Immunochromatographic analysis system

Abstract

The utility model discloses an immunochromatography analysis system. The system comprises a light source, a light source optical filter, a focusing lens, a fluorescent optical filter, a temperature control system and a photoelectric signal conversion and data analysis calculation display system, a kit is placed on the temperature control system, and the temperature control system is used for controlling the environment temperature of chromatographic reaction of a test strip in the kit; light emitted by the light source passes through the light source optical filter and then irradiates an observation window area of the kit to excite a T line and a C line in the kit to generate fluorescent light; the generated fluorescent light sequentially passes through the focusing lens and the fluorescent light filter and then is received by the photoelectric signal conversion and data analysis and calculation display system, and a detection result is output after data analysis and calculation. The system has the advantages of being small in size, low in cost, convenient to operate, high in sensitivity, good in stability and the like.

Description

Technical Field

[0001] The utility model belongs to the technical field of immunoassay detection, in particular to an immunochromatographic analysis system. Background Art

[0002] The immunofluorescence analysis system, the basic reaction is antigen-antibody reaction, used to quantitatively detect the virus concentration of the sample, and comprehensively judge the test results by the brightness of the T line (test line) and the C line (quality control line). At present, the optical detection system of the common immunofluorescence analysis system uses a photodiode to receive signals. The optical detection system is fixed and cannot detect two lines at the same time, so a mobile scanning structure is used to detect the T line and the C line respectively; the optical path system uses a two-way mirror splitting solution, which is high in cost, complex in structure, large in installation space, and requires high installation precision.

[0003] Chinese patent application CN1766579A "Upconversion phosphorescent immunochromatographic test strip detection system" makes improvements. The optical path still uses a dichroic mirror splitting solution; the light beam emitted by the light source is divided into two beams through the optical fiber. The two beams correspond to the T line and C line of the test kit respectively, and the two lines can be illuminated at the same time. The component used to receive and convert the fluorescent signal is a camera, which can shoot the two lines at the same time. This method eliminates the step of moving the test kit, simplifies the system, and reduces the failure rate. However, the problems brought by the above scheme are: 1. The inconsistency in the production and installation of the optical fiber leads to differences in the intensity of the two beams of light, and the illumination intensity of the T line and the C line is inconsistent, which affects the accuracy of the detection; 2. The absorption and reflection of fluorescence by the dichroic mirror reduces the sensitivity of the optical detection system; 3. There are differences between the sizes of different test kits. The position of the T line and the C line will change relative to the light beam each time, resulting in reduced accuracy. Utility Model Content

[0004] The utility model aims to provide an immunochromatographic analysis system, which has the characteristics of small size, low cost, easy operation, high sensitivity and good stability.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] An immunochromatographic analysis system, comprising a light source, a light source filter, a focusing lens, a fluorescent filter, a temperature control system, a photoelectric signal conversion and a data analysis calculation and display system;

[0007] The test kit is placed on the temperature control system to control the ambient temperature of the test strip chromatography reaction in the test kit;

[0008] The light emitted by the light source passes through the light source filter and then irradiates the observation window area of ​​the reagent kit, exciting the T line and C line in the reagent kit to generate fluorescence; the generated fluorescence passes through the focusing lens and the fluorescence filter in turn, and is collected by the photoelectric signal conversion and data analysis calculation and display system and converted into other signals that can be analyzed and calculated by the system, and the detection result is output after data analysis and calculation.

[0009] Furthermore, the immunochromatographic analysis system also includes a reflector, which is arranged on the light path between the light source and the reagent box. The light emitted by the light source passes through the light source filter and is reflected by the reflector to illuminate the observation window area of ​​the reagent box.

[0010] Preferably, the photoelectric signal conversion and data analysis calculation and display system includes a photoelectric signal acquisition and conversion system, a reagent kit identification recognition system, a data analysis calculation system, a communication control system and a display system; the photoelectric signal acquisition and conversion system acquires the fluorescent signal image generated by the stimulation in the reagent kit and after conversion, transmits it to the data analysis calculation system, and after data analysis and calculation, transmits it to the communication control system; the reagent kit identification recognition system scans and acquires the identification on the reagent kit, and the recognition result is transmitted to the communication control system and corresponds with the data result of the data analysis calculation system, and the corresponding data is sent to the display interface of the display system.

[0011] Further preferably, the photoelectric signal acquisition and conversion system is a CCD camera, a CMOS camera or a photodiode array.

