the bottom wall of the filter cartridge (3); a push handle (304) is fixedly connected to the outer wall of the flow guide tube (3); and the push handle (304) is located outside the filter cartridge (3).

6. A filtration device for separation and extraction of stem cells according to claim 5, characterized in that: The box port plug (401) is slidably connected to the flow guide pipe (303).

Images