Collecting device for liquid concentrated growth factor CGF
By designing a collection device including a baffle, a height adjustment assembly and a Basp pipette, the problem of red blood cell suspension caused by poor hand stability of the operator is solved, and the precise collection of liquid CGF and the improvement of product quality is achieved.
Patent Information
- Application Number
- CN202421699496.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-17
- Publication Date
- 2025-05-09
- Estimated Expiration
- 2034-07-17
AI Technical Summary
During the process of collecting liquid concentrated growth factor CGF, poor hand stability of the operator can easily lead to red blood cell suspension, affecting product quality.
A collection device including a baffle, a height adjustment assembly and a Bapuerk straw is designed. The suction position of the Bapuerk straw is adjusted through the height adjustment assembly to accurately control the suction depth and avoid hand-held shaking.
Accurate collection of liquid CGF is achieved, reducing the possibility of red blood cell suspension, and improving product quality and operating stability.
Smart Images
Figure CN222842282U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to a collection device, and in particular to a collection device for liquid concentrated growth factor CGF. Background Art
[0002] Concentrate Growth Factors (CGF) is a mixture of PRP, CD34+ cells, leukocytes, and concentrated growth factors obtained by centrifugation of autologous venous blood into anticoagulation tubes. It is rich in growth factors, leukocytes, CD34+ cells, and fibrin, and has better ability to promote bone tissue, soft tissue, and skin regeneration. Whether it contains CD34+ positive cells is an important sign that distinguishes CGF from PRP.
[0003] In the prior art, a variable speed centrifuge is used, a blood collection tube containing an anticoagulant is placed, and the "CGF" mode is selected for centrifugation. After the centrifugation is completed, liquid CGF can be prepared. However, during the collection process, the operator needs to use a Pasteur pipette to reach into the blood collection tube and scrape off the "half-moon-shaped" white film layer rich in CGF (PRP and CD34+ cells) and red blood cells attached to the tube wall, which is the liquid concentrated growth factor CGF. During the operation of absorbing the white film layer rich in CD34+ cells, the operator's hands need to be very stable. If there is too much shaking during the operation, it is easy to cause shaking, resulting in red blood cell suspension. Once the red blood cell suspension is caused, too many red blood cells will affect the use effect of the product. However, during long-term operation, it is easy to cause the operator's hand fatigue, affecting the stability and accuracy of the collection. Utility Model Content
[0004] The present application provides a device for collecting liquid concentrated growth factor CGF to solve the problems existing in the related art. The technical solution is as follows:
[0005] The present application embodiment provides a device for collecting liquid concentrated growth factor CGF, comprising:
[0006] A baffle, which is detachably arranged on the centrifuge tube and has a through hole;
[0007] A height adjustment component, the height adjustment component is disposed on the baffle through the through hole, and the height adjustment component moves relative to the baffle;
[0008] A Pasteur pipette, one end of which passes through the height adjustment component and extends into the interior of the centrifuge tube. The depth of one end of the Pasteur pipette located inside the centrifuge tube is controlled by adjusting the position of the height adjustment component relative to the baffle.
[0009] In one embodiment,
[0010] An inner groove matched with the wall of the centrifuge tube is arranged on the baffle, and the inner groove is communicated with the through hole.
[0011] In one embodiment,
[0012] The baffle is provided with threads, the threads are located on the inner surface of the through hole, and the baffle is threadedly connected with the height adjustment component through the threads.
[0013] In one embodiment,
[0014] Height adjustment kit includes:
[0015] The spiral block is threadably connected with the baffle plate through a second thread, and one end of the Pasteur pipette penetrates the spiral block and extends into the centrifuge tube.
[0016] In one embodiment,
[0017] The height adjustment kit also includes:
[0018] The handle is arranged on one end of the spiral block located outside the centrifuge tube.
[0019] In one embodiment,
[0020] The Pasteur pipette has a sealing balloon on one end that is located outside the centrifuge tube.
[0021] In one embodiment,
[0022] The diameter of the inner groove is larger than the diameter of the through hole.
[0023] In one embodiment,
[0024] The size of the inner groove is matched with the size of the centrifuge tube mouth.
[0025] In one embodiment,
[0026] The spiral block is provided with a hole for the Pasteur pipette to pass through, and the size of the hole is smaller than the size of the sealing air bag so that the Pasteur pipette can be fixed on the spiral block.
