Separating device and cracking elution instrument

Abstract

The utility model belongs to the field of flow cytometry, and discloses a separation device and a cracking elution instrument, the separation device comprises a flow tube, a flow carrier and a magnetic frame, the flow tube is used for accommodating a sample to be detected and a magnetic separation assembly, and the magnetic separation assembly can identify and combine antigens in the sample to be detected; the magnetic separation assembly is combined with the antigen to form an antigen-antibody conjugate, and the antigen-antibody conjugate can be combined with the secondary antibody to form a compound; the flow type carrier frame is provided with a mounting groove for embedding the flow type pipe, the bottom of the flow type carrier frame is provided with a hollow structure, and the bottom of the flow type pipe can extend out of the hollow structure; a magnetic ring is arranged at the top of the magnetic frame, the bottom of the flow type pipe can be sleeved with the magnetic ring, the magnetic ring has magnetism, the magnetic ring can magnetically attract the magnetic separation assembly, the magnetic separation assembly is made to be attached to the annular side wall of the bottom of the flow type pipe, and the flow type cell pretreatment device comprises a separation device. Meanwhile, inaccurate detection results caused by compound loss are avoided.

Description

Technical Field

[0001] The utility model relates to the field of flow cytometry, in particular to a separation device and a lysis and elution instrument. Background Art

[0002] When using flow cytometry for cell multi-factor joint detection projects, sample pretreatment operations must be performed in advance. Nanosphere antibody reagents are used in the sample pretreatment process to allow the nanospheres to bind to antigens in the sample, and then the nanospheres are detected to identify and measure cell multi-factors. During the entire flow sample pretreatment process, nanospheres are currently mostly enriched by centrifugation, and then the waste liquid in the flow tube is manually aspirated and discarded. This process needs to be performed twice, which is time-consuming and inefficient. The pretreatment speed of flow cytometry multi-factor projects is very slow. Therefore, separation devices and lysis and elution instruments are urgently needed to solve the above problems. Utility Model Content

[0003] One purpose of the utility model is to:

[0004] On the one hand, a separation device is provided, wherein a sample to be tested is pre-treated by a magnetic separation component, and a magnetic frame magnetically attracts the magnetic separation component in a flow tube so that the magnetic separation component is attached to the annular side wall at the bottom of the flow tube, thereby facilitating separation of the complex and the waste liquid;

[0005] On the other hand, a lysis and elution instrument is provided, including the above-mentioned separation device, which is convenient for eluting impurities and excess reagents, speeding up the pre-treatment of flow cells, and avoiding the loss of magnetic beads leading to inaccurate detection results.

[0006] To achieve this purpose, the utility model adopts the following technical solutions:

[0007] In a first aspect, a separation device is provided, comprising:

[0008] Magnetic separation components;

[0009] A flow tube, the flow tube is used to contain the sample to be tested and the magnetic separation component, the magnetic separation component can recognize and bind to the antigen in the sample to be tested, the magnetic separation component binds to the antigen to form an antigen-antibody conjugate, and the antigen-antibody conjugate can bind to a secondary antibody connected to fluorescein to form a complex;

[0010] A flow carrier, wherein the flow carrier is provided with a mounting groove for the flow tube to be embedded, and the bottom of the flow carrier is provided with a hollow structure, and the bottom of the flow tube can extend out of the hollow structure;

[0011] A magnetic frame, wherein a magnetic ring is provided on the top of the magnetic frame, and the magnetic ring can be sleeved on the bottom of the flow tube. The magnetic ring is magnetic and can selectively magnetically attract the magnetic separation component so that the magnetic separation component is attached to the bottom annular side wall of the flow tube.

[0012] Preferably, the magnetic separation component comprises magnetic beads and antibodies, and the magnetic beads are magnetic.

[0013] Preferably, the separation device comprises a detection antibody reagent, which is contained in the flow tube and comprises the antibody.

[0014] Preferably, the magnetic beads include several different coded magnetic beads, the detection antibody reagent includes several different coded antibodies, and the coded magnetic beads can bind to the corresponding coded antibodies.

[0015] Preferably, the flow carrier is provided with a plurality of the mounting grooves, and the magnetic frame is correspondingly provided with a plurality of the magnetic rings, and the plurality of the mounting grooves correspond one-to-one to the plurality of the magnetic rings.

[0016] Preferably, a limit frame is fixedly provided on the top of the magnetic frame, the limit frame is provided with an anti-idiot groove and a limit groove for the magnetic ring to be embedded, the flow carrier can be inserted into the anti-idiot groove, and the bottom of the anti-idiot groove can abut against the bottom of the flow carrier.

