Mouse intestinal secretion collecting device for CRD stimulation

Abstract

The utility model belongs to the field of medical experiment instruments, and particularly relates to a mouse intestinal secretion collecting device for CRD stimulation, which comprises a catheter. A guide wire is arranged in the catheter; a cap is arranged at the end part of the catheter; a sampling swab is fixedly connected to one end, close to the cap, of the guide wire; an inflation valve is fixedly connected to the end, close to the end away from the cap, of the catheter. According to the step, the catheter, the guide wire, the sampling swab, the inflation valve and the cuff are arranged, so that intestinal secretion of the mouse under CRD stimulation can be sampled and collected when the visceral pain threshold of the mouse is detected, a worker can conveniently explore the molecular mechanism of the visceral pain of the mouse, and the length scale marks are arranged in the middles of the catheter and the guide wire, so that the detection accuracy of the visceral pain threshold of the mouse is improved. According to the mouse intestinal secretion sampling device, a worker can observe the insertion depth of a catheter and a guide wire and the relative position of the guide wire and the catheter more conveniently, the worker can confirm the insertion depth of a sampling swab more conveniently, and the worker can sample and collect intestinal secretion of a mouse more conveniently.

Description

Technical Field

[0001] The utility model belongs to the field of medical experimental instruments, in particular to a mouse intestinal secretion collection device for CRD stimulation. Background Art

[0002] Mice have typical mammalian organs and systems, strong reproductive capacity, easy access to transgenic strains and immunodeficient mice, and are easy to raise, so they are widely used in animal experiments. The reduction of visceral pain threshold can be caused by a variety of stress reactions, and the molecular mechanism is still unclear. It is necessary to use mice, especially transgenic mice, to explore it. Therefore, it is extremely important to study the molecular mechanism of visceral pain sensitivity in mice.

[0003] In the prior art, colorectal distension is widely used in basic experiments for studying the mechanism of visceral pain as a behavioral detection method for visceral pain threshold. When measuring the visceral pain threshold, a homemade distension balloon is generally used. The balloon is gently inserted into the colorectum of the mouse, and the balloon catheter is fixed to the base of the mouse's tail with pressure-sensitive tape, and the tail is fixed to a flat plate with tape. The entire mouse is covered with a transparent resin box and fixed to the flat plate. The end of the balloon catheter is connected to a sphygmomanometer and a syringe. The syringe is used to give the mouse different distension pressures, and the sphygmomanometer is used to indicate readings of different pressures.

[0004] During long-term observation, it was found that the behavioral signs of visceral pain in mice were abdominal muscle contraction and arched back. During the detection of visceral pain behavior, it was found that after CRD expansion, the colorectum of mice secreted a large amount of intestinal fluid, which adhered to the surface of the expansion balloon and could not be collected for further testing and analysis.

[0005] Traditional CRD balloon dilatation devices can only detect visceral pain thresholds but cannot collect intestinal secretions stimulated by CRD, which further affects the exploration of the molecular mechanisms of visceral pain.

[0006] To this end, the utility model provides a mouse intestinal secretion collection device for CRD stimulation. Utility Model Content

[0007] In order to make up for the deficiencies of the prior art and solve at least one of the problems raised in the background technology, a mouse intestinal secretion collection device for CRD stimulation is proposed.

[0008] The technical solution adopted by the utility model to solve its technical problems is: the utility model is a mouse intestinal secretion collection device for CRD stimulation, comprising a catheter; a guide wire is arranged inside the catheter; a cap is arranged at the end of the catheter; a sampling swab is fixedly connected to the end of the guide wire close to the cap; an inflation valve is fixedly connected to the end of the catheter close to the end away from the cap; a cuff is fixedly connected to the end of the catheter close to the cap; the cuff is made of elastic material; a length scale mark is arranged in the middle of the catheter; a length scale mark is arranged in the middle of the guide wire; this step is achieved by setting There are catheters, guide wires, sampling swabs, inflation valves and cuffs, which can be used to sample and collect intestinal secretions of mice under CRD stimulation when testing the visceral pain threshold of mice, thereby facilitating the staff's exploration of the molecular mechanism of visceral pain in mice. By providing length scale marks in the middle of the catheter and the guide wire, the staff can more conveniently observe the insertion depth of the catheter and the guide wire, as well as the relative position of the guide wire and the catheter, making it more convenient for the staff to confirm the insertion depth of the sampling swab, and making it more convenient for the staff to sample and collect the intestinal secretions of mice.

