Capillary electrophoresis analyzer for nucleic acid fragments

Abstract

The utility model provides a nucleic acid fragment capillary electrophoresis analyzer which comprises a detection instrument main body, and a detection assembly is arranged on the surface of a capillary body. Two electrodes of a high-voltage power supply are respectively inserted into the two buffer solution pools, the electrophoresis in the capillary tube body is driven to move through the high-voltage power supply box, and the capillary electrophoresis adopts an on-column detection method to remove an opaque polyimide coating protective coating at the position, close to a port, of the capillary tube body due to the fact that the inner diameter of the capillary tube is extremely small; the fixing base and the pressing piece are attached to the surface of the capillary tube body through the fixing bolts, the light transmitting opening is aligned with a light path, the ultraviolet visible light detector conducts on-column detection on the capillary tube body through the spherical lens and the light transmitting opening, the photometric value is measured in cooperation with the photosensitive element, and therefore electrophoresis in the capillary tube body is analyzed. And each component is dynamically and visually recorded in an absorption peak form on a screen of the detection instrument main body by taking the migration time as a horizontal coordinate and the absorbance as a vertical coordinate, so that the accuracy is high.

Description

Technical Field

[0001] The utility model relates to the technical field of electrophoresis analyzer, in particular to a nucleic acid fragment capillary electrophoresis analyzer. Background Art

[0002] An electrophoresis analyzer refers to an instrument that utilizes the phenomenon that charged particles move towards electrodes with opposite polarities under the action of an electric field to achieve the separation and analysis of multi-component substances. Generally, substance molecules are not charged under normal circumstances, that is, the positive and negative charges carried are equal, and they do not show electrification. However, under certain physical actions or chemical reaction conditions, some substance molecules will become charged particles. By using the property that charged particles have different moving speeds and directions due to different charged properties, particle shapes, sizes, and charges carried, the separation and analysis of different components in a sample are achieved;

[0003] An electrophoresis instrument provided by the publication number "CN211505327U" relates to the technical field of electrophoresis instruments. It includes an electrophoresis instrument body, a box door is connected to the top of the electrophoresis instrument body through a hinge, an insulating handle is fixedly connected to the upper right side of the box door, a control panel is fixedly connected to the left side of the front surface of the electrophoresis instrument body, a working slot is opened on the left side of the inner cavity of the electrophoresis instrument body, an electrophoresis tank is fixedly connected to the bottom of the inner cavity of the working slot, a temperature detector is fixedly connected to the upper left side of the inner cavity of the working slot, a placement slot is fixedly connected to the right side of the inner cavity of the electrophoresis instrument body, a heat dissipation fan is fixedly connected to the right side of the driving rotating shaft, and a connection hole is opened at the lower end of the rear surface of the electrophoresis instrument body. It can well monitor the temperature of the inner cavity of the body in real time through the set temperature detector, dissipate heat by controlling the driving motor through a wire on one side of the control panel, and the driving motor drives the heat dissipation fan to dissipate heat through the driving rotating shaft, and gas transmission is carried out through a gas input pipe;

[0004] However, the existing filtration device still has the following defects:

[0005] Although the above electrophoresis instrument can well monitor the temperature of the inner cavity of the body in real time through the set temperature detector and facilitate heat dissipation, the accuracy of the electrophoresis instrument is poor due to the influence of external factors. Summary of the Utility Model

[0006] The purpose of the utility model is to provide a nucleic acid fragment capillary electrophoresis analyzer to solve the problems raised in the above background art.

[0007] To achieve the above purpose, the utility model provides the following technical solutions:

[0008] A nucleic acid fragment capillary electrophoresis analyzer, comprising a detection instrument main body, wherein a high-voltage power supply box is arranged inside the detection instrument main body, wires are inserted at both ends of the electrodes of the high-voltage power supply box, and platinum wire heads are installed at the ends of the two wires away from the high-voltage power supply box. Buffer solution pools are arranged on both sides of the high-voltage power supply box inside the detection instrument main body. A sample solution pool is arranged on one side of one of the buffer solution pools. The sample solution pool at a higher position enters the buffer solution pool through the siphon effect. A capillary body is arranged inside the detection instrument main body, and both ends of the capillary body are respectively inserted into the two buffer solution pools. An ultraviolet-visible light detector is arranged inside the detection instrument main body. It is characterized in that: a detection component is arranged on the surface of the capillary body;