[0017] Preferably, the magnetic frame is an electromagnet, the magnetic frame is electrically connected to an external power supply, the magnetic ring is made of magnetic conductive material, and when the magnetic frame is powered on, the magnetic frame and the magnetic ring are magnetic.

[0018] Preferably, the magnetic ring is a permanent magnet;

[0019] The magnetic frame is movably arranged in the vertical direction, and the magnetic frame can drive the magnetic ring to separate from or approach the flow tube in the vertical direction;

[0020] And / or the flow carrier is movably arranged in the vertical direction, and the flow carrier can drive the flow tube to separate from or approach the magnetic ring in the vertical direction.

[0021] Preferably, the separation device also includes a working main frame, a slide rail is provided at one end of the working main frame close to the magnetic frame, a slider is provided between the magnetic frame and the working main frame, the slider is fixedly connected to the magnetic frame and slides with the slide rail.

[0022] In the second aspect, a lysis and elution instrument is provided, including a waste liquid treatment device, a magnetic bead recovery device and a separation device. The waste liquid treatment device can extend into the flow tube and the waste liquid treatment device can suck out the waste liquid in the flow tube and dispose of it as waste, and the magnetic bead recovery device is used to recover the complex.

[0023] The beneficial effects of the utility model are:

[0024] On the one hand, the utility model provides a separation device, including a flow tube, a flow carrier and a magnetic frame. The flow tube is used to accommodate a sample to be tested and a magnetic separation component. The flow tube is embedded in the installation groove of the flow carrier. The bottom of the flow carrier is provided with a hollow structure, and the bottom of the flow tube can extend from the hollow structure. The top of the magnetic frame is provided with a magnetic ring, which can be sleeved on the bottom of the flow tube. The magnetic ring is magnetic and can magnetically attract the magnetic separation component so that the magnetic separation component is attached to the bottom annular side wall of the flow tube. In actual work, the magnetic separation component can identify the antigen in the sample to be tested and combine with the antigen in the sample to be tested in the flow tube to form an antigen-antibody conjugate. The antigen-antibody conjugate can combine with a secondary antibody connected to fluorescein to form a complex. The complex can be used for detection. The complex includes the magnetic separation component. When the magnetic ring magnetically attracts the magnetic separation component, the complex is attached to the bottom annular side wall of the flow tube to achieve separation of the complex and the waste liquid.

[0025] On the other hand, the utility model provides a lysis and elution instrument, including a separation device, a waste liquid treatment device and a magnetic bead recovery device. A quantitative magnetic separation component is added to the flow tube. The separation device adsorbs the complex on the annular side wall at the bottom of the flow tube to achieve separation of the complex and the waste liquid. The waste liquid treatment device extends into the flow tube to absorb and treat the waste liquid to purify the complex. The magnetic bead recovery device recovers the complex for detection. The complex is attached to the annular side wall at the bottom of the flow tube to facilitate the elution of impurities and excess reagents, thereby accelerating the flow cell pre-treatment speed and avoiding the loss of the complex leading to inaccurate detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The utility model is further described in detail below according to the accompanying drawings and embodiments;

[0027] Figure 1 This is an axonometric view of the separation device of Example 1;

[0028] Figure 2 It is a partial exploded view of the separation device of Example 1;

[0029] Figure 3 This is an axonometric diagram of the lysis and elution apparatus of Example 2.

[0030] In the figure:

[0031] 1. Flow tube;

[0032] 2. Flow carrier; 21. Mounting slot; 22. Hollow structure; 23. Bearing layer; 24. Hollow layer;

[0033] 3. Magnetic frame; 31. Magnetic ring; 32. Limiting frame; 321. Limiting groove; 322. Anti-fool groove; 33. Electromagnetic coil; 34. Mounting plate;

[0034] 4. Working main frame; 41. Slide rail;

[0035] 5. Slider. DETAILED DESCRIPTION

[0036] In order to make the technical problems solved by the utility model, the technical solutions adopted and the technical effects achieved clearer, the technical solutions of the embodiments of the utility model will be further described in detail below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the utility model.

[0037] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0038] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0039] In the description of this article, it should be understood that the terms "upper", "lower", "left", "right", etc., and the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of description and simplified operation, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first" and "second" are only used to distinguish in the description and have no special meaning.

[0040] In the description of this specification, the description with reference to the terms "an embodiment", "example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example.

