In-vitro digestion device for predicting rice glycemic index

Abstract

The utility model relates to an in-vitro digestion device for predicting the glycemic index of rice, and relates to the field of in-vitro digestion experiments. The device is provided with a water bath kettle, a positioning frame mounted in the water bath kettle, a magnetic stirrer fixed at the bottom of the positioning frame, a reaction bottle placed on the positioning frame and corresponding to a stirring point of the magnetic stirrer, a pipetting mechanism, a disposable gun head module, a waste gun head box, a centrifugal tube module, an ethanol pool, an enzyme liquid pool and an electric control cabinet. Wherein the pipetting mechanism comprises a mechanical arm mounted on the workbench, a pipetting gun chuck mounted on the mechanical arm, and a plurality of groups of pipetting guns mounted on the pipetting gun chuck; a filter screen is arranged in the reaction bottle. Under the condition, high-throughput, high-precision and high-sensitivity rapid detection of the sample in the experiment process for predicting the rice glycemic index based on an in-vitro static digestion method is realized, and the experiment cost is reduced.

Description

Technical Field

[0001] The present application relates to the technical field of rice in vitro digestion experiments, and in particular to an in vitro digestion device for predicting the glycemic index of rice. Background Art

[0002] Carbohydrates can be absorbed through the metabolism of the human digestive system and enter the blood sugar in the form of glucose. They are the most important energy source for the human body, but they are also the most important reason for the increase in blood sugar levels after meals. Rice (Oryza sativa L.), as the most important source of carbohydrates in the human diet, is one of the staple foods for more than half of the world's population. Among them, starch is the main carbohydrate in rice, accounting for about 75%-80%, and its digestion behavior is the key to affecting blood sugar response. With the improvement of living standards, people are paying more and more attention to health, and some rice with medium and low glycemic index (GI) has also emerged with the continuous development of agronomy and breeding.

[0003] At present, the methods commonly used at home and abroad to study and determine the digestion process of rice in the human body mainly include in vivo models and in vitro models. Among them, in vivo experiments include human and animal experiments, but in vivo methods have problems such as being very expensive, time-consuming, inefficient, ethically controversial, unable to achieve large-scale detection of rice GI values, and large consumption of experimental samples. Therefore, a large number of researchers choose in vitro simulated digestion methods to predict and compare the GI value of rice.

[0004] In vitro models include dynamic digestion models and static digestion models. Among them, although a series of dynamic digestion devices that simulate the different stages of the human mouth, stomach, and intestines have been developed for in vitro dynamic digestion models, these devices are relatively complex and have high maintenance costs, and the repeatability and accuracy of sample testing are not high due to the relatively complex simulated environment.

[0005] In comparison, the in vitro static digestion model has the advantages of being simpler, more convenient, more repeatable, and less expensive. The existing in vitro static digestion method of rice is mainly completed by manual operation during the experiment, which leads to many problems, especially when simulating the human gastrointestinal digestion stage for manual sampling. Since it is necessary to ensure that the digestive enzyme solution and sampling operations are performed at different time points of digestion, manual operation is more prone to errors (especially when there are many samples to be tested), and the accuracy is difficult to guarantee during the addition and sampling process, and the experimental error increases. At the same time, due to the limitations of labor costs and operations, the scale of in vitro digestion experiments is limited, and it is difficult to achieve high-throughput and high-sensitivity rapid detection of samples. Utility Model Content

[0006] The purpose of the present application is to provide an in vitro digestion device for predicting the glycemic index of rice, so as to solve the technical problems of difficulty in achieving high-throughput, high-precision, and high-sensitivity rapid detection of samples and high experimental costs in the experimental process of predicting the glycemic index of rice based on the in vitro static digestion method.

