Organ-like chip device convenient for drug testing

Abstract

The utility model discloses an organoid chip device convenient for drug testing, which relates to the technical field of biomedical engineering and comprises a shell, a mounting bracket, a quick assembly and a culture assembly, the mounting bracket is fixedly arranged at the top of the shell, and an external hose is arranged on the inner surface of the mounting bracket; and the quick mounting assembly is fixedly arranged on the inner surface of the shell and is used for quickly mounting and butting the mounting bracket and the external hose. The knob is manually screwed to drive the threaded sleeve to rotate, and the threaded sleeve extends to the inner surface of the fixed seat through the top of the mounting bracket. The outer surface of the threaded sleeve is in threaded connection with the inner surface of the fixing base and moves downwards during rotation. The inserting rod is inserted into the inner surface of the locking support, the threaded sleeve moves downwards to extrude the top slope of the locking support, and the locking support gets close to lock the inserting rod. The device can quickly fasten the knob and the mounting bracket, facilitates the installation of an external hose and the introduction of medicines, is simple and convenient to operate, and provides convenience for operators.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical engineering, in particular to an organoid chip device which is convenient for drug testing. Background Art

[0002] Organoid chip technology is a revolutionary innovation in the field of biomedical engineering. It stems from the recognition of the limitations of traditional drug testing methods. For a long time, drug development has relied on animal models and in vitro cell culture systems, but these methods have many shortcomings, such as the great physiological differences between animal models and humans, and the inability of in vitro culture systems to fully simulate the in vivo environment.

[0003] In the prior art, the organoid chip device is an innovative technology in the field of biomedical engineering, which simulates the microscopic physiological environment and function of human organs. This device is usually made of microfluidic chip technology, which can create tiny channels and chambers on the chip. These channels and chambers can be designed to simulate specific human tissues or organs, such as lungs, heart, liver, kidneys, etc. The organoid chip device for high-throughput drug testing recorded in the patent document with announcement number CN217757476U provides a stable flow microenvironment for the cultivation of organoids by setting up upper and lower chips, and can also automatically form a drug concentration gradient. Drug testing is carried out directly after cultivation, which provides important reference value for realizing automated high-throughput analysis of organoids and provides commercial application potential for organoid drug testing.

[0004] However, when the device is in use, it is not convenient to quickly tighten and install the drug pipeline. The inconvenience of quick installation means that when building or replacing the organoid chip system, more time is required to complete the connection and fixation of the pipeline, which will reduce the efficiency of the experiment. Failure to tighten may lead to insufficient sealing, causing liquid leakage, affecting the stability of the microenvironment within the chip, and further affecting the survival and function of cells or tissues.

[0005] Based on this, organ-on-a-chip devices that facilitate drug testing are now available, which can eliminate the drawbacks of existing devices. Summary of the invention

[0006] The purpose of the utility model is to provide an organoid chip device that is convenient for drug testing, so as to solve the problems in the background technology.

[0007] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0008] An organoid chip device for facilitating drug testing comprises: a housing;

[0009] A mounting bracket, the mounting bracket is fixedly arranged on the top of the shell, and an external hose is arranged on the inner surface of the mounting bracket;

[0010] A quick-install component, which is fixedly arranged on the inner surface of the shell and is used for quickly installing and docking the mounting bracket and the external hose;

[0011] The culture component is fixedly arranged on the inner surface of the shell and is used for mixed culture of drugs and organoids.

[0012] On the basis of the above technical solutions, the present invention also provides the following optional technical solutions:

[0013] In an optional solution: the quick-release assembly includes a fixing seat, which is fixedly arranged on the inner surface of the shell, a knob is arranged above the fixing seat, a threaded sleeve is fixedly connected to the bottom of the knob, and the outer surface of the threaded sleeve is threadedly connected to the inner surface of the fixing seat.

[0014] In an optional solution, a locking assembly is fixedly provided at the bottom of the knob for locking the knob.

[0015] In an optional solution: the locking assembly includes an insertion rod, which is fixedly arranged at the bottom of the knob, the inner surface of the fixing seat is fixedly connected to the locking bracket, and the outer surface of the insertion rod is slidably connected to the inner surface of the locking bracket.

[0016] In an optional scheme: the culture component includes a first microfluidic inlet, the first microfluidic inlet is fixedly arranged on the inner surface of the shell, the inner surface of the shell is provided with a microchannel, the bottom end of the first microfluidic inlet is connected to the microchannel, the end of the microchannel is connected to the epidermal cell transition zone, the top of the epidermal cell transition zone is connected to the organoid culture chamber, the end of the microchannel is connected to the first microfluidic outlet, the top of the epidermal cell transition zone is connected to the second microfluidic inlet, and the top of the epidermal cell transition zone is connected to the second microfluidic outlet.

