Exosome extraction device

Abstract

The utility model relates to the technical field of exosome extraction, and discloses an exosome extraction device which comprises a base, a side plate is fixedly connected to the base, a connecting rod is movably connected to the side plate, and a gear is fixedly installed on the outer side of the connecting rod. The motor works to drive the screw rod to rotate, and the push plate can be driven to move and adjust back and forth through the threads, so that the teeth drive the gear to rotate forwards and reversely, the connecting rod can drive the extraction box to shake back and forth, and liquid fed into the extraction box can shake back and forth at the first filter screen firstly to be filtered; impurities are removed through filtering, and the form of back-and-forth shaking filtering is adopted, so that the impurities can shake back and forth along with the liquid in the filtering process, the situation that the impurities block the filter screens in a large area can be avoided, and the extraction efficiency is not affected is avoided; the exosomes are separated and extracted successively, so that the extracted exosomes are purer.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome extraction, and more specifically, to an exosome extraction device. Background Art

[0002] Exosomes refer to small membrane vesicles of 30-150nm containing complex RNA and proteins. Nowadays, they specifically refer to disc-shaped vesicles with a diameter of 40-100nm. All cultured cell types can secrete exosomes, which exist naturally in body fluids, including blood, saliva, and urine. Nowadays, research on exosomes is becoming more and more frequent. In research, exosomes need to be obtained through exosome extraction devices. The common method for separating exosomes is filtration, but exosomes are small in size and the filter mesh used is small. After multiple filtrations, it is very easy to cause blockage, which is not conducive to the extraction of exosomes. Utility Model Content

[0003] In order to overcome the deficiencies of the prior art, the utility model provides an exosome extraction device, which has the advantage of avoiding large-area blockage affecting filtration.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an exosome extraction device, comprising a base, a side plate fixedly connected to the base, a connecting rod movably connected to the side plate, a gear fixedly installed on the outer side of the connecting rod, an extraction box fixedly connected to the inner side of the connecting rod, an upper cover installed on the upper end of the extraction box, a feeding pipe fixedly connected to the top of the upper cover, a feeding pipe fixedly connected to the bottom of the extraction box, an installation groove is provided inside the extraction box, a first filter screen, a second filter screen and a third filter screen are installed in sequence inside the extraction box from top to bottom, a motor is fixedly installed on the top of the base, a screw is fixedly connected to the motor, and a push plate is threadedly connected to the outer side of the screw.

[0005] As a preferred technical solution of the utility model, a slide groove is provided on the top of the base, a slider sliding along the inside of the slide groove is fixedly installed on the bottom of the push plate, and teeth meshing with the gear are fixedly installed at equal distances on the top of the push plate.

[0006] As a preferred technical solution of the utility model, a tube cover is installed on the feeding tube, a sealing cover is installed on the discharge tube, and the bottom inside the extraction box is a funnel-shaped structure.

[0007] As a preferred technical solution of the utility model, both sides of the upper end of the extraction box are fixedly connected with limiting plates, the upper end of the limiting plate is provided with a second screw hole, both ends of the upper cover are provided with a first screw hole, and the internal thread of the second screw hole is connected with a bolt that can be inserted into the first screw hole.

[0008] As a preferred technical solution of the utility model, the mesh aperture values ​​of the first filter screen, the second filter screen and the third filter screen decrease successively, and the outer sides of the first filter screen, the second filter screen and the third filter screen are fixedly installed with installation frames, and a connecting plate is fixedly connected between every two adjacent installation frames, and the top of the installation frame is a slope structure.

[0009] As a preferred technical solution of the utility model, handles are fixedly connected to both sides of the upper cover, and a pressing plate that can be inserted into the installation groove is fixedly connected to the bottom of the upper cover, and the bottom of the pressing plate is a slope structure.

[0010] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0011] The utility model drives the screw to rotate through the operation of the motor, and drives the push plate to move back and forth for adjustment by using the thread, so that the gears are driven to rotate in the forward and reverse directions by using the teeth, and the extraction box is driven to swing back and forth by the connecting rod. Then, the liquid put into the extraction box will first be shaken back and forth and filtered at the first filter screen, and then flow into the second filter screen to shake back and forth and filter, and then flow into the third filter screen to shake back and forth and filter, and impurities are removed by filtering. The back and forth shaking filtration method can make the impurities swing back and forth with the liquid during the filtration process, so as to avoid large-scale clogging of the filter screen by impurities, avoid affecting the extraction efficiency, and separate and extract the exosomes one by one, so that the extracted exosomes are purer. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0013] Figure 2 This is a schematic diagram of the internal cross-sectional structure of the extraction box of the utility model;

[0014] Figure 3 This is a schematic diagram of the utility model when the first filter screen, the second filter screen and the third filter screen are taken out.