[0007] To achieve the above purpose, the technical solution adopted in this application is:

[0008] An in vitro digestion device for predicting the glycemic index of rice, characterized in that it comprises: a workbench, a water bath installed on the workbench, a positioning frame installed in the water bath, a magnetic stirrer fixed to the bottom of the positioning frame, a reaction bottle placed on the positioning frame and corresponding to the stirring point of the magnetic stirrer, a pipetting mechanism installed on the workbench and located on one side of the water bath, a stand installed on the workbench and located on the other side of the water bath, a disposable gun head module installed on the stand, and a A discarded gun tip box, a centrifuge tube module installed on the stand, an ethanol pool installed on the stand, an enzyme solution pool installed on the stand, and an electric control cabinet installed on the workbench and located on one side of the stand; the pipetting mechanism includes a robotic arm installed on the workbench, a pipette gun chuck installed on the robotic arm, and a plurality of groups of pipette guns installed on the pipette gun chuck; a filter is provided in the reaction bottle; wherein the reaction bottle is used to hold a solution of rice grains after cooking, the ethanol pool is used to hold an ethanol solution, and the enzyme solution pool is used to hold an enzyme solution.

[0009] In a possible implementation, the positioning frame includes: a first positioning plate, and a second positioning plate connected to the first positioning plate through multiple groups of connecting rods, the first positioning plate and the second positioning plate are parallel to each other, the first positioning plate has multiple groups of first positioning holes, the second positioning plate has multiple groups of second positioning holes, and the magnetic stirrer has multiple groups of stirring points; wherein each group of the first positioning holes corresponds to a group of the second positioning holes and a group of the stirring points, and the three are coaxially arranged, so that the reaction bottle is located at the center of the stirring points when placed in the first positioning holes and the second positioning holes.

[0010] In a possible implementation, the pipette includes:

[0011] A pipette body mounted on the pipette chuck; and

[0012] A disposable gun head is quickly detachably connected to the gun body of the pipette.

[0013] In a possible implementation, the disposable gun tip module includes:

[0014] a first orifice plate mounted on the stand; and

[0015] A plurality of groups of first placement holes are provided on the first hole plate;

[0016] Wherein, the first placement hole is used to place unused disposable gun tips.

[0017] In a possible implementation, the centrifuge tube module includes:

[0018] a second orifice plate mounted on the stand; and

[0019] A plurality of groups of second placement holes are provided on the second hole plate;

[0020] Wherein, the second placement hole is used for placing a centrifuge tube.

[0021] In a possible implementation, the discarded gun tip box is in the shape of a rectangular parallelepiped with an open top, and is used to place used disposable gun tips; the electrical control cabinet is electrically connected to the water bath, the magnetic stirrer, and the pipetting mechanism.

[0022] In a possible implementation, the filter screen extends from the mouth of the reaction bottle toward the inner bottom thereof and protrudes to the middle of the reaction bottle.

[0023] In a possible implementation, the filter is located in the middle of the reaction bottle.

[0024] The beneficial effects of the technical solution provided by this application include at least:

[0025] The present application is provided with a water bath, a positioning frame installed in the water bath, a magnetic stirrer fixed at the bottom of the positioning frame, a reaction bottle placed on the positioning frame and corresponding to the stirring point of the magnetic stirrer, a liquid transfer mechanism, a disposable gun head module, a discarded gun head box, a centrifuge tube module, an ethanol pool, an enzyme liquid pool, and an electric control cabinet; wherein the liquid transfer mechanism includes a mechanical arm installed on a workbench, a liquid transfer gun chuck installed on the mechanical arm, and multiple groups of liquid transfer guns installed on the liquid transfer gun chuck; and a filter is provided in the reaction bottle. In this case, a high-throughput, high-precision, and high-sensitivity rapid detection of samples in the experimental process for predicting the glycemic index of rice based on an in vitro static digestion method is achieved, and the experimental cost is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The accompanying drawings are used to provide a further understanding of the present application and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the present application and do not constitute a limitation of the present application. In the accompanying drawings:

[0027] Figure 1A schematic diagram of the structure of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown;

[0028] Figure 2 A schematic structural diagram of a positioning frame and a magnetic stirrer of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown;

[0029] Figure 3 A schematic diagram of the structure of a liquid transfer mechanism of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown;

[0030] Figure 4 A schematic diagram of the structure of a disposable gun tip module of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown;

