Anterior chamber perfusion device for batch preparation of acute intraocular hypertension animal models

Abstract

The utility model relates to the technical field of animal experiment devices, and discloses an anterior chamber perfusion device for batch preparation of acute intraocular hypertension animal models, which comprises a base arranged on the ground, an operation table and a supporting piece arranged on the surface of one side, far away from the ground, of the base and perpendicular to the base; the sterile normal saline bag is arranged on the supporting piece, the sterile normal saline bag and the ground are spaced by a certain distance through the supporting piece, and normal saline is contained in the sterile normal saline bag; and the disposable infusion apparatus is arranged on the sterile normal saline bag and is connected with the sterile normal saline bag. According to the anterior chamber perfusion device for preparing the acute intraocular hypertension animal models in batches, the experiment accuracy is improved, and on the whole, the device provides an efficient, accurate and low-resource-consumption ideal tool for preparing the acute intraocular hypertension animal models in biomedical research.

Description

Technical Field

[0001] The utility model relates to the technical field of animal experimental devices, in particular to an anterior chamber perfusion device for batch preparation of acute high intraocular pressure animal models. Background Art

[0002] In biomedical research, creating a stable animal model of acute ocular hypertension is crucial for gaining a deeper understanding of the pathophysiological mechanisms of ocular hypertension-related diseases. Currently, a commonly used method is anterior chamber perfusion, which usually involves inserting a 30G needle into the anterior chamber of the animal from the level of the corneal limbus and connecting it to a sterile saline infusion system at a specific height to continuously perfuse saline for up to one hour. However, this "one-to-one" (i.e., one needle and one infusion system for one animal) operation mode has exposed some significant defects in practice.

[0003] First, from the perspective of modeling efficiency, this method has significant limitations. Since the entire modeling process requires continuous perfusion of saline for up to one hour, and during this process, the position of the needle must be closely monitored to ensure that it does not loosen or accidentally fall out of the anterior chamber, this delicate operation not only increases the complexity of modeling, but also makes the modeling process lengthy and inefficient. In scientific research, the preciousness of time is self-evident. Therefore, improving modeling efficiency has become an urgent problem to be solved. Secondly, from the perspective of model consistency, the anterior chamber perfusion method also faces challenges. During the perfusion process, the level of intraocular pressure of the animal directly depends on the height difference between the saline liquid level and the animal eye plane. However, since the saline liquid level of different infusion systems may undergo different height changes during the perfusion process, this leads to differences in intraocular pressure between different animal models. This difference not only affects the comparability of the model, but may also mislead the experimental results. Therefore, ensuring the consistency of intraocular pressure between different models has become another urgent problem to be solved. Utility Model Content

[0004] 1. Technical issues to be resolved

[0005] The utility model aims to solve the above problems and proposes an anterior chamber perfusion device for batch preparation of acute high intraocular pressure animal models.

[0006] (II) Technical solution

[0007] The technical solution of the utility model to solve the above technical problems is as follows:

[0008] An anterior chamber perfusion device for batch preparation of acute high intraocular pressure animal models comprises a base placed on the ground and an operating table.

[0009] The support member is arranged on a side surface of the base away from the ground, and is arranged perpendicular to the base;

[0010] A sterile saline bag is arranged on a support, and the support separates the sterile saline bag from the ground to a certain distance, wherein the sterile saline bag contains saline;

[0011] A disposable infusion set is arranged on the sterile saline bag and is connected to the sterile saline bag;

[0012] A shunt device is disposed on the other end of the disposable infusion set and is in communication with the disposable infusion set, wherein the shunt device is provided with a plurality of outlets, and the shunt device is used to allow the liquid medicine in the sterile physiological saline bag to flow out from the plurality of outlets;

[0013] Transparent silicone capillaries are arranged on the flow-dividing device and are interconnected with the flow-dividing device, and the number and distribution positions of the transparent silicone capillaries are adapted to the outlet of the flow-dividing device;

[0014] The insulin needle is arranged on the other end of the transparent silicone capillary and is communicated with the transparent silicone capillary, and is used for penetrating into the anterior chamber of the animal's eye.