[0015] In the figure: 1. base; 101. slide; 2. side panel; 3. extraction box; 4. upper cover; 401. handle; 402. pressure plate; 403. first screw hole; 5. motor; 6. screw; 7. push plate; 701. slider; 8. connecting rod; 9. gear; 10. feeding pipe; 1001. pipe cover; 11. limit plate; 111. second screw hole; 12. bolt; 13. feeding pipe; 131. sealing cover; 14. first filter; 15. second filter; 16. third filter; 17. mounting frame; 18. connecting plate; 19. mounting groove. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0017] like Figures 1 to 3 As shown, the utility model provides an exosome extraction device, including a base 1, a side plate 2 is fixedly connected to the base 1, a connecting rod 8 is movably connected to the side plate 2, a gear 9 is fixedly installed on the outer side of the connecting rod 8, an extraction box 3 is fixedly connected to the inner side of the connecting rod 8, an upper cover 4 is installed on the upper end of the extraction box 3, a feeding pipe 10 is fixedly connected to the top of the upper cover 4, a feeding pipe 13 is fixedly connected to the bottom of the extraction box 3, a mounting groove 19 is opened inside the extraction box 3, a first filter screen 14, a second filter screen 15 and a third filter screen 16 are sequentially installed inside the extraction box 3 from top to bottom, a motor 5 is fixedly installed on the top of the base 1, a screw 6 is fixedly connected to the motor 5, and the external thread of the screw 6 A push plate 7 is connected; the screw rod 6 is driven to rotate by the motor 5, and the push plate 7 can be driven to move back and forth for adjustment by the thread, so that the gear 9 is driven to rotate forward and reverse by the teeth, and the extraction box 3 can be driven to swing back and forth by the connecting rod 8. Then, the liquid put into the extraction box 3 will first be shaken back and forth and filtered at the first filter screen 14, and then flow into the second filter screen 15 to shake back and forth and filter, and then flow into the third filter screen 16 to shake back and forth and filter, and impurities are removed by filtering. In addition, the back and forth shaking filtration method is adopted, so that the impurities will shake back and forth with the liquid during the filtration process, which can avoid large-scale clogging of the filter screen by impurities and avoid affecting the extraction efficiency. By separating and extracting the exosomes one by one, the extracted exosomes are purer.

[0018] The top of the base 1 is provided with a slide groove 101, the bottom of the push plate 7 is fixedly provided with a slider 701 that slides along the inside of the slide groove 101, and the top of the push plate 7 is fixedly provided with teeth that mesh with the gear 9 at equal intervals;

[0019] By sliding the slider 701 along the inside of the slide groove 101, the push plate 7 can be made more stable during the back and forth movement. At the same time, the push plate 7 that moves back and forth can drive the gear 9 meshing with it to rotate forward and reverse through the teeth, so as to ensure that the extraction box 3 can be stably driven to swing back and forth through the connecting rod 8, which is beneficial to the extraction of exosomes.

[0020] Among them, a tube cover 1001 is installed on the feeding tube 10, a sealing cover 131 is installed on the feeding tube 13, and the bottom of the extraction box 3 is a funnel-shaped structure;

[0021] The liquid to be extracted can be fed into the extraction box 3 through the feeding pipe 10, and the feeding pipe 10 can be closed through the tube cover 1001 to prevent the liquid from spilling out when shaking; by removing the sealing cover 131, the exosomes extracted in the extraction box 3 can be discharged through the discharge pipe 13 for collection, and the bottom of the extraction box 3 is designed as a funnel-shaped structure, which is conducive to the collection and discharge of the filtered exosomes.

[0022] The two sides of the upper end of the extraction box 3 are fixedly connected with the limit plate 11, the upper end of the limit plate 11 is provided with a second screw hole 111, and the two ends of the upper cover 4 are provided with a first screw hole 403, and the inner thread of the second screw hole 111 is connected with a bolt 12 that can be inserted into the first screw hole 403;

[0023] By screwing the bolt 12 through the second screw hole 111 and inserting it into the first screw hole 403, the extraction box 3 and the upper cover 4 can be connected together, making it difficult for the upper cover 4 to separate from the extraction box 3. At the same time, by removing the bolt 12 and then dismantling the upper cover 4, the first filter 14, the second filter 15 and the third filter 16 can be easily taken out for cleaning.

[0024] The mesh aperture values ​​of the first filter screen 14, the second filter screen 15 and the third filter screen 16 decrease in sequence. The outer sides of the first filter screen 14, the second filter screen 15 and the third filter screen 16 are fixedly installed with a mounting frame 17. A connecting plate 18 is fixedly connected between every two adjacent mounting frames 17. The top of the mounting frame 17 is a slope structure.