[0031] Figure 5 A schematic diagram of the structure of a centrifuge tube module of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown;

[0032] Figure 6 A schematic diagram of the structure of a discarded gun tip box of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown;

[0033] Figure 7 A schematic diagram of the structure of a first reaction bottle of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown;

[0034] Figure 8 A schematic diagram showing the structure of a second reaction bottle of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown;

[0035] In the figure:

[0036] 1. Workbench; 2. Water bath; 3. Positioning rack; 4. Magnetic stirrer; 5. Reaction bottle; 6. Pipetting mechanism; 7. Stand; 8. Disposable gun tip module; 9. Discarded gun tip box; 10. Centrifuge tube module; 11. Ethanol pool; 12. Enzyme liquid pool; 13. Electric control cabinet;

[0037] 31. first positioning plate; 32. connecting rod; 33. second positioning plate; 311. first positioning hole; 331. second positioning hole;

[0038] 51. Filter;

[0039] 61. Robotic arm; 62. Pipette chuck; 63. Pipette; 631. Pipette body; 632. Disposable pipette tip;

[0040] 81. a first orifice plate; 82. a first placement hole;

[0041] 101. A second orifice plate; 102. A second placement hole; 103. A centrifuge tube. DETAILED DESCRIPTION

[0042] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0043] Among them, the same parts are represented by the same figure marks. It should be noted that the words "front", "rear", "left", "right", "up" and "down" used in the following description refer to the directions in the drawings of the present application specification, and the words "bottom surface" and "top surface", "inside" and "outside" refer to the direction towards or away from a specific component, respectively. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present application specification, the meaning of "multiple" is two or more.

[0044] The present application is further described below in conjunction with the accompanying drawings and embodiments.

[0045] Figure 1 The schematic diagram of the structure of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown. The in vitro digestion device for predicting the glycemic index of rice comprises: a workbench 1, a water bath 2 installed on the workbench 1, a positioning frame 3 installed in the water bath 2, a magnetic stirrer 4 fixed to the bottom of the positioning frame 3, a reaction bottle 5 placed on the positioning frame 3 and corresponding to the stirring point of the magnetic stirrer 4, a pipetting mechanism 6 installed on the workbench 1 and located on one side of the water bath 2, A stand 7 installed on the workbench 1 and located on the other side of the water bath 2, a disposable gun tip module 8 installed on the stand 7, a discarded gun tip box 9 installed on the stand 7, a centrifuge tube module 10 installed on the stand 7, an ethanol pool 11 installed on the stand 7, an enzyme solution pool 12 installed on the stand 7, and an electric control cabinet 13 installed on the workbench 1 and located on one side of the stand 7; wherein the reaction bottle 5 is used to hold the rice grain solution after cooking, the ethanol pool 11 is used to hold the ethanol solution, and the enzyme solution pool 12 is used to hold the enzyme solution.

[0046] In the embodiment of the present application, the electric control cabinet 13 is electrically connected to the water bath 2, the magnetic stirrer 4, and the liquid transfer mechanism 6 to realize power supply and coordinated operation of each component.

[0047] In the embodiment of the present application, the magnetic stirrer 4 is a porous plate magnetic stirrer, which is specially used for stirring the porous plate, and has a plurality of stirring points evenly spaced and distributed, and can simultaneously and efficiently stir a plurality of small-volume samples in the plate. That is, efficient stirring of a plurality of groups of reaction bottles 5 on the positioning rack 3 is achieved.

[0048] In the embodiment of the present application, the water bath 2 is used to control the temperature of the digestion process. The water bath 2 is equipped with a digital temperature control system. The required temperature can be set through the panel. The system automatically controls the heating element to keep the water temperature near the set value with an accuracy of up to ±0.1°C to ±1°C.

