Integrated sieve piece basket and reactor for adherent cell culture

Abstract

The utility model relates to the technical field of cell culture, and aims to solve the technical problem that a sieve piece basket is a disposable consumable and is high in cost. The cell attachment surface area is limited, and the cell culture effect is poor. In order to solve the technical problem, the utility model provides the integrated sieve piece basket for adherent cell culture and the reactor. The vibrating screen comprises an upper supporting screen piece and a lower supporting screen piece, the lower supporting screen piece is provided with a plurality of lower through holes; the supporting cylinder is vertically arranged and is used for connecting the upper supporting screen piece and the lower supporting screen piece; the brush assembly comprises a plurality of brushes, the brushes are arranged in a plurality of circles, and a plurality of brushes are arranged in one circle; the brush is arranged between the upper supporting screen piece and the lower supporting screen piece. The brush comprises a vertically arranged main body and a plurality of bristles, and the bristles are spirally wound on the main body. According to the utility model, the cell attachment surface area is increased, and the mixing efficiency and the cell density are improved; the device can be repeatedly used, and the material cost required by each experiment is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to an integrated sieve basket and a reactor for culture of adherent cells. Background Art

[0002] The design and application of cell carriers and bioreactors aims to improve the efficiency and yield of cell culture through innovative technical means to meet the high standards of biotechnology product production. Through the analysis and improvement of existing technologies, the development of the bioreactor field can be further promoted to provide more efficient and reliable solutions.

[0003] The cultivation of adherent-dependent cells is a complex process, and multiple factors need to be considered to ensure the reliability and repeatability of experimental results. Dependent cells need to be attached to a carrier to grow. Currently, most of the microcarriers on the market are disposable, which are difficult to digest and amplify cells. Cost control is particularly critical in large-scale production. By optimizing the design and production process of microcarriers, production costs can be reduced and production capacity per unit volume can be increased. However, the sieve baskets in existing adherent cell culture reactors are disposable consumables with high costs; the cell attachment surface area is limited, and the cell culture effect is poor. Utility Model Content

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the above-mentioned problems existing in the prior art.

[0005] In order to solve the above technical problems, the utility model provides an integrated sieve basket for adherent cell culture, comprising:

[0006] The upper supporting screen is provided with a plurality of upper through holes;

[0007] The lower supporting screen is provided with a plurality of lower through holes;

[0008] A support cylinder, arranged vertically and used to connect the upper support screen plate and the lower support screen plate;

[0009] The brush assembly comprises a plurality of brushes, which are arranged in a plurality of circles, and a plurality of brushes are arranged in one circle; the brushes are arranged between an upper supporting screen and a lower supporting screen; the brush comprises a vertically arranged main body and a plurality of bristles, and the plurality of bristles are spirally wound on the main body.

[0010] In one embodiment of the utility model, the upper through holes are divided into multiple groups, each group is provided with multiple upper through holes; the multiple upper through holes in one group of upper through holes are arranged at intervals along the circumference of the main body and around the axis thereof.

[0011] In one embodiment of the present invention, the upper through holes and the lower through holes are arranged in a one-to-one correspondence.

[0012] In one embodiment of the present invention, one end of the bristle is connected to the main body, and the other end extends outwardly along the radial direction of the main body.

[0013] In one embodiment of the utility model, the present application further comprises a plurality of guide tubes arranged in parallel with the support tube, and the guide tubes connect the upper support screen plate and the lower support screen plate.

[0014] In one embodiment of the present invention, the top of the guide tube extends to above the upper supporting screen.

[0015] In one embodiment of the present invention, the upper supporting screen is made of 316L stainless steel.

[0016] In one embodiment of the present invention, the lower supporting screen is made of 316L stainless steel.

[0017] In one embodiment of the present invention, the support tube is made of 316L stainless steel.

[0018] In one embodiment of the present invention, the guide tube is made of 316L stainless steel.

[0019] In one embodiment of the present invention, the main body is made of 316L stainless steel.

[0020] The utility model provides a reactor, comprising:

[0021] A tank body having an opening at the top;

[0022] The integrated sieve basket for adherent cell culture in any of the above embodiments is installed in the tank body; both ends of the support tube of the integrated sieve basket for adherent cell culture extend outside the upper support sieve and the lower support sieve respectively;

[0023] The stirring mechanism is connected to the outer wall of the top end of the supporting cylinder.