[0027] The advantages or beneficial effects of the above technical solution include at least:
[0028] By setting the height adjustment component, the height of the Pasteur pipette's suction port in the centrifuge tube is adjusted, and the device is adjusted according to the height of the buffy coat layer rich in CD34+ cells after the second centrifugation, so that the platelet-rich plasma (PRP) and the buffy coat layer rich in CD34+ cells after centrifugation are collected. By setting the height adjustment component, the collection depth is made more precise, and the problem of red blood cell suspension caused by manual shaking of the Pasteur pipette can be avoided, so that the liquid concentrated growth factor CGF containing only a small amount of red blood cells can be better extracted.
[0029] The above summary is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the present application will be readily apparent by reference to the accompanying drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS
[0030] In the accompanying drawings, unless otherwise specified, the same reference numerals throughout the multiple drawings represent the same or similar parts or elements. These drawings are not necessarily drawn to scale. It should be understood that these drawings only depict some embodiments disclosed in the present application and should not be regarded as limiting the scope of the present application.
[0031] Figure 1 It is a schematic diagram of the structure of the utility model;
[0032] Figure 2 for Figure 1 A schematic diagram of the structure connected to a centrifuge tube;
[0033] Figure 3 for Figure 2 Schematic diagram of the internal cross-sectional structure;
[0034] Figure 4 A schematic diagram of the structure of the utility model when viewed from above;
[0035] Figure 5 for Figure 4 A schematic diagram of the structure of the middle baffle;
[0036] In the figure: 100, collecting device;
[0037] 110, baffle; 111, through hole; 112, inner groove;
[0038] 120. height adjustment assembly; 121. screw block; 122. handle;
[0039] 130. Pasteur straw; 131. Sealed air bag;
[0040] 140. Centrifuge tube. DETAILED DESCRIPTION
[0041] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the present application. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.
[0042] Figure 1 - Figure 5FIG. 1 is a structural diagram of a collection device 100 for liquid concentrated growth factor CGF according to an embodiment of the present application. Figure 1 - Figure 5 As shown, the collecting device 100 may include:
[0043] The present application embodiment provides a collection device 100 for liquid concentrated growth factor CGF, comprising:
[0044] The baffle 110 is detachably disposed on the centrifuge tube 140 and has a through hole 111;
[0045] A height adjustment component 120, the height adjustment component 120 is disposed on the baffle 110 through the through hole 111, and the height adjustment component 120 moves relative to the baffle 110;
[0046] The Pasteur pipette 130 has one end that passes through the height adjustment assembly 120 and extends into the centrifuge tube 140 . The depth of one end of the Pasteur pipette 130 in the centrifuge tube 140 is controlled by adjusting the position of the height adjustment assembly 120 relative to the baffle 110 .
[0047] In this embodiment, all the collection tubes containing anticoagulants are symmetrically balanced, placed in a special variable speed centrifuge, the "CGF" program is selected, and the start button is pressed to start centrifugation. If a common centrifuge is used, centrifugation is performed according to the following parameters: 2700rpm, 2min→2400rpm, 4min→2700rpm, 4min→3300rpm, 3min. After centrifugation, three parts of platelet-poor plasma (PPP) / concentrated growth factor CGF (containing PRP and CD34+ cells) / red blood cell sediment (RBC) can be separated. The platelet-poor plasma (PPP) / concentrated growth factor CGF (containing PRP and CD34+ cells) / red blood cell sediment (RBC) after centrifugation are arranged in layers from top to bottom, wherein the "half-moon-shaped" white film layer rich in CGF (PRP and CD34+ cells) and red blood cells attached to the tube wall are scraped off and collected in a 15ml centrifuge tube 140, and then the red blood cell removal process is performed at 200g for 5min. After centrifugation, three layers are presented, which are a yellowish liquid rich in PRP, a white film layer rich in CD34+ cells, and red blood cells from top to bottom. At this time, it is necessary to collect the yellowish liquid rich in PRP and the white film layer rich in CD34+ cells, which is the liquid concentrated growth factor CGF. First, use a Pasteur pipette 130 to absorb the plasma layer rich in PRP after centrifugation into a new 15ml centrifuge tube 140, take out the collection device 100, including the baffle 110, the height adjustment component 120 and the Pasteur pipette 130 from the sterile packaging bag, align the baffle 110 with the opening of the centrifuge tube 140, rotate the height adjustment component 120, and fine-tune the distance between the suction port of the Pasteur pipette 130 and the white film layer through the height adjustment component 120, so that the suction port of the Pasteur pipette 130 is located above the white film layer and as close to the white film layer as possible. After the position is rotated and positioned, the hand grasps the top of the Pasteur pipette 130, lifts it up, presses it, so that a negative pressure state is formed inside the Pasteur pipette 130, and then extends it into the centrifuge tube 140, and the hand controls the strength and speed of squeezing the top of the Pasteur pipette 130, and the white film layer rich in CD34+ stem cells above the red blood cells is sucked into the 15ml centrifuge tube 140, and the CGF product rich in PRP and CD34+ stem cells with most of the red blood cells removed is obtained, which has an orange or pink suspension appearance, and there may be a small amount of residual red blood cells, but a small amount of red blood cells will not affect the quality of the product, until the white film layer is completely adsorbed;
