Automatic egg cell transfer device

Abstract

The utility model discloses an automatic egg cell transfer device, relates to egg cell transfer technical field, including transfer mechanism, connecting mechanism and pipetting mechanism, transfer mechanism includes suction subassembly, needle cylinder and first connector, when transferring egg cell, suction subassembly aligns the egg cell, adjust power mechanism, make the needle cylinder produce negative pressure, and the first connector connects the suction subassembly and the needle cylinder. The egg cells are sucked into the needle cylinder through the suction assembly, the power mechanism is adjusted again, the pressure in the needle cylinder is increased, the egg cells are discharged out through the suction assembly, and transfer of the egg cells is achieved. Oval cells can be sucked and discharged through the needle head only by simply adjusting the power mechanism, the power mechanism can be repeatedly used, high-temperature sterilization and other operations on other mechanisms cannot affect the experiment precision, operation is easy and convenient, cost is saved, and working efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of egg cell transfer, in particular to an automatic egg cell transfer device. Background Art

[0002] An egg cell (ovum, egg) is the reproductive cell of a female organism. Both animals and seed plants produce egg cells. In higher organisms, egg cells are produced by the ovaries. In the reproductive department of a hospital, when conducting related experimental operations on frozen eggs, first-generation test tubes, etc., a series of operations such as selecting, aspirating, and transferring egg cells are required for front-end processing. The common method in the laboratory is to observe and select eggs under a microscope, then aspirate the eggs with a pipette or syringe, and then blow and transfer them to a new storage environment.

[0003] A patent with a publication date of October 27, 2023 and a publication number of CN219907642U discloses a cell aspiration device. The cell aspiration device includes a syringe body and a piston rod. An anti-backward-pushing mechanism is arranged outside the syringe body. The anti-backward-pushing mechanism includes a protective shell, a backward-pushing plate, a clamping block, and an elastic component. The protective shell is fixedly connected to the outer wall of the syringe body. One end of the backward-pushing plate is fixedly connected to the piston rod. The other end of the backward-pushing plate is slidably inserted through a support plate, and the other end of the backward-pushing plate is slidably inserted through the protective shell. The clamping block is arranged inside the protective shell through the elastic component. A plurality of clamping grooves are equidistantly opened at the top end of the backward-pushing plate, and the clamping block is slidably inserted through the inner cavity of the clamping groove. The utility model uses the setting method of cooperating the protective shell, the backward-pushing plate, the clamping block, and the elastic component, which can control the speed and quality of sampling, and makes the piston rod fixed by clamping with the clamping groove, preventing the movement of the piston rod from affecting the spillage of cell fluid, and is relatively convenient to use.

[0004] Egg cell-related experiments are carried out in a sterile environment. Experimental instruments need to go through a strict sterilization and disinfection process before use, and during the experiment, the accuracy should be ensured as much as possible. The above scheme considers preventing the movement of the piston rod from affecting the spillage of cell fluid, but in actual use, there may be omissions when manually operating for a long time, and the cumbersome manual operation will reduce the efficiency.

[0005] Therefore, it is necessary to provide an automatic egg cell transfer device. Summary of the Utility Model

[0006] The embodiment of the present application provides an automatic egg cell transfer device, which can improve problems such as the decrease in attention caused by excessive doctor operations in the laboratory stage and the reduction of the experimental speed due to cumbersome operations in the related technology.

[0007] An embodiment of the present application provides an automated egg cell transfer device, which includes a transfer mechanism, a connection mechanism, and a pipetting mechanism. The transfer mechanism includes a suction component, a syringe barrel, and a first connector. One end of the connection mechanism is connected to the pipetting mechanism, the syringe barrel is connected to the other end of the connection mechanism through the first connector, and the suction component is connected to the end of the syringe barrel away from the connection mechanism.

[0008] An embodiment of the present application provides an automated egg cell transfer device. When it is necessary to transfer an egg cell, the suction component is aligned with the egg cell, the power mechanism is adjusted to generate negative pressure in the syringe barrel, and the egg cell is sucked into the syringe barrel through the suction component. Then, the power mechanism is adjusted again to increase the pressure in the syringe barrel, and the egg cell is blown out through the suction component to achieve the transfer of the egg cell.

