Full-automatic collection device for freshwater fish eDNA samples

Abstract

The utility model discloses a full-automatic collection device for freshwater fish eDNA samples, and belongs to the technical field of fish eDNA sample collection. The device comprises a supporting assembly, wherein the supporting assembly comprises a bottom plate; the collecting assembly comprises three clamping blocks installed above the bottom plate, a first telescopic rod is installed at the end, away from the bottom plate, of any clamping block, a first sliding cylinder is installed on the first telescopic rod, and a first spring is installed in the first sliding cylinder; according to the full-automatic collection device for the freshwater fish eDNA samples, by arranging the collection assembly and the conveying assembly, fishes can be directly placed on the conveying belt, then DNA collection is directly conducted on fish clamping columns on the conveying belt through clamping blocks, the efficiency is extremely high, much cost can be saved, errors are small, and the collection amount of each time is basically consistent; meanwhile, through the arranged cleaning pool, the collected needle heads can be rapidly cleaned.

Description

Technical Field

[0001] The utility model relates to the technical field of fish eDNA sample collection, in particular to a fully automatic collection device for freshwater fish eDNA samples. Background Art

[0002] There are several important reasons to study environmental DNA (eDNA) of fish: 1. Non-invasive sampling of freshwater fish: eDNA analysis of freshwater fish can detect the presence of specific fish through DNA analysis in environmental samples such as water samples, without capturing or disturbing the target species, so it has less impact on the environment. 2. Improved detection efficiency of freshwater fish: Compared with traditional survey methods such as fishing or visual observation, eDNA technology can often detect the presence of target species more quickly and accurately, especially in rare or difficult-to-observe environments. 3. Ecological monitoring of freshwater fish: eDNA analysis of freshwater fish can help scientists monitor the distribution, number and ecological role of fish, which is very useful for protecting and managing aquatic ecosystems. 4. Monitoring of invasive species in freshwater fish: Freshwater fish can use eDNA technology to timely monitor and identify invasive alien species, which helps to take early control measures to protect local biodiversity. 5. Environmental health assessment of freshwater fish: eDNA analysis of freshwater fish can be used as a tool to assess the health of water bodies and ecosystems, helping to discover and solve environmental problems. In short, by studying eDNA of fish, scientists can more fully understand the ecology and protection needs of aquatic organisms, providing important support for sustainable management and protection of aquatic resources.

[0003] The existing collection methods are all done manually, but the labor cost is high: manual collection requires a lot of manpower input, and more staff may be required during large-scale sample collection, increasing costs and workload; human errors: due to the operator's subjective factors, data errors may exist during manual collection, including sample labeling errors, inconsistent sampling and other problems; time-consuming and labor-intensive: manual collection requires a lot of time and energy, especially in complex or large-scale collection tasks, the workload is large.

[0004] Therefore, a new fully automatic collection device for freshwater fish eDNA samples is proposed to solve the above problems. Utility Model Content

[0005] The utility model aims to provide a fully automatic collection device for freshwater fish eDNA samples to solve the technical problems raised in the background technology.

[0006] In order to solve the above technical problems, the utility model is realized by the following technical solutions:

[0007] The utility model is a fully automatic collection device for freshwater fish eDNA samples, comprising a support component, wherein the support component comprises a bottom plate;

[0008] A collection component, the collection component includes three clamping blocks installed above the bottom plate, any one of the clamping blocks is installed with a first telescopic rod at one end away from the bottom plate, a first sliding cylinder is installed on the first telescopic rod, a first spring is installed in the first sliding cylinder, an end of the first spring close to the bottom plate is connected with an end of the first telescopic rod close to the bottom plate, a second connecting rod is installed at one end of the first sliding cylinder, a second sliding cylinder is installed at one end of the second connecting rod close to the bottom plate, a second telescopic rod is installed in the second sliding cylinder, a second spring is installed in the second sliding cylinder, an end of the second spring close to the bottom plate is connected with an end of the second telescopic tube close to the bottom plate, a pressing rod is installed at one end of the second connecting rod close to the first sliding cylinder, a third sliding cylinder is installed at one end of the pressing rod close to the bottom plate, an air groove is installed in the third sliding cylinder, an end of the pressing rod close to the bottom plate is installed in the air groove, and a needle is installed at one end of the third sliding cylinder close to the bottom plate;

[0009] The conveying assembly comprises four first brackets installed on one end of the bottom plate close to the clamping block, and conveying belts are installed on the four brackets, and the conveying belts are located directly below the clamping block.

[0010] Preferably, a gas rod is installed at one end of the pressing rod away from the bottom plate, and a gas cylinder is installed at one end of the gas rod away from the pressing rod.

[0011] Preferably, the needle is connected to the air groove, and four connecting holes are provided on the needle, and any one of the connecting holes is connected to the needle hole of the pillow itself.