[0048] Through the setting of the height adjustment component 120, the height of the position of the suction port of the Pasteur pipette 130 in the centrifuge tube 140 is adjusted, and three layers are presented after the second centrifugation, from top to bottom, respectively, a light yellow liquid rich in PRP, a white film layer rich in CD34+ cells, and red blood cells. According to the height of the white film layer of CD34+ cells, the light yellow liquid rich in PRP and the white film layer rich in CD34+ cells after centrifugation are collected. Through the setting of the height adjustment component 120, the depth of collection is made more accurate, and the problem of red blood cell suspension caused by shaking of the Pasteur pipette 130 manually can be avoided;
[0049] It should be noted that the capacity of the centrifuge tube 140 is 15 ml, and the outside of the centrifuge tube 140 has a scale, and the suction port of the Pasteur pipette 130 can be finely adjusted by the scale;
[0050] When the platelet-rich plasma (PRP) of the top layer is collected, the integrated device 100 of the present invention can be taken out to collect the concentrated growth factor CGF (containing PRP and CD34+ cells) of the bottom layer, and the buffy coat layer rich in CD34+ cells can be accurately absorbed to complete the enrichment of CGF.
[0051] like Figure 1 - Figure 5 As shown, in one embodiment,
[0052] The baffle plate 110 has threads, and the threads are located on the inner surface of the through hole 111 . The baffle plate 110 is threadedly connected to the height adjustment assembly 120 via the threads.
[0053] In this embodiment, the height adjustment component 120 is threadedly connected to the baffle 110. By turning the handle 122 to rotate the height adjustment component 120, the relative position between the height adjustment component 120 and the baffle 110 can be changed, thereby adjusting the length of the Pasteur pipette 130 on the height adjustment component 120 extending into the centrifuge tube 140, thereby accurately controlling the collection of the stratified PRP-rich light yellow liquid and the CD34+ cell-rich white film layer, and ensuring the accuracy of the collection to avoid mixing and cross contamination.
[0054] like Figure 1 , Figure 4 - Figure 5 As shown, in one embodiment,
[0055] The baffle 110 is provided with an inner groove 112 matched with the wall of the centrifuge tube, and the inner groove 112 is connected with the through hole 111 .
[0056] In this embodiment, the baffle 110 is detachably connected to the mouth of the centrifuge tube 140 through the inner groove 112, so as to facilitate the engagement and installation between the baffle 110 and the centrifuge tube 140;
[0057] Furthermore, the size of the inner groove 112 is compatible with the size of the mouth of the centrifuge tube 140 , and the diameter of the inner groove 112 is greater than the diameter of the through hole 111 .
[0058] like Figure 1 - Figure 4 As shown, in one embodiment,
[0059] The height adjustment assembly 120 includes:
[0060] The spiral block 121 is threadably connected to the baffle 110 via a second thread. One end of the Pasteur pipette 130 passes through the spiral block 121 and extends into the centrifuge tube 140 .
[0061] In this embodiment, the spiral block 121 is rotatably connected to the baffle 110 through a thread, and one end of the Pasteur pipette 130 with a suction port passes through the spiral block 121 and extends into the centrifuge tube 140. The distance between the spiral block 121 and the baffle 110 can be changed by rotating the spiral block 121, so that the depth of the suction port of the Pasteur pipette 130 penetrating into the centrifuge tube 140 can be adjusted according to the position of the buffy coat layer rich in CD34+ cells after centrifugation, so that the depth of the suction port of the Pasteur pipette 130 penetrating into the centrifuge tube 140 can be more accurately controlled, while ensuring stability during the process of collecting the buffy coat layer.
[0062] like Figure 1 , Figure 2 and Figure 4 As shown, in one embodiment,
[0063] The height adjustment assembly 120 further includes:
[0064] The handle 122 is disposed on one end of the spiral block 121 located outside the centrifuge tube 140 .
[0065] In this embodiment, the handle 122 is arranged on the spiral block 121 to facilitate the rotation of the spiral block 121. When the depth of the mouth of the Pasteur pipette 130 needs to be adjusted, the handle 122 is used to rotate the spiral block 121 to adjust the distance between the mouth of the Pasteur pipette 130 and the white film layer. While rotating the spiral sleeve, the mouth of the Pasteur pipette 130 is observed to make the mouth of the Pasteur pipette 130 located above the white film layer and as close to the white film layer as possible.
