Slide glass waterproof device

Abstract

The utility model relates to a slide glass waterproof device, which comprises a cover plate, the cover plate has a preset thickness, the lower surface of the cover plate is recessed to form a first accommodating space, a first clamping part is arranged on one side of the first accommodating space, a first connecting part is arranged at one end, close to the first clamping part, of the cover plate, and a first waterproof part is arranged around the edge of the first accommodating space; the base has a preset thickness, the upper surface of the base is sunken to form a second accommodating space, a second clamping part is arranged on one side of the second accommodating space, a second connecting part is arranged at one end, close to the second clamping part, of the base, and a second waterproof part is arranged around the edge of the second accommodating space; wherein the first connecting part and the second connecting part are hinged together, so that the cover plate and the base can rotate in the axial direction of the first connecting part and the second connecting part, and after the cover plate and the base are closed, the first containing space and the second containing space are combined to form a waterproof space, so that the non-contact area of the slide glass is located in the waterproof space, and the contact area of the slide glass is located outside the waterproof space.

Description

Technical Field

[0001] The present application relates to the field of mechanical technology, specifically to biological experimental equipment, and in particular to a slide waterproof device. Background Art

[0002] In existing biological detection reagents, the slides used (such as fluorescent slides) can be of a multi-person design, that is, a slide contains multiple biological slices, and multiple samples can be detected simultaneously, thereby improving detection efficiency.

[0003] However, since the number of samples tested each time may vary, some bio-slices on some slides may not contain samples. During the slide experiment, it is necessary to go through processes such as cleaning and soaking. Bio-slices without samples cannot be used again after contacting water. Therefore, when multiple slides are used for testing each time, reagents may be wasted and the testing cost may increase. Utility Model Content

[0004] In view of the technical problems existing in the prior art, the present application proposes a wafer-carrying waterproof device, and specifically provides the following technical solutions:

[0005] A wafer carrier waterproof device, comprising: a cover plate, which has a predetermined thickness and a concave lower surface, forming a first accommodating space, a first clamping portion is provided on one side of the first accommodating space, a first connecting portion is provided at one end of the cover plate close to the first clamping portion, and a first waterproof portion is provided around the edge of the first accommodating space; and a base, which has a predetermined thickness and a concave upper surface, forming a second accommodating space, a second clamping portion is provided on one side of the second accommodating space, a second connecting portion is provided at one end of the base close to the second clamping portion, and a second waterproof portion is provided around the edge of the second accommodating space; wherein the first connecting portion and the second connecting portion are hinged together so that the cover plate and the base can rotate along the axial direction of the first connecting portion and the second connecting portion, and after the cover plate and the base are closed, the first accommodating space and the second accommodating space are combined to form a waterproof space; when in use, the wafer carrier is located between the cover plate and the base, and is sealed by the first waterproof portion and the second waterproof portion, so that the non-contact area of ​​the wafer carrier is in the waterproof space, and the contact area is outside the waterproof space.

[0006] Optionally, the height of the first waterproof portion is higher than the edge of the first accommodating space, and the height of the second waterproof portion is higher than the edge of the second accommodating space.

[0007] Optionally, the first clamping portion and the second clamping portion are both stepped structures, and the position of the carrier does not exceed the highest step of the first clamping portion and the second clamping portion when in use, and when one end of the carrier abuts against the first clamping portion or the second clamping portion, the first clamping portion and the second clamping portion further clamp the carrier after the cover and the base are closed.

[0008] Optionally, the bottom surfaces of the first accommodating space and the second accommodating space are transparent.

[0009] Optionally, the first waterproof part and the second waterproof part are both waterproof rubber strips or silica gel, which are arranged by bonding or interlocking.

[0010] Optionally, the slide comprises a fluorescent slide, and the slide comprises a plurality of test areas.

[0011] Optionally, a first waterproof portion prompt line is provided on the upper surface of the cover plate, and the first waterproof portion prompt line is located directly above the first waterproof portion.