[0012] Preferably, a rotating disk is installed at one end of the three cylinders away from the base plate, a transmission shaft is installed at one end of the rotating disk away from the base plate, a motor is installed at one end of the transmission shaft away from the base plate, a right-angle support frame is installed at one end of the motor away from the base plate, and the lowermost end of the right-angle support frame is connected to one end of the base plate close to the motor.

[0013] Preferably, a cleaning box is installed at one end of the base plate close to the right-angle support frame.

[0014] Preferably, four second brackets are installed at one end of the bottom plate close to the cleaning box, a workbench is installed at one end of the four second brackets away from the bottom plate, and a collecting cylinder is installed at one end of the workbench away from the second brackets.

[0015] Compared with the prior art, the advantages of the present invention are:

[0016] The fully automatic eDNA sample collection device for freshwater fishes can directly place the fishes on the conveyor belt through the provided collection components and conveying components, and then the clamping block directly uses the fish clamping column on the conveyor belt to collect DNA, which is highly efficient and can save a lot of costs, and has a small error, and the collection amount each time is basically the same; at the same time, the collected needles can be quickly cleaned through the provided cleaning pool.

[0017] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 This is the installation structure diagram of the utility model;

[0020] Figure 2 This is a structural diagram of the acquisition component of the utility model;

[0021] Figure 3 This is a cross-sectional structural diagram of the collection component of the utility model;

[0022] Figure 4 For the utility model Figure 3 Enlarged view of point A in the middle;

[0023] Figure 5 This is a structural diagram of the cylinder and gas rod of the utility model;

[0024] Figure 6 This is a structural diagram of the conveying component of the utility model;

[0025] Figure 7 This is a structural diagram of the motor and the rotating disk of the utility model;

[0026] Figure 8 This is a structural diagram of a workbench and a cleaning box of the utility model.

[0027] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0028] 100, collecting component; 110, clamping block; 120, first telescopic rod; 121, first sliding cylinder; 122, first spring; 130, second sliding cylinder; 131, second telescopic rod; 132, second spring; 140, pressing rod; 141, third sliding cylinder; 142, needle; 143, connecting hole; 150, first connecting rod; 160, second connecting rod; 170, cylinder; 171, gas rod; 200, conveying component; 210, conveyor belt; 220, first bracket; 300, supporting component; 310, bottom plate; 320, right-angle supporting frame; 330, motor; 340, transmission shaft; 350, rotating disk; 360, cleaning box; 370, second bracket; 380, workbench; 390, collecting cylinder. DETAILED DESCRIPTION

[0029] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the embodiments of the present invention. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0030] In the description of the embodiments of the present invention, it should be understood that the terms "length", "vertical", "horizontal", "top", "bottom", etc. indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present invention.

[0031] The relative importance is implied or the number of technical features indicated is implicitly indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the embodiments of the present utility model, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0032] In order to better understand the purpose, structure and function of the utility model, the following is a further detailed description of the utility model of a fully automatic collection device for freshwater fish eDNA samples in conjunction with the accompanying drawings.