[0015] On the basis of the above technical solution, the present invention can also be improved as follows.

[0016] Furthermore, the support member is an adjustable infusion pole, wherein a hook seat is fixedly installed at one end of the support member away from the base, a hook member is fixedly installed at the edge of the hook seat, and a number of hook members are set, which can be symmetrically distributed according to the center of the hook seat, and a sterile saline bag is hung on the hook member.

[0017] Furthermore, the shunt device includes:

[0018] A main flow tube, one end of which is an inflow end and the other end is an outflow end, wherein the inflow end is connected to an end of the disposable infusion set away from the sterile saline bag;

[0019] A plurality of branch flow pipes are provided and symmetrically distributed outside the main flow pipe, wherein the end of the branch flow pipe away from the main flow pipe is the outflow end; and

[0020] The valves are arranged on the main flow pipe and the branch flow pipe, the total number of which is the same as the sum of the number of the inflow end and the outflow end, and the distribution positions thereof are matched one by one with the inflow end and the outflow end.

[0021] Furthermore, the transparent silicone capillaries are arranged on each outflow end, and the number and distribution positions of the transparent silicone capillaries are matched with the outflow ends.

[0022] Furthermore, a heat preservation pad is pasted on the surface of the operating table on one side away from the ground.

[0023] (III) Beneficial effects

[0024] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0025] The anterior chamber perfusion device for batch preparation of acute ocular hypertension animal models, with its unique design and functionality, realizes the simultaneous perfusion of multiple animal models, significantly improves the modeling efficiency, and ensures the consistency of intraocular pressure between different models. The use of highly adjustable supports, precisely controlled valves, disposable infusion sets and transparent silicone capillaries not only simplifies the operation process but also reduces the experimental cost. In addition, the setting of the warming pad ensures that the animal body temperature is maintained constant during the perfusion process, thereby improving the accuracy of the experiment. Overall, the device provides an ideal tool for the preparation of acute ocular hypertension animal models in biomedical research that is efficient, accurate and resource-efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the overall connection structure of the utility model;

[0027] Figure 2 For this utility model Figure 1 Enlarged view of point A in the middle.

[0028] In the figure: 1. base; 2. operating table; 3. support; 4. sterile saline bag; 5. disposable infusion set; 6. shunt device; 61. main flow tube; 62. branch flow tube; 63. valve; 7. transparent silicone capillary; 8. insulin needle; 9. hook seat; 10. hook piece; 11. thermal pad. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0030] Combination Figure 1-Figure 2 As shown, the utility model is an anterior chamber perfusion device for batch preparation of acute high intraocular pressure animal models, comprising a base 1 placed on the ground and an operating table 2.

[0031] The support member 3 is arranged on a side surface of the base 1 away from the ground, and is perpendicular to the base 1;

[0032] A sterile saline bag 4 is disposed on the support 3, and the support 3 separates the sterile saline bag 4 from the ground to a certain distance, wherein the sterile saline bag 4 contains saline;

[0033] A disposable infusion set 5 is disposed on the sterile saline bag 4 and is connected to the sterile saline bag 4;

[0034] The shunt device 6 is disposed on the other end of the disposable infusion set 5 and is in communication with the disposable infusion set 5, wherein the shunt device 6 is provided with a plurality of outlets, and the shunt device 6 is used to allow the liquid medicine in the sterile saline bag 4 to flow out from the plurality of outlets;

[0035] Transparent silicone capillaries 7 are disposed on the flow dividing device 6 and are interconnected with the flow dividing device 6, and the number and distribution positions of the transparent silicone capillaries 7 are adapted to the outlet of the flow dividing device 6;

[0036] The insulin needle 8 is disposed on the other end of the transparent silicone capillary 7 and is communicated with the transparent silicone capillary 7 for penetrating into the anterior chamber of the eye of the animal.