[0012] Preferably, the light source is an LED lamp, a deuterium lamp or an ultra-high pressure mercury lamp.

[0013] Preferably, the temperature control system includes a heating element, a heat conducting element, a temperature sensor and a temperature protection switch, wherein the heat conducting element is in contact with the reaction area of ​​the reagent box. The temperature control system provides a constant temperature close to the human body for the reaction inside the reagent box, which is conducive to the full reaction of the reagent and reduces the influence of temperature on the detection accuracy.

[0014] The kit identification system can identify the batch, test items, and label information of the kit by identifying the label on the kit. According to different items, the system automatically calls the corresponding program for detection and analysis. The label can be a QR code, barcode, chip, or radio frequency tag.

[0015] In the utility model, the light source is used to excite the T line and C line in the reagent box to generate fluorescence, and the photoelectric signal acquisition and conversion system adopts a camera photography method to image the fluorescence of the T line and the C line. The data analysis and calculation system analyzes the RGB values ​​of the images of the T line and the C line, and uses the brightness ratio (T / C) of the two lines to determine the fluorescence intensity. The light source and the photoelectric signal acquisition and conversion system are located above the reagent box, the light source includes a lamp bead and a filter, and the photoelectric signal acquisition and conversion system can be a CCD camera, a CMOS camera or a photodiode array.

[0016] In the present invention, since the light source is close to the detection area of ​​the test kit, there may be brightness differences between the T-line and C-line positions, affecting the detection accuracy. In order not to increase the volume of the system, a reflection scheme of the light source can be used to increase the optical path so that the brightness of the detection area tends to be consistent, such as adding a reflector in the optical path.

[0017] The photoelectric signal conversion and data analysis calculation and display system can use a device terminal that integrates a photoelectric signal acquisition and conversion system, a reagent kit identification system, a data analysis and calculation system, a communication control system and a display system, such as a mobile phone, etc., which can further simplify the structure of the immunochromatographic analysis system. In the process of using with a mobile phone, the camera in the photoelectric signal acquisition and conversion system can use the camera system of the mobile phone, the reagent kit identification system can scan the identification of the reagent kit through the mobile phone or use the NFC identification function to obtain the basic information of the tested reagent kit, and the data analysis and calculation system and the communication control system can realize the corresponding functions through the mobile phone APP.

[0018] For the pictures taken by the camera, select the 500 pixels or other several pixels with the highest brightness values ​​in the T-line and C-line areas respectively and calculate the average value, which is used as the fluorescence value of the T-line and C-line respectively.

[0019] The communication control system sends the detection results to the terminal display interface. The terminal can be a mobile phone or a display built into the system.

[0020] Because the brightness of different light sources varies greatly, the brightness of the light source needs to be calibrated before leaving the factory. The method is as follows: insert a standard fluorescence card into the system, take a photo for the first time, and collect images at the default light source brightness. If the fluorescence intensity is less than the standard range, increase the light source brightness; take photos again until the fluorescence intensity is within the standard range. If the first of two consecutive photo results is less than the standard range and the second is greater than the standard range, take the average of the two brightnesses.

[0021] Due to manufacturing and installation differences of light sources, the light intensity at the T-line and C-line positions in the detection area is not completely equal. The following scheme is used for compensation: the system is calibrated before leaving the factory, and a test card with completely equal brightness of T-line and C-line is tested. The fluorescence intensity of T-line and C-line are Rt and Rc respectively. If Rt≠Rc, change the software algorithm and introduce a coefficient k so that Rt=k*Rc to offset the uneven illumination of the light source.

[0022] The specific detection method of the utility model is: the system is turned on, the temperature control system controls the temperature at 30-37°C, the reagent box is inserted into the system after the sample is added, the system identifies the mark on the reagent box to determine the detection item and other effective information, and then controls the detection process within a corresponding determined temperature range, and starts the countdown detection according to the reagent reaction time of the detected item, after the chromatography reaction of the test strip is completed, the light source is turned on, the camera takes a picture, the image is transmitted to the data analysis and calculation system and the fluorescence value is output, and then converted into the actual sample concentration value through the corresponding detection item standard curve calculated and analyzed and stored in the system, and finally, the detection result is output to the display interface.

[0023] Compared with the prior art, the utility model has the following beneficial effects:

[0024] 1. The optical detection system has a simple structure and no moving parts, low cost and small space required.

