Disinfection device

Abstract

The disinfection device comprises a box body, a partition plate and an upper cover, the partition plate is detachably and vertically installed in the box body, the interior of the box body is divided into a disinfection cavity and a liquid discarding cavity through the partition plate, the disinfection cavity and the liquid discarding cavity are communicated through a channel formed in the partition plate, and a filter piece is installed on the channel; preferably, the channel is formed in the bottom of the partition plate. The box body is provided with a liquid discarding pipe communicated with the liquid discarding cavity; the upper cover detachably covers the opening end of the box body; through the improvement of the box body, the partition plate and the upper cover, the box can be detached, is simple and convenient to operate, and is convenient to clean, disinfect and reuse.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental devices, in particular to a disinfection device used for disinfecting the surface of plant tissues. Background Art

[0002] In the scientific research related to plant tissue culture, there is often a large area of ​​contamination in the tissue culture medium due to incomplete disinfection of the plant surface. On the one hand, such contamination will affect the growth of the plant, and on the other hand, it will cause certain interference with its subsequent scientific research. Although traditional chemical disinfection operations can kill a large number of microorganisms on the surface of plants, the process of adding samples and discarding liquid is cumbersome and slow, which can easily cause contamination. In addition, the current plant surface disinfection equipment is relatively large and inconvenient to operate.

[0003] After searching, it was found that the invention patent with publication number CN113498737A discloses an explant disinfection device, which relates to the field of plant tissue culture technology and includes a container, a partition device and a filter device. Among them, the first end of the container is provided with an opening. The partition device is arranged in the container to separate at least two chambers, one end of the partition device is arranged at the second end of the container, and the other end extends toward the opening, and each chamber is provided with an inlet for placing the explant at one end close to the opening. The filter device is detachably arranged in the container and is used to prevent the explants in each chamber from being lost. Various types of explants can be placed in different chambers for simultaneous disinfection to improve the processing efficiency. By providing a filter device, when pouring out the liquid in the container, the explants in the chamber can be prevented from being lost with the liquid, so as to achieve the effect of not wasting the explants. However, when pouring the disinfectant, the above-mentioned device is bulky and inconvenient to discharge. Utility Model Content

[0004] Therefore, in order to solve the above-mentioned shortcomings, the utility model provides a disinfection device suitable for disinfecting the surface of plants. Through the design of the box body, partition and upper cover, it can be detachable, easy to operate, and convenient for cleaning, disinfection and reuse.

[0005] Specifically, a plant tissue surface disinfection device includes a box body, a partition and an upper cover.

[0006] The partition is detachably installed vertically in the box body, and the partition divides the interior of the box body into a disinfection chamber and a waste liquid chamber. The disinfection chamber and the waste liquid chamber are connected through a channel opened in the partition, and a filter is installed on the channel; preferably, the channel is opened at the bottom of the partition;

[0007] The box body is provided with a liquid discarding tube communicated with the liquid discarding cavity;

[0008] The upper cover is detachably covered on the opening end of the box body.

[0009] Optionally, after the upper cover is closed on the box body, the inner bottom of the upper cover is tightly fitted with the partition, and a sample addition hole is opened on the upper cover. After the upper cover is closed on the box body, the sample addition hole is located above the disinfection chamber.

[0010] Optionally, the partition is installed by plugging into the box body, and a slot for installing the partition is provided on the inner wall and / or the bottom of the box body; preferably, the box body is provided with a positioning strip adapted to the slot; channels with filter strips are distributed on both sides of the positioning strip.

[0011] Optionally, the upper cover, the partition and the box body are all made of transparent material.

[0012] The utility model has the following advantages:

[0013] The utility model has the following beneficial effects:

[0014] The utility model provides a disinfection device for disinfecting the surface of plant tissues. The device can effectively disinfect the surface of plant tissues in combination with disinfection chemical reagents and a clean workbench, reducing microbial contamination of the culture medium of plant tissue culture. The device has a simple structure, is easy to operate, and is convenient for cleaning, disinfection, and reuse. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a three-dimensional schematic diagram of the utility model;

[0016] Figure 2 This is a schematic diagram of the utility model in a disassembled state;

[0017] Figure 3 This is a schematic diagram of the utility model after the upper cover is removed (the dotted line with an arrow indicates the flow direction of the disinfectant liquid)

[0018] Figure 4 is a three-dimensional schematic diagram of the box body;

[0019] Figure 5 is a three-dimensional schematic diagram of the partition;

[0020] In the figure: 100, box body; 101, disinfection chamber 101; 102, waste liquid chamber; 103, waste liquid pipe; 104, card slot;

[0021] 200, upper cover; 201, sample adding hole;

[0022] 300, partition; 301, positioning strip; 302, channel. DETAILED DESCRIPTION

[0023] Embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present application, and should not be construed as limiting the present application.

[0024] In this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises", or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article, or apparatus.

[0025] As described in the background technology, in the scientific research related to plant tissue culture, there is often a large area of ​​contamination in the tissue culture medium due to incomplete disinfection of the plant surface. On the one hand, such contamination will affect the growth of the plant, and on the other hand, it will cause certain interference to its subsequent scientific research. Although the traditional chemical disinfection operation can kill a large number of microorganisms on the surface of plants, it is easy to cause contamination because the process of adding samples and discarding liquid is cumbersome and slow, and the current plant surface disinfection device is relatively large and inconvenient to operate.

[0026] Based on the above reasons, this embodiment provides a disinfection device, which is suitable for disinfecting the surface of plant tissues, such as Figure 1-Figure 5 As shown, the disinfection device includes a box body 100, a partition 300 and an upper cover 200.