[0012] Optionally, a second waterproof portion prompt line is provided on the lower surface of the base, and the second waterproof portion prompt line is located directly below the second waterproof portion.

[0013] Optionally, a buckle is provided on the cover plate and / or the base, and the buckle is used to lock the cover plate and the base.

[0014] The slide waterproof device of the present application can quickly seal the unused part of the slide, protect it from water and pollution during the experiment, and allow it to be used normally in the next experiment, effectively reducing the detection cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Below, the preferred embodiments of the present application will be further described in detail with reference to the accompanying drawings, wherein:

[0016] Figure 1 is a schematic structural diagram of a wafer-carrying waterproof device according to an embodiment of the present application;

[0017] Figure 2 is a schematic structural diagram of a wafer-carrying waterproof device from another angle according to an embodiment of the present application;

[0018] Figure 3 This is a schematic structural diagram of a wafer-carrying waterproof device in a closed state according to an embodiment of the present application;

[0019] Figure 4 is a schematic diagram of a wafer-carrying waterproof device in use according to an embodiment of the present application; and

[0020] Figure 5 This is a schematic diagram of a wafer-carrying waterproof device in a closed state when in use according to an embodiment of the present application. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0022] In the following detailed description, reference may be made to the various specification drawings that are part of the present application and are used to illustrate specific embodiments of the present application. In the accompanying drawings, similar reference numerals describe substantially similar components in different figures. The various specific embodiments of the present application are described below in sufficient detail so that a person of ordinary skill in the art with relevant knowledge and skills in the art can implement the technical solutions of the present application. It should be understood that other embodiments may also be used or structural, logical or electrical changes may be made to the embodiments of the present application.

[0023] In order to solve the problem of slide waste, the utility model proposes a slide waterproof device. The slide involved in the present application can be any transparent glass sheet used for biological experiments, used to carry samples in experiments. In particular, the slide involved can be a glass slide used for immunofluorescence (IFA) analysis and detection.

[0024] like Figure 1 and Figure 2 As shown, the wafer-carrying waterproof device comprises: a cover plate 11 and a base 21. The cover plate 11 and the base 21 are substantially rectangular. The cover plate 11 has an upper surface 101 and a lower surface 102. The base 21 has an upper surface 201 and a lower surface (not shown in the figure).

[0025] The cover plate 11 has a predetermined thickness and the lower surface 102 is concave, forming a first accommodating space 111. A first clamping portion 121 is provided on one side of the first accommodating space 111, a first connecting portion 131 is provided on one end of the cover plate 11 close to the first clamping portion 121, and a first waterproof portion 141 is provided around the edge of the first accommodating space 111. The base 21 has a predetermined thickness and the upper surface 201 is concave, forming a second accommodating space 211. A second clamping portion 221 is provided on one side of the second accommodating space 211, a second connecting portion 231 is provided on one end of the base 21 close to the second clamping portion 221, and a second waterproof portion 241 is provided around the edge of the second accommodating space 211. The first connecting portion 131 and the second connecting portion 231 are hinged together so that the cover plate 11 and the base 21 can rotate along the axial direction of the first connecting portion 131 and the second connecting portion 231.

[0026] After the cover 11 and the base 21 are closed, the first accommodating space 111 and the second accommodating space 211 are combined to form a waterproof space.

[0027] like Figure 3 In some embodiments, the cover plate 11 and / or the base 21 are provided with a buckle 251, and the buckle 251 is used to lock the cover plate 11 and the base 21.

[0028] like Figure 4 and Figure 5 As shown, when in use, the carrier film 31 is located between the cover plate 11 and the base 21 and is clamped by the cover plate 11 and the base 21, and is sealed by the first waterproof portion 141 and the second waterproof portion 241, so that the non-contact area of ​​the carrier film 31 is in the waterproof space and the contact area is outside the waterproof space.

[0029] In some embodiments, slide 31 comprises a fluorescent slide. In some embodiments, slide 31 comprises a plurality of test areas.