[0033] See also Figure 1-8As shown, this embodiment is a fully automatic collection device for freshwater fish eDNA samples, including a support component 300, the support component 300 includes a bottom plate 310; a collection component 100, the collection component 100 includes three clamping blocks 110 installed above the bottom plate 310, any one of the clamping blocks 110 is installed with a first telescopic rod 120 at one end away from the bottom plate 310, the first telescopic rod 120 is installed with a first sliding cylinder 121, the first sliding cylinder 121 is installed with a first spring 122, the end of the first spring 122 close to the bottom plate 310 is connected to the end of the first telescopic rod 120 away from the bottom plate 310, the first sliding cylinder 121 is installed with a second connecting rod 160 at one end, and the second connecting rod 160 is installed at one end. The second sliding cylinder 130 is installed at one end of the rod 160 close to the bottom plate 310, and the second telescopic rod 131 is installed in the second sliding cylinder 130. The second spring 132 is installed in the second sliding cylinder 130. The end of the second spring 132 close to the bottom plate 310 is connected to the end of the second telescopic tube away from the bottom plate 310. The end of the second connecting rod 160 away from the first sliding cylinder 121 is installed with a pressing rod 140. The end of the pressing rod 140 close to the bottom plate 310 is installed with a third sliding cylinder 141. An air groove is installed in the third sliding cylinder 141. The end of the pressing rod 140 close to the bottom plate 310 is installed in the air groove. The end of the third sliding cylinder 141 close to the bottom plate 310 is installed with a needle 142.The conveying assembly 200 includes four first brackets 220 installed at one end of the bottom plate 310 close to the clamping block 110, and the conveying belt 210 is installed on the four brackets. The conveying belt 210 is located directly below the clamping block 110. The end of the pressing rod 140 away from the bottom plate 310 is installed with a gas rod 171, and the end of the gas rod 171 away from the pressing rod 140 is installed with a cylinder 170. The needle 142 is connected to the air groove, and four connecting holes 143 are opened on the needle 142. A connecting hole 143 is connected to the pinhole of the pillow itself. During operation, the fish is directly placed on the conveyor belt 210, which is driven by an external power mechanism. When the fish on the conveyor belt 210 moves to the lower end of the clamping block 110, the power of the conveyor belt 210 is turned off to stop it from rotating. Then, the air cylinder 170 is controlled to push the air rod 171 downward. When the air rod 171 moves downward, it drives the pressing rod 140 at the bottom to move downward. When the pressing rod 140 moves downward, it drives the third sliding cylinder at the lower end to move downward. 141 and the needle 142 at the bottom move downward, and when the clamping block 110 clamps the fish on the conveyor belt 210, the needle 142 will also synchronously contact the body of the fish and collect part of the body of the fish. When the collection is completed, the control cylinder 170 will retract the air rod 171 upward. During the recovery, all the devices at the lower end will move synchronously, the clamping block 110 will also lose the clamping effect on the fish, and synchronously, the needle 142 will also leave the body of the fish. Then, in the process of moving upward, the first sliding cylinder 1 The first spring 122 in the 21 will push the telescopic rod to drive the clamping block 110 to reset for the next collection work. At the same time, the second spring 132 will push the second telescopic rod 131 downward. When the second telescopic rod 131 is pushed downward, it drives the third sliding cylinder 141 to move downward synchronously through the first connecting rod 150. Since the third sliding cylinder 141 and the pressing rod 140 form a piston mechanism, a part of the body will be adsorbed into the third sliding cylinder 141, and the third sliding cylinder 141 will be reset at the same time. ;

[0034] A rotating disk 350 is installed at one end of the three cylinders 170 away from the bottom plate 310, a transmission shaft 340 is installed at one end of the rotating disk 350 away from the bottom plate 310, a motor 330 is installed at one end of the transmission shaft 340 away from the bottom plate 310, a right-angle support frame 320 is installed at one end of the motor 330 away from the bottom plate 310, the lowermost end of the right-angle support frame 320 is connected to one end of the bottom plate 310 close to the motor 330, a cleaning box 360 is installed at one end of the bottom plate 310 close to the right-angle support frame 320, four second brackets 370 are installed at one end of the bottom plate 310 close to the cleaning box 360, a workbench 380 is installed at one end of the four second brackets 370 away from the bottom plate 310, and a collecting cylinder 390 is installed at one end of the workbench 380 away from the second brackets 370. When working, the motor 330 is started and the electric The machine 330 drives the transmission shaft 340 to rotate, and the transmission shaft 340 drives the rotating disk to rotate while rotating. The rotating disk then drives the three cylinders 170 and all the devices at the lower end of the cylinder 170 to rotate. When the needle 142 at the upper end of the conveyor belt 210 collects downward, the needle 142 at the upper end of the collecting cylinder 390 will also descend synchronously and enter the collecting cylinder 390. Then, when the pressing rod 140 enters the third sliding cylinder 141, the fish body in the third sliding cylinder 141 is squeezed out into the collecting cylinder 390. Then, the cylinder 170 retracts the air rod 171 upward. At the same time, the cylinder 170 at the upper end of the cleaning box 360 synchronously presses all the devices at the lower end downward, and when it moves downward, the needle 142 part will touch the disinfectant in the main cleaning box 360 to achieve the disinfection effect on the needle 142.

[0035] Working principle: During operation, the fish are directly placed on the conveyor belt 210, which is driven by an external power mechanism. When the fish on the conveyor belt 210 moves to the lower end of the clamping block 110, the power supply of the conveyor belt 210 is turned off to stop the rotation. Then, the air cylinder 170 is controlled to push the air rod 171 downward. When the air rod 171 moves downward, it drives the pressing rod 140 at the bottom to move downward. When the pressing rod 140 moves downward, it drives the third sliding cylinder 141 at the lower end and the needle 142 at the bottom to move downward. When the clamping block 110 clamps the fish on the conveyor belt 210, The needle 142 will also synchronously contact the body of the fish and collect part of the body of the fish. When the collection is completed, the control cylinder 170 will retract the air rod 171 upward. During the recovery, all the devices at the lower end will move synchronously, and the clamping block 110 will also lose its clamping effect on the fish. Synchronously, the needle 142 will also leave the body of the fish. Then, in the process of moving upward, the first spring 122 in the first sliding cylinder 121 will push the telescopic rod to drive the clamping block 110 to reset for the next step of collection. At the same time, the second spring 132 will push the second telescopic rod 131 When the second telescopic rod 131 is pushed downward, the third sliding cylinder 141 is driven to move downward synchronously through the first connecting rod 150. Since the third sliding cylinder 141 and the pressing rod 140 form a piston mechanism, a part of the body is adsorbed into the third sliding cylinder 141, and the third sliding cylinder 141 is reset at the same time. Subsequently, the motor 330 is started, and the motor 330 drives the transmission shaft 340 to rotate. When the transmission shaft 340 rotates, it drives the rotating disk to rotate. The rotating disk then drives the three cylinders 170 and all the devices at the lower end of the cylinder 170 to rotate, and the conveyor belt 210 is moved upward. When the needle 142 at the end moves downward to collect, the needle 142 at the upper end of the collecting cylinder 390 will also move downward synchronously and enter into the collecting cylinder 390, and then the pressing rod 140 will squeeze the fish body in the third sliding cylinder 141 outward into the collecting cylinder 390 when entering into the third sliding cylinder 141, and then the cylinder 170 will retract the air rod 171 upward, and at the same time, the cylinder 170 at the upper end of the cleaning box 360 will synchronously press all the devices at the lower end downward, and when it moves downward, the needle 142 will touch the disinfectant in the main cleaning box 360 to disinfect the needle 142.

