Novel electrophoresis gel divider

Abstract

The utility model discloses a novel electrophoresis gel divider which comprises a base, a boss is arranged in the middle of the upper surface of the base, two guide rails are fixed to the positions, located on the two sides of the boss, of the upper surface of the base, sliding sleeves are connected to the surfaces of the two guide rails in a sliding mode, and a portal frame is jointly and fixedly connected to the upper surfaces of the two sliding sleeves. A vertical rod penetrates through and is slidably connected to the middle of the top end of the portal frame. The base, the boss, the two guide rails, the two sliding sleeves, the portal frame, the U-shaped plate, the penetrating hole, the cutting line and the line fixing assembly are arranged, the portal frame can move along the two guide rails through the two sliding sleeves, the scale marks for positioning the positions of the sliding sleeves are arranged on the surface of the base, and when the U-shaped plate and the cutting line move to the accurate positions along with the portal frame, the U-shaped plate and the cutting line can be fixed. The electrophoresis gel can be accurately cut by pressing down the U-shaped plate and the cutting line, when the cutter is used for cutting the gel, it can be guaranteed that a cut is neat, and gel breakage and inaccurate cutting are avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of electrophoresis gel cutting, in particular to a novel electrophoresis gel splitter. Background Art

[0002] Protein immunoblotting is one of the classic and mature experimental techniques in molecular biology. Through protein extraction, electrophoresis, membrane transfer, exposure and color development, proteins are separated according to molecular weight and the expression of target proteins is specifically detected. After electrophoresis, it is usually necessary to divide the polyacrylamide gel into strips according to the molecular weight of the target protein, and then continue with the membrane transfer process.

[0003] However, since the gel needs to be cut in the electrophoresis solution and the gel is brittle and easy to crack, using a traditional blade to cut may cause gel breakage, inaccurate cutting, and miscutting of the target protein. Therefore, in order to standardize the experimental steps and reduce operational errors, the utility model proposes a new electrophoresis gel splitter. Utility Model Content

[0004] The utility model aims to solve the shortcomings in the prior art and proposes a novel electrophoresis gel splitter.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a novel electrophoresis gel splitter, comprising a base, a boss is provided in the middle of the upper surface of the base, two guide rails are fixed on the upper surface of the base and on both sides of the boss, the surfaces of the two guide rails are slidably connected with sliding sleeves, the upper surfaces of the two sliding sleeves are commonly fixedly connected with a gantry, a vertical rod passes through the middle of the top of the gantry and is slidably connected, the bottom end of the vertical rod is fixedly connected with a U-shaped plate, the bottom ends of both sides of the U-shaped plate are provided with through holes, cutting lines are commonly penetrated inside the two through holes, fixed line components are provided on both sides of the U-shaped plate, pointers are fixed on the outer walls of the two sliding sleeves, and scale lines are provided on the upper surface of the base and close to the pointer.

[0006] Furthermore, the guide rail has a T-shaped cross-section, and a plurality of grooves are provided on the inner top wall of the sliding sleeve. Rollers are rotatably connected inside the grooves, and the bottom ends of the rollers are in contact with the upper surface of the guide rail.

[0007] Furthermore, baffles are fixedly connected to both ends of the guide rail.

[0008] Furthermore, a locking bolt passes through and is threadedly connected to the top wall of the sliding sleeve.

[0009] Furthermore, the wire fixing assembly includes two side plates fixed to the side walls of the U-shaped plate, the two side plates are respectively located below and above the through hole, the middle part of the side plate located above the through hole is penetrated by and threadedly connected with an extrusion bolt, the bottom end of the extrusion bolt is fixedly connected to a pressure plate, and the cutting line is located between the two side plates.

[0010] Furthermore, a handle is fixedly connected to the top of the vertical rod.

[0011] Furthermore, guide rods are fixedly connected to both sides of the upper surface of the U-shaped plate, and the two guide rods penetrate the top wall of the gantry and are slidably connected to the gantry. The upper surface of the U-shaped plate and the top wall of the gantry are fixedly connected with a spring.

