Macaca fascicularis specific SNP site combination, liquid chip and application of macaca fascicularis specific SNP site combination

Abstract

The invention relates to the field of molecular genetics, in particular to a cynomolgus monkey specific SNP site combination, a liquid chip and application of the cynomolgus monkey specific SNP site combination and the liquid chip, 1508 cynomolgus monkey specific SNP sites disclosed and screened by the invention have high polymorphism, and rapid identification, paternity identification and lifelong tracking identification of cynomolgus monkey individuals can be realized. Liquid phase chip design is carried out on the sites, chip inspection is carried out on 383 cynomolgus monkey samples from three different groups, the result shows that the average site detection rate is 99.47%, and it is indicated that the liquid phase chip has excellent site capturing efficiency. According to the method, the genetic detection capability and accuracy of the cynomolgus monkeys can be greatly improved, the genetic detection cost can be remarkably reduced, and an important technical support is provided for improving the breeding management of the cynomolgus monkeys and optimizing the population structure.

Description

Technical Field

[0001] The present invention relates to the field of molecular genetics, and particularly to a combination of specific SNP sites of cynomolgus monkeys, a liquid-phase chip and their applications. Background Art

[0002] The cynomolgus monkey (Macaca fascicularis), also known as the long-tailed macaque or crab-eating macaque, has many physiological and anatomical characteristics similar to those of humans, such as the cardiovascular system, immune system, nervous system, etc. This makes it an important non-human primate experimental animal in addition to the rhesus monkey. Since it reproduces relatively fast, with an adult body weight of about 4 kg - 5 kg, which is smaller than the average 7 kg rhesus monkey, the dosage of medicine used is also correspondingly smaller. Therefore, since the 1970s, experimental monkeys have been fully switched to cynomolgus monkeys. The experimental monkeys bred in China are mainly cynomolgus monkeys, accounting for about 80% - 85% of the total industry output. Since cynomolgus monkeys are not native to China, breeding monkeys need to be introduced from Southeast Asia. However, in recent years, the wild cynomolgus monkey population in Southeast Asia has decreased sharply, and it is impossible to maintain the genetic diversity of the existing breeding population by relying on introduced breeding sources. Using effective genetic markers to identify the genetic background of cynomolgus monkeys, especially the confirmation of paternity, is particularly crucial. Through paternity testing, the genetic background of experimental animals can be ensured to be clear, and genetic problems caused by inbreeding can be avoided, which is very important for optimizing the population structure and maintaining the long-term healthy development of the cynomolgus monkey breeding industry.

[0003] The key to paternity testing lies in selecting appropriate genetic markers. In cynomolgus monkeys, the commonly used genetic markers are mainly microsatellite sequences. However, the information contained in the currently used microsatellite markers is limited, and the experimental operation steps are cumbersome, and the cost is relatively high, which cannot meet the needs of large-scale sample paternity testing. As a form of genetic variation, single nucleotide polymorphism (SNP) has become the preferred marker for genetic analysis in recent years due to its rich polymorphism, high genetic stability, wide distribution and easy detection. However, so far, the application potential of these SNP site information in the individual identification and paternity testing of cynomolgus monkeys has not been fully explored.

[0004] In the technology of genotyping SNP loci, the liquid chip technology based on Genotyping By Target Sequencing (GBTS) has shown great application potential in multiple fields in recent years. This technology designs specific probes for each target SNP locus and specifically captures all target loci in the genome in the same liquid reaction system. Through processes such as magnetic bead adsorption, elution, amplification, library construction, and deep sequencing, accurate detection of target loci can be achieved. At the same time, this high-throughput characteristic gives the liquid chip technology significant advantages in processing a large number of samples and can effectively reduce the detection cost. This technology has complete independent intellectual property rights, is not restricted by foreign patent technologies, and does not rely on foreign equipment. So far, it has been widely used in species genetic detection and germplasm resource evaluation of livestock and poultry and aquatic animals such as rice, corn, pigs, and chickens. Therefore, by screening highly polymorphic SNP loci in the cynomolgus macaque genome and developing a set of liquid chip systems based on them, it will provide a powerful tool for individual identification, paternity testing, and evaluation of genetic diversity of cynomolgus macaques. Summary of the Invention

[0005] In order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a specific SNP locus combination for cynomolgus macaques, a liquid chip, and their applications.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] A specific SNP locus combination for cynomolgus macaques, which consists of 1508 polymorphic loci. The physical positions of the SNPs are determined based on the reference genome of a male T2T cynomolgus macaque assembled by this research group. The information (NO.1 - 1508) of the 1508 SNP loci is represented in the form of chromosome number_physical position_reference genotype / variant genotype:

[0008] NO.1: chr1_370185_G / A; NO.2: chr1_2443984_A / G; NO.3: chr1_5902345_G / C; NO.4: chr1_6685161_T / C; NO.5: chr1_8362268_G / A; NO.6: chr1_10462682_T / C; NO.7: chr1_12454979_G / C; NO.8: chr1_14734649_A / T; NO.9: chr1_16999706_T / A; NO.10: chr1_18682940_C / T; NO.11: chr1_22150881_T / C; NO.12: chr1_23934223_T / G; NO.13: chr1_25749025_C / T; NO.14: chr1_28068619_C / T; NO.15: chr1_33183373_G / A; NO.16: chr1_38139307_G / T; NO.17: chr1_39400436_G / A; NO.18: chr1_44264403_T / A; NO.19: chr1_46360543_G / A; NO.20: chr1_48282712_G / A; NO.21: chr1_51075911_A / C; NO.22: chr1_54323401_T / C; NO.23: chr1_56639355_C / T; NO.24: chr1_58184634_T / C; NO.25: chr1_61039870_G / A; NO.26: chr1_62216780_T / C; NO.27: chr1_64770950_T / C; NO.28: chr1_66455917_G / A; NO.29: chr1_68694365_C / T; NO.30: chr1_71886991_A / G; NO.31: chr1_72720826_A / G; NO.32: chr1_75407983_C / T; NO.33: chr1_76791240_G / A; NO.34: chr1_82618678_G / A; NO.35: chr1_83273989_C / G; NO.36: chr1_99735631_G / T; NO.37: chr1_101626621_A / G; NO.38: chr1_104157739_G / C; NO.39: chr1_108859582_G / T; NO.40: chr1_110298072_G / C; NO.41: chr1_112169980_C / T; NO.42: chr1_117131583_G / A; No. 43: chr1_118238765_A / T; No. 44: chr1_121470132_G / A; No. 45: chr1_124326609_A / T; No. 46: chr1_124798068_T / C; No. 47: chr1_126923007_G / A; No. 48: chr1_128682344_C / A; No. 49: chr1_130682615_G / A; No. 50: chr1_132986558_T / C; No. 51: chr1_135421231_A / G; No. 52: chr1_137065504_G / A; No. 53: chr1_140278193_C / T; No. 54: chr1_141877303_T / C; No. 55: chr1_143159693_G / A; No. 56: chr1_148171629_A / G; No. 57: chr1_149450631_G / A; No. 58: chr1_151930215_T / A; No. 59: chr1_154567904_G / A; No. 60: chr1_155891598_C / T; No. 61: chr1_157587583_G / A; No. 62: chr1_161897003_G / A; No. 63: chr1_164317304_G / A; No. 64: chr1_167077658_A / G; No. 65: chr1_169787993_A / C; No. 66: chr1_170546467_T / C; No. 67: chr1_174577152_C / G; No. 68: chr1_176765704_C / T; No. 69: chr1_178295330_T / C; No. 70: chr1_180545505_A / G; No. 71: chr1_182684165_C / T; No. 72: chr1_184479054_T / G; No. 73: chr1_187648265_G / A; No. 74: chr1_189808619_G / A; No. 75: chr1_190718794_C / T; No. 76: chr1_194035572_A / G; No. 77: chr1_195266681_A / C; No. 78: chr1_199013583_T / C; No. 79: chr1_201749758_A / T; No. 80: chr1_203159810_G / A; No. 81: chr1_205493758_T / C; No.82: chr1_207329249_G / A; No. 83: chr1_210818840_C / G; No. 84: chr1_211426749_G / A; No. 85: chr1_214500090_C / T; No. 86: chr1_217652131_T / G; No. 87: chr1_222024203_C / T; No. 88: chr1_225935080_T / C; No. 89: chr1_228124271_T / C; No. 90: chr1_230086149_G / C; No. 91: chr1_234252567_T / C; No. 92: chr10_753272_G / T; No. 93: chr10_3836973_C / A; No. 94: chr10_4165725_T / G; No. 95: chr10_6222088_T / C; No. 96: chr10_9089631_A / C; No. 97: chr10_11580131_C / T; No. 98: chr10_12905959_A / T; No. 99: chr10_14512685_A / G; No. 100: chr10_17907441_G / A; No. 101: chr10_18827091_C / T; No. 102: chr10_21933836_G / A; No. 103: chr10_24238619_T / G; No. 104: chr10_25367563_C / T; No. 105: chr10_26948155_A / G; No. 106: chr10_29090743_C / T; No. 107: chr10_32476526_T / C; No. 108: chr10_33553868_C / G; No. 109: chr10_35926605_C / G; No. 110: chr10_37355110_A / G; No. 111: chr10_41003236_T / C; No. 112: chr10_42095579_A / T; No. 113: chr10_44097896_G / A; No. 114: chr10_49969395_C / T; No. 115: chr10_54082734_T / A; No. 116: chr10_57953615_T / C; No. 117: chr10_63896238_T / C; No. 118: chr10_64551138_T / A; No. 119: chr10_77738289_A / C; No. 120: chr10_78970043_A / G; No.121: chr10_84791486_G / A; No. 122: chr10_85236155_G / C; No. 123: chr10_87126229_C / G; No. 124: chr10_89173181_G / A; No. 125: chr10_91657553_A / G; No. 126: chr10_95052459_T / G; No. 127: chr10_97274484_T / C; No. 128: chr10_97928174_A / G; No. 129: chr10_99674707_T / C; No. 130: chr10_103579176_C / T; No. 131: chr10_103655084_A / G; No. 132: chr10_106267636_G / A; No. 133: chr10_108986117_G / C; No. 134: chr10_109859527_G / A; No. 135: chr10_114368809_C / T; No. 136: chr11_1925570_A / G; No. 137: chr11_2390642_A / G; No. 138: chr11_4153795_T / C; No. 139: chr11_6230994_C / T; No. 140: chr11_10693144_C / T; No. 141: chr11_15349948_A / G; No. 142: chr11_16625823_C / T; No. 143: chr11_18721168_G / C; No. 144: chr11_20972336_T / C; No. 145: chr11_24497917_G / C; No. 146: chr11_26339202_G / A; No. 147: chr11_27296700_A / G; No. 148: chr11_32435025_C / G; No. 149: chr11_33441057_C / T; No. 150: chr11_36318555_A / G; No. 151: chr11_37339140_T / C; No. 152: chr11_52838206_C / T; No. 153: chr11_54119005_T / C; No. 154: chr11_56804196_A / G; No. 155: chr11_58931900_A / T; No. 156: chr11_65809235_C / T; No. 157: chr11_66550809_A / G; No. 158: chr11_68431341_G / C; No.159: chr11_70481364_T / C; No. 160: chr11_73036726_C / T; No. 161: chr11_75654299_T / G; No. 162: chr11_77738287_A / G; No. 163: chr11_78950768_T / C; No. 164: chr11_81229554_G / C; No. 165: chr11_83050330_T / C; No. 166: chr11_87137477_A / C; No. 167: chr11_89140638_T / A; No. 168: chr11_92067165_A / G; No. 169: chr11_94382496_T / C; No. 170: chr11_95793088_A / C; No. 171: chr11_97780273_T / A; No. 172: chr11_99798300_C / T; No. 173: chr11_103731833_C / T; No. 174: chr11_106241676_C / T; No. 175: chr11_107838926_C / T; No. 176: chr11_111011826_G / A; No. 177: chr11_112757949_A / C; No. 178: chr11_114617511_G / C; No. 179: chr11_116079333_C / A; No. 180: chr11_120057023_G / C; No. 181: chr11_120364827_T / A; No. 182: chr11_124706118_A / G; No. 183: chr11_126976297_A / G; No. 184: chr11_128521003_C / G; No. 185: chr11_131481404_T / C; No. 186: chr11_135353922_C / T; No. 187: chr11_137957589_G / A; No. 188: chr11_139017202_T / C; No. 189: chr11_141448059_G / C; No. 190: chr11_144756188_A / G; No. 191: chr11_145194302_C / G; No. 192: chr12_774806_A / G; No. 193: chr12_2086492_A / G; No. 194: chr12_6916786_G / A; No. 195: chr12_8399024_A / C; No.196: chr12_10430475_T / G; NO.197: chr12_14305278_A / G; NO.198: chr12_15193057_A / C; NO.199: chr12_17337751_A / G; NO.200: chr12_18825147_G / T; NO.201: chr12_23180825_C / T; NO.202: chr12_37317340_T / C; NO.203: chr12_39453472_A / G; NO.204: chr12_41464126_A / G; NO.205: chr12_43617713_C / T; NO.206: chr12_47470542_G / A; NO.207: chr12_47792022_T / C; NO.208: chr12_50975339_G / A; NO.209: chr12_56201604_G / A; NO.210: chr12_58053199_T / C; NO.211: chr12_60387982_T / C; NO.212: chr12_62942998_A / G; NO.213: chr12_66558415_C / T; NO.214: chr12_69638874_C / T; NO.215: chr12_70672646_G / C; NO.216: chr12_77514657_T / C; NO.217: chr12_78772634_C / G; NO.218: chr12_81184666_G / A; NO.219: chr12_83576165_T / A; NO.220: chr12_85704566_C / A; NO.221: chr12_87981040_A / G; NO.222: chr12_89528692_C / T; NO.223: chr12_92309055_C / T; NO.224: chr12_96751366_G / C; NO.225: chr12_98458891_A / G; NO.226: chr12_99549598_G / A; NO.227: chr12_103899526_T / G; NO.228: chr12_105715173_C / A; NO.229: chr12_108032307_A / G; NO.230: chr12_110475301_C / T; NO.231: chr12_112438959_C / A; NO.232: chr12_114433925_C / T; NO.233: chr12_116165020_T / C; NO.234: chr12_118201844_G / A; No. 235: chr12_120442474_G / A; No. 236: chr12_124637298_A / G; No. 237: chr12_128035195_A / C; No. 238: chr12_130586625_G / A; No. 239: chr12_133742165_T / G; No. 240: chr12_134758184_G / A; No. 241: chr12_136896353_A / T; No. 242: chr12_140959796_G / A; No. 243: chr13_2914965_C / A; No. 244: chr13_4834733_A / T; No. 245: chr13_6255419_T / G; No. 246: chr13_10247365_G / A; No. 247: chr13_10775877_T / C; No. 248: chr13_16849606_C / T; No. 249: chr13_22476663_C / T; No. 250: chr13_23090587_A / G; No. 251: chr13_35916452_C / T; No. 252: chr13_37726375_G / A; No. 253: chr13_39764112_G / A; No. 254: chr13_44755549_T / C; No. 255: chr13_45686815_T / A; No. 256: chr13_47779943_A / G; No. 257: chr13_51422117_T / G; No. 258: chr13_51919728_G / A; No. 259: chr13_55262673_A / C; No. 260: chr13_56117622_A / C; No. 261: chr13_59511888_G / A; No. 262: chr13_60162055_G / C; No. 263: chr13_62440778_C / A; No. 264: chr13_68157488_G / A; No. 265: chr13_70557715_G / A; No. 266: chr13_75334422_G / A; No. 267: chr13_76917031_G / A; No. 268: chr13_82013129_G / A; No. 269: chr13_87366012_G / A; No. 270: chr13_89287076_A / G; No. 271: chr13_91302443_C / G; No.272: chr13_93965381_T / C; No. 273: chr13_95351417_T / C; No. 274: chr13_97547087_G / A; No. 275: chr13_99778268_A / G; No. 276: chr13_102815929_A / C; No. 277: chr13_106352529_A / G; No. 278: chr13_107787942_A / G; No. 279: chr13_109869413_T / C; No. 280: chr13_114622849_T / G; No. 281: chr13_118191504_T / C; No. 282: chr13_120293292_G / T; No. 283: chr14_1450647_G / A; No. 284: chr14_2102675_T / C; No. 285: chr14_4151469_G / A; No. 286: chr14_8782843_G / A; No. 287: chr14_10466955_C / G; No. 288: chr14_16399743_T / C; No. 289: chr14_19853866_T / C; No. 290: chr14_21366071_A / G; No. 291: chr14_23536133_G / A; No. 292: chr14_27635016_C / T; No. 293: chr14_29389977_C / T; No. 294: chr14_33400926_G / A; No. 295: chr14_36153310_T / A; No. 296: chr14_38176776_A / T; No. 297: chr14_39865341_C / T; No. 298: chr14_41922046_T / C; No. 299: chr14_43536519_A / G; No. 300: chr14_46297728_A / G; No. 301: chr14_47814603_A / G; No. 302: chr14_50071882_G / A; No. 303: chr14_53215607_G / A; No. 304: chr14_54186860_G / A; No. 305: chr14_56989696_C / T; No. 306: chr14_58456578_A / G; No. 307: chr14_60666744_A / G; No. 308: chr14_63558393_G / T; No. 309: chr14_64781810_T / C; No.310: chr14_66872117_G / T; No. 311: chr14_76888411_A / C; No. 312: chr14_80883588_A / C; No. 313: chr14_83124087_G / T; No. 314: chr14_85663622_T / G; No. 315: chr14_87572686_T / C; No. 316: chr14_89656886_T / G; No. 317: chr14_91411167_A / G; No. 318: chr14_94796679_T / C; No. 319: chr14_105718067_C / A; No. 320: chr14_109106574_T / C; No. 321: chr14_110314554_G / A; No. 322: chr14_112045849_T / G; No. 323: chr14_114433261_T / A; No. 324: chr14_117511393_G / A; No. 325: chr14_120282534_A / G; No. 326: chr14_124405386_T / C; No. 327: chr14_126523745_C / T; No. 328: chr14_129471680_T / A; No. 329: chr14_131233226_G / A; No. 330: chr14_132663282_G / A; No. 331: chr15_1085160_T / G; No. 332: chr15_2080941_T / C; No. 333: chr15_4163231_C / T; No. 334: chr15_7646345_C / T; No. 335: chr15_9991074_C / T; No. 336: chr15_10684795_A / T; No. 337: chr15_13388702_T / C; No. 338: chr15_15339924_C / T; No. 339: chr15_16595692_T / C; No. 340: chr15_18668604_C / T; No. 341: chr15_21543938_C / T; No. 342: chr15_22929402_T / C; No. 343: chr15_25462584_A / C; No. 344: chr15_27800701_C / T; No. 345: chr15_29353298_T / G; No. 346: chr15_34621718_A / G; No. 347: chr15_35363189_G / T; No.348: chr15_38866570_C / T; No. 349: chr15_39659988_A / T; No. 350: chr15_42437883_T / C; No. 351: chr15_46365540_C / G; No. 352: chr15_48608121_T / C; No. 353: chr15_50527783_T / C; No. 354: chr15_54511719_G / C; No. 355: chr15_55975234_G / C; No. 356: chr15_60336613_A / G; No. 357: chr15_63030366_G / C; No. 358: chr15_64407322_A / C; No. 359: chr15_68246202_A / G; No. 360: chr15_71604537_C / T; No. 361: chr15_72571070_C / T; No. 362: chr15_74642230_T / C; No. 363: chr15_76696698_A / C; No. 364: chr15_78861456_T / C; No. 365: chr15_80847238_C / G; No. 366: chr15_83148005_G / A; No. 367: chr15_85503889_G / A; No. 368: chr15_87096529_G / A; No. 369: chr15_91267518_C / T; No. 370: chr15_93281988_A / G; No. 371: chr15_95914693_A / C; No. 372: chr15_97520514_G / A; No. 373: chr15_99658487_T / G; No. 374: chr15_112916968_G / A; No. 375: chr15_115731475_G / A; No. 376: chr15_116132427_A / C; No. 377: chr15_120074477_T / C; No. 378: chr16_2121606_C / T; No. 379: chr16_6219709_T / C; No. 380: chr16_8815466_C / G; No. 381: chr16_10920966_A / G; No. 382: chr16_12477333_T / C; No. 383: chr16_14535471_C / T; No. 384: chr16_19827297_T / C; No. 385: chr16_20975469_C / T; No.386: chr16_22980021_T / C; No. 387: chr16_25523215_A / G; No. 388: chr16_27004256_G / T; No. 389: chr16_31274989_T / G; No. 390: chr16_33193831_G / C; No. 391: chr16_35312693_G / A; No. 392: chr16_37678775_C / T; No. 393: chr16_39388184_C / T; No. 394: chr16_44220054_G / A; No. 395: chr16_45603485_A / T; No. 396: chr16_47675147_C / T; No. 397: chr16_49756061_T / A; No. 398: chr16_51842955_C / G; No. 399: chr16_54406193_A / T; No. 400: chr16_68194040_A / G; No. 401: chr16_68726627_G / T; No. 402: chr16_70612820_T / G; No. 403: chr16_76258916_G / A; No. 404: chr16_76730345_C / T; No. 405: chr16_78777729_G / A; No. 406: chr16_82167560_T / G; No. 407: chr16_83194135_T / C; No. 408: chr16_88066147_C / T; No. 409: chr17_1654853_T / C; No. 410: chr17_5797911_T / C; No. 411: chr17_6264728_T / G; No. 412: chr17_11337961_T / C; No. 413: chr17_14641356_G / C; No. 414: chr17_19106788_G / A; No. 415: chr17_21199396_T / A; No. 416: chr17_26113017_C / T; No. 417: chr17_27187081_T / C; No. 418: chr17_29949292_A / G; No. 419: chr17_31823630_G / A; No. 420: chr17_33242298_C / A; No. 421: chr17_35537730_G / A; No. 422: chr17_37834302_G / A; No. 423: chr17_39475063_C / T; No.424: chr17_56294162_A / G; No. 425: chr17_58585429_A / G; No. 426: chr17_60154711_T / C; No. 427: chr17_62958973_A / G; No. 428: chr17_65121010_A / G; No. 429: chr17_66546077_T / C; No. 430: chr17_68741775_C / T; No. 431: chr17_72552051_C / T; No. 432: chr17_75052049_A / G; No. 433: chr17_79636831_G / A; No. 434: chr17_80920927_G / A; No. 435: chr17_83218896_C / A; No. 436: chr17_85505072_C / T; No. 437: chr17_87305344_C / G; No. 438: chr17_92146525_A / G; No. 439: chr17_93651767_G / T; No. 440: chr17_99501223_C / G; No. 441: chr17_102227977_G / A; No. 442: chr17_103644866_G / A; No. 443: chr17_106034027_A / T; No. 444: chr17_107869246_G / A; No. 445: chr18_1940905_A / G; No. 446: chr18_2823165_C / T; No. 447: chr18_4611741_T / C; No. 448: chr18_6392559_C / T; No. 449: chr18_8603965_C / T; No. 450: chr18_10598190_C / A; No. 451: chr18_13239877_C / G; No. 452: chr18_15129952_A / G; No. 453: chr18_16730945_G / A; No. 454: chr18_19632206_A / G; No. 455: chr18_20738934_T / C; No. 456: chr18_22824787_A / G; No. 457: chr18_25303398_A / G; No. 458: chr18_38388123_G / A; No. 459: chr18_43217828_T / C; No. 460: chr18_43611927_G / A; No. 461: chr18_47424714_G / A; No.462: chr18_47776603_C / T; No. 463: chr18_53983536_C / T; No. 464: chr18_57381547_G / T; No. 465: chr18_59947096_T / C; No. 466: chr18_60850700_T / C; No. 467: chr18_62278629_G / A; No. 468: chr18_64303834_T / C; No. 469: chr18_66340295_G / A; No. 470: chr18_70280572_T / A; No. 471: chr18_72497034_T / C; No. 472: chr18_74138638_A / C; No. 473: chr18_74789162_G / C; No. 474: chr18_76804278_C / G; No. 475: chr18_79227342_C / T; No. 476: chr18_81818263_A / G; No. 477: chr18_82914822_G / A; No. 478: chr19_878627_G / A; No. 479: chr19_6242468_G / T; No. 480: chr19_8723458_G / A; No. 481: chr19_12438500_T / C; No. 482: chr19_14586224_C / T; No. 483: chr19_17687908_T / C; No. 484: chr19_22412796_A / C; No. 485: chr19_22928893_T / G; No. 486: chr19_41465649_A / G; No. 487: chr19_43532580_T / C; No. 488: chr19_45659181_G / T; No. 489: chr19_47756216_C / T; No. 490: chr19_50679586_T / G; No. 491: chr19_55439289_A / G; No. 492: chr19_58052690_T / C; No. 493: chr19_60128464_C / T; No. 494: chr19_62769879_C / G; No. 495: chr19_65857947_A / G; No. 496: chr2_2148823_C / G; No. 497: chr2_4386620_A / T; No. 498: chr2_6565506_A / G; No. 499: chr2_10418038_C / G; No.500: chr2_12802805_A / G; No. 501: chr2_16667250_T / C; No. 502: chr2_18760967_G / A; No. 503: chr2_20829877_G / A; No. 504: chr2_24926717_A / G; No. 505: chr2_27002052_C / T; No. 506: chr2_30066547_C / T; No. 507: chr2_31102577_C / A; No. 508: chr2_33768518_G / C; No. 509: chr2_35244300_G / T; No. 510: chr2_37412608_C / G; No. 511: chr2_39385193_G / A; No. 512: chr2_41921345_T / C; No. 513: chr2_44312578_A / G; No. 514: chr2_45602615_C / T; No. 515: chr2_49748918_T / A; No. 516: chr2_51826069_G / A; No. 517: chr2_56670848_A / C; No. 518: chr2_58375014_C / T; No. 519: chr2_60153837_G / T; No. 520: chr2_62252718_A / G; No. 521: chr2_64371708_C / A; No. 522: chr2_67111801_T / C; No. 523: chr2_69116436_T / C; No. 524: chr2_71267340_C / T; No. 525: chr2_72700423_A / G; No. 526: chr2_75069108_T / C; No. 527: chr2_76698942_A / C; No. 528: chr2_79801713_A / G; No. 529: chr2_81172420_T / A; No. 530: chr2_84874935_T / C; No. 531: chr2_85005228_G / A; No. 532: chr2_87063722_C / T; No. 533: chr2_89341172_T / C; No. 534: chr2_92025396_C / T; No. 535: chr2_93420833_C / T; No. 536: chr2_96338288_T / C; No. 537: chr2_97607328_C / T; No. 538: chr2_99590158_G / A; No. 539: chr2_103118302_A / G; No.540: chr2_104363122_A / G; No. 541: chr2_106035922_C / T; No. 542: chr2_110259213_A / T; No. 543: chr2_111986311_A / G; No. 544: chr2_114550497_T / C; No. 545: chr2_117543108_A / G; No. 546: chr2_118901801_C / T; No. 547: chr2_120633115_A / G; No. 548: chr2_124463812_A / T; No. 549: chr2_127017333_A / G; No. 550: chr2_136601850_G / T; No. 551: chr2_137358615_T / A; No. 552: chr2_139345240_G / C; No. 553: chr2_142413724_T / A; No. 554: chr2_143867914_G / T; No. 555: chr2_145444920_A / G; No. 556: chr2_147904648_G / A; No. 557: chr2_150139448_T / C; No. 558: chr2_153416337_C / T; No. 559: chr2_156310082_A / G; No. 560: chr2_160963045_G / T; No. 561: chr2_161821952_A / G; No. 562: chr2_163943516_G / C; No. 563: chr2_167641352_T / G; No. 564: chr2_168700561_T / C; No. 565: chr2_170558196_G / T; No. 566: chr2_172087527_A / C; No. 567: chr2_175043145_A / G; No. 568: chr2_176261832_A / G; No. 569: chr2_182894435_C / T; No. 570: chr2_184515084_T / C; No. 571: chr2_187245040_A / G; No. 572: chr2_189135177_G / A; No. 573: chr2_192933009_C / T; No. 574: chr2_195230972_C / T; No. 575: chr2_199300789_G / A; No. 576: chr2_201091168_T / C; No. 577: chr20_491446_C / T; No.578: chr20_2745589_T / C; No. 579: chr20_4290138_T / C; No. 580: chr20_8779827_G / C; No. 581: chr20_10392473_G / C; No. 582: chr20_12871388_C / A; No. 583: chr20_15402906_G / T; No. 584: chr20_17664630_C / A; No. 585: chr20_19805928_G / A; No. 586: chr20_20743032_C / T; No. 587: chr20_22845835_C / T; No. 588: chr20_47097622_G / T; No. 589: chr20_47717869_C / T; No. 590: chr20_50292834_C / A; No. 591: chr20_51899537_T / C; No. 592: chr20_54337086_C / A; No. 593: chr20_55976159_G / T; No. 594: chr20_59357134_C / T; No. 595: chr20_60133613_T / G; No. 596: chr20_63163206_C / T; No. 597: chr20_65012369_A / G; No. 598: chr20_69118794_G / T; No. 599: chr20_70720894_A / G; No. 600: chr20_73632813_T / C; No. 601: chr20_75138332_C / T; No. 602: chr20_76738222_A / G; No. 603: chr20_78791719_G / A; No. 604: chr20_81529878_A / T; No. 605: chr20_82912236_C / T; No. 606: chr20_84991503_A / G; No. 607: chr20_87101621_A / G; No. 608: chr3_69667_C / T; No. 609: chr3_5380703_C / T; No. 610: chr3_9830876_A / G; No. 611: chr3_11259334_A / G; No. 612: chr3_12783123_C / T; No. 613: chr3_14515725_G / A; No. 614: chr3_17627975_G / T; No. 615: chr3_18675107_C / A; No. 616: chr3_21685361_T / A; No.617: chr3_24575624_C / T; No. 618: chr3_25560615_C / T; No. 619: chr3_27870980_A / T; No. 620: chr3_30175989_T / G; No. 621: chr3_31140709_C / A; No. 622: chr3_33611207_C / T; No. 623: chr3_35663368_G / A; No. 624: chr3_38974536_C / G; No. 625: chr3_39543685_G / T; No. 626: chr3_41806860_G / T; No. 627: chr3_46139951_G / T; No. 628: chr3_47721455_A / C; No. 629: chr3_50013449_C / T; No. 630: chr3_51931788_C / T; No. 631: chr3_55159772_C / T; No. 632: chr3_56295207_G / T; No. 633: chr3_62584916_C / T; No. 634: chr3_67767334_C / A; No. 635: chr3_71130877_C / G; No. 636: chr3_72654117_A / C; No. 637: chr3_76061562_G / T; No. 638: chr3_77921617_T / C; No. 639: chr3_78981359_G / A; No. 640: chr3_82688856_G / A; No. 641: chr3_84578736_A / G; No. 642: chr3_85013610_C / T; No. 643: chr3_89193243_T / C; No. 644: chr3_91904377_T / A; No. 645: chr3_97311569_G / A; No. 646: chr3_98605623_A / G; No. 647: chr3_99505501_G / T; No. 648: chr3_102390632_G / A; No. 649: chr3_104079272_G / A; No. 650: chr3_107813485_T / G; No. 651: chr3_112037943_C / T; No. 652: chr3_116793950_T / C; No. 653: chr3_119478019_C / T; No. 654: chr3_122297796_G / A; No. 655: chr3_124512274_G / T; No.656: chr3_144904675_C / A; NO. 657: chr3_145282898_A / G; NO. 658: chr3_148336582_T / C; NO. 659: chr3_151315877_A / G; NO. 660: chr3_155468741_T / C; NO. 661: chr3_158281955_A / G; NO. 662: chr3_162643094_G / A; NO. 663: chr3_163782871_G / A; NO. 664: chr3_166102775_T / C; NO. 665: chr3_168790432_G / C; NO. 666: chr3_170628649_T / C; NO. 667: chr3_172103507_C / T; NO. 668: chr3_174542893_T / G; NO. 669: chr3_176903215_G / A; NO. 670: chr3_178300690_C / A; NO. 671: chr3_180490411_A / G; NO. 672: chr3_182445603_G / A; NO. 673: chr3_184942590_A / C; NO. 674: chr3_187752586_T / C; NO. 675: chr3_189409039_T / A; NO. 676: chr3_191942292_T / G; NO. 677: chr3_193037201_G / C; NO. 678: chr3_198281721_T / G; NO. 679: chr4_85763_A / C; NO. 680: chr4_2094135_C / T; NO. 681: chr4_5263723_A / G; NO. 682: chr4_6724497_C / T; NO. 683: chr4_8455725_A / G; NO. 684: chr4_12778006_T / A; NO. 685: chr4_16524564_A / C; NO. 686: chr4_23032753_T / C; NO. 687: chr4_24905902_G / A; NO. 688: chr4_27630887_A / G; NO. 689: chr4_29597524_T / C; NO. 690: chr4_33200151_T / C; NO. 691: chr4_35565928_T / C; NO. 692: chr4_39096005_A / G; NO. 693: chr4_39444057_A / T; NO. 694: chr4_41903472_C / A; NO.695: chr4_43600583_G / A; No. 696: chr4_46799895_C / T; No. 697: chr4_47740440_C / T; No. 698: chr4_50180447_A / C; No. 699: chr4_51891151_T / A; No. 700: chr4_54550925_T / C; No. 701: chr4_56067994_C / T; No. 702: chr4_58505714_T / C; No. 703: chr4_60303535_G / A; No. 704: chr4_62229062_T / G; No. 705: chr4_65159899_T / A; No. 706: chr4_66796081_C / T; No. 707: chr4_68439899_T / G; No. 708: chr4_73361496_T / C; No. 709: chr4_76900768_G / C; No. 710: chr4_79616317_G / T; No. 711: chr4_85099683_G / A; No. 712: chr4_87216417_C / A; No. 713: chr4_89319425_A / G; No. 714: chr4_92713489_G / A; No. 715: chr4_93598574_G / T; No. 716: chr4_97094725_T / G; No. 717: chr4_97767627_G / T; No. 718: chr4_99943087_A / G; No. 719: chr4_101994761_C / T; No. 720: chr4_104335034_G / A; No. 721: chr4_105937381_C / T; No. 722: chr4_108402834_A / G; No. 723: chr4_110724932_G / C; No. 724: chr4_112260304_T / C; No. 725: chr4_114894614_T / A; No. 726: chr4_117358722_A / G; No. 727: chr4_118279459_A / G; No. 728: chr4_132583335_C / T; No. 729: chr4_133342361_T / C; No. 730: chr4_135006264_T / C; No. 731: chr4_137637990_C / T; No. 732: chr4_140245172_T / C; No. 733: chr4_140960169_A / G; No.734: chr4_143832715_T / C; No. 735: chr4_145795981_C / A; No. 736: chr4_148116454_T / G; No. 737: chr4_149347647_T / C; No. 738: chr4_153731497_A / G; No. 739: chr4_156023948_C / A; No. 740: chr4_158015881_C / T; No. 741: chr4_160401887_G / A; No. 742: chr4_162399168_G / A; No. 743: chr4_164097326_T / C; No. 744: chr4_167146012_T / A; No. 745: chr4_168822375_A / C; No. 746: chr4_170068496_T / C; No. 747: chr4_172458943_C / T; No. 748: chr4_177173357_A / G; No. 749: chr4_178286860_G / A; No. 750: chr4_180703483_C / T; No. 751: chr5_119160_C / A; No. 752: chr5_3578662_A / T; No. 753: chr5_4182780_G / A; No. 754: chr5_7000670_C / T; No. 755: chr5_10191737_T / C; No. 756: chr5_10940247_T / C; No. 757: chr5_12476014_T / C; No. 758: chr5_15376479_A / G; No. 759: chr5_19430548_G / A; No. 760: chr5_22989280_T / C; No. 761: chr5_25555235_A / G; No. 762: chr5_27219570_T / C; No. 763: chr5_29093832_C / T; No. 764: chr5_31349137_G / A; No. 765: chr5_33189444_C / T; No. 766: chr5_35299651_T / C; No. 767: chr5_38211388_G / A; No. 768: chr5_40575633_A / G; No. 769: chr5_43865047_A / G; No. 770: chr5_48600990_A / G; No. 771: chr5_51583354_T / G; No. 772: chr5_52460341_A / G; No.773: chr5_54020459_G / C; No. 774: chr5_57204567_T / C; No. 775: chr5_58115832_A / C; No. 776: chr5_60397638_G / A; No. 777: chr5_62978529_G / C; No. 778: chr5_64364395_C / A; No. 779: chr5_67012266_A / C; No. 780: chr5_68633492_G / A; No. 781: chr5_72817283_G / A; No. 782: chr5_75344263_T / A; No. 783: chr5_76754500_G / A; No. 784: chr5_81826499_T / A; No. 785: chr5_91530082_A / G; No. 786: chr5_95103308_T / A; No. 787: chr5_95379276_T / C; No. 788: chr5_99665088_T / C; No. 789: chr5_102402052_C / T; No. 790: chr5_104240572_C / A; No. 791: chr5_105750082_C / T; No. 792: chr5_107943231_G / A; No. 793: chr5_110433086_T / A; No. 794: chr5_112412054_T / C; No. 795: chr5_114108394_T / A; No. 796: chr5_117674143_G / A; No. 797: chr5_120253038_G / A; No. 798: chr5_124539995_T / A; No. 799: chr5_126677505_C / G; No. 800: chr5_128524694_C / T; No. 801: chr5_130872601_T / C; No. 802: chr5_143165688_T / C; No. 803: chr5_145124176_T / G; No. 804: chr5_150274914_C / T; No. 805: chr5_156363091_C / T; No. 806: chr5_159671928_G / A; No. 807: chr5_166767314_G / T; No. 808: chr5_170323360_A / C; No. 809: chr5_172235756_G / T; No. 810: chr5_174772028_A / G; No. 811: chr5_176443628_T / C; No.812: chr5_178784383_C / A; No. 813: chr5_182307360_C / A; No. 814: chr5_184387774_C / T; No. 815: chr5_184766993_G / A; No. 816: chr5_186692586_A / C; No. 817: chr5_189167789_C / T; No. 818: chr5_190779552_C / T; No. 819: chr5_194060333_T / C; No. 820: chr5_194845170_C / T; No. 821: chr6_421338_G / A; No. 822: chr6_3661064_G / A; No. 823: chr6_4707987_G / C; No. 824: chr6_7758979_C / G; No. 825: chr6_8488083_A / T; No. 826: chr6_11871797_T / C; No. 827: chr6_12976392_A / G; No. 828: chr6_16620943_G / C; No. 829: chr6_18842267_A / C; No. 830: chr6_21927851_G / A; No. 831: chr6_23115151_A / C; No. 832: chr6_24930255_A / T; No. 833: chr6_29636149_G / A; No. 834: chr6_31186747_G / C; No. 835: chr6_33366289_T / C; No. 836: chr6_39783977_T / C; No. 837: chr6_41482589_G / A; No. 838: chr6_45625260_T / C; No. 839: chr6_56881145_G / C; No. 840: chr6_58490814_A / T; No. 841: chr6_61922629_A / C; No. 842: chr6_63656293_C / G; No. 843: chr6_64428131_G / A; No. 844: chr6_66523388_A / T; No. 845: chr6_68418628_C / T; No. 846: chr6_71710056_C / T; No. 847: chr6_73108282_G / A; No. 848: chr6_74664927_C / G; No. 849: chr6_77420405_C / T; No. 850: chr6_79087255_A / G; No.851: chr6_81127034_A / G; No. 852: chr6_88410909_G / C; No. 853: chr6_89695377_G / A; No. 854: chr6_91258579_A / G; No. 855: chr6_94319036_T / C; No. 856: chr6_95373549_G / A; No. 857: chr6_97609674_A / G; No. 858: chr6_101595910_A / G; No. 859: chr6_104857027_T / A; No. 860: chr6_106361902_C / G; No. 861: chr6_112308079_T / C; No. 862: chr6_116399345_A / T; No. 863: chr6_120738261_T / C; No. 864: chr6_122620845_T / G; No. 865: chr6_124436747_T / C; No. 866: chr6_128402278_A / G; No. 867: chr6_129335227_A / G; No. 868: chr6_130650707_T / C; No. 869: chr6_133486819_G / C; No. 870: chr6_135368219_A / G; No. 871: chr6_136960453_T / C; No. 872: chr6_138979575_C / T; No. 873: chr6_144247211_G / A; No. 874: chr6_147017645_G / A; No. 875: chr6_148418119_G / A; No. 876: chr6_149421883_A / G; No. 877: chr6_151428063_A / C; No. 878: chr6_154197246_C / G; No. 879: chr6_155971715_C / A; No. 880: chr6_157874281_A / G; No. 881: chr6_161678593_T / C; No. 882: chr6_164671690_C / G; No. 883: chr6_166313755_G / A; No. 884: chr6_168145524_G / A; No. 885: chr6_170042721_G / T; No. 886: chr6_172309868_T / C; No. 887: chr6_174121127_C / G; No. 888: chr6_179673637_T / C; No.889: chr6_181437940_A / G; NO.890: chr6_185480152_A / G; NO.891: chr7_1046581_T / A; NO.892: chr7_2625508_A / G; NO.893: chr7_5772333_C / G; NO.894: chr7_6336028_C / T; NO.895: chr7_9431483_T / C; NO.896: chr7_11239153_G / A; NO.897: chr7_12555415_T / C; NO.898: chr7_19750007_C / T; NO.899: chr7_22942441_T / A; NO.900: chr7_25174673_T / C; NO.901: chr7_29281611_G / A; NO.902: chr7_35013962_T / C; NO.903: chr7_35445442_T / C; NO.904: chr7_39390958_G / A; NO.905: chr7_41541931_T / C; NO.906: chr7_43553283_G / T; NO.907: chr7_46344089_C / T; NO.908: chr7_47806481_A / C; NO.909: chr7_51833827_C / T; NO.910: chr7_54797202_C / T; NO.911: chr7_56105000_G / A; NO.912: chr7_71753451_G / T; NO.913: chr7_72655041_A / G; NO.914: chr7_75692875_C / T; NO.915: chr7_77435261_A / G; NO.916: chr7_84799484_A / T; NO.917: chr7_85658083_G / A; NO.918: chr7_88790609_G / A; NO.919: chr7_89557602_G / A; NO.920: chr7_101545733_G / T; NO.921: chr7_102180847_C / T; NO.922: chr7_104212224_T / A; NO.923: chr7_105828527_A / T; NO.924: chr7_107872654_C / T; NO.925: chr7_111146733_C / T; NO.926: chr7_113223687_A / G; NO.927: chr7_116209020_A / G; NO.928: chr7_120262141_A / G; NO.929: chr7_123593065_A / C; NO.930: chr7_124892952_T / G; NO.931: chr7_126563101_C / T; NO.932: chr7_128998598_T / C; NO.933: chr7_131593960_G / A; NO.934: chr7_133257437_C / T; NO.935: chr7_135018737_G / A; NO.936: chr7_136877942_A / G; NO.937: chr7_138928317_C / T; NO.938: chr7_141451706_G / A; NO.939: chr7_144989474_G / A; NO.940: chr7_148501159_A / G; NO.941: chr7_149274888_G / A; NO.942: chr7_153288812_T / C; NO.943: chr7_153769981_C / T; NO.944: chr7_155498813_C / T; NO.945: chr7_157645948_A / T; NO.946: chr7_162268256_A / G; NO.947: chr7_164031460_G / C; NO.948: chr7_169398032_G / A; NO.949: chr7_170446682_A / G; NO.950: chr7_172285811_C / G; NO.951: chr7_175102193_C / A; NO.952: chr7_176241547_G / A; NO.953: chr8_1444348_G / A; NO.954: chr8_3142903_C / T; NO.955: chr8_4262136_T / G; NO.956: chr8_6504853_C / A; NO.957: chr8_11961280_T / G; NO.958: chr8_16760485_T / C; NO.959: chr8_19246139_G / A; NO.960: chr8_20747793_G / A; NO.961: chr8_23044612_A / G; NO.962: chr8_25396596_T / C; NO.963: chr8_27194860_A / T; NO.964: chr8_29024189_T / C; NO.965: chr8_32078158_C / T; NO.966: chr8_34329892_T / G; NO.967: chr8_35391852_C / T; No. 968: chr8_37605423_A / C; No. 969: chr8_39392622_C / A; No. 970: chr8_44028410_G / A; No. 971: chr8_45630309_C / T; No. 972: chr8_58556799_G / A; No. 973: chr8_60604375_A / G; No. 974: chr8_62189763_C / T; No. 975: chr8_66013594_A / T; No. 976: chr8_66729372_G / A; No. 977: chr8_69053205_T / C; No. 978: chr8_71339414_C / T; No. 979: chr8_77442409_C / T; No. 980: chr8_80841243_T / C; No. 981: chr8_83872208_A / G; No. 982: chr8_84985067_T / A; No. 983: chr8_87331473_A / G; No. 984: chr8_89164865_A / C; No. 985: chr8_91800836_C / T; No. 986: chr8_94062851_T / G; No. 987: chr8_95364433_A / T; No. 988: chr8_97530942_G / A; No. 989: chr8_99915385_C / G; No. 990: chr8_103429438_T / C; No. 991: chr8_103699161_A / C; No. 992: chr8_105733551_C / T; No. 993: chr8_109226588_G / T; No. 994: chr8_113556716_A / G; No. 995: chr8_114867882_A / C; No. 996: chr8_116743342_C / T; No. 997: chr8_119193593_T / A; No. 998: chr8_121295867_A / G; No. 999: chr8_122434790_A / T; No. 1000: chr8_124841155_G / A; No. 1001: chr8_126556661_C / T; No. 1002: chr8_129162334_C / T; No. 1003: chr8_130788062_C / T; No. 1004: chr8_132797818_A / G; No. 1005: chr8_136036390_A / G; No.1006: chr8_137606992_T / C; No. 1007: chr8_139334158_C / A; No. 1008: chr8_141539527_A / G; No. 1009: chr8_143057589_A / G; No. 1010: chr8_147155292_A / G; No. 1011: chr8_148699828_C / A; No. 1012: chr8_151376607_C / A; No. 1013: chr8_153420646_G / A; No. 1014: chr8_155525357_G / A; No. 1015: chr9_2092745_G / C; No. 1016: chr9_4229138_G / C; No. 1017: chr9_6243643_C / T; No. 1018: chr9_8395121_A / T; No. 1019: chr9_10426225_C / T; No. 1020: chr9_12863694_T / C; No. 1021: chr9_14968285_T / C; No. 1022: chr9_20835244_C / A; No. 1023: chr9_22819804_C / T; No. 1024: chr9_26644979_A / C; No. 1025: chr9_29205645_A / G; No. 1026: chr9_31950891_A / G; No. 1027: chr9_33312994_G / A; No. 1028: chr9_35484507_A / G; No. 1029: chr9_37868028_G / A; No. 1030: chr9_55440217_A / G; No. 1031: chr9_55980871_G / T; No. 1032: chr9_58152192_C / T; No. 1033: chr9_62020963_G / A; No. 1034: chr9_62862130_A / G; No. 1035: chr9_66664758_C / T; No. 1036: chr9_68547963_T / C; No. 1037: chr9_70580297_C / T; No. 1038: chr9_72843917_C / A; No. 1039: chr9_74627303_A / C; No. 1040: chr9_77109210_T / G; No. 1041: chr9_78803965_G / T; No. 1042: chr9_81410890_C / T; No. 1043: chr9_85102183_C / T; No.1044: chr9_89103702_C / T; NO.1045: chr9_89164900_C / T; NO.1046: chr9_93335424_G / A; NO.1047: chr9_95669011_G / A; NO.1048: chr9_97673153_T / C; NO.1049: chr9_99649161_G / T; NO.1050: chr9_102735309_C / A; NO.1051: chr9_103855688_C / T; NO.1052: chr9_106207663_C / T; NO.1053: chr9_107851687_A / G; NO.1054: chr9_114066560_A / T; NO.1055: chr9_116755730_A / G; NO.1056: chr9_118280908_A / G; NO.1057: chr9_121372258_G / A; NO.1058: chr9_122571039_C / A; NO.1059: chr9_124656097_C / T; NO.1060: chr9_126442925_T / C; NO.1061: chr9_129073398_T / C; NO.1062: chr9_131008821_T / C; NO.1063: chr9_133067865_G / A; NO.1064: chr9_134815879_C / A; NO.1065: chr9_136813696_T / C; NO.1066: chr9_139248734_A / G; NO.1067: chr9_140951054_C / T; NO.1068: chr9_143043430_T / G; NO.1069: chr9_146267936_C / T; NO.1070: chrX_3454760_T / C; NO.1071: chrX_4834225_G / A; NO.1072: chrX_6273562_G / A; NO.1073: chrX_8342503_C / T; NO.1074: chrX_10416051_T / G; NO.1075: chrX_12464350_A / C; NO.1076: chrX_14596209_G / C; NO.1077: chrX_18723643_T / A; NO.1078: chrX_22356948_G / T; NO.1079: chrX_26417481_T / C; NO.1080: chrX_28173903_C / A; NO.1081:chrX_29823389_A / G; NO.1082:chrX_31571791_C / T; NO.1083:chrX_34579760_G / A; NO.1084:chrX_35287427_A / G; NO.1085:chrX_40574974_C / G; NO.1086:chrX_44518674_G / A; NO.1087:chrX_50140263_T / C; NO.1088:chrX_51844271_A / G; NO.1089:chrX_57408146_G / A; NO.1090:chrX_58090782_C / T; NO.1091:chrX_71167443_C / T; NO.1092:chrX_75683949_G / A; NO.1093:chrX_76712259_G / A; NO.1094:chrX_78809126_T / C; NO.1095:chrX_81443105_A / G; NO.1096:chrX_84175356_G / T; NO.1097:chrX_87130319_C / T; NO.1098:chrX_89530851_A / G; NO.1099:chrX_91232296_T / C; NO.1100:chrX_93488827_C / G; NO.1101:chrX_95649304_C / G; NO.1102:chrX_97435544_A / G; NO.1103:chrX_99743509_A / G; NO.1104:chrX_102467822_C / G; NO.1105:chrX_104260957_A / C; NO.1106:chrX_105998824_A / G; NO.1107:chrX_107795990_A / G; NO.1108:chrX_111420954_G / A; NO.1109:chrX_113406574_T / C; NO.1110:chrX_114004296_T / C; NO.1111:chrX_116271431_T / G; NO.1112:chrX_119014181_C / G; NO.1113:chrX_120562148_C / T; NO.1114:chrX_122924553_A / G; NO.1115:chrX_125648105_G / T; NO.1116:chrX_132520689_A / T; NO.1117:chrX_132686848_T / C; NO.1118:chrX_136833646_A / G; No. 1119:chrX_140909238_A / G; No. 1120:chrX_140950446_A / T; No. 1121:chrX_144480163_G / A; No. 1122:chrX_146201857_A / G; No. 1123:chrX_147291157_A / G; No. 1124:chrX_152674904_T / A; No. 1125:chrX_153456784_G / A; No. 1126:chrX_155949284_A / G; No. 1127:chrX_159473596_G / A; No. 1128:chrX_160002659_G / T; No. 1129:chrX_162439114_T / C; No. 1130:chrY_12674649_T / C; No. 1131:chrY_12674693_G / C; No. 1132:chrY_12703045_T / C; No. 1133:chrY_12703060_A / G; No. 1134:chrY_12703074_A / T; No. 1135:chrY_12703681_C / T; No. 1136:chrY_13795339_C / T; No. 1137:chrY_13830913_G / A; No. 1138:chrY_13831647_G / A; No. 1139:chrY_13831727_T / C; No. 1140:chrY_13832429_A / G; No. 1141:chrY_13834827_G / C; No. 1142:chrY_13850122_A / C; No. 1143:chrY_13879517_T / G; No. 1144:chrY_13882520_T / C; No. 1145:chrY_13901814_A / G; No. 1146:chrY_13916431_G / A; No. 1147:chrY_13916462_T / C; No. 1148:chrY_13917814_C / T; No. 1149:chrY_13917838_A / G; No. 1150:chrY_13922404_T / C; No. 1151:chrY_13929951_T / C; No. 1152:chrY_13931945_G / A; No. 1153:chrY_13935030_G / T; No. 1154:chrY_13935558_C / G; No. 1155:chrY_13939685_A / C; No.1156: chrY_13966366_A / G; No. 1157: chrY_13972512_C / A; No. 1158: chrY_13974709_T / C; No. 1159: chrY_13977380_C / G; No. 1160: chrY_13977540_T / G; No. 1161: chrY_13995570_C / G; No. 1162: chrY_14033586_G / C; No. 1163: chrY_14051349_G / T; No. 1164: chrY_14071629_T / C; No. 1165: chrY_14085294_C / T; No. 1166: chrY_14088919_T / C; No. 1167: chrY_14088962_T / C; No. 1168: chrY_14096542_G / T; No. 1169: chrY_14096554_G / C; No. 1170: chrY_14103750_T / C; No. 1171: chrY_14103802_A / G; No. 1172: chrY_14103838_C / G; No. 1173: chrY_14107904_G / T; No. 1174: chrY_14107905_A / T; No. 1175: chrY_14108911_A / C; No. 1176: chrY_14117071_G / T; No. 1177: chrY_14118273_G / A; No. 1178: chrY_14118565_T / C; No. 1179: chrY_14122172_C / T; No. 1180: chrY_14149426_T / C; No. 1181: chrY_14150341_C / T; No. 1182: chrY_14157007_A / G; No. 1183: chrY_14158782_G / A; No. 1184: chrY_14162772_T / C; No. 1185: chrY_14175335_A / T; No. 1186: chrY_14175580_T / C; No. 1187: chrY_14176274_C / T; No. 1188: chrY_14178609_C / G; No. 1189: chrY_14181564_G / C; No. 1190: chrY_14184444_A / G; No. 1191: chrY_14184489_T / C; No. 1192: chrY_14193850_C / T; No. 1193: chrY_14193959_T / C; No.1194: chrY_14194572_C / A; No. 1195: chrY_14197818_A / G; No. 1196: chrY_14205890_G / A; No. 1197: chrY_14205901_G / C; No. 1198: chrY_14205911_T / G; No. 1199: chrY_14210599_T / G; No. 1200: chrY_14210838_T / A; No. 1201: chrY_14212258_C / G; No. 1202: chrY_14212275_A / C; No. 1203: chrY_14228250_A / G; No. 1204: chrY_14228314_G / A; No. 1205: chrY_14229265_C / T; No. 1206: chrY_14230705_G / A; No. 1207: chrY_14232253_C / A; No. 1208: chrY_14232306_C / T; No. 1209: chrY_14232695_C / G; No. 1210: chrY_14232880_A / T; No. 1211: chrY_14232883_T / G; No. 1212: chrY_14233571_A / G; No. 1213: chrY_14233606_A / G; No. 1214: chrY_14236627_G / A; No. 1215: chrY_14237292_T / C; No. 1216: chrY_14237303_G / A; No. 1217: chrY_14237341_C / A; No. 1218: chrY_14241406_T / G; No. 1219: chrY_14244447_A / G; No. 1220: chrY_14249451_T / A; No. 1221: chrY_14250194_A / C; No. 1222: chrY_14252268_C / A; No. 1223: chrY_14252300_C / G; No. 1224: chrY_14253025_A / G; No. 1225: chrY_14254242_G / A; No. 1226: chrY_14258562_A / T; No. 1227: chrY_14263002_G / A; No. 1228: chrY_14265350_G / C; No. 1229: chrY_14269440_G / C; No. 1230: chrY_14286643_G / A; No. 1231: chrY_14286652_G / A; No.1232: chrY_14286667_G / A; NO.1233: chrY_14286711_G / A; NO.1234: chrY_14286993_G / T; NO.1235: chrY_14288072_G / A; NO.1236: chrY_14288147_C / T; NO.1237: chrY_14289684_A / G; NO.1238: chrY_14289776_C / A; NO.1239: chrY_14299935_A / C; NO.1240: chrY_14302259_T / A; NO.1241: chrY_14302262_G / A; NO.1242: chrY_14302348_C / A; NO.1243: chrY_14302417_G / A; NO.1244: chrY_14302593_C / G; NO.1245: chrY_14305106_T / C; NO.1246: chrY_14305241_T / G; NO.1247: chrY_14308565_C / T; NO.1248: chrY_14311274_A / G; NO.1249: chrY_14312593_G / A; NO.1250: chrY_14314037_A / G; NO.1251: chrY_14314127_A / G; NO.1252: chrY_14314155_T / C; NO.1253: chrY_14319778_C / A; NO.1254: chrY_14319843_C / T; NO.1255: chrY_14319919_T / C; NO.1256: chrY_14320426_T / C; NO.1257: chrY_14322293_G / A; NO.1258: chrY_14322296_T / A; NO.1259: chrY_14322357_C / G; NO.1260: chrY_14329426_G / A; NO.1261: chrY_14332613_G / C; NO.1262: chrY_14341635_A / G; NO.1263: chrY_14341675_A / C; NO.1264: chrY_14341733_C / T; NO.1265: chrY_14345365_G / C; NO.1266: chrY_14348588_T / A; NO.1267: chrY_14348622_A / G; NO.1268: chrY_14348659_C / T; NO.1269: chrY_14348710_T / C; NO.1270: chrY_14348717_T / C; No. 1271: chrY_14348774_A / G; No. 1272: chrY_14351592_G / T; No. 1273: chrY_14351662_G / C; No. 1274: chrY_14364166_C / T; No. 1275: chrY_14366558_T / C; No. 1276: chrY_14366656_A / T; No. 1277: chrY_14368451_A / G; No. 1278: chrY_14368594_G / A; No. 1279: chrY_14368748_A / G; No. 1280: chrY_14368952_G / T; No. 1281: chrY_14373937_G / A; No. 1282: chrY_14389543_C / T; No. 1283: chrY_14390691_T / A; No. 1284: chrY_14390739_C / G; No. 1285: chrY_14390749_G / C; No. 1286: chrY_14390757_A / G; No. 1287: chrY_14390763_T / C; No. 1288: chrY_14391047_C / A; No. 1289: chrY_14392370_C / A; No. 1290: chrY_14396533_T / A; No. 1291: chrY_14396540_C / A; No. 1292: chrY_14396578_C / A; No. 1293: chrY_14400305_C / T; No. 1294: chrY_14400353_A / G; No. 1295: chrY_14400406_T / C; No. 1296: chrY_14403819_A / C; No. 1297: chrY_14405166_G / A; No. 1298: chrY_14408704_C / T; No. 1299: chrY_14408715_T / C; No. 1300: chrY_14409664_T / G; No. 1301: chrY_14409811_C / T; No. 1302: chrY_14409831_G / A; No. 1303: chrY_14410002_G / A; No. 1304: chrY_14410102_C / T; No. 1305: chrY_14411038_T / C; No. 1306: chrY_14411201_C / G; No. 1307: chrY_14411558_C / G; No.1308: chrY_14414031_G / T; No. 1309: chrY_14414127_C / T; No. 1310: chrY_14414185_G / T; No. 1311: chrY_14416152_T / C; No. 1312: chrY_14416385_C / T; No. 1313: chrY_14422992_A / C; No. 1314: chrY_14423037_C / G; No. 1315: chrY_14423667_T / C; No. 1316: chrY_14423701_A / G; No. 1317: chrY_14423749_C / T; No. 1318: chrY_14423757_C / G; No. 1319: chrY_14424377_G / A; No. 1320: chrY_14424409_G / T; No. 1321: chrY_14424463_T / C; No. 1322: chrY_14425058_G / T; No. 1323: chrY_14425070_A / G; No. 1324: chrY_14425082_G / A; No. 1325: chrY_14425094_A / G; No. 1326: chrY_14427509_G / A; No. 1327: chrY_14438194_T / C; No. 1328: chrY_14439526_T / G; No. 1329: chrY_14440750_T / C; No. 1330: chrY_14440849_G / A; No. 1331: chrY_14442307_C / T; No. 1332: chrY_14450411_G / A; No. 1333: chrY_14450429_A / G; No. 1334: chrY_14450677_C / G; No. 1335: chrY_14453917_A / G; No. 1336: chrY_14456416_C / G; No. 1337: chrY_14456473_G / A; No. 1338: chrY_14456486_G / A; No. 1339: chrY_14456518_A / G; No. 1340: chrY_14456587_A / C; No. 1341: chrY_14461176_G / A; No. 1342: chrY_14466113_T / G; No. 1343: chrY_14470981_C / T; No. 1344: chrY_14470990_G / A; No. 1345: chrY_14477996_A / G; No.1346: chrY_14479302_A / G; No. 1347: chrY_14479338_G / A; No. 1348: chrY_14479352_T / G; No. 1349: chrY_14479395_A / T; No. 1350: chrY_14479417_T / A; No. 1351: chrY_14479433_G / A; No. 1352: chrY_14479434_T / G; No. 1353: chrY_14479464_T / C; No. 1354: chrY_14481734_T / A; No. 1355: chrY_14482939_T / G; No. 1356: chrY_14483245_T / G; No. 1357: chrY_14493355_A / C; No. 1358: chrY_14493515_A / G; No. 1359: chrY_14493690_G / A; No. 1360: chrY_14493723_A / G; No. 1361: chrY_14503591_G / A; No. 1362: chrY_14505877_A / G; No. 1363: chrY_14505898_T / C; No. 1364: chrY_14513602_G / C; No. 1365: chrY_14516954_C / T; No. 1366: chrY_14519160_A / C; No. 1367: chrY_14519784_A / G; No. 1368: chrY_14519839_A / G; No. 1369: chrY_14519860_T / G; No. 1370: chrY_14519907_C / A; No. 1371: chrY_14519916_T / C; No. 1372: chrY_14524013_A / G; No. 1373: chrY_14524138_C / T; No. 1374: chrY_14524150_C / T; No. 1375: chrY_14524772_A / T; No. 1376: chrY_14524803_A / G; No. 1377: chrY_14524946_A / G; No. 1378: chrY_14525016_C / A; No. 1379: chrY_14529066_G / A; No. 1380: chrY_14530256_T / C; No. 1381: chrY_14531950_T / G; No. 1382: chrY_14541123_C / T; No. 1383: chrY_14541311_A / G; No.1384: chrY_14541462_T / A; NO.1385: chrY_14548784_G / A; NO.1386: chrY_14550241_C / T; NO.1387: chrY_14550316_T / A; NO.1388: chrY_14550675_C / T; NO.1389: chrY_14553911_C / T; NO.1390: chrY_14553913_G / T; NO.1391: chrY_14554360_A / G; NO.1392: chrY_14554439_G / A; NO.1393: chrY_14561798_A / G; NO.1394: chrY_14565009_C / T; NO.1395: chrY_14565477_G / A; NO.1396: chrY_14565945_C / G; NO.1397: chrY_14566044_G / A; NO.1398: chrY_14569102_T / C; NO.1399: chrY_14570304_T / C; NO.1400: chrY_14570342_G / T; NO.1401: chrY_14570400_G / T; NO.1402: chrY_14571522_A / C; NO.1403: chrY_14575427_C / A; NO.1404: chrY_14591930_C / G; NO.1405: chrY_14593424_A / T; NO.1406: chrY_14596205_C / A; NO.1407: chrY_14600718_T / C; NO.1408: chrY_14605194_T / C; NO.1409: chrY_14605252_T / A; NO.1410: chrY_14612891_A / G; NO.1411: chrY_14622478_G / A; NO.1412: chrY_14635576_C / T; NO.1413: chrY_14640894_C / T; NO.1414: chrY_14640970_G / A; NO.1415: chrY_14642908_G / C; NO.1416: chrY_14644945_T / C; NO.1417: chrY_14649111_A / G; NO.1418: chrY_14658717_A / C; NO.1419: chrY_14674659_C / A; NO.1420: chrY_14674660_A / G; NO.1421: chrY_14695863_T / C; NO.1422: chrY_14699225_A / T; NO.1423: chrY_14706069_C / T; NO.1424: chrY_14713544_C / T; NO.1425: chrY_14717053_G / A; NO.1426: chrY_14725802_G / A; NO.1427: chrY_14726018_A / C; NO.1428: chrY_14726058_C / T; NO.1429: chrY_14727434_C / A; NO.1430: chrY_14728441_A / G; NO.1431: chrY_14728575_C / T; NO.1432: chrY_14730818_T / A; NO.1433: chrY_14732233_G / C; NO.1434: chrY_14739400_A / G; NO.1435: chrY_14741867_G / T; NO.1436: chrY_14742253_C / T; NO.1437: chrY_14757492_G / A; NO.1438: chrY_14768821_A / G; NO.1439: chrY_14770080_T / A; NO.1440: chrY_14770233_A / G; NO.1441: chrY_14771118_G / A; NO.1442: chrY_14771175_G / A; NO.1443: chrY_14774033_C / T; NO.1444: chrY_14774063_T / C; NO.1445: chrY_14774724_C / A; NO.1446: chrY_14774847_T / C; NO.1447: chrY_14774948_G / C; NO.1448: chrY_14775055_C / G; NO.1449: chrY_14777042_T / C; NO.1450: chrY_14777140_G / C; NO.1451: chrY_14778059_G / T; NO.1452: chrY_14778066_T / C; NO.1453: chrY_14780048_C / T; NO.1454: chrY_14780104_C / T; NO.1455: chrY_14783074_C / T; NO.1456: chrY_14783290_C / T; NO.1457: chrY_14784989_G / A; NO.1458: chrY_14785045_G / A; NO.1459: chrY_14785152_A / C; NO.1460: chrY_14785439_G / C; No. 1461: chrY_14787845_A / G; No. 1462: chrY_14788738_A / G; No. 1463: chrY_14792434_A / G; No. 1464: chrY_14792495_G / A; No. 1465: chrY_14792497_G / A; No. 1466: chrY_14793583_C / G; No. 1467: chrY_14794477_C / T; No. 1468: chrY_14794546_T / C; No. 1469: chrY_14794664_C / T; No. 1470: chrY_14795217_G / A; No. 1471: chrY_14796721_A / T; No. 1472: chrY_14796857_G / A; No. 1473: chrY_14797688_A / G; No. 1474: chrY_14803100_A / G; No. 1475: chrY_14803358_A / C; No. 1476: chrY_14804991_A / G; No. 1477: chrY_14807220_A / G; No. 1478: chrY_14818834_T / A; No. 1479: chrY_14818960_C / G; No. 1480: chrY_14825696_C / G; No. 1481: chrY_14826472_A / G; No. 1482: chrY_14827150_C / T; No. 1483: chrY_14829341_A / G; No. 1484: chrY_14831356_G / A; No. 1485: chrY_14832545_G / A; No. 1486: chrY_14837547_A / G; No. 1487: chrY_14839488_T / C; No. 1488: chrY_14839571_T / C; No. 1489: chrY_14843891_C / A; No. 1490: chrY_14843984_A / C; No. 1491: chrY_14851112_G / A; No. 1492: chrY_14852706_G / C; No. 1493: chrY_14852710_G / C; No. 1494: chrY_14852854_G / A; No. 1495: chrY_14860131_A / G; No. 1496: chrY_14860193_A / C; No. 1497: chrY_14860310_C / A; No.1498: chrY_14860365_C / A; NO.1499: chrY_14860507_T / C; NO.1500: chrY_14872583_C / T; NO.1501: chrY_14872682_T / C; NO.1502: chrY_14874468_G / A; NO.1503: chrY_14874557_A / G; NO.1504: chrY_14874825_A / G; NO.1505: chrY_14884169_T / C; NO.1506: chrY_14884196_C / T; NO.1507: chrY_14898253_A / G; NO.1508: chrY_14901801_C / T.