[0009] Preferably, the guide wire is made of a flexible metal material; the guide wire slides inside the catheter; the sampling swab is located in the middle of the cuff and can freely enter and exit the catheter; in this step, the guide wire is made of a flexible metal material, and can bend freely and move inside the catheter when the staff samples and collects mouse intestinal secretions, making it convenient for the staff to sample mouse intestinal secretions.

[0010] Preferably, the front end of the catheter and the cap are provided with a spiral buckle mechanism; in this step, by providing the spiral buckle mechanism at the front end of the catheter and the cap, the end of the catheter can be more conveniently fastened and sealed after the staff completes sampling of the mouse intestinal secretions, thereby reducing the possibility of foreign matter entering the interior of the catheter and contaminating the sampling sample of the sampling swab, thereby improving the accuracy of the staff's sampling of the mouse intestinal secretions.

[0011] Preferably, the cuff is an ellipsoid or a cylinder with a smooth outer circumference; the diameter of the cuff is 4.5 mm to 5.5 mm; the inner diameter of the catheter is 3 mm to 4 mm; in this step, by making the cuff an ellipsoid or a cylinder with a smooth outer circumference, the end of the device can be more conveniently inserted into the mouse intestine, thereby making it more convenient for the staff to sample the mouse intestinal secretions and perform mouse visceral pain threshold detection.

[0012] Preferably, the sampling swab is cylindrical or ellipsoidal with a diameter of 4 mm to 4.5 mm and is made of polyester fiber, rayon or flocked nylon. In this step, by making the sampling swab of polyester fiber, rayon or flocked nylon, it is more convenient to adsorb mouse intestinal secretions, thereby making it more convenient for staff to sample mouse intestinal secretions.

[0013] Preferably, the guide wire has a diameter of 1 mm to 1.5 mm.

[0014] Preferably, the cross-section of the cap is semi-elliptical; the surface of the cap is smooth; the cross-section of the bottom of the cap is circular, and the diameter is consistent with the end of the catheter; in this step, by setting the cross-section of the bottom of the cap to be circular, and the diameter is consistent with the end of the catheter, after the staff completes sampling of mouse intestinal secretions, the sealing effect of the cap on the end of the catheter can be improved, and the situation of foreign matter entering the interior of the catheter and contaminating the sampling sample of the sampling swab can be further improved, thereby further improving the accuracy of the staff's sampling of mouse intestinal secretions.

[0015] The beneficial effects of the utility model are as follows:

[0016] 1. The intestinal secretion collection device for mouse under CRD stimulation described in the utility model is provided with a catheter, a guide wire, a sampling swab, an inflation valve and a cuff. When the visceral pain threshold of mice is detected, the intestinal secretions of mice under CRD stimulation can be sampled and collected, thereby facilitating the staff to explore the molecular mechanism of visceral pain in mice.

[0017] 2. By making the guide wire from a flexible metal material, the guide wire can be bent freely and move inside the catheter when the staff is sampling and collecting the intestinal secretions of the mouse, making it convenient for the staff to sample the intestinal secretions of the mouse. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The utility model will be further described below in conjunction with the accompanying drawings.

[0019] Figure 1 It is a stereogram in the utility model;

[0020] Figure 2 It is a structural schematic diagram of the utility model;

[0021] Legend:

[0022] 1. Catheter; 2. Guide wire; 3. Sampling swab; 4. Cap; 5. Inflation valve; 6. Cuff. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Specific examples are given below.