[0012] Beneficial effects of the utility model:

[0013] When the utility model is in use, the novel electrophoresis gel splitter is provided with a base, a boss, two guide rails, two sliding sleeves, a gantry, a U-shaped plate, perforations, a cutting line and a fixing line component; the gantry can move along the two guide rails through the two sliding sleeves, and the surface of the base is provided with scale lines for positioning the position of the sliding sleeves; when the U-shaped plate and the cutting line follow the gantry to move to a precise position, the electrophoresis gel can be accurately cut by pressing down the U-shaped plate and the cutting line; using the splitter to cut the gel can ensure a neat incision and avoid gel rupture and inaccurate cutting. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solution of the present invention, the drawings required for use in the description of the specific implementation methods will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 : The overall stereogram of the utility model;

[0016] Figure 2 : An overall cross-sectional view of the utility model;

[0017] Figure 3 :The utility model Figure 2 Enlarged view of point A in the middle;

[0018] Figure 4 :The utility model Figure 2 Enlarged view of point B in the middle.

[0019] The reference numerals are as follows:

[0020] 1. Base; 101. Boss; 2. Guide rail; 3. Sleeve; 4. Gantry; 5. Vertical bar; 6. U-shaped plate; 61. Perforation; 7. Cutting line; 8. Wire fixing assembly; 81. Side plate; 82. Extrusion bolt; 9. Guide rod; 10. Spring; 11. Handle; 12. Groove; 13. Roller; 14. Locking bolt; 15. Baffle; 16. Pointer; 17. Scale line. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] like Figure 1-Figure 4 As shown, it relates to a novel electrophoresis gel splitter, including a base 1, a boss 101 is provided in the middle of the upper surface of the base 1, two guide rails 2 are fixed on the upper surface of the base 1 and on both sides of the boss 101, the surfaces of the two guide rails 2 are slidably connected with sliding sleeves 3, the upper surfaces of the two sliding sleeves 3 are commonly fixedly connected with a gantry 4, a vertical rod 5 passes through and slidably connected to the middle of the top of the gantry 4, a U-shaped plate 6 is fixedly connected to the bottom end of the vertical rod 5, the bottom ends of both sides of the U-shaped plate 6 are provided with through holes 61, cutting lines 7 are commonly penetrated inside the two through holes 61, and line fixing components 8 are provided on both sides of the U-shaped plate 6, pointers 16 are fixed to the outer walls of the two sliding sleeves 3, and scale lines 17 are provided on the upper surface of the base 1 and close to the pointer 16.

[0023] like Figure 1-Figure 4 As shown, the guide rail 2 has a T-shaped cross section, and a plurality of grooves 12 are provided on the inner top wall of the sliding sleeve 3 . A roller 13 is rotatably connected inside the groove 12 , and the bottom end of the roller 13 is in contact with the upper surface of the guide rail 2 .

[0024] The guide rail 2 is designed to be T-shaped, and the sleeve 3 is C-shaped, which can prevent the sleeve 3 from being separated from the guide rail 2, and the roller 13 can reduce the friction force of the sleeve 3 moving on the surface of the guide rail 2.

[0025] like Figure 1 and Figure 4 As shown, baffles 15 are fixedly connected to both ends of the guide rail 2.

[0026] By providing the baffle 15 , the sliding sleeve 3 can be prevented from being separated from the guide rail 2 when moving toward the two ends of the guide rail 2 .

[0027] like Figure 4 As shown, a locking bolt 14 penetrates through the top wall of the sliding sleeve 3 and is threadedly connected.

[0028] When the sliding sleeve 3 is moved to any position on the surface of the guide rail 2 , the sliding sleeve 3 can be fixed by tightening the locking bolt 14 .

[0029] like Figure 1-Figure 3As shown, the wire fixing assembly 8 includes two side plates 81 fixed to the side walls of the U-shaped plate 6, and the two side plates 81 are respectively located below and above the through hole 61. An extrusion bolt 82 is passed through the middle of the side plate 81 located above the through hole 61 and is threadedly connected. The bottom end of the extrusion bolt 82 is fixedly connected to a pressure plate, and the cutting line 7 is located between the two side plates 81.

[0030] In this embodiment, the cutting line 7 adopts a high-tension line. The two ends of the cutting line 7 pass through the through holes 61 on both sides of the U-shaped plate 6 and are located between the two side plates 81 at both ends. Then, the pressing plate is driven to squeeze and fix the cutting line 7 by tightening the extrusion bolt 82. Therefore, when the cutting line 7 breaks, the cutting line 7 can be disassembled and replaced by loosening the extrusion bolt 82.

[0031] A handle 11 is fixedly connected to the top of the vertical rod 5 .