[0017] In an optional solution: a thread groove matching the threaded sleeve is formed on the inner surface of the mounting bracket.

[0018] In an optional solution, an extrusion slope matching the threaded sleeve is provided on the top of the locking bracket.

[0019] In an optional solution, the top end of the first microfluid inlet is matched with the bottom end of the external hose.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] 1. The utility model manually turns the knob to drive the threaded sleeve to rotate, and the threaded sleeve extends through the top of the mounting bracket to the inner surface of the fixing seat. The outer surface of the threaded sleeve is threadedly connected to the inner surface of the fixing seat and moves downward when rotating. The plug rod is inserted into the inner surface of the locking bracket, and the threaded sleeve moves downward to squeeze the top slope of the locking bracket, so that the locking bracket is close to the locking plug rod. The device can quickly tighten the knob and the mounting bracket, facilitate the installation of the external hose, facilitate the introduction of drugs, and is easy to operate, providing convenience for operators.

[0022] 2. The utility model achieves drug introduction by connecting the external hose to the microfluidic outlet. The drug enters the epithelial cell transition zone through the microchannel and is tested in the organoid culture compartment. The setting of the microfluidic inlet and outlet facilitates drug circulation and testing and improves experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a structural schematic diagram of the utility model.

[0024] Figure 2 It is a structural schematic diagram of the quick-install component in the utility model.

[0025] Figure 3 It is a structural schematic diagram of the threaded sleeve in the utility model.

[0026] Figure 4 It is a schematic cross-sectional structural diagram of the fixing seat in the utility model.

[0027] Figure 5 It is a schematic cross-sectional structural diagram of the shell in the utility model.

[0028] Notes on figure numbers: 1. Shell; 2. Mounting bracket; 3. External hose; 4. Fixing seat; 5. Knob; 6. Threaded sleeve; 7. Insert rod; 8. Locking bracket; 9. First microfluidic inlet; 10. Microchannel; 11. Skin cell transition zone; 12. Organoid culture compartment; 13. First microfluidic outlet; 14. Second microfluidic inlet; 15. Second microfluidic outlet. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments.

[0030] In one embodiment, Figure 1-Figure 5As shown, an organoid chip device for drug testing includes: a shell 1, a mounting bracket 2, a quick assembly and a culture assembly; the mounting bracket 2 is fixedly arranged on the top of the shell 1, and an external hose 3 is arranged on the inner surface of the mounting bracket 2; the quick assembly is fixedly arranged on the inner surface of the shell 1, and is used for quickly mounting and docking the mounting bracket 2 and the external hose 3; the culture assembly is fixedly arranged on the inner surface of the shell 1, and is used for mixed culture of drugs and organoids, the arrangement of the mounting bracket 2 facilitates the quick docking and installation of the external hose 3 and the first microfluid inlet 9, and the arrangement of the external hose 3 facilitates the intake of drugs from the outside;

[0031] In one embodiment, Figure 2 and Figure 3 As shown, the quick-release assembly includes a fixing seat 4, which is fixedly arranged on the inner surface of the shell 1, and a knob 5 is arranged above the fixing seat 4. A threaded sleeve 6 is fixedly connected to the bottom of the knob 5. The outer surface of the threaded sleeve 6 is threadedly connected to the inner surface of the fixing seat 4. A locking assembly is fixedly arranged at the bottom of the knob 5 for locking the knob 5. A threaded groove matched with the threaded sleeve 6 is provided on the inner surface of the mounting bracket 2, which is convenient for the threaded sleeve 6 to move downward when rotating. The setting of the fixing seat 4 is convenient for the threaded sleeve 6 to move downward when rotating. The setting of the knob 5 is convenient for the operator to manually turn the knob 5 to drive the threaded sleeve 6 to rotate.

[0032] In one embodiment, Figure 3 and Figure 4 As shown, the locking assembly includes an insert rod 7, which is fixedly arranged at the bottom of the knob 5. The inner surface of the fixing seat 4 is fixedly connected with a locking bracket 8. The outer surface of the insert rod 7 is slidably connected to the inner surface of the locking bracket 8. The top of the locking bracket 8 is provided with an extrusion slope that is compatible with the threaded sleeve 6, so that when the threaded sleeve 6 moves downward, the extrusion slope is squeezed, so that the locking bracket 8 moves closer to the center, thereby locking the insert rod 7.