[0041] The technical solution of the utility model is further explained below with reference to the accompanying drawings and through specific implementation methods.

[0042] Embodiment 1:

[0043] like Figure 1 to Figure 2 As shown, this embodiment provides a lysis and elution instrument, including a waste liquid treatment device, a magnetic bead recovery device and a separation device, the separation device includes a flow tube 1, a flow carrier 2 and a magnetic frame 3, the flow tube 1 is used to accommodate a sample to be tested and a magnetic separation component, the flow tube 1 is embedded in a mounting groove 21 of the flow carrier 2, the bottom of the flow carrier 2 is provided with a hollow structure 22, the bottom of the flow tube 1 can extend from the hollow structure 22, the top of the magnetic frame 3 is provided with a magnetic ring 31, the magnetic ring 31 can be sleeved on the bottom of the flow tube 1, the magnetic ring 31 is magnetic, and the magnetic ring 31 can magnetically attract the magnetic separation component so that the magnetic separation component is attached to the bottom annular side wall of the flow tube 1. The magnetic separation component can identify the antigen in the sample to be tested and bind to the antigen in the sample to be tested in the flow tube 1 to form an antigen-antibody conjugate. The antigen-antibody conjugate can bind to the secondary antibody connected to the fluorescein to form a complex. The complex can be used for detection. The complex includes a magnetic separation component. When the magnetic ring 31 magnetically attracts the magnetic separation component, the complex adheres to the bottom annular side wall of the flow tube 1 to achieve separation of the complex from the waste liquid. The waste liquid treatment device can extend into the flow tube 1 and the waste liquid treatment device can suck out the waste liquid in the flow tube 1 and dispose of it. Since the complex is attached to the annular side wall of the flow tube 1, the waste liquid treatment device will not take away the complex when it extends into the bottom of the flow tube 1 to suck away the waste liquid, thereby avoiding the loss of the complex. The magnetic bead recovery device is used to recover the complex for detection.

[0044] In this embodiment, the magnetic separation component includes magnetic beads and antibodies. The magnetic beads have magnetism and can be combined with the antibodies to form the magnetic separation component. Further, the flow tube 1 is also used to contain a detection antibody reagent, and the detection antibody reagent includes antibodies.

[0045] In the actual operation process, a certain amount of magnetic beads, detection antibody reagents and samples to be tested are added to the flow tube 1. The magnetic beads first combine with the antibodies in the detection antibody reagent to form a magnetic separation component. The antibodies have the ability to recognize antigens. The magnetic separation component can recognize and bind to the antigens in the sample to be tested. The magnetic separation component combines with the antigen to form an antigen-antibody conjugate, and the antigen-antibody conjugate can be combined with the secondary antibody connected to the fluorescein to form a complex. After the complex is generated, the magnetic complex is magnetically attracted by the magnetic ring 31, so that the complex is attached to the bottom annular side wall of the flow tube 1 and separated from the waste liquid in the flow tube 1. At this time, the waste liquid treatment device extends into the flow tube 1 and absorbs the waste liquid to complete the purification of the complex. On the one hand, since the complex is attracted by the magnetic ring 31, the complex will not be sucked out, which is convenient for eluting impurities and excess reagents, and speeding up the pre-processing speed of the flow cell sample; on the other hand, the magnetic beads are quantitatively delivered according to the detection requirements, and the magnetic attraction of the complex by the magnetic ring 31 can effectively avoid the loss of the complex and cause inaccurate detection results.

[0046] Exemplarily, the magnetic ring 31 is set to an annular structure, the inner diameter of the magnetic ring 31 is equal to the outer diameter of the flow tube 1, and the bottom of the flow tube 1 can be inserted into the magnetic ring 31 to ensure that the composite is attached to the bottom annular side wall of the flow tube 1.

[0047] Exemplarily, the magnetic beads include several different encoded magnetic beads, the detection antibody reagents include several different encoded antibodies, the encoded magnetic beads can bind to the corresponding encoded antibodies, and different encoded antibodies can recognize different antigens. When the sample to be tested contains multiple antigens that need to be detected, multiple corresponding encoded magnetic beads and detection antibody reagents including multiple corresponding encoded antibodies are added to meet the multi-throughput detection requirements.

