Extraction device of earthworm active protein

Abstract

The earthworm active protein extraction device comprises a heating tank, an enzymolysis tank, a solid-liquid screener, an ultra-high-speed tubular centrifuge, a first buffer tank, a filter membrane assembly, a second buffer tank, a first concentration membrane assembly, a third buffer tank, a second concentration membrane assembly, a purification tank, a fourth buffer tank, a third concentration membrane assembly and a vacuum freeze dryer which are connected in sequence, according to the earthworm enzymolysis device disclosed by the utility model, the enzymolysis tank is used for carrying out enzymolysis on earthworms, so that compared with hydrolysis type enzymolysis, the treatment capacity of production process equipment can be reduced by 50%, the production time, the workload and the production cost are greatly reduced, water is saved, and the sewage treatment capacity is reduced; the tubular ceramic membrane is adopted, so that the concentrated solution is high in solid content and low in water content, and freeze drying is facilitated.

Description

Technical Field

[0001] The utility model belongs to the field of animal protein extraction, in particular to a device for extracting active earthworm protein, and in particular to a device for extracting active earthworm protein containing collagenase, plasmin and fibrinogenase activating factor. Background Art

[0002] Earthworms, also known as earthworms, are ancient annelids. Why can they survive even when broken into two segments? Research has revealed that a key reason is that they contain active proteins such as lumbrokinase, SOD, collagenase, plasmin, and plasminogen activator, which maintain or improve the worm's circulatory system. Furthermore, their skin contains collagen, which helps the worm maintain its normal crawling motion. Lumbrokinase, with a molecular weight of 16-45 kDa, is currently used in medical prescriptions as a treatment for cerebral thrombosis.

[0003] Extracted from earthworms, earthworm active proteins contain, in addition to lumbrokinase and SOD, collagenase, plasmin, and plasminogen activator, among other active proteins. With a molecular weight ranging from 5kD to 10kD, these short-chain molecules can enter the human microcirculatory system, reducing blood cell clumping and leukocyte adhesion, improving microcirculation, promoting smooth blood flow, and enhancing physiological functions. In 2009, the Ministry of Health's Document No. 18 designated earthworm proteins as a new resource food. Therefore, earthworm active proteins can be widely used in the healthcare industry as functional foods or regular foods. They can also be incorporated into lumbrokinase to enhance its synergistic therapeutic effects.

[0004] Patent CN2022200140331 discloses: a comprehensive earthworm extraction device, which comprises: a pulper (1), an enzymolysis tank (2), a butterfly centrifuge (3), a grease collection box (4), a high-speed tubular centrifuge (5), a clarification membrane assembly (6), an ultrafiltration concentration membrane assembly (7), a nanofiltration concentration membrane assembly (8), a first vacuum freeze dryer (9), a first concentration and desalination membrane assembly (10), a second vacuum freeze dryer (13), a second concentration and desalination membrane assembly (14), a third vacuum freezer (15), a solid residue collection box (16), a fourth vacuum freeze dryer (17), a sewage collection box (18) and a chromatography column (19), characterized in that: the pulper (1), the enzymolysis tank (2) and the butterfly centrifuge (3) are connected in sequence, the solid residue outlet of the butterfly centrifuge (3) is connected to the solid residue collection box (16), and the grease outlet of the butterfly centrifuge (3) is connected to the grease collection box (4). The clear liquid outlet of the butterfly centrifuge (3) is connected to the inlet of the high-speed tubular centrifuge (5), and the solid residue on the tube wall of the high-speed tubular centrifuge (5) is sent to the fourth vacuum freeze dryer (17) to obtain earthworm protein; first, the technology uses the butterfly centrifuge (3) to perform three-item separation of oil, water and residue. The core component of the disc centrifuge is the disc, which has a fast separation speed and is mainly used in industries with high-yield solid-liquid separation. It can also perform three-item separation of solid, liquid and oil, but the effect is extremely poor, and the mechanical parts such as the disc need to be disassembled for cleaning after each use, which has high time cost and labor cost, and is not suitable for application in small-scale biochemical extraction industries; second, the solid residue on the tube wall of the high-speed tubular centrifuge (5) is sent to the fourth vacuum freeze dryer (17) to obtain earthworm protein. As is well known, the solid residue on the tube wall of the high-speed tubular centrifuge (5) mainly consists of earthworm skin and impurities, which cannot be used as earthworm protein.