[0025] By reducing the mesh aperture values ​​of the first filter 14, the second filter 15 and the third filter 16 in sequence, the exosomes can be separated and extracted one by one, and the extracted exosomes are purer; the installation frame 17 is arranged and placed inside the installation groove 19, which is convenient for the installation of the first filter 14, the second filter 15 and the third filter 16. The connection plate 18 is arranged to make the first filter 14, the second filter 15 and the third filter 16 become a whole, which is convenient for installation and disassembly.

[0026] Among them, handles 401 are fixedly connected to both sides of the upper cover 4, and a pressing plate 402 that can be inserted into the installation groove 19 is fixedly connected to the bottom of the upper cover 4, and the bottom of the pressing plate 402 is a slope structure;

[0027] The handles 401 at both ends make it easy to manually lift the upper cover 4 for disassembly and assembly. The pressing plate 402 is inserted into the installation groove 19 and the slope structure at the bottom is abutted against the slope structure at the top of the installation frame 17 outside the first filter screen 14, which can be used to press and fix it to prevent movement when shaking.

[0028] The working principle and use process of this utility model:

[0029] First, liquid can be added into the extraction box 3 through the feeding pipe 10, and then covered with the pipe cover 1001;

[0030] Then, the motor 5 is started to drive the screw 6 to rotate, and the push plate 7 can be driven to move back and forth by the thread, so that the gear 9 can be driven to rotate forward and reverse by the teeth, and the extraction box 3 can be driven to swing back and forth by the connecting rod 8. Then, the liquid put into the extraction box 3 will first be shaken back and forth and filtered at the first filter screen 14, and then flow into the second filter screen 15 to shake back and forth and filter, and then flow into the third filter screen 16 to shake back and forth and filter. By separating and extracting one by one and filtering to remove impurities, the exosome liquid will flow into the lower end of the extraction box 3.

[0031] After the filtration is completed, the sealing cover 131 can be opened to allow the exosome liquid to be discharged and collected through the discharge pipe 13 .

[0032] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0033] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An exosome extraction device, comprising a base (1), characterized in that: The base (1) is fixedly connected to a side plate (2), the side plate (2) is movably connected to a connecting rod (8), a gear (9) is fixedly installed on the outer side of the connecting rod (8), an extraction box (3) is fixedly connected on the inner side of the connecting rod (8), an upper cover (4) is installed on the upper end of the extraction box (3), a feeding pipe (10) is fixedly connected to the top of the upper cover (4), a discharge pipe (13) is fixedly connected to the bottom of the extraction box (3), a mounting groove (19) is provided inside the extraction box (3), a first filter screen (14), a second filter screen (15) and a third filter screen (16) are installed in sequence from top to bottom inside the extraction box (3), a motor (5) is fixedly installed on the top of the base (1), a screw rod (6) is fixedly connected to the motor (5), and a push plate (7) is threadedly connected to the outer side of the screw rod (6).

2. An exosome extraction device according to claim 1, characterized in that: A slide groove (101) is provided on the top of the base (1), a slider (701) that slides along the inside of the slide groove (101) is fixedly installed on the bottom of the push plate (7), and teeth that mesh with the gear (9) are fixedly installed at equal intervals on the top of the push plate (7).

3. The exosome extraction device according to claim 1, characterized in that: The feeding pipe (10) is provided with a pipe cover (1001), the feeding pipe (13) is provided with a sealing cover (131), and the bottom of the extraction box (3) is a funnel-shaped structure.

4. The exosome extraction device according to claim 1, characterized in that: Both sides of the upper end of the extraction box (3) are fixedly connected to a limit plate (11), and a second screw hole (111) is provided at the upper end of the limit plate (11). Both ends of the upper cover (4) are provided with a first screw hole (403), and the internal thread of the second screw hole (111) is connected to a bolt (12) that can be inserted into the first screw hole (403).

5. The exosome extraction device according to claim 1, characterized in that: The mesh aperture values ​​of the first filter screen (14), the second filter screen (15) and the third filter screen (16) decrease in sequence; a mounting frame (17) is fixedly mounted on the outer sides of the first filter screen (14), the second filter screen (15) and the third filter screen (16); a connecting plate (18) is fixedly connected between every two adjacent mounting frames (17); and the top of the mounting frame (17) is a slope structure.

6. The exosome extraction device according to claim 1, characterized in that: Handles (401) are fixedly connected to both sides of the upper cover (4), and a pressing plate (402) capable of being inserted into the installation groove (19) is fixedly connected to the bottom of the upper cover (4), and the bottom of the pressing plate (402) is a slope structure.

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