Microinjection roe fixing mold

Abstract

The utility model discloses a micro-injection roe fixing mold, which relates to the technical field of biological experiment molds and comprises a bottom plate. The plurality of comb-shaped toothed bars are symmetrically and fixedly arranged on the upper wall of the bottom plate; and the identification mark is fixedly arranged on the bottom plate. The utility model is suitable for preparing the roe fixing tool by using the agarose gel. Due to the fact that the formed groove is of an inclined triangular conical structure, the resultant force of the acting force (Fa, Fb and Fc) of the three faces a, b and c and the gravity G on the fertilized eggs is zero, a certain angle is formed between an injection needle and the eggs during micro-injection, and the resultant force borne by the fertilized eggs cannot be changed by the force (Fi) of the injection needle. Besides, the friction force is greatly increased through the three stress surfaces, Fa, Fb and Fc are increased along with increase of injection force Fi, fertilized eggs cannot move and are not prone to slipping during micro-injection, and the device is particularly suitable for fish eggs which are high in hardness, high in elasticity and difficult to inject. And the device is suitable for roes with different sizes.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experiment molds, in particular to a microinjection fish egg fixing mold. Background Art

[0002] There are many kinds of cultured fish in China. Cultivating high-quality aquatic product varieties is one of the core tasks of the industrial development. However, the traditional artificial selection has a long cycle, low efficiency and high cost. As one of the most cutting-edge technologies in the current field of life science, gene editing technology shows broad application prospects in many fields such as biological gene function research, animal and plant disease prevention and control, and variety improvement. Microinjection is the most commonly used means for delivering gene editing substances in animals.

[0003] For most fish, at room temperature, its incubation period is generally 2 - 7 days, and the fertilized egg generally undergoes cell division about 1 hour after fertilization. In order to improve the efficiency of gene editing, microinjection is generally performed at the 1-cell stage before cell division. Therefore, the window period for microinjection is very short, and how to improve the injection efficiency is very crucial.

[0004] Currently, zebrafish is the most commonly used fish in gene editing technology. Since its fertilized eggs absorb water and expand, and the egg membrane is relatively thin and easy to inject, the commonly used egg fixing tool is an agarose gel groove, which is made by a simple cuboid mold. Its characteristics are simple and convenient. However, due to the non-uniform size of fish eggs and the continuous expansion of fertilized eggs by absorbing water, a cuboid groove of one size cannot effectively fix all types of fish eggs, and it cannot effectively fix fish eggs with high hardness and strong elasticity. In addition, as the fish eggs develop, the egg membrane will gradually harden. If the fish eggs cannot be effectively fixed, microinjection cannot be successfully completed. Therefore, it is necessary to improve the cuboid mold in the prior art. Content of the Utility Model

[0005] The purpose of the utility model is to provide a microinjection fish egg fixing mold to solve the problems mentioned in the above background art.

[0006] To solve the above problems, the technical solutions adopted by the utility model are as follows:

[0007] A microinjection fish egg fixing mold includes

[0008] a bottom plate;

[0009] comb-shaped tooth rods, and a plurality of the comb-shaped tooth rods are symmetrically and fixedly arranged on the upper wall of the bottom plate;

[0010] an identification mark, and the identification mark is fixedly arranged on the bottom plate.

[0011] Preferably, the comb-shaped tooth bar is composed of a first structure body and a second structure body. The first structure body is formed by obliquely truncating a cuboid at the distal end, and the second structure body is formed by obliquely truncating a triangular prism at the distal end. Moreover, the truncated sections of the first structure body and the second structure body form continuous sections with the same inclination angle.

[0012] Preferably, a plurality of second structure bodies are fixedly arranged on one side wall of the first structure body.

[0013] Preferably, the identification mark is in the shape of a cross.

[0014] Preferably, the identification mark is in the shape of a straight line.

[0015] Preferably, two through holes are symmetrically formed in the upper wall of the bottom plate.

[0016] Preferably, the bottom plate, the comb-shaped tooth bar, and the identification mark are all made of lightweight and high-temperature-resistant materials.

[0017] Compared with the prior art, the utility model has the following beneficial effects:

[0018] The utility model is applicable to the preparation of fish egg fixing tools using agarose gel. Since the formed groove is in an inclined triangular pyramid structure, the fertilized eggs are subjected to the acting forces (Fa, Fb, Fc) of three surfaces a, b, and c, plus the gravity G, and their resultant force is zero. During microinjection, when the injection needle forms a certain angle with the egg, the applied force (Fi) will not change the resultant force on the fertilized eggs. In addition, the three force-bearing surfaces greatly increase the friction force, and as the injection force Fi increases, Fa, Fb, and Fc will also increase accordingly, making the fertilized eggs not move and not slip easily during microinjection, especially suitable for fish eggs with high hardness, strong elasticity, and difficult injection. And due to the special design of the triangular pyramid, it can be applicable to fertilized eggs of different sizes. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall external structure of a microinjection fish egg fixing mold;

[0020] Figure 2 It is a schematic diagram of the structure of a microinjection fish egg fixing mold from the first perspective;

[0021] Figure 3 It is a schematic diagram of the comb-shaped tooth bar structure of a microinjection fish egg fixing mold;

[0022] Figure 4 It is a schematic diagram of the force on the fish egg during injection of a microinjection fish egg fixing mold.