[0024] In one embodiment of the utility model, the upper supporting sieve and the lower supporting sieve of the integrated sieve basket for adherent cell culture are both provided with sealing rings;

[0025] A plurality of legs are provided at the bottom of the lower supporting screen plate;

[0026] Among them, the integrated sieve basket used for adherent cell culture is supported in the tank body by legs and connected to the tank body by a sealing ring.

[0027] The above technical solution of the utility model has the following advantages compared with the prior art:

[0028] The utility model discloses an integrated sieve basket and a reactor for cell culture on the wall. The brush is arranged in this embodiment so that the cells are adsorbed on the bristles. Since the present application provides multiple brushes, and each brush is provided with multiple spirally arranged bristles, the surface area of ​​cell attachment is greatly increased, so that the uniformity of the mixing of cells and culture medium is improved, thereby improving the mixing efficiency and the density of cells; it can effectively improve the cell density and yield per unit volume, thereby improving production efficiency. In addition, the upper support sieve and the lower support sieve of this embodiment are respectively provided with upper through holes and lower through holes, so that the culture medium flows up and down in the upper through holes and the lower through holes during the stirring process, so that the liquid (culture medium) in the tank is mixed up and down, thereby having good oxygen mass transfer and mixing uniformity, meeting the environmental requirements of cell production. The bristles in this embodiment are made of PET material. On the one hand, after the experiment is completed, the old cells can be removed by appropriate means (such as cleaning, disinfection, decellularization, etc.), and then sterilized at high temperature and high pressure for a new round of cell culture, so that it can be reused, reducing the material cost required for each experiment, which is more economical in the long run and reduces the consumption of disposable consumables. Secondly, the material of the bristles has good biocompatibility, which is convenient for adding digestive agents into the tank and dissolving cells in the liquid at a low speed. In addition, the embodiment can be purchased once and used multiple times, which reduces the workload of frequently changing the carrier and simplifies the experimental process. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to make the content of the utility model easier to understand, the utility model is further described in detail according to the specific embodiments of the utility model in combination with the accompanying drawings, wherein:

[0030] Figure 1 This is a schematic diagram of the structure of a reactor in a preferred embodiment of the utility model;

[0031] Figure 2 yes Figure 1 A schematic diagram of the structure of an integrated screen basket and tank for adherent cell culture in a reactor;

[0032] Figure 3 yes Figure 1 A schematic diagram of the structure of an integrated screen basket for adherent cell culture in a reactor;

[0033] Figure 4 yes Figure 3 The main view;

[0034] Figure 5 yes Figure 3 Schematic diagram of the structure of the medium brush;

[0035] Figure 6 yes Figure 5 The main view;

[0036] Figure 7 yes Figure 5 A top view of

[0037] Description of the accompanying drawings in the specification: 100, tank body;

[0038] 200, screen basket; 210, upper support screen; 211, upper through hole; 220, lower support screen; 221, lower through hole; 230, support cylinder; 240, brush assembly; 241, brush; 2411, main body; 2412, brush bristles; 250, guide tube;

[0039] 300, stirring mechanism;

[0040] 400. Outrigger. DETAILED DESCRIPTION

[0041] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0042] Reference Figure 1 to Figure 7 As shown, the embodiment of the utility model provides an integrated screen basket and a reactor for adherent cell culture, comprising:

[0043] A tank body 100 , an integrated sieve basket 200 for adherent cell culture, and a stirring mechanism 300 .

[0044] The top of the tank body 100 is provided with an opening.

[0045] The integrated sieve basket 200 for adherent cell culture includes: an upper support sieve 210, a lower support sieve 220, a support cylinder 230 and a brush assembly 240. The upper support sieve 210 is provided with a plurality of upper through holes 211; the lower support sieve 220 is provided with a plurality of lower through holes 221; the support cylinder 230 is vertically arranged and used to connect the upper support sieve 210 and the lower support sieve 220; the brush assembly 240 includes a plurality of brushes 241, the brushes 241 are arranged in multiple circles, and a plurality of brushes 241 are arranged in one circle; the brushes 241 are arranged between the upper support sieve 210 and the lower support sieve 220; the brush 241 includes a vertically arranged main body 2411 and a plurality of bristles 2412, and the plurality of bristles 2412 are spirally wound on the main body 2411; the bristles 2412 are made of PET material. The sieve basket 200 allows cells to attach, grow and proliferate.