[0049] In detail, Figure 2 A schematic structural diagram of a positioning frame and a magnetic stirrer of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown. The positioning frame 3 includes: a first positioning plate 31, and a second positioning plate 33 connected to the first positioning plate 31 through multiple groups of connecting rods 32. The first positioning plate 31 and the second positioning plate 33 are parallel to each other. The first positioning plate 31 has multiple groups of first positioning holes 311, and the second positioning plate 33 has multiple groups of second positioning holes 331. The magnetic stirrer 4 has multiple groups of stirring points; wherein each group of first positioning holes 311 corresponds to a group of second positioning holes 331 and a group of stirring points, and the three are coaxially arranged, so that the reaction bottle 5 is located at the center of the stirring points when placed in the first positioning holes 311 and the second positioning holes 331.

[0050] In the embodiment of the present application, the positioning frame 3 is used to fix the position of the magnetic stirrer 4 in the water bath 2 and the position of the reaction bottle 5 on the fixed magnetic stirrer 4, and at the same time facilitates the positioning of the pipetting mechanism 6.

[0051] Further, Figure 3 The schematic diagram of the structure of the liquid transfer mechanism of the in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown. The liquid transfer mechanism 6 comprises: a mechanical arm 61 mounted on the workbench 1, a liquid transfer gun chuck 62 mounted on the mechanical arm 61, and a plurality of groups of liquid transfer guns 63 mounted on the liquid transfer gun chuck 62. The liquid transfer gun 63 comprises: a liquid transfer gun body 631 mounted on the liquid transfer gun chuck 62, and a disposable gun head 632 quickly connected to the liquid transfer gun body 631.

[0052] In the embodiment of the present application, the pipette 63 is installed with a disposable gun tip 632 by pressing down and then snapping in. After use, the disposable gun tip 632 is ejected by the ejection mechanism of the pipette 63 to complete the removal.

[0053] Specifically, Figure 4 A schematic structural diagram of a disposable gun tip module of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown. The disposable gun tip module 8 includes: a first orifice plate 81 installed on a stand 7, and a plurality of groups of first placement holes 82 opened on the first orifice plate 81; wherein the first placement holes 82 are used to place unused disposable gun tips 632.

[0054] In the embodiment of the present application, the first well plate 81 is a non-standard 96-well plate.

[0055] Furthermore, Figure 5 A schematic structural diagram of a centrifuge tube module of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown, wherein the centrifuge tube module 10 comprises: a second orifice plate 101 mounted on a stand 7, and a plurality of groups of second placement holes 102 opened on the second orifice plate 101; wherein the second placement holes 102 are used for placing centrifuge tubes 103.

[0056] In the embodiment of the present application, the second well plate 101 includes two layers of identical 96-well plates for placing the above-mentioned centrifuge tubes 103 .

[0057] In some embodiments, Figure 6 A schematic structural diagram of a discarded gun tip box of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown. The discarded gun tip box 9 is in the shape of a rectangular parallelepiped with an open top and is used to place used disposable gun tips 632.

[0058] As a preferred embodiment, the reaction bottle 5 has a filter 51. In one example, Figure 7 The schematic diagram of the structure of the first reaction bottle of the in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown, and the filter 51 extends from the bottle mouth of the reaction bottle 5 to the inner bottom thereof and protrudes to the middle of the reaction bottle 5. In another example, Figure 8 A schematic structural diagram of a second reaction bottle of an in vitro digestion device for predicting the glycemic index of rice provided by an exemplary embodiment of the present application is shown, and the filter screen 51 is located in the middle of the reaction bottle 5 .

[0059] In the embodiment of the present application, the filter 51 can be provided to avoid sampling errors caused by large particles clogging the gun tip or inhaling small particles when taking liquid.

[0060] In order to better understand the present application, the working principle of the in vitro digestion device for predicting the glycemic index of rice involved in the embodiments of the present application is explained below.