[0005] Patent CN2022100059724 discloses a method for comprehensive extraction of earthworms, characterized in that it includes the following steps: (1) slurrying: earthworms taken out of a cold storage are thawed at room temperature and then sent to a separate pulping machine for slurrying to obtain a slurry liquid, the particles of which are ≤100μm; (2) enzymatic hydrolysis: the slurry liquid is mixed with purified water in a ratio of 1:1 to obtain an earthworm mixture, and the earthworm mixture is sent to an enzymatic hydrolysis tank for enzymatic hydrolysis. Enzymatic hydrolysis is a process in which the earthworm mixture is dissolved at 45℃-55℃ by relying on the autolytic properties of the enzymes contained in it. First, pure water needs to be added in a ratio of 1:1 during enzymatic hydrolysis; second, the collagenase in the extract is inactivated at 45℃-55℃, because the inactivation temperature of collagenase is above 35℃. Old thrombi are formed by collagen, and without collagenase, the thrombi cannot be completely dissolved and removed. Summary of the Invention

[0006] The purpose of the present invention is to solve the defects of the above extraction devices and provide an extraction device for active protein of earthworms that can be widely used in the health industry.

[0007] In order to achieve the purpose of the invention, the utility model adopts the following technical solutions:

[0008] The utility model discloses an extraction device for active protein of earthworm, which comprises: an enzymolysis tank, a solid-liquid screener, an ultra-high-speed tubular centrifuge, a first buffer tank, a filtering membrane assembly, a second buffer tank, a first concentrating membrane assembly, a third buffer tank, a second concentrating membrane assembly, a pure water machine, an eluent tank, a leaching liquid tank, a solid residue and concentrated liquid tank, a third concentrating membrane assembly, a fourth buffer tank, a purification tank and a vacuum freeze dryer, a lumbrokinase concentrated liquid tank and an amino acid water-soluble fertilizer tank, wherein: the liquid outlet of the enzymolysis tank is connected to the liquid inlet of the solid-liquid screener in sequence through a one-way valve and a pump, the liquid outlet of the solid-liquid screener is connected to the liquid inlet of the ultra-high-speed tubular centrifuge in sequence through a one-way valve and a pump, and the liquid outlet of the ultra-high-speed tubular centrifuge is connected to the first The liquid inlet of the buffer tank is connected, and the slag discharged from the solid-liquid screener and the slag discharged from the ultra-high-speed tubular centrifuge are respectively sent to the solid slag and concentrated liquid tanks. The first buffer tank and the filter membrane assembly form a first circuit. A one-way valve is installed at the liquid return port of the first buffer tank, and a one-way valve and a pump are installed at the liquid outlet. A pressure gauge is installed on the filter membrane assembly. The clear liquid outlet of the filter membrane assembly is connected to the second buffer tank, and the concentrated liquid circulates in the above-mentioned first circuit. When the pressure of the first circuit reaches 0.6-0.7MPa, the concentrated liquid in the above-mentioned first circuit is connected to the solid slag and concentrated liquid tank through a one-way valve; the second buffer tank and the first concentrated membrane assembly form a second circuit, and a one-way valve is installed at the liquid return port of the second buffer tank, and a one-way valve and a pump are installed at the liquid outlet. A pressure gauge is installed on the first concentrating membrane assembly, the clear liquid outlet of the first concentrating membrane assembly is connected to the third buffer tank, the concentrated liquid circulates in the above-mentioned second loop, and when the pressure of the second loop reaches 0.6-0.7MPa, the concentrated liquid in the above-mentioned second loop is connected to the lumbrokinase concentrated liquid tank through a one-way valve; the third buffer tank and the second concentrating membrane assembly form a third loop, a one-way valve is installed at the return liquid port of the third buffer tank, and a one-way valve and a pump are installed at the liquid outlet, a pressure gauge is installed on the second concentrating membrane assembly, the clear liquid outlet of the second concentrating membrane assembly is connected to the amino acid water-soluble fertilizer tank, the concentrated liquid circulates in the above-mentioned third loop, and when the pressure of the third loop reaches 0.6-0.7MPa, the concentrated liquid in the above-mentioned third loop is connected to the amino acid water-soluble fertilizer tank through a one-way valve. The one-way valve and the pump are connected to the liquid inlet of the purification tank; the liquid inlet of the purification tank is also connected to the eluent tank and the rinse liquid tank respectively through a one-way valve, the liquid outlet of the purification tank is discharged through the one-way valve or connected to the liquid inlet of the fourth buffer tank through a pump, the fourth buffer tank and the third concentrating membrane assembly form a fourth loop, the liquid inlet of the fourth buffer tank is connected to the pure water machine through a one-way valve, the liquid outlet is sequentially equipped with a one-way valve and a pump, the third concentrating membrane assembly is equipped with a pressure gauge, the liquid at the clear liquid outlet of the third concentrating membrane assembly is discharged, the concentrated liquid circulates in the above-mentioned fourth loop, and when the pressure of the fourth loop reaches 0.6MPa several times, the concentrated liquid in the above-mentioned fourth loop is connected to the vacuum freeze dryer through a one-way valve to obtain the active protein of the earthworm.