[0046] The integrated sieve basket 200 for adherent cell culture is installed in the tank body 100; the two ends of the support tube 230 of the integrated sieve basket 200 for adherent cell culture extend to the outside of the upper support sieve 210 and the lower support sieve 220 respectively; the stirring mechanism 300 is connected to the outer wall of the top of the support tube 230. The upper support sieve 210 and the lower support sieve 220 are both provided with sealing rings; the bottom of the lower support sieve 220 is provided with a plurality of legs 400; the integrated sieve basket 200 for adherent cell culture is supported in the tank body 100 by the legs 400, and is connected to the tank body 100 by the sealing ring.

[0047] After the cell culture is completed, through pretreatment, cell separation, collection, etc., an appropriate digestive agent is added to decompose the connecting proteins between cells, so that the cells are separated from the brush 241. After the cells are digested for a certain period of time, the stirring mechanism 300 rotates at a low speed to make the cells fully fall off and be collected in the solution. The collected cell suspension needs to be centrifuged to separate the cells, and the cells are washed with fresh culture medium to remove the residual digestive solution to avoid affecting the subsequent cell culture.

[0048] The used brush 241, sieve, stirring mechanism 300 and other equipment need to undergo a strict cleaning procedure to ensure that there are no obvious cell residues and impurities, and then be sterilized at high temperature and high pressure to achieve a sterile state so that they can be used for cell culture again.

[0049] Specifically, the brush 241 in this embodiment is provided so that the cells are adsorbed on the bristles 2412. Since the present application provides a plurality of brushes 241, and each brush 241 is provided with a plurality of spirally arranged bristles 2412, the surface area for cell attachment is greatly increased, so that the uniformity of the mixing of cells and culture medium is improved, thereby improving the mixing efficiency and the density of cells; it can effectively improve the cell density and yield per unit volume, thereby improving production efficiency. In addition, the upper support screen 210 and the lower support screen 220 of this embodiment are respectively provided with an upper through hole 211 and a lower through hole 221, so that during the stirring process, the culture medium flows up and down in the upper through hole 211 and the lower through hole 221, so that the liquid (culture medium) in the tank is mixed up and down, thereby having good oxygen mass transfer and mixing uniformity, meeting the environmental requirements of cell production. The bristles 2412 in this embodiment are made of PET material. On the one hand, after the experiment, the old cells can be removed by appropriate means (such as cleaning, disinfection, decellularization, etc.), and then sterilized again at high temperature and high pressure for a new round of cell culture, so that it can be reused, reducing the material cost required for each experiment. In the long run, it is more economical and reduces the consumption of disposable consumables. Secondly, the material of the bristles 2412 has good biocompatibility, which is convenient for adding digestive agents to the tank 100 and dissolving the cells in the liquid at a low speed. In addition, this embodiment can be purchased once and used multiple times, which reduces the workload of frequently changing the carrier and simplifies the experimental process.

[0050] The bristles 2412 of this embodiment are made of PET material, which has good tolerance and can maintain stable physical and chemical properties after multiple cleaning and sterilization, ensuring the consistency of cell adsorption and growth. It can be seen that the stability and consistency of this embodiment are good. This embodiment can remove residual cells and proteins during cleaning and disinfection, reducing the risk of cell cross contamination.

[0051] In some embodiments, the direction of the spiral arrangement of the bristles 2412 is the same as the stirring direction of the stirring mechanism 300, for example, both are clockwise, so that the flow direction of the material (cell) can be better controlled and the cell adsorption force can be better.

[0052] Further, the upper through holes 211 are divided into multiple groups, each group is provided with multiple upper through holes 211; the multiple upper through holes 211 in one group of upper through holes 211 are arranged at intervals around the axis of the main body 2411 along the circumference thereof. The upper through holes 211 and the lower through holes 221 are arranged one by one in the upper and lower directions. Specifically, the flow of the culture medium is smoother in this way.