[0037] The device is mainly composed of a base 1 and an operating table 2, wherein the base 1 is stably placed on the ground, and a support 3 is vertically arranged on the side surface of the base 1 away from the ground to support and fix the upper components. A sterile saline bag 4 is placed on the support 3 and keeps a certain distance from the ground through the support 3. The sterile saline bag 4 is filled with saline or medicine for infusion, and a disposable infusion set 5 is connected to the sterile saline bag 4. One end of the disposable infusion set 5 is connected to the outlet of the sterile saline bag 4, and the other end is connected to the shunt device 6. The disposable infusion set 5 is responsible for transporting the medicine in the sterile saline bag 4 to the shunt device 6. The shunt device 6 is the core component of the device, and multiple outlets are arranged on it. The medicine in the disposable infusion set 5 enters through the inflow end of the shunt device 6, and is shunted inside the shunt device 6, and finally flows out from multiple outlets, each outlet The outlet of each of the two eye sockets is connected to a transparent silicone capillary 7, the number and distribution position of the transparent silicone capillary 7 are adapted to the outlet of the shunt device 6, the other end of the transparent silicone capillary 7 is connected to an insulin needle 8, the insulin needle 8 has sufficient fineness and flexibility, and can be easily penetrated into the anterior chamber of the animal's eye. During operation, the insulin needle 8 is inserted into the anterior chamber of the animal, thereby establishing a passage from the sterile saline bag 4 to the anterior chamber of the animal. When the disposable infusion device 5 is started, the medicine in the sterile saline bag 4 will, under the action of pressure, pass through the disposable infusion device 5, the shunt device 6, the transparent silicone capillary 7 and the insulin needle 8, and finally flow into the anterior chamber of the animal, thereby realizing anterior chamber perfusion, and then preparing an acute high intraocular pressure animal model, by controlling the opening and closing of the valve on the shunt device 6, the accurate control of the anterior chamber perfusion amount of each animal model can be achieved, ensuring the consistency between different models.

[0038] In a preferred embodiment, the present invention can be further configured as follows: Figure 1 , Figure 2 As shown; the support member 3 is an adjustable infusion pole, wherein a hook seat 9 is fixedly installed at one end of the support member 3 away from the base, and a hook member 10 is fixedly installed at the edge of the hook seat 9. There are several hook members 10, which can be symmetrically distributed according to the center of the hook seat 9. The sterile saline bag 4 is hung on the hook member 10, and the specific design of the support member 3 is further clarified, that is, the support member 3 is an adjustable infusion pole, and is equipped with a hook seat 9 and a hook member 10 to hang the sterile saline bag 4. The support member 3, as an important part of the entire device, is designed as an adjustable infusion pole, which means that its length and angle can be adjusted. This design allows the height and position of the sterile saline bag 4 to be flexibly adjusted according to experimental needs. A hook seat 9 is fixedly installed at one end of the support member 3 away from the base 1. The hook seat 9 serves as a fixed base for the hook member 10 to ensure the stability and reliability of the hook member 10. Several hook members 10 are fixedly installed at the edge of the hook seat 9, and these hook members 10 are symmetrically distributed according to the center of the hook seat 9. The distribution ensures that the sterile saline bag 4 hung thereon is subjected to uniform force, and prevents shaking or falling off due to the shift of the center of gravity. The sterile saline bag 4 is hung on the hook part 10 through its own suspension structure or additional fixing device. Since the number and distribution of the hook parts 10 are designed according to actual needs, multiple sterile saline bags 4 can be hung at the same time to meet the needs of batch preparation of acute high intraocular pressure animal models. When the height or position of the sterile saline bag 4 needs to be adjusted, it can be achieved by adjusting the length or angle of the support part 3. This flexible adjustability allows the experimenter to accurately control the height and position of the sterile saline bag 4 according to the body shape, position and other factors of the experimental animal, thereby ensuring the accuracy and safety of anterior chamber perfusion. In summary, by designing an adjustable support part 3 and equipping a hook seat 9 and a hook part 10, a stable and reliable suspension method is provided for the sterile saline bag 4, and a high degree of flexible adjustability is achieved, which provides strong support for the batch preparation of acute high intraocular pressure animal models.