[0025] 2. The optical detection system has low requirements on the precision of manufacturing and installation of the system, which can reduce the difficulty of manufacturing and installation.

[0026] 3. According to different test items, the optimal reaction environment temperature of the corresponding test kit is automatically selected and controlled.

[0027] 4. Calibrate the brightness of the light source before leaving the factory to ensure that the brightness of light sources in different systems is consistent. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a structural diagram of the immunochromatographic analysis system described in Example 1;

[0029] Figure 2 is a structural diagram of the immunochromatographic analysis system described in Example 2;

[0030] Figure 3 is a structural diagram of the immunochromatographic analysis system described in Example 3;

[0031] Reference numerals:

[0032] 1- temperature control system; 2- reagent box; 3- light source filter; 4- light source; 5- focusing lens; 6- fluorescence filter; 7- photoelectric signal acquisition and conversion system; 8- data analysis and calculation system; 9- communication control system; 10- display system; 11- reagent box identification system; 12- reflector; 13- mobile phone. DETAILED DESCRIPTION

[0033] The technical solution of the utility model is described in detail below with reference to the accompanying drawings and embodiments.

[0034] Example 1

[0035] like Figure 1 As shown, an immunochromatographic analysis system includes a temperature control system 1, a light source 4, a light source filter 3, a focusing lens 5, a fluorescence filter 6, a photoelectric signal acquisition and conversion system 7, a data analysis and calculation system 8, a reagent kit identification system 11, a communication control system 9 and a display system 10.

[0036] The temperature control system 1 is provided with the reagent kit 2 and controls the chromatographic reaction environment temperature of the reagent kit 2 .

[0037] The light emitted by the light source 4 passes through the light source filter 3 and then irradiates the reagent box 2 to excite the T line and C line in the reagent box 2 to generate fluorescence; the generated fluorescence passes through the focusing lens 5 and the fluorescence filter 6 in turn and is received by the photoelectric signal acquisition and conversion system 7.

[0038] The photoelectric signal acquisition and conversion system 7 acquires the fluorescent signal generated by the test kit, converts it, and inputs it into the data analysis and calculation system 8; the test kit identification recognition system 11 scans and acquires the identification information on the test kit, and the recognition result is matched with the data analysis result of the data analysis and calculation system 8 through the communication control system 9, and the matched data is sent to the display interface.

[0039] The photoelectric signal acquisition and conversion system is a CCD camera.

[0040] The light source is an LED lamp.

[0041] The temperature control system includes a heating element, a heat-conducting element, a temperature sensor and a temperature protection switch, wherein the heat-conducting element contacts the reaction area of ​​the reagent box, the heating element transfers heat to the heat-conducting element, the temperature sensor monitors and detects the temperature of the heat-conducting element, and the temperature protection switch controls the heating temperature of the heating element according to the monitoring and detection results of the temperature sensor.

[0042] Example 2

[0043] like Figure 2As shown, an immunochromatographic analysis system includes a temperature control system 1, a light source 4, a light source filter 3, a focusing lens 5, a fluorescence filter 6, a photoelectric signal acquisition and conversion system 7, a data analysis and calculation system 8, a reagent kit identification system 11, a reflector 12, a communication control system 9 and a display system 10.

[0044] The temperature control system 1 is provided with the reagent kit 2 and controls the chromatographic reaction environment temperature of the reagent kit 2;

[0045] The light emitted by the light source 4 passes through the light source filter 3, and is reflected by the reflector 12 to illuminate the observation window area of ​​the reagent box 2, so as to excite the T line and C line in the reagent box 2 and generate fluorescence; the generated fluorescence passes through the focusing lens 5 and the fluorescence filter 6 in turn and is received by the photoelectric signal acquisition and conversion system 7.

[0046] The photoelectric signal acquisition and conversion system 7 acquires the fluorescent signal generated by the test kit, converts it, and transmits it to the data analysis and calculation system 8; the test kit identification recognition system 11 scans and collects the identification on the test kit, and the recognition result is transmitted to the communication control system 9 and corresponds to the data result of the data analysis and calculation system 8, and the corresponding data is sent to the display interface.

[0047] The photoelectric signal acquisition and conversion system is a CMOS camera.

[0048] The light source is an ultra-high pressure mercury lamp.