[0009] The above technical solution in the embodiment of the present application has at least the following technical effects: The connection mechanism can filter bacteria and at the same time prevent the excessive suction force of the power mechanism. Only by simply adjusting the power mechanism to reduce or increase the pressure of the syringe barrel, the egg cell can be sucked and discharged through the needle. The power mechanism can be reused, and operations such as high-temperature sterilization of other mechanisms will not affect the accuracy of the experiment. The operation is simple, the cost is saved, and the work efficiency is improved.

[0010] In some embodiments, the suction component includes a needle and a fixing plug. The needle is fixedly connected to the fixing plug. The needle is in an obtuse L shape, and the diameter of the end of the needle away from the fixing plug is smaller than the diameter of the end close to the fixing plug. The fixing plug is slidably connected to the end of the syringe barrel away from the connection mechanism.

[0011] In some embodiments, the pipetting mechanism includes an adjustment component, a pipette tip, a handle, an upper shell, and a second connector. The handle is arranged on the upper shell, and the handle and the upper shell are integrally formed. Grooves are provided on both the upper shell and the handle, and the adjustment component is arranged in the grooves. The upper shell is provided with a through hole, the pipette tip is arranged on the upper shell, and the pipette tip is communicated with the upper shell through the through hole. The second connector is arranged on the pipette tip.

[0012] In some embodiments, the adjustment assembly includes an adjustment button, a battery, a controller, a chip board, an information collector, a motor, a display screen, an electric suction and discharge pump, and an air chamber. The adjustment button includes an inhalation button, an exhalation button, and a range pump speed adjustment button. The motor is drivingly connected to the electric suction and discharge pump. One end of the controller is electrically connected to the motor, and the other end of the controller is electrically connected to the information collector and the battery. The information collector is electrically connected to the chip board. The display screen is disposed on the upper shell and is electrically connected to the information collector. The air chamber is disposed in the groove. One end of the air chamber is connected to the electric suction and discharge pump, and the other end of the air chamber is communicated with the suction nozzle. The inhalation button and the exhalation button are both disposed on the handle, and both the inhalation button and the exhalation button are electrically connected to the controller. The range pump speed adjustment button is disposed on the upper shell and is electrically connected to the chip board.

[0013] In some embodiments, an exhaust hole is formed in the upper shell, and the exhaust hole is communicated with the exhaust end of the electric suction pump.

[0014] In some embodiments, the connection mechanism includes a first connection pipe, a second connection pipe, and a filter. A filter membrane is disposed in the filter. One end of the first connection pipe is communicated with the syringe through a first connector, the other end of the first connection pipe is communicated with one end of the filter, one end of the second connection pipe is communicated with the other end of the filter, and the other end of the second connection pipe is communicated with the suction nozzle through a second connector. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0016] Figure 1 It is a schematic diagram of the overall structure of an automated oocyte transfer device provided by an embodiment of the present application;

[0017] Figure 2 It is a schematic diagram of the overall structure of another perspective of an automated oocyte transfer device provided by an embodiment of the present application;

[0018] Figure 3 It is a sectional view of the transfer mechanism of an automated oocyte transfer device provided by an embodiment of the present application;

[0019] Figure 4A sectional view of the pipetting mechanism of an automated egg cell transfer device provided by an embodiment of the present application.

[0020] Among them, the reference numerals in the figure are as follows:

[0021] 1. Transfer mechanism; 11. Suction assembly; 111. Needle; 112. Fixed plug; 12. Syringe; 13. First connector; 2. Connection mechanism; 21. First connecting pipe; 22. Second connecting pipe; 23. Filter; 3. Pipetting mechanism; 31. Adjustment assembly; 311. Adjustment button; 3111. Inhalation button; 3112. Discharge button; 3113. Range pump speed adjustment button; 312. Battery; 313. Controller; 314. Chip board; 315. Information collector; 316. Motor; 317. Display screen; 318. Electric suction and discharge pump; 319. Air chamber; 32. Nozzle; 33. Handle; 34. Upper shell; 341. Through hole; 342. Exhaust hole; 35. Second connector. Detailed implementation manners

[0022] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application clearer, the present application will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the present application. The terms "including" and "having" and any variations thereof in the specification and claims of the present application and the above drawings are intended to cover non-exclusive inclusion.

