Sampling device for tumor organoid culture
By designing a tumor organoid sampling device that includes a piercing hollow needle and a lever mechanism, the problem of easily destroying the entire tumor tissue when separating the internal structure from the tumor tissue in the prior art is solved, and precise sampling and freezing are achieved, reducing resource waste.
Patent Information
- Application Number
- CN202421866468.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-05
- Publication Date
- 2025-06-03
- Estimated Expiration
- 2034-08-05
AI Technical Summary
Existing devices can easily destroy the entire tumor tissue when they are separated from the tumor tissue, resulting in waste of resources.
A sampling device for tumor organoid culture was designed, including a base, a stent, a sleeve, a syringe, a needle holder, a piercing hollow needle, a linkage pull rod, a return spring and a hand press rod. Through the cooperation of the lever principle and the spring, the precise insertion and extraction of the piercing hollow needle is achieved to avoid damage to other tissues.
The device can accurately sample and freeze tumor tissue without destroying other tissues of the tumor organoids, reduce resource waste, simplify operational steps, and improve work efficiency.
Smart Images
Figure CN222935404U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of medical devices and relates to a sampling device for culturing tumor organoids. Background Art
[0002] At present, various tumor diseases have become health killers of people. An organoid refers to an aggregate or cluster of cells composed of multiple cells and having biological activity, belonging to three-dimensional cell cultures. Compared with single cells, organoids are closer to the actual situation of multiple cells growing in cooperation.
[0003] At present, organoid culture has been used in various tissues, including the intestine, liver, pancreas, kidney, prostate, lung, optic cup, and brain, etc. As a tool, organoid technology has great potential in both scientific research and clinical applications, including developmental biology, cell biology, regeneration mechanisms, precision medicine, and drug toxicity and efficacy tests. Compared with single-cultured cells, a smaller microenvironment can be established among the cells in an organoid, and there is effective cell communication. The cells in an organoid can enable surrounding cells to receive chemical signals and make corresponding responses through the paracrine pathway of cytokines. Some organoids contain differentiation potential, which is of great significance for studying the development of tissues and organs and the regulation of related signaling molecules.
[0004] Tumor organoid models can well predict the drug sensitivity of in-situ tumors. Therefore, the drug sensitivity of in-situ tumors can be tested by culturing tumor organoids. Culturing tumor organoids requires relevant tumor tissues, which requires separating the internal structure from the tumor tissues. However, the current devices and methods for separating the internal structure from tumor tissues are prone to damaging the entire tumor tissue, resulting in the inability to use other parts of the tumor tissue and causing waste of resources. Summary of the Utility Model
[0005] The purpose of the utility model is to provide a sampling device for culturing tumor organoids to solve the problem that the existing device is prone to damaging the entire tumor tissue when separating the internal structure from the tumor tissue.
[0006] To achieve the above purpose, the utility model adopts the following technical solutions:
[0007] The utility model provides a sampling device for culturing tumor organoids, including a base, a bracket located on one side of the base, and a sleeve arranged on the bracket; wherein,
[0008] A syringe barrel and a needle holder elastically connected by a stamping spring are arranged inside the sleeve. A push rod and a sliding rod are arranged on the outer side of the syringe barrel, and the syringe barrel and the needle holder move up and down in the sleeve; a piercing hollow needle is arranged on the needle holder, and the piercing hollow needle is located inside the syringe barrel;
[0009] The bracket is provided with a linkage pull rod and a return spring. Two ends of the return spring are respectively connected to the bracket and an end of the linkage pull rod; the top end of the linkage pull rod is movably clamped to the needle holder; the ejector rod abuts against the linkage pull rod;
[0010] A hand pressure rod is rotatably arranged on the outer side of the bracket, and the sliding rod passes through the sleeve and is slidably arranged in the hand pressure rod.
[0011] Preferably, a cutting and pressing head is arranged at an end of the piercing hollow needle, and the cutting and pressing head is a three-cone-surface cutting and pressing head.
[0012] Preferably, a taper angle of a conical surface of the cutting and pressing head is 20°.
[0013] Preferably, a semi-permeable membrane support body is arranged outside the piercing hollow needle.
[0014] Preferably, a limiting plate is further arranged inside the sleeve. The limiting plate is sleeved on the piercing hollow needle and is located inside the needle cylinder.
[0015] Preferably, 2-6 piercing hollow needles are arranged on the needle holder.
[0016] Preferably, a length of the piercing hollow needle is ≥90 mm.
[0017] Preferably, a container is further arranged on the base, and the container is located below the sleeve.
[0018] Preferably, a bottom end of the linkage pull rod is movably hinged inside the bracket.
[0019] Preferably, the hand pressure rod is a U-shaped hand pressure rod, and the sliding rod is located on a U-shaped arm of the hand pressure rod.