[0033] In one embodiment, Figure 5As shown, the culture component includes a first microfluidic inlet 9, which is fixedly arranged on the inner surface of the shell 1, and a microchannel 10 is arranged on the inner surface of the shell 1. The bottom end of the first microfluidic inlet 9 is connected to the microchannel 10, and the end of the microchannel 10 is connected to the epidermal cell transition zone 11. The top of the epidermal cell transition zone 11 is connected to an organoid culture compartment 12, and the end of the microchannel 10 is connected to a first microfluidic outlet 13, and the top of the epidermal cell transition zone 11 is connected to a second microfluidic inlet 14, and the top of the epidermal cell transition zone 11 is connected to a second microfluidic outlet 15. The top of the first microfluidic inlet 9 is adapted to the bottom end of the external hose 3, so as to facilitate the docking of the external hose 3 with the first microfluidic inlet 9. The setting of the microchannel 10 facilitates the circulation of drugs, the setting of the epidermal cell transition zone 11 facilitates the cultivation of cell bodies, and the setting of the organoid culture compartment 12 enables cells to simulate their growth and development conditions in vivo in vitro.

[0034] The above embodiment discloses an organoid chip device that is convenient for drug testing, wherein the knob 5 is manually turned to drive the threaded sleeve 6 to rotate, the threaded sleeve 6 passes through the top of the mounting bracket 2 and extends to the inner surface of the fixing seat 4. Since the outer surface of the threaded sleeve 6 is threadedly connected with the inner surface of the fixing seat 4, the threaded sleeve 6 moves downward when rotating, and the insertion rod 7 is inserted into the inner surface of the locking bracket 8. When the threaded sleeve 6 moves downward, it squeezes the squeezing slope on the top of the locking bracket 8, so that the locking bracket 8 is squeezed and moved closer to the middle, and the insertion rod 7 is locked, so that the rotation is reversed. The button 5 and the mounting bracket 2 are quickly fastened, and the device can be quickly installed on the external hose 3, so as to facilitate the introduction of drugs, which brings convenience to the operator. The external hose 3 is connected to the first microfluid outlet 13 to introduce drugs. The drugs are introduced into the epithelial cell transition zone 11 through the microchannel 10, and the drugs are tested through the organoid culture compartment 12. The first microfluid inlet 9, the first microfluid outlet 13, the second microfluid inlet 14 and the second microfluid outlet 15 are arranged to facilitate drug circulation testing, thereby improving the experimental efficiency.

[0035] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art who is familiar with the present technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. Organoid chip devices that facilitate drug testing, including: Housing (1); A mounting bracket (2), the mounting bracket (2) being fixedly arranged on the top of the housing (1), and an external hose (3) being arranged on the inner surface of the mounting bracket (2); The invention is characterized in that it also includes a quick-install component, which is fixedly arranged on the inner surface of the shell (1) and is used for quickly installing and docking the mounting bracket (2) and the external hose (3), and the quick-install component includes a fixing seat (4), which is fixedly arranged on the inner surface of the shell (1), a knob (5) is arranged above the fixing seat (4), and a threaded sleeve (6) is fixedly connected to the bottom of the knob (5), and the outer surface of the threaded sleeve (6) is threadedly connected to the inner surface of the fixing seat (4); A culture component, which is fixedly arranged on the inner surface of the shell (1) and is used for mixed culture of drugs and organoids; A locking assembly is fixedly arranged at the bottom of the knob (5) for locking the knob (5), wherein the locking assembly comprises an insertion rod (7) which is fixedly arranged at the bottom of the knob (5), a locking bracket (8) is fixedly connected to the inner surface of the fixing seat (4), and an outer surface of the insertion rod (7) is slidably connected to the inner surface of the locking bracket (8).

2. The organoid chip device for facilitating drug testing according to claim 1, characterized in that: The culture component comprises a first microfluid inlet (9), the first microfluid inlet (9) is fixedly arranged on the inner surface of the shell (1), the inner surface of the shell (1) is provided with a microchannel (10), the bottom end of the first microfluid inlet (9) is connected to the microchannel (10), the end of the microchannel (10) is connected to the epidermal cell transition zone (11), the top of the epidermal cell transition zone (11) is connected to the organoid culture chamber (12), the end of the microchannel (10) is connected to the first microfluid outlet (13), the top of the epidermal cell transition zone (11) is connected to the second microfluid inlet (14), and the top of the epidermal cell transition zone (11) is connected to the second microfluid outlet (15).

3. The organoid chip device for facilitating drug testing according to claim 1, characterized in that: The inner surface of the mounting bracket (2) is provided with a thread groove matched with the threaded sleeve (6).

4. The organoid chip device for facilitating drug testing according to claim 1, characterized in that: The top of the locking bracket (8) is provided with an extrusion slope that matches the threaded sleeve (6).

5. The organoid chip device for facilitating drug testing according to claim 2, characterized in that: The top end of the first microfluid inlet (9) is adapted to the bottom end of the external hose (3).

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