Microplate reader detection plate
By designing the microplate reader detection plate, using the combination of locking parts and sealing rings, the problems of large amount of sample loading of the enzyme plate and sprinkling of samples are solved, and the stable installation of the microplate and the accuracy of the detection results are achieved, which is suitable for more experiments.
Patent Information
- Application Number
- CN202421534925.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-01
- Publication Date
- 2025-05-06
- Estimated Expiration
- 2034-07-01
AI Technical Summary
The amount of samples added by the existing enzyme label plate is large. Using a 96-well enzyme label plate, 100 to 200 ul of samples are required. The enzyme label plate is directly placed in the tray. When placed or taken out, the sample to be tested is easily spilled out, dirtying the tray and affecting the detection results.
A microplate reader detection plate is designed, including a bottom plate and a cover plate. The bottom plate is equipped with a receiving groove and a first hole groove, and the cover plate is equipped with a second hole groove and a groove. The locking member is used to achieve the tightening of the microplate. A sealing ring is provided on the microplate. The pressure plate of the cover plate is opposite to the sealing ring to prevent sample splashing.
It realizes the stable installation of microplate, avoids sample twitching, simplifies operation, improves detection accuracy, and combines the cuvette module with microplate, which is suitable for more experiments and reduces the problems caused by large usage and inconsistent optical pathways.
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Figure CN222838075U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of biomedical detection, in particular to an enzyme marker detection plate. Background Art
[0002] At present, ELISA instruments can usually only use ELISA plates, which are suitable for high-throughput sample detection. 96-well plates are usually used, and 96 samples can be detected on one plate. However, the sample loading volume of ELISA plates is large. When using 96-well ELISA plates, 100 to 200 ul of sample needs to be loaded, and the ELISA plates are usually placed directly in the tray. When putting in or taking out, it is easy to cause the samples to be tested in the ELISA plates to spill, dirty the tray and affect the test results. Utility Model Content
[0003] In view of the deficiencies in the prior art, the utility model provides an ELISA test plate to solve the technical problems in the related art that the sample loading volume of the existing ELISA plate is large, 100 to 200 ul of sample needs to be loaded when using a 96-well ELISA plate, and the ELISA plate is usually placed directly in a tray, which easily causes the test sample in the ELISA plate to spill when being placed in or taken out, soiling the tray and affecting the test results.
[0004] The utility model provides an enzyme labeling instrument detection plate, comprising:
[0005] The bottom plate has a receiving groove for placing the micro-well plate, wherein a plurality of first hole grooves are arranged in a rectangular array in the receiving groove, and the bottom of each first hole groove is transparent;
[0006] A cover plate is rotatably connected to the bottom plate, wherein the cover plate is provided with a plurality of second holes in a rectangular array, and the bottom of each second hole is transparent and corresponds to the first hole one by one; and
[0007] The locking piece is used to lock the bottom plate and the cover plate toward one side so as to fasten the micro-well plate in the receiving groove.
[0008] Furthermore, a sealing ring is provided on the microplate for storing samples; a groove is provided on the side of the cover plate facing the bottom plate, a pressing plate is provided in the groove to abut against the sealing ring, and a through hole is provided at the bottom of the pressing plate which penetrates vertically and faces the second hole groove.
[0009] Furthermore, the bottom plate also has a accommodating cavity, the cover plate covers the accommodating cavity, and the accommodating groove is arranged in the accommodating cavity.
[0010] Furthermore, the accommodating cavity and the cover plate are both provided with limiting holes, and the limiting holes are used to install limiting members to limit the microporous plate and the pressing plate.
[0011] Furthermore, the locking member includes two groups of magnetic members; two groups of mounting holes are provided on the opposite sides of the base plate and the cover plate, and the two groups of magnetic members are respectively arranged in the mounting holes of the base plate and the cover plate, and can be adsorbed on each other to achieve locking and separation of the base plate and the cover plate.
[0012] Furthermore, handle grooves are provided on both sides of the cover plate.
[0013] Furthermore, the bottom plate also has a cavity for placing the cuvette module.
[0014] Furthermore, the cuvette module includes a mounting frame and a cuvette, the mounting frame is installed in the cavity, and the mounting frame has a positioning groove for placing the cuvette.
[0015] Furthermore, a stopper is provided at one end of the cuvette.
[0016] Furthermore, a detection hole groove which is vertically through and facing the colorimetric dish is provided at the bottom of the bottom plate.
[0017] Compared with the prior art, the utility model has the following beneficial effects:
[0018] The enzyme labeling instrument detection plate of the utility model is used to place the microplate in the receiving groove, and then operate the cover plate to press the microplate in the receiving groove to prevent it from moving. The operation is simple, the installation position is stable, and the detection accuracy is improved.