[0009] The detection component includes a fixed base, a pressing piece, a fixing bolt, a capillary guide groove, a light-transmitting port, a photosensitive element and a spherical lens. A fixed base is arranged on one side of the surface of the capillary body, and a pressing piece is arranged on the other side of the capillary body. Capillary guide grooves are formed on the surfaces of the pressing piece and the fixed base. Fixing bolts are inserted into the jacks formed at the four corner positions on the surface of the pressing piece. The fixing bolts penetrate through the pressing piece and are threadedly connected to the threaded holes formed on the surface of the fixed base. A light-transmitting port is formed on the surface of the fixed base. A photosensitive element is arranged on one side surface of the fixed base at a position on one side of the light-transmitting port. A slit sheet is arranged between the fixed base and the pressing piece and the capillary body. A light-collecting hole is also formed on the surface of the pressing piece. A spherical lens is arranged at the position of the light-collecting hole above the pressing piece.

[0010] Preferably, one end of the capillary body is arranged in a spiral structure, and the capillary body is made of fused quartz material.

[0011] Preferably, a polyimide coating is installed on the surface of the capillary body, and a stationary phase layer is arranged on the inner wall of the capillary body.

[0012] Preferably, the high-voltage power supply box is a DC power supply with 0-30 kV, stable output, continuously adjustable, and the power supply polarity is easy to reverse.

[0013] Preferably, a screw sleeve is installed at the position of the light-collecting hole formed on the surface of the pressing piece. A focusing tube is threadedly connected inside the screw sleeve. The spherical lens is installed inside the focusing tube, and a light-collecting tube is installed at one end of the focusing tube.

[0014] Preferably, the light-collecting tube is arranged in a conical structure, and a tin-nickel electroplated coating is applied on the inner wall surface of the light-collecting tube.

[0015] Compared with the prior art, the beneficial effects of the present utility model are as follows: The two electrodes of the high-voltage power supply are respectively inserted into two buffer solution pools, and the capillary electrophoresis in the capillary body is driven by the high-voltage power supply box. Since the inner diameter of the capillary is extremely small, the capillary electrophoresis adopts the method of on-column detection. At a position near the port of the capillary body, the opaque polyimide coating protection coating is removed, and the fixed base and the pressing piece are attached to the surface of the capillary body through the fixing bolts. By aligning the light-transmitting port with the optical path, the ultraviolet-visible light detector passes through the spherical lens and passes through the light-transmitting port to perform on-column detection on the capillary body, and cooperates with the photosensitive element to measure the photometric value, so as to realize the analysis of the electrophoresis in the capillary body, and on the screen of the detection instrument main body, with the migration time as the abscissa and the absorbance as the ordinate, each component is dynamically and intuitively recorded in the form of absorption peaks, with high accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Schematic structural diagram of the detection component in the present utility model;

[0017] Figure 2 Schematic overall structure diagram of the present utility model;

[0018] Figure 3 Cross-sectional view of the capillary body in the present utility model;

[0019] Figure 4 Schematic internal structure diagram of the focusing tube in the present utility model.

[0020] In the figure: 1. Detection instrument main body; 2. High-voltage power supply box; 3. Wire; 4. Buffer solution pool; 5. Sample solution pool; 6. Capillary body; 7. Detection mechanism; 8. Stationary phase layer; 9. Polyimide coating; 10. Fixed base; 11. Pressing piece; 12. Fixing bolt; 13. Capillary guide groove; 14. Light-transmitting port; 15. Photosensitive element; 16. Screw sleeve; 17. Light-collecting tube; 18. Focusing tube; 19. Spherical lens. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0022] Please refer to Figures 1-4 , the present utility model provides a technical solution: Embodiment 1:

[0023] A nucleic acid fragment capillary electrophoresis analyzer, comprising a detection instrument main body 1. Inside the detection instrument main body 1, there is a high-voltage power supply box 2. Both ends of the electrodes of the high-voltage power supply box 2 are plugged with wires 3. One end of each of the two wires 3 away from the high-voltage power supply box 2 is equipped with a platinum wire head. On both sides of the high-voltage power supply box 2 inside the detection instrument main body 1, there are buffer solution pools 4. On one side of one of the buffer solution pools 4, there is a sample solution pool 5. The sample solution pool 5 at a higher position enters the buffer solution pool 4 through the siphon effect. Inside the detection instrument main body 1, there is a capillary body 6. Both ends of the capillary body 6 are respectively inserted into the two buffer solution pools 4. Inside the detection instrument main body 1, there is an ultraviolet-visible light detector, and a detection component is arranged on the surface of the capillary body 6;