[0024] It should be noted that when an element is referred to as "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0025] It should be understood that the orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application.

[0026] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of this application, "a plurality of" means two or more, unless otherwise specifically defined.

[0027] In this application, "and / or" is merely an associative relationship describing associated objects, indicating that three relationships may exist; for example, A and / or B may represent: A exists alone, A and B exist simultaneously, and B exists alone. Additionally, the character " / " in this text generally represents an "or" relationship between the associated objects before and after.

[0028] It should be noted that in this application, words such as "in some embodiments", "exemplarily", and "for example" are used to give examples, illustrations, or explanations. Any embodiment or design solution described as "in some embodiments", "exemplarily", or "for example" in this application should not be construed as being more preferred or having more advantages than other embodiments or design solutions. Rather, the use of words such as "in some embodiments", "exemplarily", and "for example" is intended to present relevant concepts in a specific manner, meaning that the specific features, structures, or characteristics described in combination with the embodiments may be included in at least one embodiment of this application. The appearance of the above words at various positions in the specification does not necessarily refer to the same embodiment, nor are they independent or alternative embodiments mutually exclusive with other embodiments. Those skilled in the art explicitly and implicitly understand that the embodiments described herein may be combined with other embodiments.

[0029] The egg cell (ovum, egg) is the reproductive cell of female organisms. Both animals and seed plants produce egg cells. In higher organisms, egg cells are produced by the ovaries. In the reproductive department of a hospital, during related experimental operations such as freezing eggs and first-generation in vitro fertilization, a series of operations such as selecting, aspirating, and transferring egg cells are required for front-end processing. The commonly used method in the laboratory is to observe and select eggs under a microscope, then aspirate the eggs using a pipette or syringe, and then blow and transfer them to a new environment for preservation.

[0030] A cell aspiration device in the related art includes a syringe body and a piston rod. An anti-backward push mechanism is arranged outside the syringe body. The anti-backward push mechanism includes a protective shell, a backward push plate, a clamping block and an elastic component. The protective shell is fixedly connected to the outer wall of the syringe body. By using the cooperation of the protective shell, the backward push plate, the clamping block and the elastic component, the speed and quality of the extracted sample can be controlled, and the piston rod is fixed by being clamped with a clamping groove, preventing the movement of the piston rod from affecting the spillage of the cell fluid, which is relatively convenient to use. However, in actual use, the cell aspiration device needs to manually push the piston repeatedly for a long time, and the manual operation is cumbersome, which will reduce the efficiency.

[0031] Based on this, in order to improve the problem of reduced efficiency caused by the frequent manual operation of the device in the related art, the embodiments of the present application provide the following solutions.

[0032] Please refer to Figure 1 simultaneously. The embodiments of the present application provide an automated egg cell transfer device, which includes a transfer mechanism 1, a connection mechanism 2 and a pipetting mechanism 3. The transfer mechanism 1 includes a suction component 11, a syringe 12 and a first connector 13. One end of the connection mechanism 2 is connected to the pipetting mechanism 3, the syringe 12 is connected to the other end of the connection mechanism 2 through the first connector 13, the suction component 11 is connected to the end of the syringe 12 away from the connection mechanism 2, and the pipetting mechanism 3 changes the pressure in the syringe 12 through the connection mechanism 2, so that the egg cell enters and exits the syringe 12 through the suction component 11.

[0033] In some embodiments, please refer to Figure 3 simultaneously. The suction component 11 includes a needle head 111 and a fixing plug 112. The needle head 111 is fixedly connected to the fixing plug 112. The needle head 111 is in an obtuse L shape, and the diameter of the end of the needle head 111 away from the fixing plug 112 is smaller than the diameter of the end close to the fixing plug 112. The fixing plug 112 is slidably connected to the end of the syringe 12 away from the connection mechanism 2.

[0034] With such a setting, the obtuse L-shaped needle head 111 makes the actual operation more convenient. The needle head 111 approaches the egg cell, and the negative pressure generated by the pipetting mechanism 3 is used to suck the egg cell into the syringe 12 for temporary storage. Then, when the positive pressure generated by the pipetting mechanism 3 is used to blow the egg cell out of the syringe 12, and the design of the fixing plug 112 allows the egg cell to smoothly reach the through hole 341 of the needle head 111 from the syringe 12 without getting stuck in a flat surface or a concave surface.