[0023] In the figure: 1. Bottom plate; 2. Through hole; 3. Identification mark; 4. First structure body; 5. Second structure body. Detailed implementation manners

[0024] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0025] Embodiment 1:

[0026] Please refer to Figures 1-4 As shown, the present invention is a microscopic injection fish egg fixing mold, including a bottom plate 1;

[0027] Comb-shaped tooth rods, a plurality of comb-shaped tooth rods are symmetrically and fixedly arranged on the upper wall of the bottom plate 1;

[0028] Identification mark 3, the identification mark 3 is fixedly arranged on the bottom plate 1.

[0029] It can be seen from Figures 1-3 that the comb-shaped tooth rod is composed of a first structure body 4 and a second structure body 5. The first structure body 4 is formed by making an oblique cut at the distal end of a cuboid, and the second structure body 5 is formed by making an oblique cut at the distal end of a triangular prism, and the cuts of the first structure body 4 and the second structure body 5 form a continuous cross-section with the same inclination angle.

[0030] A plurality of second structure bodies 5 are fixedly arranged on one side wall of the first structure body 4.

[0031] The identification mark 3 is in a cross shape.

[0032] The identification mark 3 is in a linear shape.

[0033] The bottom plate 1, the comb-shaped tooth rod and the identification mark 3 are all made of a light and high-temperature resistant material;

[0034] Specifically, the material of this mold is preferably a material such as plastic or nylon that is lighter and not easily deformed at high temperatures. During the manufacturing process, 3D printing or corresponding molds can be used for preparation.

[0035] As can be seen from the above, the mold of the present invention is mainly used to prepare an agarose gel model in a culture dish of 6 cm, 10 cm or other sizes. Among them, the specific preparation method of the agarose gel model is as follows:

[0036] First, prepare a 1.5% agarose solution, pour the boiled and mixed agarose solution into the culture dish, then invert the mold of the present invention on the surface of the agarose solution. After the agarose solution solidifies, take out this mold to obtain a fish egg fixing tool for microscopic injection.

[0037] When performing microinjection on fish eggs, pour a liquid such as water or embryo culture medium into a petri dish and completely immerse the agarose gel. Then place the fertilized fish eggs in the groove. For sinking eggs, they will fall into the groove along the slope by themselves and be fixed.

[0038] See Figure 4 As can be seen, since the formed groove has an inclined triangular pyramid structure, the fertilized eggs are subjected to the acting forces (Fa, Fb, Fc) of the three surfaces a, b, and c. Coupled with the gravity G, the resultant force is zero. During microinjection, the injection needle forms a certain angle with the egg, and the force applied (Fi) will not change the resultant force received by the fertilized eggs. In addition, the three force-receiving surfaces greatly increase the friction force. Moreover, as the injection force Fi increases, Fa, Fb, and Fc will also increase accordingly, making the fertilized eggs not move and not easily slip during microinjection. It is especially suitable for fish eggs with high hardness, strong elasticity, and difficult injection. And due to the special design of the triangular pyramid, it can be applied to fertilized eggs of different sizes.

[0039] Example Two:

[0040] Bottom plate 1;

[0041] Comb-shaped tooth rods, several comb-shaped tooth rods are symmetrically and fixedly arranged on the upper wall of the bottom plate 1;

[0042] Identification mark 3, the identification mark 3 is fixedly arranged on the bottom plate 1.

[0043] Reference Figure 1 And Figure 2 As shown, two through holes 2 are symmetrically formed on the upper wall of the bottom plate 1.

[0044] As can be seen from the above, in Example One, after the agarose solution solidifies, the staff can fix the mold of the present invention through the through holes 2 with the help of tools such as tweezers, and then it is convenient to further take out this mold. The structural design is user-friendly.

[0045] In the present invention, unless otherwise clearly specified and limited, terms such as "installation", "connection", "connection", "fixation" and other terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be directly connected, or indirectly connected through an intermediate medium. It can be the internal communication of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0046] In the present utility model, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature may be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may be that the first feature is directly above or obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "underneath" the second feature may be that the first feature is directly below or obliquely below the second feature, or merely indicates that the horizontal height of the first feature is less than that of the second feature.

[0047] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representations of the above terms do not have to be directed to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0048] In the attached drawings of the disclosed embodiments of the present utility model, only the structures related to the disclosed embodiments are involved, and other structures can refer to the general design. Without conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

[0049] Although the present utility model has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A microinjection fish egg fixing mold, characterized in that: include Bottom plate (1); Comb-shaped tooth rods, a plurality of the comb-shaped tooth rods are symmetrically and fixedly arranged on the upper wall of the bottom plate (1); An identification mark (3), wherein the identification mark (3) is fixedly arranged on the bottom plate (1).

2. A microinjection fish egg fixing mold according to claim 1, characterized in that: The comb-shaped tooth rod is composed of a first structure (4) and a second structure (5), wherein the first structure (4) is formed by a rectangular parallelepiped with an oblique cut at the far end, and the second structure (5) is formed by a triangular prism with an oblique cut at the far end, and the first structure (4) and the second structure (5) are cut to form a continuous cross section with a consistent inclination angle.

3. A microinjection fish egg fixing mold according to claim 2, characterized in that: A plurality of second structures (5) are fixedly provided on one side wall of the first structure (4).

4. The microinjection fish egg fixing mold according to claim 1, characterized in that: The identification mark (3) is in the shape of a cross.

5. The microinjection fish egg fixing mold according to claim 1, characterized in that: The identification mark (3) is in the shape of a letter.

6. The microinjection fish egg fixing mold according to claim 1, characterized in that: Two through holes (2) are symmetrically provided on the upper wall of the bottom plate (1).

7. The microinjection fish egg fixing mold according to claim 1, characterized in that: The base plate (1), the comb-shaped tooth rod and the identification mark (3) are all made of lightweight and high-temperature resistant materials.

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