[0027] The partition 300 is detachably installed vertically in the box body 100, and the partition 300 divides the interior of the box body 100 into a disinfection chamber 101 and a waste liquid chamber 102. The disinfection chamber and the waste liquid chamber are connected through a channel 302 opened in the partition, and a filter is installed on the channel; preferably, the channel is opened at the bottom of the partition;

[0028] The box body is provided with a liquid discarding tube 103 communicating with the liquid discarding cavity;

[0029] The upper cover 200 is detachably covered on the opening end of the box body 100 .

[0030] After the upper cover 200 is covered on the box body 100, the inner bottom of the upper cover is tightly fitted with the partition (to achieve sealing at the contact point), and a sample addition hole 201 is opened on the upper cover 200. After the upper cover is covered on the box body, the sample addition hole 201 is located above the disinfection chamber 101 (such as Figure 3 As shown, the sample addition hole 201 is located above the disinfection chamber 101).

[0031] The above device can be used in conjunction with disinfecting chemical reagents and a clean workbench to effectively disinfect the surface of plant tissues and reduce microbial contamination of the culture medium for plant tissue culture. The device has a simple structure, is easy to operate, and is convenient for cleaning, disinfection, and reuse.

[0032] When the above technical features are used, the upper cover, the partition and the box body are first placed in a sterilizing pot at 121°C for sterilization for 20 minutes and then placed in an ultra-clean workbench for use.

[0033] When in use, the box body and the partition must be assembled together first, and then the plant tissue to be sterilized is placed on the other side of the waste liquid pipe after the box body partition is separated. Then, the upper cover is closed, the device is placed on the hand, and slightly tilted so that the waste liquid pipe side is higher than the sample side.

[0034] The operator adds sterile distilled water to one side of the partition through the sample hole, rinses the plant tissue slightly, and then tilts the device so that the side of the waste liquid tube is lower than the sample side, allowing the liquid to pass through the filter strip to the other side of the box body and then flow out through the waste liquid tube. This operation is repeated five times.

[0035] The operator adds 70% ethanol to one side of the partition through the sample hole. After the plant tissue is soaked for 30 seconds, the device is tilted so that the side of the liquid disposal tube is lower than the sample side, so that the liquid passes through the filter strip to the other side of the box body and then flows out through the liquid disposal tube. This operation is repeated twice.

[0036] The operator adds 4% sodium hypochlorite to one side of the partition through the sample hole. After the plant tissue is soaked for 8 minutes, the device is tilted so that one side of the waste liquid tube is lower than the sample side, allowing the liquid to pass through the filter strip to the other side of the box body and then flow out through the waste liquid tube.

[0037] The operator adds sterile distilled water to one side of the partition through the sample hole, rinses the plant tissue slightly, and then tilts the device so that the side of the waste liquid tube is lower than the sample side, allowing the liquid to pass through the filter strip to the other side of the box body and then flow out through the waste liquid tube. This operation is repeated three times.

[0038] Open the upper cover and take out the sterilized plant tissue for subsequent experiments.

[0039] In order to allow the disinfected liquid to flow better into the liquid disposal chamber, in one embodiment, the partition 300 is inserted and installed in the box body, and a slot 104 for installing the partition is opened on the inner wall and / or the bottom of the box body, and the slot can also position the partition; preferably, the box body is provided with a positioning strip 301 adapted to the slot; channels with filter strips are distributed on both sides of the positioning strip.

[0040] Exemplarily, the positioning strip is located at the bottom edge of the partition, and the lower edge of the partition has nine channels with filter strips, each three filter strips form a group, and there is a positioning strip between every two groups of filter strips.

[0041] In order to improve the sealing between the upper cover, the partition and the box body, in one embodiment, the height of the upper cover is lower than the box body, and when the upper cover and the box body are closed, the lower edge of the upper cover is slightly higher than the liquid disposal tube, the partition is slightly smaller than the box body, and the two sides of the partition can fit tightly with the two sides of the box body, the upper cover is slightly larger than the box body, and when the upper cover and the box body are closed, the upper cover and the liquid disposal tube are located on one side of the center line of the card slot.

[0042] In order to facilitate observation of the internal conditions (such as liquid flow) during disinfection, in one embodiment, the upper cover, the partition and the box body are all made of transparent material.

[0043] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A disinfection device, characterized in that: It includes a box body, a partition and an upper cover. The partition is detachably installed vertically in the box body, and the partition divides the interior of the box body into a disinfection chamber and a waste liquid chamber. The disinfection chamber and the waste liquid chamber are connected through a channel opened in the partition, and a filter is installed on the channel; The box body is provided with a liquid discarding tube communicated with the liquid discarding cavity; The upper cover is detachably covered on the opening end of the box body.

2. A disinfection device according to claim 1, characterized in that: The upper cover is provided with a sample adding hole, and the sample adding hole is located above the disinfection cavity.

3. A disinfection device according to claim 1, characterized in that: The channel is opened at the bottom of the partition.

4. A disinfection device according to claim 1, characterized in that: After the upper cover is closed on the box body, the inner bottom of the upper cover is tightly fitted with the partition.

5. A disinfection device according to claim 1, characterized in that: The partition is inserted and installed in the box body, and a slot for installing the partition is opened on the inner wall and / or the bottom of the box body.

6. A disinfection device according to claim 5, characterized in that: The box body is provided with a positioning strip adapted to the card slot.

7. A disinfection device according to claim 6, characterized in that: Channels with filter strips are distributed on both sides of the positioning strip.

8. A disinfection device according to any one of claims 1 to 7, characterized in that: The upper cover, the partition plate and the box body are all made of transparent material.

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