[0061] Preliminary experimental preparation operations:

[0062] Step 1: Turn on the water bath 2 to ensure a constant temperature during the digestion experiment;

[0063] Step 2: Place multiple groups of reaction bottles 5 containing cooked rice grain solution on the positioning frame 3, at the center of each stirring position of the magnetic stirrer 4, turn on the magnetic stirrer 4, and set the stirrer speed;

[0064] Step 3: The pipetting mechanism 6 drives the pipetting gun 63 to move to the position of the disposable gun tip module 8, and installs a new disposable gun tip 632 for the pipetting gun 63;

[0065] Step 4: the pipetting mechanism 6 drives the pipetting gun 63 to transfer a certain amount of ethanol solution from the ethanol pool 11 and add it to the multiple groups of centrifuge tubes 103 placed in the centrifuge tube module 10;

[0066] Step 5, repeat step 4 until all centrifuge tubes 103 are filled with ethanol solution;

[0067] Step 6: the pipetting mechanism 6 drives the pipetting gun 63 to move to the discarded gun tip box 9 and ejects the used disposable gun tip 632 and discards it in the discarded gun tip box 9;

[0068] Experimental formal operation:

[0069] Step 7: The pipetting mechanism 6 drives the pipetting gun 63 to move to the position of the disposable gun tip module 8, and installs a new disposable gun tip 632 for the pipetting gun 63;

[0070] Step eight, the pipetting mechanism 6 drives the pipette gun 63 to move to the reaction bottle 5 in the water bath 2, and a certain amount of the unreacted cooked rice grain solution is taken from the reaction bottle 5 by the pipette gun 63;

[0071] Step nine, the pipetting mechanism 6 drives the pipetting gun 63 to transfer the unreacted cooked rice grain solution obtained in step eight to the centrifuge tube 103 on the centrifuge tube module 10 to which the ethanol solution has been added;

[0072] Step 10, the pipetting mechanism 6 drives the pipetting gun 63 to move to the discarded gun tip box 9 and ejects the used disposable gun tip 632 and discards it in the discarded gun tip box 9;

[0073] Step 11: the pipetting mechanism 6 drives the pipetting gun 63 to move to the position of the disposable gun tip module 8, and installs a new disposable gun tip 632 for the pipetting gun 63;

[0074] Step 12: the pipetting mechanism 6 drives the pipetting gun 63 to move to the enzyme solution pool 12 to take a certain amount of enzyme solution;

[0075] Step 13, the pipetting mechanism 6 drives the pipetting gun 63 to move to the reaction bottle 5 in the water bath 2, and the enzyme solution obtained in step 12 is added to the reaction bottle 5. After the enzyme solution is added, the rice digestion reaction in the reaction bottle 5 begins, and this time is recorded as 0 min when the rice digestion reaction starts;

[0076] Step 14: the pipetting mechanism 6 drives the pipetting gun 63 to move to the discarded gun tip box 9 and ejects the used disposable gun tip 632 and discards it in the discarded gun tip box 9;

[0077] Step 15: the pipetting mechanism 6 drives the pipetting gun 63 to move to the position of the disposable gun tip module 8, and installs a new disposable gun tip 632 for the pipetting gun 63;

[0078] Step 16: 5 minutes after the rice digestion reaction starts, the pipetting mechanism 6 drives the pipetting gun 63 to move to the reaction bottle 5, and takes a certain amount of reaction solution from the reaction bottle 5;

[0079] Step 17: the pipetting mechanism 6 drives the pipetting gun 63 to transfer the reaction solution obtained in step 16 to the centrifuge tube 103 on the centrifuge tube module 10 to which the ethanol solution has been added;

[0080] Step 18, the pipetting mechanism 6 drives the pipetting gun 63 to move to the discarded gun tip box 9 and ejects the used disposable gun tip 632 and discards it in the discarded gun tip box 9;

[0081] Step 19, repeating steps 15 to 18 at different time points (10 min, 15 min, 20 min, 30 min, 45 min, 60 min, 90 min, 120 min, 180 min) after the start of the rice digestion reaction, and after the last repeated operation is completed after 180 min, several groups of digestion product samples are obtained, and the rice digestion reaction experiment is completed.