[0009] The utility model discloses an extraction device for active protein of earthworms, which further comprises a heating tank, wherein the heating tank and the inner sleeve of the enzymolysis tank form a water circuit, and the hot water of the heating tank heats the enzymolysis tank.

[0010] The utility model discloses an extraction device for active protein of earthworms, which further comprises a platinum resistance thermometer and a controller. The platinum resistance thermometer is installed in an enzymolysis tank, and the controller is connected to the platinum resistance thermometer via an information line.

[0011] The utility model provides a device for extracting active protein from earthworms, wherein the clear liquid outlets of the filtering membrane assembly, the first concentrating membrane assembly, the second concentrating membrane assembly and the third concentrating membrane assembly are located at the upper parts of the above assemblies.

[0012] The utility model discloses an extraction device for active protein of earthworm, wherein: in the fourth loop, when the pressure gauge reaches 0.6MPa for the first time, a water purifier adds water to the fourth buffer tank through a one-way valve, and after repeating 3-5 times, when the pressure gauge reaches 0.6MPa again, the one-way valve leading to the vacuum freeze dryer is opened, and the active protein of earthworm is obtained through the vacuum freeze dryer.

[0013] The utility model provides a device for extracting active protein from earthworms, wherein the filtering membrane assembly, the first concentrating membrane assembly, the second concentrating membrane assembly and the third concentrating membrane assembly are all tubular ceramic membranes.

[0014] Compared with the prior art, the advantages of the device for extracting active protein from earthworms of the present invention are as follows:

[0015] 1) The membrane elements in the filtration membrane assembly and the concentration membrane assembly of the present invention adopt tubular ceramic membranes. Ceramic membranes are inorganic membranes. The pore size of the ceramic membranes is uniform and the molecular weight is relatively consistent, so that the molecular weight of the product is relatively consistent and the purity is high. Secondly, the ceramic membranes are not easily fouled and blocked, and the efficiency is high. Thirdly, the ceramic membranes have a long life and low cost. Fourthly, the ceramic membranes have high compressive strength and fast filtration speed. The concentrated liquid has a high solid content and a low water content, which is conducive to freeze drying.