[0020] The utility model has the following beneficial effects:
[0021] (1) Through the cooperative action of the hand pressure rod, the needle cylinder, the linkage pull rod, the punching spring and the return spring, etc., the piercing hollow needle can be inserted into the tumor-like organ by using the lever principle without damaging other tissues of the tumor-like organ in the present application.
[0022] (2) After sampling the tumor-like organ by the piercing hollow needle in the present application, the semi-permeable membrane support body outside the piercing hollow needle will remain in the tumor-like organ so that the cryopreservation solution can better preserve the tumor-like organ.
[0023] (3) In the present application, the size of the container can be replaced according to the size and shape of the tumor-like organ, and the descending amplitude of the piercing hollow needle can be arbitrarily changed by the rotation angle of the hand pressure rod, and the applicable range of tumor tissues is wide.
[0024] (4)The sampling device has a simple structure, perfect functions, and is convenient to use. It causes little damage to the whole excised tumor tissue, has accurate positioning, is convenient and fast, simplifies the operation steps, improves work efficiency, and reduces the work intensity of operators. Description of the Drawings
[0025] Figure 1 It is an exploded perspective view of the sampling device for culturing tumor organoids provided by an embodiment of the present application;
[0026] Figure 2 It is a perspective view of the sampling device for culturing tumor organoids provided by an embodiment of the present application;
[0027] Figure 3 It is a cross-sectional view of the sampling device for culturing tumor organoids provided by an embodiment of the present application;
[0028] Figure 4 It is an enlarged structural schematic diagram of the cutting pressure head at the piercing hollow needle provided by an embodiment of the present application;
[0029] Figure 5 It is a usage state diagram of the sampling device for culturing tumor organoids provided by an embodiment of the present application;
[0030] Symbol representation:
[0031] 1 - Base, 2 - Bracket, 3 - Sleeve, 4 - Stamping spring, 5 - Syringe barrel, 6 - Needle holder, 7 - Ejector rod, 8 - Sliding rod, 9 - Piercing hollow needle, 10 - Linkage pull rod, 11 - Return spring, 12 - Hand pressure rod, 13 - Cutting pressure head, 14 - Limiting plate, 15 - Container, 16 - Chute, 17 - Through groove, 18 - Boss. Detailed Embodiments
[0032] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0033] An embodiment of the present application provides a sampling device for culturing tumor organoids. The device includes a base 1, a bracket 2 located on one side of the base 1, and a sleeve 3 provided on the bracket 2. As shown in the attached Figures 1-3As shown in the figure. Among them, the base 1 is a plate structure, which is used to set up the bracket and place the container 15, and the tumor organoids are placed in the container 15. The bracket 2 is a supporting component, which is used to set up the sleeve 3. The sleeve 3 is a component for setting up the piercing hollow needle 9, the hand pressure rod 12, etc., and it is fixed on the bracket 2. The container 15 is located below the sleeve 3 so that the piercing hollow needle 9 can sample from the tumor organoids placed in the container 15. The size of the container 15 can be adjusted according to the size and shape of the tumor organoids.
[0034] Specifically, a syringe barrel 5 and a needle holder 6 are arranged inside the sleeve 3. The top of the syringe barrel 5 and the top of the needle holder 6 are elastically connected by a stamping spring 4, and the syringe barrel 5 and the needle holder 6 move up and down inside the sleeve 3. A push rod 7 and a sliding rod 8 are arranged on the outer side of the syringe barrel 5. Among them, the push rod 7 is used to push the linkage lever 10, and the sliding rod 8 is used to drive the sleeve 3 to move up and down by the hand pressure rod 12. A piercing hollow needle 9 is arranged on the needle holder 6, and the piercing hollow needle 9 is located inside the syringe barrel 5. As the needle holder 6 moves up and down inside the sleeve 3, the piercing hollow needle 9 also moves up and down inside the sleeve 3.
[0035] In the embodiment of the present application, a cutting and pressing head 13 is arranged at the end of the piercing hollow needle 9, and the cutting and pressing head 13 is a three-cone surface cutting and pressing head, as shown in the appendix Figure 4 As shown. When the piercing hollow needle 9 moves downward to the tumor organoids placed in the container 15, the cutting and pressing head 13 in the form of a three-cone surface cutting and pressing head can easily insert into the tumor organoids, thereby facilitating the extraction of tissues in the tumor organoids. Further, the cone angle of the cutting and pressing head 13 is 20°, and the cutting and pressing head 13 at this angle is more likely to enter the tumor organoids and is not likely to damage other tissues of the tumor organoids.