[0066] After the position is rotated and positioned, the top of the Pasteur pipette 130 is grasped by hand, lifted and pressed to form a negative pressure state inside the Pasteur pipette 130, and then inserted into the 15 ml centrifuge tube 140 and fixed;
[0067] Then, the force and speed of squeezing the top of the Pasteur pipette 130 by hand are controlled so that the white film layer can be smoothly sucked into the Pasteur pipette 130 until the white film layer is completely absorbed.
[0068] like Figure 1-Figure 4 As shown, in one embodiment,
[0069] The Pasteur pipette 130 has a sealing air bag 131 on one end located outside the centrifuge tube 140 .
[0070] The spiral block 121 has a hole for the Pasteur straw 130 to pass through. The size of the hole is smaller than the size of the sealing airbag 131 and can fit the body of the Pasteur straw 130 so that the Pasteur straw 130 is fixed on the spiral block 121 .
[0071] In this embodiment, when the Pasteur pipette 130 is inserted into the centrifuge tube 140 through the spiral block 121, the Pasteur pipette 130 is fixed on the spiral block 121 through the sealing airbag 131. Since the size of the sealing airbag 131 is larger than the size of the hole, the Pasteur pipette 130 is fixed on the spiral block 121.
[0072] The Pasteur pipette 130 can also be fixed on the spiral block 121 through the hole;
[0073] The fixed positions of the Pasteur pipette 130 and the spiral block 121 are not the center points, and can be rotated 360 degrees to select a suitable point for adsorption of the buffy coat layer, so that CGF rich in PRP and CD34+ stem cells can be collected quickly and accurately;
[0074] It should be noted that the connection between the Pasteur straw 130 and the sealing airbag 131 has a certain protrusion, the size of the protrusion is also larger than the size of the hole, and the protrusion and the Pasteur straw 130 are made of the same material. At the same time, the side of the spiral block 121 where the hole and the protrusion contact each other has a groove to facilitate the contact between the protrusion and the groove, so that the position of the Pasteur straw 130 and the spiral block 121 is fixed, and at the same time, the Pasteur straw 130 is fixed to the spiral block 121 through the sealing airbag 131, which causes the sealing airbag 131 to deform during use, thereby causing the suction end of the Pasteur straw 130 to shake.
[0075] The functions of each module in each device of the embodiment of the present utility model can refer to the corresponding description in the above method, which will not be repeated here.
[0076] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine different embodiments or examples described in this specification and the features of different embodiments or examples, unless they are contradictory.
[0077] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.
[0078] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can easily think of various changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.
Claims
1. A collection device for liquid concentrated growth factor CGF, characterized in that: include: A baffle, the baffle being detachably arranged on the centrifuge tube and having a through hole; A height adjustment component, the height adjustment component is disposed on the baffle through the through hole, and the height adjustment component moves relative to the baffle; A Pasteur pipette, one end of which passes through the height adjustment component and extends into the interior of the centrifuge tube. The depth of one end of the Pasteur pipette located inside the centrifuge tube is controlled by adjusting the position of the height adjustment component relative to the baffle.
2. A collection device for liquid concentrated growth factor CGF according to claim 1, characterized in that: The baffle is provided with an inner groove matched with the wall of the centrifuge tube, and the inner groove is connected with the through hole.
3. A collection device for liquid concentrated growth factor CGF according to claim 2, characterized in that: The baffle plate is provided with threads, the threads are located on the inner surface of the through hole, and the baffle plate is threadedly connected to the height adjustment assembly via the threads.
4. A collection device for liquid concentrated growth factor CGF according to claim 2, characterized in that: The height adjustment assembly comprises: A spiral block is threadedly connected to the baffle, and one end of the Pasteur pipette passes through the spiral block and extends into the centrifuge tube.
5. A collection device for liquid concentrated growth factor CGF according to claim 4, characterized in that: The height adjustment assembly also includes: A handle is provided on one end of the spiral block located outside the centrifuge tube.
6. The device for collecting liquid concentrated growth factor CGF according to claim 4, characterized in that: The Pasteur pipette has a sealing air bag on one end located outside the centrifuge tube.
7. The device for collecting liquid concentrated growth factor CGF according to claim 3, characterized in that: The diameter of the inner groove is greater than the diameter of the through hole.
8. The device for collecting liquid concentrated growth factor CGF according to claim 7, characterized in that: The size of the inner groove is matched with the size of the centrifuge tube orifice.
9. The device for collecting liquid concentrated growth factor CGF according to claim 6, characterized in that: The spiral block is provided with a hole for the Pasteur pipette to pass through, and the size of the hole is smaller than the size of the sealing airbag, so that the Pasteur pipette can be fixed on the spiral block.