[0039] In a preferred embodiment, the present invention can be further configured as follows: Figure 1 , Figure 2 As shown; the shunt device 6 includes:

[0040] A main flow tube 61, one end of which is an inflow end and the other end of which is an outflow end, wherein the inflow end is connected to an end of the disposable infusion set 5 away from the sterile saline bag 4;

[0041] A plurality of branch flow pipes 62 are provided and symmetrically distributed outside the main flow pipe 61, wherein the end of the branch flow pipe 62 away from the main flow pipe 61 is an outflow end; and

[0042] The valve 63 is arranged on the main flow tube 61 and the branch flow tube 62. The total number of valves 63 is the same as the sum of the number of the inflow end and the outflow end. The distribution position of valves 63 is matched with the inflow end and the outflow end one by one. The structure and working principle of the shunt device 6 are described in detail, aiming to achieve simultaneous perfusion of multiple animal models and ensure that the amount and pressure of physiological saline received by each animal are consistent. The shunt device 6 is a key component in the anterior chamber perfusion device. Its main function is to distribute the physiological saline or medicine delivered from the disposable infusion set 5 to different branch flow tubes 62, so as to achieve simultaneous perfusion of multiple animal models. The shunt device 6 consists of several main parts: the main flow tube 61, the branch flow tube 62 and the valve 63. The main flow tube 61 is connected to the branch flow tube 62, and the valve 63 is connected to the branch flow tube 62. Through tube 61: One end of the main flow tube 61 is used as the inflow end, and is interconnected with the end of the disposable infusion set 5 away from the sterile saline bag 4. When the disposable infusion set 5 starts to work, the medicine in the sterile saline bag 4 will flow into the inflow end of the main flow tube 61 through the disposable infusion set 5. The other end of the main flow tube 61 is the outflow end, but it is not directly used for perfusing the animal model, but serves as a gathering point for the branch flow tubes 62. The branch flow tubes 62: The number of branch flow tubes 62 is set according to the experimental needs. Usually, there are multiple branch flow tubes, which are symmetrically distributed on the outside of the main flow tube 61. The end of each branch flow tube 62 away from the main flow tube 61 is the outflow end. These outflow ends are respectively connected to the transparent silicone capillary 7 , and then connected to the insulin needle 8, for anterior chamber perfusion to different animal models, valve 63: valve 63 is a key component for controlling the flow of liquid. Valves 63 are provided on the main flow tube 61 and each branch flow tube 62. The total number of valves 63 is the same as the sum of the number of inflow ends and outflow ends, and the distribution position is matched with the inflow end and the outflow end one by one. By adjusting the valve 63, the experimenter can control the flow rate and flow velocity of the liquid in each branch flow tube 62, so as to ensure that the amount and pressure of physiological saline received by each animal are consistent. When the experiment starts, firstly, by adjusting the height of the support member 3 and the hook member 10, the sterile physiological saline bag 4 is placed in an appropriate position, and then, a start is made. The medicine in the sterile saline bag 4 flows into the main flow tube 61 through the disposable infusion set 5. In the main flow tube 61, the medicine is distributed to each branch flow tube 62. By adjusting the valve 63, the liquid flow and flow rate in each branch flow tube 62 can be controlled to ensure that each animal receives the same amount and pressure of saline. Finally, the medicine flows into the anterior chamber of the animal through the transparent silicone capillary 7 and the insulin needle 8 to achieve anterior chamber perfusion. This design allows multiple animal models to be perfused at the same time, greatly improving the experimental efficiency. The valve 63 is adjusted to ensure the consistency of the amount and pressure of saline received by each animal, thereby improving the accuracy and reliability of the experiment.