[0019] The enzyme-labeled instrument detection plate of the utility model combines the cuvette module and the microplate together, avoiding the problems caused by large dosage and uneven optical path when using the enzyme-labeled instrument for detection, making the enzyme-labeled instrument detection applicable to more experiments. In addition, the detection position of the plate corresponds to the well position of the 96-well enzyme-labeled plate, and is applicable to most enzyme-labeled instruments on the market. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 This is a schematic diagram of the main structure of the enzyme labeling instrument detection board of the utility model. Figure 1 ;
[0021] Figure 2 This is a schematic diagram of the main structure of the enzyme labeling instrument detection board of the utility model. Figure 2 ;
[0022] Figure 3 This is a schematic diagram of the main structure of the enzyme labeling instrument detection board of the utility model. Figure 3 ;
[0023] Description of Figure Numbers:
[0024] 100, bottom plate; 110, receiving slot; 120, first hole slot; 130, receiving cavity; 140, cavity; 150, detection hole slot;
[0025] 200, cover plate; 210, second hole groove; 220, groove; 230, handle groove;
[0026] 300, microplate; 400, pressing plate; 500, limiting hole; 600, mounting hole; 700, mounting frame; 800, cuvette; 900, pressing plug.
[0027] The realization of the purpose, functional features and advantages of the utility model will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION
[0028] In order to make the purpose, technical solution and beneficial effects of the utility model more clear, the technical solution of the utility model is further described below in conjunction with the drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.
[0029] In the description of the utility model, it should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of the specification are only used to match the contents disclosed in the specification for people familiar with the technology to understand and read, and are not used to limit the limiting conditions that the utility model can be implemented, so they have no substantial technical significance. Any modification of the structure, change of the proportional relationship or adjustment of the size should still fall within the scope of the technical content disclosed by the utility model without affecting the effect that the utility model can produce and the purpose that can be achieved. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the utility model. The change or adjustment of the relative relationship should also be regarded as the scope of the implementation of the utility model without substantial change of the technical content.
[0030] like Figure 1-3 As shown, an embodiment of the utility model provides an ELISA test plate, comprising:
[0031] The bottom plate 100 has a receiving groove 110 for placing the microplate 300. A plurality of first hole grooves 120 are arranged in a rectangular array in the receiving groove 110, and the bottom of each first hole groove 120 is transparent. The size of the bottom plate 100 is the same as that of the existing 96-well ELISA plate, which is convenient for use in ELISA instruments on the market. At the same time, the specific structural shape of the receiving groove 110 is relatively changed according to the structure of the microplate 300, and no limitation is made in the description of this embodiment. For example, in this embodiment, the microplate 300 with a rectangular structure is taken as an example, and the corresponding groove 220 is also a rectangular structure;
[0032] The cover plate 200 is rotatably connected to the base plate 100, and the cover plate 200 is provided with a plurality of second holes 210 in a rectangular array, and the bottom of each second hole 210 is transparent and corresponds one-to-one to the first hole 120, wherein the number of the first hole 120 and the second hole 210 is the same as the detection hole 150 on the microplate 300, and corresponds one-to-one, and of course is also the same as the hole position of the existing 96-well ELISA plate. In this embodiment, there are three first holes 120 and second holes 210 in the horizontal direction and eight in the vertical direction, totaling twenty-four, so that the sample amount can be controlled below 5ul for detection, avoiding a large amount of detection sample; and a locking member, which is used to realize the locking of the base plate 100 and the cover plate 200 toward one side, so as to fasten the microplate 300 in the receiving groove 110.
[0033] When using the enzyme-labeled instrument detection plate of the utility model, the microplate 300 is placed in the receiving groove 110, and then the cover plate 200 is operated to press the microplate 300 in the receiving groove 110 to prevent movement. The operation is simple, the installation position is stable, and the accuracy of the detection is improved.
[0034] like Figure 1-3 As shown, in the embodiment of the utility model, a sealing ring is provided on the microplate 300 for storing samples; a groove 220 is provided on the side of the cover plate 200 facing the bottom plate 100, a pressing plate 400 is provided in the groove 220 and abuts against the sealing ring, and a through hole is provided at the bottom of the pressing plate 400 which is vertically penetrated and faces the second hole groove 210. When in use, the microplate 300 (with samples stored) is placed in the receiving groove 110, and then the pressing plate 400 is placed in the groove 220, and then the cover plate 200 is turned over, and the pressing plate 400 in the groove 220 abuts against the sealing ring of the microplate 300 in the receiving groove 110, thereby preventing the sample from splashing out during movement, and at the same time, the sample can be better formed into a liquid column, which is convenient for subsequent detection and improves detection accuracy.
[0035] like Figure 1-3 As shown, in an embodiment of the utility model, the base plate 100 also has a accommodating cavity 130, the cover plate 200 covers the accommodating cavity 130, and the accommodating groove 110 is arranged in the accommodating cavity 130. When in use, the cover plate 200 is flipped to be located in the accommodating cavity 130 to limit the microplate 300.
[0036] like Figure 1-3 As shown, in the embodiment of the utility model, the accommodating cavity 130 and the cover plate 200 are both provided with a limiting hole 500, and the limiting hole 500 is used to install a limiting member to limit the microporous plate 300 and the pressing plate 400 so that they are firmly fixed in the groove. The limiting member can be an M2 screw, which is convenient for disassembly and installation, and thus convenient for taking the microporous plate 300 and the pressing plate 400.