[0036] In addition, 2-6 piercing hollow needles 9 are arranged on the needle holder 6, and the length of the piercing hollow needle 9 ≥ 90 mm. Preferably, 4 piercing hollow needles 9 are arranged on the needle holder 6. A semi-permeable membrane support (not shown in the figure) is arranged outside the piercing hollow needle 9. When the piercing hollow needle 9 is inserted into the tumor organoids, the semi-permeable membrane support will stay inside the tumor organoids and play a role in supporting the tumor organoids. At this time, the piercing hollow needle 9 has withdrawn, and the cryopreservation solution can enter the tumor organoids through the space supported by the semi-permeable membrane support, and the tumor organoids will not collapse due to the removal of some tissues, which is ultimately beneficial for cryopreservation in liquid nitrogen.
[0037] Inside the bracket 2 of the embodiment of the present application, there is a linkage pull rod 10 and a return spring 11. Among them, both ends of the return spring 11 are respectively connected to the bracket 2 and the top end of the linkage pull rod 10. In addition, the bottom end of the linkage pull rod 10 is movably hinged inside the bracket 2. Thus, under the elastic action of the return spring 11, the top end of the linkage pull rod 10 can rotate around the hinged part at the bottom end. The ejector rod 7 outside the syringe barrel 5 abuts against the linkage pull rod 10. Thus, when the syringe barrel 5 moves downward in the sleeve 3, it can push the linkage pull rod 10 to rotate towards the return spring 11 side, thereby compressing the return spring 11. When the syringe barrel 5 moves upward in the sleeve 3, under the elastic action of the return spring 11, the linkage pull rod 10 rotates towards the syringe barrel 5 side to realize the reset of the linkage pull rod 10.
[0038] In addition, the top end of the linkage pull rod 10 is movably clamped with the needle holder 6. Specifically, both the needle holder 6 and the top end of the linkage pull rod 10 are inclined planes with a certain slope, and the slopes of the two slopes are the same. When the sampling device is not in use, the end of the linkage pull rod 10 is located below the needle holder 6 and is clamped between the side wall and the top end of the needle holder 6. When the sampling device is in use, the syringe barrel 5 moves downward, and the ejector rod 7 outside the syringe barrel 5 pushes the linkage pull rod 10 to rotate towards the return spring 11 side and compresses the return spring 11. When the top of the linkage pull rod 10 disengages from the top of the syringe barrel 5, that is, the linkage pull rod 10 cannot hold the syringe barrel 5, at this time, under the action of the stamping spring 4, the needle holder 6 is also pulled downward by the syringe barrel 5, and then the hollow needle 9 is pierced and inserted downward into the tumor organ, as shown in the attachment. Figure 5 When the sampling with the hollow needle 9 is completed, the syringe barrel 5 moves upward. Under the pushing action of the stamping spring 4, the needle holder 6 also moves upward. At this time, the ejector rod 7 outside the syringe barrel 5 no longer pushes the linkage pull rod 10. Under the elastic action of the return spring 11, the linkage pull rod 10 rotates towards the syringe barrel 5 side until the linkage pull rod 10 is clamped below the top of the syringe barrel 5 to realize the reset of the syringe barrel 5, the needle holder 6 and the linkage pull rod 10.
[0039] In the embodiment of the present application, to facilitate the control of the up and down movement of the syringe barrel 5, a sliding rod 8 is provided on the outer side of the syringe barrel 5, and a hand pressure rod 12 is rotatably arranged on the outer side of the bracket 2. The sliding rod 8 passes through the sleeve 3 and is slidably arranged in the hand pressure rod 12. Specifically, a chute 16 is provided on the sleeve 3. After the sliding rod 8 passes through the chute 16, it is exposed outside the sleeve 3. One end of the hand pressure rod 12 is movably hinged to the bracket 2, and the other end can rotate along the hinged part. A through groove 17 is provided on the hand pressure rod 12, and the sliding rod 8 exposed outside the sleeve 3 is located in the through groove 17. When the hand pressure rod 12 rotates upward or downward around the end located on the bracket 2, the sliding rod 8 can be driven to move up and down through the through groove 17, and finally the syringe barrel 5 is driven to move up and down to realize the descent of the hollow needle 9 for piercing. The descent amplitude of the hollow needle 9 for piercing in the embodiment of the present application can be arbitrarily changed by the rotation angle of the hand pressure rod 12.
[0040] Furthermore, the hand pressure rod 12 in the embodiment of the present application is a U-shaped hand pressure rod, and the through groove 17 is provided on the U-shaped arm of the hand pressure rod 12 , so that the sliding rod 8 is located in the through groove 17 .
[0041] In order to limit the downward position of the syringe 5, a boss 18 is provided on the linkage pull rod 10. When the push rod 7 moves downward to the boss 18, the push rod 7 is stuck on the boss 18, and the syringe 5 cannot move downward further, thereby preventing the syringe 5 from being completely exposed outside the sleeve 3. In addition, in order to limit the downward position of the piercing hollow needle 9, a limiting plate 14 is further provided inside the sleeve 3, which is sleeved on the piercing hollow needle 9 and located inside the syringe 5.
