Batch dyeing device for transmission electron microscope samples

Abstract

The utility model belongs to the technical field of electron microscope sample processing tools, and particularly relates to a transmission electron microscope sample batch dyeing device which comprises a supporting mechanism, a sample dripping mechanism and a control mechanism, the supporting mechanism comprises a base, supporting columns and inserting columns, the supporting columns are arranged on the two sides of the top of the base, and the inserting columns are arranged on the tops of the supporting columns; the sample dripping mechanism is arranged on the supporting column, the sample dripping mechanism comprises a cylinder, a round pipe, needle heads, connecting plates and inserting holes, the top of the cylinder is communicated with the round pipe, the needle heads are uniformly distributed at the bottom of the cylinder, the connecting plates are arranged on the two sides of the cylinder, and the inserting holes connected with the inserting columns are formed in the connecting plates; a needle cylinder type sample dripping mechanism is adopted, needle heads are evenly distributed at the bottom of a cylinder, a control mechanism is arranged on the cylinder, a piston is pushed through a push rod to push out a sample, the sample is evenly extruded out of the needle heads, and batch dyeing is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of electron microscope sample processing tools, in particular to a batch staining device for transmission electron microscope samples. Background Technique

[0002] The negative staining sample preparation technique utilizes the principle that heavy metal elements have a stronger ability to scatter electrons than light elements that make up biological samples. Some substances with high electron density in the staining solution (such as heavy metal salts, etc.) are combined with the background of biological samples, improving the ability of the background to scatter electrons, so as to contrast the bright biological samples against a dark background.

[0003] There are various negative staining sample preparation methods, and the commonly used ones include the hanging drop method, the floating method, the carbon film-negative staining method, etc. Usually, the operation is to drop the sample on the grid or float the grid on the sample droplet.

[0004] When dropping the sample on the grid, it is necessary for the staff to drop the sample one by one through a syringe. The operation is cumbersome and batch staining cannot be carried out. Therefore, this application proposes a batch staining device for transmission electron microscope samples. Content of the Utility Model

[0005] The purpose of this part is to outline some aspects of the implementation mode of the utility model and briefly introduce some preferred implementation modes. Simplifications or omissions may be made in this part, as well as in the abstract of the specification and the name of the utility model of this application, to avoid obscuring the purpose of this part, the abstract of the specification and the name of the utility model. However, such simplifications or omissions cannot be used to limit the scope of the utility model.

[0006] In view of the above and / or problems existing in the existing batch staining device for transmission electron microscope samples, the present utility model is proposed.

[0007] Therefore, the purpose of the present utility model is to provide a batch staining device for transmission electron microscope samples. The utility model adopts a syringe-type sample dropping mechanism, with evenly distributed needles arranged at the bottom of the cylinder, and a control mechanism is arranged on the cylinder. The sample is pushed out by a push rod to push the piston, so that the sample is evenly extruded from the needles to achieve batch staining.

[0008] To solve the above technical problems, according to one aspect of the present utility model, the following technical solutions are provided:

[0009] A batch staining device for transmission electron microscope samples, which includes:

[0010] A support mechanism, including a base, columns and insertion posts. Columns are arranged on both sides of the top of the base, and insertion posts are arranged on the tops of the columns;

[0011] The dropping mechanism is arranged on the pillar. The dropping mechanism includes a cylinder, a circular tube, a needle, a connecting plate and a jack. The top of the cylinder is communicated with the circular tube. Needles are evenly distributed at the bottom of the cylinder. Connecting plates are arranged on both sides of the cylinder, and jacks connected to the inserting posts are opened on the connecting plates.

[0012] The control mechanism is arranged in the circular tube. The control mechanism includes a push rod, a piston and a push handle. The lower end of the push rod is provided with a piston connected to the inner wall of the circular tube, and the top of the push rod is provided with a push handle.

[0013] As a preferred scheme of a batch staining device for transmission electron microscope samples of the present invention, wherein: a placing mechanism is arranged at the center of the top of the base. The placing mechanism includes a collecting cup and a supporting mesh. The supporting mesh is arranged at the opening of the top of the collecting cup.

[0014] As a preferred scheme of a batch staining device for transmission electron microscope samples of the present invention, wherein: a reinforcing rib is arranged between the top of the base and the pillar.

[0015] As a preferred scheme of a batch staining device for transmission electron microscope samples of the present invention, wherein: a return spring is arranged between the push handle and the circular tube. In the natural state of the return spring, the piston is located at the top end of the inner cavity of the circular tube.

[0016] As a preferred scheme of a batch staining device for transmission electron microscope samples of the present invention, wherein: the cylinder and the circular tube are made of an integral transparent part, and liquid level identification scales are arranged on the outer walls of the cylinder and the circular tube.