[0024] The detection component includes a fixed base 10, a pressing piece 11, fixing bolts 12, a capillary guide groove 13, a light-transmitting port 14, a photosensitive element 15, and a spherical lens 19. On one side of the surface of the capillary body 6, there is a fixed base 10. On the other side of the capillary body 6, there is a pressing piece 11. Capillary guide grooves 13 are provided on the surfaces of both the pressing piece 11 and the fixed base 10. In the insertion holes opened at the four corner positions on the surface of the pressing piece 11, fixing bolts 12 are respectively plugged. The fixing bolts 12 penetrate through the pressing piece 11 and are threadedly connected to the threaded holes opened on the surface of the fixed base 10. A light-transmitting port 14 is opened on the surface of the fixed base 10. On one side surface of the fixed base 10 at a position on one side of the light-transmitting port 14, there is a photosensitive element 15. A slit plate is arranged between the fixed base 10 and the pressing piece 11 and the capillary body 6. A light-gathering hole is also opened on the surface of the pressing piece 11. Above the pressing piece 11 at the position of the light-gathering hole, there is a spherical lens 19. For the ultraviolet-visible light detector, due to the extremely small inner diameter of the capillary, column-on detection method is adopted in capillary electrophoresis. At the position near the port of the capillary body 6, the opaque polyimide coating 9 protection coating is removed. The fixed base 10 and the pressing piece 11 are attached to the surface of the capillary body 6 through the fixing bolts 12. By aligning the light-transmitting port 14 with the light path, the ultraviolet-visible light detector passes through the spherical lens 19 and passes through the light-transmitting port 14 to perform column-on detection on the capillary body 6, and cooperate with the photosensitive element 15 to measure the photometric value, so as to realize the analysis of the electrophoresis in the capillary body 6, and on the screen of the detection instrument main body 1, with the migration time as the abscissa and the absorbance as the ordinate, each component is dynamically and intuitively recorded in the form of absorption peaks;

[0025] One end of the capillary body 6 is arranged in a spiral structure. The capillary body 6 is made of fused quartz material. Since the column-on detection method is adopted for the capillary body 6, it is necessary to ensure that the capillary body 6 is permeable to ultraviolet and visible light for convenient column-on detection and improve the detection accuracy;

[0026] A polyimide coating 9 is installed on the surface of the capillary body 6, and a stationary phase layer 8 is arranged on the inner wall of the capillary body 6, which can improve the photosensitivity of the capillary body 6;

[0027] The high-voltage power supply box 2 provides a DC power supply with an output range of 0 - 30 kV, which is stable and continuously adjustable, and the power supply polarity can be easily reversed. The high-voltage power supply is the driving force for the capillary body 6. The daily power supply is 220V AC power, which cannot be used for electrophoresis separation. Otherwise, the direction of the force on the charged particles in the AC electric field will change periodically, and no directional migration will occur. Example Two:

[0028] Although the above technical solution can achieve on-column detection through the screening component and improve the accuracy of electrophoresis separation detection, a screw sleeve 16 is installed at the position of the light-collecting hole on the surface of the pressing piece 11. The focusing tube 18 is connected to the screw sleeve 16 by internal threads. The spherical lens 19 is installed in the focusing tube 18, and a light-collecting tube 17 is installed at one end of the focusing tube 18. By installing the screw sleeve 16 at the position of the light-collecting hole on the surface of the pressing piece 11, the focusing tube 18 with the spherical lens 19 installed is rotated in the screw sleeve 16. By rotating the focusing tube 18, the use position of the spherical lens 19 can be adjusted, making it more flexible and convenient to use.

[0029] The light-collecting tube 17 is arranged in a conical structure, and a tin-nickel electroplated coating is applied to the inner wall surface of the light-collecting tube 17, which can greatly improve the transmission efficiency of the ultraviolet-visible light detector.