[0009] A set of probes for detecting the above 1508 SNP sites. The probes are designed according to the nucleotide sequences of 50 bp on both flanks of the above SNP sites and using GenoBaits Designer software. The probes are synthesized by Shijiazhuang Borui Biotechnology Co., Ltd. and modified with biotin labeling. Probe design principle: probe length 110 nt; GC content between 30-70%; number of homologous regions ≤ 5.

[0010] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0011] 1. The 1508 cynomolgus macaque-specific SNP sites disclosed and screened in the present invention have high polymorphism, can realize rapid identification, paternity testing and lifelong tracking identification of cynomolgus macaque individuals, and have more information than microsatellites used in the past, and the genetic relationship reflected is more accurate.

[0012] 2. The liquid chip adopted in the present invention is a GBTS-targeted sequencing genotype detection technology, which has the advantages of low cost, high accuracy, high detection sensitivity, high throughput, etc., and is suitable for paternity testing and individual identification research of large-scale samples.

[0013] 3. The test of the cynomolgus macaque paternity testing liquid chip developed in the present invention on three different populations shows that its average site detection rate is 99.472%, indicating that the liquid chip has excellent site capture efficiency, high site capture rate and good stability among samples.

[0014] 4. The present invention can greatly improve the genetic detection ability and accuracy of cynomolgus macaques, and can significantly reduce the genetic detection cost, providing important technical support for improving the breeding management of cynomolgus macaques and optimizing the population structure. Description of the Drawings