[0030] Taking an immunofluorescence test as an example, the fluorescent slide used for the test is generally designed for multiple people, and a slide 31 contains multiple biological slices (for example, 10). The specific experimental steps may include:

[0031] Sample incubation: According to the test plan, add 30 μL of the diluted sample to be tested to each reaction area of ​​the test slide in turn, and incubate at room temperature (18-25°C) for 30 minutes. Avoid drying of the liquid during the incubation process. If necessary, a wet box can be used for incubation.

[0032] First wash: Rinse the slides in a beaker of PBS Tween buffer, then immediately immerse them in a cup of PBS Tween buffer for at least 5 minutes. Replace the buffer after washing up to 16 slides each time.

[0033] Secondary antibody incubation: Take the slide out of the wash cup, gently drain the excess water and immediately wipe the back and edge water with absorbent paper, then immediately add 25 μL of FITC-labeled secondary antibody to each reaction area of ​​the slide, and incubate at room temperature (18-25°C) in the dark for 30 minutes. During the incubation process, avoid drying of the liquid. If necessary, a wet box can be used for incubation.

[0034] Second wash: Rinse the slides in a beaker of PBS Tween buffer, then immediately immerse them in a cup of PBS Tween buffer for at least 5 minutes. Replace the buffer after washing up to 16 slides each time.

[0035] Sealing: Take out the slide from the washing cup, gently shake off excess water and immediately wipe off the water on the back and edges with absorbent paper, place it on the slide holder, add a drop of sealing agent directly to each reaction area of ​​the slide with a dropper bottle (avoid too much or too little sealing agent), and gently cover the cover glass on the test slide, trying to avoid bubbles.

[0036] Result judgment: Fluorescence mode is interpreted by fluorescence microscope. Recommendation: Use 20× objective lens when observing tissues, organs, infected or transfected cells, and 40× objective lens when observing cell matrix. Excitation filter: 450-490nm, spectroscopic filter: 510nm, sun-blocking filter: 515nm.

[0037] From the above steps, it can be seen that during the experiment, the slides have to go through multiple washing and soaking processes. In this process, the biological slices without samples will also go through the washing and soaking process, which will inevitably cause the contamination and damage of these biological slices, resulting in unnecessary waste.

[0038] Therefore, the present application proposes a slide waterproof device, which can quickly seal the unused part of the slide, protect it from water and pollution during the experiment, so that it can be used normally in the next experiment, effectively reducing the detection cost.

[0039] In some embodiments, the height of the first waterproof portion 141 is higher than the edge of the first accommodating space 211, and the height of the second waterproof portion 241 is higher than the edge of the second accommodating space 211. In some embodiments, the first waterproof portion 141 and the second waterproof portion 241 are flexible to improve the waterproof effect and the stability of the clamping.

[0040] In some embodiments, the first clamping portion 121 and the second clamping portion 221 are both stepped structures. When in use, the position of the carrier film 31 does not exceed the highest step of the first clamping portion 121 and the second clamping portion 221. When one end of the carrier film 31 abuts against the first clamping portion 121 or the second clamping portion 221, the first clamping portion and the second clamping portion further clamp the carrier film after the cover plate and the base are closed. The stepped structure leaves space for the carrier film 31, allowing the carrier film 31 to be clamped between the two stepped structures to further improve the stability of the carrier film waterproof device.

[0041] In some embodiments, when using the wafer waterproof device, the wafer does not necessarily have to be clamped in the first clamping portion 121 or the second clamping portion 221 every time, depending on how much area of ​​the wafer 31 is used. For example, most of the area of ​​the wafer 31 is to be involved in the experiment, and only a small part needs to be isolated from the liquid. Then this small part can be placed in the waterproof space, and the wafer 31 does not rest against the first clamping portion 121 or the second clamping portion 221. In some embodiments, any part of the wafer 31 can be placed in the waterproof space, which does not affect the waterproof effect of the wafer waterproof device and the stability of the clamping.

[0042] In some embodiments, the bottom surfaces of the first accommodating space 111 and the second accommodating space 211 are transparent, so that it is convenient for experimenters to observe whether the inside thereof is affected.