[0035] In some embodiments, please refer to Figure 4, the pipetting mechanism 3 includes an adjustment component 31, a pipette tip 32, a handle 33, an upper shell 34, and a second connector 35. The handle 33 is arranged on the upper shell 34, and the handle 33 and the upper shell 34 are integrally formed. Grooves are provided on both the upper shell 34 and the handle 33, and the adjustment component 31 is arranged in the grooves. The upper shell 34 is provided with a through hole 341, and the pipette tip 32 is adhered to the upper shell 34 and is communicated with the upper shell 34 through the through hole 341.

[0036] With such an arrangement, the handle 33 and the upper shell 34 are integrally formed and both have grooves, which allows the wires in the adjustment component 31 to be connected to each other inside the grooves without external wires.

[0037] In some embodiments, please also refer to Figure 4 , the adjustment component 31 includes an adjustment button 311, a battery 312, a controller 313, a chip board 314, an information collector 315, a motor 316, a display screen 317, an electric suction and discharge pump 318, and an air chamber 319. The adjustment button 311 includes a suction button 3111, a discharge button 3112, and a range pump speed adjustment button 3113. The motor 316 is in transmission connection with the electric suction and discharge pump 318. One end of the controller 313 is electrically connected to the motor 316 in an electrical signal manner, and the other end of the controller 313 is electrically connected to the information collector 315 and the battery 312 in an electrical signal manner. The information collector 315 is electrically connected to the chip board 314. The display screen 317 is adhered to the upper shell 34 and is electrically connected to the information collector 315 in an electrical signal manner. The air chamber 319 is arranged in the groove. One end of the air chamber 319 is connected to the electric suction and discharge pump 318, and the other end of the air chamber 319 is communicated with the pipette tip 32. The suction button 3111 and the discharge button 3112 are both slidably connected to the handle 33 and are both electrically connected to the controller 313. The range pump speed adjustment button 3113 is slidably connected to the upper shell 34 and is electrically connected to the chip board 314.

[0038] With such a setting, the battery 312 enables the pipetting mechanism 3 to be repeatedly charged and discharged. After the battery 312 is fully charged, press the range pump speed adjustment button 3113. The chip board 314 obtains the adjustment information, feeds back the adjustment information to the controller 313, and at the same time displays the adjustment information in a digital form on the display screen 317. Observe the display screen 317, adjust the required range and pump speed. Hold the handle 33 and press the suction button 3111. The controller 313 receives the feedback. The controller 313 controls the motor 316, and the motor 316 drives the electric suction and discharge pump 318 to generate negative pressure in the air chamber 319, and through the connecting mechanism 2, the syringe 12 also generates negative pressure. At this time, bring the needle 111 close to the egg cell, and the egg cell can be smoothly sucked into the syringe 12. When the suction volume reaches the set range, continue to press the suction button 3111, and the controller 313 will not control the motor 316 to perform subsequent steps. Then press the discharge button 3112. The controller 313 controls the motor 316, and the motor 316 drives the electric suction and discharge pump 318 to generate positive pressure in the air chamber 319, and through the connecting mechanism 2, the syringe 12 also generates positive pressure to discharge the egg cell from the syringe 12. Similarly, when the discharge volume reaches the set range, continue to press the discharge button 3112, and the controller 313 will not control the motor 316 to perform subsequent steps.

[0039] Optionally, in some embodiments, please also refer to Figure 4 , an exhaust hole 342 is formed in the upper shell 34, and the exhaust hole 342 is communicated with the exhaust end of the electric suction pump.

[0040] With such a setting, the electric suction device can exchange gas with the outside world to achieve pressure balance and ensure the normal operation of the device.

[0041] Optionally, in some embodiments, please also refer to Figure 1 and Figure 2 , the connecting mechanism 2 includes a first connecting pipe 21, a second connecting pipe 22 and a filter 23. A filter membrane is provided in the filter 23. One end of the first connecting pipe 21 is communicated with the syringe 12 through a first connector 13. The other end of the first connecting pipe 21 is communicated with one end of the filter 23. One end of the second connecting pipe 22 is communicated with the other end of the filter 23. The other end of the second connecting pipe 22 is communicated with the nozzle 32 through a second connector 35.