[0017] As a preferred scheme of a batch staining device for transmission electron microscope samples of the present invention, wherein: anti-slip convex stripes are arranged on the outer wall of the circular tube.

[0018] As a preferred scheme of a batch staining device for transmission electron microscope samples of the present invention, wherein: an anti-slip pad is arranged at the bottom of the collecting cup.

[0019] Compared with the prior art: The present invention adopts a syringe-type dropping mechanism. Needles are evenly distributed at the bottom of the cylinder, and a control mechanism is arranged on the cylinder. The sample is pushed out by pushing the piston with the push rod, so that the sample is evenly extruded from the needles to realize batch staining. Description of the Drawings

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail below with reference to the drawings and detailed embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained according to these drawings without creative efforts. Among them:

[0021] Figure 1 This is a schematic axonometric view of the structure of the present utility model;

[0022] Figure 2 This is a schematic view of the support mechanism structure of the present utility model;

[0023] Figure 3 This is a schematic view of the sample dropping mechanism structure of the present utility model;

[0024] Figure 4 This is a schematic view of the control mechanism structure of the present utility model;

[0025] Figure 5 This is a schematic view of the placement mechanism structure of the present utility model.

[0026] In the figure: 100 support mechanism, 110 base, 120 pillar, 130 insertion post, 200 sample dropping mechanism, 210 cylinder, 220 round tube, 230 needle head, 240 connecting plate, 250 jack, 300 control mechanism, 310 push rod, 320 piston, 330 push handle, 340 return spring, 400 placement mechanism, 410 collection cup, 420 support net. Specific embodiments

[0027] To make the above objects, features, and advantages of the present utility model more obvious and understandable, the following will describe the specific embodiments of the present utility model in detail with reference to the accompanying drawings.

[0028] In the following description, many specific details are set forth to fully understand the present utility model. However, the present utility model can also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the connotation of the present utility model. Therefore, the present utility model is not limited by the specific embodiments disclosed below.

[0029] Secondly, the present utility model will be described in detail with reference to the schematic diagrams. When detailing the embodiments of the present utility model, for the convenience of explanation, the cross-sectional views showing the device structure will be enlarged locally out of the general proportion, and the schematic diagrams are only examples and should not limit the scope of protection of the present utility model herein. In addition, in actual production, three-dimensional spatial dimensions including length, width, and depth should be included.

[0030] To make the purpose, technical solutions, and advantages of the present utility model clearer, the following will further describe the embodiments of the present utility model in detail with reference to the accompanying drawings.

[0031] The present utility model provides a transmission electron microscope sample batch staining device, which adopts a syringe-type sample dropping mechanism. Needle heads are uniformly arranged at the bottom of the cylinder, and a control mechanism is arranged on the cylinder. The sample is pushed out by pushing the piston with a push rod, so that the sample is uniformly extruded from the needle heads to achieve batch staining. Please refer toFigures 1 - 5 , including: a support mechanism 100, a sample dropping mechanism 200, and a control mechanism 300.

[0032] The support mechanism 100 includes a base 110, columns 120, and insertion columns 130. Columns 120 are provided on both sides of the top of the base 110, and insertion columns 130 are provided on the tops of the columns 120.

[0033] Among them, circular columns 120 are fixedly provided on both sides of the top of the base 110, and concentric insertion columns 130 are provided on the tops of the columns 120.

[0034] The sample dropping mechanism 200 is arranged on the columns 120. The sample dropping mechanism 200 includes a cylinder 210, a circular tube 220, a needle 230, a connecting plate 240, and a jack 250. The top of the cylinder 210 is connected to the circular tube 220, needles 230 are evenly distributed at the bottom of the cylinder 210, connecting plates 240 are provided on both sides of the cylinder 210, and jacks 250 for connecting to the insertion columns 130 are opened on the connecting plates 240.

[0035] Among them, the cylinder 210, the circular tube 220, and the needle 230 form a syringe barrel structure. Different from a conventional syringe barrel, the diameter of the cylinder 210 is larger than that of the circular tube 220, and the needles 230 are distributed in a rectangular array.

[0036] The control mechanism 300 is arranged in the circular tube 220. The control mechanism 300 includes a push rod 310, a piston 320, and a push handle 330. The lower end of the push rod 310 is provided with a piston 320 connected to the inner wall of the circular tube 220, and the top of the push rod 310 is provided with a push handle 330.