[0053] Further, one end of the bristle 2412 is connected to the main body 2411, and the other end extends outwardly along the radial direction of the main body 2411. Specifically, the brush body in this embodiment diverges outward, which is convenient for increasing the contact area of ​​cells in all directions.

[0054] Furthermore, the present application further includes a plurality of guide tubes 250 arranged in parallel with the support cylinder 230 , and the guide tubes 250 connect the upper support screen 210 and the lower support screen 220 . Specifically, the guide tubes 250 provide support for the screen basket 200 .

[0055] Furthermore, the top of the guide tube 250 extends above the upper support screen 210. Specifically, the top of the guide tube 250 of this embodiment extends beyond the upper support screen 210, so that the screen basket 200 can be installed in the tank body 100 or removed from the tank body 100 by holding the end of the guide tube 250, which is convenient and labor-saving.

[0056] Furthermore, the number of the guide tubes 250 is an even number, and pairs of the multiple guide tubes 250 are arranged symmetrically relative to the support tube 230 .

[0057] Further, the upper support screen 210 is made of 316L stainless steel. The support screen is made of 316L stainless steel. The support cylinder 230 is made of 316L stainless steel. The guide tube 250 is made of 316L stainless steel. The main body 2411 is made of 316L stainless steel. The material of the main body 2411 has good corrosion resistance and mechanical properties and is easy to clean.

[0058] Obviously, the above embodiments are merely examples for the purpose of clear explanation and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention of the utility model.

Claims

1. An integrated screen basket for adherent cell culture, characterized in that: include: The upper supporting screen is provided with a plurality of upper through holes; The lower supporting screen is provided with a plurality of lower through holes; A support cylinder, arranged vertically and used to connect the upper support screen plate and the lower support screen plate; The brush assembly includes multiple brushes, which are arranged in multiple circles, with multiple brushes arranged in one circle; the brushes are arranged between the upper supporting screen and the lower supporting screen; the brush includes a vertically arranged main body and multiple bristles, and the multiple bristles are spirally wound on the main body; the bristles are made of PET material.

2. The integrated sieve basket for adherent cell culture according to claim 1, characterized in that: The upper through holes are divided into a plurality of groups, each group being provided with a plurality of upper through holes; the plurality of upper through holes in a group of upper through holes are arranged at intervals along the circumference of the main body and around the axis thereof.

3. The integrated screen basket for adherent cell culture according to claim 2, characterized in that: The upper through holes and the lower through holes are arranged one by one in upper and lower directions.

4. The integrated screen basket for adherent cell culture according to claim 1, characterized in that: One end of the bristle is connected to the main body, and the other end extends outwardly along the radial direction of the main body.

5. The integrated screen basket for adherent cell culture according to claim 1, characterized in that: It also includes a plurality of guide tubes arranged in parallel with the support tube, and the guide tubes are connected to the upper support screen plate and the lower support screen plate.

6. The integrated screen basket for adherent cell culture according to claim 5, characterized in that: The top of the guide tube extends to above the upper supporting screen.

7. The integrated screen basket for adherent cell culture according to claim 5, characterized in that: The upper supporting screen is made of 316L stainless steel; And / or the lower supporting screen is made of 316L stainless steel; And / or the support cylinder is made of 316L stainless steel; And / or the guide tube is made of 316L stainless steel.

8. The integrated screen basket for adherent cell culture according to claim 1, characterized in that: The main body is made of 316L stainless steel.

9. A reactor, characterized in that: include: A tank body having an opening at the top; The integrated sieve basket for adherent cell culture according to any one of claims 1 to 8, which is detachably connected to the tank body; The two ends of the support tube of the integrated sieve basket for adherent cell culture extend outside the upper support sieve and the lower support sieve respectively; The stirring mechanism is connected to the outer wall of the top end of the supporting tube.

10. The reactor according to claim 9, characterized in that: The upper supporting sieve and the lower supporting sieve of the integrated sieve basket for adherent cell culture are both provided with sealing rings; The bottom of the lower supporting screen is provided with a plurality of supporting legs; Wherein, the integrated sieve basket for adherent cell culture is supported in the tank body by legs and connected to the tank body by the sealing ring.

Images