[0048] Exemplarily, the flow carrier 2 is provided with a plurality of mounting grooves 21, the magnetic frame 3 includes a plurality of magnetic rings 31, the plurality of mounting grooves correspond to the plurality of magnetic rings one by one, the magnetic frame 3 also includes a limit frame 32, the limit frame 32 is provided with a limit groove 321 and an anti-fool groove 322 for the magnetic ring 31 to be embedded, the flow carrier 2 can be inserted into the anti-fool groove 322, and the bottom of the anti-fool groove 322 can abut against the bottom of the flow carrier 2. The plurality of mounting grooves 21 can carry a plurality of flow tubes 1 at the same time, thereby improving the working efficiency of the separation device, the magnetic ring 31 is limited in the limit groove 321, and the flow carrier 2 can be inserted into the limit frame 32 along the anti-fool groove 322, thereby ensuring that the flow tube 1 can be inserted into the corresponding magnetic ring 31, thereby improving the operating accuracy and working efficiency.

[0049] Exemplarily, the flow carrier 2 includes a bearing layer 23 and a hollow layer 24, which are connected and supported by a bracket. A mounting groove 21 is provided in the bearing layer 23, and a hollow structure 22 is provided in the hollow layer 24. The diameter of the hollow structure 22 is smaller than the outer diameter of the flow tube 1. The hollow structure 22 can support the bottom of the flow tube 1 to prevent the flow tube 1 from falling. At least part of the bottom of the flow tube 1 passes through the hollow structure 22. The inner walls of the mounting groove 21 and the hollow structure 22 simultaneously limit the flow tube 1 to prevent the flow tube 1 from tilting radially. The bearing layer 23, the hollow layer 24 and the bracket are integrally arranged, or the bearing layer 23, the hollow layer 24 and the bracket are fixedly connected by welding or bonding, etc., which is simple to process and consumes less materials, thereby reducing the dead weight and production cost of the flow carrier 2.

[0050] Exemplarily, the magnetic frame 3 includes an electromagnetic coil 33, which is fixedly arranged at the bottom of the flow carrier 2, and the electromagnetic coil 33 is electrically connected to an external power supply. The magnetic ring 31 is made of a magnetic conductive material, and the magnetic ring 31 is fixedly connected to the electromagnetic coil 33. When the electromagnetic coil 33 is energized, the electromagnetic coil 33 and the magnetic ring 31 have magnetism. When the magnetic beads react and combine in the flow tube 1, the electromagnetic coil 33 is powered off, and the magnetic beads can float freely in the flow tube 1 and perform a binding reaction; when the complex is combined, the electromagnetic coil 33 is energized, and the electromagnetic coil 33 and the magnetic ring 31 are both magnetic, and can magnetically attract the magnetic beads so that the magnetic beads are attached to the bottom annular side wall of the flow tube 1. At this time, the waste liquid treatment device absorbs the waste liquid to purify the complex; when the waste liquid treatment is completed, the electromagnetic coil 33 is powered off, the electromagnetic coil 33 and the magnetic ring 31 lose their magnetism, and the magnetic bead recovery device can recover the complex.

[0051] Embodiment 2:

[0052] like Figure 3 As shown, based on the first embodiment, this embodiment provides another separation device, which is different from the first embodiment in that:

[0053] The magnetic ring 31 is a permanent magnet. The magnetic frame 3 includes a mounting plate 34 movably arranged in the vertical direction. The magnetic ring 31 is connected to the mounting plate 34 . The mounting plate can drive the magnetic ring 31 to separate from or approach the flow tube 1 in the vertical direction.

[0054] Exemplarily, the flow separation device also includes a working main frame 4, a slide rail 41 is provided at one end of the working main frame 4 close to the mounting plate 34, a slider 5 is provided between the mounting plate 34 and the working main frame 4, the slider 5 is fixedly connected to the mounting plate 34 and the slider 5 is slidably connected to the slide rail 41.

[0055] Optionally, the slide rail 41 is an electric slide rail.

[0056] In the actual working process, the mounting plate is first located at one end away from the flow carrier 2, and magnetic beads, test samples and detection antibody reagents are added to the flow tube 1. The magnetic beads gradually react and combine in the flow tube 1 to form a complex. After the magnetic beads react and combine, the electric slide drives the mounting plate 34 to approach the flow tube 1 until the magnetic ring 31 is sleeved on the bottom side wall of the flow tube 1. The magnetic ring 31 magnetically attracts the complex so that the complex adheres to the bottom annular side wall of the flow tube 1. The waste liquid treatment device absorbs the waste liquid to purify the complex. After the purification treatment is completed, the electric slide drives the mounting plate 34 away from the flow tube 1 without affecting the magnetic bead recovery device to recover the complex.

[0057] In other embodiments, the flow carrier 2 is movably disposed in the vertical direction, and the flow carrier 2 can drive the flow tube 1 to separate from or approach the magnetic ring 31 in the vertical direction.