[0025] like Figure 1 to Figure 2 As shown, a mouse intestinal secretion collection device for CRD stimulation described in an embodiment of the utility model comprises a catheter 1; a guide wire 2 is arranged inside the catheter 1; a cap 4 is arranged at the end of the catheter 1; a sampling swab 3 is fixedly connected to the end of the guide wire 2 close to the cap 4; an inflation valve 5 is fixedly connected to the end of the catheter 1 close to the end away from the cap 4; a cuff 6 is fixedly connected to the end of the catheter 1 close to the cap 4; the cuff 6 is made of elastic material; a length scale mark is arranged in the middle of the catheter 1; a length scale mark is arranged in the middle of the guide wire 2; when working, the staff removes the cap 4 at the end of the catheter 1, and then inserts the catheter 1 and the cuff 6 in a relaxed state into the colorectum of the mouse to be sampled. At this time, the staff uses the inflation valve 5 to inflate the inside of the catheter 1. When the mouse appears When there are behavioral indications of visceral pain such as abdominal muscle contraction and arched back, the staff moves the guide wire 2 forward and backward to drive the sampling swab 3 to sample a large amount of intestinal fluid secreted by the mouse rectum. This step is provided with a catheter 1, a guide wire 2, a sampling swab 3, an inflation valve 5 and a cuff 6. When the visceral pain threshold of the mouse is detected, the intestinal secretions of the mouse under CRD stimulation can be sampled and collected, thereby facilitating the staff to explore the molecular mechanism of visceral pain in the mouse. By providing length scale marks in the middle of the catheter 1 and the guide wire 2, the staff can more conveniently observe the insertion depth of the catheter 1 and the guide wire 2, as well as the relative positions of the guide wire 2 and the catheter 1, so that the staff can more conveniently confirm the insertion depth of the sampling swab 3, and the staff can more conveniently sample and collect the intestinal secretions of the mouse.

[0026] Further, such as Figure 1 to Figure 2 As shown, the guide wire 2 is made of a flexible metal material; the guide wire 2 slides inside the catheter 1; the sampling swab 3 is located in the middle of the cuff 6 and can freely enter and exit the catheter 1; in this step, the guide wire 2 is made of a flexible metal material, and can bend freely and move inside the catheter 1 when the staff samples and collects the intestinal secretions of the mouse, so that the staff can sample the intestinal secretions of the mouse.

[0027] Further, such as Figure 1 to Figure 2 As shown, the front end of the catheter 1 and the cover cap 4 are provided with a spiral buckle mechanism; in this step, by providing a spiral buckle mechanism at the front end of the catheter 1 and the cover cap 4, the end of the catheter 1 can be more conveniently buckled and sealed after the staff completes sampling of the mouse intestinal secretions, thereby reducing the possibility of foreign matter entering the interior of the catheter 1 and contaminating the sampling sample of the sampling swab 3, thereby improving the accuracy of the staff in sampling the mouse intestinal secretions.

[0028] Further, such as Figure 1 to Figure 2As shown, the cuff 6 is an ellipsoid or a cylinder with a smooth outer circumference; the diameter of the cuff 6 is 4.5mm to 5.5mm; the inner diameter of the catheter 1 is 3mm to 4mm; in this step, by making the cuff 6 an ellipsoid or a cylinder with a smooth outer circumference, the end of the device can be more conveniently inserted into the mouse intestine, thereby making it more convenient for the staff to sample the mouse intestinal secretions and perform mouse visceral pain threshold detection.

[0029] Further, such as Figure 2 As shown, the sampling swab 3 is cylindrical or ellipsoidal, with a diameter of 4mm to 4.5mm, and is made of polyester fiber, rayon or flocked nylon. In this step, by making the sampling swab 3 of polyester fiber, rayon or flocked nylon, it is more convenient to adsorb the intestinal secretions of mice, thereby making it more convenient for staff to sample the intestinal secretions of mice.