[0032] The handle 11 can be used to press the vertical rod 5 downward, thereby driving the U-shaped plate 6 and the cutting line 7 to descend, and the cutting line 7 is used to cut the gel on the surface of the boss 101.

[0033] like Figure 1-Figure 2 As shown, guide rods 9 are fixedly connected to both sides of the upper surface of the U-shaped plate 6. The two guide rods 9 penetrate the top wall of the gantry 4 and are slidably connected to the gantry 4. A spring 10 is fixedly connected to the upper surface of the U-shaped plate 6 and the top wall of the gantry 4.

[0034] When the handle 11 is released, the spring 10 will drive the U-shaped plate 6 to automatically rise and reset, and the guide rod 9 will play a role in stabilizing and guiding the up and down movement of the U-shaped plate 6.

[0035] Working principle: put the gel on the surface of the boss 101, then move the gantry 4, the sliding sleeve 3 at the bottom of the gantry 4 will move along the guide rail 2, and the position of the gantry 4 and the cutting line 7 can be accurately located by observing the position of the scale line 17 aligned with the pointer 16 on the side wall of the sliding sleeve 3. When it moves to the specified position, tighten the locking bolt 14 to fix the sliding sleeve 3, and then press the vertical rod 5 down through the handle 11, thereby driving the U-shaped plate 6 and the cutting line 7 to descend, so as to realize the cutting of the gel by the cutting line 7. After the cutting is completed, release the handle 11, and the spring 10 drives the U-shaped plate 6 to rise and reset. When cutting the next place, loosen the locking bolt 14 and continue to move the gantry 4 to the next position, and then cut.

[0036] The preferred embodiments of the utility model disclosed above are only used to help explain the utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the utility model to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the utility model, so that technicians in the relevant technical field can well understand and use the utility model. The utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A novel electrophoresis gel splitter, comprising a base (1), characterized in that: A boss (101) is provided in the middle of the upper surface of the base (1), two guide rails (2) are fixed on the upper surface of the base (1) and located on both sides of the boss (101), the surfaces of the two guide rails (2) are slidably connected with a sliding sleeve (3), the upper surfaces of the two sliding sleeves (3) are commonly fixedly connected with a gantry (4), a vertical rod (5) passes through the middle of the top of the gantry (4) and is slidably connected, the bottom end of the vertical rod (5) is fixedly connected with a U-shaped plate (6), the bottom ends of both sides of the U-shaped plate (6) are provided with a through hole (61), the insides of the two through holes (61) are commonly penetrated with a cutting line (7), both sides of the U-shaped plate (6) are provided with a line fixing component (8), the outer side walls of the two sliding sleeves (3) are fixed with a pointer (16), and a scale line (17) is provided on the upper surface of the base (1) and close to the pointer (16).

2. A novel electrophoresis gel splitter according to claim 1, characterized in that: The guide rail (2) has a T-shaped cross section, and a plurality of grooves (12) are provided on the inner top wall of the sliding sleeve (3). A roller (13) is rotatably connected inside the groove (12), and the bottom end of the roller (13) is in contact with the upper surface of the guide rail (2).

3. A novel electrophoresis gel splitter according to claim 1, characterized in that: Baffles (15) are fixedly connected to both ends of the guide rail (2).

4. A novel electrophoresis gel splitter according to claim 1, characterized in that: A locking bolt (14) passes through the top wall of the sliding sleeve (3) and is threadedly connected thereto.

5. A novel electrophoresis gel splitter according to claim 1, characterized in that: The wire fixing assembly (8) comprises two side plates (81) fixed to the side wall of the U-shaped plate (6), the two side plates (81) being respectively located below and above the through hole (61), a squeezing bolt (82) passing through and threadedly connected to the middle of the side plate (81) located above the through hole (61), a pressing plate being fixedly connected to the bottom end of the squeezing bolt (82), and the cutting line (7) being located between the two side plates (81).

6. A novel electrophoresis gel splitter according to claim 1, characterized in that: A handle (11) is fixedly connected to the top of the vertical rod (5).

7. A novel electrophoresis gel splitter according to claim 1, characterized in that: Guide rods (9) are fixedly connected to both sides of the upper surface of the U-shaped plate (6); the two guide rods (9) penetrate the top wall of the gantry (4) and are slidably connected to the gantry (4); and a spring (10) is fixedly connected to the upper surface of the U-shaped plate (6) and the top wall of the gantry (4).

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