Empty column filling device for manually filling chromatographic column

Abstract

The filling device for manually filling the empty chromatographic column comprises a hollow pipe and at least two cylinders, the hollow pipe is arranged in the middles of the two cylinders in a penetrating mode, the two cylinders are arranged at intervals, and the upper portions of the cylinders are fixed to the inner wall of the chromatographic column through pull ropes. According to the empty column filling device for manually filling the chromatographic column, the at least two cylinders are arranged in the middle of the chromatographic column, and the two cylinders are spaced and adjacent through the hollow pipe, so that the two cylinders can relatively move in the chromatographic column, and the hollow column filling device is similar to a piston and can be used for drainage, and the problem of pounding of filler at the bottom of the chromatographic column is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of industrial chromatography, and more specifically to a manual empty column filling device for filling chromatography columns. Background Art

[0002] There are two ways to obtain protein purification chromatography columns: one is to purchase pre-packed columns of different specifications from the manufacturer, and the other is to manually pack them. Pre-packed columns cannot be disassembled, while manual packing allows for changing the type of packing material and adjusting the packing height, as well as convenient cleaning and multiple use.

[0003] The linear scale-up principle is the most commonly used principle in process scale-up. Its core idea is to keep the column height and linear flow rate unchanged, only scale up the column diameter and volume flow rate, and do not change the packing height.

[0004] When manually filling a small test to expand, sometimes it is not possible to purchase a proportionally enlarged column in time, and try to use other diameters on site that are suitable for the height but may not be suitable for column modification. Manually fill the filler to the appropriate height, screw down the column head, lock it and start using it.

[0005] Sometimes, even when the column screw is tightened all the way to the bottom, the column head is still too high. After packing, there is air inside the column. When you try to load the sample directly, even though liquid flows out from the bottom of the column, the liquid level above the packing rises. This compresses the air, increases the pressure inside the column, and creates pits in the packing.

[0006] Therefore, it is necessary to propose a manual empty column filling device to avoid the safety hazard of filler leakage caused by excessive gas accumulation in low-pressure and medium-pressure chromatography columns. At the same time, it is used for drainage to solve the problem that the filler is not flat due to the droplets being accelerated by gravity and causing pits to be smashed into the filler. Utility Model Content

[0007] The utility model provides a manual empty column filling device for filling chromatography columns, which solves the problem that liquid droplets are not accelerated by gravity during manual filling, resulting in pits in the filler and uneven filler.

[0008] According to one aspect of the present invention, a manual empty column filling device for filling a chromatography column is provided, comprising a hollow tube and at least two cylinders, wherein the hollow tube is passed through the middle of the two cylinders, the two cylinders are spaced apart, and the upper part of the cylinder is fixed to the inner wall of the chromatography column by a pull rope.

[0009] Preferably, based on the above solution, a feed cone is provided at the top of the hollow tube.

[0010] Preferably, based on the above solution, a chromatography column head is provided at the top of the chromatography column, and a filler is filled at the bottom of the chromatography column.

[0011] Preferably, based on the above solution, a sample loading hole is provided on the chromatography column head, and the top of the hollow tube is located below the sample loading hole.

[0012] Preferably, based on the above solution, the hollow tube is configured to have a conical head on a side close to the filler.

[0013] Preferably, based on the above solution, a suction cup is provided on the inner side of the chromatography column, and the pull rope is connected to the suction cup.

[0014] The utility model provides a manual empty column filling device for filling a chromatography column. At least two cylinders are arranged in the middle of the chromatography column. The two cylinders are separated and adjacent by a hollow tube so that they can move relatively in the chromatography column. Similar to a piston, they can not only be used for drainage, but also prevent the filler at the bottom of the chromatography column from being pitted. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative work. In the drawings:

[0016] Figure 1 This is a schematic diagram of the principle structure of the aerator of the utility model;

[0017] Description of Figure Numbers:

[0018] Hollow tube 1, cylinder 2, pull rope 3, chromatography column 4, feed cone 5, chromatography column head 6, filler 7, sample loading hole 8, suction cup 9. DETAILED DESCRIPTION

[0019] The following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0020] It will be understood that when used in this specification and the appended claims, the term "comprising" indicates the presence of described features, integers, steps, operations, elements and / or components, but does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or collections.

[0021] To simplify the drawings, only the parts relevant to the present invention are schematically shown in each figure. They do not represent the actual structure of the product. In addition, to simplify the drawings and facilitate understanding, in some figures, only one of the components with the same structure or function is schematically depicted or labeled. In this document, "one" not only means "only one" but also "more than one."

[0022] It should be further understood that the term "and / or" used in this specification and the appended claims refers to and includes any and all possible combinations of one or more of the associated listed items.

[0023] In the embodiments shown in the accompanying drawings, directional indications (such as up, down, left, right, front, and back) used to explain the structure and movement of various components of the present invention are not absolute but relative. These descriptions are applicable when these components are in the positions shown in the accompanying drawings. If the descriptions of the positions of these components are changed, these directional indications will also change accordingly.