[0016] 2) The utility model adopts an enzymatic hydrolysis tank to perform enzymatic hydrolysis on earthworms. Compared with hydrolysis, the processing capacity of production process equipment can be reduced by 50%, production time, workload and production cost are greatly reduced, and water is saved and sewage treatment volume is reduced.

[0017] 3) The utility model adopts an enzymatic hydrolysis tank, which uses the earthworm's own enzymes and enzyme aids to lyse the earthworm cells and release the protein. Since the protein does not undergo physical destruction and grinding in a pulping machine, the activity of the protein is higher. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the device for extracting active protein from earthworms according to the present invention.

[0019] exist Figure 1Among them, label 1 is a heating tank; label 2 is an enzymatic hydrolysis tank; label 3 is a controller; label 4 is a platinum resistance thermometer; label 5 is a solid-liquid screener; label 6 is an ultra-high-speed tubular centrifuge; label 7 is a first buffer tank; label 8 is a filtration membrane assembly; label 9 is a second buffer tank; label 10 is a first concentrating membrane assembly; label 11 is a third buffer tank; label 12 is a second concentrating membrane assembly; label 13 is a pure water machine; label 14 is an eluent tank; label 15 is a rinse tank; label 16 is a solid residue and concentrate tank; label 17 is a third concentrating membrane assembly; label 18 is a fourth buffer tank; label 19 is a purification tank; label 20 is a vacuum freeze dryer; label 21 is a one-way valve; label 22 is a pump; label 23 is a pressure gauge; label 24 is a lumbrokinase concentrate tank; label 25 is an amino acid water-soluble fertilizer tank; label 26 is an inner sleeve. DETAILED DESCRIPTION