[0043] In a preferred embodiment, the present invention can be further configured as follows: Figure 1 , Figure 2 As shown; transparent silicone capillaries 7 are arranged on each outflow end, and their number and distribution position are adapted to the outflow end. Transparent silicone capillaries 7 are an important component of the anterior chamber perfusion device for batch preparation of acute high intraocular pressure animal models. It is directly related to the delivery of physiological saline from the shunt device 6 to the anterior chamber of the animal's eye. The transparent silicone capillaries 7 serve as an important channel connecting the shunt device 6 and the anterior chamber of the animal's eye. Its design ensures the stable flow and accurate delivery of physiological saline. In the shunt device 6, the outflow end of the branch flow tube 62 serves as the outlet of the physiological saline. Each outflow end is equipped with a corresponding number and position of transparent silicone capillaries 7. The number and distribution position of the transparent silicone capillaries 7 are adapted to the outflow end of the branch flow tube 62, which means that each outflow end has one or more transparent silicone capillaries 7 connected to it. Such a design ensures that each animal model Each model can receive saline solution through an independent channel, thus avoiding cross contamination or interference between different models. During operation, when the disposable infusion set 5 is started, the saline solution in the sterile saline bag 4 enters the main flow tube 61 of the shunt device 6 through the disposable infusion set 5, and then is shunted to each branch flow tube 62. At the outflow end of the branch flow tube 62, the saline solution flows into the corresponding transparent silicone capillary 7. Since the transparent silicone capillary 7 is soft, transparent and durable, it can be flexibly inserted into the anterior chamber of the animal and ensure that the saline solution flows in accurately and stably. In addition, the transparency of the transparent silicone capillary 7 enables the experimenter to clearly observe the flow of saline solution in the tube, which helps to promptly discover and solve problems. At the same time, the silicone material has good biocompatibility and will not cause additional harm or discomfort to the animal.

[0044] In a preferred embodiment, the present invention can be further configured as follows: Figure 1 , Figure 2As shown; a heat preservation pad 11 is pasted on the surface of the side of the operating table 2 away from the ground. This design is intended to maintain the stability of the experimental environment on the operating table, especially in temperature-sensitive experimental processes, such as the preparation of animal models. In the process of batch preparation of acute high intraocular pressure animal models, the physiological state of the animal models is often sensitive to temperature. Therefore, maintaining the temperature stability of the experimental environment is crucial to the accuracy and reliability of the experiment. The operating table 2 is the main working area of ​​the experiment, and the control of its surface temperature is particularly important. The heat preservation pad 11 is pasted on the surface of the side of the operating table 2 away from the ground. Its main function is to reduce the heat exchange between the surface of the operating table and the external environment, thereby keeping the temperature of the surface of the operating table relatively stable. The heat preservation pad 11 is usually made of a material with good thermal insulation performance, such as foam plastic, rubber Glue, etc., these materials can effectively insulate heat and prevent heat loss or external heat intrusion. During the experiment, the thermal insulation pad 11 can ensure that the surface temperature of the operating table 2 remains constant within a certain range, which is of great significance for maintaining the physiological state of the animal model and ensuring the accuracy of the experimental results. For example, when preparing an acute high intraocular pressure animal model, the thermal insulation pad 11 can prevent the instability of the physiological state of the animal model caused by the operating table temperature being too low or too high, thereby affecting the accuracy of the experimental results and maintaining the constant body temperature of the animal. In addition, the thermal insulation pad 11 also has anti-slip, shock-absorbing and other functions, which can protect the experimental equipment and animal models on the operating table from damage. At the same time, its soft properties can also provide a comfortable working environment for the experimenters and reduce the fatigue caused by long-term work.