[0042] The process of tissue sampling and tissue cryopreservation using the sampling device for tumor organoid culture provided in the embodiment of the present application is as follows:
[0043] (1) Soak the oval tumor tissue of cancer or tumor patients in tissue preservation fluid to ensure tissue activity.
[0044] (2) The tumor tissue is taken out from the tissue preservation solution, placed in the container 15, and the container 15 is placed under the sleeve 3.
[0045] (3) Press the hand lever 12 downward, and the sliding rod 8 in the through groove 17 moves downward along the sliding groove 16, thereby driving the syringe 5 to move downward. During the downward movement of the syringe 5, the push rod 7 outside the syringe 5 pushes the linkage lever 10 to rotate toward the return spring 11 and compresses the return spring 11. When the top of the linkage lever 10 is separated from the top of the syringe 5, under the action of the punch spring 4, the needle holder 6 is pulled by the syringe 5 and also moves downward, thereby piercing the hollow needle 9 and inserting it downward into the tumor tissue.
[0046] (4) Lift the hand pressure rod 12 upward, and the syringe 5 moves upward. Under the push of the punch spring 4, the needle holder 6 also moves upward. At this time, the push rod 7 outside the syringe 5 no longer pushes the linkage pull rod 10. Under the elastic action of the reset spring 11, the linkage pull rod 10 rotates toward the syringe 5 until the linkage pull rod 10 is stuck under the top of the syringe 5, realizing the reset of the syringe 5, the needle holder 6 and the linkage pull rod 10. At this time, the piercing hollow needle 9 leaves the tumor tissue, and during the process of leaving, the semipermeable membrane support body located outside the piercing hollow needle 9 remains in the tumor tissue and expands the inside of the tumor tissue.
[0047] (5) The expanded tumor tissue is quickly placed in cryopreservation solution, cooled gradually using a programmable cooling device, and finally frozen in liquid nitrogen.
[0048] The above are only the preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, various changes and modifications can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included within the protection scope of the present application.
Claims
1. A sampling device for tumor organoid culture, characterized in that: It comprises a base (1), a bracket (2) located on one side of the base (1), and a sleeve (3) arranged on the bracket (2); wherein: A syringe (5) and a needle holder (6) elastically connected via a punching spring (4) are provided inside the sleeve (3); a push rod (7) and a sliding rod (8) are provided outside the syringe (5); and the syringe (5) and the needle holder (6) move up and down inside the sleeve (3); a piercing hollow needle (9) is provided on the needle holder (6), and the piercing hollow needle (9) is located inside the syringe (5); A linkage rod (10) and a return spring (11) are provided in the bracket (2), and two ends of the return spring (11) are respectively connected to the bracket (2) and the end of the linkage rod (10); the top end of the linkage rod (10) is movably engaged with the needle holder (6); and the push rod (7) abuts against the linkage rod (10); A hand pressure rod (12) is rotatably arranged on the outer side of the bracket (2), and the sliding rod (8) passes through the sleeve (3) and is slidably arranged in the hand pressure rod (12).
2. The sampling device for tumor organoid culture according to claim 1, characterized in that: The end of the piercing hollow needle (9) is provided with a cutting head (13), and the cutting head (13) is a three-cone cutting head.
3. The sampling device for tumor organoid culture according to claim 2, characterized in that: The cone angle of the cutting and pressing head (13) is 20°.
4. The sampling device for tumor organoid culture according to claim 1, characterized in that: A semipermeable membrane support is provided outside the piercing hollow needle (9).
5. The sampling device for tumor organoid culture according to claim 1, characterized in that: A limiting plate (14) is also provided inside the sleeve (3), and the limiting plate (14) is sleeved on the piercing hollow needle (9) and is located inside the syringe (5).
6. The sampling device for tumor organoid culture according to claim 1, characterized in that: The needle holder (6) is provided with 2 to 6 hollow piercing needles (9).
7. The sampling device for tumor organoid culture according to claim 1, characterized in that: The length of the piercing hollow needle (9) is ≥ 90 mm.
8. The sampling device for tumor organoid culture according to claim 1, characterized in that: The base (1) is also provided with a container (15), and the container (15) is located below the sleeve (3).
9. The sampling device for tumor organoid culture according to claim 1, characterized in that: The bottom end of the linkage pull rod (10) is movably hinged inside the bracket (2).
10. The sampling device for tumor organoid culture according to claim 1, characterized in that: The hand pressure rod (12) is a U-shaped hand pressure rod, and the sliding rod (8) is located on the U-shaped arm of the hand pressure rod (12).