[0020] like Figure 1As shown, the extraction device of earthworm active protein of the present invention includes: a heating tank 1, an enzymatic hydrolysis tank 2, a controller 3, a platinum resistance thermometer 4, a solid-liquid screener 5, an ultra-high-speed tubular centrifuge 6, a first buffer tank 7, a filter membrane assembly 8, a second buffer tank 9, a first concentrating membrane assembly 10, a third buffer tank 11, a second concentrating membrane assembly 12, a pure water machine 13, an eluent tank 14, a rinse tank 15, a solid residue and concentrated liquid tank 16, a third concentrated membrane assembly 17, a fourth buffer tank 18, a purification tank 19 and a vacuum freeze dryer 20, a lumbrokinase concentrate tank 24 and an amino acid water-soluble fertilizer tank 25.The heating tank 1 and the inner sleeve 26 of the enzymolysis tank 2 form a water circuit, the hot water of the heating tank 1 heats the enzymolysis tank 2, the platinum resistance thermometer 4 is installed in the enzymolysis tank 2, the controller 3 is connected to the platinum resistance thermometer 4 through an information line, the liquid outlet of the enzymolysis tank 2 is connected to the liquid inlet of the solid-liquid screener 5 through a one-way valve 21 and a pump 22 in turn, the liquid outlet of the solid-liquid screener 5 is connected to the liquid inlet of the ultra-high-speed tubular centrifuge 6 through a one-way valve 21 and a pump 22 in turn, the liquid outlet of the ultra-high-speed tubular centrifuge 6 is connected to the liquid inlet of the first buffer tank 7, the slag of the solid-liquid screener 5 and the slag of the ultra-high-speed tubular centrifuge 6 are respectively sent to the solid slag and concentrated liquid tank 16, the first buffer tank 7 and the filter membrane assembly 8 form a first circuit, in the first buffer tank 7 The return liquid port is equipped with a one-way valve 21, the liquid outlet is equipped with a one-way valve 21 and a pump 22, the filter membrane assembly 8 is equipped with a pressure gauge 23, the clear liquid outlet of the filter membrane assembly 8 is connected to the second buffer tank 9, and the concentrated liquid circulates in the above-mentioned first circuit. When the pressure of the first circuit reaches 0.6-0.7MPa, the concentrated liquid in the above-mentioned first circuit is connected to the solid residue and concentrated liquid tank 16 through the one-way valve 21; the second buffer tank 9 and the first concentrating membrane assembly 10 form a second circuit, the return liquid port of the second buffer tank 9 is equipped with a one-way valve 21, the liquid outlet is equipped with a one-way valve 21 and a pump 22, the first concentrating membrane assembly 10 is equipped with a pressure gauge 23, the clear liquid outlet of the first concentrating membrane assembly 10 is connected to the third buffer tank 11, and the concentrated liquid circulates in the above-mentioned second circuit. Circulation, when the pressure of the second circuit reaches 0.6MPa, the concentrate in the above-mentioned second circuit is connected to the lumbrokinase concentrate tank 24 through a one-way valve 21; the third buffer tank 11 and the second concentrating membrane assembly 12 form a third circuit, the return liquid port of the third buffer tank 11 is equipped with a one-way valve 21, the liquid outlet is equipped with a one-way valve 21 and a pump 22, the second concentrating membrane assembly 12 is equipped with a pressure gauge 23, the clear liquid outlet of the second concentrating membrane assembly 12 is connected to the amino acid water-soluble fertilizer tank 25, the concentrate circulates in the above-mentioned third circuit, when the pressure of the third circuit reaches 0.6MPa, the concentrate in the above-mentioned third circuit is connected to the liquid inlet of the purification tank 19 through the one-way valve 21 and the pump 22; the liquid inlet of the purification tank 19 is also respectively connected to the one-way valve 2 1 is connected to the eluent tank 14 and the eluent tank 15, the liquid outlet of the purification tank 19 is discharged through a one-way valve 21 or connected to the liquid inlet of the fourth buffer tank 18 through a pump 22, the fourth buffer tank 18 and the third concentrating and filtering membrane assembly 17 form a fourth loop, the liquid inlet of the fourth buffer tank 18 is connected to the water purifier 13 through a one-way valve 21, the liquid outlet is sequentially equipped with a one-way valve 21 and a pump 22, the third concentrating membrane assembly 17 is equipped with a pressure gauge 23, the liquid at the clear liquid outlet of the third concentrating membrane assembly 17 is discharged, and the concentrated liquid circulates in the above-mentioned fourth loop. When the pressure of the fourth loop reaches 0.6 MPa several times, the concentrated liquid in the above-mentioned fourth loop is connected to the vacuum freeze dryer 20 through the one-way valve 21 to obtain the active protein of the earthworm.

[0021] like Figure 1As shown, the clear liquid outlets of the filtering membrane assembly 8, the first concentrating membrane assembly 10, the second concentrating membrane assembly 12 and the third concentrating membrane assembly 17 are located at the upper part of the above-mentioned assemblies.

[0022] In the fourth circuit, when the pressure gauge 23 reaches 0.6-0.7 MPa for the first time, the water purifier 13 adds pure water of the same volume as the concentrated liquid to the fourth buffer tank 18 through the one-way valve 21. After repeating 3-5 times, when the pressure gauge 23 reaches 0.6 MPa again, the one-way valve 21 leading to the vacuum freeze dryer 20 is opened, and the active protein of the earthworm is obtained through the vacuum freeze dryer 20.

[0023] The solid-liquid screener 5 discharges residue at the top of the screener 5. The residue from the ultrahigh-speed tubular centrifuge 6 adheres to the drum wall of the ultrahigh-speed tubular centrifuge. To discharge the residue, shut down the centrifuge, open the centrifuge body, remove the drum, and scrape the residue off the drum wall with a special shovel. Rinse thoroughly with water, then reinstall the drum and restart operation.

[0024] The filtration membrane assembly 8, the first concentrating membrane assembly 10, the second concentrating membrane assembly 12 and the third concentrating membrane assembly 17 are all tubular ceramic membranes.