[0036] It is understood that the present invention is described by some embodiments, and those skilled in the art are aware that various changes or equivalent substitutions may be made to these features and embodiments without departing from the spirit and scope of the present invention. In addition, under the teachings of the present invention, these features and embodiments may be modified to adapt to specific circumstances and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application belong to the scope protected by the present invention.

Claims

1. A fully automatic collection device for freshwater fish eDNA samples, characterized in that: include: A support assembly (300), the support assembly (300) comprising a bottom plate (310); A collection component (100), the collection component (100) comprising three clamping blocks (110) mounted above a bottom plate (310), a first telescopic rod (120) being mounted on one end of any of the clamping blocks (110) away from the bottom plate (310), a first sliding cylinder (121) being mounted on the first telescopic rod (120), a first spring (122) being mounted inside the first sliding cylinder (121), an end of the first spring (122) close to the bottom plate (310) being connected to an end of the first telescopic rod (120) away from the bottom plate (310), a second connecting rod (160) being mounted on one end of the first sliding cylinder (121), and a second sliding cylinder (130) being mounted on one end of the second connecting rod (160) close to the bottom plate (310), A second telescopic rod (131) is installed in the second sliding cylinder (130), a second spring (132) is installed in the second sliding cylinder (130), one end of the second spring (132) close to the bottom plate (310) is connected to one end of the second telescopic tube away from the bottom plate (310), a pressing rod (140) is installed at one end of the second connecting rod (160) away from the first sliding cylinder (121), a third sliding cylinder (141) is installed at one end of the pressing rod (140) close to the bottom plate (310), an air groove is installed in the third sliding cylinder (141), one end of the pressing rod (140) close to the bottom plate (310) is installed in the air groove, and one end of the third sliding cylinder (141) close to the bottom plate (310) is installed with a needle (142); A conveying assembly (200) includes four first brackets (220) installed on a bottom plate (310) close to one end of a clamping block (110), and conveying belts (210) are installed on the four brackets. The conveying belts (210) are located directly below the clamping block (110).

2. The fully automatic collection device for freshwater fish eDNA samples according to claim 1, characterized in that: An air rod (171) is installed at one end of the pressing rod (140) away from the bottom plate (310), and an air cylinder (170) is installed at one end of the air rod (171) away from the pressing rod (140).

3. The fully automatic collection device for freshwater fish eDNA samples according to claim 1, characterized in that: The needle head (142) is connected to the air groove, and four connecting holes (143) are provided on the needle head (142), and any one of the connecting holes (143) is connected to the needle hole of the pillow itself.

4. The fully automatic collection device for freshwater fish eDNA samples according to claim 2 is characterized in that: A rotating disk (350) is installed at one end of the three cylinders (170) away from the bottom plate (310), a transmission shaft (340) is installed at one end of the rotating disk (350) away from the bottom plate (310), a motor (330) is installed at one end of the transmission shaft (340) away from the bottom plate (310), a right-angle support frame (320) is installed at one end of the motor (330) away from the bottom plate (310), and the lowermost end of the right-angle support frame (320) is connected to one end of the bottom plate (310) close to the motor (330).

5. The fully automatic collection device for freshwater fish eDNA samples according to claim 1, characterized in that: A cleaning box (360) is installed at one end of the bottom plate (310) close to the right-angle support frame (320).

6. The fully automatic collection device for freshwater fish eDNA samples according to claim 1, characterized in that: Four second brackets (370) are installed at one end of the bottom plate (310) close to the cleaning box (360), a workbench (380) is installed at one end of the four second brackets (370) away from the bottom plate (310), and a collecting cylinder (390) is installed at one end of the workbench (380) away from the second brackets (370).

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