[0015] Figure 1 It is a distribution map of the 1508 SNP sites of the present invention;

[0016] Figure 2 Results graph of site capture rate test for cynomolgus macaque samples of three different groups. Detailed implementation manners

[0017] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0018] Reference Figure 1 - Figure 2 :

[0019] Example 1

[0020] In this embodiment, the 1508 polymorphic SNP locus combinations specific to cynomolgus macaques are derived from 83 genome resequencing data of the Kunming Institute of Zoology, Chinese Academy of Sciences, and their physical positions are determined according to a male T2T cynomolgus macaque reference genome assembled by this research group. The obtaining method includes the following steps:

[0021] S1. Collect blood samples from 83 cynomolgus macaques, extract DNA and perform sequencing, and each sample is sequenced with 90G (30X) data;

[0022] S2. After the raw data is filtered by removing adapters, use the BWA software (mem mode) to align it to the cynomolgus macaque reference genome, remove duplicates by the SAMtools software, and obtain population SNP loci by the GATK software;

[0023] S3. Perform the first hard filtering on the above population SNP loci using the filtering criteria recommended by the GATK official, and then further screen according to the following conditions: remove non-biallelic SNP loci and non-INDEL loci; the minimum sequencing depth > 5X; the locus deletion rate < 5%; the minor allele frequency (MAF) > 0.2; the locus heterozygosity < 60%;

[0024] S4. According to the principle of uniform distribution, preferentially screen loci with high average polymorphic information content (PIC) values, or loci with the largest MAF value of the target SNP within the segment.

[0025] Example 2

[0026] Perform site capture rate tests on 333 cynomolgus macaque samples from the Kunming Institute of Zoology, Chinese Academy of Sciences (Kunming Institute), 30 cynomolgus macaque samples from Suzhou Xishan Biotechnology Co., Ltd. (Xishan), and 20 cynomolgus macaque samples from the Institute of Medical Biology, Chinese Academy of Medical Sciences (Institute of Medical Biology) respectively. The specific steps are as follows:

[0027] S1. Use a DNA extraction kit to extract DNA, use 1% agarose gel electrophoresis to analyze the purity and integrity of DNA, and then use Qubit to accurately quantify the DNA concentration;

[0028] S2. Use a DNA fragmentation enzyme to fragment the DNA, use an End Repair reagent to repair the ends of the fragmented DNA, and use a DNA ligase to ligate the sequencing adapter to the DNA fragment that has been end-repaired to obtain a ligation product;

[0029] S3. Add the ligation product to a sequencing primer with a Barcode and a high-fidelity PCR reaction system for PCR amplification. The amplified product after purification with carboxyl magnetic beads can be used for probe hybridization experiments;

[0030] S4. Take 500 ng of the completed DNA library, add Panel, Block I and Block II mixture and concentrate to completely dryness, then add 2X Hyb Buffer and Hyb Buffer Enhancer, incubate at 65 °C for 2 hours to complete the hybridization reaction, then add DNAProbe Beads, wash and remove the unbound DNA, and only retain the target DNA fragments hybridized with Panel. Enrich and purify the target fragments by PCR amplification to complete the construction of the hybridization capture library;

[0031] S5. After the construction of the hybridization capture library is completed, first use Qubit 2.0 for preliminary quantification, and use qPCR to accurately quantify the effective concentration of the library to ensure the library quality. After the library passes the detection, enter the next-generation sequencing stage;

[0032] S6. During the data analysis process, for the sites with sequencing coverage lower than the set threshold, they are marked as missing sites, and for the sites with successful coverage and qualified data quality, they are marked as detected sites. The results obtained are shown in Table 2:

[0033] Table 2

[0034]

[0035]

[0036]

[0037]

[0038]

[0039]

[0040] Analysis of test results:

[0041] Among the test samples of the above 383 cynomolgus monkey samples, the number of samples with a detection rate lower than 90% is zero, and the number of qualified samples is 383. The detection rate is between 96.088% and 99.934%, and the average detection rate is 99.472%. From the data, Figure 2 The test result diagrams of the capture rates of cynomolgus monkey samples from three different units shown indicate that the liquid-phase chip system for cynomolgus monkey paternity testing established by the present invention has excellent site capture efficiency and good stability of sites among samples.

[0042] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cynomolgus monkey-specific SNP locus combination, characterized in that: Includes 1508 sites mapped on the cynomolgus macaque reference genome.

2. A liquid phase chip, characterized in that: The liquid phase chip is used to capture the SNP sites in the cynomolgus monkey-specific SNP site combination as claimed in claim 1.

3. Use of the cynomolgus monkey-specific SNP locus combination according to claim 1 in cynomolgus monkey individual identification and paternity testing.

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