[0025] The present embodiment is achieved by the following method: the thawed earthworm and an enzymolysis aid such as white sugar are mixed and loaded into an enzymolysis tank 2, the weight ratio of the earthworm to the enzymolysis aid is 20:1, the earthworm is heated by the inner sleeve 26 of the inner wall of the heating tank 1 and the enzymolysis tank 2, and the temperature in the enzymolysis tank 2 is controlled to be 26-32°C by the controller 3. At this temperature, the earthworm's own enzyme can be used to lyse the autologous cells and release the protein, and the enzymolysis is carried out with the assistance of the enzymolysis aid. After the earthworm is completely enzymolyzed into an enzymolysis solution, the outlet one-way valve 21 below the enzymolysis tank 2 is opened, and the enzymolysis solution enters the solid-liquid screening machine 5 for solid-liquid separation under the action of the pump 22, and the earthworm skin is shaken. Under the action of , the solid residue and concentrated liquid tank 16 is sent from above the sieve plate in the solid-liquid screening machine 5. The enzymatic hydrolyzate passes through the sieve holes and peristaltic pump in the solid-liquid screening machine 5 and is sent from the inlet of the ultra-high-speed tubular centrifuge 6 to the ultra-high-speed tubular centrifuge 6 for solid-liquid separation. Under the action of the rotating force, the solid sticks to the drum wall in the ultra-high-speed tubular centrifuge 6. After cleaning, it is combined with the earthworm skin collected in the solid-liquid screening machine 5 and the concentrated liquid remaining in the first buffer tank 7, sent to the solid residue and concentrated liquid tank 16, and sent to the collagen process for collagen extraction; the clear liquid separated by the ultra-high-speed tubular centrifuge 6 flows out from the clear liquid outlet, enters the first buffer tank 7, passes through the pump 22 and the filter membrane assembly 8 inlet, The concentrated liquid is sent to the 60KD ceramic tubular membrane in the filter membrane assembly 8 for filtration. The concentrated liquid flowing out of the concentrated liquid outlet of the filter membrane assembly 8 passes through the one-way valve 21 and the pump 22, returns to the first buffer tank 7, mixes with the original solution in the first buffer tank 7, and enters the filter membrane assembly 8 again for repeated filtration. When the pressure of the pressure gauge reaches 0.6MPa, the filtration is stopped, and the concentrated liquid remaining in the first buffer tank 7 is combined with the solid residue removed in the ultra-high-speed tubular centrifuge 6 and the earthworm skin in the solid-liquid screening machine 5 and sent to the solid residue and concentrated liquid tank 16; the filtered clear liquid flows out through the clear liquid outlet of the filter membrane assembly 8, enters the second buffer tank 9, and is sent to the first concentrated liquid tank 9 through the pump 22. In the membrane assembly 10, the concentrated liquid flowing out of the concentrated liquid outlet of the first concentrating membrane assembly 10 returns to the second buffer tank 9 through a one-way valve, where it is mixed with the original liquid and continues to concentrate. When the pressure of the pressure gauge reaches 0.6MPa, the concentration is stopped, and the concentrated liquid in the second buffer tank 9 is sent to the lumbrokinase concentration tank 24, and then sent to the lumbrokinase production process to produce lumbrokinase; the clear liquid flowing out of the clear liquid outlet of the first concentrating membrane assembly 10 enters the second concentrating membrane assembly 12 through the third buffer tank 11 and the pump 22, and the clear liquid flowing out of the clear liquid outlet of the second concentrating membrane assembly 12 is sent to the amino acid water-soluble fertilizer tank 25 through a pipeline, and then sent to the amino acid water-soluble fertilizer process for the production of amino acid water-soluble fertilizer.When the pressure reaches 0.6MPa, concentration is stopped, and the concentrated solution in the third buffer tank 11 is adjusted to pH, and then sent to the purification tank 19 for purification, leaching and elution. The purification tank 19 is equipped with a DEAE-cellulose ion exchange purifier. The concentrated solution after pH adjustment and the DEAE-cellulose ion exchange purifier in the purification tank 19 are exchanged and purified, and the earthworm miscellaneous proteins that are not exchanged are washed out and discharged using the leaching liquid. The earthworm active protein that is exchanged is exchanged and eluted from the DEAE-cellulose ion exchange purifier using the eluent. The earthworm active protein eluate purified and eluted by the purification tank 19 enters the third concentration membrane assembly 17 through the fourth buffer tank 18 and the pump 22 for concentration. The third concentration membrane assembly The concentrate from the concentrate outlet of component 17 is returned to the fourth buffer tank 18 through a one-way valve. It is mixed with the eluate already in the fourth buffer tank 18 and then continues to enter the inlet of the third concentrating membrane assembly 17 for concentration. When the pressure on the pressure gauge reaches 0.6 MPa, the one-way valve 21 connecting the fourth buffer tank 18 and the water purifier 13 is opened. An amount of pure water equal to the amount of concentrate is intermittently added to the fourth buffer tank 18 to dissolve and dilute the salt in the concentrate, which is then removed through the membrane pores along with the clear liquid. This desalination process is repeated five times with pure water. When the pressure gauge pressure reaches 0.6 MPa after the fifth addition of pure water, the desalted concentrate in the fourth buffer tank 18 is fed through the one-way valve 21 and pump 22 to a vacuum freeze dryer 20 for vacuum freeze evaporation to produce dried earthworm active protein. The clear liquid from the clear liquid outlet of the third concentrating membrane assembly 17 and the eluate from the purification tank 19 are sent to a sewage treatment system for standard treatment.