[0037] Among them, the push rod 310, the piston 320, and the push handle 330 form a syringe push rod structure. By pushing the push handle 330 to push the push rod 310, the piston 320 is driven to push in the circular tube 220, and the sample stored in the cylinder 210 is discharged.

[0038] Since the sample needs to be dropped on the grid, in order to facilitate the support of the grid, a placement mechanism 400 is provided at the center of the top of the base 110. The placement mechanism 400 includes a collection cup 410 and a grid support 420. The grid support 420 is provided at the opening at the top of the collection cup 410.

[0039] An anti-slip pad is provided at the bottom of the collection cup 410. The grid is supported by the grid support 420. After sample dropping, the liquid seeps into the collection cup 410 through the grid support 420 for collection.

[0040] To improve the structural strength, a reinforcing rib is provided between the top of the base 110 and the columns 120 to prevent the structure from breaking.

[0041] Since it is necessary to control the amount of sample dropped, a return spring 340 is provided between the push handle 330 and the round tube 220. In the natural state of the return spring 340, the piston 320 is located at the top of the inner cavity of the round tube 220. After pushing the push handle 330 and then releasing it, the push handle 330 is reset and moves upward under the action of the return spring 340.

[0042] To facilitate observing the remaining amount of the internal sample, the cylinder 210 and the round tube 220 are made of an integrated transparent part, and liquid level identification scales are provided on the outer walls of the cylinder 210 and the round tube 220, which is convenient for observing the remaining amount of the internal sample.

[0043] Since it is necessary to be grasped and used, anti-slip ridges are provided on the outer wall of the round tube 220, which can be grasped to operate the round tube 220.

[0044] During specific use, the piston 320 is pulled outwards to absorb the sample through the needle 230. Then, the specimen grid is placed on the support grid 420, the push handle is pressed down, the push rod 310 is pushed by the push handle 330, driving the piston 320 to move in the round tube 220, discharging the sample stored in the cylinder 210, so that the sample is evenly dropped on the specimen grid. The push handle 330 is released, and the push handle 330 is reset and moves upward under the action of the return spring 340 to prevent excessive dripping of the sample.

[0045] Although the present invention has been described above with reference to the embodiments, various improvements can be made to it and its components can be replaced with equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed by the present invention can be combined with each other in any way, and the exhaustive description of these combinations is omitted in this specification only for the sake of saving space and resources. Therefore, the present invention is not limited to the specific embodiments disclosed in the text, but includes all technical solutions falling within the scope of the claims.

Claims

1. A transmission electron microscope sample batch staining device, characterized in that: include: A support mechanism (100) comprises a base (110), a support column (120) and an insertion column (130), wherein the support columns (120) are arranged on both sides of the top of the base (110), and the insertion column (130) is arranged on the top of the support column (120); A sample dropping mechanism (200) is arranged on the support column (120), the sample dropping mechanism (200) comprises a cylinder (210), a circular tube (220), a needle (230), a connecting plate (240) and a plug hole (250), the top of the cylinder (210) is connected to the circular tube (220), the bottom of the cylinder (210) is provided with needles (230) evenly distributed, connecting plates (240) are arranged on both sides of the cylinder (210), and the connecting plates (240) are provided with plug holes (250) connected to the plug column (130); A control mechanism (300) is arranged in the circular tube (220), and comprises a push rod (310), a piston (320) and a push handle (330). The piston (320) connected to the inner wall of the circular tube (220) is arranged at the lower end of the push rod (310), and the push handle (330) is arranged at the top of the push rod (310).

2. A transmission electron microscope sample batch staining device according to claim 1, characterized in that: A placement mechanism (400) is arranged at the top center of the base (110), and the placement mechanism (400) comprises a collection cup (410) and a support net (420). The support net (420) is arranged at the top opening of the collection cup (410).

3. A transmission electron microscope sample batch staining device according to claim 1, characterized in that: A reinforcing rib is provided between the top of the base (110) and the support (120).

4. A transmission electron microscope sample batch staining device according to claim 1, characterized in that: A return spring (340) is provided between the push handle (330) and the circular tube (220). When the return spring (340) is in a natural state, the piston (320) is located at the top end of the inner cavity of the circular tube (220).

5. The transmission electron microscope sample batch staining device according to claim 1, characterized in that: The cylinder (210) and the circular tube (220) are made of an integrated transparent component, and the outer walls of the cylinder (210) and the circular tube (220) are provided with liquid level identification scales.

6. A transmission electron microscope sample batch staining device according to claim 1, characterized in that: The outer wall of the circular tube (220) is provided with anti-slip convex patterns.

7. A transmission electron microscope sample batch staining device according to claim 2, characterized in that: The bottom of the collection cup (410) is provided with an anti-slip pad.

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