[0037] like Figure 1-3As shown, in an embodiment of the utility model, the locking member includes two groups of magnetic members; two groups of mounting holes 600 are provided on the opposite sides of the base plate 100 and the cover plate 200, and the two groups of magnetic members are respectively arranged in the mounting holes 600 of the base plate 100 and the cover plate 200, and can be adsorbed on each other to achieve locking and separation of the base plate 100 and the cover plate 200. The number of magnetic members in each group can be two, three or four, etc., and the base plate 100 and the cover plate 200 can be quickly connected together through the magnetic members, making it easier to operate.
[0038] like Figure 1-3 As shown, in the embodiment of the utility model, handle grooves 230 are provided on both sides of the cover plate 200. By putting fingers into the handle grooves 230, the cover plate 200 can be opened easily, which is convenient for operation.
[0039] like Figure 1-3 As shown, in the embodiment of the utility model, the base plate 100 also has a cavity 140 for placing the cuvette 800 module, combining the cuvette 800 module and the microplate 300 together, solving the problems caused by large dosage and uneven optical path when using the microplate reader for detection, making the microplate reader detection applicable to more experiments.
[0040] like Figure 1-3 As shown, in an embodiment of the utility model, the cuvette 800 module includes a mounting frame 700 and a cuvette 800 for fixing the sample optical path. The mounting frame 700 is installed in the cavity 140. The mounting frame 700 has a positioning groove for placing the cuvette 800. The positioning groove may be one or two, etc. At the same time, a plug 900 is provided at one end of the cuvette 800 to reduce the possibility of leakage, reduce the error caused by the optical path, and play a role in fixing the cuvette 800.
[0041] like Figure 1-3 As shown, in the embodiment of the utility model, the bottom of the base plate 100 is provided with a detection hole groove 150 which is vertically through and opposite to the cuvette 800. When in use, the light source vertically passes through the cuvette 800 and the detection hole groove 150 from top to bottom in sequence to perform light transmission detection on the sample in the cuvette 800.
[0042] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.
Claims
1. A microplate reader detection plate, characterized in that: include: The bottom plate (100) has a receiving groove (110) for placing the microplate (300), wherein a plurality of first hole grooves (120) are arranged in a rectangular array in the receiving groove (110), and the bottom of each first hole groove (120) is transparent; A cover plate (200) is rotatably connected to the bottom plate (100), wherein the cover plate (200) is provided with a plurality of second holes (210) in a rectangular array, and the bottom of each second hole (210) is transparent and corresponds to the first hole (120) one by one; and The locking piece is used to lock the bottom plate (100) and the cover plate (200) toward one side, so as to fasten the microporous plate (300) in the containing groove (110).
2. A microplate reader test plate as claimed in claim 1, characterized in that: The microplate (300) is provided with a sealing ring for storing samples; the cover plate (200) has a groove (220) on one side facing the bottom plate (100); a pressing plate (400) is provided in the groove (220) to abut against the sealing ring; and a through hole is provided at the bottom of the pressing plate (400) that penetrates vertically and faces the second hole groove (210).
3. A microplate reader test plate as claimed in claim 2, characterized in that: The bottom plate (100) further comprises a receiving cavity (130), the cover plate (200) covers the receiving cavity (130), and the receiving groove (110) is arranged in the receiving cavity (130).
4. A microplate reader test plate as claimed in claim 3, characterized in that: The accommodating cavity (130) and the cover plate (200) are both provided with a limiting hole (500), and the limiting hole (500) is used to install a limiting member to limit the microporous plate (300) and the pressing plate (400).
5. The ELISA test plate according to claim 1, characterized in that: The locking member comprises two groups of magnetic suction members; two groups of mounting holes (600) are provided on opposite sides of the base plate (100) and the cover plate (200); the two groups of magnetic suction members are respectively arranged in the mounting holes (600) of the base plate (100) and the cover plate (200), and can be mutually adsorbed to achieve locking and separation of the base plate (100) and the cover plate (200).
6. The ELISA test plate according to claim 1, characterized in that: Handle grooves (230) are provided on both sides of the cover plate (200).
7. A microplate reader test plate according to any one of claims 1 to 6, characterized in that: The base plate (100) also has a cavity (140) for placing a cuvette (800) module.
8. A microplate reader test plate as claimed in claim 7, characterized in that: The cuvette (800) module comprises a mounting frame (700) and a cuvette (800), wherein the mounting frame (700) is installed in the cavity (140), and the mounting frame (700) has a positioning groove for placing the cuvette (800).
9. A microplate reader test plate as claimed in claim 8, characterized in that: A stopper (900) is provided at one end of the cuvette (800).
10. A microplate reader test plate as claimed in claim 8 or 9, characterized in that: The bottom of the bottom plate (100) is provided with a detection hole groove (150) which is vertically penetrated and faces the colorimetric dish (800).