[0026] The above-described embodiments are merely descriptions of preferred implementation methods of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made by ordinary technicians in this field to the technical solutions of the present invention should fall within the scope of protection determined by the claims of the present invention.

Claims

1. A device for extracting active protein from earthworms, comprising: An enzymolysis tank (2), a solid-liquid sifter (5), an ultra-high-speed tubular centrifuge (6), a first buffer tank (7), a filtration membrane assembly (8), a second buffer tank (9), a first concentration membrane assembly (10), a third buffer tank (11), a second concentration membrane assembly (12), a water purifier (13), an eluent tank (14), a rinse tank (15), a solid residue and concentrate tank (16), a third concentration membrane assembly (17), a fourth buffer tank (18), a purification tank (19), a vacuum freeze dryer (20), a lumbrokinase concentrate tank (24), and an amino acid water-soluble fertilizer tank (25), wherein the liquid outlet of the enzymolysis tank (2) is sequentially connected to a one-way valve (21) and a The pump (22) is connected to the liquid inlet of the solid-liquid screener (5), the liquid outlet of the solid-liquid screener (5) is connected to the liquid inlet of the ultra-high-speed tubular centrifuge (6) through the one-way valve (21) and the pump (22) in sequence, the liquid outlet of the ultra-high-speed tubular centrifuge (6) is connected to the liquid inlet of the first buffer tank (7), the slag discharged from the solid-liquid screener (5) and the slag discharged from the ultra-high-speed tubular centrifuge (6) are respectively sent to the solid slag and concentrated liquid tank (16), the first buffer tank (7) and the filter membrane assembly (8) form a first loop, the liquid return port of the first buffer tank (7) is equipped with a one-way valve (21), the liquid outlet is equipped with a one-way valve (21) and a pump (22), and the filter membrane assembly (8) is equipped with a pressure The first concentrating membrane assembly (10) is provided with a pressure gauge (23), the clear liquid outlet of the filtering membrane assembly (8) is connected to the second buffer tank (9), the concentrated liquid circulates in the first loop, and when the pressure of the first loop reaches 0.6-0.7 MPa, the concentrated liquid in the first loop is connected to the solid residue and concentrated liquid tank (16) through a one-way valve (21); the second buffer tank (9) and the first concentrating membrane assembly (10) form a second loop, the return liquid port of the second buffer tank (9) is equipped with a one-way valve (21), the liquid outlet is equipped with a one-way valve (21) and a pump (22), the first concentrating membrane assembly (10) is equipped with a pressure gauge (23), the clear liquid outlet of the first concentrating membrane assembly (10) is connected to the third buffer tank (11), and the concentrated liquid outlet is connected to the third buffer tank (11). The liquid circulates in the second loop. When the pressure in the second loop reaches 0.6-0.7 MPa, the concentrated liquid in the second loop is connected to the lumbrokinase concentrated liquid tank (24) through a one-way valve (21). The third buffer tank (11) and the second concentrated membrane assembly (12) form a third loop. The return liquid port of the third buffer tank (11) is equipped with a one-way valve (21), and the liquid outlet is equipped with a one-way valve (21) and a pump (22). The second concentrated membrane assembly (12) is equipped with a pressure gauge (23). The