[0045] In biomedical research, in order to efficiently and accurately prepare an acute high intraocular pressure animal model to evaluate the therapeutic effect and drug response of ophthalmic diseases, we designed a special anterior chamber perfusion device. The device is based on a base 1, and the height and angle are adjusted by an adjustable support 3 to meet different experimental operation requirements. The sterile saline bag 4 is hung on the hook 10 of the support 3 and connected to the shunt device 6 through a disposable infusion set 5 to ensure a stable supply of medicine. The shunt device 6 consists of a main flow tube 61 and a plurality of branch flow tubes 62. Each branch flow tube 62 is provided with a valve 63, which can independently control the liquid flow and flow rate to ensure that the amount and pressure of saline received by each animal are consistent. The transparent silicone capillary 7 is connected to the outflow end of the branch flow tube 62. Its soft and transparent characteristics facilitate the observation of the flow of medicine. The insulin needle 8 is connected to the other end of the transparent silicone capillary 7 for precise penetration into the anterior chamber of the animal's eye to achieve medicine perfusion. At the same time, a heat preservation pad 11 is provided on the operating table 2 to maintain a stable temperature and ensure the consistency of the experimental environment.

[0046] In summary, the device achieves simultaneous perfusion of multiple animal models through the coordinated use of key components, improves modeling efficiency and accuracy, reduces experimental costs, and provides an ideal tool for biomedical research that is efficient, accurate, and consumes less resources.

[0047] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0048] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An anterior chamber perfusion device for batch preparation of acute intraocular hypertension animal models, comprising a base (1) placed on the ground and an operating table (2), characterized in that: The support member (3) is arranged on a side surface of the base (1) away from the ground, and is arranged perpendicular to the base (1); A sterile saline bag (4) is arranged on the support (3), and the support (3) separates the sterile saline bag (4) from the ground at a certain distance, wherein the sterile saline bag (4) contains saline; A disposable infusion set (5) is arranged on the sterile saline bag (4) and is connected to the sterile saline bag (4); A shunt device (6) is arranged on the other end of the disposable infusion set (5) and is in communication with the disposable infusion set (5), wherein the shunt device (6) is provided with a plurality of outlets, and the shunt device (6) is used to allow the liquid medicine in the sterile physiological saline bag (4) to flow out from the plurality of outlets; Transparent silica gel capillaries (7) are arranged on the flow dividing device (6) and are in communication with the flow dividing device (6), and the number and distribution positions of the transparent silica gel capillaries (7) are adapted to the outlet of the flow dividing device (6); The insulin needle (8) is arranged on the other end of the transparent silicone capillary (7) and is communicated with the transparent silicone capillary (7) for penetrating into the anterior chamber of the eye of the animal.

2. The anterior chamber perfusion device for batch preparation of acute intraocular hypertension animal models according to claim 1, characterized in that: The support member (3) is an adjustable infusion pole, wherein a hook seat (9) is fixedly mounted on one end of the support member (3) away from the base, a hook member (10) is fixedly mounted on the edge of the hook seat (9), and the number of hook members (10) is set to be symmetrically distributed according to the center of the hook seat (9), and the sterile physiological saline bag (4) is hung on the hook member (10).

3. The anterior chamber perfusion device for batch preparation of acute intraocular hypertension animal models according to claim 1, characterized in that: The shunt device (6) comprises: A main flow tube (61), one end of which is an inflow end and the other end of which is an outflow end, wherein the inflow end is in communication with an end of the disposable infusion set (5) away from the sterile physiological saline bag (4); A plurality of branch flow pipes (62) are provided and symmetrically distributed outside the main flow pipe (61), wherein the end of the branch flow pipe (62) away from the main flow pipe (61) is an outflow end; and The valves (63) are arranged on the main flow pipe (61) and the branch flow pipe (62). The total number of the valves is the same as the total number of the inflow ends and the outflow ends, and the distribution positions thereof are matched one by one with the inflow ends and the outflow ends.

4. The anterior chamber perfusion device for batch preparation of acute intraocular hypertension animal models according to claim 3, characterized in that: The transparent silica gel capillaries (7) are arranged on each outflow end, and their number and distribution positions are adapted to the outflow ends.

5. The anterior chamber perfusion device for batch preparation of acute intraocular hypertension animal models according to claim 1, characterized in that: A heat-insulating pad (11) is adhered to the surface of the operating table (2) on one side away from the ground.

Images