[0024] In addition, in the description of the present application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the specific implementation methods of the present invention will be described below with reference to the accompanying drawings. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings and other implementation methods can be obtained based on these drawings without inventive work.

[0026] See also Figure 1 As shown, a manual filling chromatography column empty column filling device of the present invention includes a hollow tube 1 and at least two cylinders 2. The hollow tube 1 is passed through the middle of the two cylinders 2. The two cylinders 2 are arranged at intervals, and the upper part of the cylinder 2 is fixed to the inner wall of the chromatography column 4 by a pull rope 3.

[0027] Specifically, a feed cone 5 is provided at the top of the hollow tube 1 to facilitate feeding, and a chromatography column head 6 is provided at the top of the chromatography column 4. The bottom of the chromatography column 4 is filled with filler 7. A loading hole 8 is provided on the chromatography column head 6. The top of the hollow tube 1 is located below the loading hole 8, so that the feed through the loading hole 8 can be accurately introduced into the hollow tube 1.

[0028] In order to reduce the effect of the liquid on the chromatography filler 7, the utility model provides a conical head on the side of the hollow tube 1 close to the filler 7.

[0029] As a preferred embodiment of the present invention, a suction cup 9 is provided on the inner side of the chromatography column 4 of the present invention, and the pull rope 3 is connected to the suction cup 9. By adjusting the length of the pull rope 3, the relative position of the cylinder 2 in the chromatography column 4 can be adjusted.

[0030] The utility model is applicable to the situation that the sum of the height of the chromatography column head 6 and the height of the filler 7 is smaller than the total height of the chromatography column 4 .

[0031] The following specific embodiments are used to illustrate the environment in which the present invention is used; when the diameter of the stainless steel chromatography column 4 is 20 cm, and its height is 50 cm, the chromatography column head 6 is 70 cm high.

[0032] Since the chromatography column head 6 is higher than the chromatography column 4, there is no air section and any height of the filler 7 can be used. Therefore, the structure of the present utility model can be omitted.

[0033] When the diameter of the stainless steel chromatography column 4 is 30 cm and its height is 195 cm, the chromatography column head 6 is 70 cm high, the filler is 725 cm, and the air section is 100 cm long.

[0034] The stainless steel chromatography column 4 suffers from a short column head 6. The present invention uses a 3 cm diameter suction cup 9 and a 10 cm tether, attached to the upper cylinder 2. The two cylinders 2 have a diameter of 298 mm. A hollow tube 1 made of hard plastic in the middle is integrated with the two cylinders 2. The stainless steel chromatography column 4 has an outer diameter of 6 mm, an inner diameter of 3 mm, and a total length of 100 cm. The top receiving port is slightly larger, with a diameter of 2 cm, and the bottom outlet is slightly pointed, with an outer diameter of 4 mm and an inner diameter of 2 mm.

[0035] After using the drainage method of the utility model, the original liquid distribution plate (1 cm thick) above the column head does not work. As long as the liquid height on the filler 7 is controlled low, within 1 cm, it is observed that the colored fermentation liquid can diffuse and distribute the sample by itself without affecting the column efficiency.

[0036] The utility model provides a manual filling device for an empty chromatography column, which arranges at least two cylinders 2 in the middle of the chromatography column and separates the two cylinders 2 adjacent to each other through a hollow tube 1 so that they can move relatively in the chromatography column, similar to a piston. It can not only be used for drainage, but also prevent the filler 7 at the bottom of the chromatography column from being dented.

[0037] Finally, the method of this application is only a preferred embodiment and is not intended to limit the scope of protection of this utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of this utility model should be included in the scope of protection of this utility model.

Claims

1. A manual empty column filling device for filling chromatography columns, characterized in that: It comprises a hollow tube and at least two cylinders, wherein the hollow tube is passed through the middle of the two cylinders, the two cylinders are arranged at intervals, and the upper part of the cylinder is fixed to the inner wall of the chromatography column by a drawstring.

2. A manually loaded chromatography column empty column filling device as claimed in claim 1, characterized in that: A feeding cone is arranged on the top of the hollow tube.

3. A manually loaded chromatography column empty column filling device as claimed in claim 1, characterized in that: The top of the chromatography column is provided with a chromatography column head, and the bottom of the chromatography column is filled with fillers.

4. A manually loaded chromatography column empty column filling device as claimed in claim 3, characterized in that: The chromatography column head is provided with a loading hole, and the top of the hollow tube is located below the loading hole.

5. A manually loaded chromatography column empty column filling device as claimed in claim 3, characterized in that: The hollow tube is arranged to have a conical head on one side close to the filler.

6. A manually loaded chromatography column empty column filling device as claimed in claim 1, characterized in that: A suction cup is arranged on the inner side of the chromatography column, and the pull rope is connected to the suction cup.

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