[0058] In other embodiments, the magnetic frame 3 includes an electromagnetic coil 33 and a mounting plate 34, and the staff selects the separation method of the magnetic separation component according to actual needs.

[0059] In addition, the above are only preferred embodiments of the present invention and the technical principles used. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and that various obvious changes, readjustments and substitutions can be made by those skilled in the art without departing from the scope of protection of the present invention. Therefore, although the present invention is described in more detail through the above embodiments, the present invention is not limited to the above embodiments, and may include more other equivalent embodiments without departing from the concept of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. A separation device, characterized in that: The separation device comprises: Magnetic separation components; A flow tube (1), the flow tube (1) is used to contain a sample to be tested and the magnetic separation component, the magnetic separation component can recognize and bind to an antigen in the sample to be tested, the magnetic separation component binds to the antigen to form an antigen-antibody conjugate, and the antigen-antibody conjugate can bind to a secondary antibody connected to a fluorescein to form a complex; A flow carrier (2), the flow carrier (2) being provided with a mounting groove (21) for the flow tube (1) to be embedded, the bottom of the flow carrier (2) being provided with a hollow structure (22), and the bottom of the flow tube (1) being able to extend out of the hollow structure (22); A magnetic frame (3), the magnetic frame (3) comprising a magnetic ring (31), the magnetic ring (31) being able to be sleeved on the bottom of the flow tube (1), the magnetic ring (31) being magnetic, and the magnetic ring (31) being able to selectively magnetically attract the magnetic separation component so that the magnetic separation component is attached to the bottom annular side wall of the flow tube (1).

2. The separation device according to claim 1, characterized in that: The magnetic separation component comprises magnetic beads and antibodies, and the magnetic beads are magnetic.

3. The separation device according to claim 2, characterized in that: The separation device comprises a detection antibody reagent, which is contained in the flow tube (1) and comprises the antibody.

4. The separation device according to claim 3, characterized in that: The magnetic beads include several different coded magnetic beads, the detection antibody reagent includes several different coded antibodies, and the coded magnetic beads can bind to the corresponding coded antibodies.

5. The separation device according to claim 1, characterized in that: The flow carrier (2) is provided with a plurality of the mounting grooves (21), the magnetic frame (3) comprises a plurality of the magnetic rings (31), and the plurality of the mounting grooves (21) correspond one to one to the plurality of the magnetic rings (31).

6. The separation device according to claim 5, characterized in that A limit frame (32) is fixedly provided on the top of the magnetic frame (3), and the limit frame (32) is provided with an anti-mistake groove (322) and a limit groove (321) for the magnetic ring (31) to be embedded, and the flow carrier (2) can be inserted into the anti-mistake groove (322), and the bottom of the anti-mistake groove (322) can abut against the bottom of the flow carrier (2).

7. The separation device according to claim 1, characterized in that: The magnetic frame (3) comprises an electromagnetic coil (33), the electromagnetic coil (33) is electrically connected to an external power supply, and the magnetic ring (31) is made of a magnetic conductive material. When the electromagnetic coil (33) is energized, the electromagnetic coil (33) and the magnetic ring (31) are magnetic.

8. The separation device according to claim 1, characterized in that: The magnetic ring (31) is a permanent magnet; The magnetic frame (3) comprises a mounting plate (34) movably arranged in a vertical direction, the magnetic ring (31) is connected to the mounting plate (34), and the mounting plate (34) can drive the magnetic ring (31) to separate from or approach the flow tube (1) in a vertical direction; and / or The flow carrier (2) is movably arranged in a vertical direction, and the flow carrier (2) can drive the flow tube (1) to separate from or approach the magnetic ring (31) in the vertical direction.

9. The separation device according to claim 8, characterized in that The separation device further comprises a working main frame (4), a slide rail (41) is provided at one end of the working main frame (4) close to the magnetic frame (3), a slider (5) is provided between the magnetic frame (3) and the working main frame (4), the slider (5) is fixedly connected to the magnetic frame (3) and the slider (5) slides with the slide rail (41).

10. A lysis and elution apparatus, characterized in that: The lysis and elution instrument includes a waste liquid treatment device, a magnetic bead recovery device and a separation device as described in any one of claims 1 to 9, wherein the waste liquid treatment device can extend into the flow tube (1) and the waste liquid treatment device can suck out the waste liquid in the flow tube (1) and dispose of it as waste, and the magnetic bead recovery device is used to recover the complex.

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