[0049] The reflector is arranged on the optical path between the light source and the reagent box. The light emitted by the light source passes through the light source filter and is reflected by the reflector before being irradiated on the reagent box.

[0050] The temperature control system includes a heating element, a heat-conducting element, a temperature sensor and a temperature protection switch, wherein the heat-conducting element contacts the reaction area of ​​the reagent box. The heating element transfers heat to the heat-conducting element, the temperature sensor monitors and detects the temperature of the heat-conducting element, and the temperature protection switch is opened and closed according to the monitoring and detection results of the temperature sensor to control the heating temperature of the heating element.

[0051] Example 3

[0052] like Figure 3As shown, different from Example 1, in this embodiment, the photoelectric signal conversion and data analysis and calculation system uses a mobile phone (or other handheld devices or portable devices) that integrates a photoelectric signal acquisition and conversion system, a reagent kit identification and recognition system, a data analysis and calculation system, a communication control system, and a display system, so that the structure of the immunochromatography analysis system can be simplified. In the process of using with a mobile phone, the camera in the photoelectric signal acquisition and conversion system can use the camera that comes with the mobile phone, the reagent kit identification and recognition system can scan the QR code of the reagent kit through the mobile phone or identify the item information of the detected reagent through NFC, and the data analysis and calculation system and the communication control system can use a mobile phone APP to implement the corresponding functions.

[0053] The components in the system of the utility model can be purchased.

[0054] Any content not described in detail in the present invention can be based on the conventional technical knowledge in the field.

[0055] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model and are not intended to limit it. Although the utility model is described in detail with reference to the embodiments, a person skilled in the art should understand that any modification or equivalent replacement of the technical solution of the utility model does not deviate from the spirit and scope of the technical solution of the utility model, and should be included in the scope of the claims of the utility model.

Claims

1. An immunochromatographic analysis system, characterized in that: The immunochromatographic analysis system includes a light source, a light source filter, a focusing lens, a fluorescence filter, a temperature control system, a photoelectric signal conversion and a data analysis calculation and display system; The test kit is placed on the temperature control system to control the ambient temperature of the test strip chromatography reaction in the test kit; The light emitted by the light source passes through the light source filter and then illuminates the observation window area of ​​the reagent kit, exciting the T line and C line in the reagent kit to generate fluorescence; the generated fluorescence passes through the focusing lens and the fluorescence filter in turn and is received by the photoelectric signal conversion and data analysis calculation and display system, and the detection result is output after data analysis and calculation.

2. The immunochromatographic analysis system according to claim 1, characterized in that: The immunochromatographic analysis system further comprises a reflector, which is arranged on the optical path between the light source and the reagent box. The light emitted by the light source passes through the light source filter and is then reflected by the reflector and irradiated onto the reagent box observation window area.

3. The immunochromatographic analysis system according to claim 1 or 2, characterized in that: The photoelectric signal conversion and data analysis calculation and display system includes a photoelectric signal acquisition and conversion system, a reagent kit identification recognition system, a data analysis calculation system, a communication control system and a display system; the photoelectric signal acquisition and conversion system acquires the fluorescent signal generated in the reagent kit and converts it, and then transmits it to the data analysis calculation system, and then transmits it to the communication control system after data analysis and calculation; the reagent kit identification recognition system scans and acquires the identification information on the reagent kit, and the recognition result is transmitted to the communication control system and corresponds to the data result of the data analysis calculation system, and the corresponding data is sent to the display system.

4. The immunochromatographic analysis system according to claim 3, characterized in that: The photoelectric signal acquisition and conversion system is a CCD camera, a CMOS camera or a photodiode array.

5. The immunochromatographic analysis system according to claim 3, characterized in that: The reagent kit is identified by a QR code, a barcode, a chip or a radio frequency tag.

6. The immunochromatographic analysis system according to claim 1 or 2, characterized in that: The light source is an LED lamp, a deuterium lamp or an ultra-high pressure mercury lamp.

7. The immunochromatographic analysis system according to claim 1 or 2, characterized in that: The temperature control system includes a heating element, a heat-conducting element, a temperature sensor and a temperature protection switch. The heat-conducting element contacts the reaction area of ​​the reagent box. The heating element transfers heat to the heat-conducting element. The temperature sensor monitors and detects the temperature of the heat-conducting element. The temperature protection switch is opened and closed according to the monitoring and detection results of the temperature sensor to control the heating temperature of the heating element.

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