[0043] In some embodiments, the first waterproof portion 141 and the second waterproof portion 241 are both waterproof rubber strips or silicone rubber, which are arranged by bonding or interlocking.

[0044] In some embodiments, a first waterproof portion reminder line (not shown in the figure) is provided on the upper surface 101 of the cover plate 11 , and the first waterproof portion reminder line is located directly above the first waterproof portion 141 .

[0045] In some embodiments, a second waterproof portion reminder line (not shown in the figure) is provided on the lower surface of the base 21 , and the second waterproof portion reminder line is located directly below the second waterproof portion 241 .

[0046] The first waterproof part prompt line and the second waterproof part prompt line are used to prompt the experimenter the position of the waterproof part, so that the experimenter will know the downward pressing position after the cover plate 11 and the base 21 are closed, which is convenient for the experimenter to accurately place the carrier 31.

[0047] The slide waterproof device of the present application can quickly seal the unused part of the slide, protect it from water and pollution during the experiment, and allow it to be used normally in the next experiment, effectively reducing the detection cost.

[0048] The above embodiments are only used to illustrate the present application, and are not intended to limit the present application. Ordinary technicians in the relevant technical field may make various changes and modifications without departing from the scope of the present application. Therefore, all equivalent technical solutions should also fall within the scope disclosed in the present application.

Claims

1. A wafer-carrying waterproof device, characterized in that: include: A cover plate having a predetermined thickness and a concave lower surface to form a first accommodating space, a first clamping portion being provided on one side of the first accommodating space, a first connecting portion being provided at one end of the cover plate close to the first clamping portion, and a first waterproof portion being provided around an edge of the first accommodating space; and A base having a predetermined thickness and a concave upper surface to form a second accommodating space, a second clamping portion is provided on one side of the second accommodating space, a second connecting portion is provided at one end of the base close to the second clamping portion, and a second waterproof portion is provided around the edge of the second accommodating space; Among them, the first connecting part and the second connecting part are hinged together so that the cover and the base can rotate along the axial direction of the first connecting part and the second connecting part. After the cover and the base are closed, the first accommodating space and the second accommodating space are combined to form a waterproof space; when in use, the carrier is located between the cover and the base, and is sealed by the first waterproof part and the second waterproof part, so that the non-contact area of ​​the carrier is in the waterproof space, and the contact area is outside the waterproof space.

2. The wafer-carrying waterproof device according to claim 1, characterized in that: The height of the first waterproof portion is higher than the edge of the first accommodating space, and the height of the second waterproof portion is higher than the edge of the second accommodating space.

3. The wafer-carrying waterproof device according to claim 1, characterized in that: The first clamping part and the second clamping part are both stepped structures. When in use, the position of the carrier does not exceed the highest step of the first clamping part and the second clamping part. When one end of the carrier abuts against the first clamping part or the second clamping part, the first clamping part and the second clamping part further clamp the carrier after the cover and the base are closed.

4. The wafer-carrying waterproof device according to claim 1, characterized in that: The bottom surfaces of the first accommodating space and the second accommodating space are transparent.

5. The wafer-carrying waterproof device according to claim 1, characterized in that: The first waterproof part and the second waterproof part are both waterproof rubber strips or silica gel, and are arranged by bonding or interlocking.

6. The wafer-carrying waterproof device according to claim 1, characterized in that: The slide comprises a fluorescent slide, and the slide comprises a plurality of test areas.

7. The wafer-carrying waterproof device according to claim 1, characterized in that: A first waterproof portion prompt line is disposed on the upper surface of the cover plate, and the first waterproof portion prompt line is located directly above the first waterproof portion.

8. The wafer-carrying waterproof device according to claim 1, characterized in that: A second waterproof portion prompt line is disposed on the lower surface of the base, and the second waterproof portion prompt line is located directly below the second waterproof portion.

9. The wafer-carrying waterproof device according to claim 1, characterized in that: The cover plate and / or the base are provided with buckles, and the buckles are used to lock the cover plate and the base.

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