[0082] In summary, the present application is provided with a water bath, a positioning frame installed in the water bath, a magnetic stirrer fixed at the bottom of the positioning frame, a reaction bottle placed on the positioning frame and corresponding to the stirring point of the magnetic stirrer, a liquid transfer mechanism, a disposable gun head module, a discarded gun head box, a centrifuge tube module, an ethanol pool, an enzyme liquid pool, and an electric control cabinet; wherein the liquid transfer mechanism includes a mechanical arm installed on a workbench, a liquid transfer gun chuck installed on the mechanical arm, and a plurality of groups of liquid transfer guns installed on the liquid transfer gun chuck; and a filter is provided in the reaction bottle. In this case, a high-throughput, high-precision, and high-sensitivity rapid detection of samples in the experimental process for predicting the glycemic index of rice based on an in vitro static digestion method is achieved, and the experimental cost is reduced.

[0083] In the embodiments disclosed in the present application, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; "connected" can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments disclosed in the present utility model can be understood according to specific circumstances.

[0084] The above is only a preferred implementation of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present application. These improvements and modifications should also be regarded as the scope of protection of the present application.

Claims

1. An in vitro digestion device for predicting the glycemic index of rice, characterized in that: include: A workbench, a water bath installed on the workbench, a positioning frame installed in the water bath, a magnetic stirrer fixed at the bottom of the positioning frame, a reaction bottle placed on the positioning frame and corresponding to the stirring point of the magnetic stirrer, a pipetting mechanism installed on the workbench and located on one side of the water bath, a stand installed on the workbench and located on the other side of the water bath, a disposable gun tip module installed on the stand, a discarded gun tip box installed on the stand, a centrifuge tube module installed on the stand, an ethanol pool installed on the stand, an enzyme liquid pool installed on the stand, and an electric control cabinet installed on the workbench and located on one side of the stand; the pipetting mechanism includes a mechanical arm installed on the workbench, a pipette gun chuck installed on the mechanical arm, and a plurality of groups of pipette guns installed on the pipette gun chuck; a filter is provided in the reaction bottle; The reaction bottle is used to hold the cooked rice grain solution, the ethanol pool is used to hold the ethanol solution, and the enzyme solution pool is used to hold the enzyme solution.

2. The in vitro digestion device for predicting the glycemic index of rice according to claim 1, characterized in that: The positioning frame comprises: a first positioning plate, and a second positioning plate connected to the first positioning plate through multiple groups of connecting rods, the first positioning plate and the second positioning plate are parallel to each other, the first positioning plate has multiple groups of first positioning holes, the second positioning plate has multiple groups of second positioning holes, and the magnetic stirrer has multiple groups of stirring points; Among them, each group of the first positioning holes corresponds to a group of the second positioning holes and a group of the stirring points, and the three are coaxially arranged, so that the reaction bottle is located at the center of the stirring points when placed in the first positioning holes and the second positioning holes.

3. The in vitro digestion device for predicting the glycemic index of rice according to claim 1, characterized in that: The pipette comprises: A pipette body mounted on the pipette chuck; and A disposable gun head is quickly detachably connected to the gun body of the pipette.

4. The in vitro digestion device for predicting the glycemic index of rice according to claim 1, characterized in that: The disposable gun tip module comprises: a first orifice plate mounted on the stand; and A plurality of groups of first placement holes are provided on the first hole plate; Wherein, the first placement hole is used to place unused disposable gun tips.

5. The in vitro digestion device for predicting the glycemic index of rice according to claim 1, characterized in that: The centrifuge tube module comprises: a second orifice plate mounted on the stand; and A plurality of groups of second placement holes are provided on the second hole plate; Wherein, the second placement hole is used for placing a centrifuge tube.

6. The in vitro digestion device for predicting the glycemic index of rice according to claim 1, characterized in that: The discarded gun tip box is in the shape of a rectangular parallelepiped with an open top, and is used to store used disposable gun tips; The electric control cabinet is electrically connected to the water bath, the magnetic stirrer, and the liquid transfer mechanism.

7. The in vitro digestion device for predicting the glycemic index of rice according to claim 1, characterized in that: The filter screen extends from the mouth of the reaction bottle to the inner bottom thereof and protrudes to the middle of the reaction bottle.

8. The in vitro digestion device for predicting the glycemic index of rice according to claim 1, characterized in that: The filter is located in the middle of the reaction bottle.

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