[0042] With such a setting, the filter 23 plays a role in preventing excessive liquid suction and liquid from entering the pipetting device and damaging the motor 316. At the same time, the filter 23 can be sterilized by high temperature to ensure the aseptic condition of the egg cell transfer operation. The first connecting pipe 21 and the second connecting pipe 22 communicate the air chamber 319 with the syringe 12 to balance the pressure between the two, which is equivalent to extending the range of the nozzle 32.

[0043] The above are only the preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included within the protection scope of the present application.

Claims

1. An automated oocyte transfer device, characterized in that: The invention comprises a transfer mechanism (1), a connection mechanism (2) and a pipetting mechanism (3); the transfer mechanism (1) comprises a suction assembly (11), a syringe (12) and a first connector (13); one end of the connection mechanism (2) is connected to the pipetting mechanism (3); the syringe (12) is connected to the other end of the connection mechanism (2) via the first connector (13); and the suction assembly (11) is connected to the end of the syringe (12) away from the connection mechanism (2).

2. An automated oocyte transfer device according to claim 1, characterized in that: The suction assembly (11) comprises a needle (111) and a fixed plug (112); the needle (111) is fixedly connected to the fixed plug (112); the needle (111) is in an obtuse L-shape; the diameter of the end of the needle (111) away from the fixed plug (112) is smaller than the diameter of the end close to the fixed plug (112); the fixed plug (112) is slidably connected to the end of the syringe (12) away from the connecting mechanism (2).

3. An automated oocyte transfer device according to claim 2, characterized in that: The pipetting mechanism (3) comprises an adjusting component (31), a suction nozzle (32), a handle (33), an upper shell (34) and a second connector (35); the handle (33) is arranged on the upper shell (34); the handle (33) and the upper shell (34) are integrally formed; both the upper shell (34) and the handle (33) are provided with a groove; the adjusting component (31) is arranged in the groove; the upper shell (34) is provided with a through hole (341); the suction nozzle (32) is arranged on the upper shell (34); and the suction nozzle (32) is connected with the upper shell (34) through the through hole (341); and the second connector (35) is arranged on the suction nozzle (32).

4. An automated oocyte transfer device according to claim 3, characterized in that: The regulating component (31) comprises a regulating button (311), a battery (312), a controller (313), a chip board (314), an information collector (315), a motor (316), a display screen (317), an electric suction and discharge pump (318), and an air cavity (319); the regulating button (311) comprises a suction button (3111), a discharge button (3112), and a range pump speed regulating button (3113); the motor (316) is transmission-connected to the electric suction and discharge pump (318); one end of the controller (313) is electrically signal-connected to the motor (316); the other end of the controller (313) is electrically signal-connected to the information collector (315) and the battery (312); the information collector (315) is electrically signal-connected to the chip board (319); 14), the display screen (317) is arranged on the upper shell (34), and the display screen (317) is electrically connected to the information collector (315), the air cavity (319) is arranged in the groove, one end of the air cavity (319) is connected to the electric suction and discharge pump (318), and the other end of the air cavity (319) is connected to the suction nozzle (32), the suction button (3111) and the discharge button (3112) are both arranged on the handle (33), and the suction button (3111) and the discharge button (3112) are both electrically connected to the controller (313), the range pump speed adjustment button (3113) is arranged on the upper shell (34), and the range pump speed adjustment button (3113) is electrically connected to the chip board (314).

5. An automated oocyte transfer device according to claim 4, characterized in that: The upper shell (34) is provided with an exhaust hole (342), and the exhaust hole (342) is connected to the exhaust end of the electric suction and discharge pump.

6. The automated oocyte transfer device according to claim 3, characterized in that: The connecting mechanism (2) comprises a first connecting tube (21), a second connecting tube (22) and a filter (23), wherein a filter membrane is arranged in the filter (23), one end of the first connecting tube (21) is connected to the syringe (12) via a first connector (13), the other end of the first connecting tube (21) is connected to one end of the filter (23), one end of the second connecting tube (22) is connected to the other end of the filter (23), and the other end of the second connecting tube (22) is connected to the suction nozzle (32) via a second connector (35).

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