[0030] Further, such as Figure 1 to Figure 2 As shown, the diameter of the guide wire 2 is 1 mm to 1.5 mm.

[0031] Further, such as Figure 1 to Figure 2 As shown, the cross-section of the cap 4 is semi-elliptical; the surface of the cap 4 is smooth; the cross-section of the bottom of the cap 4 is circular, and the diameter is consistent with the end of the catheter 1; in this step, by setting the cross-section of the bottom of the cap 4 to be circular, and the diameter is consistent with the end of the catheter 1, the sealing effect of the cap 4 on the end of the catheter 1 can be improved after the staff completes the sampling of the mouse intestinal secretions, and the foreign matter can be further reduced from entering the inside of the catheter 1 and contaminating the sampling sample of the sampling swab 3, thereby further improving the accuracy of the staff in sampling the mouse intestinal secretions.

[0032] Working principle: The staff removes the cap 4 at the end of the catheter 1, and then inserts the catheter 1 and the cuff 6 in a relaxed state into the colorectum of the mouse that needs to be sampled. At this time, the staff uses the inflation valve 5 to inflate the inside of the catheter 1. When the mouse shows behavioral signs of visceral pain such as abdominal muscle contraction and arched back, the staff moves the guide wire 2 back and forth to drive the sampling swab 3 to sample a large amount of intestinal fluid secreted by the mouse rectum. The swab after sampling can continue to be used for subsequent tests to analyze the molecular composition of intestinal fluid under visceral pain stimulation, providing a molecular basis for future experiments. Compared with traditional CRD balloons, the utility model can not only meet the visceral pain stimulation of giving precise pressure, but also collect the intestinal fluid secreted by the intestine after CRD stimulation for subsequent purification and analysis, expanding traditional behavioral experiments to the cellular molecular level.

[0033] The above shows and describes the basic principles, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A mouse intestinal secretion collection device for CRD stimulation, comprising a catheter (1); characterized in that: A guide wire (2) is provided inside the catheter (1); a cap (4) is provided at the end of the catheter (1); a sampling swab (3) is fixedly connected to one end of the guide wire (2) close to the cap (4); an inflation valve (5) is fixedly connected to the end of the catheter (1) close to the end away from the cap (4); a cuff (6) is fixedly connected to the end of the catheter (1) close to the cap (4); the cuff (6) is made of elastic material; a length scale mark is provided in the middle of the catheter (1); and a length scale mark is provided in the middle of the guide wire (2).

2. The mouse intestinal secretion collection device for CRD stimulation according to claim 1, characterized in that: The guide wire (2) is made of a flexible metal material; the guide wire (2) is slidably fitted inside the catheter (1); the sampling swab (3) is located in the middle of the cuff (6) and can freely enter and exit the catheter (1).

3. The mouse intestinal secretion collection device for CRD stimulation according to claim 1, characterized in that: The front end of the catheter (1) and the cover cap (4) are provided with a spiral buckle mechanism.

4. The mouse intestinal secretion collection device for CRD stimulation according to claim 1, characterized in that: The cuff (6) is an ellipsoid or a cylinder with a smooth outer circumference; the diameter of the cuff (6) is 4.5 mm to 5.5 mm; and the inner diameter of the catheter (1) is 3 mm to 4 mm.

5. The mouse intestinal secretion collection device for CRD stimulation according to claim 1, characterized in that: The sampling swab (3) is cylindrical or ellipsoidal, with a diameter of 4 mm to 4.5 mm, and is made of polyester fiber or rayon or flocked nylon.

6. The mouse intestinal secretion collection device for CRD stimulation according to claim 1, characterized in that: The guide wire (2) has a diameter of 1 mm to 1.5 mm.

7. The mouse intestinal secretion collection device for CRD stimulation according to claim 1, characterized in that: The cross section of the cap (4) is semi-elliptical; the surface of the cap (4) is smooth; the cross section of the bottom of the cap (4) is circular, and the diameter is consistent with the end of the catheter (1).

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