clear liquid outlet of the second concentrated membrane assembly (12) is connected to the amino acid water-soluble fertilizer tank (25). The concentrated liquid circulates in the third loop. When the pressure in the third loop reaches 0.6-0.7 MPa, the concentrated liquid in the second loop is connected to the amino acid water-soluble fertilizer tank (25).When the pressure is 7MPa, the concentrated liquid in the third loop is connected to the liquid inlet of the purification tank (19) through a one-way valve (21) and a pump (22); the liquid inlet of the purification tank (19) is also connected to the eluent tank (14) and the rinse liquid tank (15) through a one-way valve (21), respectively; the liquid outlet of the purification tank (19) is discharged through the one-way valve (21) or connected to the liquid inlet of the fourth buffer tank (18) through a pump (22); the fourth buffer tank (18) and the third concentration membrane assembly (17) form a fourth loop; in the fourth buffer The liquid inlet of the tank (18) is connected to the water purifier (13) through a one-way valve (21), and the liquid outlet is equipped with a one-way valve (21) and a pump (22) in sequence. The third concentration membrane assembly (17) is equipped with a pressure gauge (23). The liquid at the clear liquid outlet of the third concentration membrane assembly (17) is discharged, and the concentrated liquid circulates in the fourth loop. When the pressure of the fourth loop reaches 0.6 MPa several times, the concentrated liquid in the fourth loop is connected to a vacuum freeze dryer (20) through a one-way valve (21) to obtain the active protein of earthworms.

2. The device for extracting active protein from earthworms as claimed in claim 1, characterized in that: It also includes: a heating tank (1), the heating tank (1) and the inner sleeve (26) of the enzymolysis tank (2) form a water circuit, and the hot water in the heating tank (1) heats the enzymolysis tank (2).

3. The device for extracting active protein from earthworms as claimed in claim 2, characterized in that: It also includes: a platinum resistance thermometer (4) and a controller (3), wherein the platinum resistance thermometer (4) is installed in the enzymolysis tank (2), and the controller (3) is connected to the platinum resistance thermometer (4) through an information line.

4. The device for extracting active protein from earthworms as claimed in claim 3, characterized in that: The clear liquid outlets of the filtering membrane assembly (8), the first concentrating membrane assembly (10), the second concentrating membrane assembly (12) and the third concentrating membrane assembly (17) are located at the upper part of the above-mentioned assemblies.

5. The device for extracting active protein from earthworms as claimed in claim 4, characterized in that: In the fourth loop, when the pressure gauge (23) reaches 0.6-0.7MPa for the first time, the water purifier (13) adds water to the fourth buffer tank (18) through the one-way valve (21). After repeating 3-5 times, when the pressure gauge (23) reaches 0.6MPa again, the one-way valve (21) leading to the vacuum freeze dryer (20) is opened, and the active protein of earthworms is obtained through the vacuum freeze dryer (20).

6. The device for extracting active protein from earthworms as claimed in claim 5, characterized in that: The filtering membrane assembly (8), the first concentrating membrane assembly (10), the second concentrating membrane assembly (12) and the third concentrating membrane assembly (17) are all tubular ceramic membranes.

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