[0030] Working principle: Two electrodes of the high-voltage power supply box 2 are respectively inserted into two buffer solution pools 4. The high-voltage power supply box 2 drives the electrophoresis movement in the capillary body 6. Due to the extremely small inner diameter of the capillary, on-column detection is adopted in capillary electrophoresis. At the position near the port of the capillary body 6, the opaque polyimide coating 9 protection coating is removed. The fixing base 10 and the pressing piece 11 are attached to the surface of the capillary body 6 through the fixing bolts 12. By aligning the light-transmitting port 14 with the light path, the ultraviolet-visible light detector passes through the spherical lens 19 and passes through the light-transmitting port 14 to perform on-column detection on the capillary body 6, and cooperates with the photosensitive element 15 to measure the photometric value, so as to realize the analysis of the electrophoresis in the capillary body 6, and on the screen of the detection instrument main body 1, with the migration time as the abscissa and the absorbance as the ordinate, each component is dynamically and intuitively recorded in the form of absorption peaks. The focusing tube 18 with the spherical lens 19 installed is rotated in the screw sleeve 16. By rotating the focusing tube 18, the use position of the spherical lens 19 can be adjusted, making it more flexible and convenient to use.

[0031] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A nucleic acid fragment capillary electrophoresis analyzer, comprising a detection instrument body (1), wherein a high-voltage power supply box (2) is arranged inside the detection instrument body (1), and both ends of the electrodes of the high-voltage power supply box (2) are plugged with wires (3), and the ends of the two wires (3) away from the high-voltage power supply box (2) are installed with platinum wire heads, and buffer solution pools (4) are arranged on both sides of the high-voltage power supply box (2) in the detection instrument body (1), and a sample solution pool (5) is arranged on one side of one of the buffer solution pools (4), and the sample solution pool (5) is arranged at a higher position and enters the buffer solution pool (4) through a siphon effect, and a capillary body (6) is arranged in the detection instrument body (1), and the two ends of the capillary body (6) are respectively inserted into the two buffer solution pools (4), characterized in that: A detection component is provided on the surface of the capillary body (6); The detection assembly comprises a fixed base (10), a pressing plate (11), a fixing bolt (12), a capillary guide groove (13), a light transmission port (14), a photosensitive element (15) and a ball lens (19); the fixed base (10) is arranged on one side of the surface of the capillary body (6); the pressing plate (11) is arranged on the other side of the capillary body (6); the surfaces of the pressing plate (11) and the fixed base (10) are both provided with capillary guide grooves (13); the fixing bolts (12) are inserted into the sockets at the four corners of the surface of the pressing plate (11); A fixing bolt (12) passes through the pressing plate (11) and is threadedly connected to a threaded hole provided on the surface of the fixing base (10); a light-transmitting opening (14) is provided on the surface of the fixing base (10); a photosensitive element (15) is provided on one side of the surface of the fixing base (10) at a position on one side of the light-transmitting opening (14); a slit is provided between the fixing base (10), the pressing plate (11) and the capillary body (6); a light-collecting hole is also provided on the surface of the pressing plate (11); and a ball lens (19) is provided above the pressing plate (11) at the position of the light-collecting hole.

2. A nucleic acid fragment capillary electrophoresis analyzer according to claim 1, characterized in that: One end of the capillary body (6) is arranged in a spiral structure, and the capillary body (6) is made of fused quartz material.

3. A nucleic acid fragment capillary electrophoresis analyzer according to claim 1, characterized in that: A polyimide coating (9) is installed on the surface of the capillary body (6), and a stationary phase layer (8) is provided on the inner wall of the capillary body (6).

4. A nucleic acid fragment capillary electrophoresis analyzer according to claim 1, characterized in that: The high voltage power supply box (2) is 0-30 kV, outputs a stable, continuously adjustable direct current power supply, and the polarity of the power supply is easily reversible.

5. A nucleic acid fragment capillary electrophoresis analyzer according to claim 1, characterized in that: A screw sleeve (16) is installed at the position of the light-collecting hole opened on the surface of the pressing plate (11); a focusing tube (18) is connected to the inner thread of the screw sleeve (16); the ball lens (19) is installed in the focusing tube (18); and a light-collecting tube (17) is installed at one end of the focusing tube (18).

6. A nucleic acid fragment capillary electrophoresis analyzer according to claim 5, characterized in that: The light tube (17) is arranged in a conical structure, and the inner wall surface of the light tube (17) is coated with a tin-nickel electroplating coating.

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